Muller Cell Expression of Gliol Fibrillory Acidic Protein offer Genetic ...

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1324 INVESTIGATIVE OPHTHALMOLOGY & VI5UAL SCIENCE / November 1984 Vol. 25 Fig. 2, Fluorescence micrographs of control and constant light-damaged retinas treated with antibodies to GFAP. A, Control section, exposed to constant light for 3 days and treated with preimmune serum. B, Normal retina. Only astrocytes (—•) stain for GFAP. C, Retina exposed to constant light for 3 days. Miiller cell processes (—') express GFAP immunoreactivity. D, Retina exposed to constant light for 2 weeks. Miiller cells (—>) stain for GFAP. ON, outer nuclear layer (X576). against the inner limiting membrane, and in the ganglion cell and innermost inner plexiform layer. Although the amount of photoreceptor cell loss varied from animal to animal after 3 days in CL, every retina examined showed Miiller cell staining. The staining pattern remained the same at 2 weeks in CL
No. 11 GFAP IN MULLEP, CELLS / Eisenfeld er d. 1325 tig. 3. Huorescence micrographs of RCS retinas treated with antibodies to GFAP. A, Control section. A 38-day-old retina treated with preimmune serum. B, A 25-day-old RCS retina. Only astrocytes (—») express GFAP immunoreactivity. C, A 38-day-old RCS retina. Muller cell processes (—») first stain for GFAP at this age. D, A 6-month-old RCS retina. At this later stage of degeneration, Muller cells (—•) stain intensely for GFAP. Accumulations of lipofuscin (•) are seen in the pigment epithelium. ON, outer nuclear layer (X576). (Fig. ID) and at 8 wk when very few photoreceptors could be found. In the RCS rat, the GFAP staining was restricted to the astrocytes until day 32, as seen in control retinas (Figs. 2B, 3B). Beginning on day 32, an occasional Muller fiber stained lightly for GFAP. Muller cell processes throughout the retina were stained consistently with anti-GFAP only after day 38 (Fig. 3C). At this time the Muller end feet and innermost Muller radial processes stained most intensely. As the photoreceptor degeneration progressed, the number of GFAP positive fibers increased, and they spanned the retina from the inner to the external limiting membranes. At advanced stages of degeneration (6 months; Fig. 3D), the Muller processes were thickened and very heavily stained. Characterization of anti-GFAP In order to ascertain that the protein stained by anti-GFAP was indeed GFAP, electroblot analysis was performed on proteins from normal and degenerated retinas. Results from the anti-GFAP electroblot experiments are presented in Figure 4. Although
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