Dutch Boltz - Molecular Devices

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Why Combine FLIPR and FRET Dye? • Utilize Optimal Parts of Two Approaches • Increased Sensitivity? on FLIPR • Increased Reliability? • Increased Reproducibility ? • Greater Throughput for Longer Time Courses > 1-21 2 minutes • Standardization between Screening Platforms • Increase Throughput- Multiple Instrument Campaign 6/14/2004 Cardiovascular Diseases 6
Comparison of the Two “I” Plate Readers “IPR”s Micro titer Well PMT PMT 2 FL Slider Micro titer Well Cooled CCD 6/14/2004 Cardiovascular Diseases 7
Page 1 and 2: Dutch Boltz • Dutch has been at M
Page 3 and 4: Today’s s Presentation • Review
Page 5: Voltage Dependent FRET Mechanism of
Page 9 and 10: Bleed Down of “FR IPR” Membrane
Page 11 and 12: Components Used To Modify FLIPR to
Page 13 and 14: FRET Dye Protocol • 30,000 HEK-2
Page 15 and 16: 384 Well FLIPR Ratio Traces for a 1
Page 17 and 18: Inhibition of Membrane Depolarizati
Page 19 and 20: Areas for Improvement • Can Optic
Page 21 and 22: For More Details and Insight See Ri
Page 23 and 24: Abstract Ion channels are an attrac
Page 25 and 26: Coumarin-Donor Oxonol-Acceptor Fluo

Dutch Boltz - Molecular Devices

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