Dutch Boltz - Molecular Devices

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Conclusion of 488 nm/409 nm FLIPR FRET Membrane Potential Application Study • An Additional Laser Beam Can be Combined into The FLIPR 384 Optics • Original Operation “Unaffected” • Coumarin DiSBAC2 FRET Data Can Be Collected • FLIPR Eliminates Signal Bleed Down • Excellent Titration Data Correlation Between Plate Readers Tested • IC 50s Consistently Lower • The System is Robust and Yields Reproducible Data • Kinetics Appeared to be Faster Than <strong>Molecular</strong> <strong>Devices</strong> Red Dye > 2 Minutes 6/14/2004 Cardiovascular Diseases 20
For More Details and Insight See Rich Wnek’s Poster 6/14/2004 Cardiovascular Diseases 21
Page 1 and 2: Dutch Boltz • Dutch has been at M
Page 3 and 4: Today’s s Presentation • Review
Page 5 and 6: Voltage Dependent FRET Mechanism of
Page 7 and 8: Comparison of the Two “I” Plate
Page 9 and 10: Bleed Down of “FR IPR” Membrane
Page 11 and 12: Components Used To Modify FLIPR to
Page 13 and 14: FRET Dye Protocol • 30,000 HEK-2
Page 15 and 16: 384 Well FLIPR Ratio Traces for a 1
Page 17 and 18: Inhibition of Membrane Depolarizati
Page 19: Areas for Improvement • Can Optic
Page 23 and 24: Abstract Ion channels are an attrac
Page 25 and 26: Coumarin-Donor Oxonol-Acceptor Fluo

Dutch Boltz - Molecular Devices

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