oxidants and antioxidants in biology - Oxygen Club of California

Preconditioned fatty livers are tolerant against normothermic
post-ischemic injury in rats
CAVALIERI, B; PERRELLI, MG.; RAMADORI, P.; GARRONE, A.;
CAVALLO, F.; PAPOTTI, M.*; POLI, G., AND CUTRÍN, J.C.
Laboratory of Experimental Liver Pathology, Department of
Clinical and BiologicalSciences; *Department of Biomedical
Sciences and Oncology, University of Torino
Hepatic fatty change is a well-documented risk factor in reperfusion
injury (1). Ischemic preconditioning (PC) appears as an effective
strategy to attenuate the damage due to ischemia and reperfusion
(2, 3). The aim of the present study was to evaluate wether
PC could be effective in an in vivo rat model of liver steatosis induced
by a choline deficient diet. After a warm cycle of 60 min of
lobar selective ischemia followed by 30 min of reperfusion, a consistent
appearance of necrosis was reproduced in both normal and
fatty livers. Steatosis increased the post-ischemic H 2 O 2 concentration,
as well as the formation of HNE-protein adducts. PC was
found to control both in normal and fatty livers one of the mechanisms
involved in the reperfusion injury, i.e. oxidative stress and
lipid peroxidation. The marked quenching of oxidant generation
and oxidative reactions seem to be one of the pathways by which
PC reduces the reperfusion injury and cell death.
(1) Caraceni P; Nardo B; Domenicali M; Turi P; Vici M; Simoncini
M, et al. Hepatology 1999; 29: 1139-46.
(2) Peralta C; Bulbena O; Xaus C; Prats N; Cutrìn JC; Poli G, et al.
Transplantation 2002; 73: 1203-11.
(3) Cavalieri B; Perrelli MG; Aragno M; Mastrocola R; Corvetti G;
Durazzo M; Poli G and Cutrìn JC. Liver Transplantation 2002; 8:990-9.
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