(PIC) Protocol Manual - Kitsap Public Health District

Kitsap Public Health District
Pollution Source Identification and Correction: Protocol Manual
Fill bottle to the 100 ml line
Note any unusual odors, matting, vegetative growth, laundry lint, food waste, temperature,
animal tracks, animal waste, or any other characteristics that may indicate a sewage or laundry source at
or near the water sample.
Once the sample is collected and the bottle secured, place the bottle in a cooler with ice packs. The
bottles should be kept below 10 degrees Celsius (Holding time and temperature from APHA Standard
Methods, 20 th edition pg 9-21).
Wash hands as soon as possible after sampling and before you eat.
Labeling and Recording samples:
• Use a black permanent marker (or regular pen) to label the 100 milliliter sample bottle with the
sample identifier, date, and time the sample was collected on the label.
• Clearly record the sample name, collection time, location, drainage size, pipe diameter, and pipe
material (if applicable) in the field notebook.
• Record detailed parcel-oriented sample descriptions in the field notebook so that outfalls can be
re sampled by different staff, if necessary.
Transporting samples
When water samples are collected they should be transported in coolers with ice packs. The
recommended temperature, according to APHA Standards Method is 10 degrees Celsius.
Chain of custody
Water samples are analyzed at Lab/Cor Inc. Following the collection of all water samples, a chain of
custody form must be completed. Check the QAPP, or ask the project lead or the Field Supervisor to
verify whether samples should be analyzed for FC or EC. A chain of custody form is also submitted when
additional analytical sampling is needed, e.g. ammonia, nutrients etc.
Revised 04/23/2013 14