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Mycoplasma pneumoniae Chlamydophila ... - Diagenode Diagnostics

Mycoplasma pneumoniae Chlamydophila ... - Diagenode Diagnostics

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VI . Interpretation of results<br />

Interpretation of results<br />

DNA extraction & PCR<br />

inhibition control<br />

<strong>Chlamydophila</strong><br />

<strong>pneumoniae</strong><br />

<strong>Mycoplasma</strong> <strong>pneumoniae</strong><br />

Signal detection<br />

Signal detection<br />

Signal detection<br />

FAM<br />

Orange dye<br />

Yellow dye<br />

Cy5<br />

The sample is positive:<br />

<strong>Mycoplasma</strong> <strong>pneumoniae</strong><br />

and negative:<br />

<strong>Chlamydophila</strong> <strong>pneumoniae</strong><br />

Signal is above or below the<br />

threshold<br />

Signal is above the threshold Signal is below the threshold<br />

The sample is negative:<br />

<strong>Mycoplasma</strong> <strong>pneumoniae</strong><br />

and positive:<br />

<strong>Chlamydophila</strong> <strong>pneumoniae</strong><br />

Signal is above or below the<br />

threshold<br />

Signal is below the threshold Signal is above the threshold<br />

The sample is positive:<br />

<strong>Mycoplasma</strong> <strong>pneumoniae</strong><br />

<strong>Chlamydophila</strong> <strong>pneumoniae</strong><br />

Signal is above or below the<br />

threshold<br />

Signal is above the threshold Signal is above the threshold<br />

The sample is negative:<br />

<strong>Mycoplasma</strong> <strong>pneumoniae</strong><br />

<strong>Chlamydophila</strong> <strong>pneumoniae</strong><br />

Signal is below the threshold Signal is below the threshold Signal is above the threshold<br />

The sample is invalid .<br />

See Troubleshooting guide<br />

Signal is below the threshold Signal is below the threshold Signal is below the threshold<br />

PAGE 19<br />

www.diagenodediagnostics.com

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