Elena Cattaneo, PhD - CusMiBio
Elena Cattaneo, PhD - CusMiBio
Elena Cattaneo, PhD - CusMiBio
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<strong>Elena</strong> <strong>Cattaneo</strong>, <strong>PhD</strong><br />
Department of Pharmacological Sciences and<br />
Centre for Stem Cell Research<br />
University of Milano<br />
www.cattaneolab.it
Stem Cell Applications<br />
Donated<br />
blastocysts<br />
Donated<br />
tissues<br />
Stem cell<br />
biology<br />
Nerves<br />
Stem Cell<br />
Factor<br />
Blood<br />
Heart<br />
Modelling<br />
diseases<br />
Embryonic<br />
stem cells<br />
Adult<br />
(tissue derived)<br />
stem cells<br />
Muscle<br />
Drug discovery<br />
Transplantation
In search for<br />
novel neural stem cell lines
ES Neural induction Differentiation<br />
NS<br />
NS<br />
NS<br />
NS<br />
Serum free media<br />
for stable, long-term<br />
expansion in monolayer<br />
Conti and Pollard et al., PLoS Biol. 2005
ES<br />
Neural induction<br />
Set of markers for<br />
Embryonic Neurogenic<br />
Radial Glia<br />
NS<br />
NS<br />
NS<br />
NS<br />
Nestin+<br />
Vimentin+<br />
RC2+ Sox1-<br />
Emx+ Nanog-<br />
Sox2+ Oct4-<br />
BLBP+ TuJ1-<br />
Pax6+ GFAPplus<br />
array data<br />
Differentiation
Up to now NS cells have been<br />
propagated<br />
for 170 passages
Sources of NS cells<br />
Blastocyst<br />
ES<br />
cells<br />
Fetal<br />
brain<br />
Adult<br />
Brain<br />
(SVZ)<br />
NS cells
How about their differentiation
Glial differentiation<br />
ES<br />
Neural induction<br />
NS<br />
NS<br />
NS<br />
NS<br />
Differentiation
Neuronal differentiation<br />
ES<br />
Neural induction<br />
NS<br />
Differentiation<br />
NS<br />
NS<br />
NS
No tumor formation in<br />
approx 150 transplanted animals
Cell replacement<br />
NS cells<br />
Gene function studies<br />
Disease models and<br />
drug screening
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