MATRIGENICS.14 G - Cosmesi.it

MATRIGENICS.14 G
Re-activates 14 genes to recover
the Triangle of Beauty in 14 days only

Matrigenics.14 G: extract of fertile bases of Undaria
Matrigenics.14 is a concentrate obtained
from the fertile bases of the alga Undaria
pinnatifida.
This fertile bases, also known has
Mekabu contain the spores that are
responsible for the species’ reproduction
and are considered as a life concentrate,
having precious rejuvenation virtues.
Rich in Wakamic ester, a sulfated
galactofucane this life concentrate reactivates
14 genes implicated in the redensification
of the extra-cellular matrix
of the dermis for a rejuvenating effect.
Wakamic ester

Undaria, Spores and Fertile Bases...
Undaria pinnatifida, from the Latin "unda", a
wave, and pinnatififa, feather-shaped, is also
commonly known as “Wakame” in Japan.
It is a brown macro-algae attaining up to 2m.
When mature, the edges of the stipe become
broader and wavy. These new tissues, known
as fertile bases or even Mekabu, contain the
reproductive cells of the alage.
The Undaria reproductive cells are released
into the environment before the adult plant
dies off at the end of the summer. Each
reproductive cell will then germinate into a
new alga, ensuring the reproduction and the
perennity of the species.
Fertile bases, Mekabu are much appreciated
in Japanese food.
Fertile base, full of
reproductive cells

From Undaria to Matrigenics.14 G
Matrigenics.14 G is an aqueous extract of
fertile bases of Undaria pinnatifida.
In collaboration with local partners, Codif
R&N cultivates the alga in Brittany (France) in
a protected area of the Rance.
• Culture of the alga:
– Initially collected in the sea
– Placed in hatcheries
– Germination, formation of small alga
– Transfer to the open sea for growth
on a longline system
• Harvest:
– Adult algae are collected 1 year after
– Fertile bases are isolated and used
to produce Matrigenics.14 G
– Other parts of the algae are used for
food applications
Cultivation site
Hatcheries
MATRIGENICS.14 G is a natural and
ecofriendly active ingredient.
Longline system

What contributes to the face ageing?
In youth the cheekbones are high and
well defined, and the jaw line is also well
defined, yet soft.
This is called ‘The Triangle of Beauty’.
With ageing appear wrinkles, but also a
descent of the brow, a loss of elasticity
of the skin (jowls develop), a drooping of
the lip edges…
The ‘Triangle of Beauty’ is lost which is
less attractive and make look
permanently unhappy and sad.

What is the main concern of women in terms
of ageing?
A study realized by Arcane Research
(French Poll) on 4400 women in 2011
reveals that:
29% of women are concerned by the
wrinkles between the eyebrows
32% of women are concerned by the
nasal furrows wrinkles
37% of women are concerned by the
lines and furrows around the mouth

What is the role of the ExtraCellular Matrix in the
formation of lines, furrows and wrinkles?
DÉRÈGLEMENT DES ADIPOCYTES ET DES FIBROBLASTES :
UNE ORIGINE GÉNÉTIQUE
The Extracellular Matrix (ECM) is the largest
component of normal skin and gives the skin
its unique properties of elasticity, tensile
strength and compressibility.
These properties are due to the combination
of two main classes of molecules, which are
secreted by fibroblasts and epidermal cells:
• structural proteins: collagens, elastin
• proteoglycans: like hyaluronic acid, which
are large and highly hydrated.
These molecules are assembled and
organized in a specific way into the
Extracellular Matrix in order to ensure the
structural integrity of the skin

What is the role of the ExtraCellular Matrix in the
formation of lines, furrows and wrinkles?
DÉRÈGLEMENT DES ADIPOCYTES ET DES FIBROBLASTES :
UNE ORIGINE GÉNÉTIQUE
The apparition of lines, furrows and wrinkles
is due to a disorganisation of the ECM directly
linked to the quantity and the quality of its
main components: collagen, elastin and
proteoglycan.
The synthesis of these molecules is regulated
by our genes. A gene is a code which orders
the manufacture of a specific protein.
With age, genes are less active and the
quantity and quality of protein synthesised is
lower.
The solution: awaking the activity of genes
responsible for the synthesis of the
components of the ECM.

Action mechanism of Matrigenics.14 G
Goal:
Re-building the Extracellular Matrix (ECM)
Action mechanism:
Re-activation of 14 genes implicated in the
synthesis, the maturation, the assembling
and the anchoring of collagen, elastin and
hyaluronic acid
Forehead wrinkles
Results:
Decrease of forehead wrinkles
Decrease of nasal furrows
Decrease of lines around the lips
Nasal
furrows
Mouth
wrinkles

In-Vitro Test: 2% Matrigenics.14 G stimulates genes implicated in
collagen synthesis, maturation and anchoring.
(2% Matrigenics.14 G, Human Dermal Fibroblasts, Minichips)
Genes involved in the synthesis of collagen fibres
Fibroblast
+15% COL I (Collagen I)
+ 20% COL IV (Collagen IV)
+37% COL VIII (Collagen VIII)
Genes involved in the maturation of collagen fibres
+61% P4HA1 (Proline Hydrolase 1)
+30% P4HA2 (Proline Hydrolase 2)
Genes involved in the assembling of collagen fibres
+50% LOX (Lysyl oxydase)
+43% LOXL2 (Lysyl oxydase L2)
Genes involved in the anchoring of collagen fibres
SYNTHESIS &
MATURATION
ASSEMBLING
ANCHORING
COLs
P4HA1
P4HA2
LOX
LOXL2
LUMICANE
DECORINE
+72% LUMICANE
+23% DECORINE
Dermis Epidermis

In-Vitro Test : Illustration of the Collagen I booster effect of
2% Matrigenics.14 G
Method: Human Dermal Fibroblasts incubated with 2% Matrigenics.14 G for 48 hours. Observation of collagen
fibres using immunolabelling
Without Matrigenics.14 G
Low level of intracellular labelling,
collagen fibres are immature and not assembled
With Matrigenics.14 G
High level of extracellular labelling,
collagen fibres are mature and assembled

In-Vitro Test: 2% Matrigenics.14 G stimulates genes implicated in
elastin synthesis, maturation and anchoring.
(2% Matrigenics.14 G, Human Dermal Fibroblasts, Minichips)
Fibroblast
Genes involved in the synthesis of elastin fibres
+ 21% ELAST (Elastin)
Genes involved in the maturation of elastin fibres
+36% FBN1 (Fibrilline 1)
Genes involved in the assembling of elastin fibres
+50% LOX (Lysyl oxydase)
+43% LOXL2 (Lysyl oxydase L2)
SYNTHESIS &
MATURATION
ASSEMBLING
ELAST
FBN1
LOX
LOXL2
Genes involved in the strengthening and protection of
elastin fibres
+18% TIMP2 (Tissue Inhibitor MetalloProteinase 2)
Dermis Epidermis

In-Vitro Test : Illustration of the Elastin booster effect of
2% Matrigenics.14 G
Method: Human Dermal Fibroblasts incubated with 2% Matrigenics.14 G for 48 hours. Observation of
(Tropo)Elastin fibres using immunolabelling
Without Matrigenics.14 G
Low level of intracellular labelling,
elastin fibres are immature and not assembled
With Matrigenics.14 G
High level of extracellular labelling,
elastin fibres are mature and assembled

In-Vitro Test: 2% Matrigenics.14 G stimulates gene implicated in
Hyaluronic acid synthesis and fibroblast mobility
(2% Matrigenics.14 G, Human Dermal Fibroblasts, Minichips)
Genes implicated in the synthesis of Hyaluronic acid
+68% HAS (Hyaluronic Acid Synthase)
Genes implicated in the mobility of fibroblasts
+31% CD44 (Cluster of Differentiation 44)
Hyaluronic acid
CD44
Fibroblast
HAS
Glucuronic acid + N-
acetyl-glucosamin
CD44 is a transmembraneous proteins which helps
to maintain a balance between Hyaluronic acid
synthesis and degradation into the matrix. In
addition, via CD44, fibroblasts binds hyaluronic acid
to migrate into the dermis, thus ensuring an
homogenous repartition of cells and fibres in this
layer.
Dermis Epidermis

Conclusion on In-Vitro tests
Matrigenics.14 G re-activates 14 genes
Genes linked
to collagen
Genes linked
to elastin
COL1
ELAST
COL2
COL8
P4HA1
P4HA2
LUM
DCN
Genes linked
to collagen &
elastin
FBN1
TIMP2
Genes linked
to hyaluronic
acid
HS
CD44
to restructure the ExtraCellular Matrix
Dermis Epidermis
LOX
LOXL2

Clinical studies
PROTOCOLE
Application:
• Cream containing 2% Matrigenics.14 G
applied twice daily for 14 and 28 days versus
placebo
• 2 groups of 22 volunteers
• Age: 50-65 years
Analysis:
• At T0, T14 days, T28 days
• Analysis of forehead wrinkles, nasal furrows
mouth wrinkles
• Method: innovative analytic method of fringe
projection in 3D.

In-Vivo test: effect on wrinkles and lines of the forehead
2% Matrigenics.14.G
Matrigenics.14 G sigificantly decreases
the lines and wrinkles of the forehead
after 14 days of treatment.
Average and significant decreases :
-23.8%* of the volume of wrinkles
-14.4%* of the area of wrinkles
A prolongation of the treatment to 28
days does not induce a siginificant
variation of volume and area of wrinkles,
compared to D14.
Average variation versus placebo (in %)
0
-5
-10
-15
-20
-25
Volume of wrinkles
-23,8%*
Area of Wrinkle
-14,4%*
*p

In-Vivo test: effect on wrinkles and lines of the forehead
2% Matrigenics.14.G
Visible effect after 14 days of treatment
Before treatment
After 14 days treatment

In-Vivo test: effect on nasal furrows and mouth wrinkles
2% Matrigenics.14.G
Matrigenics.14 G decreases nasal
furrows and mouth wrinklesafter 14 days
of treatment.
Results are improved by prolonging the
treatment to 28 days:
Nasal furrows
-8% of the volume
-9% of the area
Mouth wrinkles
-18% of the volume
-16% of the area
Average variation versus placebo (in %)
0
-2
-4
-6
-8
-10
-12
-14
-16
-18
-20
Nasal furrows
Mouth wrinkles
Volume Area Volume Area
-2%
-3%
-4%
-6%
-8%
-9%
-16%
-18%
14 days 28 days

In-Vivo test: 2% Matrigenics.14.G
Visualisation of the anti-wrinkle effect on the nasal furrows and
mouth wrinkle
Visible effect after 14 days of treatment
Before treatment
After 14 days of treatment
Nasal furrows and mouth wrinkles are visibly reduced

In-Vivo test: 2% Matrigenics.14.G
Visualisation of the global anti-aging effect
Both depth and length of nasal
furrows are visibly reduced
Before
After
Before
After
The forehead is smoother and the
negative expression of the mouth
is soften

Conclusion on Matrigenics.14 G
Concentrate of fertile bases of the alga Undaria pinnatifida
Rich in Wakamic ester
Cosmetic Effects
Re-activation of 14 genes implicated in:
- collagen synthesis and anchoring
- elastin synthesis and anchoring
- hyaluronic acid synthesis and fibroblasts mobility
Clinical results after 14 days only on:
- volume and area of the wrinkles between the
eyebrows
- volume and area of the nasal furrows
- volume and area of the mouth wrinkles
Fights against the inversion of the Triangle of Beauty
INCI Name (compliant with Chinese regulation)
Glycerin (and) Water (and) Undaria pinnatifida extract
Recommended use: 2%