miRNA Analysis - OriGene

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Number of miRNA Publications miRNAs regulates many biological processes 3500 3000 2500 2000 1500 1000 500 0 1993- 1998 1998- 2000 2001- 2002 2003- 2004 2005- 2006 2007- 2008 2009- 2010 Year of Publication First miRNA, lin-14 in C. elegans, was reported by Vector Ambros in 1993. Since then miRNA has been reported: 1. Targeting most development related genes 2. stem cells maintenance and differentiation. 3. Cancer as oncomiR. 4. Heart disease. 5. Neuronal development and synapse formation. 6. Viral infections process.
Optimal strategies to a successful miRNA research project miRNA Biology miRNA cDNA synthesis Global miRNA profiling Searching miRNAs involving in your research Specific miRNA detection and quantification miRNA expression perturbation Studying specific miRNAs miRNA targets identification Bridging the gap between miRNA and biology by identifying protein target of miRNA
Page 1 and 2: OriGene Technologies, Inc. MicroRNA
Page 3 and 4: Optimal strategies to a successful
Page 5: miRNAs regeulate of gene expression
Page 9 and 10: Hairpin primer Poly A tailing
Page 11 and 12: qSTAR qPCR microRNA detection Syste
Page 13 and 14: miRNA template standards Copy numbe
Page 15 and 16: miRNA expression perturbation Pre-m
Page 17 and 18: Validation of pCMV6-MIR system 1. S
Page 19 and 20: Demonstrate the over-expression Mic
Page 21 and 22: Optimal strategies to a successful
Page 23 and 24: Luciferase 3’ UTR reporter system
Page 25 and 26: Why introduce IRES? pSV40-GFP pSV40
Page 27 and 28: pMir-Target system validation -Inte
Page 29 and 30: Percentage of control pMir-Target s

miRNA Analysis - OriGene

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