Report 2008-2010

DNA Sequencing Center
Eglė Rudokienė, M.Sc.
Rimantas Šiekštelė, M.Sc.
phone: 370 5 2691883
e-mail: egru@ibt.lt; sieksta@ibt.lt
DNA Sequencing Center (SC) of the Institute of
Biotechnology (IBT) is successfully running since
March 27 of 2003. SC was founded to help researchers,
both at IBT as well as other institutions
in Lithuania, process DNA samples in an efficient
and economical manner. The Center is equipped
with the Applied Biosystems 3130xl Genetic Analyzer
16-capillary automated DNA sequencer
that yields 700 to 1000 bases per template. It performs
cycle sequencing reactions using fluorescent
dye terminators ABI Big Dye® Terminator
v3.1 on any kind of DNA (plasmid, phage or PCR
product) provided by the users. We also run the
user's reactions. Usually, turnaround time takes
2-3 days after the receipt of samples. Sequencing
of the larger samples may take longer.
Services provided by the DNA SC include:
* Custom DNA Sequencing
* Sequencing, PCR troubleshooting and
training workshops
We are committed to giving every user
satisfactory sequence.
Number of DNA sequencing reactions performed
by SC for the Lithuanian institutions in 2008 - 2010
Institute of Biotechnology 8122
Vilnius University Hospital Santariskiu Klinikos 5130
Institute of Biochemistry 4232
Institute of Ecology of Vilnius University 1961
Vilnius University 1067
Lithuanian Institute of Agriculture 300
Veterinary Institute of Lithuanian Veterinary Academy 265
Vytautas Magnus University 143
UAB Fermentas 79
State Plant Protection Service 76
Kaunas Medical University Institute of Cardiology 56
UAB Sorpo 50
Lithuanian Institute of Horticulture 10
UAB Sicor Biotech 76
Institute of Botany 65
Sequencing Income for 2003 - 2010
500000
400000
423284 Lt
122592 €
300000
200000
144270 Lt
41784 €
100000
0
10300 Lt
2983 €
2003-2004
2005-2007
2008-2010
35 th anniversary
54