eXceptional Performance - Lab-JOT

XP®Monoclonal Antibodiesexclusively from Cell Signaling Technologywww.cellsignal.comUNPARALLELED PRODUCT QUALITY, VALIDATION, AND TECHNICAL SUPPORT

XP®MonoclonalAntibodieseXceptional Performance XP ® monoclonal antibodies are a line of highquality rabbit monoclonal antibodies exclusivelyavailable from Cell Signaling Technology(CST). Any product labeled with XP has beencarefully selected based on superior performancein the most relevant applications.XP monoclonal antibodies are generated usingXMT ® technology, a proprietary monoclonaltechnology developed at CST. This technologyprovides access to a broad range ofantibody-producing B cells unattainable withtraditional monoclonal technologies, allowingmore comprehensive screening and the identificationof XP monoclonal antibodies with:Phospho-Akt (Ser473) (D9E)XP ® Rabbit mAb #4060 is anexample of an antibody withsuperior performance in a widerange of tested applications.EventsAPhospho-Akt (Ser473)CkDa100806050806050BPC-3– +– +– –NIH/3T3– –– –– +Phospho-Akt(Ser473)AktwortmanninLY294002PDGFeXceptional specificityAs with all CST antibodies, the antibody isspecific to your target of interest, saving youvaluable time and resources.+ eXceptional sensitivityThe antibody will provide a stronger signal for yourtarget protein in cells and tissues, allowing you tomonitor expression of low levels of endogenousproteins, saving you valuable materials.DE+ eXceptional stability and reproducibilityXMT technology combined with our stringentquality control ensures maximum lot-to-lotconsistency and the most reproducible results.= eXceptional PerformanceXMT technology coupled with our extensiveantibody validation and stringent qualitycontrol delivers XP monoclonal antibodies witheXceptional Performance in the widest range ofapplications.Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060: Flowcytometric analysis of Jurkat cells (A), untreated (green) or treatedwith LY294002 #9901, wortmannin #9951, and U0126 #9903 (blue),using #4060 compared to a nonspecific negative control antibody (red).Western blot analysis (B) of extracts from PC-3 cells, untreated or treatedwith LY294002 #9901/wortmannin #9951, and NIH/3T3 cells, serumstarvedor PDGF-treated, using #4060 (upper) or Akt (pan) (C67E7)Rabbit mAb #4691 (lower). IHC analysis of paraffin-embedded PTENheterozygous mutant mouse endometrium (C) using #4060 . (Tissuesection courtesy of Dr. Sabina Signoretti, Brigham and Women’s Hospital,Harvard Medical School, Boston, MA.) Confocal IF analysis of C2C12cells, treated with LY294002 #9901 (D) or insulin (E), using #4060(green). Actin filaments were labeled with Alexa Fluor ® 555 phalloidin(red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).Visit our website for more experimental details, additional information,and the most up-to-date list of available XP ® Monoclonal Antibodies.

XP ® Monoclonal AntibodySuggested toApplications Reactivity Replace Antibodies#5053 Argonaute 1 (D84G10) XP ® Rabbit mAb W, IP, IHC-P H, M, R, Mk #9388#5318 AIF (D39D2) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (B, Dg) #4642New #5203 AKAP1 (D9C5) XP ® Rabbit mAb W, IP, IF-IC H, M, R#4060 Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb W, IP, IHC-P, IHC-F,IF-IC, F#4071 Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb F(Alexa Fluor ® 488 Conjugate)#4075 Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb F(Alexa Fluor ® 647 Conjugate)#5012 Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb(Biotinylated)#5171 Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb(HRP Conjugate)#5315 Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb(PE Conjugate)#4070 Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb(Sepharose Bead Conjugate)H, M, R, Hm, Mk, Dm,Z, B, (C, X, Dg, Pg)H, M, R, Hm, Mk, Dm,Z, B, (C, X, Dg, Pg)H, M, R, Hm, Mk, Dm,Z, B, (C, X, Dg, Pg)W, F H, M, R, Hm, Mk, Dm,Z, B, (C, X, Dg, Pg)WH, M, R, Hm, Mk, Dm,Z, B, (C, X, Dg, Pg)FH, M, R, Hm, Mk, Dm,Z, B, (C, X, Dg, Pg)IPH, M, R, Hm, Mk, Dm,Z, B, (C, X, Dg, Pg)#3787, #4051,#4058, #9271#4336 AML1 (D33G6) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H #4334New #8070 AMPA Receptor (GluR 4) (D41A11) XP ® Rabbit mAb W, IP, IF-F H, M, R#3796 α-Amylase (D55H10) XP ® Rabbit mAb W, IP, IHC-P H, R, (M) #4017#5153 Androgen Receptor (D6F11) XP ® Rabbit mAb W, IHC-P, IF-IC, F H#3858 APPL1 (D83H4) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #3276#5019 ASH2L (D93F6) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (Dm)#2914 Phospho-Aurora A (Thr288)/Aurora BW, IF-IC, F H, M, R(Thr232)/Aurora C (Thr198) (D13A11)XP ® Rabbit mAbNew #2995 Phospho-Aurora A (Thr288)/Aurora BWH, M, R(Thr232)/Aurora C (Thr198) (D13A11)XP ® Rabbit mAb (Biotinylated)#5601 βIG-H3 (D31B8) XP ® Rabbit mAb W, IP, IF-IC H #2719New #6964 Bmi1 (D20B7) XP ® Rabbit mAb W, IP, IF-IC, ChIP H, Mk #2830New #8975 C11orf59 (D11H6) XP ® Rabbit mAb W, IP, IHC-P, IF-IC H, M, R, MkNew #5864 CA12 (D75C6) XP ® Rabbit mAb W, IP, F H #5865#2500 VE-Cadherin (D87F2) XP ® Rabbit mAb W, IP, IF-IC, F H, Dm, B, Pg, (Mk) #2158#4176 Phospho-β-Catenin (Ser675)W, IP, IF-F, IF-IC H, (M, R, C, X, Z) #9567(D2F1) XP ® Rabbit mAb#3267 Caveolin-1 (D46G3) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, R, Hm, Mk, #3238B, Dg#4793 CDC37 (D11A3) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #3604, #3618,#4222#5480 CHAF1A (D77D5) XP ® Rabbit mAb W, IP, IF-IC H, Mk#4796 Clathrin Heavy Chain (D3C6) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #2410#5664 CNPase (D83E10) XP ® Rabbit mAb W, IP, IF-F H, M, RNew #5714 CNPase (D83E10) XP ® Rabbit mAbIF-FH, M, R(Alexa Fluor ® 488 Conjugate)New #5715 CNPase (D83E10) XP ® Rabbit mAbIF-FH, M, R(Alexa Fluor ® 555 Conjugate)New #5716 CNPase (D83E10) XP ® Rabbit mAbIF-FH, M, R(Alexa Fluor ® 647 Conjugate)#5175 Cofilin (D3F9) XP ® Rabbit mAb W, IF-IC H, M, R, Mk, Dg #3312, #3318#2336#2337

XP ® Monoclonal AntibodySuggested toApplications Reactivity Replace Antibodies#3417 CTCF (D1A7) XP ® Rabbit mAb W, IP, IF-IC, ChIP H, R, Mk, (B)#3418 CTCF (D31H2) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, H, M, R, Mk, (B) #2899ChIP#3300 Phospho-Cyclin D1 (Thr286)W, IP, IF-IC, F H, (Mk) #2921(D29B3) XP ® Rabbit mAb#5583 DDR1 (D1G6) XP ® Rabbit mAb W, IP, IHC-P, IF-IC H, M, R, Mk #3917#5332 Desmin (D93F5) XP ® Rabbit mAb W, IF-F, IF-IC H, M, R, (Mk)#5032 DNMT1 (D63A6) XP ® Rabbit mAb W, IF-IC H, M, R, Mk, (B)New #5684 EAAT1 (D44E2) XP ® Rabbit mAb W, IP, IF-F H, M, R #4166EAAT1 (D44E2) XP ® Rabbit mAb #5684:Confocal IF analysis of rat brain using #5684(green). Red = Propidium Iodide (fluorescentDNA dye).Glucagon (D16G10) XP ® Rabbit mAb ▶#8233: Confocal IF analysis of mousepancreas using #8233 (green). Actinfilaments were labeled with DY-554phalloidin (red). Blue pseudocolor =DRAQ5 ® #4084 (fluorescent DNA dye).#3777 Phospho-EGF Receptor (Tyr1068)(D7A5) XP ® Rabbit mAbNew #8543 Phospho-EGF Receptor (Tyr1068)(D7A5) XP ® Rabbit mAb (Biotinylated)#2085 EGF Receptor (E746-A750del Specific)(6B6) XP ® Rabbit mAbW, IHC-P, IF-IC, F H, M, R, Mk #2234, #2236WW, IP, IHC-P, IF-IC,IF-P, FH, M, R, Mk#4267 EGF Receptor (D38B1) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, Mk #2232, #2239,#2256, #2646,#4405#5616 EGF Receptor (D38B1) XP ® Rabbit mAbIF-IC, FH, M, Mk(Alexa Fluor ® 488 Conjugate)#5108 EGF Receptor (D38B1) XP ® Rabbit mAbIF-ICH, M, Mk(Alexa Fluor ® 555 Conjugate)#5588 EGF Receptor (D38B1) XP ® Rabbit mAbIF-IC, FH, M, Mk(Alexa Fluor ® 647 Conjugate)#6627 EGF Receptor (D38B1) XP ® Rabbit mAbWH, M, Mk(Biotinylated)New #6937 EGF Receptor (D38B1) XP ® Rabbit mAbIPH, M, Mk(Magnetic Bead Conjugate)New #8839 EGF Receptor (D38B1) XP ® Rabbit mAbFH, M, Mk(PE Conjugate)#5735 EGF Receptor (D38B1) XP ® Rabbit mAbIPH, M, Mk(Sepharose Bead Conjugate)#3398 Phospho-eIF2α (Ser51)W, IP, IHC-P H, M, R, Mk, Dm #9721(D9G8) XP ® Rabbit mAbNew #5199 Phospho-eIF2α (Ser51)WH, M, R, Mk, Dm(D9G8) XP ® Rabbit mAb (Biotinylated)#5324 eIF2α (D7D3) XP ® Rabbit mAb W, IP, IHC-P, IF-P H, M, R, Mk #2103, #9722#3411 eIF3A (D51F4) XP ® Rabbit mAb W, IP, IHC-P, IF-IC H, M, R, Mk #2538#3413 eIF3H (D9C1) XP ® Rabbit mAb W, IP, IHC-P, IF-IC H, M, R, Mk#5169 eIF4G2/p97 (D88B6) XP ® Rabbit mAb W, IP, IF-IC, F H, M, R, MkH4Applications Key: W Western / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F FlowCytometry / ChIP Chromatin Immunoprecipitation / (-IC Immunocytochemistry, -P Paraffin, -F Frozen) / E-P Peptide ELISA

XP ® Monoclonal AntibodySuggested toApplications Reactivity Replace Antibodies#3469 eIF4H (D85F2) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #2444#3833 eIF6 (D16E9) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #3263New #6997 EphA2 (D4A2) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #3974#3798 ERp44 (D17A6) XP ® Rabbit mAb W, IHC-P H, M, R, Mk #2886#5033 ERp72 (D70D12) XP ® Rabbit mAb W, IF-IC, F H, M, R, Mk#5246 Ezh2 (D2C9) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, H, M, R, Mk #3147, #4905ChIP#3544 FABP4 (D25B3) XP ® Rabbit mAb W, IP, IF-IC M, (H) #2120#5348 Phospho-c-Fos (Ser32)(D82C12) XP ® Rabbit mAbW, IP, IF-IC, F H, M, R, (Hm, Mk,B, Pg)#5436 FoxM1 (D12D5) XP ® Rabbit mAb W, IF-IC H #3948#4402 FoxP1 (D35D10) XP ® Rabbit mAb W, IP, IHC-P, F H, M, R, MkNew #5843 GAD2 (D5G2) XP ® Rabbit mAb W, IP, IF-F H, M, R#5174 GAPDH (D16H11) XP ® Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk #2118#4589 GATA-1 (D24E4) XP ® Rabbit mAb W, IP, IF-IC, F H #4591#3535 GATA-1 (D52H6) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, R #4591#5852 GATA-3 (D13C9) XP ® Rabbit mAb W, IF-IC, F H, (Mk)#5851 GATA-6 (D61E4) XP ® Rabbit mAb W, IF-IC H #4253#2956 GFP (D5.1) XP ® Rabbit mAb W, IHC-P, IF-IC, F All #2555#2912 GFP (D5.1) XP ® Rabbit mAb (Biotinylated) W All#2037 GFP (D5.1) XP ® Rabbit mAb (HRP Conjugate) W AllNew #8233 Glucagon (D16G10) XP ® Rabbit mAb IHC-P, IHC-F, IF-F H, M, R#3593 Grp75 (D13H4) XP ® Rabbit mAb W, IP, IHC-P, IF-IC H, M, Mk #2816#4818 GSK-3α (D80D1) XP ® Rabbit mAb IF-IC, F H, M, (R) #9338#5676 GSK-3α/β (D75D3) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Hm, Mk#5558 Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb W, IP, IF-IC, F H, M, R, Hm #9322, #9336#4833 HAUSP (D17C6) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #3277#4290 HER2/ErbB2 (D8F12) XP ® Rabbit mAb W, IHC-P H, (M, R)New #8609 HER2/ErbB2 (D8F12) XP ® Rabbit mAb (Biotinylated) W H, (M, R)#3434 Hydroxy-HIF-1α (Pro564)(D43B5) XP ® Rabbit mAbW, IP, IF-IC H, (M, R, Mk, C, X,Z, Pg)#5537 HIF-1β/ARNT (D28F3) XP ® Rabbit mAb W, IP, IHC-P H, M, R, MkNew #7631 Histone H2A.X (D17A3) XP ® Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk #2595New #8228 Phospho-Histone H2A.X (Ser139) (20E3) Rabbit IF-ICH, M, R, MkmAb (Alexa Fluor ® 555 Conjugate)#5546 Ubiquityl-Histone H2B (Lys120) (D11)W, ChIP H, M, R, MkXP ® Rabbit mAb#3377 Phospho-Histone H3 (Ser10) (D2C8)W, IF-IC, F H, M, R, Mk, Z #9701XP ® Rabbit mAb#3465 Phospho-Histone H3 (Ser10) (D2C8)IF-IC, F H, M, R, Mk, Z #9708XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate)#3475 Phospho-Histone H3 (Ser10) (D2C8)IF-ICH, M, R, Mk, ZXP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate)#3458 Phospho-Histone H3 (Ser10) (D2C8)IF-IC, F H, M, R, Mk, Z #9716XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate)#3642 Phospho-Histone H3 (Ser10)W, IF-F, IF-IC, F H, M, R, Mk, Z(D2C8) XP ® Rabbit mAb (Biotinylated)#5764 Phospho-Histone H3 (Ser10)(D2C8) XP ® Rabbit mAb (PE Conjugate)FH, M, R, Mk, ZReactivity Key: H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish /B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology5

XP ® Monoclonal Antibody Applications Reactivity#5326 Mono-Methyl-Histone H3 (Lys4)W, IF-IC, ChIP H, M, R, Mk(D1A9) XP ® Rabbit mAb#4658 Di-Methyl-Histone H3 (Lys9)(D85B4) XP ® Rabbit mAb#4473 Pan-Methyl-Histone H3 (Lys9)(D54) XP ® Rabbit mAbNew #6951 Pan-Methyl-Histone H3 (Lys9)(D54) XP ® Rabbit mAb (HRP Conjugate)#9728 Di-Methyl-Histone H3 (Lys27)(D18C8) XP ® Rabbit mAb#4909 Tri-Methyl-Histone H3 (Lys36)(D5A7) XP ® Rabbit mAb#5427 Di-Methyl-Histone H3 (Lys79)(D15E8) XP ® Rabbit mAbW, IP, IF-IC, ChIP H, M, R, Mk, (Dm, X,Z, B, Pg, Sc)#9753W, IP, IF-IC, ChIP H, M, R, Mk, (C, Dm, #4069X, Z, B, Pg, Sc)WH, M, R, Mk, (C, Dm,X, Z, B, Pg, Sc)W, IF-IC, ChIP H, M, R, Mk #9755W, ChIP H, M, R, Mk, (Hm, C,Dm, X, Z, B)W, ChIP H, M, R, Mk#4499 Histone H3 (D1H2) XP ® Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk, (Hm, C,Dm, X, Z, B)New #5748 Histone H3 (D1H2) XP ® Rabbit mAb (Biotinylated) W H, M, R, Mk, (Hm, C,Dm, X, Z, B)#4620 Histone H3 (D2B12) XP ® Rabbit mAb(ChIP Formulated)ChIP H, M, (R, Hm, Mk, C,Dm, X, Z, B)Suggested toReplace Antibodies#3638, #3680,#9715, #9717New #5545 hnRNP A0 (D8A3) XP ® Rabbit mAb W, IP, IF-IC, F H, M, R, Mk #4046New #5670 HOP (D10E2) XP ® Rabbit mAb W, IF-IC, F H, Mk, B #4464, #5669#3892 HS1 (D5A9) XP ® Rabbit mAb (Rodent Specific) W, IP, IHC-P, F M, R #4557#3890 HS1 (D83A8) XP ® Rabbit mAb (Human Specific) W, IP, IHC-P, IF-IC, F H #4503New #5656 Huntingtin (D7F7) XP ® Rabbit mAb W, IP, IF-F H, M, R #2773#3416 IKKε (D61F9) XP ® Rabbit mAb W, IP, IF-IC, F M, R #2690#4504 IRAK1 (D51G7) XP ® Rabbit mAb W, IP, IF-IC H, M, Mk #4359, #4362New #6918 IRAP (D7C5) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #3808#3230 Jak2 (D2E12) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, R, (Mk)#4040 Jak2 (D2E12) XP ® Rabbit mAb (Biotinylated) W, F H, M, R, (Mk)#4089 Jak2 (D2E12) XP ® Rabbit mAbIPH, M, R, (Mk)(Sepharose Bead Conjugate)#3876 JARID1A (D28B10) XP ® Rabbit mAb W, IP, IF-IC H, M, (R, B)New #5519 JIP4/SPAG9 (D72F4) XP ® Rabbit mAb W, IP, IF-IC, F H, M, R, Mk#3270 Phospho-c-Jun (Ser73) (D47G9) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, R, Mk #9164#3074 c-Kit (D13A2) XP ® Rabbit mAb W, IP, IF-IC H, M #3308, #3392New #5749 c-Kit (D13A2) XP ® Rabbit mAb (Biotinylated) W H, M#4543 Keratin 17 (D73C7) XP ® Rabbit mAb W, IF-IC, F H, M, R, Mk, (Dg)#3984 Keratin 17/19 (D32D9) XP ® Rabbit mAb W, IHC-P H, M, R, (Mk, B, Pg)#4599 LC3A (D50G8) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, R, (Mk, Dg)#3868 LC3B (D11) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, R, (Mk, B, Pg) #2775#2984 Lck (D88) XP ® Rabbit mAb W, IHC-P H #2714, #2752,#2787#3695 LIN28A (D84C11) XP ® Rabbit mAb W, IF-IC, F H, (R, Mk)#5471 Livin (D61D1) XP ® Rabbit mAb W, IP, IF-IC H#4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)(D13.14.4E) XP ® Rabbit mAb#4344 Phospho-p44/42 MAPK (Erk1/2)(Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb(Alexa Fluor ® 488 Conjugate)W, IP, IHC-P, IF-IC, F H, M, R, Hm, Mk, Mi,Dm, Z, B, Dg, Pg, ScFH, M, R, Hm, Mk, Mi,Dm, Z, B, Dg, Pg, Sc#2650#4376, #4377,#9101, #9106#43746Applications Key: W Western / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F FlowCytometry / ChIP Chromatin Immunoprecipitation / (-IC Immunocytochemistry, -P Paraffin, -F Frozen) / E-P Peptide ELISA

XP ® Monoclonal Antibody Applications Reactivity#4284 Phospho-p44/42 MAPK (Erk1/2)F(Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb(Alexa Fluor ® 647 Conjugate)#4094 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)(D13.14.4E) XP ® Rabbit mAb (Biotinylated)#5682 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)(D13.14.4E) XP ® Rabbit mAb (PE Conjugate)#3510 Phospho-p44/42 MAPK (Erk1/2)(Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb(Sepharose Bead Conjugate)#4511 Phospho-p38 MAPK (Thr180/Tyr182)(D3F9) XP ® Rabbit mAbH, M, R, Hm, Mk, Mi,Dm, Z, B, Dg, Pg, ScW, F H, M, R, Hm, Mk, Mi,Dm, Z, B, Dg, Pg, ScFH, M, R, Hm, Mk, Mi,Dm, Z, B, Dg, Pg, ScIPH, M, R, Hm, Mk, Mi,Dm, Z, B, Dg, Pg, ScW, IP, IHC-P, IF-IC, F H, M, R, Mk, Sc, (Hm,C, Z, B, Pg)Suggested toReplace Antibodies#4375#9109#4631, #9211,#9215, #9216#5607 MARCKS (D88D11) XP ® Rabbit mAb W, IHC-P, IF-IC, F H, Mk#3619 MCM2 (D7G11) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, H, M, R, Mk #4007ChIP#3735 MCM7 (D10A11) XP ® Rabbit mAb W, IP, IHC-P, IF-IC H, M, R, Hm, Mk, Dg #4018#3456 MeCP2 (D4F3) XP ® Rabbit mAb W, IP, IHC-P, IF-IC H, M, R, Mk#5030 MEF2C (D80C1) XP ® Rabbit mAb W, IP, IF-IC H, MNew #6891 Menin (D45B1) XP ® Rabbit mAb W, IF-IC H, M, R, Mk, (B, Pg)#4319 Mer (D21F11) XP ® Rabbit mAb W, IP, IF-IC H #9178#3077 Phospho-Met (Tyr1234/1235) (D26) XP ®W, IP, IHC-P, IHC-F H, M, R #3126, #3129Rabbit mAb#4033 Phospho-Met (Tyr1234/1235) (D26) XP ®WH, M, RRabbit mAb (Biotinylated)#4091 Phospho-Met (Tyr1234/1235) (D26) XP ®IPH, M, RRabbit mAb (Sepharose Bead Conjugate)New #8198 Met (D1C2) XP ® Rabbit mAbW, IHC-P, IHC-F, HIF-IC, FNew #8041 Met (D1C2) XP ® Rabbit mAb (Biotinylated) W HNew #5940 Mili (D14F5) XP ® Rabbit mAb W, IP, IHC-P, IF-F M #2071New #6915 Miwi (D92B7) XP ® Rabbit mAb W, IP, IF-F M, (H, R)#3801 MMP-7 (D4H5) XP ® Rabbit mAb W, IHC-P M, R#2066 MRPL11 (D68F2) XP ® Rabbit mAb W, IP, IHC-P, IF-IC H, M, R, Mk #2199#2017 MSH2 (D24B5) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, Mk#5424 MSH6 (D60G2) XP ® Rabbit mAb W, IF-IC H, Mk #3995, #3996#3679 MSK2 (D41A4) XP ® Rabbit mAb W, IP, IF-IC H#5647 MTA1 (D40D1) XP ® Rabbit mAb W, IHC-P H, M, R, MkPhospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ®Rabbit mAb #4370: IHC analysis of paraffin-embedded human lungcarcinoma, untreated (left) or λ phosphatase-treated (right), using #4370.Mili (D14F5) XP ® Rabbit mAb #5940: ConfocalIF analysis of mouse testis using #5940 (green) andPan-Keratin (C11) Mouse mAb #4545 (red). Bluepseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).Reactivity Key: H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish /B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology7

XP ® Monoclonal AntibodySuggested toApplications Reactivity Replace Antibodies#5536 Phospho-mTOR (Ser2448) (D9C2) XP ® Rabbit mAb W, IP, IF-IC H, M, Mk, (R) #2971#5663 Musashi-1 (D46A8) XP ® Rabbit mAb W, IF-F H, M, R #2154#5605 c-Myc (D84C12) XP ® Rabbit mAb W, IP, IF-IC, F H, M, R, (Mk, Dg, Pg) #9402#4903 Nanog (D73G4) XP ® Rabbit mAb W, IHC-P, IF-IC, F H, (Mk) #4893#5448 Nanog (D73G4) XP ® Rabbit mAbIF-IC, FH, (Mk)(Alexa Fluor ® 647 Conjugate)#5232 Nanog (D73G4) XP ® Rabbit mAb (ChIP Formulated) ChIP HNew #8237 NCS1 (D12D2) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Hm, Mk#5482 Phospho-NDRG1 (Thr346) (D98G11)W, IHC-P, IF-IC, F H, M, R, Mk #3217XP ® Rabbit mAbNew #6992 Phospho-NDRG1 (Thr346) (D98G11) XP ®IF-IC, FH, M, R, MkRabbit mAb (Alexa Fluor ® 488 Conjugate)New #9851 Phospho-NDRG1 (Thr346) (D98G11) XP ®IF-ICH, M, R, MkRabbit mAb (Alexa Fluor ® 555 Conjugate)New #7497 Phospho-NDRG1 (Thr346) (D98G11) XP ®IF-IC, FH, M, R, MkRabbit mAb (Alexa Fluor ® 647 Conjugate)New #5861 NFAT1 (D43B1) XP ® Rabbit mAb W, IP, IF-IC, F H, MNew #8242 NF-κB p65 (D14E12) XP ® Rabbit mAbW, IP, IHC-P, IF-IC, H, M, R, Hm, Mk, DgF, ChIP#3608 Notch1 (D1E11) XP ® Rabbit mAb W, IHC-P H, M, R #3268, #3439,#3447#4380 Notch1 (D6F11) XP ® Rabbit mAb W, IF-IC, F H, M, R#4530 Notch2 (D67C8) XP ® Rabbit mAb W, IP, IF-IC H, R #2420#5732 Notch2 (D76A6) XP ® Rabbit mAb W, IP, IF-IC, F H, M, R #2420#3366 Neuropilin-2 (D39A5) XP ® Rabbit mAb W, IP, IHC-P, IF-F M, R#3520 Phospho-NPM (Ser4) (D19C1) XP ® Rabbit mAb W, F H, (M, R, Mk, X,B, Dg)#3924 NSF (D31C7) XP ® Rabbit mAb W, IP, IF-F H, M, R, Mk#3960 Nur77 (D63C5) XP ® Rabbit mAb W, IP, IF-IC, F H, (Mk)#3686 p27 Kip1 (D69C12) XP ® Rabbit mAb W, IP, IF-IC H, R, Mk #2552New #8238 p75NTR (D4B3) XP ® Rabbit mAb W, IP, IF-IC H, M, R #2693, #4201#5625 Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H, MkNew #9148 Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb IF-IC, FH, Mk(Alexa Fluor ® 488 Conjugate)New #6894 Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb(Alexa Fluor ® 555 Conjugate)IF-ICH, MkABCEventsNF-κB p65NF-κB p65 (D14E12) XP ® Rabbit mAb #8242: Flow cytometric analysis of HeLa cells (A) using #8242 (blue) compared to concentrationmatched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (red). Confocal IF analysis of HT-1080 cells, untreated (B) or treated with hTNF-α#8902 (20 ng/ml, 20 min) (C), using #8242 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ®#4084 (fluorescent DNA dye).8Applications Key: W Western / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F FlowCytometry / ChIP Chromatin Immunoprecipitation / (-IC Immunocytochemistry, -P Paraffin, -F Frozen) / E-P Peptide ELISA

XP ® Monoclonal AntibodySuggested toApplications Reactivity Replace AntibodiesNew #6987 Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb FH, Mk(Alexa Fluor ® 647 Conjugate)New #9185 Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb WH, Mk(Biotinylated)New #8978 Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb FH, Mk(PE Conjugate)New #5838 Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb IPH, Mk(Sepharose Bead Conjugate)New #8190 α-Parvin (D7F9) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk, Dg #4026#9535 PDCD4 (D29C6) XP ® Rabbit mAb W, IP, IHC-P, IF-IC H, M, R#5241 PDGF Receptor α (D13C6) XP ® Rabbit mAb W, IHC-P, IF-IC, F H #3164New #5278 PDGF Receptor α (D13C6) XP ® Rabbit mAb WH(Biotinylated)#3174 PDGF Receptor α (D1E1E) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M#5679 Pdx1 (D59H3) XP ® Rabbit mAb W, IP, IF-IC R, (H) #2437#9349 Perilipin (D1D8) XP ® Rabbit mAb W, IP, IHC-P, IF-F, H, M #3467, #3470IF-IC#3934 PHF20 (D96F6) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (B)#3550 PIAS1 (D33A7) XP ® Rabbit mAb W, IF-IC, F H, M, R, Mk#4730 Pim-2 (D1D2) XP ® Rabbit mAb W, IP, IHC-P, IF-IC H#4053 PKM2 (D78A4) XP ® Rabbit mAb W, IP, IHC-P, IF-IC H, M, R, Mk #3198#5690 PLCγ1 (D9H10) XP ® Rabbit mAb W, IP, IHC-P H, M, R, Mk #2822#3549 PP2C-α (D18C10) XP ® Rabbit mAb W, IP, IHC-P, IF-IC, F H, Mk#2691 PRAS40 (D23C7) XP ® Rabbit mAb W, IP, IHC-P H, M, R, Mk #2610#3450 PSD95 (D27E11) XP ® Rabbit mAb W, IF-F H, M, R #2507#3409 PSD95 (D74D3) XP ® Rabbit mAb W, IHC-P H, M, R #2507#9188 PTEN (D4.3) XP ® Rabbit mAb W, IP, IHC-P H, M, R, Mk #9552, #9559#4005 PTEN (D4.3) XP ® Rabbit mAb (Biotinylated) W, IP H, M, R, Hm, Mk#4326 PTEN (D4.3) XP ® Rabbit mAbIPH, M, R, Mk(Sepharose Bead Conjugate)New #9893 PZR (D17B10) XP ® Rabbit mAb W, IP, IF-IC H#9367 Rab7 (D95F2) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #2094New #6975 Rab8 (D22D8) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #4637#5118 Rab9 (D52G8) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #2095New #8127 Rab10 (D36C4) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #4262#5589 Rab11 (D4F5) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #3539#3900 Rabbit (DA1E) mAb IgG XP ® Isotype Control IHC-P, IF-IC, F, ChIP#2975 Rabbit (DA1E) mAb IgG XP ® Isotype Control F #4340(Alexa Fluor ® 488 Conjugate)#2985 Rabbit (DA1E) mAb IgG XP ® Isotype Control F #3452(Alexa Fluor ® 647 Conjugate)#4096 Rabbit (DA1E) mAb IgG XP ® Isotype Control IP, F(Biotinylated)New #8726 Rabbit (DA1E) mAb IgG XP ® Isotype Control IP(Magnetic Bead Conjugate)#5742 Rabbit (DA1E) mAb IgG XP ® Isotype Control F(PE Conjugate)#3423 Rabbit (DA1E) mAb IgG XP ® Isotype Control IP(Sepharose Bead Conjugate)#5466 RagC (D31G9) XP ® Rabbit mAb W, IP, IHC-P H, M, R, MkReactivity Key: H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish /B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology9

XP ® Monoclonal AntibodySuggested toApplications Reactivity Replace Antibodies#5025 Phospho-RelB (Ser552) (D41B9) XP ® Rabbit mAb W, IP, IF-IC, F H, M, (R, Mk, B, Dg) #4999#3669 p115 RhoGEF (D25D2) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk#9538 Ribosomal Protein S3 (D50G7) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk #2579#5694 RING1B (D22F2) XP ® Rabbit mAb W, IP, IF-IC, ChIP H, M, R, Mk#3493 RIP (D94C12) XP ® Rabbit mAb W, IP, IF-IC, F H, M, R, Hm, Mk #4926New #5528 RSK2 (D21B2) XP ® Rabbit mAb W, IF-IC, F H, M, R, Mk, B, Pg,(Dg)#9340#4858 Phospho-S6 Ribosomal Protein (Ser235/236)(D57.2.2E) XP ® Rabbit mAb#4803 Phospho-S6 Ribosomal Protein(Ser235/236) (D57.2.2E) XP ® Rabbit mAb(Alexa Fluor ® 488 Conjugate)#3985 Phospho-S6 Ribosomal Protein(Ser235/236) (D57.2.2E) XP ® Rabbit mAb(Alexa Fluor ® 555 Conjugate)#4851 Phospho-S6 Ribosomal Protein(Ser235/236) (D57.2.2E) XP ® Rabbit mAb(Alexa Fluor ® 647 Conjugate)#3945 Phospho-S6 Ribosomal Protein (Ser235/236)(D57.2.2E) XP ® Rabbit mAb (Biotinylated)#5316 Phospho-S6 Ribosomal Protein (Ser235/236)(D57.2.2E) XP ® Rabbit mAb (PE Conjugate)#5364 Phospho-S6 Ribosomal Protein(Ser240/244) (D68F8) XP ® Rabbit mAb#5018 Phospho-S6 Ribosomal Protein(Ser240/244) (D68F8) XP ® Rabbit mAb(Alexa Fluor ® 488 Conjugate)New #5044 Phospho-S6 Ribosomal Protein(Ser240/244) (D68F8) XP ® Rabbit mAb(Alexa Fluor ® 647 Conjugate)W, IHC-P, IHC-F,IF-IC, FH, M, R, Mk, Sc, (C) #2211, #4856,#4857IF-IC, F H, M, R, Mk, Sc, (C) #4854IF-ICIF-IC, IF-P, FH, M, R, Mk, Sc, (C)H, M, R, Mk, Sc, (C)W, F H, M, R, Mk, Sc, (C)FH, M, R, Mk, Sc, (C)W, IP, IHC-P, IF-IC, F H, M, R, Mk #2215IF-IC, FFH, M, R, MkH, M, R, Mk#5591 SENP3 (D20A10) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk#5445 Phospho-SHIP2 (Tyr1135) (D33C6) XP ® Rabbit mAb W, IP, F H #2007#5439 SKAR α/β (D65E8) XP ® Rabbit mAb W, IF-IC H, M, R, Mk #3794New #6944 Smad1 (D59D7) XP ® Rabbit mAb W, IP, IF-IC, F, ChIP H, M, (Mk, X, B) #9743#9510 Phospho-Smad2 (Ser465/467)/ Smad3IF-IC, FH(Ser423/425) (D6G10) XP ® Rabbit mAb#5339 Smad2 (D43B4) XP ® Rabbit mAb W, IP, IF-IC, F, ChIP H, M, R, Mk #3103, #3122#3579 Sox2 (D6D9) XP ® Rabbit mAb W, IHC-P, IF-IC, F H, (Mk, B, Dg) #2748New #5049 Sox2 (D6D9) XP ® Rabbit mAbIF-IC, FH, (Mk, B, Dg)(Alexa Fluor ® 488 Conjugate)#5179 Sox2 (D6D9) XP ® Rabbit mAbIF-ICH, (Mk, B, Dg)(Alexa Fluor ® 555 Conjugate)#5067 Sox2 (D6D9) XP ® Rabbit mAbIF-IC, FH, (Mk, B, Dg)(Alexa Fluor ® 647 Conjugate)#5024 Sox2 (D6D9) XP ® Rabbit mAb (ChIP Formulated) ChIP H, (Mk, B, Dg)#5147 SRF (D71A9) XP ® Rabbit mAb W, IP, ChIP H, M, R, Mk, PgNew #5882 STAG2 (D25A4) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (C, #4239B, Pg)#9145 Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb W, IP, IHC-P, IHC-F, H, M, R, Mk #9131IF-IC, F, ChIP#4323 Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb(Alexa Fluor ® 488 Conjugate)FH, M, R, Mk10Applications Key: W Western / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F FlowCytometry / ChIP Chromatin Immunoprecipitation / (-IC Immunocytochemistry, -P Paraffin, -F Frozen) / E-P Peptide ELISA

XP ® Monoclonal Antibody Applications Reactivity#4324 Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb FH, M, R, Mk(Alexa Fluor ® 647 Conjugate)#4093 Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb WH, M, R, Mk(Biotinylated)New #8119 Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb FH, M, R, Mk(PE Conjugate)#4074 Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb IPH, M, R, Mk(Sepharose Bead Conjugate)#4322 Phospho-Stat5 (Tyr694) (D47E7) XP ® Rabbit mAb W, IP, IF-IC, F H, M, (R, Mk, B)New #5659 Nonphospho-STEP (Ser221) (D74H3) XP ®W, IP, IF-F H, M, RRabbit mAb#3737 SUZ12 (D39F6) XP ® Rabbit mAb W, IP, IF-IC, ChIP H, M, R, MkSuggested toReplace AntibodiesSignal relative to input0.0500.0450.0400.0350.0300.0250.0200.0150.0100.0050SUZ12 (D39F6) XP ® Rabbit mAb #3737Normal Rabbit IgG #2729HoxA1 Hoxa2 α SateliteSUZ12 (D39F6) XP ® Rabbit mAb #3737: Chromatin immunoprecipitationswere performed with cross-linked chromatin from 4 x 10 6 NCCIT cells andeither 5 µl of #3737 or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA wasquantified by real-time PCR using human HoxA1 intron 1 primers, SimpleChIP ®Human HoxA2 Promoter Primers #5517, and SimpleChIP ® Human α SatelliteRepeat Primers #4486. The amount of immunoprecipitated DNA in eachsample is represented as signal relative to the total amount of input chromatin(equivalent to one).#5297 Synapsin-1 (D12G5) XP ® Rabbit mAb W, IP, IHC-P, IF-F H, M, R #2312#5461 Synaptophysin (D35E4) XP ® Rabbit mAb W, IP, IF-F H, M, R #4329#4179 α-Synuclein (D37A6) XP ® Rabbit mAb W, IP, IHC-P, IF-F M, RNew #8842 Phospho-TACC3 (Ser558) (D8H10) XP ® Rabbit mAb W, IF-IC HNew #8069 TACC3 (D9E4) XP ® Rabbit mAb W, IP, IF-IC H#5483 Phospho-TBK1/NAK (Ser172)(D52C2) XP ® Rabbit mAbW, IP, IF-IC, F H, (M, R, Mk, X,B, Dg)#3557 TGM2 (D11A6) XP ® Rabbit mAb W, IHC-P, IF-IC H, M, R, MkNew #8509 TIAR (D32D3) XP ® Rabbit mAb W, IP, IF-IC, F H, M, R, Mk #5137, #8611#4308 Tuberin/TSC2 (D93F12) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Hm, Mk #3612, #3635#5335 Acetyl-α-Tubulin (Lys40) (D20G3) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk, Z#5666 β3-Tubulin (D65A4) XP ® Rabbit mAb W, IP, IHC-P H, M, R#5568 β3-Tubulin (D71G9) XP ® Rabbit mAb W, IP, IF-F H, M, R #4466#4786 Ubc9 (D26F2) XP ® Rabbit mAb W, IF-IC H, M, R, Mk #4918#5741 Vimentin (D21H3) XP ® Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk #3932New #9854 Vimentin (D21H3) XP ® Rabbit mAbIF-IC, FH, M, R, Mk(Alexa Fluor ® 488 Conjugate)New #9855 Vimentin (D21H3) XP ® Rabbit mAbIF-ICH, M, R, Mk(Alexa Fluor ® 555 Conjugate)New #9856 Vimentin (D21H3) XP ® Rabbit mAbIF-IC, FH, M, R, Mk(Alexa Fluor ® 647 Conjugate)#3659 WAVE-2 (D2C8) XP ® Rabbit mAb W, IP, IF-IC H, M, R, Mk#3165 Zap-70 (D1C10E) XP ® Rabbit mAb W, IP, IF-F, F H, M #2701New #9473 Zap-70 (D1C10E) XP ® Rabbit mAbFH, M(Alexa Fluor ® 488 Conjugate)#3704 ZO-3 (D57G7) XP ® Rabbit mAb W, IF-IC HReactivity Key: H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish /B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology11

USA HeadquartersCell Signaling TechnologyTechnical Support: (toll-free) 1-877-678-8324Tel: 978-867-2300 / Fax: 978-867-2400E-mail: info@cellsignal.com / www.cellsignal.comIn the U.S. for Bulk Volumes or Pricing Information,contact our sales team: sales@cellsignal.comInternational SubsidiariesCell Signaling Technology (China) LimitedTechnical Support: (toll-free) 4006 GreatQ (473287)Tel: (86) 21-5835-6288 / Fax: (86) 21-5835-6116E-mail: info@cst-c.com.cn / www.cellsignal.comCell Signaling Technology Japan, K.K.Tel: 03 (5652) 0213 / Fax: 03 (3249) 1170E-mail: info@cstj.co.jp / www.cstj.co.jpCell Signaling Technology EuropeTel: +31 (0)71 568 1060 / Fax: +31 (0)71 568 1065E-mail: info@cellsignal.eu / www.cellsignal.comANTIBODIES AND RELATED REAGENTSFOR SIGNAL TRANSDUCTION RESEARCHPrinted in the USA on recycled paper using soy inks and processed chlorine free.© 09/2011 Cell Signaling Technology, Inc.Cell Signaling Technology ® , XP ® , XMT ® , eXceptional Performance , SimpleChIP ® , and CST are trademarks of Cell Signaling Technology, Inc. DRAQ5 ® is a trademark of Biostatus Limited. Alexa Fluor ® is a trademark ofMolecular Probes, Inc. Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U.S. Patents No. 5,675,063).All content of this Brochure and Technical Reference is protected by U.S. and foreign intellectual property laws. You may not copy, modify, upload, download, post, transmit, republish or distribute any of the content withoutour prior written permission except for your own personal and non-commercial purposes. Except as provided in the preceding sentence, nothing contained in this Brochure and Technical Reference shall be construed asgranting a license or other rights under any patent, trademark, copyright or other intellectual property of Cell Signaling Technology or any third party. Unauthorized use of any Cell Signaling Technology trademark, servicemark or logo may be a violation of federal and state trademark laws.