miRNA Analysis - OriGene
miRNA Analysis - OriGene
miRNA Analysis - OriGene
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Comparison of different luciferase<br />
reporter assays<br />
pMIR-Report<br />
(Ambion)<br />
Dual-Luciferase<br />
reporter<br />
(Promega)<br />
Sensitivity low low high<br />
pMir_Target (<strong>OriGene</strong>)<br />
Reporter firefly luciferase firefly luciferase firefly luciferase<br />
Promoter CMV (strong) PGK (weaker) SV40 (strong)<br />
Transfection marker and<br />
internal control<br />
non<br />
SV40 drives second<br />
luciferase<br />
CMV drives RFP<br />
Methods to weaken reporter<br />
expression<br />
Compatibility with <strong>OriGene</strong>’s<br />
pMir <strong>miRNA</strong> system<br />
non Weaker promoter IRES weakening the<br />
translation<br />
yes yes yes<br />
Stable transfection no no yes
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