HPLC and UHPLC Columns

Will the column provide reproducible chromatography from columnto-column
and batch-to-batch
ACE columns have a well-deserved reputation for reproducibility.
Column-to-column reproducibility is affected by the production of the
silica stationary phase support, the bonding of the stationary phase to
the stationary phase support, and the packing of the stationary phase
into the column. The better each stage of the column manufacturing
process is controlled, the better will be the reproducibility and the
quality of the column.
ACE columns are subjected to extensive controls at each stage of
the column manufacturing process. These controls ensure that
ACE columns produce reliable, predictable performance from
column-to-column and lot-to-lot. Figure 13 provides an example of
a typical Batch Validation test.
Subtle changes in silanol activity are one of the primary causes of
column-to-column selectivity changes. ACE and ACE Excel columns
have excellent reproducibility due to the use of ultra pure reagents
and strict control of the manufacturing process that results in a
high purity silica stationary phase support with uniform surface
characteristics. Combining this high purity silica with advanced
bonding techniques results in a family of highly inert phases that
provide an outstanding level of column-to-column reproducibility by
virtually eliminating silanol interference in the chromatography.
Figure 13: Example of a batch validation report for an ACE column
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