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Final Prep Answers

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Signal<br />

Chem 155 <strong>Final</strong> review topics and questions:<br />

30. Label the arrows:<br />

Factors that determine performance in chromatography:<br />

a<br />

b<br />

c<br />

d<br />

0 5 10 15 20 25 30<br />

Ret ention T ime / s<br />

Improve resolution a Retention Time c Band width d<br />

Improve efficiency a Determined by K P –<br />

partition coefficient<br />

Improve selectivity b Achieved by change of<br />

mobile phase<br />

Achieved by change of<br />

stationary phase<br />

c<br />

b<br />

b<br />

Set by MP velocity and<br />

SP quality<br />

Set by longitudinal<br />

diffusion<br />

Set by resistance to<br />

mass transfer<br />

Set by variable flow path<br />

d<br />

d<br />

d<br />

d

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