Hematopathology Resident / Fellow Manual - Department of ...

4. Flow Cytometry Laboratory Experience (2½ days)
Understanding of flow cytometric immunophenotypic techniques and interpretation of the
resultant data is an integral part of all the bone marrow and lymph node rotations. In
addition, however there is a brief concentrated exposure to the flow cytometry laboratory
including the technical aspects of flow cytometry and the basic operation of a flow
cytometry laboratory. The resident should report to Karen Freilino or their designate.
Goals and Objectives:
1. Understand sample preparation; basic flow cytometry, quality control, gating on
specific cell populations, and determination of positive versus negative staining
and methods of data presentation.
2. Know indications for testing, taking into account cost effective medicine
3. Complete flow cytometry checklist.
5. Adult Bone Marrow Experience (~ 4 weeks)
A. Limited Sessions with Adult Bone Marrow Technologist.
B. Responsibility for review of selected cases prior to sign-out.
C. Participation in sign-out with staff.
D. Review of educational materials (See Adult Bone Marrow Experience section).
Goals and Objectives:
1. Learn normal and abnormal blood cell morphology.
2. Learn basic approach to bone marrow aspirate, biopsy and particle preparation
interpretation.
3. Begin to become familiar with the use of ancillary studies used in the diagnosis of
bone marrow examinations.
4. Begin to develop a basic understanding of the more common neoplastic and nonneoplastic
disorders which involve the marrow: acute and chronic leukemias,
myelodysplasias, myeloproliferative disorders, anemias, thrombocytopenias,
leukopenias, thrombocytosis, leukocytosis, infections (including HIV), and
metastatic neoplasms. Be able to diagnose straightforward cases of the above.
5. Complete bone marrow rotation checklist.