miRNA Analysis - OriGene

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pMir-Target system validation -Interaction of mir205 with its target genes Seed sequence of mir205 in the 3’ UTR of ERBB3 GC GAU 5’ ACAG ACUCC--UGGA AUGAAGGAU-3’ ||| ||||| |||| |||||||| 3’ GUC--UGAGG ACCU---UACUUCCU- 5’ CC AA Mutate the Seed sequence NM_001982(ERBB3) mir205 Table of experiment set-ups Transfections Control Exp1 Exp2 pMir-Target-3UTR + + pMir-Target-3UTR mutant + MiR in pMir + + PMir(Empty Vector) +
Percentage of control pMir-Target system validation -Interaction of mir205 with its target genes Table of experiment set-ups Transfections Control Exp1 Exp2 pMir-Target-3UTR + + pMir-Target-3UTR mutant + MiR in pMir + + PMir(Empty Vector) + 140 120 100 80 60 40 20 0 NM_001982 NM_030751 NM_001567 NM_014962 Empty Abolishment of Mir205 effects by mutated seeding sequences in the 3’UTR-luciferase reporter clones (grey bar, control with 3’ UTR reporter clones; darker grey bar, mir205 with 3’ UTR reporter clones; black bar, mir205 with mutated 3’ UTR reporter clones.)
Page 1 and 2: OriGene Technologies, Inc. MicroRNA
Page 3 and 4: Optimal strategies to a successful
Page 5 and 6: miRNAs regeulate of gene expression
Page 9 and 10: Hairpin primer Poly A tailing
Page 11 and 12: qSTAR qPCR microRNA detection Syste
Page 13 and 14: miRNA template standards Copy numbe
Page 15 and 16: miRNA expression perturbation Pre-m
Page 17 and 18: Validation of pCMV6-MIR system 1. S
Page 19 and 20: Demonstrate the over-expression Mic
Page 23 and 24: Luciferase 3’ UTR reporter system
Page 25 and 26: Why introduce IRES? pSV40-GFP pSV40
Page 27: pMir-Target system validation -Inte

miRNA Analysis - OriGene

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