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ASSOCIATION OF HIGH EXPRESSION IN RAT GASTRIC MUCOSAL HEAT SHOCK PROTEIN 70 INDUCED
BY MOXIBUSTION PRETREATMENT WITH PROTECTION AGAINST STRESS INJURY
Xiao-Rong Chang, La Peng, Shou-Xiang Yi, Yan Peng, Jie Yan
University of Traditional Chinese Medicine
Aim: To study the effect of moxibustion on Zusanli or Liangmeng point on gastric mucosa injury in stressinduced
ulcer rats and its correlation with the expression of heat shock protein 70 (HSP70).
Methods: Sixty healthy SD rats (30 males, 30 females) were divided into control group, injury model group, Zushanli
point group, Liangmeng point group. Stress gastric ulcer model was induced by binding cold stress method. Gastric
mucosa ulcer injury (UI) index was calculated by Guth method. Gastric mucosa blood flow (GMBF) was recorded with
a biological signal analyzer. Protein content and gene expression in gastric mucosal HSP70 were detected by
immunohistochemistry (IHC) and reverse transcription polymerase chain reaction (RT-PCR). Thiobarbital method
was used to determine malondialdehyde (MDA) content. Gastric mucosal endothelin (ET) and prostaglandin E2
(PGE2) were analyzed by radioimmunoassay.
Results: High gastric mucosal UI index, high HSP70 expression, low GMBF and PGF2, elevated MDA and ET
were observed in gastric mucosa of rats subjected to cold stress. Moxibustion on Zusanli or Liangmeng point
decreased rat gastric mucosal UI index, MDA and ET. Conversely, the expression of HSP70, GMBF, and PGE2 was
elevated in gastric mucosa after pretreatment with moxibustion on Zusanli or Liangmeng point. The observed
parameters were significantly different between Zusanli and Liangmeng points.
Conclusion: Pretreatment with moxibustion onZusanli or Liangmeng point protects gastric mucosa against stress
injury. This protection is associated with the higher expression of HSP70 mRNA and protein, leading to release of
PGE2 and inhibition of MDA and ET, impairment of gastric mucosal index. Key words: Moxibustion Zusanli
Liangmeng Stress
RESIDENCY PROGRAM ON ACUPUNCTURE – A REALITY IN BRAZIL
Jéssica Maria Costi, Li Shih Min, Ricardo Horta Gonçalves, Graciela de Oliveira, Richter Schmidt, Eduardo Marten
Teixeira, Ari Ojeda More
Hospital Regional de São José
Introduction: Residency programs on acupuncture have been functioning in Brazil since 2004. The present report
brings the experience of the third program, started in 2005, at Hospital Regional de Sao Jose, Brazil.
Background: The 2-year program includes a comprehensive list of traineeships in basic areas (internal medicine,
neurology, orthopedics, gynecology-obstetrics) to be carried out in the first 6 months. From then, the specific practice
in acupuncture begins, performed in several outpatient departments, with different staff collaborators. Along with the
acupuncture practice, the traineeship in physical medicine and rehabilitation is accomplished. Also as part of the
program, it is expected from the resident to attend classes about traditional Chinese medicine, as well as reading and
discussion about scientific literature, including original papers and revision articles. By the end of the 2nd year, the
resident presents an original scientific work to be performed as a conclusion of the program.
Discussion: This is an extensive program, to be carried out in a general hospital because of the clinical and
surgical basic areas, with many professionals involved. Considering the amount of demands, the resident can be very
required during the 2-year program.
Conclusion: The Brazilian residency program on acupuncture is comprehensive, extensive and detailed. Besides
the skills in diagnosis and practice of acupuncture, the residents are also trained in basic areas. Thus the specialists
formed by such program are expected to have an optimum knowledge of both western and eastern medicine,
enhancing and contributing to the improvement in formation and competence of medical acupuncturists.
A PHARMACOLOGICAL STUDY OF SKIN ELECTRIC CURRENT AS BASE OF THE RYODORAKU MERIDIAN
(EMI) MEASUREMENT -- EFFECT AFTER SYMPATHICOLYTIC DRUG ADMINISTRATION FOR
EXPERIMENTAL ANIMAL
Hiroshi Endo 1,2 , Wang Cai Yuan 1 , Takayuki Nakayoshi 1 , Noboru Kibi 1 , Hideaki Higashino 2
1
Kansai University of Health Sciences (Acupuncture discipline), 2 Kinki University, School of Medicine, Department of
Pharmacology
Aim of Investigation: M.D. and L. Acu. of Ryodoraku medical science measures fluctuation of skin electric current
by autonomic imbalance and applies that to diagnosis of Traditional Chinese Medicine (meridian). However, it is
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