oxidants and antioxidants in biology - Oxygen Club of California

Histological Evidence for enhanced wound healings in diabetic
rats supplemented with high dose palm vitamin E
MUSALMAH M, NIZRANA MY, MUHD FAIRUZ AH, *GAPOR MT
AND WAN NGAH WZ.
Dept of Biochemistry, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan
Raja Muda Abdul Aziz, 50300 Kuala Lumpur and *Malaysia Palm Oil Board,
43600, Bangi, Selangor, Malaysia
We have previously shown that palm vitamin E accelerates excision
and incision wound closures in diabetic rats when supplemented
at a high dose. In order to establish that palm vitamin E improves
the rates of wound healing, we examined the histological
changes that occur during the process of healing in supplemented
and unsupplemented diabetic rats.
Diabetes mellitus was induced using streptozotocin (50 mg/kg
bw) intramuscularly in 24 fasting male Sprague Dawley rats. Experiment
was started by creating four 6mm diameter wounds on the
back of each animal under sterile conditions using a punch biopsy.
The rats were then supplemented orally with either palm vitamine
E at doses of 5, 50 or 200 mg/kg bw or equivalent volume of olive
oil daily. Wounds were collected ten days after the initial injury,
cross sections were cut and stained with Haematoxylin’s & Eosin
stain and Van Giesons stains.
Results showed that rats supplemented with 200 mg/kg bw PV
had the most proliferation of cells and growth of collagen fibres indicating
improved healing compared to the other groups.
162