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366 Manual of basic techniques for a health laboratory Transfer the stock solution to a brown bottle. Label the bottle “THIOMERSAL– FORMALDEHYDE STOCK SOLUTION” and write the date. The stock solution will keep for up to 3 months. Warning: Formaldehyde is corrosive and poisonous. 2. On the day of use, mix: Stock thiomersal solution 9.4ml Lugol iodine, 50g/l (5%) solution (No. 37) 0.6ml Trisodium citrate, 20g/l (2%) solution in saline (No. 59) Trisodium citrate, dihydrate (Na 3 C 6 H 5 O 7·2H 2 O) 2 g Sodium chloride, 8.5g/l (0.85%) solution (No. 53) q.s. 100ml Keep in the refrigerator. Label the volumetric flask “TRISODIUM CITRATE 2% SOLUTION IN SALINE” and write the date. Trisodium citrate, 32g/l (3.2%) solution (No. 60) This is used as an anticoagulant. Trisodium citrate, anhydrous (Na 3 C 6 H 5 O 7 ) 3.2g (or an equivalent quantity of either the dihydrate or the pentahydrate) Distilled water q.s. 100ml Keep in the refrigerator. Use 1ml of the solution per 4ml of blood. Label the volumetric flask “TRISODIUM CITRATE 3.2% SOLUTION” and write the date. Türk solution (No. 61) Glacial acetic acid (CH 3 COOH) 4 ml Methylene blue solution (No. 39) 10 drops Distilled water q.s. 200ml Dissolve the glacial acetic acid in 100ml of the distilled water. Add the methylene blue solution and mix. Transfer the mixture to a 200-ml volumetric flask and make up the volume to 200ml with distilled water. Label the volumetric flask “TURK SOLUTION” and write the date. Warning: Glacial acetic acid is highly corrosive. Urea reagents (No. 62) Trichloroacetic acid, 50g/l (5%) solution Trichloroacetic acid (CCl 3 COOH) 10g Distilled water q.s. 200ml Weigh the acid out quickly; it is highly deliquescent. Transfer to a beaker. Add 100ml of distilled water and mix to dissolve the chemical. Transfer the solution to a 200-ml volumetric flask and make up the volume to 200ml with distilled water. Label the volumetric flask “TRICHLOROACETIC ACID 5% SOLUTION” and write the date. Warning: Trichloroacetic acid is highly corrosive.
Annex. Reagents and their preparation 367 Diacetyl monoxime stock solution Diacetyl monoxime (also called 2,3-butanedione monoxime) 2 g Distilled water q.s. 500ml Label the volumetric flask “DIACETYL MONOXIME STOCK SOLUTION” and write the date. The solution will keep for at least 6 months at 2–8 °C. Colour reagent Acid reagent (No. 6) 50ml Diacetyl monoxime reagent 50ml Mix the acid reagent and stock solution in a 100-ml stoppered flask. Label the flask “COLOUR REAGENT”. The quantities shown above are sufficient for 33 measurements. The reagent must be prepared daily. Urea stock reference solution, 125mmol/l Urea 750mg Benzoic acid, 1g/l (0.1%) solution (see No. 30) q.s. 100ml Dissolve the urea in about 20ml of the benzoic acid solution in a 100-ml volumetric flask. Make up the volume to 100ml with benzoic acid solution. Label the flask “UREA STOCK REFERENCE SOLUTION 125 MMOL/L” and write the date. Store in a refrigerator. The solution will keep for several months at 2–8 °C. Urea working reference solution, 10mmol/l Urea stock reference solution 8 ml Benzoic acid (C 7 H 6 O 2 ), 1g/l (0.1%) solution (see No. 30) q.s. 100ml Mix the solutions well in a 100-ml volumetric flask. Label the volumetric flask “UREA WORKING REFERENCE SOLUTION 10 MMOL/L” and write the date. Wayson stain (No. 63) Solution A 1 : Basic fuchsin 2 g Absolute methanol (CH 3 OH) 100ml Solution A 2 : Methylene blue 7 g Absolute methanol (CH 3 OH) 100ml Combine the two solutions to give solution A. Solution B (phenol, 50g/l (5%) solution): Phenol (C 6 H 5 OH) 100g Distilled water 2000ml Add solution A to solution B. The staining properties of Wayson stain improve with age. Make the stain in large quantities and dispense it in small amounts in to dark bottles for future use. Label the bottles “WAYSON STAIN” and write the date. Warning: Phenol is corrosive.
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M A N U A L O F B A S I C TECHNIQUE
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Contents i Manual of basic techniqu
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Contents iii Contents Preface x 1.
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Contents v 4.2.1 Collection of spec
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Contents vii PART III 231 7. Examin
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Contents ix 10.1.3 Method 322 10.1.
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1. Introduction 1 1. Introduction 1
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1. Introduction 3 The following sec
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1. Introduction 5 Table 1.4 SI deri
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1. Introduction 7 Table 1.5 (cont.)
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2. Setting up a peripheral health l
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3. General laboratory procedures 53
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4. Parasitology 103 Part II
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4. Parasitology 105 4.Parasitology
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4. Parasitology 107 4.2 Examination
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4. Parasitology 109 colourless, red
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4. Parasitology 111 Table 4.3 Patho
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4. Parasitology 113 Fig. 4.13 Invas
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4. Parasitology 115 Motility: eithe
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4. Parasitology 117 Rapid Field sta
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4. Parasitology 119 Fig. 4.24 Featu
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4. Parasitology 121 Identification
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4. Parasitology 123 Measurement Acc
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4. Parasitology 125 Shape: round or
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4. Parasitology 127 ● ● ● The
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4. Parasitology 129 Fig. 4.39 Terms
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4. Parasitology 131
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4. Parasitology 133 Fig. 4.42 Ancyl
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4. Parasitology 135 Dipylidium cani
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4. Parasitology 137 Fig. 4.59 Trans
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4. Parasitology 139 Fig. 4.66 Heter
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4. Parasitology 141 Shell: smooth,
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4. Parasitology 143 Fig. 4.79 Filli
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4. Parasitology 145 These granules
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4. Parasitology 147 Fig. 4.92 Adult
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4. Parasitology 149 Fig. 4.97 Featu
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4. Parasitology 151 Fig. 4.101 Schi
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4. Parasitology 153 Method Preparat
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4. Parasitology 155 2. Using the sp
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4. Parasitology 157 differentiated
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4. Parasitology 159 5. Add to the t
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4. Parasitology 161 Method Collecti
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4. Parasitology 163 Procedure for o
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4. Parasitology 165 Table 4.8 Chara
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4. Parasitology 167 Method 1. Colle
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4. Parasitology 169 7. Remove the s
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4. Parasitology 171 Cephalic space
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4. Parasitology 173 Table 4.10 Geog
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4. Parasitology 175 Fig. 4.132 Coll
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4. Parasitology 177 1. Allow the th
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4. Parasitology 179 Table 4.11 Comp
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4. Parasitology 181 Table 4.12 (con
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4. Parasitology 183 African trypano
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4. Parasitology 185 Fig. 4.144 Twis
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4. Parasitology 187 Fig. 4.150 Usin
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4. Parasitology 189 The above mater
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4. Parasitology 191 Fig. 4.158 Appl
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4. Parasitology 193 clinical sympto
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4. Parasitology 195 Examination of
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5. Bacteriology 197 5. Bacteriology
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5. Bacteriology 199 5.2.4 Fixation
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5. Bacteriology 201 Fig. 5.14 “Ac
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5. Bacteriology 203 Table 5.2 Repor
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5. Bacteriology 205 ● Parasites:
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5. Bacteriology 207 Throat specimen
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5. Bacteriology 209 Other bacteria
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5. Bacteriology 211 5.6.3 Microscop
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5. Bacteriology 213 Fig. 5.28 Sperm
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5. Bacteriology 215 3. Load an impr
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5. Bacteriology 217 Fig. 5.34 Vibri
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5. Bacteriology 219 2. Remove the s
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5. Bacteriology 221 5.12.2 Method C
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5. Bacteriology 223 Fig. 5.43 Trans
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6. Mycology 1 6.1 Examination of sk
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6. Mycology 227 6.2.1 Materials and
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6. Mycology 229 Fig. 6.8 Transferri
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232 Manual of basic techniques for
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Page 382 and 383: 370 Index BI, see Bacteriological i
Page 384 and 385: 372 Index Dark-field microscopy 64,
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