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MS20 Laboratory: Physical and Biological Factors Affecting Oxygen ...

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Name__________________ Lab Section_______________ Date________<br />

ANSWER SHEET <strong>Oxygen</strong> Lab<br />

Table Two<br />

Calibration water temp theoretical oxygen starting point ending point mLs of<br />

thiosulfate used<br />

1 st run<br />

2 nd run<br />

Average =<br />

Calculate your unknown concentrations using the average mls of thiosulfate from Table Two <strong>and</strong><br />

the known oxygen concentration from Table One in this equation:<br />

unknown O 2 concentration = known O 2 concentration x mL thiosulfate used for unknown / ml thiosulfate for known<br />

6. Determine the oxygen content of sample 2: 35 o / oo salinity water, room temperature.<br />

milliliters of thiosulfate solution calculate 0 2<br />

7. Determine the oxygen content of sample 3: polluted lake environment.<br />

milliliters of thiosulfate solution calculate 0 2<br />

8. Determine the oxygen content of sample 4: model lake – surface sample.<br />

milliliters of thiosulfate solution calculate 0 2<br />

9. Determine the oxygen content of sample 5: model lake - 75 cm down.<br />

milliliters of thiosulfate solution calculate 0 2<br />

10. Determine the oxygen content of sample 6: model lake - bottom.<br />

milliliters of thiosulfate solution calculate 0 2<br />

Answer the following questions:<br />

1. Assume you misread your buret end point <strong>and</strong> added too much thiosulfate solution for your<br />

st<strong>and</strong>ard. How would this affect all of your other results?<br />

<strong>MS20</strong> Lab <strong>Oxygen</strong> rev.3/27/2005 Page 14 of 16

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