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Dutch Boltz - Molecular Devices

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Conclusion of 488 nm/409 nm FLIPR FRET<br />

Membrane Potential Application Study<br />

• An Additional Laser Beam Can be Combined into The FLIPR 384 Optics<br />

• Original Operation “Unaffected”<br />

• Coumarin DiSBAC2 FRET Data Can Be Collected<br />

• FLIPR Eliminates Signal Bleed Down<br />

• Excellent Titration Data Correlation Between Plate Readers Tested<br />

• IC 50s Consistently Lower<br />

• The System is Robust and Yields Reproducible Data<br />

• Kinetics Appeared to be Faster Than <strong>Molecular</strong> <strong>Devices</strong> Red Dye > 2<br />

Minutes<br />

6/14/2004 Cardiovascular Diseases 20

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