(PIC) Protocol Manual - Kitsap Public Health District

Kitsap Public Health District
Pollution Source Identification and Correction: Protocol Manual
In lakes, you may want to establish charcoal floats in priority areas where you plan to dye test.
Two charcoal packs
__ Wear gloves when handling or introducing dye. Dispose of gloves and used dye bottles in a manner
that will prevent cross contamination of dye testing materials. For example, after introducing dye into
fixtures in a house, place empty dye bottle along with gloves into the Ziploc bag that was used to store
the dye bottle.
__Use two charcoal packs to make up each sampler. The duplicate can be stored frozen and sent to OUL
for spectrofluorophotometric analysis as necessary.
__ Charcoal packs should be placed in the same location as the control and in one or more locations
down gradient of the potential source. Remember that the sample locations in flowing water must be in
areas where the water samples results exceeded 200 FC/100ml or 160 EC/100ml. To protect yourself
when placing charcoal packs in a location that has yielded high bacteria counts gloves may be worn.
__ Prior to introducing dye into the OSS, retrieve the controls and seal them into Whirl-Pak TM bags
clearly labeled in black permanent marker with sampler address, location, and dates and times placed
and retrieved.
__Store retrieved charcoal packs away from dye supplies and out of the sun. A clean cooler and ice
packs are used to transport the retrieved charcoal packs to the office. Store them in a dedicated freezer
until elution is performed. The retrieved packs are stored frozen to prevent algal growth that interferes
with dye identification.
__After retrieving the controls, place another set of charcoal packs in the same location. Note the date
and time the controls were retrieved and the new packs replaced on the PIC survey form.
__Map charcoal sampler locations in a field notebook or on the PIC survey form.
Revised 04/23/2013 16