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Zeiss Libra 120 en_brief_V10.vp

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20. Adjust Spot<br />

• Increase magnification to 100k with knob (3)<br />

• Switch to Spot mode with button (20).<br />

• Increase spot size to 100 nm with knob (5).<br />

• Push Cal (25).<br />

• Shift spot to the index point with knobs (1, 6).<br />

M4<br />

M3<br />

M2<br />

M1<br />

20<br />

25<br />

5<br />

43<br />

30<br />

31<br />

1 6<br />

• Rec<strong>en</strong>ter spot to index point with knobs (1, 6).<br />

• Switch back to TEM mode with button (20)..<br />

21. C<strong>en</strong>ter Cond<strong>en</strong>ser Aperture<br />

• Click on AIS Off in the tab sheet TEM.<br />

− Display in the status bar.<br />

• C<strong>en</strong>ter cond<strong>en</strong>ser aperture (37.5 µm) to the<br />

large scre<strong>en</strong> mechanically.<br />

22. Insert Specim<strong>en</strong> Holder<br />

• Insert objective aperture (1. clickstop).<br />

− see point 25 for adjustm<strong>en</strong>t.<br />

• Lower mag to 50 k.<br />

• Move holder back into the column and look<br />

for a specim<strong>en</strong> detail.<br />

• C<strong>en</strong>ter detail to the index point.<br />

23. Adjust Euc<strong>en</strong>tric Height<br />

• Switch on Foc Aid with button (26).<br />

• Calibrate the objective l<strong>en</strong>s with button (25).<br />

− The sample is moving forth and back, if it is<br />

not in the euc<strong>en</strong>tric plane.<br />

• Adjust Z with the focus knob (4).<br />

M4<br />

M3<br />

M2<br />

M1<br />

25<br />

22<br />

26<br />

4<br />

5<br />

• Turn off Foc Aid with button (26).<br />

6

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