Accelerate your research: NEB quick cloning Box Hf! - Lab-JOT

Quick cloning
Accelerate your research:
NEB Quick Cloning Box HF!
To speed up your cloning experiments, choose the “NEB Quick Cloning Box-HF”
- a selection of the most important cloning enzymes and tools in optimized “Quick”
formulations at a price you can’t afford to miss! The “NEB Quick Cloning Box-HF”
will allow you to achieve results faster without compromising on the quality or consistency
of your data. Cloning in just one day becomes a reality!
Due to the value pricing the NEB Quick Cloning Box-HF is also the ideal start for
new laboratories, student practical courses, schools etc.
DNA Cloning
DNA Amplification & PCR
Epigenetics
RNA Analysis
Sample Prep for Next Gen Sequencing
Protein Expression & Analysis
Cellular Analysis
Advantages:
+
• Selection & Value Pricing
- the most commonly used cloning
enzymes and tools at a price you
can’t afford to miss
• HF Restriction Enzymes
E engineered for performance
R dramatically reduced star activity
C Time-Saver qualified
4 optimized for compatibility
• Quick Kits
- optimized for faster results without
compromises on quality
The NEB Quick-Cloning Box HF contains:
NEB# ProduCt units
R3136 S BamHI-HF r E R C 10.000 u
R3101 S EcoRI-HF r E R C 10.000 u
R3104 S HindIII-HF r E R C 10.000 u
R3189 S NotI-HF r E R C 500 u
M0531 S Phusion ® High-Fidelity PCR Master Mix with HF Buffer r 100 rxn (50 µl vol)
M0289 S Antarctic Phosphatase r 1.000 u
E1201 S Quick Blunting Kit r 20 rxn
M2200 S Quick Ligation Kit r 30 rxn
N0469 S Quick-Load ® 2-Log DNA Ladder 125-250 lanes
T0400 S NEBcool Lab-Bench Cooler 1 cooler
NEB Lab-Timer
1 timer
incl. all NEBuffers, BSA, Gel-Loadingbuffer etc.
• Phusion ® High-Fidelity
DNA Polymerase
- fast and accurate PCRs
• Complete Tool Box
- contains DNA Ladder, NEBuffers,
BSA, Gel Loading Buffer etc.
B
Cloned at NEBiolabs
r
Recombinant Enzyme
C
Time-Saver Qualified
4
Optimum Buffer
b
Requires BSA
New icons:
E
Indicates that the
enzyme has been
engineered
6
Incubation Temperature
a
Methylation Sensitivity
y
Heat Inactivation
p
Blue/White Certified
R
Indicates that the
enzyme has reduced
star activity
E1114 S NEB Quick-Cloning Box HF only 399,- € *
*special price - no further discounts apply!

Quick Cloning
The “NEB Quick Cloning Box-HF” contains the reagents for your complete cloning needs:
STEPS
PCR amplification
of DNA fragment
of interest
Restriction
Digest in
NEBuffer 4
(5 min)
Quick
Blunting
(15 min)
Dephosphorylation
(vector only)
(30 min)
Quick
Ligation
(5 min)
Transformation &
outgrowth in
fast growing E. coli
( digest substrate DNA in only 5 min
Advantages
• Engineered for Performance E
• High Fidelity (HF) Enyzmes with dramatically
reduced star activity R
• Time-Saver qualified for 5 min digests C
• Optimized for highest buffer compatibility
(NEBuffer 4 4 )
• 100% compatible with the
NEB Restriction Enzyme Buffer System!
A Small Selection of >160 Time-Saver
Qualified Restriction Enzymes from NEB
Digest
Enzyme in
5 min
BamHI-HF C 3
BglII C 3
EcoRI-HF C 3
HindIII-HF C 3
NcoI-HF C 3
NotI-HF C 3
PstI-HF C 3
Digest
Enzyme in
5 min
SacI-HF C 3
SalI-HF C 3
SmaI C 3
SpeI C 3
SphI-HF C 3
XbaI C 3
XhoI C 3
Antarctic Phosphatase
> dephosphorylation of DNA, RNA, dNTPs
and proteins
Advantages
• Active in all NEBuffers when AnP buffer
is added - no purification of DNA prior or
after use needed
• Recombinant enzyme active on DNA, RNA,
protein, dNTPs and pyrophosphate
• 100% heat inactivated in only 5 min
Phusion ® High-Fidelity
DNA Polymerase
> fast, accurate and robust PCR
Advantages:
• Robust Reactions – Maximal success
with minimal optimization
• Extreme Fidelity – Highest of any
available thermostable polymerase
(50X greater than Taq)
• High Speed – Dramatically reduced extension
times (10X faster than Pfu)
Quick Ligation Kit
> ligation in 5 minutes at room temperature
Advantages
• 5 minute reactions for cohesive or blunt
ends
• Convenience of ligations performed at
room temperature
• Suitable for all common ligation reactions
Quick Blunting Kit
> blunting DNA fragments in up to 15 min
Advantages
• 15 min reactions for blunting RE digests;
30 min for PCR products or sheared/
nebulized DNA
• Reactions are performed at room
temperature in a ready-to-use mix
Time-Saver enzymes (icon C) will digest 1 µg of the specified
DNA in 5 minutes with 1 µl of enzyme under recommended
reaction conditions. All NEB High Fidelity (HF ) Restriction
Enzymes are also Time-Saver qualified.
This product is manufactured by New England Biolabs, Inc. under agreement
with, and under the performance specifications of Thermo Fisher Scientific.
Phusion DNA Polymerase was developed by Finnzymes Oy, now a part of
Thermo Fisher Scientific. Phusion ® is a registered trademark and property of
Thermo Fisher Scientific.
www.neb.com
New England Biolabs (UK) Ltd.
www.neb.uk.com
New England Biolabs France
www.neb-online.fr
For a complete list of international offices and distributors, please visit www.neb.com
New England Biolabs GmbH, Germany
www.neb-online.de