N =120 40-- [_ M 30 - - @ F _ 54.1% r f, 2010- - 10% ,-,.,"" ,,-,'"""": _jj ¢1a4, _35.8'_ , • r/J C P CL CL+CP alone alone Fig. 2 I. Family H. (+) II. Maternal H. '_--_-----"-- _"_-E__:_e_.,_ , - 57% Illness I 1.7% j, Trauma 12.7% Medications _ 6.3% Ill.F.H. or M.H.'(-) _19.8% 10 20 30 40 50 60 Fig. 3 57
UNIVERSITY PHILIPPINE DEPARTMENT OF THE PHILIPPINES GENERAL HOSPITAL OF OTOLARYNGOLOGY TITLE:MICROBIALFLORAIN CHRONICOTITISMEDIA:VALUEOFEARASPIRATE CULTURESTUDIES AUTHORS:Rhodoradel Rosario,MD CharlotteM. Chiong,MD AbnerL. Chan,MD EditaC. Yap, MD JoselitoC. Jamir,MD GenerosoT. Abes,MD ABSTRACT A comparative study was done to compare the , microbiology of the ear aspirate to the contents in the Culture and sensitivity studies on ear aspirate, middle ear cleft namely, cholesteatoma, bone and cholesteatoma, bone and granulation tissue were granulation tissue. The organisms were likewise done on 30 patients with chronic otitis media who identified andthe sensitivity pattern of the commonly underwent radical mastoidectomy. The most com- isolated organisms were determined. mon organisms isolated were Proteus mirabilis,Proteus vulgaris and Pseudomonas aeruginosa. All three showed a 100% sensitivity to ampicillin, trimethoprim, MATERIALS AND METHODS chloramphenicol and cephalexin, Bacteroides, an anaerobic organisms, was grown in only 4/100 Thirty patients with chronic otitis media, 20 males isolates. Cultures from the transcanal aspirates were and ten females with age range 6-45 years (Table 1) compared to those obtained directly from the middle seen at the ENT Dept. who underwent radical masear. Computation of the positive predictive value toidectomy were included in the study. Culture showed that the transcanal aspirate showed a good and sensitivity studies were done on ear aspirate, excellent correlation for the three most common cholesteatoma, bone and granulation tissue specimens. organisms cultured. Ear discharge was aspirated aseptically after the ear canal was swabbed with aloohol soaked cotton. The INTRODUCTION other specimens were collected intraoperatively. All _ were immediately placed in thioglycolate media. These Chronic otitis media (COM) is a common problem were then inoculated in blood agar plate (BAP), and encountered in the ENT clinic. It is an insidious Mac Conkey agar plate. Incubation at 37 C for 24 and potentially destructive disease characterized by hours was done after which the plates were read. the presence of intractable mucosal, submucosal and/ Colonies were identified by their morphologic and or bone pathology of the middle cleft. In this biochemical characteristics. Antibiotic susceptabilinstitution, 60% of 269 ears operated on during the ity of the aerobic isolates to various drugs were year 1989 were for chronically discharging ears. Inspite tested using standard Kirby-Bauer method. For the of the prevalence of the disease, antibiotic treatment anaerobic culture, the specimen from the thioglycote is still largely empirical as a culture and sensitivity medium were inoculated in a fresh blood agar plate studies are not routinely done. One method of under gas Pak method. If the isolates were the same obtaining specimen for culture in the clinic is by as those found in the aerobic culture, no further transcanal aspiration of ear discharge. However, it susceptibility studies were done. However if the is yet to be determined whether this method would colonies identified were the same as those found in be truly reflective of the microbial population of the aerobic culture, no further susceptibility studies middle ear. were done. However if the colonies identified were 58