miRNA Analysis - OriGene
miRNA Analysis - OriGene
miRNA Analysis - OriGene
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<strong>miRNA</strong> targets analysis<br />
Methods pros cons<br />
Luciferase reporter Easy, Sensitive and reliable Require premade reporter<br />
construct<br />
Western blot Less sensitive, very definitive Most antibodies are not<br />
available<br />
DNA microarray<br />
Whole genome coverage, high<br />
throughput<br />
In silica predication Easy Error-prone<br />
Changes in mRNA level<br />
have poor co-relation to<br />
<strong>miRNA</strong> function<br />
Luciferase reporter assays are the most widely used methods in <strong>miRNA</strong> target<br />
identification
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