3B Scientific - Biology Catalog
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DNA Fingerprint<br />
DNA fingerprinting is an important method used in molecular genetics. It<br />
is now inconceivable, for instance, for criminal forensics to do without it.<br />
Other examples of areas where it can be applied are paternity tests, the<br />
analysis of genetic diseases, and identifying victims after natural catastrophes<br />
or accidents. During this procedure, DNA fragments are generated by<br />
way of a polymerase chain reaction (PCR), and then separated using gel<br />
electrophoresis. In our kit, however, the DNA fragments have already been<br />
separated so that the pupils only have to carry out electrophoresis. Using<br />
the DNA profiles thus obtained, your pupils can draw their conclusions<br />
about where they come from (scene of the crime, victim, or suspect). While<br />
doing this, the pupils learn about the practicalities of using molecular genetics<br />
techniques, and will be able to discuss the DNA profiles in detail<br />
once they have completed the experiment. This topic is also a wonderful<br />
way of introducing a discussion about other potential applications for DNA<br />
finger printing, as well as about associated legal and ethical issues.<br />
Scope of delivery:<br />
DNA from a victim, 120 µl, DNA from the scene of the crime, 120 µl, DNA<br />
from suspect n° 1, 120 µl, DNA from suspect n° 2, 120 µl, electrophoresis<br />
buffer, 50x conc., 50 ml, agarose, 6 g, DNA staining solution, 200x conc.,<br />
1.5 ml<br />
Packaging size: Enough for 10 experiments<br />
& D/E/F/S/I/P<br />
9982-1013458<br />
Additionally required:<br />
Electrophoresis chamber (9982-1012852, page 114), power supply (9982-<br />
1010263, page 116) and micropipette (9982-1013416, page 111)<br />
Supplies for Electrophoresis Experiments:<br />
Agarose<br />
for DNA gel electrophoresis, compatible with usual electrophoresis buffers<br />
and DNA staining methods, 50 g.<br />
9982-1013341<br />
9982-1013458<br />
ATTENTION:<br />
This experiment requires an<br />
electrophoresis chamber and<br />
a suitable power supply.<br />
(Page 114)<br />
Electrophoresis<br />
Methyl Blue Staining Concentrate (not shown)<br />
Concentrated 200 times (2x 1.5 ml), staining in approx. 15 – 20 min. with a<br />
high level of sensitivity. Sufficient for approx. 20 stainings of medium-sized<br />
gels.<br />
9982-1013342<br />
TAE Electrophoresis Buffer (not shown)<br />
50ml, electrophoresis buffer concentrated 50 times, contains 2M Tris,<br />
1M ascetic acid, 50mM EDTA, pH 8.5.<br />
9982-1013343<br />
9982-1013341<br />
Gel Loading Buffer (not shown)<br />
Concentrated 6 times – 2x 1.5 ml (with glycerine and bromophenol<br />
blue as colour marker).<br />
9982-1013347<br />
100 bp DNA Ladder (not shown)<br />
250μl, ready for use, dissolved in 1x gel loading buffer,<br />
with bromophenol blue as a colour marker. Size range<br />
of the fragments 100 – 1,000 bp. Sufficient for approx.<br />
25 agarose gels.<br />
9982-1013348<br />
Experiments<br />
135