CAFEINE CAS : 58-08-2 - UNEP Chemicals

OECD SIDS CAFFEINE
5. TOXICITY DATE: 04-MAR-2003
SUBSTANCE ID: 58-08-2
Type: Sister chromatid exchange assay
System of testing: human xeroderma pigmentosum cell lines (XP19, XP20)
Concentration: ca. 19, 97, 194 ug/ml (0.1, 0.5, 1.0 mM)
Metabolic activation: without
Result: positive
Method: other: no data
Year: 1988
GLP: no data
Test substance: other TS
Remark: A marked increase in the frequency of SCE was observed at
concentrations of 0.1 to 1 mM in both cell lines.
Test substance: caffeine
Reliability: (3) invalid
unsuitable test system, tumor cell line
07-DEC-2001 (203)
Type: Cytogenetic assay
System of testing: human diploid fibroblasts (WI-38)
Concentration: ca. 252, 505, 757 ug/ml (1.3, 2.6, 3.9 mM)
Metabolic activation: without
Result: positive
Method: other: no data
Year: 1977
GLP: no
Test substance: other TS
Remark: Mitotic index was reduced to 58.8% at 1.3 mM with
significant clastogencity (breaks and gaps); no exchange
figures were seen (incubation with the test substance for 24
h, colcemid added 24 h later, scoring of 200 metaphases per
concentration).
Test substance: caffeine
Reliability: (3) invalid
documentation unsufficient
06-DEC-2001 (204)
Type: Cytogenetic assay
System of testing: human diploid fibroblasts (WI-38)
Concentration: ca. 1.0, 1.5, 2.0 mg/ml (5.1, 7.7, 10.3 mM)
Metabolic activation: with and without
Result: positive
Method: other: no data
GLP: no
Test substance: other TS
Remark: Cytogenetic assay with and without metabolic activation with
S-9 mix prepared from livers of Aroclor-pretreated rats
(incubation for 24 h, then incubation with the test
substance in the presence and absence of S-9 for 1 h,
colcemid added 24 h later, scoring of 100 metaphases per
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