Sphereclone (Phenomenex)

SPHERECLONE
Use SphereClone Columns
as an Alternative to Spherisorb
• Performance guaranteed
SphereClone is packed only by Phenomenex, thereby reducing the
chance of column-to-column variation. You will receive columns
of consistent quality and reproducibility, and will benefit from
Phenomenex’s excellent customer support and technical service.
If SphereClone does not provide at least
an equivalent separation as compared to
Spherisorb columns of the same phase,
particle size and comparable dimensions, send
in your comparative data within 45 days and
keep the SphereClone column for FREE.
Below are listed side-by-side comparisons of physical material
characteristics. As you can see, SphereClone and Spherisorb ®
compare very well for important specifications such as particle
size, pore size, surface area and carbon load.
SphereClone vs. Spherisorb ®†
SEM of Base Silica
Material Characteristics
SEM of Base Silica
SphereClone vs. Spherisorb ®†
3, 5, 10µ Particle Size 3, 5, 10µ
80Å Pore Size 80Å
HPLC
200m 2 /g Surface Area 200m 2 /g
Carbon Load
SphereClone
Surface
15,000x Magnification
— Silica —
6% C6 6%
6% C8 6%
7% ODS(1) 7%
12% ODS(2) 12%
Surface
15,000x Magnification
2% NH 2
2%
3.0% Phenyl 3.0%
Physical Mass
Distribution & Shape
4,000x Magnification
— SCX —
Physical Mass
Distribution & Shape
4,000x Magnification
Particle Size Distribution
vs.
SphereClone 5µ Silica
Spherisorb ® 5µ Silica
SphereClone
Reproducibility
Value
QC Data for Silica
Surface Area (m 2 /g)
Pore Volume (mL/g)
Pore Diameter (Å)
Standard Deviation
Particle Diameter
5µ Particle Size Data
Dp number
Dp volume
STD
Batch
Batch
226 Phenomenex

SPHERECLONE
SphereClone
‡
vs.
Spherisorb ®†
5µ ODS(2) 5µ ODS(2)
App ID 5548
mAU
800
700
600
500
400
300
200
100
0
1
2
5µ ODS(2)
6
4
7
5
3
App ID 5549
mAU
800
700
600
500
400
300
200
100
0
1
2
6
4+5
5µ ODS(2)
7
3
Fat-Soluble Vitamins
Dimensions: 150 x 4.6mm
Mobile Phase: Acetonitrile/Methanol (75:25)
Flow Rate: 1.3mL/min
Detection: UV @ 280nm
Sample: 1. Vitamin A
2. Vitamin A Acetate
3. Vitamin D 2
4. Vitamin D 3
5. Vitamin E
6. Vitamin E Acetate
7. Vitamin K 1
App ID 5550
mAU
1000
800
600
400
200
0 5 10 min
3
1
2
5µ ODS(2)
4
5
App ID 5551
mAU
1000
800
600
400
200
0 5 10
1
2
3
4
5
min
5µ ODS(2)
Pesticides/Herbicides
Dimensions: 150 x 4.6mm
Mobile Phase: Water/Acetonitrile (70:30)
Flow Rate: 1.5mL/min
Detection: UV @ 220nm
Sample: 1. Baygon
2. Carbofuran
3. Carbaryl
4. Propham
5. Captan
HPLC
SphereClone
0
0
App ID 5558
mAU
160
140
120
100
80
60
40
20
0
0 10 20 30 min
1
2
‡
vs.
0 10 20 30 min
5µ ODS(1) 5µ ODS(1) +
3
5 m ODS(1)
4
App ID 5559
mAU
140
120
100
80
60
40
20
0
2
3
1 5 m ODS(1)
4
+
Polar Compounds
Dimensions: 250 x 4.6mm
Mobile Phase: 25mM KH 2
PO 4
, pH 2.5
Flow Rate: 1.0mL/min
Temperature: 40°C
Detection: UV @ 230nm
Sample: 1. L-Cysteine
2. L-Ascorbic Acid
3. Glutathione
4. Uric Acid
0 2 4 6 8
min
0 2 4 6 8
min
App ID 5562
mAU
300
250
200
150
100
50
0
1
2
3
4
5 m ODS(1)
App ID 5563
mAU
300
250
200
150
100
50
0
1
2
3
5 m ODS(1)
4
+
Phthalate
Dimensions: 250 x 4.6mm
Mobile Phase: Acetonitrile/Water (70:30)
Flow Rate: 1.0mL/min
Detection: UV @ 230nm
Sample: 1. Dimethyl Phthalate
2. Anisole
3. Naphthalene
4. Dibutyl Phthalate
0 2 4 6 8 10 min
0 2 4 6 8 10 min
Phenomenex
227

SPHERECLONE
App ID 5572
SphereClone Spherisorb ®†
5 m C8
mAU
3
350
2
4
300
250
1
200
150
100
50
‡
vs.
5µ C8 5µ C8 +
App ID 5573
3
mAU
350
2
4
300
250
5 m C8 +
200
1
150
100
50
Polar Compounds
Dimensions: 250 x 4.6mm
Mobile Phase: 25mM KH 2
PO 4
, pH 2.5
Flow Rate: 1.0mL/min
Detection: UV @ 230nm
Sample: 1. L-Cysteine
2. L-Ascorbic Acid
3. Glutathione
4. Uric Acid
0
0
0 2 4 6 8
min
0 2 4 6 8
min
HPLC
SphereClone
App ID 5576
mAU
600
500
400
300
200
100
0
1
2
3
4
5
5µ C8
6
App ID 5577
mAU
600
500
400
300
200
100
0
1
2
4
3
5
5µ C8
6
Water-Soluble Vitamins
Dimensions: 150 x 4.6mm
Mobile Phase: A: 5mM Sodium 1-Pentanesulfonate
in 0.1% H 3
PO 4
B: 5mM Sodium 1- Pentanesulfonate in
0.1% H 3
PO 4
in 80% Acetonitrile
Gradient: A/B (97.5:2.5) to A/B (70:30) in 20 min
Flow Rate: 1.0mL/min
Temperature: 40°C
Detection: UV @ 254nm
Sample: 1. Niacinamide
2. Pyridoxal
3. p-Aminobenzoic Acid
4. Thiamine
5. Folic Acid
6. Riboflavin
0 5 10
min
0 5 10
min
App ID 5590
mAU
200
175
150
125
100
75
50
25
1
2
vs.
‡
mAU
5µ C6 5µ C6
200
2
1
150
5µ C6
5µ C6
100
App ID 5591
50
Endorphins
Dimensions:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Sample:
250 x 4.6mm
A: 0.1% TFA in Water
B: 0.1% TFA in Acetonitrile/Water
(90:10)
A:B-70:30 to A:B-55:45 in 15 min
1.0mL/min
UV @ 214nm
1. Met Enkephalin
2. Leu Enkephalin
0
0
0 2 4 6 8 10 12
min
0 2 4 6 8 10 12 min
App ID 5592
mAU
175
150
125
100
75
50
25
0
1
2
3
4
6
7
5µ SAX
9
8
0 5 10 15 20 25 min
5
‡
vs.
5µ SAX 5µ SAX +
App ID 5593
mAU
200
175
150
125
100
75
50
25
0
1
2
3
4
5
6
9
8
5µ SAX +
0 5 10 15 20 25 min
7
Nucleotides
Dimensions: 250 x 4.6mm
Mobile Phase: A: 0.04M KH 2
PO 4
, pH 5.5
B: 0.5M KH 2
PO 4
, pH 5.5
Gradient: A/B: 100: 0 to A/B 0: 100 in 26 min
Flow Rate: 1.0mL/min
Detection: UV @ 254nm
Sample: 1. β-NAD
2. IMP
3. GMP
4. AMP
5. GDP
6. ADP
7. NADP
8. ITP
9. ATP
228 Phenomenex

SPHERECLONE
App ID 5596
mAU
1
350
2
300
5
3 4
250
200
6
150
100
50
‡
vs.
SphereClone Spherisorb ®†
5µ NH 2
5µ NH 2
App ID 5597
1
mAU
2
300
3 5
4
250
200
6
150
100
50
Corticosteroids
Dimensions: 250 x 4.6mm
Mobile Phase: Dichloromethane/Methanol (95:5)
Flow Rate: 1.0mL/min
Detection: UV @ 254nm
Sample: 1. Deoxycorticosterone Acetate
2. Deoxycorticosterone
3. Hydrocortisone 21-Acetate
4. Corticosterone
5. Cortisone
6. Hydrocortisone
0
0
App ID 5602
mAU
175
150
125
100
75
50
25
0
0 2 4 6 8
1 2
5
3 4
6
7
8
min
‡
vs.
App ID 5603
mAU
140
120
100
80
60
40
20
0
0 2 4 6 8
5µ Phenyl 5µ Phenyl +
1
2
3
4
5
7
6
min
8
Aromatic Ketones
Dimensions: 250 x 4.6mm
Mobile Phase: Acetonitrile/Water (33:67)
Flow Rate: 1.0mL/min
Detection: UV @ 254nm
Sample: 1. Benzamide
2. Benzyl Alcohol
3. Acetophenone
4. Methyl Benzoate
5. Phenetole
6. Naphthalene
7. Benzophenone
8. Biphenyl
HPLC
SphereClone
1 5 10 15 20 min
0 5 10 15 20 25 min
ORDERING INFORMATION
SecurityGuard Cartridges require universal holder Order No.: KJO-4282
3µ Columns (mm) SecurityGuard Cartridges
150 x 1.0 100 x 2.0 150 x 2.0 100 x 3.2 150 x 3.2 100 x 4.6 150 x 4.6 4 x 2.0mm 4 x 3.0mm
Phases
/10pk /10pk
Silica I — I — I — I — I — I 00D-4130-E0 I 00F-4130-E0 AJO-4347 I AJO-4348
C6 — — 00F-4132-B0 — — 00D-4132-E0 00F-4132-E0 — —
C8 — — — 00D-4133-R0 — 00D-4133-E0 — AJO-4289 AJO-4290
ODS (1) — 00D-4134-B0 00F-4134-B0 00D-4134-R0 00F-4134-R0 00D-4134-E0 00F-4134-E0 AJO-4286 AJO-4287
ODS(2) 00F-4135-A0 00D-4135-B0 00F-4135-B0 00D-4135-R0 00F-4135-R0 00D-4135-E0 00F-4135-E0 AJO-4286 AJO-4287
NH 2
— — — — 00F-4137-R0 — 00F-4137-E0 AJO-4301 AJO-4302
Phenyl — — — — — 00D-4138-E0 00F-4138-E0 AJO-4350 AJO-4351
for ID: 2.0-3.0mm 3.2-8.0mm
5µ Columns (mm) SecurityGuard Cartridges
250 x 2.0 150 x 3.2 250 x 3.2 150 x 4.6 250 x 4.6 250 x 10.0 250 x 21.2 4 x 2.0mm 4 x 3.0mm 10 x 10mm
Phases
Silica I 00G-4139-B0 I /10pk /10pk /3pk
— I — I 00F-4139-E0 I 00G-4139-E0 I — I — AJO-4347 I AJO-4348 I AJO-7223
C6 — — — 00F-4141-E0 00G-4141-E0 00G-4141-N0 — — — —
C8 00G-4142-B0 00F-4142-R0 00G-4142-R0 00F-4142-E0 00G-4142-E0 00G-4142-N0 — AJO-4289 AJO-4290 AJO-7222
ODS (1) 00G-4143-B0 00F-4143-R0 00G-4143-R0 00F-4143-E0 00G-4143-E0 00G-4143-N0 — AJO-4286 AJO-4287 AJO-7221
ODS (2) 00G-4144-B0 00F-4144-R0 00G-4144-R0 00F-4144-E0 00G-4144-E0 00G-4144-N0 00G-4144-P0 AJO-4286 AJO-4287 AJO-7221
NH 2
— — 00G-4147-R0 00F-4147-E0 00G-4147-E0 00G-4147-N0 — AJO-4301 AJO-4302 AJO-7364
Phenyl — — — 00F-4148-E0 00G-4148-E0 — — AJO-4350 AJO-4351 AJO-7314
SAX 00G-4149-B0 00F-4149-R0 00G-4149-R0 00F-4149-E0 00G-4149-E0 00G-4149-N0 — AJO-4310 AJO-4311 AJO-7370
for ID: 2.0-3.0mm 3.2-8.0mm 9-16mm
10µ Columns (mm) SecurityGuard Cartridges
250 x 4.6 4 x 3.0mm
‡
Comparative separations may not be representative of all applications.
SphereClone is a trademark of Phenomenex.
Phases
/10pk
Phenomenex is a registered trademark.
+
C8 I 00G-4154-E0 AJO-4290
Spherisorb columns used for comparison studies were
ODS (1)
ODS (2)
00G-4155-E0
00G-4156-E0
AJO-4287
AJO-4287
purchased from manufacturer.
†
Spherisorb is a registered trademark of Waters.
Phenomenex has no affiliation with Waters.
SAX 00G-4160-E0 AJO-4311
for ID: 3.2-8.0mm
See p. 213 for Semi-prep SecurityGuard Cartridge Holders
C1, CN, SCX not currently available. See PhenoSphere on p. 193.
Other dimensions from microbore/SFC to preparative columns
available upon request.
Phenomenex
229