Dutch Boltz - Molecular Devices

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IPR Design Criteria FLIPR • Ion Channel Screening • Membrane Potential Dye • High Throughput • Kinetic Measurement • Simultaneous • Whole Plate • Temperature Control • Biology • Fluorescent Signal • 488 nm Excitation “FR-IPR” • Ion Channel Screening • More Sensitive S/N • PMT • More Rapid Kinetics • < 20 msec • Partial Plate PMT • “Membrane/Potential” FRET Pair • Multi Wavelength (409nm) 6/14/2004 Cardiovascular Diseases 8
Bleed Down of “FR IPR” Membrane Potential Signal Over Time 16 Well Addition/Detection Array Negative Controls Well Acquisition Time Order 6/14/2004 Cardiovascular Diseases 9
Page 1 and 2: Dutch Boltz • Dutch has been at M
Page 3 and 4: Today’s s Presentation • Review
Page 5 and 6: Voltage Dependent FRET Mechanism of
Page 7: Comparison of the Two “I” Plate
Page 11 and 12: Components Used To Modify FLIPR to
Page 13 and 14: FRET Dye Protocol • 30,000 HEK-2
Page 15 and 16: 384 Well FLIPR Ratio Traces for a 1
Page 17 and 18: Inhibition of Membrane Depolarizati
Page 19 and 20: Areas for Improvement • Can Optic
Page 21 and 22: For More Details and Insight See Ri
Page 23 and 24: Abstract Ion channels are an attrac
Page 25 and 26: Coumarin-Donor Oxonol-Acceptor Fluo

Dutch Boltz - Molecular Devices

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