Short Lab Report Examples and Guide

Short Lab Report Guide:The following are excerpted from the lab manual with a coupleof extra figures for your guidance.ABC-UV-VIS Calculations:As indicated above in the theory section, calculate the concentrations of acetone, benzene and chloroformin the 1:100 fold diluted solution.a. using the method of selective eliminationb. using the matrix inversion methodABC-UV-VIS Report:Cover Page: Put your name, partners name, experiment title and results from both analyses a and babove and the unknown letter if available.Attach:Work for the elimination method,Printouts of the Excel work andPrintouts of Cary 50 Bio SpectraABC-FTIR Report:Cover Page: Put the experiment title, your name, partner’s name, the unknown letter and date on a coverpage.Plot the spectra together in a stacked format: Use ‘File’ ‘Load Sample’ and then ‘File’ ‘LoadReference’Page 1 – sample on top, acetone on bottomPage 2 – sample on top, benzene on bottomPage 3 – sample on top, chloroform on bottomOn the page with the spectra:Identify the peaks that both sample and standard have in common.Assign at least two peaks on the standard to a particular vibrational mode.

ABC-FTIR Report: Example of Spectra in Stacked Format with Peaks Labeled.Benzene, neatAcetone, neatChloroform, neatAcetoneBenzeneChloroformCarbonyl C=0 stretch.ABC MixtureAliphatic C-H stretch.

ABC-GCMS Report:Cover page should have your name, your partner’s name, the date and your unknownletter.1. Calculate the concentrations of acetone, benzene and chloroform in themixture using the GC peak integrals, using appropriate internal standard andstandard addition formalism. Show your work. (See next page for details.)2. Attach a chromatogram that shows all of the peaks and their integrals.3. Attach the MS and library match for each peak in ONE of thechromatograms.4. Indicate the likely structures for at least two ions in each mass spectrum.Fluorescence Report:Cover page should have your name, your partner’s name, the date and the experimenttitle.1. Experiment design. Write a sentence or short paragraph indicating theprocedure you devised to produce the 1 μM rhodamine 6G.2. Plots: (see example on next page) Plot both absorbance and fluorescencespectra on the same graph in Excel. Use the following guidelines (8 pts)a. Multiply the fluorescence spectrum values by a constant to give it roughlythe same amplitude as the absorption spectrum.b. Use two different line styles, (i.e. solid and dashed) but no symbols for theplots. Use only black and white.c. No background color.d. No grid lines unless these are necessary to convey information on theplots.e. Appropriate sig-figs on axis labels.f. Use a reasonable font size and a simple font like Arial, Helvetica or Times.g. Give the graph a minimalist appearance. Use the minimum amount of inknecessary to convey the information that you want to for the graph.h. Minimize wasted space in the graph.i. Include a legend indicating which plot is fluorescence and which isemission.3. Indicate the absorption and fluorescence emission processes using arrows of theappropriate relative lengths on a reproduction of the energy level diagram providedbelow.a. Label the axes on the diagram. (2 pts)b. Use arrows on the figure below to indicate:i. Initial state and final states describing the absorbance process. (2 pts)ii. Initial state and final state describing the laser excitation process. (1)iii. Initial state and final states describing the fluorescence emissionprocess. (2 pts)

4. Calculate the photon energy corresponding to the peak in the absorption andemission spectra in the units specified on the report page. (see example on nextpage)a. Answer values correct = +2 ptsb. Proper significant figures and units used = + 2 pts5. In a paragraph or two, explain why the absorption and emission wavelengths aredifferent and why the absorption and emission spectra look similar yet shifted onthe wavelength axis.Fluorescence Experiment: Example Plot.0.10.09λ MAX Abs = 528 nmλ MAX Em = 548 nmAbsorbance0.080.070.060.050.040.030.020.0101.0 µM Rhodamine 6G in water(1.0 cm path)EmissionAbsorbance-0.01400 450 500 550 600 650 700 750 800Wavelength (nm)Fluorescence Experiment : Example Calculations.eV := 1 nm := 10 − 9 ⋅mh := 6.626⋅10 − 34 ⋅J⋅sc 3.00⋅10 8 m:= ⋅sλ 528⋅10 − 9 c:= ⋅mν :=λE:= h⋅νE = 3.765× 10 − 19 J Photon energy in JE⋅ 6.02⋅10 23 − 1⋅mol2.266×10 5 J=molPhoton Energy in J/molE1.602⋅10 − 19 J⋅eV= 2.35eVPhoton Energy in electron volts1λ⋅10 − 7 cm⋅nm= 18939cm − 1Photon Energy in wavenumbers

Jablonski Diagram Page:λ MAX / nmJoulesJoules / molElectron volts (eV)Reciprocal cm (cm -1 )S1AbsorptionEmissionSo