Dutch Boltz - Molecular Devices

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Inhibition of Membrane Depolarization by Compounds A and B Compound A IC 50 = 0.074µM IC 50 = 0.066µM Compound B IC 50 = 0.35µM IC 50 = 0.74µM FLIPR FR IPR 6/14/2004 Cardiovascular Diseases 16
Inhibition of Membrane Depolarization by Compounds C and D Compound C IC 50 = 0.13µM IC 50 = 0.31µM Compound D IC 50 = 0.17µM IC 50 = 0.45µM FLIPR FR IPR 6/14/2004 Cardiovascular Diseases 17
Page 1 and 2: Dutch Boltz • Dutch has been at M
Page 3 and 4: Today’s s Presentation • Review
Page 5 and 6: Voltage Dependent FRET Mechanism of
Page 7 and 8: Comparison of the Two “I” Plate
Page 9 and 10: Bleed Down of “FR IPR” Membrane
Page 11 and 12: Components Used To Modify FLIPR to
Page 13 and 14: FRET Dye Protocol • 30,000 HEK-2
Page 15: 384 Well FLIPR Ratio Traces for a 1
Page 19 and 20: Areas for Improvement • Can Optic
Page 21 and 22: For More Details and Insight See Ri
Page 23 and 24: Abstract Ion channels are an attrac
Page 25 and 26: Coumarin-Donor Oxonol-Acceptor Fluo

Dutch Boltz - Molecular Devices

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