3B Scientific - Biology Catalog

Experiments Thin Layer Chromatography
On the Trail of Flavour Enhancers
An interesting experiment set that deals with a highly topical food issue, and at the
same time is based on an important chemical/biochemical method of separation (thin
layer chromatography – TLC).
Foods containing glutamates can be found in any supermarket,
often hidden behind the labelling (yeast extract,
spices etc.). These include flavour enhancers in foodstuffs,
and their effect on people is a issue that is much
spoken about in the media. In biology class, this topic
can be easily incorporated into lessons on metabolic
physiology, human health and diseases, nutrition and
proteins.
In many syllabuses, carrying out thin layer chromatography is explicitly required.
The pupils not only use this test method in our experiment; they
also think about it and comment on it. Depending on how the lesson is
structured, the pupils can also consider possible errors using this experiment,
by discussing potential sources of error in planning and carrying out
the experiment (e.g. the lack of a control experiment). By making a comparison
with the three glutamic acid standards, the tested samples can be
looked at from both a qualitative and a semi-quantitative perspective. In
the instructions, you will find both a thorough description of the test, and
interesting background information, as the experimentation kit has been
developed in collaboration with a food analysis laboratory.
Scope of delivery:
Extraction solution, L-Glutamic acid 50 mg, ninhydrin 300 mg, 20 pleated
filters, 50 disposable capillaries, 20 chromatography paper, 5 chromatography
chambers, test instructions with interesting background information.
Packaging size: Sufficient for 20 tests, with 5 groups working at the same
time.
& D/E/S/F/I/P
9982-1013460
Brand new and
topical – versatile
in classroom
situations
9982-1013460
140
3B Scientific® Biology