3B Scientific - Biology Catalog

Experiments Electrophoresis
9982-1005939
Diagnosing Gene Defects
Examine the genetic mutation responsible for sickle cell anaemia. Your
students will use agarose gel electrophoresis to study sickle cell anaemia –
a painful and ultimately fatal condition resulting from a genetic mutation
which alters the body’s haemoglobin. They’ll search for changes in a nonhuman
DNA sample to diagnose sickle cell anaemia. In the process, they’ll
learn about genes and how genetic mutations can cause disease.
Scope of delivery:
4 DNA samples (mother’s DNA, father’s DNA, daughter’s DNA, unborn
child’s DNA), each 150 ml, 2 TBE Buffer concentrate (125 ml) 10X, 1 melt
and bast agarose gel (400 ml) 0.8%, 1 Neo/BLUE DNA stain concentrate
(100 ml) 10X, 1 staining tray, description.
30x22x10 cm, 1.5 kg
& D/E
9982-1005937
Page 114
9982-1005935
Simulating DNA Paternity Testing
Use agarose gel electrophoresis to test family ties. Simulate how DNA fingerprinting
can be used to identify the genetic relationship between child
and an alleged father. Your class will use the results of an electrophoresis
of non-human DNA, their knowledge of human inheritance and their scientific
problem solving skills to solve a scenario-based paternity case and determine
the identify of a child’s father.
Scope of delivery:
3 DNA samples (mother’s DNA, alleged father’s DNA, child’s DNA) each
150 ml, 2 TBE buffer concentrate (125 ml) 10X, 1 melt and cast agarose gel
(400 ml) 0.8%, 1 Neo/BLUE DNA stain concentrate (100 ml) 10X, 1 staining
tray, description.
30x22x10 cm; 1.5 kg
& D/E/S
9982-1005939
9982-1005937
Genetic Diagnosis of Cancer
Your students will learn how revolutionary breakthroughs in gene technology
can be used to not only detect cancer but predict its occurrence based
upon hereditary traits. They’ll use the electrophoresis results of non-human
DNA samples to simulate this procedure. In the process, they’ll detect
a specific cancer and study the hereditary tendencies of the condition.
Scope of delivery:
DNA samples (Mary’s DNA, Samantha’s DNA, Fran’s DNA, normal control
DNA) each 150 ml, 2 TBE Buffer concentrates (125 ml) 10X, 1 melt and cast
agarose gel 0.8% (400 ml, 1 Neo/BLUE DNA stain concentrate (100 ml)
10X,1 staining tray, description.
30x22x10 cm; 1.5 kg
& D/E
9982-1005935
ATTENTION:
This experiment requires an
electrophoresis chamber and
a suitable power supply.
(Page 114)
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