oxidants and antioxidants in biology - Oxygen Club of California

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Halliwell B and Gutteridge JMC (1999) Free Radicals in Biology and Medicine. Oxford University Press, New York Remião F, Carvalho M, Carmo H, Carvalho F and Bastos ML (2002) Cu 2+ - Induced isoproterenol oxidation into isoprenochrome in adult rat calciumtolerant cardiomyocytes. Chemical Research in Toxicology 15:861-869 Remião F, Milhazes N, Borges F, Carvalho F, Bastos ML, Lemos-Amado F, Domingues P and Ferrer-Correia A (2003). Synthesis and analysis of aminochromes by HPLC-photodiode array. Adrenochrome evaluation in rat blood. Biomedical Chromatography 17:6-13 176
Applications of flow cytometry in the study of mitochondrial ageing ROYO D, LLORET A, GÓMEZ-CABRERA MC, RUS AD, BORRÁS C, PEREDA J, SASTRE J, PALLARDÓ FV AND VIÑA J Dpt. of Physiology. Faculty of Medicine. University of Valencia. Spain Flow cytometers assay particles in suspension which interfere individually with a beam of light. The intersection of each particle with a laser beam causes the emission of a series of light signals which allow us to separate populations in a given sample by its size, or by its reactivity with various fluorochromes. In our studies mainly mitochondria isolated from Wistar rats young (3 months old), old (24 months) and old treated with antioxidants. Antioxidants, usually Ginkgo biloba, were given in drinking water at a dose of 100 mg per kilo of body weight for 3 months. We have used flow cytometry to study the function of isolated mitochondria from brain and the changes in the process of ageing, both at morphological and functional levels. The following parameters have been measured: mitochondrial membrane potential (Rh123), peroxide levels (Dh123), size, complexity and mitochondrial mass. We find that ageing causes a decrease in mitochondrial membrane potential and a concomitant increase in the rate of oxidant production. Morphologically, we have found two populations of mitochondria of different size and complexity. In old rats, megamitochondria are found. No changes in total mitochondrial mass were found in ageing. All these changes can be diverted by administration of EGb761 extract from Ginkgo biloba. 177
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Oxygen Club of California OXIDANTS
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CONTENTS KEYNOTE ADDRESS...........
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The antioxidant network: From scave
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SESSION I REDOX REGULATION OF CELL
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Bcl-2 expression to promote mitocho
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The zinc-finger domains of kinases
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Proinflammatory and proapoptotic ef
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Novel functions of the mammalian cy
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hypothesize that TBP-2 expression i
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Characteristics of channel formatio
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Mitochondria in nitric oxide-induce
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Mitochondrial nitric oxide synthase
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Mitochondria, proteolysis, calcium,
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Mitochondria and signaling lipids i
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Mitochondrial regulation of apoptot
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NADH-dehydrogenase as a marker of a
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Mitochondrial reactive oxygen speci
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The beneficial effects of fruit pol
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Cell signaling and apoptosis in agi
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Caloric restriction, gene expressio
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Conclusions: Our results have provi
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Selective mesurement of nitric oxid
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Nitric oxide derived oxidants and c
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Nitric oxide (NO) mimics hypoxia by
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We are currently trying to exploit
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Free radicals produced in exhaustiv
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Contraction-induced oxidants as med
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Exercise and NF B Signaling in Rat
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Chlorophyll fluorescence: A reporte
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Role of reactive oxygen species, ni
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Oxidative stress, antioxidants, and
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Action of Vitamin E against Stress
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Metabolism of tocopherols and tocot
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Vitamin E prevents foam cell format
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Tocotrienols: their effect on immun
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Cloning of novel human SEC14p-like
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or absence of LPS, increased NF-κB
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SESSION VIII VITAMIN E AND SELENIUM
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Antioxidant role of selenium: Relev
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Expression of nuclear PHGPx MATILDE
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Carotenoid conversion to Vitamin A:
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Noninvasive laser Raman detection o
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Carotenoids and cancer prevention U
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Cytoprotective and cytotoxic effect
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Flavan-3-ols and procyanidins: beyo
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Effect of genistein and daidzein on
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The antiatherogenic and anti-inflam
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Transcription factor switching by c
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POSTERS
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trols. Their plasma levels of liver
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The reduction of Coenzyme Q 9 at th
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Comparison of antioxidant activitie
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Mn was higher in hepatitis B carrie
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Increased superoxide generation is
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Kidney Mitochondrial Nitric Oxide S
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Thapsigargin induced apotosis ocurr
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Amyloid- and H 2 O 2 induced impair
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Vitamin E recommended daily intake
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Ischemic preconditioning attenuates
Page 125 and 126: Differential zonal distribution of
Page 127 and 128: In vitro antioxidant profile of pol
Page 129 and 130: Oncolyn Neutralizes the Oxidative D
Page 131 and 132: Can insulin have a neuroprotective
Page 133 and 134: Antioxidant defense of Trypanosoma
Page 135 and 136: Evidence for a novel neuroprotectiv
Page 137 and 138: Cell type dependence in NF- B activ
Page 139 and 140: Signals that activate hepatic exerc
Page 141 and 142: Induction of vascular endothelial g
Page 143 and 144: Ascorbic acid decreases uva-induced
Page 145 and 146: Lowering of NO stores in patients w
Page 147 and 148: The possible role of NADP + -depend
Page 149 and 150: the similar kind of influence conce
Page 151 and 152: Molecular study of the peroxisomal
Page 153 and 154: Antioxidant activity of polyphenols
Page 155 and 156: Nitric oxide mediates the up-regula
Page 157 and 158: Antioxidant enzymes in purified chl
Page 159 and 160: Dietary supplementation of essentia
Page 161 and 162: Time-course of rosmarinic acid prod
Page 163 and 164: Moderate exercise increases lifespa
Page 165 and 166: Doxorubicin activates nuclear facto
Page 167 and 168: In vitro antioxidative properties o
Page 169 and 170: Radical scavenging activity of dihy
Page 171 and 172: Enzymatic reduction of glutathione
Page 173 and 174: Biomarker responses in the clam, Sc
Page 175: Analysis of Aminochromes by HPLC-Ph
Page 179 and 180: Key role of xanthine oxidase in wea
Page 181 and 182: Formation of free radicals in the s
Page 183 and 184: The role of oxidative stress and an
Page 185 and 186: Progress towards the discovery of t
Page 187 and 188: Oxidation derived metabolites of -c
Page 189 and 190: The Uptake of Tocopherols by RAW 26
Page 191 and 192: Acute phase immune response to exer
Page 193 and 194: Relationships between antioxidants
Page 195 and 196: Antioxidant activity in extracts fr
Page 197 and 198: Lipid oxidation products with high
Page 199 and 200: Status of antioxidant defense syste
Page 201: Oral administration of Crataegus ex
Page 204 and 205: A Abdelmohsen, K...................
Page 206 and 207: Gómez, M........................63
Page 208 and 209: O Obermueller-Jevic, U.............
Page 210: Yanshu, Z..........................
Page 215: OCC-CÁDIZ FISCAL SOCIETY PRESIDENT
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oxidants and antioxidants in biology - Oxygen Club of California

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