miRNA Analysis - OriGene

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Verify the GFP expression Mir205 Mir143 Mir34b Empty Vector Non-transfected 24hr after transfection
Demonstrate the over-expression MicroRNA vector untransfected Method: HEK293 cells were transfected with <strong>miRNA</strong> expression plasmids and the total RNA were isolated 48 hrs later. <strong>miRNA</strong> were detected using Poly A tailing RT_PCR. All experiments were conducted in duplicates. Beta-actin were used as normalization control. Mir205 Beta-actin Mir143 Beta-actin Mir34b Beta-actin 24hr after transfection
Page 1 and 2: OriGene Technologies, Inc. MicroRNA
Page 3 and 4: Optimal strategies to a successful
Page 5 and 6: miRNAs regeulate of gene expression
Page 9 and 10: Hairpin primer Poly A tailing
Page 11 and 12: qSTAR qPCR microRNA detection Syste
Page 13 and 14: miRNA template standards Copy numbe
Page 15 and 16: miRNA expression perturbation Pre-m
Page 17: Validation of pCMV6-MIR system 1. S
Page 23 and 24: Luciferase 3’ UTR reporter system
Page 25 and 26: Why introduce IRES? pSV40-GFP pSV40
Page 27 and 28: pMir-Target system validation -Inte
Page 29 and 30: Percentage of control pMir-Target s

miRNA Analysis - OriGene

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