miRNA Analysis - OriGene

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<strong>OriGene</strong>’s design works SV40 P Neo stop IRES GFP stop SV40 P Neo stop IRES GFP stop CMV P CMV P mir205 precursor Empty reporter pCMV-Mir Empty reporter Mir205 SV40 P CMV P Neo stop IRES GFP stop Mir205 target SV40 P CMV P stop Neo IRES mir205 precursor GFP stop Mir205 target Mir205 target pCMV-Mir Mir205 target Mir205
pMir-Target system validation -Interaction of mir205 with its target genes Percentage of control Table 1. 3’UTR-luciferase reporter clones in mir205 targets study Sample number 1 3’UTR reporter clones in pMir-Target NM_001982 ** *** ** * ** * ** ** ** 2 3 4 5 6 7 8 NM_030751 NM_001567 NM_014795 NM_001002814 NM_001025376 NM_005433 NM_014962 9 NM_024830 10 NM_019084 11 Mir205 Rev. comp. 12 Empty reporter Sample Number Mir205 down-regulated luciferase activity when the luciferase was fused to mir205 target sequences (grey bar, control with 3’ UTR reporter clones; black bar, mir205 with 3’ UTR reporter clones.)
Page 1 and 2: OriGene Technologies, Inc. MicroRNA
Page 3 and 4: Optimal strategies to a successful
Page 5 and 6: miRNAs regeulate of gene expression
Page 9 and 10: Hairpin primer Poly A tailing
Page 11 and 12: qSTAR qPCR microRNA detection Syste
Page 13 and 14: miRNA template standards Copy numbe
Page 15 and 16: miRNA expression perturbation Pre-m
Page 17 and 18: Validation of pCMV6-MIR system 1. S
Page 19 and 20: Demonstrate the over-expression Mic
Page 23 and 24: Luciferase 3’ UTR reporter system
Page 25: Why introduce IRES? pSV40-GFP pSV40
Page 29 and 30: Percentage of control pMir-Target s

miRNA Analysis - OriGene

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