HPLC and UHPLC Columns

Figure 30: Catecholamines
Conditions
Column: ACE 5 AQ, 150 x 4.6mm
Mobile Phase: (a) 50mM KH 2
PO 4
, (pH 3.0)
(b) 50mM KH 2
PO 4
, (pH 2.0)
(c) 0.1% TFA
(d) 0.1% HCO 2
H
Flow Rate: 1.0ml/min
Temperature: Ambient
Detection: UV, 210nm
Injection Volume: 2μl
Compounds
1. Noradrenaline
2. Adrenaline
3. L-DOPA
4. Dopamine
5. L-Tyrosine
6. VMA (vanillylmandelic acid)
Response–MilliVolts
Response–MilliVolts
350
(a) 50mM KH 2
PO 4
, (pH 3.0) 250
(c) 0.1% TFA
300
2
200
2
250
150
200
1
4
1
150
100
4
100
3
6
50
3
50
5
6
5
0
0
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
0 2 4 6 8 10 12 14 16 18 20
Time (min)
Time (min)
350
300
(b) 50mM KH 2
PO 4
, (pH 2.0)
350
300
(d) 0.1% HCO 2
H
2
2
250
200
250
200
4
4
1
150
150 1
100
100
3
3
6
50
50
5
5
6
0
0
Response–MilliVolts Response–MilliVolts
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Time (min)
Time (min)
25