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High Power Diode Lasers - Center for Biomedical Optics and New ...

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CeramOptec GmbH<br />

Application Test of 732 nm system I<br />

Evaluation of photo-toxicity of PDT substance (BLC2025)<br />

Typical test procedure<br />

• Growth of cell cultures<br />

• Incubation with photosensitizer in<br />

varying concentrations<br />

• Excitation of the sensitizer with<br />

different energy densities <strong>and</strong><br />

total energy<br />

• Continuation of cell growth<br />

• Determination of survival rate,<br />

e.g. via concentration of<br />

enzymes, which only exist in<br />

living cells<br />

100µl cell suspension per well

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