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annual report - Central Institute of Brackishwater Aquaculture

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41.5696, fat 8.13% carbohydrate 21.9746,<br />

moisture 53%, crude fibre 4.46% and ash<br />

1854%) was supplied in 4 grades namely<br />

Pre-starter (0.5 mm), Starter (1.0 mm),<br />

Grower and Finisher (3 mm). At a stocking<br />

density <strong>of</strong> 70,000 noha, P. monodon (PL20)<br />

attained an average size <strong>of</strong> 22 g in 112 days,<br />

the growth being comparable to that achieved<br />

with imported mmmercial feeds.<br />

Production and testing <strong>of</strong> shrimp feeds in<br />

interaction with industry<br />

In order to promote interaction with<br />

the shrimp industry for on-farm research,<br />

steps have been initiated to collaborate with<br />

the commercial feed manufacturers for<br />

large-scale production <strong>of</strong> feeds wing the feed<br />

formulations developed by the <strong>Institute</strong>.<br />

'Testing <strong>of</strong> these feeds under field conditions<br />

in association with the industry will be subsequently<br />

taken up.<br />

Development <strong>of</strong> standards for quality<br />

control <strong>of</strong> shrimp feeds<br />

To develop quality standards for<br />

shrimp feeds, fifteen commercial feeds (both<br />

indigenous and imported) were ana1yst.d for<br />

their proximate composition. Indigenous<br />

feeds generally showed higher percentag ol<br />

acid insoluble ash compared to imp~rted<br />

feeds. Moisture content <strong>of</strong> some <strong>of</strong> the feeds<br />

analy.sed was high (17%). Water stability <strong>of</strong><br />

pellets determined at different time intervals<br />

indicated that loss in weight <strong>of</strong> pellets occurred<br />

in the first half-hour and almost remained<br />

constant upto seven hours. Studies<br />

are in progress using standard AOAC methods<br />

to estimate the total volatile nitrogen<br />

which determines the rancidity <strong>of</strong> fat for<br />

stored feed samples.<br />

Diagnosis, prophylaxis and control <strong>of</strong> die<br />

eases <strong>of</strong> commexcially impottant brackish<br />

water shrimp/fish (TIDPISP)<br />

Madras : S. V. Alavandi (PL), T.C.<br />

Santiago, K. K. Vijayan, S. S. Mishra, K. V.<br />

Rajendran, M.Shashi Shekhar and K.O.<br />

Joseph.<br />

Shrimp farms in the coastal districts <strong>of</strong><br />

Orissa vu., Bhadrak and Balasnre and Andhra<br />

Pradesh vu., East Godavari, West Godavari,<br />

Krishna and Prakasam were surveyed<br />

to investigate the shrimpdisease outbreaksin<br />

those areas. Detalled microbiological and<br />

histopathological investigations revealed<br />

that the white spot disease was the major<br />

cause <strong>of</strong> mortality <strong>of</strong> shrimpP. monodon and<br />

1'. indrcus in most <strong>of</strong> the farms. Analysis <strong>of</strong> P.<br />

rnonodon samples from shrimp farms near<br />

Kannur (Kerala) revealed that the disease<br />

had also struck the coastal districts along the<br />

west coast. Further, the incidence <strong>of</strong> white<br />

spot disease was also ~hserved in the brtx>dstock<br />

ol IJ. rnutzdon in shrimp hatchcries at<br />

Madras and Nellore. 'The causative agent <strong>of</strong><br />

this disease was idcntificd to hc a virus, the<br />

Systemic Eetodermal and Mesodermal Uaculovirus<br />

(SEMRV). Ihe major clinical ymptoms<br />

included circumscrikd white spots on<br />

the carapace and other parts <strong>of</strong> the cuticle,<br />

broken antennae and discolourntion <strong>of</strong> the<br />

hody. Experimental induction <strong>of</strong> the disease<br />

to study the host range <strong>of</strong> the virus revealed<br />

that in addition to shrimp, crabs<strong>of</strong> the genus<br />

SryUu were also affected by the disease.<br />

Studies on the mode <strong>of</strong> transmission <strong>of</strong> the<br />

virus lo the host indicated that SliMDV infection<br />

can k elicited in healthy animals by<br />

feeding on the carcass <strong>of</strong> infected animals as<br />

well as by intra-muscular injection with extracts<br />

<strong>of</strong> gills from the same.<br />

Apart from the while spot disease,<br />

Monodon Bnculovirus (MBV) was also<br />

prevnlent in the farms in Andhra I'radesh and<br />

Tamil Nadu. The shrimp larvae were affected<br />

by this diwasc in some or the hatcheries<br />

'n Nellorc and Ongole (A.P) and<br />

Kovllam and Chinglepei ireas (T.N.). In<br />

addition lo these two major diseases, Vibriosis<br />

and protozoan infection cauwd by<br />

Zoothrrmnium sp, were also common in both<br />

hatcheries and farms.

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