Mycobacterium Tuberculosis - Diagenode Diagnostics
Mycobacterium Tuberculosis - Diagenode Diagnostics
Mycobacterium Tuberculosis - Diagenode Diagnostics
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PAGE 13<br />
III. Sample Extraction/DNA Isolation<br />
<strong>Diagenode</strong> suggests some appropriate extraction methods:<br />
Nuclisens easyMAG ® System (Biomerieux)<br />
Clinical samples: bronchoalveolar lavage, sputum.<br />
Follow manufacturer’s instructions with the following:<br />
Human sample extracted volume: 200 µl (+ 10 μl of the IC DNA virus culture)<br />
DNA isolation volume: 60 µl<br />
MagNa pure LC system, Roche: MagNa pure LC Total Nucleic Acid Isolation Kit (Ref.:<br />
03038505001)<br />
Clinical samples: bronchoalveolar lavage, sputum.<br />
Follow manufacturer’s instructions with the following:<br />
Human sample extracted volume: 200 µl (+ 10 μl of the IC DNA virus culture)<br />
DNA isolation volume: 60 µl<br />
QIAamp DNA Mini Kit (50) (Blood and Body Fluid Spin Protocol) (Qiagen Ref. 51304)<br />
Clinical samples: bronchoalveolar lavage, sputum.<br />
Follow manufacturer’s instructions with the following:<br />
Human sample extracted volume: 200 µl (+ 10 μl of the IC DNA virus culture)<br />
DNA isolation volume: 60 µl<br />
General extraction information:<br />
If you perform the PCR on the same day, the DNA extraction can be conserved on ice. For a<br />
longer conservation period, DNA must be stored at -20°C.<br />
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