oxidants and antioxidants in biology - Oxygen Club of California
oxidants and antioxidants in biology - Oxygen Club of California
oxidants and antioxidants in biology - Oxygen Club of California
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Oncolyn Neutralizes the Oxidative Damage by Asbestos<br />
<strong>in</strong> vitro, <strong>in</strong> vivo, <strong>and</strong> <strong>in</strong> Patients<br />
ARTHUR H.K. DJANG*, ZHANG YANSHU, YAO LIN,<br />
M. BUD NELSON*<br />
* Santé International, Jamestown, New York 14701, USA<br />
Division <strong>of</strong> Preventive Medic<strong>in</strong>e, North Ch<strong>in</strong>a Coal Medical College, Tangshan<br />
63000 Ch<strong>in</strong>a<br />
Asbestos can cause pulmonary <strong>in</strong>jury, as well as fibrosis <strong>and</strong><br />
mesothelioma; <strong>and</strong> that alveolar macrophages play an important<br />
role <strong>in</strong> these processes. The disorder <strong>of</strong> oxidation <strong>and</strong> antioxidation<br />
may contribute to the process <strong>of</strong> fibrosis <strong>and</strong> mesothelioma caused<br />
by asbestos. Oncolyn® is a formulated extract from three edible<br />
plants conta<strong>in</strong><strong>in</strong>g polyphenols, proanthocyanid<strong>in</strong>s, plant sapon<strong>in</strong>s<br />
<strong>and</strong> other natural plant <strong>in</strong>gredients <strong>in</strong> synergistic comb<strong>in</strong>ation with<br />
effective anti<strong>oxidants</strong> <strong>and</strong> cytoprotective function. The current<br />
study <strong>in</strong>vestigated the effect <strong>of</strong> Oncolyn on the neutralization <strong>of</strong><br />
oxidative damage <strong>in</strong> alveolar macrophages, both <strong>in</strong> vitro <strong>and</strong> <strong>in</strong> rats<br />
<strong>and</strong> used <strong>in</strong> patients with confirmed Mesothelioma. The DNA<br />
str<strong>and</strong> breakage, nitric oxide (NO) generation <strong>and</strong>, superoxide dismutase<br />
(SOD) activity, glutathione peroxidase (GSH-Px) activity,<br />
<strong>and</strong> NO synthesize enzyme were measured <strong>in</strong> alveolar macrophages<br />
treated with asbestos <strong>and</strong> Oncolyn.<br />
In blood <strong>and</strong> lung tissue <strong>of</strong> Wistar rats exposed to asbestos, the<br />
<strong>in</strong>teraction between <strong>in</strong>haled asbestos <strong>and</strong> membranes <strong>of</strong> alveolar<br />
macrophage (AM) is an important event <strong>in</strong> the development <strong>of</strong> fibrosis.<br />
Asbestos can <strong>in</strong>jure alveolar macrophages directly, <strong>and</strong> can<br />
also <strong>in</strong>duce them to release a variety <strong>of</strong> mediators which play a key<br />
role <strong>in</strong> the <strong>in</strong>itial lung <strong>in</strong>jury. Alveolar macrophages obta<strong>in</strong>ed from<br />
Wistar rats cultured with asbestos <strong>and</strong> Oncolyn for one or two<br />
hours. Results from analysis by Comet assay demonstrated that the<br />
percent <strong>of</strong> Comet cells decreased, <strong>and</strong> the length <strong>of</strong> DNA migration<br />
became shorter, <strong>in</strong> alveolar macrophages treated with Oncolyn as<br />
compared to alveolar macrophages receiv<strong>in</strong>g no Oncolyn. Oncolyn<br />
also <strong>in</strong>hibited nitric oxide generation, as measured by the amount <strong>of</strong><br />
nitrite released <strong>in</strong>to the culture medium. NO synthesize enzyme <strong>in</strong><br />
soluble extracts <strong>of</strong> alveolar macrophages treated with Oncolyn for 2<br />
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