Mycobacterium Tuberculosis - Diagenode Diagnostics
Mycobacterium Tuberculosis - Diagenode Diagnostics
Mycobacterium Tuberculosis - Diagenode Diagnostics
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DIAGENODE MYCOBACTERIUM TUBERCULOSIS REAL-TIME PCR USER MANUAL<br />
CAUTION<br />
<strong>Diagenode</strong> commercializes a qualitative extraction and/or inhibition control. It is the responsible<br />
of the user to optimize the extraction procedure according to the result obtained for this<br />
extraction and/or inhibition control in order to get an appropriate result.<br />
• Virus control extraction must be manipulated using a safety cabinet for a manual extraction<br />
procedure or in a specific area for automated extraction procedures; as suggested by the<br />
manufacturer.<br />
Contamination<br />
• Perform nucleic acid release, isolation and real time amplification in separate laboratory<br />
areas.<br />
• Keep all tubes and vials closed when not in use.<br />
• The PCR laboratory (safety cabinet, DNA/RNA extraction platform, bench coat, etc.) must<br />
be cleaned after each PCR experiment with appropriate solutions.<br />
• The contaminated kits must be thrown in a specific waste bin for biological substances.<br />
• The kit should not be used by somebody displaying symptoms of the disease being detected<br />
by this kit.<br />
• The positive and negative PCR control must be performed with each reaction of PCR.<br />
• Gloves must be worn.<br />
• Aerosol resistant tips must be used for all PCR mixtures.<br />
• Tubes must be harvested before opening.<br />
• Do not pipette any of the materials by mouth. Do not smoke, eat or drink in areas which<br />
specimens or kit reagents are handled.<br />
• The experiment should not be carried out during pregnancy due to risks to the newborn<br />
baby.<br />
CAUTION<br />
Storage of the kit at room temperature can lead to the degradation of the primers / probes /<br />
positive control. This will lead to a decreased sensitivity and we would strongly advise a new kit.<br />
Material Required, Not Provided<br />
• Pipettes<br />
• Sterile pipette tips with filters<br />
• Powder-free gloves<br />
• Vortex mixer<br />
• Desktop centrifuge<br />
• RNase/Dnase-free microcentrifuge tubes (1,5 and/or 2 ml)<br />
• RNase/DNase free water<br />
• DNA isolation kit (see point II. Sample Extraction/DNA Isolation)<br />
• Consumables and accessories (reaction tubes, microcentrifuge, reaction plates,<br />
adhesive seal) for an ABI / Roche / Biorad / Qiagen / Stratagene or Cepheid system<br />
Europe <strong>Diagenode</strong> sa / CHU - Tour GIGA - B34 - 3 e étage // Avenue de l’Hôpital, 1 // 4000 Liège (Sart Tilman) // Belgium // Phone: (+32) 4 364 20 50 // Mail: info@diagenode.com