High Power Diode Lasers - Center for Biomedical Optics and New ...
High Power Diode Lasers - Center for Biomedical Optics and New ...
High Power Diode Lasers - Center for Biomedical Optics and New ...
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CeramOptec GmbH<br />
Application Test of 732 nm system I<br />
Evaluation of photo-toxicity of PDT substance (BLC2025)<br />
Typical test procedure<br />
• Growth of cell cultures<br />
• Incubation with photosensitizer in<br />
varying concentrations<br />
• Excitation of the sensitizer with<br />
different energy densities <strong>and</strong><br />
total energy<br />
• Continuation of cell growth<br />
• Determination of survival rate,<br />
e.g. via concentration of<br />
enzymes, which only exist in<br />
living cells<br />
100µl cell suspension per well