Grace Catalog 2008

Grace Catalog 2008

여백입니다.

여백입니다.

HPLC | IC 

HPLC Instruments . . . . . . . . . . . . . . . . 5–23 

Detectors 5–11, 14 

ELSD 5–11 

UV 14 

Nitrogen Gas Generators 12–13 

for ELSD 12 

for LC/MS 13 

Autosamplers 15 

Column Heaters 16–17 

Pumps 18–21 

Data Collection & Software 22–23 

HPLC Columns . . . . . . . . . . . . . . . . . .24–97 

Introduction 24–33 

Small Molecule 34–80, 82–83 

Grace ® VisionHT 34–37 

Grace ® GraceSmart 50–51 

Grace ® GraceAlpha 52 

Alltech ® Alltima HP 38–43 

Alltech ® Prevail 44–47 

Alltech ® Platinum 48–49 

Alltech ® Brava 53 

Alltech ® Alltima 54–56 

Alltech ® Apollo 57 

Alltech ® Econosphere 57 

Alltech ® Adsorbosphere 58–59 

Alltech ® Allsphere 59 

Alltech ® Specialty Small Molecule 67–68 

OPA Amino Acid, Catecholamine, Nucleotide-Nucleoside, 

Mixed-Mode, Carbohydrate Cation 

Grom Sil 60–61 

Grom Sapphire 62 

Grom Amino Acid Kits 63 

Jones Genesis ® 64–65 

Jones Apex 66 

Vydac ® Venture ® 69 

Vydac ® Denali ® 69 

Vydac ® 201TP and 202TP C18 70–71 

Distributed Small Molecule Columns 72–83 

Astec , Brownlee , Jordi , LiChrosorb ® , LiCrospher ® , Nucleosil ® , 

Partisil , Partisphere ® , Polymer Labs, Shodex ® , Spherisorb ® , 

Ultrasphere ® , ZirChrom ® 

Large Molecule 84–97 

Vydac ® MS 85–87 

Vydac ® Everest ® . . . . . . . . . . . . . . . . . . . . . . 88–89 

Vydac ® TP 90–92 

Vydac ® ProZap C18 93–94 

Vydac ® Venture ® A 95 

Alltech ® ProSphere 96–97 

Alltech ® Macrosphere 97 

Distributed Large Molecule Columns 74–75, 79–81 

Brownlee , Jordi , Polymer Labs, Shodex ® , 

IC Columns . . . . . . . . . . . . . . . . . . . 98–105 

Custom HPLC Columns . . . . . . . . . . . . . . 106 

Empty HPLC Hardware, Frits. . . . . . . . . 107–110 

HPLC Accessories . . . . . . . . . . . . . . 111–146 

Prefilters 111 

Fittings 112–122 

Mobile Phase Handling 123–131 

Pump Accessories 132–135 

Valves 136–146

analytical hplc 

Evaporative Light Scattering Detectors 

Leading the Way in ELSD Technology for More Than 20 Years 

Alltech has more than 20 years of experience engineering and manufacturing Evaporative Light Scattering Detectors. Our global 

network of dedicated application chemists and technical support professionals provides you with the comprehensive service and 

support you need. 

How Does It Work? 

hplc instruments 

ELSDs detect any compound less volatile than the mobile phase using a simple three-step process: 

1. Nebulization 

7049 

2. Evaporation 

7048 

3. Detection 

7047 

Column effluent passes 

through a needle and mixes 

with nitrogen gas to form a 

dispersion of droplets. 

Droplets pass through a heated 

“drift tube” where the mobile 

phase evaporates, leaving a fine 

mist of dried sample particles in 

solvent vapor. 

The sample particles pass 

through a cell and scatter light 

from a laser beam. The scattered 

light is detected, generating 

a signal. 

Are There Any Limitations? 

1. The mobile phase must be volatile. 

2. The sample must be less volatile than the mobile phase. 

ELSD Advantages Over Other LC Detectors 

ELSD RI UV MS 

CHART KEY 

Sensitivity 

Gradient Capability 

Baseline Stability 

Solvent Interference 

Mass Balance 

Excellent: 

Good: 

Poor: 

more info 

For more information about Evaporative Light Scattering 

Detection, including applications, literature, and an informational 

movie, visit www.elsd.com. 

www.discoverysciences.com



ELSD Advantages Over Other LC Detectors 

ELSD improves baseline stability and detection sensitivity 

compared to refractive index (RI). 

Solvent 

Front 

Sugar Alcohols 

1 

1 

2 

3 

2 3 

ELSD 

RI 

10627 

10635 

1. Xylitol (200ppm) 

2. Sorbitol (200ppm) 

3. Dulcitol (200ppm) 

ELSD shows what may be missing from your UV 

chromatogram. 

Plant Oil Extract 

0 10 20 30 40 50 60 

ELSD 

10656 

10657 

UV at 254nm 

70 Min. 

0 2 4 6 8 10 12 14 16 

18 Min. 

Column: Alltech ® Prevail Carbohydrate ES, 5µm, 

4.6 x 250mm (Part No. 35101) 

Mobile Phase: Acetonitrile:Water (79:21) 

Flow Rate: 1.0mL/min 

Column Temp: 30°C 

Column: Alltech ® Alltima C18, 3μm, 2.1 x 150mm 

(Part No. 43852) 

Mobile Phase: A: 0.1% Formic Acid B: Methanol 

Gradient: Time: 0 40 

%B: 60 100 



Use ELSD in parallel with UV/Vis and MS for maximum 

structural and concentration information. 

1 

Steroid Hormone Formulation 

1. Povidone (8 µg) 

2. Chlormadinone Acetate (1.5 µg) 

3. Mestranol (3 µg) 

2 

2 

3 

2 

ELSD 

1 

3 

MS 

1 

3 

UV at 254nm 

0 2 4 6 8 10 12 14 16 18 20 Min. 

10639 

10641 

10640 

Column: Vydac ® Denali ® 238DE C18, 3µm, 2.1 x 50mm 

(Part No. 238DE3205) 

Mobile Phase: 0.2% Formic Acid in Acetonitrile:Water (55:45) 


Column Temp: Ambient 

ELSD obtains a more accurate representation of sample 

mass than UV/Vis. 

1. Mephobarbital (500ppm) 

2. Secobarbital (500ppm) 

1:1 Mixture 

UV at 230nm 

0 2 4 6 8 10 12 14 16 18 20 Min. 

1 

2 

1 

2 

ELSD 

10616 

10617 

Column: Alltech ® Alltima HP C18 HL, 5µm, 4.6 x 250mm 

(Part No. 87698) 




technical assistance 

Contact Tech Support: Phone: 1.800.255.8324 (North America) 

Email: contact.alltech@grace.com 

Online: www.discoverysciences.com 

more applications 

To view our complete searchable 

chromatogram database visit 

www.discoverysciences.com/chromdb/ 

 



Alltech ® 3300 ELSD 

High Sensitivity and Ease of Use 

Detection of non-volatile compounds has never been easier 

than with our new, most sensitive ELSD. 

Sensitive 

• Low noise and excellent baseline stability 

• Low nanogram detection limits 

Simple 

• Step-by-step method wizard automates detector setup 

• Easy access to parts requiring cleaning or maintenance 

Small 

• Small footprint minimizes bench space requirements 

11.6" H x 10.3" W x 19.5" D (29.5cm H x 26.2cm W x 49.5cm D) 


6947b 

A Windows ® -based 

user interface makes 

navigating through 

menus intuitive and 

easy. Drivers for Agilent 

ChemStation and 

EZChrom Elite/EZStart 

are available. 

6967 

Alltech ® 3300 ELSD* 

Description 

Part No. 

3300 ELSD 110V–240V 5135834 

*Patent pending. 

Service and Support 

Our dedicated technical support professionals provide you 

with the comprehensive support you need. Our complete line 

of service products, including qualification, installation, training, 

repair service, and preventive maintenance, lets you choose 

what best meets your needs and budget requirements. 

Services for the Alltech ® 3300 ELSD 

Description 

Installation and Training 

Qualification 

IQ/OQ at Customer Site 

PQ at Customer Site 

IQ/OQ/PQ at Customer Site 

Part No. 

INSTALL 

Q330010 

Q330020 

Q330030 

Alltech ® ELSDs are designed and manufactured in 

accordance with the following standards: EN6101-1 + A2:95, 

EN555011: 1998, FCC CFR 47 Part 15: 2001, 

EN 61326: 1998, EN 6100-3-2: 1995 + A14:2000 and 

EN61000-3-3: 1995 

3300 ELSD Specifications 

Light Source: 

Detector Element: 

Temperature Range: 

Nebulizer Gas: 

Inlet Pressure: 

Typical Operating Range: 

Flow Control: 

Mobile Phase Flow Rate: 

Analog Output: 

Communications: 

User Interface: 

Power Requirements: 

Dimensions: 

Weight: 

IEC/FDA Classification: 

Manufacturing Standards: 

Warranty: 

Control: 

Laser diode with collimating optics, 650nm, 

30mW output, class IIIB 

Silicon photodiode 

Ambient to 120ºC in 1ºC increments 

Nitrogen preferred; Calibrated to 4.0L/min 

60–80psig 

1.0–2.0L/min 

Digital Mass Flow Control 

To 3.0mL/min 

1V or 10mV full scale 

Inputs: TTL/contact closure for auto zero, 

gas shutoff, start, and standby 

Outputs: Contact Closure for pump 

shutdown in error conditions, general events 

Data Ports: USB, RS232, Ethernet 

Full-color Microsoft Windows ® -based 

Graphical LCD with alphanumeric keypad 

120/240V, 50/60Hz 

11.6" H x 10.3" W x 19.5" D 

(29.5cm H x 26.2cm W x 49.5cm D) 

30lb (16kg) 

Class 1 Laser Product; Complies with 

21 CFR Subpart J 

Meets all applicable safety and EMC 

certifications; CE, UL, and CSA certified 

1 year parts and labor 

Drivers for Agilent Chemstation and 

EZChrom Elite/EZ Start 




ELSD Sensitivity and Reproducibility 

With reduced noise and greater baseline stability, the 3300 ELSD is more sensitive than any other ELSD available. 

2 

2 

1 

2 

3 

3 

2 

3 

3 

3 

Competitor A 

Competitor B 

Competitor C 

1. Methyl Paraben (200ng) 

2. Propyl Paraben (200ng) 

3. Butyl Paraben (200ng) 

Model 

Butyl Paraben 

Limit of Detection (LOD) 

3300 ELSD 25ng 

Competitor A 200ng 

Competitor B 200ng 

Competitor C 150ng 

Competitor D 50ng 

Competitor D 

1 

3300 ELSD 

0 1 2 3 4 5 6 7 8 Min. 

Column: Alltech ® Alltima C18, 5µm, 4.6 x 250mm 

(Part No. 88056) 

Mobile Phase: Water:Acetonitrile (40:60) 



The 3300 ELSD’s near ambient temperature evaporation 

maximizes sensitivity for semi-volatiles. 

The 3300 ELSD is very reproducible as demonstrated by 

10 identical injections of four ginkgolide components. 

1 

1 

1. Ibuprofen (100ppm) 

Component % RSD 

1. Bilobalide 1.7 

2. Ginkgolide C 1.3 

3. Ginkgolide A 1.6 

4. Ginkgolide B 1.0 

1 

1 

2 

1 

30°C 

3 

4 

32.5°C 

1 

35°C 

37.5°C 

1 

40°C 

42.5°C 

0 1 

2 3 Min. 

0 2 4 6 8 10 12 14 16 Min. 

Column: Alltech ® Alltima HP C18, 3µm, 2.1 x 100mm 

(Part No. 87669) 

Mobile Phase: Acetonitrile: 0.1%TFA in Water (80:20) 



Column: Alltech ® Platinum C18, 5µm, 4.6 x 250mm 

(Part No. 32064) 

Mobile Phase: Water:Methanol:THF (65:25:10) 







 



ELSD Performance Benefits 

Stable Baselines with Fast, Steep Gradients 

1 

0 1 2 3 Min. 

2 

3 

10658 

1. Acetylsalicylic Acid 

2. Diphenhydramine 

3. Trimipramine 

Use ELSD to Trigger Collection of UV 

Transparent Compounds in Prep Applications 

10679 

1. LLLn 

2 3 

2. LLL 

3. LLO 

4. PLL 

4 5. LOO 

5 6. PLO 

6 7. PLP 

7,8 8. OOO 

1 

9,10 

9. SLO 

10. POO 

0 5 10 15 20 Min. 


Column: Alltech ® Alltima HP C18, 3µm, 

7 x 33mm Rocket (Part No. 87671) 

Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile 


%B: 10 90 



Simplify Detection of Weak Absorbing Polymers 

Column: Vydac ® Denali ® 238DE C18, 10µm, 22 x 150mm 

(Part No. 238DE102215) 

Mobile Phase: A: Acetonitrile B: Dichloromethane 

Gradient: Time: 0 10 20 21 

%B: 45 50 70 45 

Flow Rate: 10mL/min 


Easily Detect Non-Chromophoric Drug Compounds 

1 

10636 

10680 

1. mPEG-SBA 

1 

1. Spectinomycin 

0 3 6 9 12 15 Min. 

Column: Alltech ® ProSphere 300, 5µm, 4.6 x 150mm 

(Part No. 35560) 

Mobile Phase: A: Water B: Acetonitrile 

Gradient: Time: 0 3 15 

%B: 30 30 80 



0 1 

2 3 4 5 6 7 8 9 Min. 

Column: Alltech ® Alltima HP C18 Amide, 3µm, 4.6 x 50mm 

(Part No. 87829) 

Mobile Phase: 10mM Ammonium Carbonate:Acetonitrile (98:2) 



Use ELSD to Simplify Triglyceride Analysis 

600 

500 

400 

300 

200 

100 

0 

0 5 10 

1 

Sesame Seed Oil 

2 

3 

4 

5 7 8 9 

6 

10 

15 20 25 30 35 Min. 

1. LLL 

2. LLO 

3. LLP 

4. OOL 

5. POL 

6. PPL 

7. OOO 

8. OOP 

9. PPO 

10. OOS 

Column: Alltech ® Alltima HP C18 Hi-Load, 5µm, 

4.6 x 250mm (Part No. 87698) 


Gradient: Time: 0 25 26 27 28 

%B: 30 51 70 70 30 



10581 

5 6 

Improve Sensitivity for Carbohydrates 

Robitussin ® Flu Syrup 

10557 

1. Sucralose 

2 4 

2. Fructose 

3. Sorbitol 

4. Glucose 

5. Sucrose 

3 

6. Maltose 

1 

0 5 10 15 20 25 30 35 40 Min. 


4.6 x 250mm (Part No. 35101) 

Mobile Phase: A: Acetonitrile B: Water 


%B: 15 15 25 25 50 





ELSD Performance Benefits 


Use ELSD for Triggering Fraction Collection of UV/Vis 

Transparent Compounds in Preparative Applications 

1 

10438 

1. Ethinamate (5.0 mg/mL) 

Improve Sensitivity for Carbohydrates 

Carbohydrates–10ng on Column 

2 

1 3 

1. Fructose 

2. Sucrose 

3. Raffinose 

10498 

0 

UV 

ELSD 

1 2 3 4 5 6 7 Min. 

Column: Alltech ® Alltima C18, 5µm, 10 x 150mm 

(Part No. 81102) 




0 1 2 3 4 5 6 7 8 9 10 Min. 

Column: Alltech ® Prevail Carbohydrate ES, 5µm, 4.6 x 150mm 

(Part No. 35102) 



%B: 80 50 80 80 



Simplify Detection of Difficult Samples 

PEG 600 

10276 

Detect Difficult Samples without Derivatizing 

Biodiesel Derived from Soy Bean Oil 

10644 

1. Mono-acylglycerol (MAG) 

2. MAG 

3. MAG 

4. Fatty Acid Methyl Ester (FAME) 

5. MAG 

6. FAME 

7. FAME 

8. MAG 

9. FAME 

10. Di-acylglycerol (DAG) 

11. Tri-acylglycerol (TAG) 

4 6 

7 

9 

8 

3 5 

12 10 

11 

0 3 6 9 12 15 Min. 

Column: Alltech ® Adsorbosphere C8, 5µm, 4.6 x 150mm 

(Part No. 287122) 

Mobile Phase: A: Water B: Methanol 


%B: 30 60 


0 5 10 15 20 25 30 Min. 

Column: Alltech ® Alltima HP C18 HiLoad, 

5µm, 4.6 x 250mm (Part No. 87698) 



%B: 0 15 70 70 0 


10 

www.discoverysciences.com 

more info 

Interested in ELSD for biodiesel process monitoring? We 

have a complete solution including equipment, columns, and 

accessories. Request sheet M181 or call or visit our website for 

more information.


Alltech ® ELSD 800 

The Benefits of ELSD for 

the Price of a UV/VIS or 

RI Detector 

Low Cost, High Performance 

The ELSD 800 is an economical alternative to 

full-featured ELSDs. Now everyone can afford to add 

universal HPLC detection to any HPLC system to replace 

or complement existing detectors. 

Easy to Use 

The ELSD 800 is easy to operate and requires virtually no 

optimization. The same low temperature and gas flow is used 

for most applications, including organic and aqueous mobile 

phases, and gradient methods. 

Small and Stackable 

ELSD 800 Specifications 

Light Source: 

Laser diode with collimating optics, 670nm, 

max output less than 5mW, class IIIA 

Detector Element: Silicon photodiode 

Temperature Range: Ambient to 110°C in 1°C increments 

Nebulizer Gas: 

Up to 3.0L/min, nitrogen recommended 

Inlet Pressure: 

15–90psig (1.0–6.0 Bar) 

Typical Operating Range: 1.0–3.0L/min 

Flow Control: 

Fixed orifice with regulated pressure 

Mobile Phase Flow Rate: To 5.0mL/min 

Analog Output: 

1V or 10mV full scale 

Communications: Outputs: Contact closure for pump shutdown 

in error conditions 

User Interface: 

Liquid crystal display and keypad entry of 

operating parameters 

Power Requirements: 120/240V, 50/60Hz 

Dimensions: 

14.0" H x 13.0" W x 18.0" D 

(35.6cm H x 33.1cm W x 45.7cm D) 

Weight: 

20lb (9kg) 

Warranty: 

1 year parts and labor 

*Patent Pending. 

Alltech ® ELSD 800 

Description 

Part No. 

ELSD 800 

120V 800100 

240V 800200 

6419KO 

The ELSD 800 is small and stackable, minimizing bench space 

needed, especially when using multiple ELSDs in close proximity. 

ELSD Qualification 

and Service 

Service and Support 

Our dedicated technical support professionals provide you 

with the comprehensive support you need. Our complete line 

of service products, including qualification, installation, training, 

repair service, and preventive maintenance, lets you choose 

what best meets your needs and budget requirements. 

Qualification (IQ/OQ/PQ) 

Grace makes it easy for you to integrate the ELSD into 

a qualified HPLC system. Detailed IQ/OQ/PQ procedures are 

included with the 3300 ELSD to help you perform your own 

qualification. We also provide the option of on-site qualification. 

Qualification Services 

Description 

3300 ELSD 

IQ/OQ at customer site 

PQ at customer site 

IQ/OQ/PQ at customer site 

Re-OQ performed on site 

Re-OQ/PQ performed on site 

800 ELSD 

IQ/OQ performed on site 

PQ performed on site 

IQ/OQ/PQ performed on site 



2000ES ELSD 



2000 ELSD 



Preventive Maintenance and Repair 

Part No. 

Q330010 

Q330020 

Q330030 

Q330040 

Q330050 

Q80010 

Q80020 

Q80030 

Q80040 

Q80050 

Q60040ES 

Q60050ES 

Q60040 

Q60050 

Grace offers many flexible options for maintenance and 

repair of your ELSD. These services can be performed at 

your site or at our facility. Service contracts are available. An 

annual cleaning and calibration is recommended. Extended 

warranties can be purchased to provide peace of mind and/or 

extend the warranty on refurbished instruments. 

Preventive Maintenance 

Description 

Part No. 

Preventive Maintenance Visit – Includes replacement of 600365 

nebulizer, light trap, o-rings, and apertures 

Cleaning and NIST Calibration at Grace 

500 ELSD 500361 

800 ELSD 800361 

2000/2000ES ELSD 600361 

3300 ELSD 3300361 

Cleaning and NIST Calibration at Customer Site 

500 ELSD 500363 

800 ELSD 800363 

2000/2000ES ELSD 600363 

3300 ELSD 3300363 

ELSD Service Agreement – 1-year repair, parts, and labor at 

Grace repair center, includes annual clean + NIST calibration 

800, 2000/2000ES 600370 

3300 3300370 


www.discoverysciences.com 11



ELSD Nitrogen Generator 

• Simplifies ELSD operation 

• Produces a continuous supply of high purity nitrogen 

from compressed air 

• Replaces inconvenient nitrogen cylinders 

• Requires no electricity 

Water vapor and oxygen permeate through the wall of the 

membrane quickly. Only nitrogen flows through the fiber bores 

to the ELSD. 

The ELSD Nitrogen Generator has a small, compact footprint that 

fits easily on the lab bench next to your ELSD or mounts on the 

wall nearby. 

Increase productivity, reduce operating costs, and improve 

lab safety by eliminating nitrogen cylinders. Forget about the 

need to change the nitrogen cylinder or wondering whether 

there is enough nitrogen left to finish the run. 

The ELSD Nitrogen Generator requires no electricity and has 

no moving parts. Nitrogen is generated using a proprietary 

membrane separation technology. Simply connect the 

generator to your lab’s existing compressed air supply or 

suitable air compressor. The generator separates air into its 

component gases and only nitrogen flows through to the ELSD. 

The ELSD Nitrogen Generator produces a continuous, on 

demand supply of pure nitrogen at flow rates up to 4L/min. 

Two ELSDs may be used with one generator as long as your 

total gas flow rate is less than 4L/min. 

more info 

For information on air compressors, see page 211. 

6053 

Nitrogen Generator Specifications 

Nitrogen Purity: 99% 

Nitrogen Flow: 

4L/min 

Suspended Liquids: None 

Particles: 


LC/MS Nitrogen Generators 

High Purity Nitrogen from Parker and Domnick Hunter 

• Choose from membrane and PSA technologies 

• Stand-alone units or models with built-in compressor 

• Wide range of flows to meet your needs 

LC/MS instrumentation uses nitrogen as purge gas in its nebulizer, and has special nitrogen purity requirements. Parker Balston ® 

nitrogen generators use membrane technology, while Domnick Hunter uses PSA (pressure swing adsorption). Membrane units 

generally offer quieter operation, while PSA units require lower air flows. 

Nitrogen generators used for LC/MS are either fixed or variable purity units. Variable purity units have different nitrogen purities 

that are dependent on the inlet pressure, outlet pressure, and flow. Many of the variable purity units have available built-in oxygen 

analyzers to monitor the purity of the gas being provided. 


7301 

7302 

7261 

Parker Balston ® NitroFlow Lab 

Parker Balston ® N2 Series 

Domnick Hunter ® LC/MS30 

Parker Balston 

Domnick Hunter 

Model NitroFlow Lab N2-14 N2-22 N2-35 N2-45 LCMS30-1 LCMS30-0 

Flow (L/min) 30 14 22 35 45 30 30 

Air In (L/min) Built-in Compressor 73 113 171 225 Built-in Compressor 130 

Inlet Pressure (PSIG) N/A 60 60 60 60 N/A 130.5 

Outlet Pressure (PSIG) 100 145 145 145 145 102 102 

Nitrogen Purity Up to 99.5% 99% 99% 99% 99% 98% 99% 

Method Membrane Membrane Membrane Membrane Membrane PSA PSA 

Dimensions (H x W x D) 31" x 18" x 32" 51.5" x 18" x 16.2" 

67" x 24" x 20" 

20" x 28" x 32.5" 20" x 28" x 30" 

76cm x 46cm x 81cm 130.8cm x 45.7cm x 41.1cm 140cm x 61cm x 50cm 51cm x 70.5cm x 82.6cm 51cm x 70.5cm x 76cm 

Weight 285lb (130kg) 75lb (34kg) 250lb (114kg) 284lb (129kg) 298lb (135kg) 

110V Part Numbers 

without O2 analyzer 80552 80163 80293 8618464 80308 8618461 8618463 

with O2 analyzer — 80165 80297 8618466 80311 — — 

220V Part Numbers 

without O2 analyzer 80593 80169 80295 8618465 80309 8618462 8618460 

with O2 analyzer — 80171 80302 8618467 80312 — — 




UV Detectors 

Thermo UV1000 Single 

Wavelength UV Detector 

• Unique optical design reduces noise 

• Programmable wavelength changes 

• Flexibility with interchangeable flow cells 

• Comes with analytical flow cell and 

deuterium lamp 

The unique dual-beam, forward optical design 

of the Thermo UV Detector reduces noise and 

drift by focusing the light beam away from the flow call walls. 

Reference and sample beams are monitored at the same 

length for a wide, linear dynamic range. 

7424 

Thermo UV2000 Dual Wavelength UV Detector 

• Collects up to 20 spectra per run in stand alone mode 

• Dual wavelength operation for measuring absorbance 

ratios 

• Automatic lamp shut-down extends lamp life 

• Comes with analytical flow cell and tungsten and 

deuterium lamps 

Thermo’s UV2000 provides an excellent signal-to-noise 

ratio for research or routine analysis. Program different 

wavelengths to improve sensitivity or selectivity of different 

compounds. 

UV1000 Single Wavelength UV Detector Specifications 

Wavelength Range: 

190–380nm; 190–800nm with 

optional tungsten lamp 

Wavelength Accuracy: 

±1.0nm 

Wavelength Precision: 

±1.0nm 

Spectral Bandwidth: 

6nm 

Noise: 


Autosamplers 

Alltech ® 570 Autosampler 

• Affordably 

automate HPLC 

and IC analysis 

• Flushed loop 

filling maximizes 

precision and 

reproducibility 

• Partial loop filling 

minimizes sample 

waste 

Choose between flushed and partial 

loop filling and stainless steel and PEEK 

fluid paths. 

1408 

Alltech ® 580 Autosampler 

• Automates sample 

prep tasks: sample 

dilution, derivatization, 

and internal standard 

addition 

• Capable of three 

different injection 

techniques 

• Large injection volume 

range for maximum 

versatility 

• Built-in column heater 

Multifunctional autosampler 

improves laboratory efficiency. 

3000 


570 Autosampler Specifications 

Vial Capacity: 96 samples (92 with needle wash), 48 (5mL) 

vials (optional) 

Injection Volume: Flushed Loop: 5–500µL (dispenser controlled), 

5–5000µL (by head space pressure). 

Partial Loopfill: 10–490µL in steps of 10µL 

(depending on the loop volume, maximum 50% 

of the loop volume) 

Injections per Vial: 9 Maximum 

Needle Wash: Selectable, 50µL fixed volume between vials 

Reproducibility: Flushed Loop Injections: RSD≤0.5% 

Partial Loop Injections: RSD≤1.0% 

Power: 

115 VAC/230 VAC; 50/60 Hz 

Dimensions: 

11.1" H x 11.8" W x 17.3" D 

(28cm H x 30cm W x 44cm D) 

Weight: 

34lb (15.5kg) 

Warranty: 

1 year 

Model 570 Autosampler 

Description 

Part No. 

Flushed Loop Filling 

Stainless Steel 575100 

PEEK 575500 

Partial Loop Filling 


PEEK 570550 

Spare Parts for 570 Autosampler 

Description 

Part No. 

Pump Tubing Cassette 575115 

Side-Port Sample Needle, 3/pk 575130 

Large Volume Option, Field Installable 575165 

Air Needle 575125 

Sample Drain for Headspace Pressure 575170 

Auxilliary and Control I/O Cable 570101 

Tubing from Needle to Valve, 10ft 20064 

Needle Connection Nut 575175 

Needle Connection Ferrule, 10/pk 575185 

Standard Vial Tray 575110 

580 Autosampler Specifications 

Vial Capacity: 84 (1.5mL) vials + 3 (10mL) vials, 

96 (1.5mL) vials (optional), 

24 (10mL) vials (optional) 

Injection Volume: 1–5000µL in 1µL increments, 

5000–7500µL in 1.5µL increments, 

7500–10,000µL in 4µL increments 

Injections per Vial: 9 maximum 

Analysis Time: 9hrs 59min 59sec maximum 

Reproducibility: Full Loop Injections: ≤0.3% RSD, 

Partial Loop Injections: ≤0.5% RSD, 

µL Pick-Up Injections(>10µL):≤1.0% 

Carry Over: 


Column Heaters 

Grace ® and Jetstream Models 


Grace ® 7971R 

Grace ® 7990R w/Controller 

Grace ® 7956R w/Controller 

Jetstream Plus 2 

7254 

7255 7256 4298 

Specifications 

• Rugged, reliable, 

easy-to-use 

• Precise temperature 

control above ambient 

• Temperature cut-out 

with RS232 interface 

• Block heater with LED 

interface 

• Controls one or two 

columns 

• Independent 

temperatures 

• Single and dual output 

controllers 

• Long column oven 

• RS232 interface 

• Stabilizes column 

temperatures 

• Temperatures range: 

0 to 95°C 

• Ideal for GLP and 

FDA-regulated analyses 

• Heater/Chiller with long 

column oven 

• RS232 interface 

• Peltier column 

thermostat heats to 

85°C and cools to 5°C 

• Temperature may be 

fixed or changed via 

step or linear gradient 

• Large column 

compartment holds five 

columns up to 1" o.d. 

and 35cm in length 

Access to Columns Cover Cover Cover Door 

Temperature Range: 30–99°C 30–75°C 0–95°C 5–85°C 

Column Capacity 1–2, depending on size 1 column per oven 1–2, depending on size 5 

(Excluding Guards): 

Maximum Column o.d.: 1/2" 1/4" 1/2" 1" 

Maximum Column 30cm 30cm 30cm 35cm 

Length: 

Cavity Dimensions: 1" H x 2" W x 14" L 

(2.6cm H x 5.1cm W x 

0.79" H x 0.75" W x 14.2" L 

(2cm H x 1.9cm W x 36cm L) 

0.9" H x 1.7" W x 14.2" L 

(2.3cm H x 4.2cm W x 

15.4" H x 3.3" W x 2" L 

(39.2cm H x 8.5cm W x 5cm L) 

35.6cm L) 

36.1cm L) 

Temperature Accuracy: ±0.5°C ±0.5°C ±0.5°C ±0.2°C 

Temperature Stability: ±0.2°C ±0.2°C ±0.2°C ±0.1°C 

Operating Voltage: 120V/60Hz, 240V/50Hz, 250W 120V/60Hz , 240V/50Hz, 100W 120V/60Hz , 240V/50Hz, 250W 100–245VAC, 50/60 Hz, 100W 

Warranty: 1 year 1 year 1 year 1 year 

Ordering Information 

Model Grace ® 7971R Grace ® 7990R w/Controller Grace ® 7956R w/Controller Jetstream Plus 2 

Part No. Part No. Part No. Part No. 

Block heater with one 

block set and RS232 

Single output controller, 

long column oven and 

RS232 

Dual output controller, 

2 long column ovens, 

and RS232 interface 

Heater/Chiller with 

RS232 

Jetstream 2 Plus Heater/ 

Chiller, 75cm cable 

Jetstream 2 Plus Heater/ 

Chiller, 150cm cable 

5135360, 110V 

5135361, 220V 

— 

— 

— — — 

5109747, 110V 

5111660, 220V 

5109749, 110V 

5111662, 220V 

— — 

— — 

— — 

5111646, 110V 

5111645, 220V 

— — — 531102, dual voltage 

— — — 531202, dual voltage 

— 

16 



Column Heaters 

Alltech ® and Eppendorf ® Models 

Alltech ® 631 

Eppendorf ® TC-50 

Temperature Controller 

Eppendorf ® CH-30 

Eppendorf ® CH-430 and CH-460 


6219 

• Microprocessor 

controlled 

temperature 

• Column preheater 

to improve 

performance 

• Available in 

stainless steel or 

PEEK flow paths 

2336 

• Controls any 

Eppendorf Heater 

• Adjust 

temperature in 

0.1°C increments 

• Monitor “set” and 

“read” on the LED 

• Remote external 

ON or OFF feature 

2290 

• Fits one column 

up to 1/2” o.d. and 

30cm in length 

• Supports all 

column lengths 

to 30cm 

• Requires TC-50 

temperature 

controller 

2337 

• Holds four columns and two 

Rheodyne valves 

• CH-430 holds columns up to 30cm 

in length 

• CH-460 holds columns up to 50cm 

in length 

• Ideal for multi-column GPC, sample 

injection or column switching 

applications 

• Adjust temperature in 0.1°C increments 

• Requires TC-50 temperature controller 


Access To Columns Drawer Controller Cover Cover Cover 

Temperature Range: Ambient–85°C Ambient–150°C Ambient–150°C Ambient–150°C Ambient–150°C 

Column Capacity 2 NA 1 4 4 

(Excluding Guards): 

Maximum Column o.d.: 1/2" NA 1/2" 1/2" 1/2" 

Maximum Column 30cm NA 30cm 30cm 50cm 

Length: 

Cavity Dimensions: 1.8" H x 5" W x 16.5" D 

(5cm H x 13cm W x 

42cm D) 

3.25" H x 7" W x 8.5" D 

(8.3cm H x 17.8cm W x 

21.6cm D) 

3" H x 2.75" W x 19.5" D 

(7.6cm H x 7cm W x 

49.5cm D) 

3.5" H x 6.5" W x 21" D 

(8.9cm H x 16.5cm W x 

53cm D) 

3.5" H x 6.5" W x 29.5" D 

(8.9cm H x 16.5cm W x 

74.9cm D) 

Temperature Accuracy: ±1°C ±1°C ±0.5°C ±0.5°C ±0.5°C 

Temperature Stability: ±0.10°C ±0.10°C ±0.2°C ±0.2°C ±0.2°C 

Operating Voltage: 110V, 220V 110V, 220V 120V/60Hz, 240V/50Hz 

250 watts 

120V/60Hz, 240V/50Hz 

100 watts 

120V/60Hz, 240V/50Hz 

250 watts 

Inlet Preheaters: Included NA Included Four sets included 

Heater Element: Microprocessor 

controlled 

NA 

125W vulcanized to 

heating block 

300W (CH-430) or 500W 

(CH-460) vulcanized to 

heating block 

Heater Block: NA NA Aluminum, two-part oven Aluminum, two-part oven 

Sensor: NA NA 1000Ω Platinum RTD 1000Ω Platinum RTD 

Thermal Cut-Out: NA 155°C ±5°C temperature 155°C ±5°C temperature 

limit 

limit 

TC-50 Temperature No NA Yes Yes Yes 

Controller required: 

Warranty: 1 year 1 year 1 year 1 year 1 year 

CH-430 

CH-460 

Ordering Information 

Model Alltech ® 631 

Eppendorf ® TC-50 

Temperature Controller Eppendorf ® CH-30* Eppendorf ® CH-430* Eppendorf ® CH-460* 

Description Column Heater, PEEK Temperature Controllers Column Heaters Column Heaters Column Heaters 

Part No. Part No. Part No. Part No. Part No. 

110V 631100 85910 85975 85980 85971 

220V 631200 85911 85976 85981 85972 

Description 

Column Heater, SS 

110V 631300 — — — — 

220V 631400 — — — — 

*Eppendorf ® column heaters require the TC-50 controller. 




HPLC Pumps 

We Have a Pump for Every Need and Budget 

Use this pump feature comparison chart to choose the pump that is best for your application, then go to the catalog page for 

more information. 

Model Catalog Page Flow Range Isocratic/Gradient Pressure Monitoring 

301 HPLC Pump 18 0.01 - 10mL/min isocratic No 

426 HPLC Pump 18 0.01 - 10mL/min isocratic Yes 

626 HPLC Pump 19 0.001 - 12mL/min isocratic or binary gradient Yes 

627 Preparative Pump 19 0.01 - 100mL/min isocratic or binary gradient Yes 

442 Binary Gradient Pump 20 0.001 - 5.000mL/min binary gradient Yes 

801 Ultra-High Pressure Pump 20 0.001 - 5.000mL/min isocratic or binary gradient Yes 

727 Quaternary Gradient Pump 21 0.001 - 5.000mL/min quaternary gradient Yes 

Alltech ® 426 and 301 HPLC Pumps 

3 yr 

warranty 

best 

value 

6772 

Both pumps are available in stainless steel or biocompatible PEEK. 

Low-Cost, High-Performance Isocratic Pumps 

Both pumps feature: 

• Built-in pulse dampener and rapid refill for 

pulse-free operation 

• Self-flushing pump head for longer seal-life 

• Automatic prime/purge system for easy priming 

• Wide flow range for broad HPLC work, including 

semi-preparative applications 

• EZChrom driver and cables included 

The Model 426 has these additional features: 

• User-settable upper and lower pressure limits 

• Pressure monitoring on the LCD 

• Interfacing features for convenient pump control 

Model 301 HPLC Pump 426 HPLC Pump 

Flow Range: 0.01–10mL/min 0.01–10mL/min 

Isocratic/gradient: Isocratic Isocratic 

Pressure Monitoring: No Yes 

Max. Operating 

Pressure: 

6000psig (SS), 

5000psig (PEEK) 

6000psig (SS), 


Flow Precision: 0.2% RSD 0.2% RSD 

Flow Accuracy: ±2.0% ±2.0% 

Dimensions: 

6" H x 11" W x 18" D 


46cm D) 

6" H x 11" W x 18" D 


46cm D) 

Weight: 30lb (14kg) 30lb (14kg) 

Warranty: 

3-year parts, excluding 

seals, check valves, 

and pulse dampener; 

1-year labor 

3-year parts, excluding 

seals, check valves, 

and pulse dampener; 

1-year labor 

Drivers: EZChrom Elite/EZStart EZChrom Elite/EZStart 

more info 

All pumps can be controlled by EZChrom Elite or EZStart 

chromatography data systems. The driver and cables are included 

with every pump. If you wish to separately purchase the Driver 

Kit, please refer to part number 8619000. 

Model 426 and 301 HPLC Pumps 

Description 

Part No. 

Model 426 HPLC Pump 

PEEK, 85–265VAC, 47–63Hz 426150 

Stainless Steel, 85–265VAC, 47–63Hz 426250 


PEEK, 85–265VAC, 47–63Hz 301150 


Model 426 and 301 HPLC Replacement Parts 

Description 

Part No. 

Pump Head Kit, PEEK 120800 

Pump Head Kit, Stainless Steel 120799 

Piston Seal Kit 120680 

Piston 121486 

Check Valve Kit, PEEK 060141 

Check Valve Kit, Stainless Steel 120679 

Note: All replacement parts contain parts for one pump head only. 

18 



HPLC Pumps 

Alltech ® 626 HPLC Pump 

Alltech ® 627 Prep Pump 

High-Performance 

Isocratic or Binary 

Gradient Pump 

3 yr 

warranty 

6773 

Ideal for Scale-up 

Work, Semi-Prep and 

Preparative HPLC 

3 yr 

warranty 

6774 


• Suitable for standard and microflow applications 

• Dual-piston mechanism for pulse-free operation 

• Built-in low dead volume pulse dampener 

• Self-flushing pump head for longer seal life 


The Model 626 Pump is a rugged and reliable component 

for a basic analytical system, a sophisticated method 

development system, or for general laboratory or industrial 

use. It is available in either traditional 316 stainless steel 

or biocompatible PEEK. 

Buy a single 626 Pump for isocratic applications. Add a 

second 626 pump for high-pressure binary gradients. 

626 HPLC Pump Specifications 

Flow Range: 

0.001–12mL/min 

Fluid Path: 

PEEK or Stainless Steel 

Isocratic/gradient: Isocratic or binary gradient 

Pressure Monitoring: Yes 

Max. Operating Pressure: 6000psig (SS), 5000psig (PEEK) 

Flow Precision: 

0.2% RSD 

Flow Accuracy: ±2.0% 

Dimensions: 

6" H x 11" W x 18" D 


Weight: 

30lb (14kg) 

Warranty: 

3-year parts, excluding seals, check valves, 

and pulse dampener; 1-year labor 

Gradient Mixing Mode: High-Pressure 

Profiles: 

Programmable Multi-step 

Gradient Programming: 10 methods with up to 100 lines per method 

Drivers: 



Description 

Part No. 

Isocratic System 

PEEK, 85–265VAC, 47–63Hz 626150 


Binary Gradient System (Includes 2 pumps, mixing tee, 

SCU Keypad Controller, tubing, and fittings) 

PEEK, 85–265VAC, 47–63Hz 626350 


Accessories and Replacement Parts for Model 626 

Description 

Part No. 

Accessories 

SCU Keypad Controller 726300 

High-pressure Gradient Kit, PEEK 

105626 

(Includes controller, mixing tee, fittings, and tubing) 

High-pressure Gradient Kit, Stainless Steel 

105624 


Replacement Parts 


Piston 160565 


Check Valve Kit, PEEK 060141 


• Dual-piston mechanism for pulse-free operation 



• Isocratic or high-pressure binary gradient 


The Model 627 Preparative Pump delivers the accuracy and 

precision of a standard analytical pump at flow rates up to 

100mL/min. 

627 Preparative Pump Specifications 

Flow Range: 

0.01–100mL/min 

Fluid Path: 


Isocratic/gradient: Isocratic or binary gradient 


Max. Operating Pressure: 4000psig (both SS and PEEK) 


0.5% RSD 


Dimensions: 

6" H x 11" W x 18" D 


Weight: 

35lb (16kg) 

Warranty: 




Profiles: 



Drivers: 


Model 627 Preparative Pump 

Description 

Part No. 


PEEK, 85–265VAC, 47–63Hz 627150 


Binary Gradient System (Includes 2 pumps, mixing tee, 

SCU Keypad Controller, tubing, and fittings) 

PEEK, 85–265VAC, 47–63Hz 627350 


Accessories and Replacement Parts for Model 627 

Description 

Part No. 

Accessories 



105625 



105628 




Piston 121504 


Check Valve Kit, Metal-Free 060141 


related products 

Looking for preparative columns and accessories? 

See our prep section, pages 148–183. 




HPLC Pumps 

Alltech ® 442 Binary 

Gradient Pump 

3 yr 

warranty 

442 Binary Gradient Pump Specifications 

Flow Range: 

0.001–10mL/min 

Fluid Path: 


Isocratic/gradient: Binary 


Max. Operating Pressure: 6000psig (Stainless Steel) 5000psig (PEEK) 


0.5% RSD 

Flow Accuracy: 

±1% of full-scale pressure 

Dimensions: 

7" H x 10.5" W x 15.25" D 


Weight: 

24lb (11kg) 

Warranty: 



Gradient Mixing Mode: High-pressure 

Profiles: 

Programmable Multi-step through EZChrom 

Elite 

Gradient Programming: 0–100% in 0.1% increments 

Control: 

EZChrom Elite/EZStart or 

SCU Keypad Controller 

7423 

Alltech ® 801 

Ultra High-Pressure 

HPLC Pump 

3 yr 

warranty 

801 High-Pressure Pump Specifications 

Flow Range: 

0.001–5.000ml/min 

Fluid Path: 

Stainless Steel 

Isocratic/gradient: Isocratic 


Max. Operating 18,000psig 

Pressure: 

Flow Precision: 0.2% RSD 

Flow Accuracy:


HPLC Pumps 

Alltech ® 727 Quaternary Gradient HPLC Pump 

High-Pressure Quaternary Gradients for Standard 

or Micro Applications 


• Integrated pulse dampener reduces pulsations and 

mixes gradients accurately 

• Self-flushing pump heads extend seal life 

• Easy gradient programming with hand-held control 

module 

The Model 727 Quaternary Gradient Pump combines four 

high-performance pumps in a single cabinet. The system 

controller controls each pump independently, for accurate 

gradients. Flow rates are programmable from 0.001mL/min 

to 5.000mL/min making it suitable for standard or micro 

applications. The 727 pump is available in 316 stainless steel 

or biocompatible PEEK. 

new 

7425 

3 yr 

warranty 


727 Quaternary Gradient Pump Specifications 

Max. Operating Pressure: 6000psig (Stainless Steel) 


Fluid Path: 


Flow Rate Range: 0.001–5.000mL/min 


0.5% RSD 


Gradient Mixing Mode: High-pressure Gradient 

Profiles: 


Gradient Programming: 0–100% in 0.1% increments 

Dimensions: 

9" H x 15" W x 20.5" D 


Weight: 

50lb (23kg) 

Warranty: 



Control: 

EZChrom Elite/EZStart or 

SCU Keypad Controller 

Model 727 Quaternary Gradient HPLC Pump 

Description 

Part No. 

Model 727 Quaternary Gradient Pump 

PEEK, 85–265VAC, 47–63Hz 8618931 


Optional Controller 


Note: the SCU Keypad Controller is necessary to program gradients if you do not have 

EZChrom Software. 

Replacement Parts for Model 727 

Description 

Part No. 

727 Aqueous Seal Kit 5mL (for 1 head) 8619072 

727 Organic Seal Kit 5mL (for 1 head) 8619073 

Piston Assembly 5mL (for 1 head) 8619077 

PEEK Check Valve Kit (for 1 head) 8619079 

Stainless Steel Check Valve Kit (for 1 head) 8619078 



Looking for a convenient way to order 

all the essential accessories for system 

start-up? See page 121 for our HPLC 

accessory and fitting kits. 


Looking for pump accessories? 

See pages 132–135. 




Data Systems 

EZStart Chromatography Data System 

Affordable, Simple-to-use Chromatography 

Data System 

• PC-based data handling at integrator-like prices 

• Optional control of instruments from many vendors 

• Upgradeable to EZChrom Elite 

Based on the EZChrom Elite software package, EZStart is 

the ideal solution for laboratories wanting a simple-to-use 

chromatography data system with comprehensive software 

features and optional instrument control. It is intended to 

provide the basic requirements for data acquisition and 

instrument control from a single workstation. 

Operation is Reduced to Three Easy Steps: 

1. Simple configuration. Collects data from any instrument 

generating an analog output using the software’s data 

interface. 

2. Run in one window. Set up all runs from a single window 

using simple wizards. 

3. Generate graphic reports. Select from existing templates 

or customize with the report editor. 

PE Interface Control 

• Preserve your investment in existing PE Interface 

hardware 

• Take advantage of advances in computer and software 

development 

EZStart 3.1 with PE Interface Control License allows 

PerkinElmer Inc’s interface boxes to be used as analog 

to digital converters to interface chromatography detectors 

to EZStart. Control the PE Turbochrom, Turbochrom EL, 

TotalChrom ® , and ACCESS*CHROM interfaces from EZStart 

to take advantage of the software benefits while retaining the 

investment in hardware. 

PE Interface Control Software Controls the 

Following PerkinElmer ® Interfaces: 

- Series 900 Model 941/941A (RS232 communications) 

- Series 900 Models 950/950A, 960/960A, 970/970A 

(GPIB communications) 

- NCI 901/902 (GPIB and RS232 communications) 

GPIB PE Interfaces require a GPIB interface card for EZStart 

(Part No. 111420, PCI slotted card). Serial PE Interfaces 

require available RS232 ports. 

Computer Requirements: 

• Windows Vista ® “Business Edition” 

• Windows XP ® Professional (SP2) or 

2000 Professional (SP4) 

• Internet Explorer ® 6.0 or 7.0 

• Microsoft ® .NET version 2.0 

• 1.0 GHz CPU minimum 

• 512 MB RAM minimum 

• 10 GB free disk space 

• PCI slots for GPIB, SCSI 

• Multiport serial or Ethernet cards may be required 

EZStart Chromatography Data System 

Description 

Part No. 

EZStart 3.1 with PE Interface Control* 1886023 

IEEE-488 PCI Card (for PE-Nelson 700 and 900 series 111420 

intelligent interfaces)** 

*One PE interface is required but not included. 

**Available PCI slot in PC required. 

more info 

For more information on EZStart PE Interface Control Software, 

request sheet M098. Also available on our website. 

EZStart Software with SS420X Interface 

Description 

Part No. 

120V 1886011 

220V 1886211 

100V (Japan) 1886311 





7241_tech 

22 



Data Systems 

EZChrom Elite Chromatography Data System 

Premier Chromatography Data System for 

Advanced Needs 

Use EZChrom Elite software to create a fully integrated system 

to control operation, collect and store results, and record all 

settings. 

Multi-vendor instrument control: EZChrom Elite software 

works with most leading HPLC and GC instruments and 

components—over 300 vendors are currently represented, 

and more are added continuously. 

Complete data analysis: Full features and ease-of-use 

to meet your needs today and into the future. Collect 

chromatography runs and apply a variety of analysis tools 

including quantitation techniques and calibration curves. 

You can view the results graphically and generate a variety 

of outputs for reports. 

GLP/GMP features: Complete audit trails and system logs to 

document and record all changes, user security for access and 

operational privileges, and electronic signatures and sign-offs. 

EZChrom Elite Stand-Alone Package 

The EZChrom Elite Stand-Alone software package 

provides the full functionality of EZChrom Elite software for 

an individual PC. The stand-alone configurations let you 

perform all the instrument control and data processing for 

1–4 instruments. Each EZChrom Elite Stand Alone package 

contains the full functionality of EZChrom Elite software. 

Computer Requirements: 

• Windows Vista ® “Business Edition” 

• Windows XP ® Professional (SP2) or 

2000 Professional (SP4) 

• Internet Explorer ® 6.0 or 7.0 

• Microsoft ® .NET version 2.0 

• 1.0 GHz CPU minimum 

• 512 MB RAM minimum 

• 10 GB free disk space 

• PCI slots for GPIB, SCSI 

• Multiport serial or Ethernet cards may be required 


EZChrom Elite Ordering Information 

Description 

Part No. 

EZChrom Elite Stand-Alone Software* 2111696 

EZChrom Elite Stand-Alone Additional License 2111697 

EZChrom Elite Offline Re-Processing Software* 2111694 

EZChrom Elite Offline Re-Processing Additional License 2111695 

Dual-Channel A/D Interface for RS-232 8605415 

Dual-Channel A/D Interface for Ethernet/LAN 8619130 

*Licensed for a single instrument. Additional licenses can be purchased for up to 

three additional instruments. 



hplc columns | introduction 

Analytical HPLC Column 

Introduction 

Grace ® Key Column Families 

Single-Source Solution for Discovery to Recovery 

Applications 

HPLC is commonly used in a wide range of applications, 

including drug discovery and purification for the 

pharmaceutical and biotechnology industries, environmental 

analysis, forensics, petrochemical analysis, food, cosmetics, 

and vitamins. The combined premier product lines of Alltech ® , 

Davisil ® , Flexit, Grom, Jones Chromatography, 

Modcol ® , and Vydac ® create a single-source solution for 

HPLC columns and accessories from discovery to recovery. 

Our column families include reversed-phase, normal-phase, 

HILIC, ion-exchange, ion-exclusion, size-exclusion, and 

affinity stationary phases for small- and large-molecule 

separations, and our column formats maximize their 

performance. Our product range includes standard and 

custom columns for analytical separations, preparative 

phases for scale up, bulk packings that customers can 

pack in their own columns, and accessories to maintain 

separation ruggedness and quality. 

To help select the appropriate column for your application, 

we describe key column families and highlight unique phases 

within these families. Whether the most important factor 

is analysis speed, column bleed, pH stability, resolution, 

adjustable selectivity, or analyte molecular weight, Grace 

offers a column to suit your application. 

Column Families Suitable for Small Molecules 

(


Analytical HPLC Column Introduction 

Column Families Suitable for Small Molecules 

(2000 Molecular Weight) 

Alltech ® Platinum Columns 

Alternate retention and selectivity for 

polar and nonpolar analytes available in 

sub 2µm particle sizes 

Alltech ® Platinum phases have a controlled polar silica 

surface which provides enhanced polar selectivity (EPS) 

in addition to reversed-phase retention. C18 and EPS 

C18 columns are a complimentary duo to separate polar 

and nonpolar analytes. 

Differentiated Phases: C18, EPS C18 

Specifications: Spherical silica, monomerically bonded, 

proprietary endcapping, 100Å pore size 

Formats: Microbore, Expedite, Rocket, Solvent- 

Reducer, Analytical, Prep 

Vydac ® MS Columns 

Next generation Vydac ® media from the leader 

in peptide and protein separations 

Vydac ® MS columns provide unique selectivity and 

exceptional protein recovery. They require less TFA in 

the mobile phase for good peak shape, which increases 

microbore sensitivity of peptides and proteins by 

reducing “quenching”. Vydac ® MS columns are also 

applicable to hydrophobic proteins and peptide mapping. 

Differentiated Phases: C18, C8, C4 

Specifications: Spherical silica, polymerically and 

monomerically bonded, endcapped, 300Å pore size 

Formats: Capillary, Microbore, Expedite, Rocket, 

Solvent-Reducer, Analytical, Prep 


GraceSmart Columns 

Vydac ® ProZap Columns 

Classic reversed-phase and premium 

performance at an exceptional value 

GraceSmart HPLC phases use high purity silica. 

Which translates into symmetrical peaks for acids and 

bases, and predictable reversed-phase selectivity. 

Whether routine analysis or new method development, 

use individually tested GraceSmart columns to get 

premium performance at exceptional value. 

Differentiated Phases: C18 


endcapped, 120Å pore size 

Format: Analytical 

High-speed, high-efficiency columns 

for fast protein analysis 

Short 10 and 20mm ProZap columns with 1.5µm 

particles provide high-speed protein and life science 

separations. Sharp, efficient peaks maximize method 

sensitivity. 


Specifications: Sub 2µm spherical silica, proprietary 

bonding and endcapping, 500Å pore size 

Format: Expedite 

GraceAlpha Columns 

Vydac ® Everest ® Columns 

A New Silica Generation 

New silica from Grace with high porosity surface 

and dense core ideal for scale-up applications. 

While high porosity surface increases mass transfer 

results in increased column efficiencies and loading 

capacity, dense core and highly spherical shape yields 

mechanically robust particle. 

Differentiated Phases: C18, C8, and Silica 

Specifications: High purity spherical silica with high 

porosity surface and dense core 

Formats: Analytical, Prep 

For peptide separations, peptide 

mapping and proteomics applications 

High-capacity Everest ® C18 columns are recommended 

as the first choice in peptide separations. Everest ® 

offer high capacity to resolve complex samples such as 

protein digests. 



endcapped, 300Å pore size 

Formats: Capillary, Microbore, Expedite, Analytical 




HPLC Column Selection 

A Comparison of Reversed-Phase Columns 

Based on the widely accepted work and data of Drs. Lloyd Snyder and John Dolan. 1,2 Grace has developed this column selection 

tool for choosing reversed phase HPLC columns based on peak capacities and column selectivity of polar and nonpolar compounds. 

Typically, chromatographers choose HPLC columns by comparing physical characteristics, such as surface area and carbon load. 

Often, this does not provide enough information about selectivity or capacity for adequate column selection. This chart provides a 

reliable means of choosing HPLC columns based on acidic, basic, and hydrophobic character. 

The Snyder/Dolan column test procedure has been described in a series of publications. Based on retention data for a series of 

standard mixtures and the same separation conditions (50% acetonitrile/buffer; pH 2.8 and 7.0; 35°C), reversed-phase columns 

are characterized by five column-selectivity parameters: hydrophobicity (H), steric interaction (S*), hydrogen-bond acidity A, 

basicity (B), and relative silanol ionization or cation-exchange capacity C. Here we have chosen to graphically highlight data 

for H , A, and C, with C results at pH 7.0. Hydrophobicity (H) is often the primary analyte interaction with reversed phase 

columns and indicates overall capacity. Secondary interactions are often polar interactions with basic analytes. The degree of 

unprotonated base interaction A and protonated base interaction C with the packing material is measured and represented here. 

Directions for Using the Column Chart 

The chart lists the columns in descending order of hydrophobic capacity (H) . To find similar HPLC columns to test as 

back-up columns, follow these steps. 

1) Find the column you are currently using and note neighboring columns which have similar (H) capacity factors. 

2) Compare the values for interaction of polar compounds ( A and C ). 

If there is more than one choice for a back-up column, then compare your actual sample to the test probes. If your 

sample is nonpolar, then place more emphasis on hydrophobic values. If your sample is basic (polar), then pay special 

attention to A and C and determine if your sample will be protonated ( A) or unprotonated (C ) and place greater 

emphasis on one of these values. 

Key to Chart 

Hydrophobic Indicator 

A Hydrogen bonding 

Indicator—pH 2.8 

(Protonated under 

acidic conditions) 

C Cation Exchange 

Indicator—pH 7.0 

(Unprotonated under 

neutral conditions) 

Manufacturer Column Selectivity Parameters 

Manufacturer Column Selectivity Parameters 

ZirChrom ZirChrom ® -PBD C18 A C 1.284 Merck LiChrospher ® 100 RP-18 C A 

1.006 

YMC J’Sphere ® H80 C18 C A 

1.132 

Bischoff ProntoSIL 120-5-C18 H C A 

1.005 

Restek Allure ® C18 C A 

1.116 

Shiseido CAPCELL C18 M G C A 

1.005 

Phenomenex Ultracarb ® ODS (30) C A 

1.114 

Grace (Jones) Genesis ® 120 C18 C A 

1.005 

YMC YMC ® Pack Pro C18 RS C A 

1.114 

Bischoff EU Reference Column C18 C A 

1.004 

Grace (Alltech) Adsorbosphere UHS C18 C 

A 

1.103 

Grace (Alltech) Allsphere ODS2 CA 

1.004 

Thermo/Hypersil Hypersil ® BetamaxNeutral C18 C A 

1.098 

Merck Purospher ® STAR RP18e C A 

1.003 

Agilent Zorbax Extend C18 C A 

1.098 

Merck Chromolith ® RP18e C A 

1.003 

Agilent Zorbax C18 C A 

1.089 

Phenomenex Luna ® C18(2) C A 

1.002 

Beckman Ultrasphere ® ODS C A 

1.085 

Varian Pursuit ® C18 C A 

1.001 

Grace (Alltech) Alltima HP C18 High Load C A 

1.080 

MacMod/ACT ACE ® 5 C18 C A 

1.000 

Agilent Zorbax Rx-18 C A 

1.077 

Tosoh TSKgel ® Super-ODS C A 

0.998 

Agilent Zorbax Eclipse XDB-C18 C A 

1.077 

Agilent Zorbax StableBond 80A C18 C A 

0.996 

Supelco Ascentis ® C18 C A 

1.077 

Phenomenex Prodigy ODS(2) C A 

0.995 

Macherey Nagel Nucleodur ® C18 Gravity C A 

1.056 

Thermo/Hypersil Hypersil ® BDS C18 C A 

0.993 

Grace (Grom) Grom Sapphire 110 C18 C A 

1.055 

Grace (Alltech) Alltima C18 C A 

0.993 

Restek Restek ® Ultra C18 C A 

1.055 

Grace (Vydac) Vydac ® Everest ® C18 C A 

0.993 

Varian OmniSpher 5 C18 C A 

1.055 

Thermo/Hypersil Hypersil ® Beta Basic-18 C A 

0.993 

Grace (Vydac) Denali ® 120 C18 C A 

1.052 

GL Sciences Inertsil ® ODS-3 C A 

0.990 

Waters Symmetry ® C18 C A 

1.052 

Grace (Alltech) Adsorbosphere C18 A C 

0.989 

Akzo Nobel Kromasil ® 100-5C18 C A 

1.051 

Phenomenex Synergi Max-RP C18 C A 

0.989 

Waters Nova-Pak ® C18 C A 

1.049 

Shiseido CAPCELL C18 SG120 C A 

0.987 

Thermo/Hypersil Hypersil ® 100 C18 C A 

1.048 

Grace (Jones) Apex I C18 A C 

0.985 

MacMod/ACT ACE ® 5 C18-HL C A 

1.045 

Thermo/Hypersil Hypersil ® ODS-2 C A 

0.985 

ZirChrom ZirChrom ® -EZ C18 A 

C 

1.040 

Grace (Alltech) Alltima HP C18 C A 

0.985 

Grace (Grom) Grom Sil 120 ODS-5 ST C A 

1.035 

Waters Xterra ® MS C18 C A 

0.984 

Dionex Acclaim ® 120 C18 C A 

1.032 

Waters Symmetry ® 300 C18 C A 

0.984 

Waters Sunfire C18 C A 

1.031 

Supelco Discovery C18 C A 

0.984 

Agilent Zorbax Eclipse Plus C18 C A 

1.030 

Supelco Supelcosil LC-18-DB C A 

0.979 

Merck Superspher ® 100 RP-18e C A 

1.030 

Waters Spherisorb ® S5 ODSB A C 

0.975 

Shiseido CAPCELL C18 AG120 C A 

1.030 

Thermo/Hypersil Hypersil ® Bio Basic-18 C A 

0.974 

Grace (Grom) Grom Sil 120 ODS-3 CP C A 

1.029 

Thermo/Hypersil Hypersil ® ODS A C 

0.974 

Waters Delta-Pak C18 100A C A 

1.028 

Bischoff ProntoSIL 120-5-C18-AQ C A 

0.974 

Macherey Nagel Nucleodur ® Isis C A 

1.023 

Grace (Jones) Genesis ® 300 C18 C18 C A 

0.974 

Phenomenex Prodigy ODS (3) C A 

1.023 

Phenomenex Synergi Hydro-RP C18 C A 

1.022 

Phenomenex Luna C18 C A 

1.018 

Supelco Supelcosil LC-18 A C 

1.018 

YMC YMC ® Pro C18 C A 

1.015 

Phenomenex Onyx Monolithic C18 C A 

1.012 

Bischoff ProntoSIL SpheriBOND 80-5-ODS2 C A 

1.010 

Grace (Jones) Apex II C18 A C 

1.008 

Shiseido CAPCELL C18 UG120 C A 

1.007 

GL Sciences Inertsil ® ODS-2 C A 

1.007 

Bischoff Prontosil 200-5-C18 AQ C A 

0.974 

Agilent Zorbax C8 C A 

0.974 

Tosoh TSK gel ® ODS-80Ts A C 

0.971 

Waters Resolve C18 A C 

0.968 

Phenomenex Gemini ® C18 110A C A 

0.967 

Grace (Alltech) Econosil C18 C A 

0.966 

Phenomenex Aqua ® C18 C A 

0.966 

YMC YMC ® ODS-AQ C18 C A 

0.965 

Waters Spherisorb ® ODS-2 AC 

0.962 

Macherey Nagel Nucleosil ® 100-5-C18 HD C18 C A 

0.961 

26 



Grace (Jones) Genesis ® 120 AQ C18 C A 

0.960 

Macherey Nagel Nucleodur ® Pyramid C A 

0.958 

Thermo/Hypersil Hypersil ® Elite C18 C A 

0.958 

Dionex Acclaim ® 300 C18 C A 

0.957 

Bischoff ProntoSIL 300-5-C18 H C A 

0.956 

Waters Delta-Pak C18 300A C A 

0.955 

Bischoff ProntoSIL HyperSORB 120 ODS A C 

0.951 

Thermo/Hypersil Hypersil ® PAH C18 A C 

0.949 

Bischoff ProntoSIL 120-5-C18 Aqplus C A 

0.947 

Phenomenex Jupiter ® 300 C18 C A 

0.945 

Varian Polaris ® C18-Ether 0.943 

CA 

Waters Atlantis ® T3 C18 AC 

0.941 

Tosoh TSKgel ® 80Ts QA C A 

0.940 

Grace (Alltech) Alltima C18-WP C A 

0.938 

Waters YMC ® Hydrosphere C18 C A 

0.937 

Grace (Alltech) Brava BDS C18 C A 

0.938 

Bischoff ProntoSIL 60-5 C8 SH 0.929 

C A 

Varian Polaris ® C18-A C A 

0.928 

YMC J’Sphere ® M80 C18 C A 

0.926 

Agilent Zorbax Eclipse XDB-C8 C A 

0.919 

Waters Atlantis ® dC18 b C A 

0.917 

Supelco Ascentis ® Express C8 C A 

0.915 

Thermo Hypersil ® GOLD aQ 0.915 

C A 

Phenomenex Selectosil C18 C A 

0.911 

Merck LiChrosorb ® RP-18 C A 

0.909 

Grace (Vydac) Vydac ® 218TP C18 C A 

0.909 

Waters Acquity UPLC ® BEH Shield RP18 EP C A 

0.907 

Macherey Nagel Nucleosil ® C18 0.906 

C A 

Agilent Zorbax StableBond 300A C18 C A 

0.905 

Grace (Alltech) Prosphere C18 300 AC 

0.903 

Grace (Vydac) Vydac ® 201TP C18 C A 

0.901 

Supelco Ascentis ® C-8 C A 

0.899 

Waters Nova-Pak ® C8 0.899 

CA 

Waters Symmetry ® C8 C A 

0.893 

YMC YMC ® Pro C8 C A 

0.890 

Agilent Zorbax Eclipse Plus C8 C A 

0.889 

Phenomenex Luna C8(2) C A 

0.889 

Grace (Alltech) Prevail C18 C A 

0.888 

Grace (Alltech) Prosphere 100 C18 AC 

0.883 

Grace (Alltech) Alltima AQ EP C A 

0.882 

Thermo/Hypersil Hypersil ® GOLD C18 C A 

0.881 

Phenomenex Synergi Fusion-RP EP C A 

0.879 


0.875 

Grace (Grom) Grom Sil 120 Octyl-6 MB C8 C A 

0.872 

Grace (Jones) Apex I C8 0.869 

Shiseido CAPCELL C18 A Q C A 

0.867 

Macherey Nagel Nucelosil ® 100-5-C8 HD C A 

0.865 

Akzo Nobel Kromasil ® 100-5C8 C A 

0.864 

Grace (Jones) Genesis ® 120 EC C8 C A 

0.863 

Grace (Alltech) Prevail Amide EP C A 

0.862 

Macherey Nagel Nucleosil ® ODS AC 

0.860 

Restek Ultra AQ C18 C A 

0.857 

Waters Sunfire C8 C A 

0.856 

Waters Acquity UPLC ® BEH C8 C A 

0.855 

Shiseido CAPCELL PAK C8 UG120 C A 

0.854 

Waters Symmetry ® Shield C18 AC 

0.850 

Grace (Alltech) Alphabond C18 A C 

0.845 

Supelco Ascentis ® RP-Amide AC 

0.843 

Merck Purospher ® RP-18 C A 

0.841 

Supelco Discovery BIO Wide pore C8 C A 

0.839 

Grace (Grom) Grom Sapphire 110 C8 C A 

0.835 

Thermo/Hypersil Hypersil ® Beta Basic-8 C A 

0.834 

Grace (Alltech) Alltima HP C8 C A 

0.834 

Thermo/Hypersil Hypurity ® C8 C A 

0.833 

Supelco Discovery C8 C A 

0.832 

GL Sciences Inertsil ® C8-3 C8 C A 

0.830 

MacMod/ACT ACE ® 5 C8 C A 

0.830 

Grace (Jones) Genesis ® 120 C8 C A 

0.829 

Thermo Hypersil ® GOLD C8 C A 

0.825 

Phenomenex Onyx Monolithic C8 C A 

0.824 

Tosoh TSKgel ® Super-Octyl C A 

0.824 

Grace (Alltech) Prevail Select C18 AC 

0.822 

Thermo/Hypersil Hypersil ® Bio Basic-8 C 

0.821 

YMC YMC ® Basic C18 C A 

0.821 

Grace (Alltech) Econosphere C18 A C 

0.818 

Tosoh TSKgel ® Octyl-80Ts AC 

0.814 

Whatman Partisil ODS(3) AC 

0.810 

A 

AC 

Macherey Nagel Nucleodur ® Sphinx RP AC 

0.805 

Thermo/Hypersil Aquasil C18 C A 

0.805 

MacMod/ACT ACE ® AQ EP C A 

0.804 

Waters Xterra ® MS C8 C A 

0.803 


0.800 

Waters MicroBondapak C18 AC 

0.798 

Agilent Zorbax StableBond 80A C8 0.795 

C A 

Agilent Zorbax Rx-C8 0.792 

C A 

Grace (Alltech) Platinum C18 AC 

0.786 

Grace VisionHT C18 AC 

0.786 

Phenomenex Luna Phenyl-Hexyl AC 

0.782 

Grace (Alltech) Alltima C18-LL C A 

0.780 

Bischoff ProntoSIL 120-5-C18 ace-EPS A C 

0.772 

Grace (Vydac) Vydac ® 218MS C18 AC 

0.770 

Waters Acquity UPLC ® BEH phenyl 0.764 

C A 

Waters Spherisorb ® C8 AC 

0.763 

YMC J’Sphere ® L80 C18 0.762 

C A 

Bischoff ProntoSIL 300-55-C18 ace-EPS A C 

0.762 

Dionex Acclaim ® Organic Acid C18 AC 

0.761 

Waters Xterra ® C18 RP 0.757 

C A 

Grace (Alltech) Alltima C8 0.756 

C A 

Whatman Partisil C8 0.749 

C A 

Merck LiChrospher ® 60 RP-Select B C18 AC 

0.747 

Bischoff ProntoSIL 120-5 C8 SH C A 

0.739 

Grace (Alltech) Allsphere ODS1 C A 

0.733 

Waters Symmetry ® Shield C8 A C 

0.730 

Thermo/Hypersil Hypurity ® C4 0.713 

CA 

MacMod/ACT ACE ® 5 C4-300 C A 

0.710 

Varian Polaris ® C8-Ether AC 

0.705 

Bischoff ProntoSIL 60-5-Phenyl A C 

0.705 

Waters Nova-Pak ® Phenyl A C 

0.704 

Macherey Nagel Nucleosil ® 100-5-C18 Nautilus A C 

0.702 


CA 

Bischoff ProntoSIL SpheriBOND 80-5-ODS1 A C 0.700 

Thermo/Hypersil Fluophase ® RP F AC 

0.698 

Phenomenex Jupiter ® 300 C4 AC 

0.698 

Grace (Alltech) Prosphere 300 C4 0.689 

CA 

Bischoff Prontosil 60-5-C4 AC 

0.686 

Waters Xterra ® Phenyl 0.683 

C A 

Waters Spherisorb ® ODS-1 C A 

0.682 

Thermo/Hypersil Hypersil ® Prism C18 RPN 0.678 

C A 

Thermo/Hypersil Fluophase ® PFP F A C 

0.675 

Agilent Zorbax XDB-Phenyl AC 

0.665 

Waters Symmetry ® 300 C4 AC 

0.659 

Waters Xterra ® C8 RP AC 

0.657 

Grace (Alltech) Alltima HP C18 EPS C A 0.655 

Supelco Discovery BIO Wide pore C5 0.654 

CA 

Agilent Zorbax Bonus RP EP A C 

0.654 

Phenomenex Synergi Polar-RP C18 AC 

0.654 

MacMod/ACT ACE ® Phenyl AC 

0.647 

Grace (Jones) Genesis ® 120 C4 EC AC 

0.646 

Thermo/Hypersil Hypersil ® Prism C18 RP C A 

0.645 

Thermo/Hypersil BetaMax ® Acid EP 0.635 

C A 

Supelco Discovery HS F5 F A C 

0.631 

Agilent Zorbax SB-Phenyl AC 

0.623 

Grace (Alltech) Platinum EPS C18 AC 0.619 

Grace VisionHT C18-P AC 0.619 

Grace (Alltech) Prevail C8 AC 

0.617 

Grace (Jones) Genesis ® 300 C4 C4 AC 

0.615 

Grace (Jones) Genesis ® Phenyl A C 

0.609 

Agilent Zorbax StableBond 80A C3 AC 

0.601 

MacMod/ACT ACE ® Phenyl-300 AC 

0.599 

Agilent Zorbax SB-AQ EP AC 

0.593 

ZirChrom ZirChrom ® -PS EP A 

C 0.589 

Waters MicroBondapak Phenyl A C 

0.585 

Grace (Alltech) Platinum C8 0.584 

CA 

Grace (Alltech) Platinum EPS C8 300 A C 

0.584 

Thermo/Hypersil BetaBasic ® Phenyl A C 

0.582 

Macherey Nagel Nucleosil ® C8 AC 0.575 

Macherey Nagel EC Nucleosil ® 100-5 Protect 1 EP C A 

0.544 

Bischoff Prontosil 120-5-C8 ace-EPS A C 

0.532 

Phenomenex Prodigy Phenyl-3 A C 0.529 


C A 

Grace (Alltech) Alltima HP C18 Amide C A 0.497 

Thermo/Hypersil BetaMax ® Base EP A C 0.470 

Grace (Alltech) Platinum EPS C8 AC 0.420 

Thermo/Hypersil Hypurity ® Advance AC 0.412 

References: 

1. “The “Hydrophobic-subtraction” Model of Reversed-phase Column Selectivity”, L.R. Snyder, J.W. Dolan and P.W. Carr, J. Chromatogr. A, 1060 (2004) 77-116. 

2. “A New Look at the Selectivity of Reversed-phase HPLC Columns”, L.R. Snyder, J.W. Dolan and P.W. Carr, Anal. Chem., 79 (2007) 3255-3262. 





28 

Grace ® HPLC Packing Material Specifications 

Columns for Small Molecules 

Base 

Material 

Particle 

Shape Particle Size Pore Size Surface Area 

Carbon 

Load 

Endcapped? 

USP 

L-code 

Brand 

Phase 

Phase Type 

Adsorbosil ® C18 Silica Irregular 5, 10µm 60Å 450m 2 /g 15% Polymeric Yes L1 

C8 Silica Irregular 5, 10µm 60Å 450m 2 /g 10% Polymeric Yes L7 

C2 Silica Irregular 5, 10µm 60Å 450m 2 /g No Polymeric No L16 

CN Silica Irregular 5, 10µm 60Å 450m 2 /g — Polymeric Yes L10 

NH2 Silica Irregular 5, 10µm 60Å 450m 2 /g — Polymeric No L8 

Silica Silica Irregular 5, 10µm 60Å 450m 2 /g — Polymeric No L3 

Adsorbosphere C18 Silica Spherical 3, 5, 10µm 80Å 200m 2 /g 12% Monomeric Yes L1 

C18 HS Silica Spherical 3, 5µm 60Å 350m 2 /g 20% Monomeric Yes L1 

C18 UHS Silica Spherical 5, 10µm 60Å 500m 2 /g 30% Monomeric Yes L1 

C8 Silica Spherical 3, 5, 10µm 80Å 200m 2 /g 8% Monomeric Yes L7 

Phenyl Silica Spherical 5µm 80Å 200m 2 /g 5% Monomeric Yes L11 

Cyano Silica Spherical 5µm 80Å 200m 2 /g — Monomeric Yes L10 

Cyano-AQ Silica Spherical 5µm 120Å 170m 2 /g — Polymeric No L10 

Amino (NH2) Silica Spherical 3, 5µm 80Å 200m 2 /g — Polymeric No L8 

Silica Silica Spherical 5µm 80Å 200m 2 /g — — No L3 

SAX Silica Spherical 5µm 80Å 200m 2 /g — Monomeric No — 

SCX Silica Spherical 5µm 80Å 200m 2 /g — Monomeric Yes — 

Adsorbosphere XL C18 Silica Spherical 3, 5µm 90Å 200m 2 /g 11% Monomeric Yes L1 

C18-B Silica Spherical 5µm 90Å 200m 2 /g 12% Monomeric Yes L1 

C8 Silica Spherical 3, 5µm 90Å 200m 2 /g 6% Monomeric Yes L7 

C1 (TMS) Silica Spherical 5µm 90Å 200m 2 /g — Monomeric Yes L13 


SAX Silica Spherical 5, 10µm 90Å 200m 2 /g — Monomeric Yes — 

SCX Silica Spherical 5, 10µm 90Å 200m 2 /g — Monomeric Yes — 

Allsphere ODS-1 Silica Spherical 5µm 80Å 220m 2 /g 7% Monomeric Partial L1 

ODS-2 Silica Spherical 3, 5µm 80Å 220m 2 /g 12% Monomeric Yes L1 


C6 Silica Spherical 5µm 80Å 220m 2 /g 4% Monomeric Yes L15 

C1 (TMS) Silica Spherical 5µm 80Å 220m 2 /g 3% Monomeric No L13 


Cyano Silica Spherical 5µm 80Å 220m 2 /g 3.5% Monomeric No L10 

Amino (NH2) Silica Spherical 5µm 80Å 220m 2 /g 3% Monomeric No L8 

Silica Silica Spherical 3, 5µm 80Å 220m 2 /g — — No L3 

SAX Silica Spherical 5µm 100Å 220m 2 /g 4% Monomeric No — 

SCX Silica Spherical 5µm 100Å 220m 2 /g 4% Monomeric No — 

Alltima HP C18 Silica Spherical 3, 5µm 190Å 200m 2 /g 12% Monomeric Yes L1 

C18 EPS Silica Spherical 3, 5µm 190Å 200m 2 /g 4% Monomeric No L1 

C18 HiLoad Silica Spherical 3, 5µm 100Å 450m 2 /g 24% Monomeric Yes L1 

C18 AQ Silica Spherical 3, 5µm 100Å 450m 2 /g 20% Monomeric Yes L1 

C18 Amide Silica Spherical 3, 5µm 190Å 200m 2 /g 12% Monomeric Yes L1 


Cyano Silica Spherical 3, 5µm 190Å 200m 2 /g 4% Monomeric Yes L10 


HILIC Silica Spherical 1.5, 3, 5µm 120Å 230m 2 /g — — No L3 

Alltima C18 Silica Spherical 3, 5, 10µm 100Å 340m 2 /g 16% Polymeric Yes L1 

C18 LL Silica Spherical 5µm 100Å 340m 2 /g 9% Polymeric Yes L1 

C8 Silica Spherical 3, 5, 10µm 100Å 340m 2 /g 9% Polymeric Yes L7 

Phenyl Silica Spherical 3, 5µm 100Å 340m 2 /g 7.5% Polymeric Yes L11 

Cyano Silica Spherical 3, 5µm 100Å 340m 2 /g — Polymeric Yes L10 


Silica Silica Spherical 3, 5, 10µm 100Å 340m 2 /g — — No L3 

AlphaBond C18 Silica Irregular 5, 10µm 125Å 300m 2 /g 10% Monomeric Yes L1 

C8 Silica Irregular 10µm 125Å 300m 2 /g — Monomeric Yes L7 

Phenyl Silica Irregular 10µm 125Å 300m 2 /g — Monomeric Yes L11 

Cyano Silica Irregular 10µm 125Å 300m 2 /g — Monomeric Yes L10 

Amino (NH2) Silica Irregular 10µm 125Å 300m 2 /g — Polymeric No L8 

Silica Silica Irregular 10µm 125Å 300m 2 /g — — No L3 

Apex I ODS Silica Spherical 3, 5, 10µm 100Å 170m 2 /g 10% Polymeric Yes L1 

C8 Silica Spherical 3, 5µm 100Å 170m 2 /g 7% Monomeric No L7 

C8(EC) Silica Spherical 3, 5µm 100Å 170m 2 /g 7% Monomeric Yes L7 

C1 Silica Spherical 3, 5µm 100Å 170m 2 /g 2.5% Monomeric Yes L13 

Phe Silica Spherical 3, 5µm 100Å 170m 2 /g 3% Monomeric No L11 

Basic ODS Silica Spherical 5µm 100Å 200m 2 /g 12% Monomeric Yes L1 

PAH Silica Spherical 5µm 100Å 170m 2 /g — Monomeric Yes 

CN Silica Spherical 3, 5µm 100Å 170m 2 /g 4% Monomeric No L10 

Amino (NH2) Silica Spherical 3, 5µm 100Å 170m 2 /g 2% Monomeric No L8 

Carbohydrate Silica Spherical 5µm 100Å 170m 2 /g — Monomeric † 


Apex II ODS Silica Spherical 3, 5µm 100Å 170m 2 /g 10.5% Monomeric Yes L1 

Diol Silica Spherical 3, 5µm 100Å 170m 2 /g 3.5% Monomeric No 


Others—as Apex I Silica Spherical 5µm 100Å 170m 2 /g — — No — 

Apex Prepsil ODS Silica Spherical 8, 15µm 130Å 170m 2 /g 10% Polymeric Yes L1 



C2 Silica Spherical 8µm 130Å 170m 2 /g 2.8% Monomeric No L30 

CN Silica Spherical 8µm 130Å 170m 2 /g 4% Monomeric Yes L10 

Amino (NH2) Silica Spherical 8µm 130Å 170m 2 /g 2% Monomeric Yes L8 


Diol Silica Spherical 8µm 130Å 170m 2 /g 3.2% Monomeric No L20 

†Proprietary. 



Columns for Small Molecules (continued) 

Base 

Material 

Particle 


Carbon 

Load 

Endcapped? 

USP 

L-code 

Brand 

Phase 

Phase Type 

Apollo C18 Silica Spherical 5µm 100Å 340m 2 /g 15% Monomeric Yes L1 




Brava C18 BDS Silica Spherical 3, 5µm 145Å 185m 2 /g 8.5% Monomeric Yes L1 

C18 ODS Silica Spherical 3, 5µm 130Å 195m 2 /g 8.5% Monomeric Yes L1 


C8 BDS Silica Spherical 3, 5µm 145Å 185m 2 /g 5.5% Monomeric Yes L7 

Phenyl Silica Spherical 5µm 130Å 195m 2 /g — Monomeric No L11 

Cyano Silica Spherical 5µm 130Å 195m 2 /g — Monomeric No L10 

Cyano BDS Silica Spherical 5µm 145Å 185m 2 /g — Monomeric No L10 

Amino (NH2) Silica Spherical 5µm 130Å 195m 2 /g — Monomeric No L8 


Carbohydrate Amino Silica Irregular 10µm 80Å 550m 2 /g — Polymeric No — 

Cation Polymer Spherical 10µm — — — — No — 

Denali ® 238DE C18 Silica Spherical 3, 5, 10, 15, 20µm 120Å 280-340m 2 /g 20% Monomeric Yes L1 

Econosil* C18 Silica Irregular 3, 5, 10µm 80Å 200m 2 /g 10% Monomeric Yes L1 

C8 Silica Irregular 3, 5, 10µm 80Å 200m 2 /g 5% Monomeric Yes L7 

CN Silica Irregular 5, 10µm 80Å 200m 2 /g — Monomeric Yes L10 

NH2 Silica Irregular 5, 10µm 80Å 200m 2 /g — Polymeric No L8 

Silica Silica Irregular 3, 5, 10µm 80Å 200m 2 /g — — No L3 

Econosphere* C18 Silica Spherical 3, 5, 10µm 80Å 200m 2 /g 10% Monomeric Yes L1 



Amino (NH2) Silica Spherical 5µm 80Å 200m 2 /g — Polymeric No L8 


Genesis ® 120 C18 Silica Spherical 3, 4, 7, 15µm 120Å 300m 2 /g 18% Monomeric Yes L1 

C18 AQ Silica Spherical 4, 7µm 120Å 300m 2 /g 15% Monomeric Yes L1 

C8 Silica Spherical 3, 4, 7, 15µm 120Å 300m 2 /g 11% Monomeric No L7 

C8(EC) Silica Spherical 3, 4, 7, 15µm 120Å 300m 2 /g 11% Monomeric Yes L7 



CN Silica Spherical 3, 4µm 120Å 300m 2 /g 7% Monomeric Yes L10 

Amino (NH2) Silica Spherical 3, 4µm 120Å 300m 2 /g 3.5% Polymeric No L8 

Carbohydrate Silica Spherical 4µm 120Å 300m 2 /g — Monomeric No — 

CN-TCA Silica Spherical 4µm 120Å 300m 2 /g 7% Monomeric Yes — 

Petro-XP Silica Spherical 4µm 120Å 300m 2 /g — Monomeric No — 

Silica Silica Spherical 3, 4, 7, 15µm 120Å 300m 2 /g — — No L3 

Grace Alpha ® C18 Silica Spherical 5, 10, 15, 20µm 120Å 325m 2 /g 15% Monomeric Yes L1 


Silica Silica Spherical 5, 10, 15, 20µm 120Å 325m 2 /g — — No L3 

GraceSmart C18 Silica Spherical 3, 5µm 120Å 220m 2 /g 10% Monomeric Yes L1 

Grom Sil ODS-0 AB (acid/base deactivated) Silica Spherical 1.5, 3, 5, 10µm 100Å 200m 2 /g 11% Monomeric Yes L1 

ODS-2 FE (fully endcapped) Silica Spherical 1.5, 3, 5, 10µm 80, 100, 300Å 220, 200, 100m 2 /g 12, 11, 6% Monomeric Yes L1 

ODS-3 CP (encapsulated) Silica Spherical 3, 5, 7, 10µm 120, 300Å 320, 170m 2 /g 15, 6% Polymeric No L1 

ODS-4 HE (hydrophilic endcapping) Silica Spherical 3, 4, 5, 7, 10µm 120, 200Å 300, 200m 2 /g 16, 11% Monomeric Yes L1 

ODS-5 ST (standard) Silica Spherical 3, 4, 5, 7, 10µm 60, 120, 200, 300Å 580, 300, 200, 22, 17, Monomeric Yes L1 

150m 2 /g 

12, 7% 

ODS-6 NE (non endcapped) Silica Irregular 3, 5µm 120Å 300m 2 /g 17% Monomeric No L1 

ODS-7 pH (pH-stable) Silica Irregular 4µm 80Å 510m 2 /g 22% Polymeric No L1 

Octyl-1 B (base deactivated) Silica Spherical 3, 5µm 100Å 200m 2 /g 6.5% Monomeric Yes L7 

Octyl-2 AB (acid/base deactivated) Silica Spherical 3, 5µm 100Å 200m 2 /g 5% Monomeric Yes L7 

Octyl-3 BA (for bases) Silica Spherical 3, 5µm 120Å 300m 2 /g 9% Monomeric Yes L7 

Octyl-4 FE (fully endcapped) Silica Spherical 3, 5, 10µm 80, 100, 300Å 220, 200, 100m 2 /g 6.6, 6, 3% Monomeric Yes L7 

Octyl-5 CP (encapsulated) Silica Spherical 3, 5, 7, 10µm 120, 300Å 320, 170m 2 /g 10, 5.5% Polymeric No L7 

Octyl-6 MB (monomer binding) Silica Spherical 3, 5, 10µm 120, 200, 300Å 300, 200, 150m 2 /g 10, 7, 4% Monomeric Yes L7 

Hexyl-1 MB (monomeric bonding) Silica Spherical 5µm 80, 100Å 220, 200m 2 /g 4, 4% Monomeric Yes 

Phenyl-1 FE (fully endcapped) Silica Spherical 3, 5, 10µm 120, 300Å 300, 150m 2 /g 9, 5% Monomeric Yes L11 

Phenyl-2 CP (encapsulated) Silica Irregular 5µm 120, 300Å 320, 170m 2 /g 7, 4% Polymeric No L11 

Phenyl-3 PE (partially endcapped) Silica Spherical 3, 5, 10µm 80, 100Å 220, 200m 2 /g 6.6, 6% Monomeric Yes L11 

Butyl-1 ST (standard) Silica Spherical 3, 5µm 120, 300Å 300, 150m 2 /g 7, 2.5% Monomeric No L26 

Butyl-2 FE (fully endcapped) Silica Spherical 3, 5µm 300Å 100m 2 /g 1, 5% Monomeric No L26 

TMS-1 ST (standard) Silica Spherical 3, 5µm 120, 300Å 300, 150m 2 /g 4% Monomeric Yes L13 

TMS-2 CP (encapsulated) Silica Spherical 3, 5µm 120, 300Å 320, 170m 2 /g 3% Polymeric No L13 

Cyan-1 ST (standard) Silica Spherical 3, 5µm 120, 300Å 300, 150m 2 /g 4.8% Monomeric Yes 

Cyan-2 PR (cyanopropyl) Silica Spherical 3, 5µm 80, 100Å 220, 200m 2 /g 3.5% Monomeric Yes 

Cyan-3 CP (encapsulated) Silica Spherical 5µm 120Å 320m 2 /g 4% Polymeric No 

Amino-1 PR (NH-propyl) Silica Spherical 3, 5, 10µm 80, 100Å 220, 200m 2 /g 2% Monomeric Yes L8 

Amino-2 PA (cross linked Poly-NH2) Silica Spherical 5µm 120Å 300m 2 /g — Polymeric No L8 

Amino-3 CP (encapsulated NH-residues) Silica Irregular 5µm 80Å 420m 2 /g — Monomeric Yes L8 

Amino-4 PR (propylamine bonded Silica Irregular 3, 7µm 300Å 100m 2 /g — Monomeric No L8 

to silica) 

Diol Silica Spherical 5, 10µm 60, 120, 200, 300Å 580, 300, 200, — Monomeric No L20 

150m 2 /g 

Normal Phase-1 ST (standard silica) Silica Spherical 3, 5, 10µm 80, 100, 1000Å 220, 200m 2 /g — — No L3 

Normal Phase-2 SP (spherical silica) Silica Spherical 3, 5, 10µm 60, 120, 200, 580, 300, 200m 2 /g — — No L3 

1000Å 

Normal Phase-3 PV (polyvinylalcohol) Silica Spherical 5µm 120Å 300m 2 /g — Polymeric No L3 

SEC (size exclusion chromatography) Silica Spherical 5, 10µm 60, 120, 200, 300Å 580, 300, 200, — — No 

150m 2 /g 

Strong Anion-1 Silica Spherical 5, 10µm 80, 100Å 220, 200m 2 /g — — No 

Weak Anion-2 (ion exchange) Silica Spherical 7µm 300Å 100m 2 /g — — No 

Strong Cation-1 (ion exchange) Silica Spherical 5, 10µm 80, 100Å 220, 200m 2 /g — — No 

*Available only online. 





Columns for Small Molecules (continued) 

Base 

Material 

Particle 


Carbon 

Load 

Endcapped? 

USP 

L-code 

Brand 

Phase 

Phase Type 

Grom Sil (cont.) Weak Cation-2 (ion exchange) Silica Spherical 7µm 300Å 100m 2 /g — — No 

HIC (hydrophobic interaction chrom.) Silica Spherical 7µm 300Å 100m 2 /g — — No 

Grom Sapphire C18 Silica Spherical 3, 5, 10µm 65, 110Å 500, 270m 2 /g 23, 16% Monomeric Yes L1 

C8 Silica Spherical 3, 5, 10µm 65, 110Å 500, 270m 2 /g 15, 10% Monomeric Yes L7 

C4 Silica Spherical 3, 5, 10µm 65, 110Å 500, 270m 2 /g 10.5, 7% Monomeric Yes L26 

Silica Silica Spherical 3, 5, 10µm 65, 110Å 500, 270m 2 /g — — No L3 

Mixed Mode C18/Cation Silica Spherical 5, 7µm 100Å 350m 2 /g — Polymeric No — 

C8/Anion Silica Spherical 7µm 100Å 350m 2 /g — Polymeric No — 

C8/Cation Silica Spherical 5µm 100Å 350m 2 /g — Polymeric No — 

Platinum C18 Silica Spherical 1.5, 3, 5µm 100Å 200m 2 /g 6% Monomeric Yes L1 

C18 EPS Silica Spherical 1.5, 3, 5µm 100Å 200m 2 /g 5% Monomeric No L1 

C8 Silica Spherical 1.5, 3, 5µm 100Å 200m 2 /g 4% Monomeric Yes L7 

C8 EPS Silica Spherical 3, 5µm 100Å 200m 2 /g 2.5% Monomeric No L7 

Phenyl Silica Spherical 3, 5µm 100Å 200m 2 /g — Monomeric Yes L11 

Cyano Silica Spherical 3, 5µm 100Å 200m 2 /g — Monomeric No L10 

Amino (NH2) Silica Spherical 3, 5µm 100Å 200m 2 /g — Monomeric No L8 


SAX Silica Spherical 3, 5µm 100Å 200m 2 /g — Monomeric No — 

Prevail C18 Select Silica Spherical 3, 5µm 110Å 350m 2 /g 17% Monomeric Yes L1 



Phenyl Silica Spherical 3, 5µm 110Å 350m 2 /g 7% Monomeric Yes L11 

Cyano Silica Spherical 3, 5µm 110Å 350m 2 /g — Monomeric Yes L10 



Organic Acid Silica Spherical 3, 5µm 110Å 350m 2 /g — Monomeric Yes — 

Carbohydrate ES Polymer Spherical 5µm — — — — No — 

Vydac ® SP 101SP Sil Silica Spheroidal 5, 10µm 100Å 250–350m 2 /g — unbonded No L3 

201SP C18 Silica Spheroidal 3, 5, 10, 15µm 90Å 250–350m 2 /g 13% Monomeric Yes L1 

208SP C8 Silica Spheroidal 5, 10, 15µm 90Å 250–350m 2 /g 9% Monomeric Yes L7 

VisionHT C18 Silica Spherical 1.5µm 100Å 200m 2 /g 6% Monomeric Yes L1 

C-18-B Silica Spherical 1.5µm 120Å 220m 2 /g 5.5% Monomeric † L1 

C18-P Silica Spherical 1.5µm 100Å 200m 2 /g 5% Monomeric No L1 

C18-HL Silica Spherical 1.5µm 120Å 220m 2 /g 11% Polymeric † L1 

HILIC, Silica Silica Spherical 1.5µm 120Å 220m 2 /g — — No L3 

Columns for Large Molecules 

Base 

Material 

Particle 


Carbon 

Load 

Endcapped? 

USP 

L-code 

Brand 

Phase 

Phase Type 

Genesis ® 300 C18 Silica Spherical 4, 7µm 300Å 120m 2 /g 10% Monomeric Yes L1 


CN Silica Spherical 4µm 300Å 120m 2 /g 3.3% Monomeric Yes L11 

Macrosphere 300 C18 Silica Spherical 5, 7µm 300Å 100m 2 /g 10% Monomeric Yes L1 


C4 Silica Spherical 5, 7µm 300Å 100m 2 /g — Monomeric Yes L26 

SAX Silica Spherical 7µm 300Å 100m 2 /g — Monomeric No — 

WAX Silica Spherical 7µm 300Å 100m 2 /g — Monomeric No — 

SCX Silica Spherical 7µm 300Å 100m 2 /g — Polymeric No — 

WCX Silica Spherical 7µm 300Å 100m 2 /g — Polymeric No — 

Macrosphere GPC GPC 60 Silica Spherical 7µm 60Å 450m 2 /g — Polymeric No L25 

GPC 100 Silica Spherical 7µm 100Å 350m 2 /g — Polymeric No — 



ProSphere C18 Silica Spherical 3, 5, 10µm 300Å 120m 2 /g 9% Monomeric Yes L1 

C18-AQ Silica Spherical 3, 5µm 100Å 450m 2 /g 18% Monomeric Yes L1 


Size-Exclusion 125 Silica Spherical 4, 5µm 125Å — — — No — 

Size-Exclusion 250 Silica Spherical 4, 5µm 250Å — — — No — 

Size-Exclusion 450 Silica Spherical 8µm 450Å — — — No — 

P-HR (reversed phase) Polymer Spherical 4µm 140Å — — — No — 

ProZap C18 ProZap Silica Spherical 1.5µm 500Å 59m 2 /g 3% Monomeric Yes L1 

Vydac ® ATP 214ATP C4 Silica Spheroidal 5, 10–15µm 300Å 70–110m 2 /g 3% Polymeric Yes L26 

Vydac ® TP 101TP Sil Silica Spheroidal 5, 10, 10–15, 15–20µm 300Å 70–110m 2 /g — unbonded No L3 

201TP C18 Silica Spheroidal 5, 7, 10, 10–15, 300Å 70–90m 2 /g 8% Polymeric No L1 

15–20µm 

202TP C18 Silica Spheroidal 3, 5, 10µm 300Å 60–90m 2 /g 9% Polymeric No L1 

208TP C8 Silica Spheroidal 3, 5, 7, 10, 10–15, 300Å 60–110m 2 /g 5% Polymeric Yes L7 

15–20µm 


15–20µm 


15–20µm 

219TP Di-Phe Silica Spheroidal 3, 5, 7, 10, 10–15, 300Å 60–110m 2 /g 4% Polymeric Yes 

15–20µm 

238TP C18 Silica Spheroidal 3, 5, 7, 10, 10–15, 300Å 60–110m 2 /g 4% Monomeric Yes L1 

15–20µm 

Everest ® 238EV C18 Silica Spherical 5, 10, 10–15, 15–20µm 300Å 70–110m 2 /g 6% Monomeric Yes L1 

Vydac ® MS 208MS C8 Silica Spheroidal 5µm 300Å 70m 2 /g 5% Polymeric Yes L7 

214MS C4 Silica Spheroidal 5µm 300Å 70–110m 2 /g 3% Polymeric Yes L26 

218MS C18 Silica Spheroidal 3, 5, 10, 10–15µm 300Å 60–110m 2 /g 8% Polymeric Yes L1 

238MS C18 Silica Spheroidal 5µm 300Å 70m 2 /g 4% Monomeric Yes L1 

219MS Di-Phe Silica Spheroidal 5µm 300Å 70m 2 /g 4% Polymeric Yes 

*Product information is available at www.discoverysciences.com. †Proprietary. 

30 



Grace ® HPLC Hardware Formats 

Grace Davison Discovery Sciences provides hardware formats to maximize the performance of the phases in our column families 

for all applications. Choose a hardware format based on your method requirements for speed, sensitivity, and resolution. 

Ultra High-Pressure Hardware 

Analytical Hardware 

Ideally suited for ultra high-pressure systems, this ultralow 

volume hardware is pressure rated to 18,000psig, 

and packed with 1.5µm media to maximize speed and 

efficiency. 

Lengths 

20, 30, 50, 100mm 

Expedite MS Hardware 

Inner 

Pressure Wetted 

Diameters Limit 

Surfaces 

1.0, 2.0mm 18,000psig 316 Stainless Steel 

High-speed, low-volume columns for rapid resolution and 

high-throughput LC/MS applications. 

7135 

6113 

4.6mm i.d. columns for standard HPLC instrumentation, 

the most commonly used. Analytical columns have 

industry standard port configurations. Some column 

families are also available with port configurations for 

Waters ® endfittings. 

Lengths 

30, 50, 100, 150, 

250, 300mm 

Metal-Free Hardware 

Inner 



Surfaces 

4.6mm 10,000psig 316 Stainless Steel 

7345 


Lengths 

10, 20mm 

Rocket Hardware 

Inner 



Surfaces 

2.1, 4.6mm 10,000psig 316 Stainless Steel 

High-speed, high-resolution columns for high-throughput 

analysis. Large 7mm i.d. balances column volume 

with system volume to deliver excellent peak shapes 

on conventional HPLC instrumentation. Large i.d. also 

allows fast mobile-phase flow rates which minimizes 

peak broadening. 

Lengths 

33, 53mm 

Solvent-Reducer and Microbore Hardware 

Smaller diameter columns reduce solvent consumption 

and increase sensitivity when compared to standard 

4.6mm i.d. columns. Use with standard HPLC 

instrumentation or with MS and ELS detectors. 

Lengths 

50, 100, 150, 150, 

250, 300mm 

Inner 



Surfaces 

7mm 10,000psig 316 Stainless Steel 

Inner 

Diameters 

Pressure 

Limit 

Wetted 

Surfaces 

1.0, 2.1, 3.0mm 10,000psig 316 Stainless Steel 

7345 

6904 

Mechanically strong, metal-free columns offer 

biocompatibility, and chemical resistance that ion 

chromatography and biotechnology applications demand. 

Lengths 

50, 100, 150, 250, 

300mm 

Capillary Guards 

Inner 

Pressure 


2.1, 4.6mm 5000psig PEEK 

All-Guard Guard Cartridges 

Wetted 

Surfaces 

Capillary guards offer zero dead-volume with finger-tight 

connections to maintain column performance. Use them 

to protective your capillary investment, as enrichment 

columns, or as short analytical columns. 

Lengths 

5, 10, 20mm 

Inner 

Diameters 

Pressure 

Limit 

Wetted 

Surfaces 

0.3, 0.5mm 5000psig 316 Stainless Steel 

and fused silica 

Guard system with reusable holder and disposable guard 

columns to protect analytical columns. 

6617 

7341 

6931 

Capillary Hardware 

Lengths 

7.5mm 

Inner 



Surfaces 

2.1, 3.0, 4.6mm 5000psig 316 Stainless Steel 

7344 

For LC/MS and other high-sensitivity and sample-limited 

applications. 

Lengths 

50, 100, 150, 250, 

300mm 

Inner 

Diameters 

0.075, 0.150, 

0.3, 0.5, 0.8mm 

Pressure 

Limit 

5000psig 

Wetted 

Surfaces 

316 Stainless Steel 

and fused silica 

more info 

Looking for preparative HPLC hardware? 

See page 150. 




High Throughput HPLC 

Introduction 

The Benefits of Grace ® Small Particles and 

Formats 

Today’s laboratories are under greater demand to analyze 

more samples in less time. To help meet this demand, major 

product advances have been made to decrease analysis 

time and increase HPLC throughput. HPLC system advances 

have greatly reduced the influence of extra column volumes 

and have extended the range of flow rates and pressure 

capabilities. In response to this, Grace now offers a wide 

range of column chemistries and formats appropriate for 

all types of high throughput systems. 

The use of smaller particles offers two main improvements to 

the chromatographic separation—increased resolution and 

speed. Resolution is directly proportional to the square root 

of column efficiency, therefore the higher the efficiency, the 

narrower the peaks and the greater the resolution between 

them. Increased speed comes from higher mobile phase flow 

rates that can be used without loss in efficiency, and higher 

flow rates mean faster analysis times. 

Understanding the type of system currently in use for 

high throughput separations and then matching the right 

column configuration is critical to achieving the best high 

throughput separation. Here we outline the three types of 

systems currently in use today for high throughput and the 

recommended HPLC column format for use with that system. 

System Type 1 

Ultra High-Pressure LC System 

(>10,000psig pressure limitation) 

Examples: Agilent 1200, Waters ® Acquity ® , Thermo Accela, 

Jasco XLc. 

Speed from Ultra High-Pressure Systems: 

Representing the latest in LC instrumentation technology, 

ultra high-pressure systems theoretically have the potential 

to deliver the fastest separations. They have minimal system 

volume and offer a pressure limit upwards of 12,000psig. 

This allows the use of columns with sub 2µm particles and 

2–7 times traditional flow rates. Sub 2µm particles extend the 

working range of acceptable mobile phase linear velocities 

without sacrificing efficiency. Therefore, you can push flow 

rates and still get equal or better performance. 

Suggested Column Format: 

VisionHT columns are designed for microbore and ultra 

high-pressure LC systems that have small system volumes 

to limit peak broadening from sample diffusion. VisionHT 

columns are packed with highly efficient 1.5µm phases 

that yield taller peaks and increase sensitivity. The column 

hardware incorporates a low dead volume design to minimize 

sample diffusion, and maintain peak integrity and efficiency. 

12,000psig high-pressure stability allows fast flow rates, 

decreasing run times 10 fold. 

System Type 1 

Recommended Column Format: 

Length: 

i.d.: 

VisionHT 

20, 30, 50, 100mm 

1.0 and 2.0mm 

tech tip 

Converting Methods From Traditional Column 

Formats to High Throughput Columns 

7135 

Convert Between Standard HPLC and VisionHT Columns 

Flow Rate 

Standard HPLC (4.6mm) 1.0X 

VisionHT (2.0mm) 2.3X 

When adjusting between standard LC conditions to VisionHT 

columns convert flow rates accordingly and then increase flow 

rate for faster analysis. 

Convert Between Standard HPLC Columns and Rocket Columns 

Flow Rate 

Standard Analytical (4.6mm) 1.0X 

Rocket Column (7.0mm) 2.3X 

mAU 

20 

15 

10 

5 

0 

VisionHT Columns for 

Ultra High-Pressure LC 

2 

5 

1 3 4 6 7 

8 

10724 

1. Sulfadiazine 

2. Sulfathiazole 

3. Sulfamerazine 

4. Sulfamethazine 

5. Sulfamethoxazole 

6. Sulfaisoxazole 

7. Sulfaquinoxilone 

8. Sulfamethoxine 

Use this conversion of flow rate to transfer methods between 

Rocket column or VisionHT columns. Backpressure on 

standard LC systems should be considered. 

0 1 2 3 4 5 Min. 

System Agilent 1200 

Mobile Phase: A: 0.1% Formic Acid 

B: Methanol 

Detector: UV at 280nm 

Temperature: Ambient 

more info 

See pages 34–37 for more information on VisionHT columns. 

32 



System Type 2 

Low Volume, High Throughput (HTP) 

LC System 

(

0 

857 

1714 

2571 

3428 

4285 

5142 

6000 

6856 

7713 

8570 

9427 

10284 

11141 

11998 

12855 

13712 

14569 

15426 

16283 

17140 


hplc columns | small molecule 

Grace ® VisionHT Ultra High-Pressure Columns 

See Separations with Speed and Clarity 

• Ultra-fast separations with superior efficiency, sensitivity, and resolution 

• Exceptional stability for long column lifetimes 

• Comprehensive sub 2μm stationary phase offering 

• 12,000psig pressure rating compatible with all ultra high-pressure LC systems 

Grace ® VisionHT columns offer a new level of performance in HPLC. The powerful combination of 

high-strength 1.5µm media with ultra-low volume hardware, delivers new clarity to your separations 

and maintains exceptional column lifetime. Complex samples resolve 95% faster with 4x greater 

sensitivity when compared to traditional 2.1 x 150mm, 5µm columns. And with a wide variety of 

phases available, the possibilities are endless. 

2500 mAU 

2000 

1500 

1000 

500 

2500 mAU 

0 

0 

2000 

0 

0 

2 

1500 

1 

3 

1000 

4 

500 

1 2 

2 

3 

2 4 6 8 10 12 14 Min. 

Increase Speed by 95% and Gain 4x Better Sensitivity 

Column: VisionHT C18, 1.5µm, 

2.0 x 50mm 


4 

2500 mAU 

2000 

1500 

1000 

4 6 8 10 12 14 Min. 

Column: Traditional C18, 5µm, 2.1 x 150mm 


500 

0 

1 

2 

3 

4 

Impurities 

not seen on 

traditional 

5µm columns. 

0 0.5 1 1.5 2 Min. 

1. Thiourea 

2. Toluene 

3. Propylbenzene 

4. Butylbenzene 

System: Agilent 1200 

Mobile Phase: (45:55) 20mM Potassium Phosphate, 

pH 2.5:Acetonitrile 



Efficiency and Retention Times Remain Constant after Exposure to 12,000psig and 2000 Injections 

Plates/Meter (000) 

300 

250 

200 

150 

100 

50 

Column Stability Comparison, 50 x 2mm 

VisionHT Column 1 




Column Volumes 




Competitor Sub 2µm Column 

Competitor 

Sub 2µm Column 

1 

2+3 

1 

2+3 

4 

4 6 

5 

5 

0 2 4 6 8 Min. 

6 

1. Uracil 

2. Pyridine 

3. Phenol 

4. Methyl Alanine 

5. 4-Butyl Benzoic Acid 

6. Toluene 

Initial 

FINAL 

(After 2000 injections) 

0 2 4 6 8 Min. 

Column: 2.0 x 50mm 

Mobile Phase: 50mM KH2PO4, pH 7.0:CH3CN (60:40) 


High-pressure competitor sub 2µm column lost efficiency at routine 

12,000psig pressures. VisionHT columns remain stable. 

Before and after chromatograms show a constant level of 

performance after 2000 injections. 

34 




Comprehensive High Purity 1.5µm Selectivity Options 

Sub 2µm particles deliver efficiency and speed, but critical to success is 

having the right stationary phase selectivity. Six VisionHT high purity phases 

are available, each with unique separation benefits. C18-HL, with maximum 

bonded phase coverage, is ideal for complex hydrophobic samples. Use C18-B 

for basic compounds at neutral pH, often a requirement for mass spec work in 

the pharmaceutical industry. Reserve the C18 and C18-P for fastest analysis 

times. Both offer increased polar interactions to make neutral, non-polar 

compounds elute faster and retain polar compounds longer. The HILIC and 

Silica packings are normal phases that typically use near exclusive organic 

mobile phases; an advantage when seeking highest mass spec sensitivity. 

VisionHT phases have exceptionally rigid silica structure that withstands 

routine use of 12,000psig pressure often required in this new technology. 

Reproducible silica synthesis, bonding and column packing guarantee 

low bleed and excellent column-to-column consistency. 

VisionHT Phase Specifications 

Base 

Material 

Spherical 

Particle Carbon Pore Surface 

Size Load Size Area Endcapped 

1.5µm 10% 120Å 220m 2 /g Yes 1–10 Fully bonded silica, ultra 

pH 

Range* Feature Benefits 

Packing 

C18-HL 

High capacity for hydrophobic compounds. Good 

Silica 

high purity silica peak symmetry. 

C18-B Spherical 1.5µm 5% 120Å 220m 2 /g Proprietary 1–10 High purity silica and Improved performance of basic compounds at 

Silica 

unique endcapping. neutral pH. Better sensitivity and peak shape by 

mass spec for basic compounds, without the need 

for acidified mobile phases. 

C18 Spherical 1.5µm 6% 100Å 200m 2 /g Yes 1–10 Moderate silica exposure Classic reversed-phase selectivity. Reduced 

Silica 

bonding is optimized for speed and sensitivity. 

C18-P Spherical 1.5µm 5% 100Å 200m 2 /g No 1–10 High silica exposure, low Unique polar selectivity. Low carbon load gives 

Silica 

carbon load 

fastest reversed-phase elution times while retaining 

HILIC 

Silica 

Spherical 

Silica 

Spherical 

Silica 

*Choice of buffer is critical at pH >8. 

1.5µm NA 120Å 220m 2 /g No 2–8 Polar phase with shorter 

equilibration times. 

Shipped in ACN/Water. 

1.5µm NA 120Å 220m 2 /g No 2–8 Traditional normal phase 

for use in 100% organic 

mobile phases 

polar compounds longer. 

Peak reversal compared to reversed-phase. Ideal 

for very polar compounds with high organic mobile 

phases for improved sensitivity by MS. 

For isomeric separation of non-aqueous compatible 

compounds by absorption chromatography. 

7443 


k' 

10 

9 

8 

7 

6 

5 

4 

3 

2 

1 

0 

VisionHT Reversed-Phase 

VisionHT Selectivity 

Selectivity 

k' Phenol 

k' Methyl Alanine 

k' Butyl Benzoic Acid 

k' Toluene 

C18-P 

C18 

C18-B 

VisionHT Phase 

C18-HL 





Separate Highly Basic Components at 

Neutral pH with Vision HT C18-B 

1 

2 

3 

1. Phenylephrine 


3. Amitriptyline 

0 1 2 3 4 5 Min. 

Column: VisionHT C18-B, 1.5µm, 2.0 x 50mm 

Mobile Phase: 50mM Ammonium Formate pH 7:Methanol (20:80) 








Unique Column Hardware Advantages 

You can erode the benefits of sub 2µm media if packed in 

an inefficient column design. VisionHT hardware uses 

thin screens, instead of traditional frits, to retain media and 

minimize dead volume. A unique insert combination seals 

endfittings leak-free to 18,000psig. Various dimensions offer 

even more flexibility over analysis speed and resolution. 

VisionHT Hardware Specifications 

Dead volume: 



Protect Your UHPLC Column Investment 

The smaller diameters and finer porosity frits required in 

UHPLC creates a higher likelihood of column and system 

blockage. Trace contaminates not normally problematic 

for traditional LC, may now pose backpressure issues. 

VisionHT guards protect your column to minimize down 

time and reduce cost. 

Two guard formats available: Integral and stand-alone. 

Couple the zero-dead volume integral guard directly to 

any VisionHT column, and realize absolutely no loss in 

efficiency. Use the stand-alone version to protect other 

manufacturers’ UHPLC columns, or as an on-line enrichment 

device. 

Prevent Column Blockage with Integral Guards 

0 0.5 1.0 1.5 2.0 Min. 

No loss in efficiency. 

VisionHT Integral Guard System 

N=174K/m 

with guard 

without guard 

Replace existing endfitting with the integral guard endfitting. Change 

guard cartridges as necessary. 

7456 

VisionHT Ultra High-Pressure Columns 

Packing Format i.d. x Length Part No. 

C18-HL, 1.5µm Ultra High-Pressure 1 x 20mm 5142540 

Ultra High-Pressure 1 x 30mm 5142541 







C18-B, 1.5µm Ultra High-Pressure 1 x 30mm 5141903 








C18, 1.5µm Ultra High-Pressure 1 x 20mm 5139555 








C18-P, 1.5µm Ultra High-Pressure 1 x 20mm 5139556 








HILIC, 1.5µm Ultra High-Pressure 1 x 20mm 5141910 








SI, 1.5µm Ultra High-Pressure 1 x 50mm 5141921 





Stand-Alone Guard System 

In-line guard system expands applications beyond column protection. 

Use as a sample enrichment device or to protect any uhplc 

commercially available column. 

7457 

VisionHT Guard Cartridges 

Packing Format i.d. x Length Qty. Part No. 

C18HL, 1.5µm Ultra High-Pressure 1 x 5mm 3/pk 5142549 

Ultra High-Pressure 2 x 5mm 3/pk 5142548 

C18 B, 1.5µm Ultra High-Pressure 1 x 5mm 3/pk 5141953 


C18, 1.5µm Ultra High-Pressure 1 x 5mm 3/pk 5141950 


C18-P, 1.5µm Ultra High-Pressure 1 x 5mm 3/pk 5141951 


HILIC, 1.5µm Ultra High-Pressure 1 x 5mm 3/pk 5141955 


SI, 1.5µm Ultra High-Pressure 1 x 5mm 3/pk 5141957 


Integral Guard Column Holder For VisionHT 3118351 

Stand-Alone Guard Holder 3118350 




Alltech ® Alltima HP Introduction 

High-Stability, High-Purity, High-Performance, Low-Bleed Columns for 

Demanding Applications 

• Better Peak Symmetry—high-purity silica eliminates peak tailing problems 

• Long Column Life—exceptional column stability minimizes downtime and reduces cost 

• Ideal for Microbore or Critical Analysis—low to no detectable column bleed 

• Variety of Phases and Formats—optimizes retention, resolution, and analysis time 

Alltima HP combines our best phase chemistries with high-purity silica. The result is 

one product family with selectivity and performance needed to overcome your most challenging 

separation needs. 

Alltech ® Alltima HP Phase Specifications 

Pore 

Size 

Surface 

Area 

Carbon 

Load 

Endcapped? 

Phase Description Base Particle Particle Size 

Phase 

USP 

C18 For classic reversed-phase applications Silica Spherical 3, 5µm 190Å 200 m 2 /g 12% Monomeric Yes L1 

C18 EPS Reversed-phase C18 with extended polar selectivity Silica Spherical 3, 5µm 190Å 200 m 2 /g 4% Monomeric Yes L1 

C18 HiLoad Higher carbon load for stronger retention Silica Spherical 3, 5µm 100Å 450 m 2 /g 24% Monomeric Yes L1 

C18 AQ 100% water wettable Silica Spherical 3, 5µm 100Å 450 m 2 /g 20% Monomeric Yes L1 

C18 Amide Low bleed polar-embedded phase compatible Silica Spherical 3, 5µm 190Å 200 m 2 /g 12% Monomeric Yes L1 

with microbore 

C8 For reversed-phase applications where C18 is Silica Spherical 3, 5µm 190Å 200 m 2 /g 8% Monomeric Yes L7 

too retentive 

Cyano Stable, long-life cyano phase Silica Spherical 3, 5µm 190Å 200 m 2 /g 4% Monomeric Yes L10 

Silica For general purpose normal-phase applications Silica Spherical 3, 5µm 100Å 450 m 2 /g — — No L3 

HILIC Hydrophillic Interaction Chromatography for highly 

polar analytes 

Silica Spherical 1.5, 3, 5µm 120Å 230 m 2 /g — — — L3 

Basic Compounds at Neutral pH 

1 

2 

0 1 2 3 4 5 6 7 Min. 

3 

The Alltima HP phases demonstrate excellent peak symmetry of highly basic analytes at pH 7.0. 

Peak 

USP Tailing* 

1. Diphenhydramine 1.29 

2. Nortriptyline 1.41 

3. Amitriptyline 1.27 

*Tailing Factor was determined in accordance with USP method. 

Column: Alltima HP C18, 5µm, 4.6 x 150mm 

Mobile Phase: 25mM Potassium Phosphate, pH 7.0:Methanol (15:85) 



Basic Compound Stability at pH1 and pH10 

1 

1 

2 

2 3 4 

2 3 4 

4 

31 

Final Acid 

Final Base 

Retention Times 

1. Uracil 

10249 

0.56 

10250 

0.55 

10252 

0.55 

2. Phenol 0.82 0.81 0.81 

3. N,N-Diethyl-m-Toluamide 1.08 1.08 1.08 

4. Toluene 1.89 1.88 1.89 

Column: Alltima HP C18, 5µm, 4.6 x 50mm 




Final Acid: washed with 24,000 column volumes of Acetonitrile:Dilute Sulfuric Acid, 

pH 1.0 (50:50) at 60°C. 

Final Base: washed with 24,000 column volumes of Acetonitrile:Ammonium 

Hydroxide, pH 10 (50:50) at 20°C. 

Initial 

0 1 

2 3 Min. 

The Alltima HP phases demonstrate exceptional stability from pH 1.0 to 10. 

38 



Alltech ® Alltima HP Introduction 

The Alltima HP Family Demonstrates Low Bleed 

Alltima HP columns are among the lowest-bleed columns available, making them ideal for microbore applications. Our C18 

Amide polar-embedded phase has significantly lower column bleed than competitive amide columns. 

Relative Abundance Level (X10 5 ) 

14 

12 

10 

8 

6 

4 

2 

Column Bleed by ESI-MS — Alltima HP vs. Competitive Columns 

Alltech ® Alltima HP 

Competitors 

0.82 0.82 0.82 0.82 

1.2 

2.91 

2.52 

11.8 

13.2 


0 

No 

Column 

Alltima HP 

Silica 

C18 

Alltima HP 

Alltima HP 

C18 HiLoad 

C18 MS 1 

C18 MS 2 

Alltima HP 

C18 Amide 

C18 Amide 1 

C18 Amide 2 

Alltima HP Method Development Kits 

best 

value 

Three Different Kits with Different Selectivities 

Whether you are developing a new method, or just not 

satisfied with an existing separation, the Alltima HP family 

can help optimize your separation. Your best value for method 

development—purchase a kit and receive three columns for 

the price of two. 

6113 

6904 

7345 

Alltima HP Method Development Kits 

Description Includes: i.d. x Length Part No. 

Kit 1— C18 Mix, 3µm 

Expedite MS Kit 1 C18, C18 HiLoad, C18 Amide (87674, 87692, 87728) 2.1 x 20mm 87854 

Analytical Kit 1 C18, C18 HiLoad, C18 Amide (87668, 87686, 87722) 4.6 x 150mm 87850 

Rocket Kit 1 C18, C18 HiLoad, C18 Amide (87672, 87690, 87726) 7 x 53mm 87852 

Kit 2— Alternate Mix, 3µm 

Expedite MS Kit 2 C18 HiLoad, C8, CN (87692, 87746, 87764) 2.1 x 20mm 87864 

Analytical Kit 2 C18 HiLoad, C8, CN (87686, 87740, 87760) 4.6 x 150mm 87860 

Rocket Kit 2 C18 HiLoad, C8, CN (87690, 87744, 87762) 7 x 53mm 87862 

Kit 3 — Polar Mix, 3µm 

Expedite MS Kit 3 C18 EPS, C18 Amide, CN (87710, 87728, 87764) 2.1 x 20mm 87874 

Analytical Kit 3 C18 EPS, C18 Amide, CN (87704, 87722, 87758) 4.6 x 150mm 87870 

Rocket Kit 3 C18 EPS, C18 Amide, CN (87708, 87726, 87762) 7 x 53mm 87872 

tech tip 

Want high throughput on a conventional (non-ultra 

high-pressure) system? 

Choose the Rocket column format—no modifications 

necessary to your existing HPLC system. Choose Expedite 

columns for low volume and microbore systems. See page 31 

for more explanation of the Rocket and Expedite formats. 




40 

Alltech ® Alltima HP 

Reversed-Phase Columns 

Selectivity Options in One Product Family 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

C18: 

• Classic reversed-phase retention and selectivity 

C18 AQ: 

• 100% water wettable and high carbon loading for greater 

mobile phase range 

EPS C18: 

• Unique selectivity that succeeds when traditional 

reversed-phase columns fail 

• Greater retention and enhanced peak symmetry for polar 

compounds 

C18 HiLoad: 

• Superior retention and loadability for resolution of 

complex samples 

C18 Amide: 

• The first polar-embedded packing ideal for microbore 

• Excellent peak shape without phase bleed 

• Ideal for basic compounds in neutral to alkaline pH 

C8: 

• Less hydrophobic retention than C18 

 

 

 

 

Alltima HP Selectivity 

 

 

 

 

Alltima HP Packing 

 

12 

1 2 3 

Acid, Base, and Neutral Test Mix 

5 

3 

4 

5 

4 

1 2 

3 

6 

0 2 4 6 8 Min. 


Mobile Phase: 50mM KH2PO4, pH 3.0:CH3CN (40:60) 

Column Temp: Ambient (22°C) 


Injection Vol.: 5µL 



4 

6 

5 

6 

1. Uracil 

2. Pyridine 

3. Phenol 

4. N,N-diethyl-m-toluamide 

5. 4-Butyl Benzoic Acid 

6. Toluene 

C18 

EPS C18 

C18 HiLoad 

Alltima HP HPLC Columns 


C18, 3µm* Capillary 0.150 X 50mm 22153 

Capillary 0.150 X100mm 22154 


Capillary 0.300 X 50mm 22156 



Expedite MS 2.1 x 10mm 87673 


Microbore 2.1 x 50mm 87504 



Solvent-Reducer 3.0 x 150mm 87601 



Analytical 4.6 x 50mm 87826 



Rocket 7 x 33mm 87671 


C18, 5µm Microbore 2.1 x 150mm 87681 






C18 AQ, 3µm* Capillary 0.150 x 50mm 22560 

Capillary 0.150 x 100mm 22561 
















C18 AQ, 5µm Microbore 2.1 x 150mm 87821 




C18 EPS, 3µm Expedite MS 2.1 x 10mm 87709 













C18 EPS, 5µm Microbore 2.1 x 150mm 87717 






*Other particle sizes and dimensions are available.


Alltech ® Alltima HP Reversed-Phase Columns 

Alltima HP HPLC Columns (continued) 


C18 Hi-Load, Capillary 0.150 x 50mm 22190 

3µm* Capillary 0.300 x 50mm 22193 














C18 Hi-Load, Microbore 2.1 x 150mm 87699 

5µm Microbore 2.1 x 250mm 87700 





C18 Amide, Capillary 0.150 x 50mm 22255 

3µm* Capillary 0.300 x 50mm 22258 














C18 Amide, Microbore 2.1 x 150mm 87735 






C8, 3µm* Capillary 0.150 x 50mm 22479 

























*Other particle sizes and dimensions are also available. 

Alltima HP-Guard Cartridges 

Packing i.d. x Length Qty. Part No. 

C18 Capillary Guard, 3µm** 0.150 x 10mm ea 22578 

0.300 x 10mm ea 22579 

C18 All-Guard, 5µm* 2.1 x 7.5mm 3 87683 

3.0 x 7.5mm 3 87622 

4.6 x 7.5mm 3 87684 

C18 AQ Capillary Guard, 3µm** 0.150 x 10mm ea 22638 

0.300 x 10mm ea 22639 

C18 AQ All-Guard, 5µm* 2.1 x 7.5mm 3 87823 

4.6 x 7.5mm 3 87824 

C18 EPS All-Guard, 5µm* 2.1 x 7.5mm 3 87719 

3.0 x 7.5mm 3 87623 

4.6 x 7.5mm 3 87720 

C18 Hi-Load Capillary 0.150 x 10mm ea 22588 

Guard, 3µm** 0.300 x 10mm ea 22589 

C18 Hi-Load All-Guard, 5µm* 2.1 x 7.5mm 3 87701 

3.0 x 7.5mm 3 87624 

4.6 x 7.5mm 3 87702 

C18 Amide Capillary 0.150 x 10mm ea 22598 

Guard, 3µm** 0.300 x 10mm ea 22599 

C18 Amide All-Guard, 5µm* 2.1 x 7.5mm 3 87737 

3.0 x 7.5mm 3 87625 

4.6 x 7.5mm 3 87738 

C8 Capillary Guard, 3µm** 0.150 x 10mm ea 22648 

0.300 x 10mm ea 22648 


3.0 x 7.5mm 3 87627 

4.6 x 7.5mm 3 87756 

All-Guard Cartridge Holder 

ea 80101 

(Includes Direct-Connect Column Coupler) 

Capillary Guard Cartridge Holder 

Guard Holder for 0.100mm and 0.150mm Guards ea GR-3710E 

Guard Holder for 0.300mm and 0.500mm Guards ea GR-3710A 

*All-Guard holder required. 

**Other particle sizes and dimensions are available. 

tech tip 

Which guard format should I choose? 

Ideally, the guard should have the same or slightly smaller i.d. as 

the HPLC column. Efficiency will suffer, if the guard i.d. is larger 

than that of the column. 









Alltech ® Alltima HP HILIC Columns 

Hydrophilic Interaction (HILIC) Columns for 

Highly Polar Basic Analytes 

• Superior retention of highly polar compounds 

• Increased sensitivity and lower detection limits 

with microbore 

• Available in 1.5µm particle size 

Hydrophilic Interaction Chromatography is ideal for 

separating and retaining very polar compounds that may 

not retain on traditional reversed-phase packings. Unlike 

reversed-phase columns, HILIC columns retain highly polar 

compounds with only small amounts of water in the mobile 

phase. These more volatile mobile phases increase sensitivity 

with microbore applications. 

1 

2 

Basic Drugs 

Reverse-Phase 

C18 Columns 

10949 

1 

2 

3 

4 

0 1 2 3 4 5 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

5 

Mixed Analyte HILIC 

1. Toluene 

2. Hydantoin 

3. Uracil 

4. Caffeine 

5. Allantoin 

Alltima HP HILIC, 5μm, 4.6 x 150mm 

(Part No. 86464) 

Acetonitrile:0.10M Ammonium Formate, 

pH 3.0 (95:5) 

1.0mL/min 

UV at 205nm 

10461 

Alternate selectivity compared to reversed-phase columns allows 

more separation possibilities. 

2 

1 

Alltima HP 

HILIC Columns 

0 1 2 3 4 5 6 7 8 9 10 Min. 

1. Albuterol 

2. Bamethan 

Column: Prevail C18 Column, 5μm, 4.6 x 150mm 

(Part No. 99208) 

Alltima HP HILIC Column, 5μm, 4.6 x 150mm, 

(Part No. 86464) 

Mobile Phase: 0.5M Ammonium Formate, pH 3.0:CH3CN (88:12) 

Acetonitrile:0.5M Ammonium Formate, pH 3.0 (85:15) 



Use Alltima HP HILIC's alternate selectivity for greater separation 

possibilities. 

tech tip 





columns for low volume and microbore systems. See page 31 

for more explanation of the Rocket and Expedite formats. 

Rocket 

Columns 

6904 

Expedite 

Columns 

6113 

Alltima HP Hilic HPLC Columns 


HILIC, 1.5µm Expedite MS 2.1 x 20mm 86471 




HILIC, 3µm* Capillary 0.150 x 50mm 22515 
















HILIC, 5µm Microbore 2.1 x 150mm 86465 



*Other particle sizes and dimensions are available. 

Alltima HP HILIC Guard Cartridges 


HILIC Capillary Guard, 0.150 x 10mm ea 22658 

3µm** 0.300 x 10mm ea 22659 

HILIC All-Guard, 5µm* 2.1 x 7.5mm 3 86479 

4.6 x 7.5mm 3 86480 


ea 80101 






**Other particle sizes and dimensions are available. 

42 



Alltech ® Alltima HP Normal-Phase Columns 

Cyano: • Superior stability 

• More reproducible separations than silica for 

normal-phase applications 

• Ideal for basic drug analysis 

Silica: • Normal phase 

% Changes in Retention 

-35 

-30 

-25 

-20 

-15 

-10 

-5 

-0 

Alltima HP Cyano — 

Much More Stable than Other Cyanos 

Alltima HP CN 

pH 1.0 

pH 7.5 

Brand A CN 

Brand B CN 

Brand C CN 

Brand D CN 

Brand E CN 

Brand F CN 

QC D4 Test: 70:30 H2O/CH3CN,1.0mL/min, UV at 254nm, 5µL 

D4Mix, Column 4.6 x 150mm, 22°C 

pH 1.0 Stability: Flushed with H2SO4 pH 1.0:Acetonitrile 50:50 at 

1.0mL/min for 3 days 

pH 7.5 Stability: Flushed with 20mM K2HPO4 pH 7.5:Acetonitrile 

50:50 at 1.0mL/min for 3 days 

Alltima HP HPLC Columns 


Cyano, 3µm Expedite MS 2.1 x 10mm 87763 













Cyano, 5µm Microbore 2.1 x 150mm 87781 






Silica, 3µm Expedite MS 2.1 x 10mm 87791 













0 

1. Deoxycorticosterone Acetate 

2. Hydrocortisone Acetate 

3. Cortisone Acetate 

4. Cortisone 

1 

2 

Corticosteroids 

3 4 

5 

1 2 3 4 5 6 7 8 9 10 11 12 13 Min. 

Column: Alltima HP CN, 5μm, 4.6 x 150mm 

(Part No. 87769) 

Mobile Phase: Hexane:Isopropanol (93.7:6.3) 




6 

5. Prednisone 

6. Hydrocortisone 

7. Prednisolone 

7 

10391 

Alltima HP HPLC Columns (continued) 


Silica, 5µm Microbore 2.1 x 150mm 87799 






Alltima HP All-Guard Cartridges* 


Cyano, 5µm 2.1 x 7.5mm 3 87783 

3.0 x 7.5mm 3 87626 

4.6 x 7.5mm 3 87784 

Silica, 5µm 2.1 x 7.5mm 3 87803 

3.0 x 7.5mm 3 87628 

4.6 x 7.5mm 3 87804 


ea 80101 


*All-Guard holder required. Other particle sizes available. 









Alltech ® Prevail Introduction 

Stable from 100% Organic to 100% Aqueous 

• Long column life in both highly aqueous and highly organic mobile phases 

• Excellent retention and reproducibility of highly polar analytes in 100% aqueous 

mobile phases 

• Strong retention of hydrophobic analytes in 100% organic mobile phases, no concerns 

of solubility, better sensitivity in microbore and ELSD applications 

• No phase collapse 

• Specialty phases for specific applications 

With one Prevail C18 column, retain polar compounds in aqueous mobile phases and hydrophobic compounds in organic 

mobile phases. 

Alltech ® Prevail Phase Specifications 

Phase Base Material Particle Shape Particle Size Pore Size Surface Area Carbon Load Phase Type Endcapped? USP L-code 

C18 Select Silica Spherical 3, 5µm 110Å 350m 2 /g 17% Monomeric Yes L1 



Phenyl Silica Spherical 3, 5µm 110Å 350m 2 /g 7% Monomeric Yes L11 

Cyano Silica Spherical 3, 5µm 110Å 350m 2 /g — Monomeric Yes L10 


Silica Silica Spherical 3, 5µm 110Å 350m 2 /g — — — L3 

Organic Acid Silica Spherical 3, 5µm 110Å 350m 2 /g — Monomeric Yes — 

Carbohydrate ES Polymer Spherical 5µm — — — — — — 

Switch Between 100% Aqueous and 100% Organic Mobile Phases on the Same Column 

Water-Soluble Vitamins 

(100% Aqueous) 

9465 

Fat-Soluble Vitamins and Tocopherols 

(100% Organic) 

9405 


(100% Aqueous) 

9465 

3 

1. Thiamine 

2. Ascorbic Acid 

3. Nicotinic Acid 

4. Pyridoxine 

5. Niacinamide 

6. Pantothenic Acid 

1. Vitamin A 

2. δ-Tocopherol 

3. γ-Tocopherol 

4. α-Tocopherol 

(Vitamin E) 

5. Vitamin D2 

6. Vitamin D3 

7. Vitamin K 

4 

6 

3 

1. Thiamine 



4. Pyridoxine 



1 

2 

4 5 

6 

wash with 

intermediate 

1 

2 

3 

5 

7 

wash with 

intermediate 

1 

2 

4 5 

6 

0 2 4 6 8 10 12 14 Min. 

0 2 4 

6 

8 10 12 14 Min. 

0 2 4 6 8 10 12 14 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Prevail C18, 5µm, 

4.6 x 150mm 

25mM KH2PO4 

pH 3.0:CH3CN (97:3) 

1.0mL/min 

UV at 212nm 

Column: Prevail C18, 5µm, 

4.6 x 150mm 

Mobile Phase: CH3CN:CH3OH (75:25) 



Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Prevail C18, 5µm, 

4.6 x 150mm 

25mM KH2PO4 

pH 3.0: CH3CN (97:3) 

1.0mL/min 

UV at 212nm 

Figure 1 

Figure 2 

Figure 3 

Prevail Columns have highly stable bonded phases that let you use one column for multiple mobile phase conditions. Even switching between 

extremes such as near 100% aqueous (Figure 1) to 100% organic conditions (Figure 2), and back to near 100% aqueous (Figure 3) on a routine 

basis is possible, provided that mobile phases are miscible. 

44 



Alltech ® Prevail Reversed-Phase Columns 

Prevail HPLC Columns 


Select C18, Microbore 2.1 x 50mm 99309 








Select C18, Microbore 2.1 x 150mm 99310 






C18, 3µm Microbore 150µm x 50mm 35762 

Microbore 150µm x 150mm 35764 


















tech tip 





columns for low volume and microbore systems. See page 31 for 

more explanation of the Rocket and Expedite formats. 

Prevail HPLC Columns (continued) 


C18, 5µm Microbore 150µm x 50mm 35792 




















Phenyl, 3µm Microbore 1.0 x 100mm 43893 















Phenyl, 5µm Microbore 2.1 x 150mm 99237 






9450 

9451 

9452 

Prevail Bonded Phases are Stable in 100% Aqueous Conditions 

Prevail C18, 5µm, 4.6 x 150mm 

Competitor A C18, 5µm, 4.6 x 150mm 

1 

Catecholamines 

1 2 

1. Norepinephrine 

1 

2 

2. Epinephrine 

3. 5-Hydroxydopamine 

1 

2 

3 

Mobile Phase: 25mM KH2PO4, pH 2.7 



After 24 hrs. 

After 8 hrs. 

After 20 min. 

3 

3 

2 

3 

9453 

9454 

9455 

0 1 2 3 4 5 6 Min. 

0 1 2 3 4 5 6 Min. 




Alltech ® Prevail Normal-Phase Columns 













Amino, 3µm Analytical 4.6 x 50mm 43926 





Silica, 3µm Microbore 1.0 x 100mm 43832 















Prevail HPLC Columns (continued) 


Silica, 5µm Analytical 2.1 x 150mm 99273 





Prevail All-Guard Cartridges* 


Select C18, 5µm 2.1 x 7.5mm 3 96690 

3.0 x 7.5mm 3 99119 

4.6 x 7.5mm 3 96455 

C18, 5µm 2.1 x 7.5mm 3 96682 

3.0 x 7.5mm 3 99350 

4.6 x 7.5mm 3 99286 

C8, 5µm 2.1 x 7.5mm 3 99128 

3.0 x 7.5mm 3 99351 

4.6 x 7.5mm 3 99287 

Phenyl, 5µm 2.1 x 7.5mm 3 99130 

3.0 x 7.5mm 3 99352 

4.6 x 7.5mm 3 99288 

Cyano, 5µm 2.1 x 7.5mm 3 99131 

3.0 x 7.5mm 3 99353 

4.6 x 7.5mm 3 99289 

Amino, 5µm 4.6 x 7.5mm 3 99290 

Silica, 5µm 2.1 x 7.5mm 3 99133 

3.0 x 7.5mm 3 99354 

4.6 x 7.5mm 3 99291 

All-Guard Cartridge Holder ea 80101 


Alltech ® Prevail Organic Acid Columns 

Get Unsurpassed Resolution for Common 

Organic Acids 

• Acid stable for long column lifetimes 

• Silica-based for maximum efficiency and high-resolution 

• Short run times and high sample throughput at ambient 

temperature with Rocket format 

• Lower cost than polymeric organic acid columns 

Prevail Organic Acid (OA) columns separate common 

organic acids with an unsurpassed combination of resolution, 

speed, sensitivity, and simplicity. A simple acidic phosphate 

buffer and a Prevail OA column at ambient temperature will 

separate 11 short-chain organic acids in less than 6 minutes. 

Use pH to adjust column selectivity. Lowering the mobile 

phase pH progressively suppresses the ionization of the 

carboxylic acids, making them more hydrophobic. This gives 

you the ability to move these peaks relative to other peaks in 

the chromatogram, and it simplifies method development. 

1 

2 

3 4 5 67 8 

Figure 1 - Organic Acids 

9 10 

11 

0 1 2 3 4 5 6 7 8 Min. 

Column: Prevail Organic Acid, 5µm, 4.6 x 150mm 

(Part No. 88640) 

Mobile Phase: 25mM KH2PO4, pH 2.5 



1. Oxalic Acid 

2. Tartaric Acid 

3. Formic Acid 

4. Malic Acid 

5. Lactic Acid 

6. Acetic Acid 

7. Diglycollic Acid 

8. Maleic Acid 

9. Citric Acid 

10. Succinic Acid 

11. Fumaric Acid 

Separate 11 short-chain organic acids in less than six minutes. 

9384 


Standard 

Part No. 

Waters ® 

Fittings 

Part No. 

Packing Format i.d. x Length 

OA, 3µm Microbore 2.1 x 100mm 88648 — 

Analytical 4.6 x 100mm 88650 88750 


Rocket 7 x 33mm 99292 — 

Rocket 7 x 53mm 50755 — 

OA, 5µm Analytical 4.6 x 150mm 88640 88740 




Organic Acid, 5µm 4.6 x 7.5mm 3 96429 



46 



Alltech ® Prevail Carbohydrate ES Columns 

Get Better Retention Times, Longer Column Life, and Improved Baselines Compared to 

Traditional Amino Carbohydrate Columns 

• Longer column life: Hybrid phase has the ruggedness of silica with the stability of a polymer 

• Quieter baselines compared to traditional amino carbohydrate columns, even with gradients 

• Reduce run times with more mobile phase choices 

• Compatible with gradient ELSD applications 

Quiet Gradient Baselines 

Prevail Carbohydrate ES Columns are excellent for 

isocratic separations with common detectors, but the 

column’s real power shows with the ELSD and a solvent 

gradient. Enjoy the powerful selectivity, reduced run times, 

and efficient peaks from gradients while seeing the quiet, 

stable baselines that maximize sensitivity. 

9354 

9379 

1 

2 3 

4 5 6 

7 

4 5 6 

10 11 13 

7 

8 9 12 

0 5 10 15 20 25 Min. 

1 

2 

3 

Enhanced Detector Sensitivity Through Flat Gradient Baselines 

12 

13 

14 

15 

18 19 20 21 22 23 24 Min. 

Silica Based 

Amino Column 

9486 

Prevail 

Carbohydrate ES Column 

(Part No. 35101) 

Better Resolution and Peak Shape 

Prevail Carbohydrate ES Columns are versatile 

enough for mono- and oligosaccharides and sugar alcohols. 

They produce single peaks for reducing sugars at ambient 

temperatures, eliminating the need for column heating. 

1. Dextrose 

2. Maltose 

3. Maltotriose 

4. Maltotetraose 

5. Maltopentaose 

6. Maltohexaose 

7. Maltoheptaose 

8. Maltooctaose 

9. Maltononaose 

10. Maltodecaose 

11. Maltoundecaose 

12. Maltododecaose 

13. Maltotridecaose 

14. Maltotetradecaose 

15. Maltopentadecaose 




%B: 35 50 


Detector: ELSD 


Use gradients with a Prevail Carbohydate ES column and the ELSD for shorter run times, better resolution, and greater sensitivity. 

By comparison, silica-based amino columns give noisy, shifting baselines. 

1 

2 

3 

4 

6 

5 

7 

Pepsi One ® Cola 

0 5 10 15 20 25 30 35 40 Min. 

8 

10530 

1. Sucralose 

2. Unknown 1 

3. Fructose 

4. Glucose 

5. Unknown 2 

6. Sucrose 

7. Maltose 

8. Maltodextrins 

Column: Prevail Carbohydrate ES, 5µm, 4.6 x 250mm 

(Part No. 35101) 



%B: 20 20 30 30 50 






Carbohydrate ES, Analytical 4.6 x 150mm 35102 

5µm Analytical 4.6 x 250mm 35101 


1 

2 4 

3 


1. Sucralose 

2. Fructose 

3. Sorbitol 

4. Glucose 

5. Sucrose 

6. Maltose 

5 6 

0 5 10 15 20 25 30 35 40 Min. 

10557 

Column: Prevail Carbohydrate ES, 5µm, 4.6 x 250mm 

(Part No. 35101) 



%B: 15 15 25 25 50 






Carbohydrate ES, 5µm 4.6 x 7.5mm 3 96435 





Alltech ® Platinum Columns 


For Challenging Separations 

• Unique selectivity 

• Better peak shapes with polar analytes 

• More separation choices with dual-selectivity 

• Excellent stability and reproducibility 

• 1.5µm high throughput media for speed and resolution, especially when combined with 

Rocket and Expedite hardware 

The Platinum Column Advantage 

Controlled silica exposure is the difference that makes Platinum columns unique. Instead of thoroughly 

covering the silica with bonded phase to hide the silica, the exposure of the silica in Platinum columns is controlled to provide 

a dual mode separation with both polar and non-polar sites exposed to your samples. This extends polar selectivity well beyond 

what other reversed-phase columns offer and gives separations other columns cannot. 

Standard Platinum Columns vs Platinum EPS Columns 

Platinum columns come in two varieties offering different levels of silica exposure. Standard Platinum has a moderate silica 

exposure and is best used with neutral and moderately polar compounds. Platinum EPS (Extended Polar Selectivity) has a 

high level of silica exposure and is best used with compounds containing more than two polar functional groups. 

Alltech ® Platinum Phase Specifications 



EPS C18 Silica Spherical 1.5, 3, 5µm 100Å 200m 2 /g 5% Monomeric No L1 


EPS C8 Silica Spherical 3, 5µm 100Å 200m 2 /g 2.50% Monomeric No L7 

Phenyl Silica Spherical 3, 5µm 100Å 200m 2 /g — Monomeric Yes L11 

Cyano Silica Spherical 3, 5µm 100Å 200m 2 /g — Monomeric No L10 


Silica Silica Spherical 3, 5µm 100Å 200m 2 /g — — — L3 

SAX Silica Spherical 3, 5µm* 100Å 200m 2 /g — Monomeric No — 

Trying to solve difficult separation problems using typical reversed-phase columns often leads to the same result. Choose Platinum columns 

for completely different selectivity. See chart below. 

Hydrophobic/Hydrophillic Balance at pH 3 of Various Reversed-Phase HPLC Packings 

1.6 

1.4 

1.2 

Platinum 

EPS C8 

Platinum EPS C18 

k’ Amitripyline / k’ Toluene 

1 

0.8 

0.6 

0.4 

0.2 

Standard 

Platinum C8 

Typical RP C8 

N O T E 

Platinum column’s unique selectivity 

stands out from the competition. 

Standard 

Platinum C18 

Typical RP C18 

0 

Typical Polar Embedded 

0 1 2 3 4 5 6 7 8 

k’ Toluene 

Plotting k' values of different compounds (polar vs. nonpolar) demonstrate the unique selectivity of Platinum and Platinum EPS columns, 

compared to conventional reversed-phase columns. 

48 



Reverse Elution Order with Standard 

Platinum and Platinum EPS Columns 

Often, it’s preferable when minor components elute before, 

rather than after, closely retained major components. 

1,2 

Standard Platinum C18 

2 

4 

0 2 4 6 8 10 12 14 16 Min. 

5 

Platinum EPS C18 

Vasodilators 

1 

3 

4 

3 

5 

8387 

1. Pindolol 

2. Pentoxifylline 

3. Isoxsuprine 

4. Nifedipine Degradant 

5. Nifedipine 

8388 

Column: 5µm, 4.6 x 150mm 

Mobile Phase: 0.025M KH2PO4, pH 3.0:Methanol (50:50) 



Platinum EPS Columns Have 

High Polar Compound Capacity 

This is important for early eluting polar compounds which are 

often unresolved on conventional reversed-phase columns. 

Capacity Factor 

Capacity Factors for Platinum EPS C18 Columns 

6.00 

5.00 

4.00 

3.00 

2.00 

1.00 

Biphenyl 

Amitriptyline 

Toluene 

Nitrobenzene 

Cinnamic Acid 

0.00 

1 5 10 15 20 25 30 35 40 45 

Consecutive Batches 


Platinum HPLC Columns 

Standard 

Part No. 

Waters ® Fittings 

Part No. 


C18, 1.5µm Rocket 7.0 x 33mm 50527 — 

Rocket 7.0 x 53mm 50529 — 

C18, 3µm Solvent Reducer 3.0 x 150mm 32794 — 



Rocket 7.0 x 33mm 50525 — 

Rocket 7.0 x 53mm 50523 — 

C18, 5µm Solvent Reducer 3.0 x 150mm 32793 — 

Solvent Reducer 3.0 x 250mm 32792 — 



EPS C18, 1.5µm Rocket 7.0 x 33mm 50577 — 

Rocket 7.0 x 53mm 50579 — 

EPS C18, 3µm* Capillary 0.150x 50mm 22300 — 

Capillary 0.150x100mm 22430 — 


Capillary 0.300x 50mm 22432 — 






Rocket 7.0 x 33mm 50575 — 

Rocket 7.0 x 53mm 50573 — 

EPS C18, 5µm Solvent Reducer 3.0 x 150mm 32806 — 




C8, 1.5µm Rocket 7.0 x 53mm 50529 — 

C8, 3µm Rocket 7.0 x 33mm 50532 — 

C8, 5µm Analytical 4.6 x 150mm 32370 32371 


EPS C8, 3µm Analytical 4.6 x 150mm 32415 32416 

Rocket 7.0 x 33mm 50583 — 

Rocket 7.0 x 53mm 50585 — 

EPS C8, 5µm Analytical 4.6 x 150mm 32420 32421 


*1.5µm and 5µm particles and other dimensions are available. 

Platinum HPLC Columns (continued) 

Standard 

Part No. 


Part No. 


Phenyl, 3µm Analytical 4.6 x 150mm 32631 32632 



Cyano, 3µm Rocket 7.0 x 33mm 50593 — 

Rocket 7.0 x 53mm 50595 — 

Cyano, 5µm Analytical 4.6 x 150mm 32672 32675 


Amino, 3µm Analytical 4.6 x 150mm 32706 32707 

Rocket 7.0 x 53mm 50545 — 



Silica, 3µm Analytical 4.6 x 150mm 32535 32536 



SAX, 3µm Analytical 4.6 x 150mm 32952 32953 



Platinum Guard Cartridges 



4.6 x 7.5mm 3 32606 

EPS C18, 3µm 

0.150 x 10mm — 22693 

Capillary Guard** 

0.300 x 10mm — 22694 

EPS C18 All-Guard, 5µm* 3.0 x 7.5mm 3 99117 

4.6 x 7.5mm 3 32607 


EPS C8 All-Guard, 5µm* 4.6 x 7.5mm 3 32614 

Phenyl All-Guard, 5µm* 4.6 x 7.5mm 3 32619 

Cyano All-Guard, 5µm* 4.6 x 7.5mm 3 32620 

Amino All-Guard, 5µm* 4.6 x 7.5mm 3 32621 

Silica All-Guard, 5µm* 4.6 x 7.5mm 3 32622 

SAX All-Guard, 5µm* 4.6 x 7.5mm 3 32787 


ea 80101 






**1.5µm and 5µm particles and other dimensions are available. 





High Quality HPLC Phases at Exceptional Value 

• High purity phases 

• Efficient and reproducible separations 

• General use selectivity 

• Exceptional value 

GraceSmart HPLC phases use high-purity silica and are monomerically bonded with uniform 

coverage. This translates into symmetrical peaks for acids/bases, and predictable reversed-phase 

selectivity. Whether routine analysis or new method development, use GraceSmart columns to 

get premium performance at exceptional value. 

Reproducible Methods Start with 

Reproducible Columns 

Our tightly controlled silica synthesis and bonding keep 

capacity factor and selectivity variations to a minimum. The 

advanced packing methods deliver consistently high columnto-column 

efficiency. Every column is individually tested and 

shipped with a quality assurance chromatogram. 

best 

value 

new 

GraceSmart Phase Specifications 

Phase Encapping Surface Area Pore Size Particle Size 

C18 TMS, non-polar 220m 2 /g 120Å 3, 5µm 

Expect Competitive Performance 

Six sigma techniques and lean manufacturing are key to our 

cost competitive position, without compromising quality or 

performance. GraceSmart columns show similiar or better 

efficiency and asymmetries for challenging base and acid 

components in comparison to industry leading columns. 

Capacity Factor 

5 

4.5 

4 

3.5 

3 

2.5 

2 

1.5 

1 

0.5 

0 

1 2 3 4 5 6 7 8 9 10 11 12 

Toluene 

N-Butylbenzoic Acid 

Anisole 

Highly Reproducible 

Batch 

Acetophenone 

P-Nitrophenol 

Amitriptyline 

Consistent batch-to-batch capacity for acidic, basic, and neutral 

compounds. 

Symmetry Plates / Columns 

12,000 

10,000 

8,000 

6,000 

4,000 

2,000 

0 

2.5 

2 

1.5 

1 

0.5 

0 

Nicotine 

Nicotine 

Efficiency 

Quinine 

Symmetry 

Quinine 

Diphenhydramine 

Diphenhydramine 

Competitor 1 C18 Column 



GraceSmart C18 Column 

GraceSmart columns compare favorably to leading competitor 

columns. 

Nortriptyline 

Nortriptyline 

2,4 Dichlorophenoxy Acetic Acid 

2,4 Dichlorophenoxy Acetic Acid 

2,4 Dichlorophenoxy Propionic Acid 

2,4 Dichlorophenoxy Propionic Acid 

2,4 Dichlorophenoxy Butyric Acid 

2,4 Dichlorophenoxy Butyric Acid 

GraceSmart RP18 Columns 


C18, 5µm Analytical 4.6 x 150mm 5138812 










50 




mAu 

6000000 

5500000 

5000000 

4500000 

4000000 

3500000 

3000000 

2500000 

2000000 

1500000 

1000000 

500000 

0 

-500000 

Amoxicillin (U.S.P.) 

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 Min. 


Luna C18 Column 

Inertsil ® ODS-3 Column 

Hypersil ® BDS C18 Column 

Kromasil ® 100-5 C18 Column 

10697 

Column: GraceSmart C18 Column, 5µm, 4.6 x 250mm 

(Part No. 5138810) 

Mobile Phase: Isocratic, 0.05N Monobasic Potassium Phosphate 

pH 5:Acetonitrile (96:4) 




Injection Vol: 10µL 

3 

1 

2 

4 

5 

6 

0 2 4 6 8 Min. 

7 

Standards Mix 

Ibuprofen (U.S.P.) 

1 

2 

10696 

1. Nicotine 

2. Quinine 


4. Nortriptyline 

5. 2,4, Dichlorophenoxy Acetic Acid 

6. 2,4, Dichlorophenoxy Propionic Acid 

7. 2,4, Dichlorophenoxy Butyric Acid 

Column: GraceSmart RP18, 5μm, 4.6 x 150mm 

Mobile Phase: 50% MeCN/50% 50mM, KH2PO4, pH 3 at 35°C 

10702 

1. Valerophenone 

2. Ibuprofen 


Hypersil ® 

BDS 

C18 

Kromasil ® 

100-5 

C18 

USP GraceSmart Luna Inertsil ® 

Spec C18 C18 ODS-3 

N/A 3.800 4.050 4.988 3.333 3.768 

Ret. Time 

(min) 

Theoretical ≥1700 8543 7216 6230 7049 7680 

plates 

Tailing ≤2.5 1.015 1.180 1.041 1.034 0.979 

Factor 

k' 1.1≤k'≤2.8 1.2 1.6 2.3 0.99 1.6 

0 

2.5 5 7.5 10 12.5 15 17.5 20 Min. 


(Part No. 5138812) 

Mobile Phase: H2O (adjusted with Phosphoric Acid to a pH of 2.5): 

Acetonitrile (65:35) 




1 

2 

4 

3 

Carbamates 

5 

6 

7 

0 2 4 6 8 10 12 14 16 18 20 Min. 

10739 

1. Fenuron 

2. Monuron 

3. Fluometuron 

4. Diuron 

5. Methiocarb 

6. Linuron 

7. Neburon 


(Part No. 5138812) 

Mobile Phase: A: 0.025M KH2PO4, pH 3.2 B: Acetonitrile 


%B: 30 60 








1 

2 

3 

4 

5 

6 

7 

0 2 4 6 8 10 12 14 Min. 

Column: 

10733 



3. Pyridoxamine 


5. Folic Acid 

6. Vitamin B12 

7. Riboflavin 

GraceSmart C18 Column, 5μm, 4.6 x 150mm 

(Part No. 5138812) 

Mobile Phase: A: 0.1M NH4H2PO4 Buffer, pH 4.5 

B: Acetonitrile 


%B: 5 5 40 40 

Flow Rate: 

Detector: 

Injection Vol: 

Water Soluble Vitamins 

1.0mL/min 

UV at 254nm 

2μL 




GraceAlpha Columns (Patent Pending) 


• High-porosity surface increases mass transfer and results in increased column 

efficiencies and loading capacity 

• Dense core and highly spherical shape yields mechanically robust particle 

• Proprietary bonding technology gives unique selectivity and increased resolution 

• Ideally suited for scale up to prep applications 

Extreme Porosity 

High-porosity exterior increases mass transfer leading to higher 

efficiencies and superior loading capacity. 

The high-porosity structure adds an elastic quality to the silica surface 

allowing the particles to pack tighter yielding higher efficiencies. It also 

stabilizes the packed bed preventing voids. 

Dense Inner Core 

new 

Dense core provides exceptional strength. Typically the high-porosity 

structure would result in a weak particle prone to breaking, but the 

GraceAlpha particle combines the high porosity outer region with a dense 

inner core that results in a particle that resists breaking and forming fines 

that contribute to high backpressures. 

GraceAlpha Phase Specifications 


C18 Silica Spherical 5, 10, 15, 20µm 120Å 325m 2 /g 15% Monomeric Yes L1 

C8 Silica Spherical 5, 10, 15, 20µm 120Å 325m 2 /g 10% Monomeric — L7 

Silica Silica Spherical 5, 10, 15, 20µm 120Å 325m 2 /g — Monomeric — L3 

High Level of Resolution 

GraceAlpha offers resolution advantages due to its higher 

mass transfer and optimized C18 and C8 bonding chemistry. 

Adsorbence 

Adsorbence 

1.0 

0.5 

0 

1.0 

0.5 

0 

0 

0 

Crude 20-AA Synthetic Peptide 

from Biopeptide Co., Inc. 

Better resolution 

of closely 

eluting impurities 

GraceAlpha Column 5µm 

5 

Competitor 4µm 

5 

10 15 19.99 Min. 

10 15 19.99 Min. 


Packing i.d. x Length Part No. 

C18, 5µm 4.6 x 150mm 5140743 

4.6 x 250mm 5140702 

C18, 10µm 4.6 x 150mm 5140744 

4.6 x 250mm 5140701 

C18, 15µm 4.6 x 150mm 5141058 

4.6 x 250mm 5141059 

C8, 5µm 4.6 x 150mm 5140740 

4.6 x 250mm 5140704 

C8, 10µm 4.6 x 150mm 5140742 

4.6 x 250mm 5140703 

C8, 15µm 4.6 x 150mm 5141055 

4.6 x 250mm 5141057 

Silica, 5µm 4.6 x 150mm 5140740 

4.6 x 250mm 5140704 

Silica, 10µm 4.6 x 150mm 5140742 

4.6 x 250mm 5140703 

Silica, 15µm 4.6 x 150mm 5141055 

4.6 x 250mm 5141057 

Columns: All C18 4.6 x 250mm 

Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile 


%B: 20 40 


The target peptide is better resolved from impurities, with 33% higher 

recovery, on the GraceAlpha column. 





52 



Alltech ® Brava Columns 

If You Like Hypersil ® BDS, Try This Lower-Cost Alternative 

• BDS phases for acids, bases, and neutrals 

• ODS phases for non-polars and lipophilics 

Medium-sized pores are ideal for resolution of small- to medium-sized compounds, 

while its less-retentive nature means less mobile phase consumption. 

Brava HPLC Columns 


Waters ® 

Fittings 

Part No. 

C18 BDS, 3µm 4.6 x 100mm 50910 50911 

4.6 x 150mm 50912 50913 

C18 BDS, 5µm 4.6 x 150mm 50914 50915 

4.6 x 250mm 50916 50917 

C18 ODS, 3µm 4.6 x 100mm 50957 50959 

4.6 x 150mm 50961 50963 

C18 ODS, 5µm 4.6 x 150mm 50965 50967 

4.6 x 250mm 50969 50971 

C8 BDS, 3µm 4.6 x 100mm 50918 50919 

4.6 x 150mm 50920 50921 

C8 BDS, 5µm 4.6 x 150mm 50922 50923 

4.6 x 250mm 50924 50925 

C8, 3µm 4.6 x 100mm 50939 50943 

4.6 x 150mm 50945 50947 

C8, 5µm 4.6 x 150mm 50949 50951 

4.6 x 250mm 50953 50955 

Phenyl, 5µm 4.6 x 150mm 50978 50979 

4.6 x 250mm 50981 50983 

Cyano BDS, 5µm 4.6 x 150mm 50926 50927 

4.6 x 250mm 50928 50929 

Cyano, 5µm 4.6 x 150mm 50973 50975 

4.6 x 250mm 50976 50977 

Amino, 5µm 4.6 x 150mm 50930 50931 

4.6 x 250mm 50933 50934 

Silica, 5µm 4.6 x 150mm 50935 50936 

4.6 x 250mm 50937 50938 

best 

value 

Brava Phase Specifications 



C18 ODS Silica Spherical 3, 5µm 130Å 195m 2 /g 8.5% Monomeric Yes L1 



Phenyl Silica Spherical 5µm 130Å 195m 2 /g — Monomeric No L11 

Cyano Silica Spherical 5µm 130Å 195m 2 /g — Monomeric No L10 

Cyano BDS Silica Spherical 5µm 145Å 185m 2 /g — Monomeric No L10 

Amino (NH2) Silica Spherical 5µm 130Å 195m 2 /g — Monomeric No L8 


2 

1 

0 2 

Metal Chelator 

4 Min. 

Column: Brava C18 BDS, 5µm, 4.6 x 150mm 

(Part No. 50914) 

Mobile Phase: Acetonitrile:50mM KH2PO4 pH 3 (50:50) 



10473 

1. Pyridine 

2. 2, 2’ Dipyridyl 


Brava All-Guard Cartridges* 


C18 BDS, 5µm 4.6 x 7.5mm 3 96472 

C18 ODS, 5µm 4.6 x 7.5mm 3 96477 

C8 BDS, 5µm 4.6 x 7.5mm 3 96473 

C8, 5µm 4.6 x 7.5mm 3 96478 

Phenyl, 5µm 4.6 x 7.5mm 3 96480 

Cyano BDS, 5µm 4.6 x 7.5mm 3 96474 

Cyano, 5µm 4.6 x 7.5mm 3 96479 

Amino, 5µm 4.6 x 7.5mm 3 96475 

Silica, 5µm 4.6 x 7.5mm 3 96476 


ea 80101 










Alltech ® Alltima Introduction 

High-Quality General-Purpose HPLC Columns 

• Extensive collection of published applications 

• Well suited for microbore applications, stable in popular microbore mobile phases 

• Acid and base deactivated symmetrical peaks 

• Polymerically bonded and double-endcapped for long column lifetimes 

• Analyze acids, bases, and neutrals in one run 

• Full range of column dimensions, from microbore to preparative 

Alltima Phase Specifications 



C18 LL Silica Spherical 5µm 100Å 340m 2 /g 9% Polymeric Yes L1 


Phenyl Silica Spherical 3, 5µm 100Å 340m 2 /g 7.5% Polymeric Yes L11 

Cyano Silica Spherical 3, 5µm 100Å 340m 2 /g — Polymeric Yes L10 


Silica Silica Spherical 3, 5, 10µm 100Å 340m 2 /g — — — L3 

1 

2 

3 

4 

Basic Drugs 

8193 

1. Procainamide 

2. Acetaminophen 

3. Theophylline 

4. Caffeine 

best 

seller 

1 

2 

Antibacterials 

3 

8764 

1. Oxytetracycline 

Dihydrate 

2. Tetracycline HCL 

3. Meclocycline 

Sulfosalicylate Salt 

0 5 10 15 Min. 

Column: Alltima C8, 5µm, 4.6 x 150mm 

Mobile Phase: Methanol:50mM KH2PO4, pH 3.0 (20:80) 



0 2 4 6 8 10 Min. 


Mobile Phase: A: 50mM KH2PO4, pH 3.0 B: CH3CN 


%B: 15 40 



Natural Products 

8887 

4 

Antihistamines 

7232 

1 

2 

3 

4 

1. Naringin 

2. Neohesperidin 

3. Myricetin 

4. Quercetin 

5. Naringenin 

6. Kaempferol 

5 6 

1 

2 

3 

5 

1. Tripelennamine 

2. Triprolidine 

3. Cyclizine 

4. Chlorcyclizine 

5. Meclizine 

0 5 

10 15 Min. 




%B: 20 40 



0 5 10 15 20 25 Min. 




%B: 25 25 70 



54 



Alltech ® Alltima Reversed-Phase Columns 

1 

0 1 

2 

7 

4,5 6 

3 

2 

Taxol ® and Related Taxanes in Yew 

(taxus brevifolia) 

3 

8 9 

1011 

12 13 

4 

5 6 

7 

1. 10-deacetylbaccatin III 

2. Baccatin III 

3. 7-xylosyl-10-deacetyltaxol B 

4. Taxinine M 

5. 7-xylosyl-10-deacetyltaxol 

6. 7-xylosyl-10-deacetyltaxol C 

7. 10-deacetyltaxol 

8. 7-xylosyltaxol 

9. Cephalomannine (Taxol ® B) 

10. 7-epi-10-deacetyltaxol 

11. Taxol ® 

12. Taxol ® C 

13. 7-epitaxol 

8 Min. 

Column: Alltima Phenyl Rocket, 3µm, 7 x 53mm 

Mobile Phase: 0.05% TFA in H2O:MeOH:CH3CN (50:19:31) 



123 

4 

Anticonvulsants 

0 4 8 12 16 20 Min. 

5 

Column: Alltima Phenyl, 5µm, 4.6 x 250mm 

Mobile Phase: A: H2O B: CH3CN 


%B: 15 60 60 15 



6 

9221 

7233 

1. Ethotoin 

2. Phenobarbital 

3. Methsuximide 

4. Mephenytoin 

5. Carbamazepine 

6. Diazepam 

Alltima All-Guard Cartridges* 


C18, 5µm 2.1 x 7.5mm 3 96680 

3.0 x 7.5mm 3 96361 

4.6 x 7.5mm 3 96080 

C18-LL, 5µm 2.1 x 7.5mm 3 96432 

3.0 x 7.5mm 3 96433 

4.6 x 7.5mm 3 96285 

C8, 5µm 2.1 x 7.5mm 3 96441 

3.0 x 7.5mm 3 96362 

4.6 x 7.5mm 3 96081 

Phenyl, 5µm 2.1 x 7.5mm 3 96442 

3.0 x 7.5mm 3 96445 

4.6 x 7.5mm 3 96082 


ea 80101 



Alltima HPLC Reversed-Phase Columns 

Standard 

Part No. 

Waters ® 

Fittings 

Part No. 


C18, 3µm Microbore 1.0 x 100mm 43853 — 

Microbore 1.0 x 150mm 43864 — 

Expedite MS 2.1 x 10mm 43836 — 








Analytical 4.6 x 50mm 81412 — 



Rocket 7 x 33mm 50603 — 

Rocket 7 x 53mm 50605 — 










C18-LL, 5µm Microbore 1.0 x 150mm 88390 — 








C8, 3µm Analytical 4.6 x 50mm 81413 — 



Rocket 7 x 33mm 50607 — 

Rocket 7 x 53mm 50609 — 









Phenyl, 3µm Microbore 1.0 x 100mm 43892 — 












Phenyl, 5µm Solvent Reducer 3.0 x 150mm 81147 — 






Alltech ® Alltima C18 Validation Kits 

Alltima columns have the batch-to-batch and column-tocolumn 

reproducibility required for method development. Each 

kit includes three columns made from three different batches 

of Alltima C18. 

Alltima is Stable and Reproducible 

Initial Separation 

7553 


Alltima C18 Validation Kits 

Description Includes: i.d. x Length Part No. 

C18, 3µm 3 columns from 3 different batches 4.6 x 100mm 11350 



Alltech ® Alltima 

Normal-Phase Columns 

Alltima HPLC Normal-Phase Columns 

Standard 

Part No. 

Waters ® 

Fittings 

Part No. 


Cyano, 3µm Analytical 4.6 x 50mm 81179 — 





Amino, 3µm Analytical 4.6 x 50mm 81180 — 





Silica, 3µm Microbore 1.0 x 100mm 43824 — 















Alltima All-Guard Cartridges* 


Cyano, 5µm 4.6 x 7.5mm 3 96084 

Amino, 5µm 4.6 x 7.5mm 3 96085 

Silica, 5µm 2.1 x 7.5mm 3 96450 

4.6 x 7.5mm 3 96083 


ea 80101 



1 

2 

4 

5 

0 5 10 15 20 25 30 35 40 45 50 55 Min. 

After 500 Hours of Washing at 60°C 

1 

2 

0 5 10 15 20 25 30 35 40 45 50 55 Min. 


Mobile Phase: A: Water:Acetonitrile:TFA (95:5:0.1) 

B: Water:Acetonitrile:TFA (25:75:0.1) 


% B: 5 30 



Column capacity and selectivity remained constant after washing an 

Alltima C18 column for 500 hours with 0.5% TFA:Acetonitrile (50:50) 

at 60°C. 

3 

3 

4 

5 

7554 

1. Gly-Tyr 

2. Val-Tyr-Val 

3. Met-Enkephalin 

4. Leu-Enkephalin 

5. Angiotensin II 

tech tip 





columns for low volume and microbore systems. See page 31 for 

more explanation of the Rocket and Expedite formats. 

56 



Alltech ® Apollo Columns 

Economical, High Performance Columns 

• Easy scale-up from analytical to prep 

• Extended pH stability—1.5 to 10.5 

Batch-tested Apollo HPLC columns use high purity, basedeactivated 

silica for powerful separations at an economical 

price. They are ideal for educational labs or for method 

development. 

Apollo Phase Specifications 





Silica Silica Spherical 5µm 100Å 340m 2 /g — — — L3 

Apollo HPLC Columns 






Phenyl, 5µm Analytical 4.6 x 150mm 36538 




best 

value 

Apollo All-Guard Cartridges* 


C18, 5µm 4.6 x 7.5mm 3 96454 

C8, 5µm 4.6 x 7.5mm 3 96463 

Phenyl, 5µm 4.6 x 7.5mm 3 96430 

Silica, 5µm 4.6 x 7.5mm 3 96419 


ea 80101 




Alltech ® Econosphere Columns 

• Low-cost columns for common chromatography applications 

• Acid-washed for low metal content 

• Easy scale-up from analytical to prep 

Econosphere Phase Specifications 





Amino (NH2) Silica Spherical 5µm 80Å 200m 2 /g — Polymeric Yes L8 


Econosphere HPLC Columns 

Standard 

Part No. 

Waters ® 

Part No. 





Rocket 7 x 33mm 50673 — 

Rocket 7 x 53mm 50675 — 










Econosphere HPLC Columns (continued) 

Standard 

Part No. 

Waters ® 

Part No. 









Econosphere All-Guard Cartridges* 


C18 4.6 x 7.5mm 3 96121 

C8 4.6 x 7.5mm 3 96122 

Cyano 4.6 x 7.5mm 3 96123 

Amino 4.6 x 7.5mm 3 96124 

Silica 4.6 x 7.5mm 3 96125 


ea 80101 


*Guard holder required. 



Alltech ® Adsorbosphere Columns 

• Reliable performance from rugged, reproducible phases 

High temperature bonding for exceptional surface coverage, stability, and reproducibility. 

Adsorbosphere columns are the workhorses of any chromatography lab. Choose from 

four varieties: 


Adsorbosphere has a moderate carbon load for common applications. 

Adsorbosphere HS has a larger surface area and higher carbon load for stronger retention of hydrophobic compounds. 

Adsorbosphere UHS has the largest surface area and highest carbon load for the highest resolution of hydrophobic 

compounds. 

Adsorbosphere XL is a lower-cost, larger pore alternative. C18-B separates strongly basic analytes more efficiently then 

standard C18. 

Adsorbosphere Phase Specifications 


Adsorbosphere C18 Silica Spherical 3, 5, 10µm 80Å 200m 2 /g 12% Monomeric Yes L1 

Adsorbosphere C18 HS Silica Spherical 3, 5µm 60Å 350m 2 /g 20% Monomeric Yes L1 

Adsorbosphere C18 UHS Silica Spherical 5, 10µm 60Å 500m 2 /g 30% Monomeric Yes L1 

Adsorbosphere C8 Silica Spherical 5µm 80Å 200m 2 /g 8% Monomeric Yes L7 

Adsorbosphere Phenyl Silica Spherical 5µm 80Å 200m 2 /g 5% Monomeric Yes L11 

Adsorbosphere Cyano Silica Spherical 5µm 80Å 200m 2 /g — Monomeric Yes L10 

Adsorbosphere Cyano-AQ Silica Spherical 5µm 120Å 170m 2 /g — Polymeric No L10 

Adsorbosphere Amino (NH2) Silica Spherical 3, 5µm 80Å 200m 2 /g — Polymeric No L8 

Adsorbosphere Silica Silica Spherical 5µm 80Å 200m 2 /g — — — L3 

Adsorbosphere SAX Silica Spherical 5µm 80Å 200m 2 /g — Monomeric — — 

Adsorbosphere SCX Silica Spherical 5µm 80Å 200m 2 /g — Monomeric Yes — 


Adsorbosphere XL C18-B Silica Spherical 5µm 90Å 200m 2 /g 12% Monomeric Yes L1 


Adsorbosphere XL C1 (TMS) Silica Spherical 5µm 90Å 200m 2 /g — Monomeric Yes L13 

Adsorbosphere XL Silica Silica Spherical 5µm 90Å 200m 2 /g — — — L3 

Adsorbosphere XL SAX Silica Spherical 5, 10µm 90Å 200m 2 /g — Monomeric Yes — 

Adsorbosphere XL SCX Silica Spherical 5, 10µm 90Å 200m 2 /g — Monomeric Yes — 

0 10 20 30 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Column Temp: 

Vitamins D2 & D3 

1 

2 

5401 

1. Vitamin D2 

2. Vitamin D3 

Adsorbosphere UHS C18, 5µm, 4.6 x 150mm 

(Part No. 288118) 

80% Acetonitrile 20% Methanol 

1.5mL/min 

UV at 254nm 

Ambient 

Adsorbosphere HPLC Columns 

Standard 

Part No. 

Waters ® 

Fittings 

Part No. 



Rocket 7 x 33mm 50623 — 

Rocket 7 x 53mm 50625 — 









Cyano-AQ, 5µm Analytical 4.6 x 150mm 50005 50035 






SCX, 5µm Analytical 4.6 x 250mm 287472 287473 

Adsorbosphere HS HPLC Columns 

Standard 

Part No. 

Waters ® 

Fittings 

Part No. 


C18 HS, 3µm Analytical 4.6 x 100mm 28780 28781 


C18 HS, 5µm Analytical 4.6 x 150mm 28817 28818 


58 



Alltech ® Adsorbosphere 

Columns (continued) 

Adsorbosphere UHS HPLC Columns 

Adsorbosphere XL HPLC Columns 

Standard 

Part No. 

Standard 

Part No. 

Waters ® 

Fittings 

Part No. 


C18 UHS, 5µm Analytical 4.6 x 150mm 288118 288119 

C18 UHS, 10µm Analytical 4.6 x 250mm 288133 288134 

Waters ® 

Fittings 

Part No. 


XL C18, 3µm Analytical 4.6 x 100mm 20237 20286 


Rocket 7 x 33mm 50633 — 

Rocket 7 x 53mm 50635 — 



XL C18-B, 5µm Analytical 4.6 x 150mm 20548 20344 







XL Silica, 5µm Analytical 4.6 x 150mm 20365 20398 


XL SAX, 5µm Analytical 4.6 x 150mm 20538 20318 


XL SAX, 10µm Analytical 4.6 x 250mm 20314 20303 

XL SCX, 5µm Analytical 4.6 x 150mm 20525 20531 


XL SCX, 10µm Analytical 4.6 x 250mm 20719 20684 

Alltech ® Allsphere Columns 

Try Alltech’s lower cost Allsphere columns as a replacement 

for Spherisorb ® columns. If you are not satisfied with 

Allsphere as a direct replacement for your Spherisorb ® 

applications, we will refund your money. 

Allsphere HPLC Columns 

Standard 

Part No. 

Waters ® 

Fittings 

Part No. 

Packing 

i.d. x Length 

ODS-1, 5µm 4.6 x 150mm 778441 778443 

4.6 x 250mm 778364 778357 

ODS-2, 3µm 4.6 x 100mm 778487 778489 

4.6 x 150mm 778558 778560 

ODS-2, 5µm 4.6 x 150mm 778545 778547 

4.6 x 250mm 778736 778738 

C8, 3µm 4.6 x 100mm 778561 778563 

4.6 x 150mm 778609 778611 

C8, 5µm 4.6 x 150mm 778571 778573 

4.6 x 250mm 778744 778746 

C6, 5µm 4.6 x 150mm 778211 778217 

4.6 x 250mm 778223 778224 

Phenyl, 5µm 4.6 x 150mm 778689 778691 

4.6 x 250mm 778752 778754 

Cyano, 5µm 4.6 x 150mm 778713 778715 

4.6 x 250mm 778361 778362 

Amino, 5µm 4.6 x 150mm 778739 778741 

4.6 x 250mm 778371 778372 

Silica, 3µm 4.6 x 100mm 778382 778383 

4.6 x 150mm 778554 778556 

Silica, 5µm 4.6 x 150mm 778389 778391 

4.6 x 250mm 778376 778377 

SAX, 5µm 4.6 x 250mm 778765 778767 

SCX, 5µm 4.6 x 250mm 778188 778189 


Adsorbosphere All-Guard Cartridges* 

Packing i.d.x Length Qty. Part No. 

Amino, 5µm 4.6 x 7.5mm 3 96044 

C18, 5µm 4.6 x 7.5mm 3 96041 

C18 HS, 5µm 4.6 x 7.5mm 3 96079 

C18 UHS, 5µm 4.6 x 7.5mm 3 82181 

C8, 5µm 4.6 x 7.5mm 3 96042 

Cyano, 5µm 4.6 x 7.5mm 3 96047 

Cyano-AQ, 5µm 4.6 x 7.5mm 3 96048 

Phenyl, 5µm 4.6 x 7.5mm 3 96045 

Silica, 5µm 4.6 x 7.5mm 3 96046 

SAX, 5µm 4.6 x 7.5mm 3 96049 

SCX, 5µm 4.6 x 7.5mm 3 96075 

XL C18, 5µm 4.6 x 7.5mm 3 96088 

XL C18-B, 5µm 4.6 x 7.5mm 3 96089 

XL C8, 5µm 4.6 x 7.5mm 3 96090 

XL C1, 5µm 4.6 x 7.5mm 3 96093 

XL Silica, 5µm 4.6 x 7.5mm 3 96097 

XL SAX, 5µm 4.6 x 7.5mm 3 96098 

XL SCX, 5µm 4.6 x 7.5mm 3 96099 


ea 80101 



Allsphere All-Guard Cartridges* 


ODS-1, 5µm 4.6 x 7.5mm 3 96402 

ODS-2, 5µm 4.6 x 7.5mm 3 96403 

C8, 5µm 4.6 x 7.5mm 3 96404 

C6, 5µm 4.6 x 7.5mm 3 96405 

Phenyl, 5µm 4.6 x 7.5mm 3 96406 

Cyano, 5µm 4.6 x 7.5mm 3 96408 

Amino, 5µm 4.6 x 7.5mm 3 96409 

Silica, 5µm 4.6 x 7.5mm 3 96401 

SAX, 5µm 4.6 x 7.5mm 3 96410 

SCX, 5µm 4.6 x 7.5mm 3 96411 


ea 80101 



more info 

Looking for Adsorbosil ® columns? 

Please visit www.discoverysciences.com. 




Grom Sil Columns 

High-Quality, Wide Variety, HPLC Columns for All Types of Applications 

• Highly consistent manufacturing for excellent reproducibility 

• 40 different phases to choose from, general purpose to specialty phases 

• Wide range of particle sizes from sub 2μm to 10µm 

• Available in nano to preparative column formats 

High purity spherical silica with high and universal selectivity for broad range of applications. Different phases and bonding 

technologies give you the power and flexibility 

ST = standard bonding with endcapping—high-quality universal column 

NE = not endcapped—high polar selectivity 

HE = hydrophilic endcapping—increased polar selectivity; pH-stable at high pH, high carbon load and very low silanol activity 

CP = possiblility to work at high pH 

FE = Fully endcapped—maximum silica coverage 

PA = Cross-linked polyamino—better stability 

pH = pH stable—use mobile phases up to pH 11 

Grom Sil Phase Specifications 

Base Particle 

Material Shape Particle Size Pore Size Surface Area Carbon Load Phase Type 

Endcapped? 

Phase 

ODS-3 CP (encapsulated) Silica Spherical 3, 5, 7, 10µm 120, 300Å 320, 170m 2 /g 15, 6% Polymeric No L1 

ODS-4 HE (hydrophilic endcapping) Silica Spherical 3, 4, 5, 7, 10µm 120, 200Å 300, 200m 2 /g 16, 11% Monomeric Yes L1 

ODS-5 ST (standard) Silica Spherical 3, 4, 5, 7, 10µm 60, 120, 200Å 580, 300, 200m 2 /g 22, 17, 12% Monomeric Yes L1 

ODS-7 pH (pH-stable) Silica Irregular 4µm 80Å 510m 2 /g 22% Polymeric No L1 

Octyl-5 CP (encapsulated) Silica Spherical 3, 5, 7, 10µm 120, 300Å 320, 170m 2 /g 10, 5.5% Polymeric No L7 

Phenyl-1 FE (fully endcapped) Silica Spherical 3, 5, 10µm 120, 300Å 300, 150m 2 /g 9, 5% Monomeric Yes L11 

Phenyl-2 CP (encapsulated) Silica Irregular 5µm 120, 300Å 320, 170m 2 /g 7, 4% Polymeric No L11 

Cyano-3 CP (encapsulated) Silica Spherical 5µm 120Å 320m 2 /g 4% Polymeric No — 

Amino-2 PA (Crs linked Poly-NH) Silica Spherical 5µm 120Å 300m 2 /g — Polymeric No L8 

Diol Silica Spherical 5, 10µm 60, 120, 200Å 580, 300, 200m 2 /g — Monomeric No L20 

USP 

L-code 

0 

Color Additives—Dyes 

1 2 8 

3 

4 

5 

6 7 

9 10 

11 

10 20 30 40 Min. 

1. Tartrazine 

2. Amaranth 

3. Indigo Carmine 

4. New Coccine 

5. Sunset Yellow 

6. Fast Green 

7. Brilliant Blue 

8. Erythosine 

9. Acid Red 

10. Phloxine 

11. Rosé Bengale 

Column: Grom Sil 120 ODS-5 ST, 5µm, 4 x 150mm 


Mobile Phase: A: 10mM NH4-phosphate, pH 6.0:MeOH (90:10) 

B: 10mM NH4-phosphate, pH 6.0:MeOH (20:30) 

Gradient: 0–100% B (0–40min, linear), 100% B (40–60min) 



0 

1 

4 5+6 

2 3 7+8 

9 

1011 12 13 14 

17 

15 18 

16 

5 10 15 20 25 30 35 Min. 

Nucleosides and Bases 

1. Cytosine 

2. Cytidine 

3. Uracil 

4. Deoxycytidine 

5. Guanine 

6. Adenine 

7. Hypoxanthine 

8. Uridine 

9. Xanthine 

10. Thymine 

11. Inosine 

12. Guanosine 

13. Deoxyinosine 

14. Deoxyguanosine 

15. Xanthosine 

16. Thymidine 

17. Adenosine 

18. Deoxyadenosine 

Column: Grom Sil 120 ODS-5 ST, 5μm, 4 x 250mm 


Mobile Phase: A: 20mM NH4-acetate, pH 3.5 

B: 20mM NH4-acetate, pH 3.5:MeOH (90:10) 


%B: 30 30 100 100 

Detector: UV at 260nm, 0.08 AUFS 

60 




0 

1 

2 

Water-soluble Vitamins 

3 

4 

5 

6 


(Part No. 5113642) 


Mobile Phase: A: 20mM K-phosphate, pH 6.6 

B: 20mM K-phosphate, pH 6.6:ACN (80:20) 


%B: 0 0 100 100 



0 

1 

5 10 15 20 25 Min. 

2 3 4 

6 7 

5 

8 

Fat-soluble Vitamins 

9 

1. Menadione (Vit. K3) 

2. Retinol (Vit. A) 

3. Retinol Acetate 

4. Menaquinone (Vit. K2) 

5. d-Tocopherol 

6. Ergocalciferol (Vit. D2) 

7. Cholecalciferol (Vit. D3), 

8. a-Tocopherol (Vit. E), 

9. a-Tocopherol Acetate, 

10. Phylloquinone (Vit. K1) 


(Part No. 5113642) 


Mobile Phase: Acetonitrile 



10 

5 10 15 20 25 Min. 

7 

8 

1. L-Ascorbic Acid (Vit. C) 


3. Pyridoxine HCl (Vit. B6) 

4. Nicotinamide 

5. Thiamine Hydrochloride 

(Vit. B1) 

6. Folic Acid 

7. Cyanocobalamin 

(Vit. B12) 

8. Riboflavin 



ODS-3 CP, 3µm Microbore 1 x 60mm 5115722 

Microbore 2 x 125mm 5115672 


Solvent Reducer 3 x 60mm 5113597 



Analytical 4 x 60mm 5113598 


ODS-3 CP, 5µm Solvent Reducer 3 x 125mm 5113604 



ODS-4 HE, 10µm Analytical 4.6 x 250mm 5116144 

ODS-4 HE, 3µm Capillary 0.3 x 150mm 5118148 










ODS-4 HE, 5µm Capillary 0.1 x 150mm 5117667 









ODS-5 ST, 3µm Microbore 2 x 60 mm 5117044 


ODS-5 ST, 5µm Microbore 2 x 250mm 5116342 





ODS-7 pH, 4µm Capillary 0.3 x 250mm + 5119756 

10mm Guard Column 






Octyl-5 HE, 3µm Analytical 4.6 x 125mm 5113265 

Octyl-5 CP, 5µm Microbore 2 x 250mm 5117325 


Phenyl-1 FE, 5µm Microbore 2 x 100mm 5113695 


Phenyl-2 CP, 5µm Analytical 4 x 250mm 5116429 

Cyano-3 CP, 5µm Microbore 2 x 125mm 5117410 


Amino-2 PA, 5µm Microbore 2 x 250mm 5115469 



Diol, 5µm Microbore 2 x 100mm 5117476 






more info 

For large molecule columns, refer to page 84–97. 




Grom Sapphire Column 

High Purity for Optimal Performance of Challenging Separations 

• Excellent peak shape—Highly symmetrical peaks from high purity silica base 

• High speed and high capacity—Range of pore sizes and surface area for improved speed and resolution 

• Optimized for consistent flow—critical in low volume applications 

• Variety of column formats—1.5, 3, and 5µm particles 

Sapphire Phase Specifications 




C4* Silica Spherical 3, 5, 10µm 65, 110Å 500, 270m 2 /g 10.5, 7% Monomeric Yes L26 

Silica* Silica Spherical 3, 5, 10µm 65, 110Å 500, 270m 2 /g — — No L3 

*Columns available upon request. 

1 

4 

2 

3 

5 

Separation of Antiepileptics 

6 

7 8 

1. Ethosuximide 

2. Primidone 

3. Lamotrigine 


5. Carbamazepine-10,11-epoxide 

6. IS, Hexobarbital 

7. Phenytoin 


Stability of Grom Sapphire Packings (Daldrup) 

1000 mV 

500 

2 

1 3 

4 

5 

1. Uracil 

2. 4-Hydroxybenzoic Acid 

3. Diphenylhydramine 

4. 5-(p-Methylphenyl)-5-phenylhydantoin 

5. Diazepam 

6. Toluene 

6 

1000 Injection 

500 Injection 

0 1 2 3 4 Min. 

Column: Grom Sapphire 110 C18, 5µm, 4 x 53mm 

(Part No. 5117499) 


Mobile Phase: 50mM Na-phosphate, pH 6.5:MeOH:ACN (62:23:15) 


Sapphire HPLC Columns 


C18, 3µm 0.030 x 150mm 5119303 

0.075 x 150mm 5133239 

0.100 x 100mm 5135280 

C18, 5µm 4 x 53mm 5117499 

4 x 125mm 5117505 

C8, 5µm 2 x 250mm 5118934 

0 

1 Injection 

4 8 12 16 20 24 28 32 36 Min. 

Column: Grom Sapphire 110 C18, 5µm, 4 x 125mm 

(Part No. 5117505) 


Mobile Phase: 50mM Na-phosphate, pH 2.3:ACN (58:42) 

Column Temp: RT 







Looking for HPLC column 

prefilters? See page 111. 


Need high-pressure 

polymeric fittings? 

See pages 112–114 

for our full selection of 

high-pressure fittings. 

6673 

62 



Grom Amino Acid Column Kits 

Amino Acid Analysis by Precolumn 

Derivatization Using OPA/IBLC 

• High resolution (both optical antipodes of all protein 

amino acids in a single chromatogram) 

• Outstandingly high sensitivity (fMol) 

• Extraordinarily long lifetimes of columns 

1. L-Asp 

2. D-Asp 

3. L-Glu 

4. D-GLu 

5. L-Ser 

6. D-Ser 

7. L-Thr 

OPA/IBLC Derivatized D- and L-Amino Acids 

12 34 

14 

2930 

12 15 

5 6 22 

7 

25 

8 

19 21 

13 

18 20 

910 26 

23 28 

24 27 

11 16 17 

0 10 20 30 40 50 60 70 Min. 

8. Gly 

9. D-Thr 

10. L-His 

11. D-His 

12. L-Ala 

13. L-Arg 

14. D-Arg 

15. D-Ala 

16. L-Tyr 

17. D-Tyr 

18. L-Val 

19. L-Met 

20. L-Nor-Val 

(Int. Standard) 

21. D-Met 

22. D-Val 

23. L-Phe 

24. L-Ile 

25. D-Phe 

26. L-Leu 

Column: Grom Sil OPA-2, 4 x 250mm 


Mobile Phase: A: 23mM Na-acetate, pH 6.0 

B: 600mL MeOH + 50mL ACN 

Gradient: 0–53% B (0–75min) 


Detector: Fluorescence 230nm(exc.), 445nm(em.) 

27. D-Ile 

28. D-Leu 

29. L-Lys 

30. D-Lys 


Derivatization With OPA/3-MPA 

• Highest sensitivity (5–10fMol) 

• Short analysis time 

• Fully automated derivatization 

• Best resolution 

Amino Acid Analysis after Precolumn Derivatization 

with OPA/Mercaptopropionic Acid — Extended Standard 

1. Asp 

2. Glu 

3. Asn 

4. Ser 

1 

2 

16 17 

9 15 

13 

20 21 22 

4 6 

11 

7 12 18 

14 

3 8 10 

19 

5 

24 

23 

0 10 20 30 40 Min. 

5. Gin 

6. Gly 

7. Thr 

8. His 

9. Cit 

10. m-His 

11. Ala 

12. Tau 

13. Arg 

14. AAB 

15. Tyr 

16. Val 

17. Met 

18. Nor-Val 

19. Trp 

20. Ile 

21. Phe 

22. Leu 

23. Orn 

24. Lys 

Column: Grom Sil OPA-1, 4 x 250mm 

Mobile Phase: A: 25mM Na-phosphate, pH 7.2:THF (995:5) 

B: 25mM Na-phosphate, pH 7.2:MeOH/ACN (50:35:15) 

Gradient: Time: 0 3 20 25 36 40 43 

%B: 0 0 35 50 60 70 100 





OPA/IBLC Kit 

Description 

Complete kit includes 4.0mm i.d. x 250mm length column, 

OPA/IBLC derivitization reagents, and detailed working 

instructions. 

Part No. 

987.0000 

OPA/3-MPA Kit 

Description 


OPA/3-MPA derivitization reagents, and detailed working 

instructions. 

Part No. 

980.0000 


Derivatization Using FLEC/ADAM 

• High resolution and high sensitivity 

• Rapid, automated or manual derivatization 

• Primary and secondary amines 

• Stable derivatives 

FLEC/ADAM Kit 

Description 


FLEC/ADAM derivitization reagents, and detailed working 

instructions. 

Part No. 

984.0000 

Separation of a D-, L-Amino Acid Standard by HPLC 

Employing the FLEC-ADAM Approach 

1. L-Arg 

2. D-Arg 

3. L-Ser 

4. D-Ser 

5. D-Asp 

6. L-Asp 

7. D-Thr 

8. L-Thr 

9. D-Glu 

10. L-Glu 

11. D,L-Pro 

12. D-Ala 

13. L-Ala 

14. FLEC-OH 

15. D-Tyr 

16. D-Met 

17. L-Tyr 

18. L-Met 

19. D-Val 

20. D-Phe 

11 

14 

26 

12 20 22 29 

13 

2 34 7 

18 

8 9 16 19 21 

25 

1 10 

5 

6 

1517 

23 

24 27 28 

31 3233 

30 

20 30 40 50 60 70 Min. 

21. L-Val 

22. L-Phe 

23. D-allo-lie 

24. D-lle 

25. D-Leu 

26. L-allo-lie; 

L-lie 

27. L-Leu 

Column: 

Flow Rate: 

Mobile Phase: 


%B: 17 17 30 50 

%C: 8 8 0 0 

28. D-His 

29. D-Om 

30. L-His 

31. D-Lys 

Grom Sil FLEC-1, 4 x 250mm 

0.75mL/min 

A: 50mM Na-acetate, pH 4.0 B: ACN C: THF 



32. L-Om 

33. L-Lys 




Jones Genesis ® Columns 

Genesis ® columns use a new generation adsorbent based 

on high-purity metal-free 120Å spherical silica. Particle size 

offering includes 3, 4, 7, and 15µm. Genesis ® columns exhibit 

excellent peak symmetry and exceptional pH stability from 

1 to 10. 

Genesis ® Phase Specifications 


C18 Silica Spherical 3, 4, 7, 15µm 120Å 300m 2 /g 18% Monomeric Yes L1 

AQ Silica Spherical 4, 7µm 120Å 300m 2 /g 15% Monomeric Yes L1 


C8e/c Silica Spherical 3, 4, 7, 15µm 120Å 300m 2 /g 11% Monomeric Yes L7 

C4 Silica Spherical 4µm 120Å 300m 2 /g 6.3% Monomeric Yes L26 

Phe Silica Spherical 4µm 120Å 300m 2 /g 9.4% Monomeric Yes L11 

CN Silica Spherical 3, 4µm 120Å 300m 2 /g 7% Monomeric Yes L10 

Amino (NH2) Silica Spherical 3, 4µm 120Å 300m 2 /g 3.5% Polymeric No L8 

Carbohydrate Silica Spherical 4µm 120Å 300m 2 /g — Monomeric — — 

CN-TCA Silica Spherical 4µm 120Å 300m 2 /g 7% Monomeric Yes — 

Petro-XP Silica Spherical 4µm 120Å 300m 2 /g — Monomeric — — 

Silica Silica Spherical 3, 4, 7, 15µm 120Å 300m 2 /g n/a n/a n/a L3 

Genesis ® C18 Reversed Phase 

• Excellent peak symmetry 

• Exceptional stability from pH 1 to 10 

• Reduced need for mobile-phase modifiers 

• Long column life 

Genesis ® C18 bonding is monomeric. A unique proprietary 

end-capping reagent, which is less prone to acid hydrolysis 

than trimethylsilane, provides freedom from residual silanols 

and enhanced stability under low-pH operating conditions. 

Genesis ® C18 columns also exhibit superior stability at 

alkaline pH. The permissible operating range is pH 1–10. 

Genesis ® AQ Reversed Phase 

• Designed for separating hydrophilic and polar 

compounds 

• Stable retention times in 100% aqueous mobile phases 

• Rapid equilibration 

• Unique reversed-phase selectivity 

The Genesis ® AQ adsorbent employs an optimum ratio of 

C18, short (non-TMS) chains, and polar surface groups 

bonded to high-purity 120Å silica to allow rapid equilibration 

and provide consistent, reproducible chromatography with 

stable retention times in 100% aqueous eluents. 

1 

2 

0 1 2 3 4 5 Min. 

Column: 

Flow Rate: 

Mobile Phase: 

Detector: 

Inertness Test 

3 

4 

1. Uracil 

2. Pyridine 

3. 2,3-Dimethylphenol 

4. Methylbenzoate 

Genesis ® FM15960E C18, 4µm, 4.6 x 150mm 

0.8mL/min 

Isocratic, 50% ACN in water 

UV at 254nm 

Retention on Genesis ® AQ is greater for polar analytes but 

lower for non-polar compounds compared to Genesis ® C18. 

Uracil, which is typically an unretained peak on C18 columns, 

is well retained on the Genesis ® AQ adsorbent. Acids and 

bases exhibit good peak shapes. Although Genesis ® AQ 

excels with water-rich mobile phases, it can also be used in 

gradient and isocratic modes with a full spectrum of mobile 

phases. 

1 

2 

3 4 

Nucleotide 5'-Monophosphates 

0 2 4 6 8 10 12 14 Min. 

5 

1. KNO3 

2. CMP 

3. UMP 

4. GMP 

5. AMP 

Column: Genesis ® FM15951E AQ, 4µm, 4.6 x 150mm 


Mobile Phase: Isocratic, 50mM NH4OAc, pH 5.5 



64 



Jones Genesis ® Columns 

Genesis ® C8(EC) Reversed Phase 

• Excellent peak symmetry 

• Exceptional stability from pH 1 to 10 

• Reduced need for mobile-phase modifiers 

• Long column life 

C8(EC) is double-bonded before fully endcapped using a 

unique proprietary end-capping reagent. It is less prone to 

acid hydrolysis than trimethylsilane, which provides freedom 

from residual silanols and enhanced stability under low-pH 

operating conditions. The bonding is monomeric. Genesis ® 

C8(EC) columns exhibit superior stability at alkaline pH. 

Genesis ® C8 Reversed Phase 

• Non-endcapped 

• Suitable for lower pH separations 

Genesis ® C8 bonding is monomeric. They are suitable 

for separations under lower pH conditions and may offer 

selectivity advantages for some samples. 

Genesis ® Phenyl 

• Unique reversed-phase chemistry 

• Improve the chromatography of polar aromatic, fatty 

acids, and basic pharmaceuticals 

Genesis ® Phenyl columns provide impressive peak symmetry 

for both acidic and basic compounds. 

Genesis ® Columns Genesis ® Guards 1 

Length: 30mm 50mm 100mm 150mm 200mm 250mm 10mm 20mm 

Genesis ® AQ 

4µm 1.0mm i.d. — FJ5951E FJ10951E FJ15951E — FJ25951E FJ1951-2 — 

2.1mm i.d. FK3951E FK5951E FK10951E FK15951E FK20951E FK25951E FK1951-2 FK2951-2 

3.0mm i.d. FL3951E FL5951E FL10951E FL15951E — FL25951E FL1951-2 FL2951-2 

4.0mm i.d. FH3951E FH5951E FH10951E FH15951E — FH25951E FH1951-2 FH2951-2 

4.6mm i.d. FM3951E FM5951E FM10951E FM15951E FM20951E FM25951E — — 

Genesis ® C18 



3.0mm i.d. FL3963E FL5963E FL10963E FL15963E — FL25963E — — 








Genesis ® C8 


2.1mm i.d. FK3968E FK5968E FK10968E FK15968E FL20968E FK25968E FK1968-2 FK2968-2 








Genesis ® C8(EC) 











Genesis ® Phenyl 




4.6mm i.d. FM3980E FM5980E FM10980E FM15980E FM20980E FM25980E FH1980-2 FH2980-2 

NOTE: Genesis ® line is completed by additional phases such as Silica, Phenyl, Cyano, and Amino, for details please check online at www.discoverysciences.com. 

1 

All Genesis ® guards listed are cartridges and require either a stand-alone holder or direct-connect holder for use. Guard cartridges are 2/pk. 


Guard Cartridge Holders for Genesis ® and Apex Guards 

10 mm Stand-Alone 20 mm Stand-Alone 10 mm Direct-Connect 20 mm Direct-Connect 

1.0 mm i.d. F91GPH — — — 

2.1 mm i.d. F9111P F9112P F9141P — 

3.0, 4.0, 4.6 mm i.d. F9111P F9112P F9151P F9152P 




Jones Apex Reversed-Phase Columns 

Economical Column Line with Maximum Reproducibility and Efficiency 

• Conventional 100Å pore size spherical silica 

• Uniform-sized particle 

• Narrow particle distribution 

• Controlled surface area 

Apex Phase Specifications 


ODS Silica Spherical 3, 5, 10µm 100Å 170m 2 /g 10% Polymeric Yes L1 

ODS II Silica Spherical 3, 5µm 100Å 170m 2 /g 10.5% Monomeric Yes L1 




Diol Silica Spherical 3, 5µm 100Å 170m2/g 3.5% Monomeric No 

Phe Silica Spherical 3, 5µm 100Å 170m 2 /g 3% Monomeric Yes L11 

CN Silica Spherical 3, 5µm 100Å 170m 2 /g 4% Monomeric No L10 


Amino II (NH2) Silica Spherical 3, 5µm 100Å 170m 2 /g 2% Monomeric No L8 

Carbohydrate Silica Spherical 5µm 100Å 170m 2 /g — Monomeric Proprietary — 

Silica Silica Spherical 3, 5, 10µm 100Å 170m 2 /g n/a% n/a No L3 

Apex HPLC Columns 


ODS, 3µm Analytical 4.6 x 50mm 5110394 

ODS, 5µm Analytical 4.6 x 30mm 5109740 




ODS II, 3µm Analytical 4.6 x 100mm 5110994 


ODS II, 5µm Analytical 4.6 x 50mm 5109944 







C8(EC), 5µm Analytical 4.6 x 30mm 5112354 


Diol, 5µm Analytical 4.6 x 150mm 5120721 

Phenyl, 5µm Analytical 4.6 x 100mm 5110997 



Cyano, 5µm Analytical 4.6 x 250mm 5109737 

Cyano II, 5µm Analytical 4.6 x 250mm 5109933 



Amino II, 3µm Analytical 4.6 x 150mm 5111004 




Apex Guard Columns 

Packings i.d. x Length Part No. 

ODS, 5µm 4 x 10mm 5110703 

ODS II, 5µm 4 x 20mm 5110705 

C8, 5µm 4 x 20mm 5112454 

C8-EC, 5µm 4 x 10mm 5112425 

Cyano, 5µm 4 x 10mm 5111079 

Amino, 5µm 4 x 10mm 5111078 

4 x 20mm 5112455 

Guard Cartridge Holder 10mm F9111P 

20mm 

F9112P 

Column: 

Flow Rate: 

Mobile Phase: 

2 4 

1 3 

Apex ODS II, C18, 5µm, 4.6 x 250mm 

0.8mL/min 

A: 10 mM NaOAc, 0.02% THF 

B: 10 mM NaOAc, 2.7% THF, 25% ACN 


%B: 0 70 



5 

6 

7 

Xanthines in Plasma 

11 

9 

8 

10 

0 4 8 12 16 20 24 28 Min. 

12 





1. 7-Methylxanthine 



4. 1,3-Methyluric Acid 

5. Theobromine 

6. Ethylxanthine 

7. Methyluric Acid 

8. Paraxanthine 


10. 1,3,7-Methyluric Acid 

11. Ethyltheophylline 

12. Caffeine 

66 



Alltech ® OPA Amino Acid 

Columns 

• OPA-HS for high-speed with protein hydrolysates 

• OPA-HR for high-resolution with physiological samples 

OPA-HS (high-speed) columns are ideal for the study of 

protein acid hydrolysates with very simple mobile-phase 

systems. 

OPA-HR (high-resolution) columns are ideal for applications 

that require more resolution than simple hydrolysates. 

OPA-HR gives baseline separation of most amino acids 

found in physiological fluids. 

0 

1 

2 

OPA-Derivatized Amino Acids 

3 

4 

5 6 

7 

8 

9 

OPA Amino Acid HPLC Columns 


OPA-HS, 5µm Analytical 4.6 x 100mm 28064 

OPA-HR, 5µm Analytical 4.6 x 150mm 28072 

Derivatization Reagents 

Description Qty. Part No. 

OPA Crystals 5g 35606 

10 

13 

11 

5 10 15 20 25 30 Min. 

12 

14 

15 

9234 

1. Aspartic Acid 

2. Glutamic Acid 

3. Serine 

4. Histidine 

5. Glycine 

6. Threonine 

7. Arginine 

8. Alanine 

9. Tyrosine 

10. Methionine 

11. Valine 

12. Phenylalanine 

13. Isoleucine 

14. Leucine 

15. Lysine 

Column: OPA-HR, 5µm, 4.6 x 150mm (Part No. 28072) 

Mobile Phase: A: Methanol B: Tetrahydrofuran 

C: 20mM Sodium Acetate buffer, pH 5.5 


%A: 5 65 

%B: 5 2 

%C: 90 23 


Detector: Fluorescence 

Alltech ® Catecholamine 

Columns 

• For routine analysis of indoles and catecholamines 

• Reliable and inexpensive 

With this column, easily resolve catecholamines under isocratic 

conditions at room temperature. A simple monochloroacetic 

acid mobile phase with varying amounts of sodium octyl 

sulfate and disodium EDTA controls the elution profile. 

Catecholamine HPLC Columns 


Catecholamine, 3µm Analytical 4.6 x 100mm 28916 

All-Guard Cartridges* 


Catecholamine, 3µm 4.6 x 7.5mm 3 96076 


ea 80101 



Alltech ® Nucleotide- 

Nucleoside Columns 

• Determine both nucleotides and nucleosides with a 

single column 

With this column, separate seven common nucleosides or 

twelve common nucleotides using a buffer/methanol/tetrabutyl 

ammonium phosphate gradient. 

Nucleotide-Nucleoside HPLC Columns 


Nucleotide-Nucleoside, 7µm Analytical 4.6 x 250mm 35623 

All-Guard Cartridges* 


Nucleotide-Nucleoside, 7µm 4.6 x 7.5mm 3 96077 


ea 80101 



7110 


more info 

For related products, see our Vydac ® line of Life Science columns 

on pages 85–95. 




Alltech ® Mixed-Mode 

Columns 

• Separate hydrophobic and ionic species on a 

single column 

• Control ionization with buffer adjustments instead of 

ion-pair reagents 

1 

1 

Anesthetics and Neutral 

2 

2 

3 

3 

8780 

8781 

1. Biphenyl 

2. Lidocaine 

3. Procaine 

Alltech ® Carbohydrate 

Cation Columns 

• High efficiency separations 

using only water as the 

mobile phase 

• Sulfonated polystyrene 

resin in the calcium form 

• Column heating required 

100% Pure Red Delicious Apple Juice 

(a 1:250 Dilution) 

4 

8965 

1. Unknown 

2. Sucrose 

3. Glucose 

4. Fructose 

5. Sorbitol 

7110 

2 

3 

0 5 

10 15 20 Min. 

Column: Mixed-Mode C8/Cation, 5µm, 4.6 x 150mm 

Mobile Phase: 8780: 55mM K2HPO4, pH6.5: Acetonitrile (50:50) 

8781: 45mM K2HPO4, pH6.5: Acetonitrile (50:50) 




Changing the buffer concentration affects the capacity of the cationic 

components lidocaine and procaine, but does not affect the capacity 

of biphenyl, a neutral component. 

Mixed-Mode HPLC Columns 


Waters ® 

Fittings 

Part No. 

C8/Anion, 7µm Analytical 4.6 x 150mm 71354 71355 


C8/Cation, 5µm Analytical 4.6 x 150mm 72568 72569 


C18/Cation, 5µm Analytical 4.6 x 150mm 72574 72575 


1 

0 5 

Carbohydrate Cation HPLC Columns 


Carbohydrate Cation, 10µm Analytical 6.5 x 300mm 70057 

more info 

For Alltech ® Prevail Carbohydrate ES columns, see page 47. 

5 

10 15 Min. 

Column: Alltech ® Carbohydrate Cation, 10µm, 6.5 x 300mm 

(Part No. 70057) 

Mobile Phase: Water 



Detector: Alltech ELSD 

Carbohydrate Cation All-Guard Cartridges 


Carbohydrate Cation Guard Cartridges 4.6 x 7.5mm 3 96109 


80101 



Mixed-Mode All-Guard Cartridges* 

Packing, 5µm i.d. x Length Qty. Part No. 

C8/Anion 4.6 x 7.5mm 3 96147 

C8/Cation 4.6 x 7.5mm 3 71379 

C18/Cation 4.6 x 7.5mm 3 71380 


ea 80101 



related product 

Need a column heater? 

See pages 16 and 17. 


Doing carbohydrate analysis? 

Alltech ® 3300 ELSD is more 

sensitive than RI. See pages 5–10. 

6219 

6947B 

68 



Vydac ® Venture ® Immunoaffinity Columns 

Repetitive-Use, Silica-Based Immunoaffinity Columns 

Venture ® immunoaffinity HPLC columns provide an easy and quick way to purify and concentrate analytes from complex food, 

environmental, and biological matrices for fast, accurate measurement. 

6908 

Vydac ® Venture ® immunoaffinity columns use ICEtech (Inert Coating Enhancement Technology, U.S. Patent No. 6,802,966) 

silica processing to eliminate non-specific binding on the silica surface. This enables Venture ® columns to be the first affinity 

matrix to take advantage of silica’s rigid porous structure, making direct coupling of the columns with other modes 

of chromatography, such as reversed-phase HPLC feasible. 

Benefits 

Rapid Analysis 

Excellent Accuracy and 

Precision 

Highly Selective 

Features 

• No laborious sample preparation 

procedures 

• Fast binding kinetics 

• Wide-pore silica support allows online 

coupling with HPLC for real time 

direct analysis 

• Quantitative recovery of analyte from 

IAC column 

• High sensitivity due to high volume 

injection 

• Precision comparable with 

conventional HPLC methods 

• Specially treated wide-pore silica 

eliminates non-specific interactions 

• Secondary separation reduces errors 

due to cross reactivity 

• Highly selective antibodies toward 

target analyte 

Long Column Lifetime 

• Analyze over 200 samples on a single 

column without column deterioration 

due to the high ligand stability of the 

Venture ® IAC columns 

Analysis of Difficult Matrices • Venture ® IAC columns isolate, 

concentrate and purify targeted 

sample components from dilute and 

complex matrices in one step 

Significant Cost Reductions • Costs reduced by as much as 90% 

Automated Analysis 

• Long column lifetime decreases 

material costs to less than $5.00 

per analysis 

• Rapid analysis and automation 

reduces technician time 

• Significant reduction in solvent use 

• Less solvent waste disposal costs 

• HPLC format allows automation for 

high sample throughput 

Venture ® Immunoaffinity Columns 

Product Name Target Analyte i.d. and length Part No. 

Mycotoxins Analysis 

Venture ® AF Aflatoxin 2.1 x 50mm 5120313 

Venture ® OT Ochratoxin A 2.1 x 50mm 5135954 

Food Nutrient Analysis 

Venture ® FA Folic Acid 2.1 x 50mm 5135952 

Venture ® B12 Vitamin B12 2.1 x 50mm 5120201 

Venture ® BF Folic Acid and Vitamin B12 2.1 x 50mm 5135951 

Acrylamide Analysis 

Venture ® AC Acrylamide 2.1 x 50mm 5135953 

Lactoferrin Analysis 

Venture ® LTF Lactoferrin 2.1 x 50mm 5120400 

Endocrine Disruptors Analysis 

Venture ® EE2 17α-Ethynyl Estradiol 2.1 x 50mm 5120404 

Venture ® E2 17β-Estradiol 2.1 x 50mm 5120403 

Venture ® E1 Estrone 2.1 x 50mm 5120405 

Venture ® BPA Bisphenol A 2.1 x 50mm 5120406 

Pollutants Analysis 

Venture ® CPA Chlorophenoxy Acetic Acid 2.1 x 50mm 5120407 

Herbicides 

Venture ® PVH Phenylurea Herbicides 2.1 x 50mm 5120408 

Venture ® OPP Organophosphorous 2.1 x 50mm 5120410 

Pesticides 

Venture ® VCZ Vinclozolin Fungicide 2.1 x 50mm 5120411 

Steroid Hormones Analysis 

Venture ® TT Testosterone 2.1 x 50mm 5120401 

Venture ® NT Nortestosterone 2.1 x 50mm 5120402 


Vydac ® Denali ® C18 Reversed Phase 

Denali ® columns contain a new-generation silica-based 120Å monomeric C18 adsorbent 

based on the same bonding technology employed in Vydac ® Everest ® 300Å columns. This new 

technology improves C18 surface coverage and deactivates residual silanols. Previously, the 

best monomeric C18 chemistries had carbon coverage in the 2.8 to 3.6µmoL/m 2 range. Denali ® 

C18 has coverage in excess of 4µmoL/m 2 and approximates the theoretical limit based on 

surface area. 

7110 

Vydac ® Denali ® C18 Reversed-Phase 238DE C18 

Columns 

Recommended Guards 

Particle Size i.d. 50mm 100mm 150mm 250mm Guard Kit 1 Guard Cartridge 2 

3µm 2.1mm 3 238DE3205 238DE3210 — — — — 

4.6mm 3 238DE3405 238DE3410 — — 238GK34DE 238GD34DE 

5µm 1.0mm 3 238DE5105 238DE5110 238DE5115 238DE51 238GK51DE 238GD51DE 

2.1mm 3 238DE5205 238DE5210 238DE5215 238DE52 238GK52DE 238GD52DE 

4.6mm 3 238DE5405 238DE5410 238DE5415 238DE54 238GK54DE 238GD54DE 

NOTE: Additional column diameters and lengths are available on request. Please contact Grace Davison Discovery Sciences to discuss your requirements. 

1 

A guard kit includes a holder and one guard cartridge. 2 Guard cartirdge units include two guard cartridges. 3 Titanium frits are standard in column diameters 4.6mm and smaller. 




Vydac ® 201TP and 202TP C18 

Specialty Reversed Phases for PAH and Vitamins 

• 201TP: The standard for PAH analysis in all types of environmental samples. Vydac ® 201TP 

columns separate the EPA 16 priority pollutants in less than 20 minutes. 

• 202TP3405: Rapid analysis to separate the 16 priority pollutant PAHs in under 10 minutes 

• 202TP54 and 202TP5415: For the analysis of derivatized PAHs 

Vydac ® 201TP and 202TP columns were developed specifically for the separation and quantification of PAHs required by 

environmental regulations, current and future. Beyond the 16 EPA priority pollutant PAHs, Vydac ® PAH columns are used to 

separate many other PAHs, such as methylated naphthalenes. 

Vydac ® 201TP and 202TP C18 Specifications 


201TP C18 Silica Spheroidal 5, 7, 10, 10–15, 15–20µm 300Å 70–90m 2 /g 8% Polymeric No L1 


Vydac ® 201TP 

Vydac ® 201TP HPLC columns have long been the standard for the analysis of PAHs in water, air, soil, automotive exhaust, 

and food. They were used to establish standard reference materials, measure air quality, measure PAHs in sediments, 

quantify PAHs in food, and study high molecular weight PAHs. They have also been used in the study of shape selectivity 

of reversed-phase materials. 

Priority Pollutants PAHs 

In Accordance with 

EPA Methods 505, 550.1, 610, and 8310 

1. Naphthalene 

2. Acenaphthylene 

3. Acenaphthene 

4. Fluorene 

5. Phenanthrene 

6. Anthracene 

7. Fluoranthene 

8. Pyrene 

9. Benz[a]anthracene 

10. Chrysene 

11. Benzo[b]fluoranthene 

12. Benzo[k]fluoranthene 

13. Benzo[a]pyrene 

14. Dibenz[ah]anthracene 

15. Benzo[ghi]perylene 

16. Indeno[1,2,3-cd]pyrene 

11 

PAHs Beyond the EPA Priority Pollutants 



3. 1-Methylnaphthalene 



6. Fluorene 


8. Anthracene 


10. Pyrene 

11. Benzo[c]phenanthrene 

12. Cyclopenta[cd]pyrene 

2 

8 


14. Chrysene 

15. Benzo[b]naptho[2,1-d]thiopen 

16. 7,12-Dimethylbenz[a]anthracene 

17. Benzo[e]pyrene 







7110 

10 

1 

7 

18 

1 

2 

3 

4 

5 6 7 

8 

9 

12 

13 16 

15 

14 

3 45 6 

9 

10 

1112 13 14 

15 16 17 

19 

20 

22 

21 

23 

0 1 3 5 7 9 11 13 15 Min. 

0 4 8 12 16 20 24 28 32 Min. 

Column: 

Flow Rate: 

Mobile Phase: 

Gradient: 

Detector: 

Vydac ® C18, 5µm, 4.6 x 150mm 

(Part No. 201TP5415) 

1.5mL/min 

A: Water B: ACN 

Hold 50% B for 3min, then 50 to 100% B in 7min 

UV at 254nm 

Column: Vydac ® C18, 5µm, 4.6 x 150mm 

(Part No. 201TP5415) 


Mobile Phase: A: Water B: ACN 

Gradient: 50 to 100% B over 30min 







70 



Vydac ® 201TP (continued) 

Vitamins 

Vydac ® 201TP columns also have application in the analysis 

of carotenoids, retinoids, and vitamins. 

Vydac ® 202TP 

Built on top of the success of 201TP, 202TP columns are 

ideal for derivatized PAH samples or high throughput PAH 

analyses. 

Retinol, a-Tocopherol, and b-Carotene in Serum 

1. All-trans-retinol (vitamin A) 

2. Tocol 

3. g-Tocopherol 

4. a-Tocopherol (vitamin E) 

5. Lutein 

6. Zeaxanthin 

7. Cryptoxanthin 

8. a-Carotene 

9. All-trans-b-carotene 

10. Cis-b-carotene 

325 nm 295 nm 450 nm 

7 

1 

2 

3 

4 

5 

6 

0 4 8 12 16 20 24 28 32 Min. 

8 

9 

10 




4. Fluorene 


6. Anthracene 


8. Pyrene 

1 

2 

Rapid Analysis of Polyaromatic Hydrocarbons 

3 

4 

5 

6 

7 

8 


10. Chrysene 







9 10 11 

12 

13 14 

15 

16 


Column: 

Flow Rate: 

Mobile Phase: 

Gradient: 

Detector: 

Vydac ® 201TP C18, 5µm, 4.6 x 250mm 

(Part No. 201TP54) 

1.5mL/min 

A: 15:75:10 Buffer/Methanol/n-Butanol 

B: 2:88:10 Buffer/Methanol/n-Butanol 

Buffer = Aqueous 0.02 M NH4OAc, pH 3.5 

100% A for 3min, then 0 to 100% B over 15min, 

then hold 100% B 

UV, programmed wavelengths 

0 1 2 3 4 5 6 7 8 Min. 

Column: Vydac ® 202TP C18, 5µm, 4.6 x 50mm 

(Part No. 202TP3405) 


Mobile Phase: A: Water B: ACN 

Gradient: 40 to 95% B in 8min 



Chromatogram reproduced with author’s permission. From W.A. MacCrehan and 

E. Schonberger, Clin. Chem., 33(9), 1585-1592 (1987). 

201TP C18 Analytical Columns 

Columns 



5µm 1.0mm 201TP5105 — 201TP5115 201TP51 201GK51T 201GD51T 

2.1mm 201TP5205 201TP5210 201TP5215 201TP52 201GK52T 201GD52T 

3.2mm — — 201TP5315 201TP53 201GK54T 201GD54T 

4.6mm 201TP5405 201TP5410 201TP5415 201TP54 201GK54T 201GD54T 

10µm 4.6mm — — 201TP10415 201TP104 201GK104T 201GD104T 

1 

A guard kit includes a holder and one guard cartridge. 2 Guard cartridge units include two guard cartridges. 

202TP High-Carbon-Load C18 Analytical Columns 

Columns 



3µm 3.2mm — — 202TP3315 — — — 

4.6mm 202TP3405 202TP3410 — — — — 

5µm 4.6mm — — 202TP5415 202TP54 202GK54T 202GD54T 

10µm 4.6mm 202TP10405 — — — 202GK104T 202GD104T 

1 

A guard kit includes a holder and one guard cartridge. 2 Guard cartridge units include two guard cartridges. 




Astec Amino and 

Reversed-Phase Columns 

• Durable vinyl alcohol copolymer 

• Stable from pH 2–13 

• Stable and reproducible performance 

Choose Astec amino columns for separating mono- and 

oligosaccharides. Astec’s polymer base makes these columns 

much more stable than silica-based amino columns. 

Choose Astec reversed-phase columns for amines and high 

recoveries of proteins and peptides. Because there are no 

silanols, elution order is always based on hydrophobicity 

of the analytes rather than polar interactions with the base 

material. High recovery of proteins and peptides is typical 

even at low sample loads. 

1 

2 

3 4 

Mono-, Di-, and Trisaccharides 

5 6 

9103 

1. Rhamnose 

2. Fructose 

3. Glucose 

4. Sucrose 

5. Maltose 

6. Raffinose 

Astec Cyclobond Columns 

For Chiral Separations 

• Versatile chiral selectors 

• High-purity 5µm spherical silica 

Cyclodextrin Phases 

Designation 

Substituent 

I 2000 

β-Cyclodextrin 

I 2000 Ac 

β-Cyclodextrin Acetylate 

I 2000 RSP β-Cyclodextrin R,S-Hydroxypropyl Ether 

I 2000 DMP β-Cyclodextrin 3,5-Dimethylphenyl Carbamate 

II 

γ-Cyclodextrin 

Cyclobond I 2000 has the broadest applicability, and 

is ideal for small analytes in pharmaceutical, chemical, 

and environmental applications. 

Cyclobond 1 2000 Ac is ideal for aromatic alcohols 

or amines that are chiral on the alpha or beta carbon. 

Cyclobond I 2000 RSP is a general-purpose chiral 

stationary phase. It can separate non-aromatic structures 

such as t-boc amino acids. 

Cyclobond DMP shows good selectivity when the 

chiral centers are part of a ring structure or on the alpha 

carbon. This phase is ideal for derivatized amines such 

as amphetamine ACQ. 

Cyclobond II is useful for isomeric compounds based 

on anthracene, chrysene, and pyrene ring structures. 

Some applications include steroids, porphyrins, and FMOC 

amino acids. 

0 

10 20 30 Min. 

Column: Astec Amino, 5µm, 4.6 x 250mm (Part No. 16012) 



Detector: RI 

Astec Amino and Reversed-Phase 300Å Columns 









Astec Amino and Reversed-Phase Guard Columns 


C18, 5µm 4.6 x 10mm ea 28180 

C8, 5µm 4.6 x 10mm ea 28181 

C4, 5µm 4.6 x 10mm ea 28182 

Amino, 5µm 4.6 x 10mm ea 28187 

Astec Cyclobond Columns 


I 2000, 5µm Analytical 4.6 x 100mm 400101 


I 2000 Ac, 5µm Analytical 4.6 x 250mm 410121 

I 2000 RSP, 5µm Analytical 4.6 x 250mm 411121 

I 2000 DMP, 5µm Analytical 4.6 x 250mm 412111 

II Analytical 4.6 x 100mm 400201 


Astec Cyclobond Guard Cartridges* 

Packing, i.d. x Length Qty. Part No. 

I 2000, 5µm 4.0 x 20mm ea 430102 

I 2000 Ac, 5µm 4.0 x 20mm ea 430103 

I 2000 RSP, 5µm 4.0 x 20mm ea 430105 

I 2000 DMP, 5µm 4.0 x 20mm ea 430108 

II 4.0 x 20mm ea 430109 

Astec Guard Cartridge Holder ea 11014 


72 



Astec Chirobiotic 

HPLC Columns 

For Multi-Mode Chiral Separations 

• Bonded macrocyclic glycopeptide phases 

• Three complimentary selectivities 

• High-purity 5µm spherical silica 

Chirobiotic columns demonstrate a broad selectivity in 

reversed phase, normal phase, and polar organic modes. 

This gives Chirobiotic columns the ability to separate a 

greater variety of chiral analytes than columns that can only 

operate in one mode. 

Chirobiotic V 

Chirobiotic V Specifications 

Ligand: Vancomycin 

Ideal For: Neutral molecules, amides, acids, esters, cyclic amines 

Chirobiotic V has a selectivity similar to glycoprotein phases 

while also being stable from 0–100% organic modifiers. New 

Chirobiotic V2 has enhanced selectivity and capacity in the 

polar organic mode, and increased capacity. 

Naproxen 

8769 

CH 3 

CHCOH 

O 

H 3 CO 

6 8 10 12 Min. 

Column: Chirobiotic V, 4.6 x 250mm (Part No. 11024) 

Mobile Phase: THF:0.1% Triethylammonium Acetate, pH 7 (10:90) 



Phenylalanine 

8770 

O 


Chirobiotic T 

Chirobiotic T Specifications 

Ligand: Teicoplanin 

Ideal For: Underivatized amino acids, n-derivatized amino acids, 

carboxylic acids, phenols, neutral aromatics, cyclic aromatics 

with aliphatic amines 

Chirobiotic T is an excellent alternative to crown ether and 

ligand exchange for amino acids and hydroxy acids. New 

Chirobiotic T2 has enhanced selectivity and capacity in the 

polar organic mode. 

Chirobiotic R 

Chirobiotic R Specifications 

Ligand: Ristocetin A 

Ideal For: Anionic molecules, di- and tri-peptides, α-hydroxy acids, 

substituted aliphatic acids, aromatic esters, chiral alcohols, 

secondary and tertiary amines 

Chirobiotic R is the largest and most complex of the 

Chirobiotic ligands. Sugar moieties, a peptide chain, and 

additional ionizable groups give this structure the complexity 

and diversity to separate a wide variety of analytes. 

4 6 8 10 12 Min. 

NH 2 

Column: Chirobiotic T, 4.6 x 250mm (Part No. 12053) 

Mobile Phase: Ethanol:Water (80:20) 



Astec Chirobiotic Columns 


Chirobiotic V Analytical 4.6 x 50mm 11346 



Chirobiotic V2 Analytical 4.6 x 100mm A15022 

Analytical 4.6 x 150mm A15023 


Prep 10 x 250mm A15034 

Prep 21.2 x 250mm A15044 

Chirobiotic T Analytical 4.6 x 50mm 11349 



Chirobiotic T2 Analytical 4.6 x 100mm A16022 



Prep 10 x 250mm A16034 

Prep 21.2 x 250mm A16044 

Chirobiotic R Analytical 4.6 x 50mm 12514 



Astec Chirobiotic Guard Cartridges* 


Chirobiotic V, 5µm 4.0 x 20mm ea 11019 

Chirobiotic T, 5µm 4.0 x 20mm ea 12445 

Chirobiotic R, 5µm 4.0 x 20mm ea 12522 

Astec Guard Cartridge Holder ea 11014 


OH 



hplc columns | small and large molecule 

74 

Brownlee Columns 

Economical MPLC Cartridge System with 

Reusable Hardware 

• Finger-tight modular design 

• Reusable end assemblies with interchangeable holders 

• Guards fit conventional columns or MPLC hardware 

The Brownlee MPLC Cartridge system is convenient 

and easy to use. Decide which column length you prefer, 

and purchase the hardware and the disposable packed 

cartridges separately. 

Cartridge Hardware 

Brownlee MPLC Cartridge Hardware 

Photo Description Part No. 

MPLC Cartridge Holders 

30mm 140200 

5424 

MPLC Cartridge Accessories 

0647B 

Brownlee MPLC NewGuard Hardware 

Photo Description Part No. 

NewGuard Cartridge 140601 

Holders NewGuard Holder 

0647E for use with Conventional 

Columns 

220mm NewGuard 140410 

System Holder for Direct 

Coupling of 220mm 

0647F 

Analytical Cartridge with a 

NewGuard Cartridge 

NewGuard End Assembly 140600 


100mm 140230 

220mm 140400 

MPLC Cartridge Union 140300 

MPLC Cartridge End 1402501 

0647C Assembly 

MPLC Cartridge Holder Bodies 

30mm 140203 

0647D 

100mm 140003 

220mm 140403 

Universal MPLC Cartridge Holder Kit 

Includes: 30-, 100-, and 220mm MPLC Cartridge Holder 

Bodies (E), 1 MPLC Cartridge Union (C), 2 End Assemblies 

(D), and 1 NewGuard End Assembly (H) 

Brownlee MPLC Cartridge Hardware Seals 

Description 

Analytical Holder Seal Replacement Kit 

Includes Tool and 2 Seals 

Analytical Seals, 2/pk 

Seal Replacement Tool Required 

NewGuard System 

0647G 

0647A 

140530 

Part No. 

140260 

140216 

Brownlee NewGuard Cartridges* 


RP-18 3 141004 

RP-8 3 141003 

Cyano 3 141008 

Amino 3 141007 

Silica 3 141012 

Anion (AX-300) 3 141009 

*NewGuard cartridge holder required (Part No. 140601). 

Spheri-5 and Spheri-10 Cartridges 

• Rugged and reproducible 

• Use with MPLC cartridge hardware 

• 80Å, spherical silica 

RP-18 and RP-8 phases are monomeric for optimum mass 

transfer kinetics and high efficiency. The ODS phase is 

polymeric and more suitable for acidic mobile phases. 

Brownlee Spheri-5 Cartridges* 


ODS (C18), 5µm 2.1 x 30mm 2 141061 

2.1 x 100mm ea 141063 

2.1 x 220mm ea 141065 

4.6 x 30mm 2 141060 

4.6 x 100mm ea 141062 

4.6 x 220mm ea 141064 

RP-18, 5µm 2.1 x 30mm 2 141052 

2.1 x 100mm ea 141054 

2.1 x 220mm ea 141056 

4.6 x 100mm ea 141053 

4.6 x 220mm ea 1410551 

RP-8, 5µm 2.1 x 30mm 2 141028 

2.1 x 100mm ea 141030 

2.1 x 220mm ea 141032 

4.6 x 30mm 2 141027 

4.6 x 100mm ea 141029 

4.6 x 220mm ea 141031 

Cyano, 5µm 4.6 x 30mm 2 141083 

4.6 x 100mm ea 141085 

4.6 x 220mm ea 141087 

Amino, 5µm 4.6 x 30mm 2 141177 

4.6 x 100mm ea 141179 

4.6 x 220mm ea 141181 

Silica, 5µm 2.1 x 30mm 2 141194 

4.6 x 30mm 2 141193 

4.6 x 100mm ea 141195 

4.6 x 220mm ea 141197 

*Brownlee MPLC cartridge holder required. 

Brownlee Spheri-10 Cartridges* 


RP-18, 10µm 4.6 x 30mm 2 141043 

4.6 x 100mm ea 1410451 

RP-8, 10µm 4.6 x 30mm 2 141020 

4.6 x 100mm ea 141022 

Silica, 10µm 4.6 x 30mm 2 141186 

*Brownlee MPLC cartridge holder required. 

Aquapore DEAE WAX Cartridges 

• Large pore for macromolecules 

Choose AX-300 with its DEAE functional group for weak anion 

exchange applications with proteins and peptides. 

Brownlee Aquapore Cartridges* 


AX-300, 7µm 4.6 x 30mm 2 141212 

4.6 x 100mm ea 141214 

*Brownlee MPLC cartridge holder required.


Jordi Columns 

Normal- and Reversed-Phase 

• Rugged—use at high temperatures, from pH 0–14, 

with any solvent 

• Silanol Free—analyze polar compounds without 

silanol effects 

Jordi normal-phase and reversed-phase columns are 

made from highly pure 5µm DVB, for extremely durable 

and efficient phases. 

Jordi Specifications 

Phase Particle Size Pore Size Description 

RP-DVB 5µm 500Å, 1000Å Reversed-phase, 

not bonded 

C18-DVB 5µm 500Å Reversed-phase, 

C18 bonded 

Polyamine-DVB 5µm 500Å Normal-phase 

RP-DVB 

RP-DVB is a reversed-phase non-polar packing with a high 

degree of aromatic character. SM-500, with a 500Å pore, is 

recommended for most applications. LM-1000, with a 1000Å 

pore, is ideal for molecules 100,000 Daltons or larger. 

C18-DVB 

C18-DVB has C18 chains bonded to the DVB base. These 

columns are more hydrophobic than traditional C18 columns 

and have different selectivities due to the absence of silanols. 

Polyamine-DVB 

Polyamine-DVB is a normal-phase column for simple sugar 

and polysaccharide applications. The DVB-based packing 

provides a non-reactive amine surface that lasts longer and 

equilibrates faster than traditional silica-based amine columns. 

Polyamine-DVB columns can be used with aqueous mobile 

phases, even 1M NaOH. Ideal for carbohydrate analyses 

requiring sharp peaks and short run times. 

Jordi HPLC Columns 


RP-DVB (SM-500Å), 5µm Analytical 4.6 x 150mm 1005500 


RP-DVB (LM-1000Å), 5µm Analytical 4.6 x 150mm 1005580 


C18-DVB (500Å), 5µm Analytical 4.6 x 150mm 18500 


Polyamine-DVB (500Å), 5µm Analytical 4.6 x 250mm 1005900 

Jordi All-Guard Cartridges* 


RP-DVB (SM-500Å), 5µm 4.6 x 7.5mm 3 1005595 

RP-DVB (LM-1000Å), 5µm 4.6 x 7.5mm 3 1005596 

C18-DVB (500Å), 5µm 4.6 x 7.5mm 3 1005601 

Polyamine-DVB (500Å), 5µm 4.6 x 7.5mm 3 1005615 


ea 80101 




Looking for column heaters? 


GPC Columns 

Organic GPC 

• Rugged—highly crosslinked DVB for broad temperature 

stability and solvent compatibility 

• Powerful—high pore volume for greater resolution from 

a single column 

Jordi Organic and Aqueous GPC Specifications 

Pore Size Pressure Limit MW Range (Daltons) 

100Å 8000psig 100–5000 

500Å 8000psig 100–10,000 

103Å 8000psig 100–25,000 

104Å 2000psig 100–2,000,000 

105Å 2000psig 10,000–10,000,000 

Mixed Bed 2000psig 100–>10,000,000 

Jordi HPLC columns, offer unparalleled resistance to 

shrinking and swelling, as well as better temperature and 

solvent compatibility than traditional ps-DVB phases. 

The high pore volume speeds up your analyses. A single 

10 x 500mm Jordi column replaces three 7.8 x 300mm ps-DVB 

columns and separates the same sample in 40% less time. 

Aqueous GPC 

Glucose-DVB packings are rugged, hydrophilic packings for 

separating polar compounds. Glucose units are bonded to 

the DVB base to yield a hydrophilic surface. Glucose-DVB 

columns equilibrate faster than silica-based columns. 

Jordi Organic GPC Columns* 

Pore Size Format i.d. x Length Part. No. 

100Å, 5µm Semi Prep 7.8 x 300mm 10510 

Prep 10 x 250mm 100561 


Prep 10 x 250mm 100565 

Prep 10 x 500mm 100567 


Prep 10 x 250mm 100569 


Prep 10 x 250mm 100573 


Prep 10 x 250mm 100577 

Mixed-Bed Linear, 5µm Semi Prep 7.8 x 300mm 10515 

Prep 10 x 250mm 100581 

Prep 10 x 500mm 100583 

*Jordi GPC columns are constructed from Type 316 stainless steel. All tubing 

connections are 1/16" female. Columns are shipped in Chloroform unless otherwise 

requested when placing order. 

Jordi Aqueous GPC Columns 

Pore Size Format i.d. x Length Part No. 


Prep 10 x 250mm 100761 


Prep 10 x 250mm 100765 


Prep 10 x 250mm 100769 


Prep 10 x 250mm 100773 


Prep 10 x 250mm 100777 

Mixed-Bed Linear, 5µm Semi Prep 7.8 x 300mm 10522 

Prep 10 x 250mm 100781 

Prep 10 x 500mm 100783 


6219 




Merck LiChrosorb ® and LiChrospher ® Columns 

• LiChrosorb ® —irregular silica for larger surface areas 

• LiChrospher ® —spherical silica for higher stability 

and reproducibility 

All Merck columns are packed by Grace to our highest 

QC standards. Custom columns are available. 

LiChrosorb ® HPLC Columns 


RP-18, 5µm Solvent Reducer 3.0 x 150mm 38513 

Solvent Reducer 3.0 x 250mm 38514 


RP Select B, 5µm Analytical 4.6 x 150mm 228700 


RP-8, 5µm Solvent Reducer 3.0 x 150mm 38515 




Si-60, 10µm Analytical 4.6 x 250mm 8369 


Melatonin 

0 2 4 6 8 Min. 

1 

Column: LiChrospher ® RP-18, 5µm, 4.6 x 150mm 

(Part No. 228695) 

Mobile Phase: 25mM KH2PO4, pH2.75:MeOH (60:40) 



9055 

1. Melatonin 

Alltech ® All-Guard 

System—change cartridges 

in seconds without tools. 

LiChrospher ® HPLC Columns 


RP-18, 3µm Analytical 4.6 x 150mm 228688 



RP-18 EC, 3µm Analytical 4.6 x 150mm 228686 

RP-18 EC, 5µm Analytical 4.6 x 150mm 228694 




RP-8 EC , 5µm Analytical 4.6 x 150mm 228697 


RP-Select B, 5µm Analytical 4.6 x 150mm 228611 




Diol-100, 5µm Analytical 4.6 x 150mm 228691 




1656 

LiChrosorb ® All-Guard Cartridges* 


RP-18, 5µm 3.0 x 7.5mm 3 96374 

4.6 x 7.5mm 3 96171 

RP-Select B, 5µm 4.6 x 7.5mm 3 96181 

RP-8, 5µm 3.0 x 7.5mm 3 96373 

4.6 x 7.5mm 3 96173 

Amino, 5µm 4.6 x 7.5mm 3 96175 

Silica, 5µm 4.6 x 7.5mm 3 96201 


ea 80101 



LiChrospher ® All-Guard Cartridges* 


RP-18, 5µm 4.6 x 7.5mm 3 96185 

RP-18 Endcapped, 5µm 4.6 x 7.5mm 3 96183 

RP-8, 5µm 4.6 x 7.5mm 3 96189 

RP-8 Endcapped, 5µm 4.6 x 7.5mm 3 96187 

RP Select B, 5µm 4.6 x 7.5mm 3 96425 

Amino, 5µm 4.6 x 7.5mm 3 96191 

Diol, 5µm 4.6 x 7.5mm 3 96195 

Silica, 5µm 4.6 x 7.5mm 3 96201 


ea 80101 



76 



Macherey-Nagel Nucleosil ® Columns 

• 100Å, high-purity silica 

• Narrow particle size distribution 

• A choice of phases for any application 

All Nucleosil ® columns are packed by Grace to our highest 

QC standards. 

C18 AB is polymerically bonded specifically for reversedphase 

chromatography of acidic and basic compounds. 

It has a 25% carbon load for strong retention. 

1 

0 1 

2 

3 

4 

2 

SRM 869 

3 

5 6 7 8 9 Min. 

Column: Nucleosil ® C18 AB, 5µm, 4.6 x 150mm 

(Part No. 11652) 

Mobile Phase: (85:15) Acetonitrile:Water 




4 

9320 

1. Acetone 

2. Phenanthroanthrene 

3. 1,2:3,4:5,6:7, 

8-Tetrabenzonaphthalene 


Nucleosil ® HPLC Columns 


Waters ® 

Fittings 

Part No. 






C18 AB, 5µm Analytical 4.6 x 150mm 11652 — 
















SA, 5µm Analytical 4.6 x 150mm 228498 228504 


SA, 10µm Analytical 4.6 x 250mm 228496 228502 

SB, 5µm Analytical 4.6 x 150mm 228501 228507 


SB, 10µm Analytical 4.6 x 250mm 228499 228505 


Opium Alkaloids 

5243 

Alltech ® All-Guard 

System—change cartridges 

in seconds without tools. 

1 

2 

3 

4 

0 4 8 12 16 Min. 

5 

6 

Column: Nucleosil ® CN, 10µm, 4.6 x 250mm 

(Part No. 89281) 

Mobile Phase: 80%15mM Ammonium Acetate pH 5.8 

10% Acetonitrile 10% Dioxane 




1. Morphine 

2. Codeine 

3. Cryptopine 

4. Thebaine 

5. Narcotine 

6. Papaverine 

Nucleosil ® All-Guard Cartridges* 


C18, 5µm 4.6 x 7.5mm 3 96210 

C8, 5µm 4.6 x 7.5mm 3 96211 

Phenyl, 5µm 4.6 x 7.5mm 3 96214 

Cyano, 5µm 4.6 x 7.5mm 3 96213 

Amino, 5µm 4.6 x 7.5mm 3 96212 

Silica, 5µm 4.6 x 7.5mm 3 96215 

SA, 5µm 4.6 x 7.5mm 3 89340 

SB, 5µm 4.6 x 7.5mm 3 89345 



ea 80101 

1656 


more info 

For more HPLC Applications, see pages 395–453. 








Whatman Partisil Columns 

All Whatman Partisil columns are packed by Grace to our 

highest QC standards. Partisil ® columns use 85Å, irregular 

silica with a surface area of 350m 2 /g. 

Partisil PAC is a polar amino cyano phase with secondary 

amino groups for thermal and chemical stability. It separates 

primarily by reversed-phase or weak anion exchange. 

Partisil HPLC Columns 


Waters ® 

Fittings 

Part No. 

ODS, 10µm Analytical 4.6 x 250mm 8246 8247 

ODS-2, 5µm Analytical 4.6 x 250mm 12571 12572 






PAC, 5µm Analytical 4.6 x 250mm 8676 8678 

PAC, 10µm Analytical 4.6 x 250mm 8261 8262 






Partisil All-Guard Cartridges* 


ODS, 5µm 4.6 x 7.5mm 3 96252 

ODS-2, 5µm 4.6 x 7.5mm 3 96253 

ODS-3, 5µm 4.6 x 7.5mm 3 96254 

C8, 5µm 4.6 x 7.5mm 3 96255 

PAC, 5µm 4.6 x 7.5mm 3 96256 

Silica, 5µm 4.6 x 7.5mm 3 96257 

SAX, 5µm 4.6 x 7.5mm 3 96259 

SCX, 5µm 4.6 x 7.5mm 3 96258 


ea 80101 



Whatman Partisphere ® 

WVS Columns 

Whatman’s Partisphere ® WVS columns have built-in void 

compensators that help you extend the column lifetime. 

Use the piston-like plungers at each end of the column to 

compress the bed and seal any voids that develop over time. 

Choose your column from the table below, and purchase 

reusable WVS endfittings separately. 

Partisphere ® WVS HPLC Cartridges* 




PAC, 5µm Analytical 4.6 x 125mm 46210508 


SAX, 5µm Analytical 4.6 x 125mm 46210505 

SCX, 5µm Analytical 4.6 x 125mm 46210507 

WVS Reusable Endfittings, 2/pk 46310001 

*Reusable endfittings required. 

Partisphere ® WVS Guard Cartridges* 


C18/C8, 5µm 5 x 17mm 5 46410002 

PAC, 5µm 5 x 17mm 5 46410008 

Silica, 5µm 5 x 17mm 5 46410001 

SAX, 5µm 5 x 17mm 5 46410005 

SCX, 5µm 5 x 17mm 5 46410007 

Guard Cartridge Holder 19 x 40mm ea 46311004 


more info 

For HPLC Applications, see pages 395–453. 


Need HPLC tubing? 







Looking for high-pressure fittings? 


5535 

6673 

78 



Polymer Labs Columns 

Outstanding Durability and Stability 

• Ideal for elevated temperature and pressure applications 


PLRP-S is a polystyrene divinylbenzene copolymer designed 

specifically for reversed-phase HPLC. PLRP-S columns are 

extremely stable, performing over a wide pH range and at 

elevated temperatures and pressures. 

PLRP-S Choice of Pore Sizes: 

• 100Å for small molecules, peptides, and nucleotides 

• 300Å for polypeptides and globular proteins 

• 1000Å for fibrous proteins 

• 4000Å for the analysis of very large biomolecules or 

high-speed/high-resolution separations 

PLRP-S HPLC Specifications 

Base Material 

Polystyrene Divinylbenzene 

Organic Modifier 0 to 100% 

Particle Size 3, 5, 8, 10µm 

Pore Size 

100, 300, 1000, 4000Å 

pH Range 1–13 

Max. Temp. 80ºC 

Max. Pressure 

3000psig 

Max. Flow Rate 

4mL/min 

1 

2 

4 

3 

0 2 4 6 8 10 12 Min. 

Column: 

Mobile Phase: 

Gradient: 

Flow Rate: 

Detector: 

Enkephalins 

5 

PLRP-S 100Å, 5µm, 4.6 x 250mm 

(Part No. 1512-5500) 

A: 0.1% TFA, 1% IPA in Water 

B: 0.1% TFA, 1% IPA in ACN 

Linear 15 – 85% B in 15min 

1.0mL/min 

UV at 220nm 

7253 

1. Methionine Enkephalin Sulphoxide 

2. Methionine Enkephalin Lysine 

3. Methionine Enkephalin 

4. D-ALA2 Methionine Enkephalin 

5. D-TRP Methionine Enkephalin 

PLRP-S HPLC Columns 


100Å, 3µm Microbore 2.1 x 50mm 1912-1300 

Microbore 2.1 x 150mm 1912-3300 

Analytical 4.6 x 50mm 1512-1300 



Microbore 2.1 x 150mm 1912-3500 

Microbore 2.1 x 250mm 1912-5500 




100Å, 8µm Analytical 4.6 x 150mm 1512-3800 



Microbore 2.1 x 150mm 1912-3301 




Microbore 2.1 x 150mm 1912-3501 

Microbore 2.1 x 250mm 1912-5501 




300Å, 8µm Analytical 4.6 x 150mm 1512-3801 





Microbore 2.1 x 150mm 1912-3802 







Microbore 2.1 x 150mm 1912-3803 



PLRP-S Guard Columns 


PLRP-S, 5µm 3 x 5mm 2 1512-1503 

Guard Column Holder ea 1310-0016 

more info 

For more large molecule columns, see pages 84–97. 



See page 16–17. 


6219 




Shodex ® Columns 

Aqueous Size Exclusion 

• High efficiency and high mass recovery 

• Separate biopolymers by their effective size in solution 

• Stable sugar GFC columns 

Shodex ® Ohpak and Sugar columns are designed for highresolution 

separations of water-soluble compounds such as 

organics, inorganics, oligomers, and polymers. Three different 

gel filtration size exclusion materials are available. 

Shodex ® Sugar columns use a sulfonated gel with a 

sodium counter ion for separation of mono, di, oligo, and 

polysaccharides, starches, and celluloses. 

Proteins by GFC 

1 

2 3 4 5 

9162 

1. Glutamate Dehydrogenase (290k) 

2. Lactate Dehydrogenase (142k) 

3. Enolase (67k) 

4. Adenylate Kinase (32k) 

5. Cytochrome (12.4k) 

Aqueous Size Exclusion Columns 

Packing Series Description Applications 

OHpak SB-800 Polyhydroxymethacrylate General purpose GFC of 

water-soluble polymers, 

proteins, and enzymes 

Protein KW-800 Porous Silica Gel GFC of proteins, 

glycoproteins, and peptides 

Sugar KS-800 

1 

Sulfonated PS Gel 

(Na+ counter ion) 

2 

3 4 5 

6 

Separation of mono, di, 

oligo, and polysaccharides, 

starches, and celluloses 

Pullulan, Saccharides, and Ethanol 

9163 

1. Pullulan (46.7k) 

2. Pullulan (5.3k) 

3. Raffinose 

4. Sucrose 

5. Fructose 

6. Ethanol 

0 5 

10 15 Min. 

0 2 

4 6 8 10 12 Min. 

Column: Shodex ® Protein KW-803, 8.0 x 300mm 

(Part No. 5749) 

Mobile Phase: 50mM Phosphate buffer + 0.3M NaCl, pH 7.0 




Column: Shodex ® Sugar KS-802, 8.0 x 300mm 

(Part No. 5743) 



Detector: RI 


Shodex ® Size Exclusion HPLC Columns 

Particle Size Format Packing MW Range i.d. x Length Part No. 

OHpak 8µm Analytical Q-801 50–1800 8.0 x 500mm 5737 

8µm Analytical SB-802 HQ 50–4000 8.0 x 300mm 5730 

6µm Analytical SB-802.5 HQ 50–10,000 8.0 x 300mm 5731 

6µm Analytical SB-803 HQ 50–100,000 8.0 x 300mm 5732 

10µm Analytical SB-804 HQ 100–1,000,000 8.0 x 300mm 5733 

13µm Analytical SB-805 HQ 500–4,000,000 8.0 x 300mm 5734 

13µm Analytical SB-806M HQ 100–20,000,000 8.0 x 300mm 5736 

Protein 5µm Analytical KW-802.5 50–50,000 8.0 x 300mm 5748 

5µm Analytical KW-803 50–150,000 8.0 x 300mm 5749 

7µm Analytical KW-804 100–600,000 8.0 x 300mm 5750 

Sugar 5µm Analytical KS-801 50–1000 8.0 x 300mm 5742 

5µm Analytical KS-802 50–10,000 8.0 x 300mm 5743 



17µm Analytical KS-805 100–5,000,000 8.0 x 300mm 5746 

17µm Analytical KS-806 500–50,000,000 8.0 x 300mm 5747 


Doing carbohydrate analysis? 

ELSD offers greater sensitivity than RI. See pages 5–11. 




80 


6219


Shodex ® ODP2 HP Columns 

High-Efficiency Polymer-Based 

Reversed-Phase Column 

• Column efficiency is comparable with that of silica-based 

ODS columns 

• Better retention of highly polar substances comparing to 

ODS columns 

• Long column life even with high protein content samples: 

ODP2 HP prevent protein adsorption that causes ODS 

column degradation 

• Excellent peak shape using low salt mobile phase, ideal 

for microbore 

• High pH stability 

ODP2 HP series is a polymer-based 

[poly(hydroxymethacrylate)] column for reversed-phase 

chromatography. The efficiency of ODP2 HP is improved 

over most resin-based columns, with typical theoretical plate 

number >65,000 per meter. 

ODP2 HP Columns 


ODP2 HP-4B, 5µm Analytical 4.6 x 50mm F7622001 

ODP2 HP-4D, 5µm Analytical 4.6 x 150mm F7622002 

ODP2 HP-4E, 5µm Analytical 4.6 x 250mm F7622003 

ODP2 HPG-4A, 5µm Analytical 4.6 x 10mm F6714010 

ODP2 HP-2B, 5µm Analytical 4.6 x 50mm F7622004 

ODP2 HP-2D, 5µm Analytical 4.6 x 150mm F7622005 

ODP2 HPG-2A, 5µm Analytical 4.6 x 10mm F6714011 

ODP2 HP Columns Specifications 

Packing 

Theoretical Plate Number 

(per column) 

Theoretical Plate Number 

(per meter) Particle Size pH Range i.d. x Length 

ODP2 HP-4B ≥3500 70,000 5µm 3–12 4.6 x 50mm 

ODP2 HP-4D ≥13,000 86,000 5µm 3–12 4.6 x 150mm 

ODP2 HP-4E ≥17,000 68,000 5µm 3–12 4.6 x 250mm 

ODP2 HPG-4A guard column guard column 5µm 3–12 4.6 x 10mm 

ODP2 HP-2B ≥3000 60,000 5µm 3–12 2.0 x 50mm 

ODP2 HP-2D ≥7000 46,600 5µm 3–12 2.0 x 150mm 

ODP2 HPG-2A guard column guard column 5µm 3–12 2.0 x 10mm 

hplc columns | large molecule 

Drug in Biological Fluid 

Microbore is effective for the high sensitivity analysis of drugs; however, when protein is present and enters the MS (Mass 

detector), it contaminates the MS or suppresses ionization of the sample. Often pretreatment does not remove protein 

thoroughly. Drugs in biological fluid are hard to analyze because protein co-elutes with the component of interest. The target 

drug receives ion suppression from the protein and appears as a small peak. 

ODP2 HP can separate the target drug from protein by eluting protein early. The result of barbital analysis with BSA using 

microbore is shown as below. Barbital was introduced into the MS by a switching valve after BSA was eluted, and barbital 

was detected without any influence of ion suppression. 

1,800K 

1,600K 

1,400K 

1,200K 

1,000K 

800K 

600K 

400K 

200K 

130K 

120K 

110K 

100K 

90K 

80K 

70K 

60K 

50K 

40K 

30K 

20K 

10K 

0 

Valve Switch 

1 

Barbital 

130K 

120K 

110K 

100K 

90K 

80K 

70K 

60K 

50K 

40K 

30K 

20K 

10K 

0 

Valve Switch 

1 

Barbital 

1,800K BSA 

1,600K 

1,400K 

MS 

1,200K 

1,000K 

800K 

600K 

400K 

1 

200K 

1 

0 

UV 

0 UV 

0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min. 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min. 

MS 

more info 

For other Shodex ® columns, including KW400, KF-400, SB-800, 

KF-400, KF-600, KF-800, NH2P, Ion Exchange, affinity, and sugar 

columns, visit www.discoverysciences.com. 




Waters ® Spherisorb ® Columns 

All Waters ® columns are packed by Grace to our highest QC 

standards. Spherisorb ® columns have 80Å pores and 220m 2 /g 

surface area. ODS-1 is unendcapped with a 6% carbon load, 

and ODS-2 is endcapped with a 12% carbon load. 

Spherisorb ® HPLC Columns 



Part No. 

























Spherisorb ® All-Guard Cartridges* 


ODS-1, 5µm 4.6 x 7.5mm 3 96218 

ODS-2, 5µm 4.6 x 7.5mm 3 96219 

C8, 5µm 4.6 x 7.5mm 3 96220 

C6, 5µm 4.6 x 7.5mm 3 96221 

Phenyl, 5µm 4.6 x 7.5mm 3 96226 

Cyano, 5µm 4.6 x 7.5mm 3 96225 

Amino, 5µm 4.6 x 7.5mm 3 96224 

Silica, 5µm 4.6 x 7.5mm 3 96227 

SAX, 5µm 4.6 x 7.5mm 3 96228 

SCX, 5µm 4.6 x 7.5mm 3 96217 


ea 80101 



more info 

If you like Spherisorb ® , try Grace’s lower cost Allsphere on 

page 59. 

Beckman ® Ultrasphere ® 

Columns 

• Low metal content for symmetrical peaks 

• Narrow particle size range for high efficiency 

• Maximum surface coverage for long column lifetimes 

All Ultrasphere ® columns are packed by Grace to our highest 

QC standards. Ultrasphere ® columns are highly endcapped 

to reduce silanol interactions and have a narrow particle size 

range for excellent resolving power. 

1 

Tricyclic Antidepressants 

2 3 4 5 

6 

7 

0 5 10 15 Min. 

7739 

1. Doxepin 

2. Desipramine 


4. Imipramine 



7. Clomipramine 

Column: Ultrasphere ® C18, 5µm, 4.6 x 150mm 

(Part No. 235330) 

Mobile Phase: 10mM Triethylamine, pH2.5:Acetonitrile (71:29) 



Ultrasphere ® HPLC Columns 







Prep 10 x 250mm 235328 

C18-IP, 5µm Analytical 4.6 x 150mm 235334 




Cyano, 5µm Analytical 4.6 x 150mm 244070 




Ultrasphere ® Guard Columns 

Description i.d. x Length Qty. Part No. 

C18, 5µm 4.6 x 45mm ea 243536 

C18-IP, 5µm 4.6 x 45mm ea 243534 

C8, 5µm 4.6 x 45mm ea 243532 

Cyano, 5µm 4.6 x 45mm ea 243538 

Silica, 5µm 4.6 x 45mm ea 243530 

Ultrasphere ® guard columns connect directly to the analytical column with a 

zero dead volume connector, or a small piece of tubing with finger tight fittings. 

No holder required. 

82 



ZirChrom ® Columns 

• Better pH and thermal stability than silica-based columns 

• More efficient than polymer-based columns 

• Widest range of reversed-phase selectivities 

ZirChrom Separations, Inc. produces HPLC columns based 

on 3µm zirconia (zirconium oxide) particles. These columns 

are more rugged and durable than silica-based columns 

and more efficient than polymer-based columns. ZirChrom ® 

normal-phase and reversed-phase columns can be used 

across the entire pH range (1–14) and are thermally stable to 

200°C. Additionally, zirconia’s silanol-free surface produces 

sharp, symmetrical peaks for amines without mobile phase 

modifiers. 

ZirChrom ® Packings 

Packing Functional Group Mode 

DiamondBond-C18 Octadecylsilane Reversed-Phase 

PBD Polybutadiene Reversed-Phase & 

Secondary Ionic 

PS Polystyrene Reversed-Phase 

(Low Retention) 

CARB Graphitic Carbon Reversed-Phase 

(High Retention) 

PHASE Zirconia Normal-Phase 

EZ — Reversed-Phase 

MS — Reversed-Phase 

• DiamondBond ® -C18 — ideal for microbore applications 

and acidic compounds. pH range 1–14 

• ZirChrom ® -PBD — for general purpose applications and 

basic compounds; similar to ODS for non-electrolytes. 

pH range 1–14 

• ZirChrom ® -PS — ideal for highly aqueous mobile phases; 

an alternative to ODS selectivity. pH range 1–14 

• ZirChrom ® -CARB — for diastereomers/geometric 

isomers; greatest difference in selectivity compared 

to ODS. pH range 1–14 

• ZirChrom ® -Phase — the bare zirconia particle. The 

columns are used as a normal-phase alternative to silica. 

pH range 1–14 

• ZirChrom ® -EZ — unique selectivity with mobile phase 

selectivity. pH range 1–10 

• ZirChrom ® -MS — low column bleed makes it ideal for 

microbore applications. pH range 1–10 

ZirChrom ® Specifications 

Pore Size 

300Å 

Particle Size 3µm, 5µm 

Surface Area 30m 2 /g 

pH Range 1–14 (EZ and MS 1–10) 

Max. Temperature 200°C 

EZ and MS HPLC Columns 

• Unique selectivity on a zirconia platform 

• Mobile phase flexibility, including microbore 

compatible buffers 


The deactivation of Lewis acid sites on the zirconia surface 

allows for the chromatography of Lewis base analytes using 

volatile mobile phase additives, including conventional 

microbore compatible buffers such as acetate and formate. 

ZirChrom ® HPLC Columns 


DiamondBond ® , 3µm Microbore 2.1 x 100mm DB01-1021 

Microbore 2.1 x 150mm DB01-1521 

Analytical 4.6 x 50mm DB01-0546 



PBD, 3µm Microbore 2.1 x 100mm ZR03-1021 

Microbore 2.1 x 150mm ZR03-1521 

Analytical 4.6 x 50mm ZR03-0546 



PS, 3µm Microbore 2.1 x 100mm ZR09-1021 





CARB, 3µm Microbore 2.1 x 100mm ZR01-1021 




PHASE, 3µm Microbore 2.1 x 100mm ZR02-1021 




EZ, 3µm Microbore 2.1 x 50mm EZ01-0521 

Microbore 2.1 x 100mm EZ01-1021 



Analytical 4.6 x 50mm EZ01-0546 




EZ, 5µm Microbore 2.1 x 50mm EZ01-05215 








MS, 3µm Microbore 2.1 x 50mm MS01-0521 

Microbore 2.1 x 100mm MS01-1021 



Analytical 4.6 x 50mm MS01-0546 




MS, 5µm Microbore 2.1 x 50mm MS01-05215 











Vydac ® Large Molecule Columns 

Vydac ® has always been a trusted name in bioseparations, now, with technology acquired by Grace over the past few years, 

we have expanded this expertise further. New Grace ® large molecule columns range from nano, capillary to micro, analytical 

to preparative columns. 


Separate biomolecules from small peptides to large intact proteins with the Vydac ® family of products which includes reversedphase, 

normal-phase, ion-exchange, and affinity phases. Our extensive applications library offers solid method development 

guidance, and our technical experts provide insight to even the most unusual separation challenges. Whether your primary 

analysis consideration is speed, MS compatibility, resolution, or recovery, we have a solution. 

Column Selection for Polypeptides 

The reversed-phase column for a polypeptide separation should be selected based on the hydrophobicity of the polypeptide 

being chromatographed and molecular weight as a secondary consideration. 

Molecular Weight 

1000 

Increasing hydrophilicity 

10,000 

C 18 

= 

GraceAlpha 

C 18 

= 218TP, 238TP, 218MS 

C 8 

= 208TP, 208MS 

C 4 

= 214TP, 214MS 

Increasing hydrophobicity 

100,000 

Diphenyl = 219TP 

Phase 

Simple Enzymatic 

Digests 

(12 proteins) 

Biomolecules 

0-5K MW 

Biomolecules 

5-10K MW 

Biomolecules 

>10K MW 

Un-denatured, 

Intact Proteins 

Antibodies 

Oligonucleotides 

Oligosaccharides 

Comments 

218MS (C18) 

See page 85–87 

Polymeric bonding highest hydrophic 

interaction and unique geometric selectivity 

238MS (C18) 


Monomeric bonding offers increased peptide 

interaction and generally yields higher peak 

counts. Different selectivity compared to 

218MS. 

208MS (C8 


Lower hydrophobicity is better for larger 

molecules 

214MS (C4) 


Ideal for hydrophobic proteins, or when 

minimal organic is desired 

219MS (Diphenyl) 


Lowest capacity, highly selective for proteins 

with aromatic sidechains 

Everest ® C18 


Maximum surface coverage for highest 

resolution of complex samples 

ProZap 


Sub 2µm, in Expedite format optimized for 

fast analysis 

218TP (C18) 


First generation media with extensive 

applications library 

214TP (C4) 


First generation media with extensive 

applications library 


Looking for large molecule prep columns? 


84 



Vydac ® MS Introduction 

New Generation Columns with Unsurpassed Resolution, Sensitivity, 

and Recovery 

• Unique selectivity reveals peaks otherwise masked by other C18 columns 

• Excellent peak shape with little or no TFA 

• High protein recoveries make scale-up easy 

Vydac ® MS columns are the latest development in the ongoing effort to provide the best reversedphase 

HPLC columns for biomolecule. A surface treatment and propriatary bonding process gives 

Vydac ® MS columns a unique selective not found anywhere else. A variety of reversed phases makes 

this product line suitable for small peptides to large intact, undenatured proteins. 

MS Specifications 


208MS C8 Silica Spheroidal 5µm 300Å 70m 2 /g 5% Polymeric Yes L7 

214MS C4 Silica Spheroidal 5µm 300Å 70–110m 2 /g 3% Polymeric Yes L26 

218MS C18 Silica Spheroidal 3, 5, 10, 10–15µm 300Å 60–110m 2 /g 8% Polymeric Yes L1 

238MS C18 Silica Spheroidal 5µm 300Å 70m 2 /g 4% Monomeric Yes L1 

219MS Di-Phe Silica Spheroidal 5µm 300Å 70m 2 /g 4% Polymeric Yes — 

Unique Selectivity 

A sample of bovine fetuin, a 36kD glycoprotein, was digested 

with trypsin. Some of the sample components interfere with 

the peptide separation on the Competitor 1 and Competitor 2 

columns and appear as a chromatographic “hump” with peaks 

riding on top. The unique selectivity of Vydac ® MS columns 

solves these separation problems 

1 

2 4 

4 

1 

4 

3 

2 

4 

1 3 

4 

2 

1 

3 

2 

1 2 

10 20 30 40 50 60 65 Min. 

1. 18.8 kD Protein 

2. Non-myristoylated p14 

3. Myristoylated p14 

4. Triton ® X (Surfactant) 

Vydac ® MS C4 Column 





Columns: Vydac ® MS C4 214MS54, Competitor 1 C4, 

Competitor 2 C3, Competitor 3 C4, and 

Competitor 4 C4 (all 300Å, 5µm, except 4µm for 

Competitor 1, 4.6 x 250mm) 

Flow Rate: 

Mobile Phase: 

Transmembrane Protein p14 Columns 

1.0mL/min 

A: 0.1% v/v TFA in H2O 

B: 0.085% v/v TFA in Acetonitrile 

Gradient Vydac ® , Comp. 1, and Comp. 2: 

Time: 0 20 25 45 

%B: 20 60 80 80 

Gradient Comp. 3 and Comp. 4: 

Time: 0 20 25 45 50 80 

%B: 20 60 80 80 90 90 


Column Temp: Ambient, 25°C 


Unsurpassed Resolution and Peak Symmetry 

Vydac ® MS C4 columns provide the overall best performance 

for hGH and desamido hGH analysis. Competitive columns 

show significant, undesirable interaction of hGH with the 

stationary phase. 

Human Growth Hormone (hGH) Assay Columns 

1. Desamido HGH 

2. HGH 

2 

2 

1 2 

Vydac ® MS C4 Column 

1 


1 


1 2 


0 20 40 60 Min. 

Columns: All 300Å, 5µm, 4.6 x 250mm 

Mobile Phase: Isocratic, 71% 50mM Tris, pH 7.5, 29% n-propanol 




Injection Vol: 20µL of a 1mg/mL preparation 

more info 

For additional protein and peptide applications, see application 

section pages 429–441. 





7300 


Reptilian reovirus RRV p14 sample courtesy of Drs. Roberto J. de Antueno and Roy 

Duncan, Dalhousie University, Halifax, Nova Scotia. 





Excellent Peak Shape with Little or No TFA 

It is common practice for protein and peptide separations to 

include an acidic modifier such as TFA in the mobile phase. 

TFA masks basic entities, reducing mixed-mode retention, 

and improving peak symmetry. TFA also changes retention 

and selectivity for different analytes, and its concentration 

can be adjusted to optimize a separation. Unfortunately 

TFA is UV absorbent and contributes background at low 

UV wavelengths. Also It is especially problematic with 

electrospray MS where it interferes with ion generation, 

called “quenching”, and reduces MS sensitivity. 

0.6 AU 

0.4 

0.2 

0 

0 

Columns: 

0.1% TFA 

Flow Rate: 

Mobile Phase: 

Gradient: 

Detector: 

Peptides on 218MS C18 Reversed-Phase 

0.05% TFA 

0.02% TFA 

0.01% TFA 

1 

1 

1 

1 

3 

2 

2 

2 

3 

2 

4 

3 

1. Neurotensin 1-8 (fragment) 

2. Oxytocin 


4. Neurotensin 

4 

10 20 30 Min. 

Vydac ® C18, 5µm, 4.6 x 250mm 

(Part No. 218MS54) 

1.5mL/min 

A: 5% Acetonitrile in Water with TFA as indicated (v/v) 

B: 95% Acetonitrile in Water with TFA as in A 

Linear from 0 to 20% B over 20min, then to 

100% B in 5min 

UV at 220nm 

3 

4 

4 

Hydrophobic Proteins 

Transmembrane proteins are hydrophobic proteins 

which bind to cell membranes and are particularly difficult to 

separate. Vydac ® MS columns provide excellent selectivity 

and peak shape for these molecules. In this case, a 

hydrophobic transmembrane protein was separated from 

a synthetic myristoylated derivative and other cellular 

components. 

Tryptic Digest of Fetuin on 

300Å C18 Columns 

Vydac ® MS C18 

Column 

Competitor 1 C18 

Column 


Column 

0 20 40 60 80 Min. 

Columns: Vydac ® MS C18 238MS5415, Competitor 1 C18, and 

Competitor 2 C18 (all 300Å, 5µm, 4.6 x 150mm) 


Mobile Phase: A: 0.1% v/v TFA in H2O 

B: 0.085% v/v TFA acid in Acetonitrile 


%B: 4 4 40 90 90 



Injection Vol: 3µL of a 6 µg/µL digest (180 µg total peptide load) 

The Vydac ® column provides better separation and recovery for a 

highly hydrophobic membrane protein (RRV p14) and its fatty acid 

modified (myristolyated) form, a component of a potentially new 

vaccine delivery system. 

High Protein Recovery for Higher Sensitive, 

Ideal for Preparative Chromatography 

Surface chemistry reduces adsorption of proteins for higher 

recoveries and also increases mass loading. 





% Recovery at Low Protein Load: Vydac ® MS C4 vs. 

2 Competitors 

100 

80 

Loading Amount: 

1µg of Cytochrome C 

% Recovery 

60 

40 

20 

Vydac ® MS 

Competitor 1 

Competitor 2 

0 

Vydac ® MS C4 column provides more than 20% more loading of 

Cytochrome C. 

86 




Vydac ® MS Analytical Columns 

Columns 



214 MS C4 

5µm 1.0mm 214MS5105 214MS5110 214MS5115 214MS51 214GK51MS 214GD51MS 

2.1mm 214MS5205 214MS5210 214MS5215 214MS52 214GK52MS 214GD52MS 

3.2mm — — — 214MS53 214GK54MS 214GD54MS 


208 MS C8 



3.2mm — — — 208MS53 208GK54MS 208GD54MS 

4.6 mm 208MS5405 208MS5410 208MS5415 208MS54 208GK54MS 208GD54MS 

218 MS Polymeric C18 



3.2mm — — — 218MS53 218GK54MS 218GD54MS 


238 MS Monomeric C18 



3.2mm — — — 238MS53 238GK54MS 238GD54MS 


219 MS Diphenyl 



3.2mm — — — 219MS53 219GK54MS 219GD54MS 


1 

A guard kit includes a holder and one guard cartridge; 2 Guard cartirdge units include two guard cartridges. 


Vydac ® MS Nano and Capillary Columns 

i.d. 50mm 100mm 150mm 250mm 

214 MS C4 

5µm 75µm 214MS5.07505 214MS5.07510 214MS5.07515 214MS5.07525 

150µm 214MS5.1505 214MS5.1510 214MS5.1515 214MS5.1525 

300µm 214MS5.305 214MS5.310 214MS5.315 214MS5.325 

500µm 214MS5.505 214MS5.510 214MS5.515 214MS5.525 

208 MS C8 

5µm 75µm 208MS5.07505 208MS5.07510 208MS5.07515 208MS5.07525 

150µm 208MS5.1505 208MS5.1510 208MS5.1515 208MS5.1525 

300µm 208MS5.305 208MS5.310 208MS5.315 208MS5.325 

500µm 208MS5.505 208MS5.510 208MS5.515 208MS5.525 

218 MS Polymeric C18 

3µm 75µm 218MS3.07505 218MS3.07510 218MS3.07515 218MS3.07525 

150µm 218MS3.1505 218MS3.1510 218MS3.1515 218MS3.1525 

300µm 218MS3.305 218MS3.310 218MS3.315 218MS3.325 

500µm 218MS3.505 218MS3.510 218MS3.515 218MS3.525 

5µm 75µm 218MS5.07505 218MS5.07510 218MS5.07515 218MS5.07525 

150µm 218MS5.1505 218MS5.1510 218MS5.1515 218MS5.1525 

300µm 218MS5.305 218MS5.310 218MS5.315 218MS5.325 

500µm 218MS5.505 218MS5.510 218MS5.515 218MS5.525 

238 MS Monomeric C18 

5µm 75µm 238MS5.07505 238MS5.07510 238MS5.07515 238MS5.07525 

150µm 238MS5.1505 238MS5.1510 238MS5.1515 238MS5.1525 

300µm 238MS5.305 238MS5.310 238MS5.315 238MS5.325 

500µm 238MS5.505 238MS5.510 238MS5.515 238MS5.525 

219 MS Diphenyl 

5µm 75µm 219MS5.07505 219MS5.07510 219MS5.07515 219MS5.07525 

150µm 219MS5.1505 219MS5.1510 219MS5.1515 219MS5.1525 

300µm 219MS5.305 219MS5.310 219MS5.315 219MS5.325 

500µm 219MS5.505 219MS5.510 219MS5.515 219MS5.525 

Vydac ® MS Guard Cartridges 


C18 Polymeric, 5µm* 


0.150 x 10mm ea 218MS5C0115 

0.300 x 10mm ea 218MS5C0130 

1.0 x 10mm 2 218GD51MS 

C4, 5µm* Capillary Guard** 0.150 x 10mm ea 214MS5C0115 

0.300 x 10mm ea 214MS5C0130 

1.0 x 10mm 2 214GD51MS 



Vydac ® MS Guard Cartridges (continued) 

Packing Qty. Part No. 




Guard Holder for 1mm Guards ea GCH1 




Vydac ® Everest ® Columns Introduction 

High Resolution for Complex Peptide Samples 

• High resolution of complex peptide digests 

• Unique selectivity for both hydrophilic and hydrophobic peptides 

• Exceptional microbore sensitivity with little or no TFA in the mobile phase 

Everest ® columns provide exceptionally high resolution for complex peptide digest separations. 

Unique selectivity and sensitivity are the result of new bonding technology that improves C18 

surface coverage and deactivates residual silanols. Previously, the best 300Å C18 chemistries have 

had carbon coverage in the 2.8 to 3.6µmol/m 2 range. Everest ® C18 coverage is in excess of 4µmol/m 2 

and approximates the theoretical limit based on surface area. This increased shielding of the base silica 

increases column life and reduces the amount of TFA required to shield the silica. 

Everest ® Specifications 


238EV C18 Silica Spheroidal 5, 10, 10–15, 15–20µm 300Å 70–110m 2 /g 6% Monomeric Yes L1 

Tryptic Digest of Bovine Serum Albumin 

Everest ® Column 

110 peaks 

Commercial Column C 

94 peaks 

Commercial Column D 

96 peaks 

Intensity (x 10 6 cps) 

Base Peak Chromatogram of Tryptic Digest of BSA 

7 

6 

5 

4 

3 

2 

1 

7189 

0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 Min. 

Column: Vydac ® Everest ® C18, 300Å, 5µm, 4.6 x 150mm 

Commercial 300å c18 columns 4.6 x 150mm 

(Part No. 238EV5415) 

Mobile Phase: A: 0.1% TFA in Water 

B: 0.085% TFA in Acetonitrile 


%B: 4 4 40 90 90 



Everest ® columns outperform competitor columns at higher peptide 

loads, by providing higher-resolution separations (average of 17% 

higher peak counts than competitor columns tested). 

0 

0 5 15 25 35 45 55 65 75 85 95 Min. 

Column: Vydac ® Everest ® C18, 300Å, 5µm, 300µm x 150mm; 

(Part No. 238EV5.315) 

Mobile phase: A: 0.1% v/v Formic Acid in Water 

B: 0.1% v/v Formic Acid in Acetonitrile 


%B: 4 4 40 90 90 

Flow Rate: 5µL/min 


Detector: MS + ESI 

The Everest ® column performs exceptionally well with no TFA in the 

mobile phase, ensuring excellent microbore sensitivity. 

Everest ® 300Å C18 

Columns 



Everest ® Analytical Columns 238EV C18 

5µm 1.0mm 3 238EV5105 238EV5110 238EV5115 238EV51 238GK51EV 238GD51EV 

2.1mm 3 238EV5205 238EV5210 238EV5215 238EV52 238GK52EV 238GD52EV 

4.6mm 3 238EV5405 238EV5410 238EV5415 238EV54 238GK54EV 238GD54EV 

Everest ® Capillary Columns 238ZU C18 

5µm 75µm 238EV5.07505 238EV5.07510 238EV5.07515 238EV5.07525 — — 

150µm 238EV5.1505 238EV5.1510 238EV5.1515 238EV5.1525 — — 

300µm 238EV5.305 238EV5.310 238EV5.315 238EV5.325 — — 

500µm 238EV5.505 238EV5.510 238EV5.515 238EV5.525 — — 

1 

A guard kit includes a holder and one guard cartridge. 2 Guard cartirdge units include two guard cartridges. 3 Titamium frits are standard in column diameters 4.6mm and smaller. 

Everest ® Guard Cartridges 


C18, 5µm* Capillary Guard** 0.150 x 10mm ea 238EV5C0115 

0.300 x 10mm ea 238EV5C0130 

1.0 x 10mm 2 238GD51EV 



Everest ® Guard Cartridges (continued) 

Packing Qty. Part No. 




Guard Holder for 1mm Guards ea GCH1 

88 



Vydac ® Everest ® Columns 

Tryptic Digest of Cytochrome C 

Columns: 

Flow Rate: 

Mobile Phase: 

Everest ® 238EV5415 and 4 competitor 

300Å reversed-phase columns, all C18, 

5µm, 4.6 x 150mm 

1.0 mL/min 

A: 0.1% v/v TFA in water 

B: 0.085% v/v TFA in ACN 


%B: 4 40 90 90 

hold 90% B for 10 min 

Detector: 

UV at 215nm 


4 3 

Competitor Column B 

3 

3 

Competitor Column D 

3 1 

Competitor Column E 

3 

2 

Competitor Column F 

3 2 

Number of Well-Resolved 

Peptide in Highlighted Areas 

7 

6 

4 

5 

5 


0 5 10 15 20 25 30 35 40 45 50 55 60 65 Min. 

For a tryptic digest of Cytochrome C, an Everest ® column offers unique selectivity which allows the best separation of a group of hydrophilic 

and hydrophobic peptides. To assess resolution, chromatograms of tryptic digests of several proteins on an Everest ® column and several other 

commercial 300Å C18 reversed-phase columns were compared. Peak counts were based on detection by automated chromatography software 

with parameters set identically for all columns. In the analyses of a tryptic digest of cytochrome c, specific regions of the chromatogram were 

examined for the number of peaks resolved. The Everest ® column demonstrated unique selectivity for both hydrophilic and hydrophobic peptides. 

Tryptic Digest of Fetuin (a Glycoprotein) 


98 peaks 

Competitor Column A 

97 peaks 

Columns: Everest ® 238EV5415 and 4 competitor 

300Å reversed-phase columns, all C18, 

5µm, 4.6 x 150mm 

Flow Rate: 1.0 mL/min 

Mobile Phase: A: 0.1% v/v TFA in water 

B: 0.085% v/v TFA in ACN 


%B: 4 4 40 90 90 


Competitor Column B 

90 peaks 

Competitor Column C 

95 peaks 

Competitor Column D 

89 peaks 

0 10 20 30 40 50 60 70 80 Min. 

A tryptic digest of fetuin injected at high load on an Everest ® column provided higher peak counts compared to four commercial columns. Peak 

numbers shown are the average of three separations on each column. 








Vydac ® TP Columns 

Industry Standard for Polypeptide Separations 

• Referred in over 9000 patents, Vydac ® 300Å TP is the industry-standard, for peptide, 

protein, and large molecule separations 

• Polymeric bonded phases have exceptionally long column lifetime and negligible 

phase leaching 

• Extensive applications library based on over two decade’s experience 

Vydac ® TP reversed-phase material consists of aliphatic groups bonded to the surface of 300Å pore diameter silica. The large 

pores of the 300Å TP silica give polypeptide molecules complete access to the interior of the silica pores. The unique process by 

which we manufacture Vydac ® TP silica results in high-purity, synthetic silica with carefully controlled characteristics. Vydac ® TP 

silica is the standard that has defined large pore HPLC silica for polypeptide separations for nearly two decades. 



101TP Sil Silica Spheroidal 5, 10, 10–15, 15–20µm 300Å 70–110m 2 /g — unbonded — L3 

201TP C18 Silica Spheroidal 5, 7, 10, 10–15, 15–20µm 300Å 70–90m 2 /g 8% Polymeric No L1 


208TP C8 Silica Spheroidal 3, 5, 7, 10, 10–15, 15–20µm 300Å 60–110m 2 /g 5% Polymeric Yes L7 



219TP Di-Phe Silica Spheroidal 3, 5, 7, 10, 10–15, 15–20µm 300Å 60–110m 2 /g 4% Polymeric Yes — 

238TP C18 Silica Spheroidal 3, 5, 7, 10, 10–15, 15–20µm 300Å 60–110m 2 /g 4% Monomeric Yes L1 

Vydac ® 218TP C18 Columns 

Vydac ® 218TP is a polymerically bonded endcapped 

n-octadecyl reversed-phase based on 300Å TP silica. 

Applications 

Vydac ® 218TP reversed-phase columns are recommended for 

the separation of: 

• Small polypeptides less than 4000–5000 MW 

• Enzymatic digest fragments 

• Natural and synthetic peptides 

• Multi-ring compounds 

Specific examples include: 

• Tryptic digests 

• S. aureus V8 digests 

• Synthetic peptides 

• Natural peptides 

• Peptide studies 

Standard 

Human Apolipoproteins 

7 

4 6 5 

1 2 3 

0 5 10 15 20 25 30 35 Min. 

Column: Vydac ® 218TP54, C18, 5µm, 4.6 x 250mm 



Mobile Phase: A: 25% ACN/0.1% TFA in Water 

B: 58% ACN/0.1% TFA in Water 

Gradient: 0–100% B in 33min 

8 

11 

10 

9 

7110 

1. apoC-IIIa 

2. apoC-IIIb 

3. apoC-IIa 

4. apoC-IIIc 

5. apoC-I 

6. apoC-IIb 

7. apoA-Ia 

8. apoA-Ib 

9. apoA-IIc 

10. apoA-IIa 

11. apoA-IIb 

The standard was 

human insulin 

Vydac ® 208TP C8 Reversed-Phase 

Vydac ® 208TP is a polymerically bonded endcapped n-octyl 

reversed-phase based on 300Å TP silica. 

Separation of a Tryptic Digest on 208TP54 

Reversed-Phase Column 

Applications 

Vydac ® 208TP reversed-phase columns are recommended for 

the separation of: 

• Polypeptides up to 10,000–20,000 MW 



0 10 20 30 40 50 60 Min. 

Column: 

Flow Rate: 

Gradient: 

Vydac ® 208TP54, C8, 5µm, 4.6 x 250mm 

1.0mL/min 

0–30% ACN with 0.1% aqueous TFA over 60min 

90 



Vydac ® 214TP C4 

Vydac ® 214TP is a polymerically bonded endcapped 

n-butyl reversed-phase based on 300Å TP silica. 214ATP is 

a less extensively endcapped C4 that has been found more 

suitable for resolution of degradation products in analysis of 

biosynthetic human growth hormone. 

Applications 

• Glycoproteins 

• Hemoglobin variants 

• Histones 

• Human growth hormone 

• Insulin variants 

• Membrane proteins 

Vydac ® 219TP Diphenyl Reversed-Phase 

Vydac ® 219TP is a polymerically bonded endcapped diphenyl 

reversed-phase based on 300Å TP silica. It combines 

moderate retentivity with unique selectivity. 

Applications 

• Polypeptides with aromatic side chains 

• Large, hydrophobic proteins 

• Membrane-spanning peptides 

• Lipid peptides 

• Fusion proteins from inclusion bodies 

1 

0 5 10 15 20 25 Min. 

Column: 

Gradient: 

Insulin Variants 

2 

5 6 

4 

3 

7 

8 

Vydac ® C4, 5µm, 4.6 x 250mm (Part No. 214TP54) 

27–30% ACN with 0.1% TFA over 25min 

From J. Rivier and R. McClintock, J. Chrom. 268, 112-119 (1983). 

1. Chicken 

2. Bovine 

3. Ovine 

4. Rabbit 

5. Human 

6. Porcine 

7. Rat I 

8. Rat II 

Comparison of Polypeptide Separations on C18, C4, 

and Diphenyl Reversed-Phase Columns 

218TP 

(C18) 

214TP 

(C4) 

219TP 

(diphenyl) 

1 

1 

1 

2 

2 

2 

1. H 

2. TH6 


0 5 10 15 20 25 30 Min. 

Columns: 

Gradient: 

Sample: 

Vydac ® 218TP5415 (C18), 214TP5415 (C4) and 

219TP5415 (diphenyl), all 5µm, 4.6 x 150mm 

10–90% ACN with 0.1% TFA over 30min 

18-residue helical peptide (1) and six-helix template 

assembled synthetic protein (2) 

Vydac ® 238TP C18 Reversed-Phase 

Vydac ® 238TP is a monomerically bonded endcapped 

n-octadecyl reversed-phase based on 300Å TP silica. 

Applications 

Monomerically bonded C18 provides an alternative to 

218TP polymeric C18 with subtle differences in selectivity. 

The combination of these adsorbents can reveal analytes that 

may be hidden on a single C18 column. 

more info 

For more protein and peptide applications, see the application 

section pages 429–441. 





13,14 

218TP54 

polymeric C18 811,12 

9 

10 1516 

24 

1 356 

7 

238TP54 

monomeric C18 

4,5 

2 

1 6 

3 

Columns: 

Flow Rate: 

Gradient: 

Detector: 

Synthetic Peptides 

8 14 

911 

13 1516 

7 10 12 

0 5 10 15 20 25 30 Min. 

1. GY 

2. VYV 

3. Neurotensin fragment 

1-8 (pELYENKPR) 

4. Ac-RGGGGLGLGK-NH2 

5. RGAGGLGLGK-NH2 

6. Ac-RGAGGLGLGK-NH2 

7. Ac-RGVGGLGLGK-NH2 

8. Oxytocin (CYIQNCPLG-NH2) 

9. Met Enkephalin (YGGFM) 

10. Bradykinin (RPPGFSPFR) 

11. Ac-RGVVGLGLGK-NH2 

12. Neurotensin Fragment 

8-13 (RRPYIL) 

13. Angiotensin II (DRVYIHPF) 

14. Leu Enkephalin (YGGFL) 


(pELYENKPRRPYIL) 

16. Angiotensin I 

(DRVYIHPFHL) 

Vydac ® 218TP54 and 238TP54, both C18, 5µm, 

4.6 x 250mm 

1.0mL/min 

10–40% ACN with 0.1% TFA (w/v) over 30min 

UV at 220nm 





Vydac ® TP Analytical Columns 

Columns 



218TP C18 

3µm 4.6mm 218TP3405 218TP3410 — — 218GK34 218GD34 

5µm 1.0mm 218TP5105 218TP5110 218TP5115 218TP51 218GK51 218GD51 

2.1mm 218TP5205 218TP5210 218TP5215 218TP52 218GK52 218GD52 



208TP C8 

3µm 4.6mm 208TP3405 208TP3410 — — 208GK34 208GD34 





214TP C4 

3µm 4.6mm 214TP3405 214TP3410 — — 214GK34 214GD34 





214ATP C4 Columns 

5µm 2.1mm — — — 214ATP52 — — 

4.6mm — — — 214ATP54 — — 

219TP Diphenyl 

3µm 4.6mm 219TP3405 219TP3410 — — 219GK34 219GD34 





238TP C18 

3µm 4.6mm 238TP3405 238TP3410 — — 238GK34 238GD34 





NOTE: Additional column diameters and lengths are available on request. Please contact Grace Davison Discovery Sciences to discuss your requirements. 

1 

A guard kit includes a holder and one guard cartridge; 2 Guard cartirdge units include two guard cartridges. 


For prep Vydac ® TP Columns, see our prep section 

pages 158–160. 



prefilters? See page 111. 







6673 






Need HPLC tubing? 


5535 

92 



Vydac ® ProZap C18 

Ultra Fast Protein and Peptide Separations 

3 4 

• 10 times faster bioseparations than traditional column formats 1 2 5 

• Ultra-high efficiency 1.5μm packing 

• Fast protein and peptide separations with conventional HPLC systems 

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min. 

ProZap 1.5μm, 500Å packings are ideal for fast bioseparations in life science applications. The 

combination of ProZap packings and short, 10mm Expedite column hardware delivers not only 

faster sample throughput, but also low back pressures suitable for conventional LC systems. 

Optimization of Gradient Parameters for Fast Protein Analysis 

4 

Short ProZap columns are the perfect tool for fast reversed-phase protein separations. Under gradient conditions, longer 

6 

columns only increase run time and do not increase resolution to improve the separation. 3 Proteins adsorb at the head of the 

column and then desorb once the critical mobile phase concentration is reached. Since the proteins 7 do not interact with the full 

length of the packed bed, short columns are sufficient for full resolution. Therefore, proteins of broad molecular weight ranges 

1 2 5 

can be separated in less than one minute by combining short columns, higher flow rates, and fast, modified gradients. For best 

results high-pressure mixing should be used with fast gradients. 

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min. 

ProZap C18 Specifications 


C18 ProZap Silica Spherical 1.5µm 500Å 59m 2 /g 3% Monomeric Yes L1 

6 

7 

7279 


Proteins 

3 4 6 

7 

1 2 5 

1. Ribonuclease (37ng) 

2. Bovine Insulin (37ng) 

3. Cytochrome C (37ng) 

4. Lysozyme (4ng) 

5. Bovine Serum Albumin (37ng) 

6. Carbonic Anhydrase (37ng) 

7. Ovalbumin (37ng) 

1 

2 

3 

4 

5 

6 

7 

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min. 


Backpressure: 185bar = 2680psig 

Gradient: Time: 0 1.0 1.1 1.2 

%B: 23 75 75 23 

3 

1 2 

4 

5 

6 

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min. 




%B: 23 75 75 23 

4 

6 

3 

more info 

7 

For more information 2 about the Expedite hardware format, 

see page 31. 1 

5 

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min. 

7 

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min. 




%B: 23 75 75 23 

Column: ProZap C18, 2.1 x 10mm, C18, 1.5µm, 500Å 

HPLC System: High-pressure gradient, binary pump 


B: 0.085% TFA in Acetonitrile 



Injection Amt: 37ng each protein, except 4ng for lysozyme 


Seven proteins were tested on a 10mm ProZap column. By increasing flow rate and reducing gradient time, total run time is reduced from 

4.5 minutes to 1.2 minutes. 





6113 




Vydac ® ProZap Columns 

2 

1 

2 

Antibodies in Sheep Serum 

ProZap vs. Ion Exchange 

0 1 2 3 4 5 6 Min. 

3 

4 

5 

Peptides 

1. Albumin 

2. IgG 




B: 0.085% TFA in 90:10 n-propanol:water 

Gradient: Time: 0.0 6.0 6.5 7.0 

%B: 5 75 75 5 






1. GY (238 Da) 

2. VYV (379 Da) 

3. Met Enkephalin (YGGFM, 573 Da) 

4. Leu Enkephalin (YGGFL, 555 Da) 

5. Angiotensin II (DRVYIHPF, 1045 Da) 

Separation of Antibodies in Sheep Serum 

1 

0 3 5 7 9 11 13 15 Min. 

2 

1. IgG 

2. Albumin 

Column: DEAE-type anion exchange, 7.5 x 50mm) 

Mobile Phase: 10mM HEPES/TEA, pH 8.0 

Gradient: 0 to 0.5 M NaCl in 20min 

Vydac ® ProZAP columns separate intact IgG antibodies (150 kDa) 

rapidly. Other traditional HPLC methods, such as ion exchange take 

longer and typically involve the use of non-volatile buffers. 

Synthetic Peptides —10 Injections 

1 

2 3 4 5 

1. Contaminant peptides/impurities 

2. RGAGGLGLGK-amide (883 Da) 

3. Ac-RGAGGLGLGK-amide (926 Da) 

4. Ac-RGVGGLGLGK-amide (954 Da) 

5. Ac-RGVVGLGLGK-amide (996 Da) 

1 

0 0.2 0.4 0.6 0.8 1.0 1.2 Min. 




B: 0.085% TFA in 80:20 Acetonitrile:Water 

Gradient: Time: 0.0 0.1 0.7 1.1 1.2 

%B: 4 15 20 50 4 




Injection Amt: 600ng each, except 200ng for GY 



Peptides under one minute. 

0 0.2 0.4 0.6 0.8 1.0 Min. 




B: 0.085% TFA in 80:20 Acetonitrile:Water 

Gradient: Time: 0.0 0.1 0.7 1.1 1.2 

%B: 4 15 20 50 4 




Injection Amt: 500ng each 



Reproducibly separate synthetic peptides can be in one minute. 

ProZap (1.5µm) 

Dimension 2.1 x 10mm 2.1 x 20mm 4.6 x 10mm 4.6 x 20mm 7 x 33mm 

Part No. 35585 35587 35586 35588 35589 

94 



Vydac ® Venture ® A 

Protein A Affinity Columns for Antibody 

Purification 

• High binding capacity for polyclonal and monoclonal 

antibodies 

• No non-specific binding—patented ICEtech (Inert 

Coating Enhancement Technology, U.S. Patent No. 

6,802,966) completely passivates the silica surface to 

ensure highly purified antibodies 

• Long lifetime—proven over 300 runs 

• Standard HPLC column format uses biocompatible PEEK 

hardware for use with HPLC or FPLC instruments 

The Venture ® A column is the first affinity column to take 

advantage of silica’s rigid porous structure, providing 

increased productivity over other supports. Grace’s 

patented technology, ICEtech (inert coating enhancement 

technology), eliminates non-specific binding on the silica 

surface enabling the breakthrough use of silica for affinity 

separations. 

Venture ® A columns use a recombinant protein A ligand for 

binding antibodies. They are designed to perform both as an 

analytical tool for fast, accurate measurement of antibody 

titers and as a quick and easy way to purify antibodies from 

cell culture supernate, serum, or other feedstocks. 

High Recovery: Experience Up to a 10% 

Improvement in Recovery Compared to Other 

Commercially Available Columns, Increasing Your 

Yield and Measurement Accuracy 

20mM Phosphate and 0.15M NaCl Buffer pH 7.4 was loaded 

on each column up to 90% of the total capacity. After loading 

the columns were washed with phosphate buffer and the IgG 

eluted with citrate buffer pH 3.2. 

% Recovery 

90 

88 

86 

84 

82 

80 

78 

76 

Venture ® A Column A Column B Column C 

IgG Purification from Bovine Serum 

2 

1. Serum Load Passthrough 

2. IgG 


Venture ® A Characteristics 

Composition: 

Wide-porous spherical silica treated with 

ICEtech surface passivation technology 

Particle Size: 

15–20µm 

Ligand: 

Recombinant protein A 

pH Range: 

pH 1 to 8 (long term), pH 1 to 9 (short term) 

Pressure: Maximum operating pressure = 

3000psig (200bar) 

Recommended 500cm/h 

Mobile-Phase Velocity: 

Mobile-Phase 

150–5000cm/h 

Velocity Range: 

Delivery Conditions: 20% ethanol 

Storage: 

At +4 to +8°C in 20% ethanol 

Chemical Stability: Stable in all aqueous buffers with pH 1 to 8 

commonly used in protein A chromatography 

Static Capacity of Venture ® A Affinity Columns 

Polyclonal 

Species 

Capacity (mg/mL) 

human 40 

bovine 23 

mouse 38 

rabbit 37 

sheep 13 

Monoclonal 

Subclass 

Capacity (mg/mL) 

IgG1 Kappa 15 

IgG2a 29 

IgG2b 23 

1 

0 5 15 25 35 45 Min. 

Column: 

Flow Rate: 

Mobile Phase: 

Gradient: 

Detector: 

Venture ® A RAVE1520405 (Recombinant 

Protein A, 15 – 20µm, 4.6 x 50mm) 

1.38mL/min (linear velocity = 500cm/hr) 

A: 20mM phosphate, pH 7.4, 150mM NaCl 

B: 0.1M sodium citrate, pH 3.2 

Equilibrate with A for 3min, load sample for 9min, 

wash with A for 12min, elute with B for 3min, 

re-equilibrate with A for 6min 

UV at 280nm 

Venture ® A Affinity Columns 

Particle Size i.d. 50mm 100mm 

15 –20µm 2mm RAVE1520205 — 

4.6mm RAVE1520405 RAVE1520410 

7.5mm — RAVE1520710 

NOTE: Additional column diameters and lengths are available on request. Please 

contact Grace Davison Discovery Sciences to discuss your requirements. 


Venture ® Immunoaffinity columns are also developed for small molecules, which have been fully validated. Applications areas include: 

Food (Aflatoxin, Lactoferrin, Vitamin B12, Testosterone, Nortestosterone), Endocrine Disruptors (17α-Ethynyl Estradiol, 17β-Estradiol, 

Estrone, Bisphenol A) and Pollutants (Chlorophenoxy Acetic Acid Herbicides, Phenylurea Herbicides, Organophosphorus Pesticides, 

Vinclozolin Fungicide). For details, visit www.discoverysciences.com. 




Alltech ® ProSphere HP 300Å Columns 

• High recoveries at low sample concentrations and 

low TFA concentrations 

• Use for protein, peptides, sequencing, nucleic acid 

fragments 

• pH range 2–7.5 

These columns produce sharp symmetrical peaks even 

for basic biomolecules. The 300Å pores allow access of 

large proteins while still maintaining high resolution of 

small peptides. 

A C4 Competitive Comparison 

1 

2 

3 

0 5 10 15 20 25 30 Min. 

4 

5 

10466 

10467 

1. Ribonuclease A (0.2mg/mL) 

2. Insulin (0.067mg/mL) 

3. Lysozyme (0.2mg/mL) 

4. Myoglubin (0.2mg/mL) 

5. Ovalbumin (0.4mg/mL) 

A. ProSphere HP C4 

B. Leading Competitive C4 

ProSphere HP 300Å HPLC Columns 












C18, 5µm Solvent Reducer 3.0 x 150mm 35519 














C4, 5µm Solvent Reducer 3.0 x 150mm 35548 




*5µm, 10µm particles and other dimensions are also available. 

Column: C4, 5μm, 300Å, 4.6 x 150mm (Part No. 35560) 

Mobile Phase: A: 0.01%TFA in Water 

B: 0.01%TFA in Acetonitrile 


%B: 20 100 



Injection Vol.: 10µL 

Even at very low concentrations of TFA, ProSphere HP C4 exhibits 

excellent recoveries; significantly better than a leading competitive 

C4 column. 

ProSphere HP 300Å All-Guard Cartridges* 


300Å C18, 5µm 3.0 x 7.5mm 3 35526 

4.6 x 7.5mm 3 35527 

HP C18, 3µm 


0.150 x 10mm — 22668 

0.300 x 10mm — 22669 

300Å C4, 5µm* 3.0 x 7.5mm 3 35565 

4.6 x 7.5mm 3 35566 

HP C4 Capillary Guard** 0.150 x 10mm — 22678 

0.300 x 10mm — 22679 


ea 80101 






**5µm, 10µm particles and other dimensions are also available. 

Table 1—Percent Protein Recovery at Low Sample Concentration 

Insulin Ribonuclease A Lysozyme Bovine Serum Albumin Human Serum Albumin 

Phase 

0.02mg/mL 0.4mg/mL 0.02mg/mL 0.4mg/mL 0.02mg/mL 0.4mg/mL 0.02mg/mL 0.4mg/mL 0.02mg/mL 0.4mg/mL 

ProSphere HP C18 68 94 74 94 45 87 65 90 66 87 

A Leading Competitive C18 13 90 0 84 0 74 0 63 0 63 

ProSphere HP C4 79 95 79 94 64 92 74 93 72 89 

A Leading Competitive C4 31 93 53 91 20 75 0 53 0 68 

Every column has good recoveries with high sample concentrations. How does your column compare at low sample concentrations? 

ProSphere HP 300Å phases outperform a leading competitor at 0.02mg/mL. 


Looking for ProSphere prep columns? 

See page 161. 

96 



Alltech ® ProSphere 

HP C18-AQ Columns 

Ideal for Peptide Mapping 

• Stable from 100% aqueous to 100% organic 

mobile phases 

• Compatible with a broad range of LC/MS compatible 

mobile phases 

ProSphere HP C18-AQ Specifications 

Base Material 

Silica 

Particle Size 3µm, 5µm 

Pore Size 

100Å 

pH Range 2–8 

Tryptic Digest of Cytochrome C 

0 10 20 30 40 50 Min. 

10492 

10493 

A. C18-AQ 

B. Competitive C18 

Alltech ® Macrosphere 

GPC Columns 

• 7µm GPC phases separate proteins, peptides, and polar 

synthetic compounds by size-exclusion 

Macrosphere GPC Exclusion Limits in Daltons 

Pore Size Included Excluded 

60Å 250 28,000 

100Å 2500 350,000 

150Å 4000 500,000 

300Å 7500 1,200,000 

Macrosphere GPC Columns 

Standard 

Part No. 


Part No. 


GPC 60Å Analytical 4.6 x 250mm 88129 88130 

Semi-Prep 7.5 x 300mm 88175 — 


Semi-Prep 7.5 x 300mm 88177 — 


Semi-Prep 7.5 x 300mm 88179 — 


Semi-Prep 7.5 x 300mm 88181 — 

Macrosphere GPC All-Guard Cartridges* 

Packing, 5µm i.d. x Length Qty. Part No. 

GPC 4.6 x 7.5mm 3 96133 


ea 80101 




Column: 5µm, 4.6 x 150mm (Part No. 35579) 

Mobile Phase: A: 5mM Acetic Acid in Water 

B: 5mM Acetic Acid in Acetonitrile 


%B: 0 40 




This water-wettable phase is ideally suited for LC/MS and delivers 

more peaks than traditional C18 phases. 

ProSphere HP C18-AQ HPLC Columns 


C18-AQ, 3µm Capillary 150µm x 50mm 35568 

Capillary 150µm x 150mm 35574 







C18-AQ, 5µm Solvent Reducer 3.0 x 150mm 35577 




ProSphere HP C18-AQ All-Guard Cartridges* 


C18-AQ, 5µm 3.0 x 7.5mm 3 35582 

4.6 x 7.5mm 3 35583 


ea 80101 



Alltech ® Macrosphere 

Ion-Exchange Columns 

• 300Å weak and strong ion-exchange phases separate 

proteins, enzymes, and other biologicals 

Macrosphere Ion-Exchange Specifications 

Exchange Group 

Exchange Capacity 

WAX Diethylaminoethane 0.13 meq/gram 

WCX Carboxylic Acid 0.49 meq/gram 

SAX Quaternary Ammonium 0.14 meq/gram 

SCX Sulfonic Acid 0.50 meq/gram 

Macrosphere Ion-Exchange Columns 


WAX, 7µm Analytical 4.6 x 150mm 71151 


WCX, 7µm Analytical 4.6 x 150mm 71157 


SAX, 7µm Analytical 4.6 x 150mm 72303 


SCX, 7µm Analytical 4.6 x 150mm 72308 


Macrosphere Ion-Exchange All-Guard Cartridges* 


WAX, 5µm 4.6 x 7.5mm 3 96135 

WCX, 5µm 4.6 x 7.5mm 3 96137 

SAX, 5µm 4.6 x 7.5mm 3 96139 

SCX, 5µm 4.6 x 7.5mm 3 96141 


ea 80101 




97


IC Columns Introduction 

hplc columns | ion chromatography 

Alltech ® IC Column Specifications 

Column Composition pH Range Applications 

Anion Exchange Columns 

Allsep polymer-based anion pH 2–10 inorganic anions, weak and strong acid ions, 

exchanger, 7µm 

metal complexes, organic acids 

Allsep A-2 polymer-based anion pH 2–11 inorganic anions, organic acids, suitable for 


both weak and strong anions in a single run 

Novosep A-2 polymer-based anion pH 3–12 inorganic anions and oxyhalides; ideal for 


separation of seven common anions plus 

three oxyhalide anions in one run 

AN1 

polymer-based anion 


Anion/S silica-based anion 


Anion/R polymer-based anion 


PRP-X100 polymer-based anion 


Cation Exchange Columns 

Universal Cation silica-based cation 


Universal Cation silica-based cation 

HR 


PRP-X200 polymer-based cation 


Organic Acid Columns 

PRP-X300 polymer-based sulfonated 

cation exchanger 

Anion Exclusion polymer-based cation 


Specialty Columns 

Transition Metal silica-based reversedphase, 

3µm and 7µm 

Surfactant/R 

Surfactant C8 

Surfactant C18 

polymer-based 

reversed-phase, 7µm 

silica-based reversedphase, 

5µm 

silica-based reversedphase, 

5µm 

EPA 

Methods 

Optimized for Suppressed 

or Non-Suppressed 

Conductivity 

300.0, 

Part A 

both 99 

300.0, both 99 

Part A 

300.0, suppressed 100 

300.1, 

317.0, 

326.0 

pH 2–13 inorganic anions, weak and strong acid ions, — both 100 

organic acids 

pH 2–5.5 inorganic anions — non-suppressed 101 

pH 2–12 inorganic anions, weak and strong acid ions — non-suppressed 101 

pH 1–13 inorganic anions, weak and strong acid ions — non-suppressed 103 

pH 2–7 

pH 2–7 

pH 1–13 

groups I and II cations, amines, divalent 

transition metals 

groups I and II cations, amines, divalent 

transition metals; smaller particle size for 

improved peak resolution 

groups I and II cations (separate runs), 

amines, lanthanides 

Page 

300.7 both 102 

300.7 both 102 

— non-suppressed 103 

pH 1–13 simple aliphatic carboxylic acids and alcohols — non-suppressed 103 

pH 1–13 organic acids, weak acid anions — non-suppressed 104 

pH 2–7 high capacity, separates divalent transition 

metals and metal-cyano complexes more 

selectively than a cation exchange column 

— non-suppressed 104 

pH 1–14 anionic and cationic surfactants; separate — both 105 

short and long chain surfactants in one 

gradient run 

pH 2–7 short chain anionic surfactants — non-suppressed 105 

pH 2–7 long chain or aromatic anionic surfactants — non-suppressed 105 

IC Guard Kits Cross Reference 

Kit includes holder and three guard cartridges. 

Guard Reference Table 

Guard Cartridge 

Type 

All-Guard Kit 

Part No. 

Brand Column Type 

Alltech Allsep, Allsep A-2 GA-1 38109 

Novosep A-2 GA-1 38109 

Anion/R, Anion/S GA-1 38109 

Universal Cation GC-2 27109 

Universal Cation HR GC-3 23115 

Anion Exclusion GIE-1 38117 

Transition Metal C18 GRP-2 38123 

Surfactant/R GRP-1 38122 

Surfactant C8, C18 GRP-2 38123 

Dionex 

IonPac ® AS4A-SC, AS7, AS11, GA-1 38109 

AS22, AS24, AS26, AS27 

IonPac ® CS3, CS10, CS11 GC-1 38113 

IonPac ® CS12, CS14, CS15 GC-2 27109 

IonPac ® NS1 GRP-1 38122 

IonPac ® AS1, AS5 GIE-1 38117 

Guard Reference Table (continued) 

Guard Cartridge 

Type 

All-Guard Kit 

Part No. 

Brand Column Type 

Waters IC-Pak-A GA-1 38109 

IC-Pak-M/D GC-2 27109 

Ion Exclusion GIE-1 38117 

Hamilton PRP-X100 GA-1 38109 

PRP-X200 GC-1 38113 

PRP-X300 GIE-1 38117 

Misc. Sarasep ANIONSEP AN1 GA-1 38109 

Bio-Rad ® , Aminex ® , HPX-87 GIE-1 38117 

Interaction ION-120 GA-1 38109 

Interaction ION-210 GC-1 38113 

Interaction ORH-801 GIE-1 38117 

Metrohm ® Metrosep GA-1 38109 

more info 

Cartridges and holders can also be purchased separately. 

See specific phase pages for replacement cartridge ordering 

information. 

98 



Alltech ® Anion Columns 

Allsep Anion IC Columns 

• Resolves fluoride away from the water dip 

• Meets requirements for U.S. EPA Method 300.0 part A 

The Allsep Anion Column is compatible with common IC 

mobile phases: carbonate, bicarbonate, p-hydroxybenzoic 

acid, phthalic acid, succinic acid, and sodium octane 

sulfonate. 

Use this column for performing EPA Method 300.0 Part A 

“Determination of Inorganic Anions in Water” per U.S. EPA 

guidelines. 

1 

2 

3 

4 

Resolves Fluoride Away 

from the Water Dip 

5 

6 

7 

13033 

1. Fluoride 

2. Chloride 

3. Nitrite 

4. Bromide 

5. Nitrate 

6. Phosphate 

7. Sulfate 

Allsep A-2 Anion IC Columns 

• Separates both weak and strong acid anions in one run 

• Resolves formate away from fluoride and chloride 

The Allsep A-2 Anion Column separates both weakly 

retained organic and strongly retained inorganic anions 

in one run. The high capacity Allsep A-2 Anion Column 

is also suitable for the separation of chlorite and chlorate. 

1 

3 

4 

2 

5 

6 

Organic and Inorganic Anions 

7 

8 

9 

10 

11 

8747 

1. Fluoride (1.0ppm) 

2. Acetate (1.0ppm) 

3. Formate (1.0ppm) 

4. Chlorite (1.0ppm) 

5. Chloride (2.0ppm) 

6. Nitrite (2.0ppm) 

7. Bromide (2.0ppm) 

8. Chlorate (5.0ppm) 

9. Nitrate (2.0ppm) 

10. Phosphate (3.0ppm) 

11. Sulfate (3.0ppm) 


0 2 4 6 8 10 12 14 16 18 20 22 24 Min. 

0 6 

12 18 24 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Allsep Anion, 7µm, 4.6 x 100mm 

(Part No. 51207) 

0.85mM NaHCO3/0.9mM Na2CO3 

1.2mL/min 

Suppressed Conductivity 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Allsep A-2 Anion, 7µm, 4.6 x 100mm 

(Part No. 51219) 

2.1mM NaHCO3/1.6mM Na2CO3 

2.0mL/min 


Allsep Anion Specifications 

Composition: 

Methacrylate based w/quaternary ammonium 

functional groups 

Particle Size: 7µm 

Mobile Phase Limits: pH 2–10, 0–100% organic modifier 

Allsep A-2 Anion Specifications 

Composition: 

Methacrylate based w/quaternary alkanol 

amine functional groups 



Allsep Anion Columns 

Packing i.d. x Length PEEK Part No. SS Part No. 

Allsep Anion 4.6 x 30mm 51217 51216 

4.6 x 50mm 51213 51214 

4.6 x 100mm 51207 51200 

4.6 x 150mm 51209 51208 

Allsep A-2 Anion Columns 


Allsep A-2 4.6 x 50mm 51221 51220 

4.6 x 100mm 51219 51218 


Save money with IC All-Guard Kits on page 98. Kit includes one 

holder and three cartridges. 

Guards for Allsep and AllsepA-2 Columns 


GA-1 Anion Cartridges 3 38108 

All-Guard Cartridge Holder* ea 80101 

*Direct-Connect column coupler included. 


99




Novosep A-2 Anion IC Columns 

• Separates seven inorganic anions and three oxyhalide 

anions in a single run 

• Meets the requirements of U.S. EPA Methods 300.0, 

300.1, 317.0, and 326.0 

The Novosep A-2 Anion Column is ideal for the 

separation of 10 inorganic anions by suppressor-based ion 

chromatography using U.S. EPA Method 300.1. The column 

can also be used to determine inorganic anions in drinking 

water per U.S. EPA Method 300.0 and trace bromate in 

drinking water using U.S. EPA Methods 317.0 and 326.0. 

1 

Anions in High Ionic Strength Water 

by EPA Method 300.0 

2 

3 

4 5 

67 

8 

9 10 11 

10121 

1. Fluoride 

2. Chlorite 

3. Bromate 

4. Chloride 

5. Nitrite-N 

6. Bromide 

7. Chlorate 

8. Dichloroacetate 

9. Nitrate-N 

10. Phosphate-P 

11. Sulfate 

IC Sep AN1 IC Column 

• Separates anions with carbonate/bicarbonate and 

hydroxide mobile phases 

The IC Sep AN1 Column is compatible with both 

suppressor-based and non-suppressed detection. A variety 

of mobile phases including sodium carbonate/bicarbonate, 

sodium hydroxide, and borate/gluconate may be used. 

Separation of Sulfite and Sulfate 

1 

2 3 4 5 

6 

7 

8 

6316 

1. Fluoride 

2. Chloride 

3. Nitrite 

4. Bromide 

5. Nitrate 

6. Phosphate 

7. Sulfite 

8. Sulfate 

0 2 4 6 8 10 12 14 16 18 20 22 24 26 Min. 

0 2 4 6 8 10 12 14 16 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Novosep A-2 Anion, 5µm, 4.0 x 250mm 

(Part No. 51410) 

3.6mM NaHCO3 

0.8mL/min 


Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

IC Sep AN1, 9µm, 4.6 x 250mm 

(Part No. 38140) 

1.8mM NaHCO3, 1.8mM Na2CO3 

1.0mL/min 


Novosep A-2 Anion Specifications 

Composition: 

Polyvinyl alcohol-based w/quaternary 

ammonium functional groups 


Mobile Phase Limits: pH 3–12, 0–100% organic modifier, 

10% modifier is practical upper limit 

IC Sep AN1 Specifications 

Composition: 

Poly(styrene-divinylbenzene) alkyl dimethyl 

ethanol ammonium functional group 


Exchange Capacity: 0.05meq/g 

Mobile Phase Limits: pH 2–13, No organic solvents 

Novosep A-2 Anion Column 

Packing i.d. x Length PEEK Part No. 

Novosep A-2 4.0 x 250mm 51410 

Guards for Novosep A-2 Anion Columns 









IC Sep AN1 Columns 


AN1 4.6 x 250mm 38140 

Choose a guard column or integral guard discs to protect your 

IC Sep Column. 

IC Sep Guards 


Guard Column, 50 x 4.6mm ea 38145 

Guard Column Guard-Disc 5 38146 

100 




Anion/S IC Columns 

• Silica-based for symmetrical peak shapes 

• Separates inorganic and organic anions 

The Anion/S Columns are best suited for routine separations 

of chloride, bromide, nitrate, and sulfate. Not suitable for 

fluoride analysis. 

1 

2 

3 4 

0 4 8 12 16 20 24 Min. 

EDTA and Other Anions 

8284 

1. Nitrite 

2. EDTA 

3. Nitrate 

4. Bromide 

Column: Anion/S, 10µm, 4.6 x 100mm (Part No. 269012) 

Mobile Phase: 1.5mM Sulfuric Acid 



Anion/R IC Columns 

• Polymer-based for broad pH stability 

• Separates seven common inorganic anions 

Because of their high pH stability, these columns are ideal 

for the separation of anions using high pH mobile phases 

such as p-hydroxybenzoate, sodium hydroxide, and sodium 

carbonate/bicarbonate. The larger dimension columns 

(150mm and 250mm) are suitable for a high-resolution 

separation of the seven common inorganic anions. 

1 

2 3 45 

6 

Weak Acid Anions 

5697 

1. Borate (5ppm) 

2. Silicate (10ppm) 

3. Formate (10ppm) 

4. Sulfide (10ppm) 

5. Chloride (20ppm) 

6. Cyanide (20ppm) 


Anion/S Specifications 

Composition: 

Silica w/quaternary ammonium ion 

exchanger 



Mobile Phase Limits: pH 2–5.5, 0–100% organic modifier 

0 9 18 Min. 

Column: Anion/R, 10µm, 4.1 x 250mm (Part No. 269029) 

Mobile Phase: 4mM Sodium Hydroxide/ 0.1mM Sodium Benzoate 


Detector: Conductivity 

Anion/S Columns 


Anion/S 4.6 x 100mm 269012 269013 

4.6 x 250mm 269011 269001 

Anion/R Specifications 

Composition: 

Poly(styrene-divinylbenzene) 

Trimethylammonium 


Exchange Capacity: 0.19 ± 0.02meq/g 





Anion/R Columns 


Anion/R 4.1 x 100mm — 269031 

4.1 x 250mm — 269029 

4.6 x 100mm 269036 — 

4.6 x 150mm 269034 — 

Guards for Anion/S and Anion/R Columns 






101



Alltech ® Cation Columns 

Universal Cation and Universal Cation HR IC Columns 

• Separate Groups I and II cations in one isocratic run 

• Separate transition metals without post-column reaction 

• New Universal Cation HR 3µm particle size for 

improved efficiency 

Compatible with a variety of mobile phases including 

complexing acids (citric acid, tartaric acid, oxalic acid), 

mineral acids (nitric acid, hydrochloric acid, sulfuric acid) 

and non-complexing organic acids (methanesulfonic acid). 

12 

3 

4 

1. Lithium (0.2ppm) 

2. Sodium (1.5ppm) 

3. Ammonium (1.5ppm) 

4. Potassium (2.5ppm) 

5 6 7 

8 

Metal Ions 

0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 Min. 

9 

10318 

5. Nickel (2.5ppm) 

6. Zinc (2.5ppm) 

7. Cobalt (2.5ppm) 

8. Magnesium (2.0ppm) 

9. Calcium (2.0ppm) 

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min. 

Column: 

12 3 

4 

Mobile Phase: 

Flow Rate: 

Detector: 

5 

Cesium and Strontium 

6 

Universal Cation, 7µm, 4.6 x 100mm 

(Part No. 27106) 

3mM Methanesulfonic Acid 

1.0mL/min 

Conductivity 

7 

8 

13035 

1. Lithium (0.12ppm) 

2. Sodium (0.90ppm) 

3. Ammonium (0.90ppm) 

4. Potassium (1.5ppm) 

5. Cesium (0.8ppm) 

6. Magnesium (1.2ppm) 

7. Calcium (1.2ppm) 

8. Strontium (1.6ppm) 

Column: Universal Cation, 7µm, 4.6 x 100mm 

(Part No. 27106) 

Mobile Phase: 2mM Tartaric Acid/1mM Oxalic Acid 



Universal Cation Specifications 

Composition: 

Silica coated with polybutadiene/maleic acid 

copolymer 


3µm and 7µm, spherical 


2 

3 

4 

1 

Rapid Analysis of Monovalent 

and Divalent Cations 

5 

6 

13013 

1. Lithium 

2. Sodium 

3. Ammonium 

4. Potassium 

5. Magnesium 

6. Calcium 

Universal Cation Columns 


7µm 4.6 x 100mm 27106 27100 

3µm HR 4.6 x 100mm — 23100 

7.0 x 53mm — 23109 





0 1 2 3 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

4 5 6 7 8 9 Min. 

Universal Cation HR, 3µm, 7.0 x 53mm 

(Part No. 23109) 


2.5mL/min 

Conductivity 





Guards for Universal Cation Columns 


GC-2 Universal Cation 3 271010 

GC-3 Universal Cation HR 3 23110 






102 



Hamilton ® Anion, Cation, and Organic Acid Columns 

PRP-X100 Anion Columns 

High pH stability makes this column ideal for the separation 

of weak anions. PRP TM -X100 provides excellent resolution 

for the seven most frequently analyzed inorganic anions. 

For non-suppressed analyses only. 

PRP-X200 Cation Analysis Columns 

For separation of the alkali metal and alkaline earth cations 

in separate runs. 

PRP-X300 Organic Acid Columns 

For separation of simple aliphatic carboxylic acids and 

alcohols. Inorganic anions are not retained. 

1 

EDTA in Eye Drops 

8068 

1. CuEDTA 

Hamilton ® PRP-X100 Specifications 

Composition: 

Trimethylammonium psDVB copolymer 


10µm, spherical 




Composition: 

Sulfonated psDVB copolymer 


10µm, spherical 




Composition: 

Sulfonated psDVB in H+ form 

Particle Size: 3µm and 7µm 


1 

2 

3 

Alkali Metals 

5204 

1. Lithium 

2. Sodium 

3. Ammonium 

4. Potassium 

5. Cesium 


4 5 

0 2 4 6 8 10 12 Min. 

Column: PRP-X100, 10µm, 4.1 x 150mm (Part No. 79434) 

Mobile Phase: 0.003N Sulfuric Acid 



0 1 2 3 4 5 Min. 

Column: PRP-X200, 10µm, 4.1 x 150mm (Part No. 79441) 

Mobile Phase: 0.004N HNO3 in 30% MeOH 



Hamilton ® PRP-X100 Columns 


PRP-X100 2.1 x 150mm 79348 

2.1 x 250mm 79346 

4.1 x 100mm 79439 

4.1 x 150mm 79434 

4.1 x 250mm 79433 


Particle Size i.d. x Length Part No. 

PRP-X200 2.1 x 150mm 79394 

2.1 x 250mm 79347 

4.1 x 150mm 79441 

4.1 x 250mm 79442 


Particle Size i.d. x Length Part No. 

PRP-X300, 3µm 4.1 x 150mm 79819 

PRP-X300, 7µm 4.1 x 150mm 79464 

4.1 x 250mm 79465 

4.6 x 150mm 79485 

Hamilton ® PRP-X100 Guard Kits and Cartridges 


Guard Column Starter Kit 

ea 79448 

PRP-X100, 1 Holder, 2 Cartridges 

Replacement Guard Cartridges 

(Requires Hamilton ® Guard Holder) 

PRP-X100, 25 x 2.3mm 

5 79446 




ea 79456 




PRP-X200, 25 x 2.3mm 

5 79449 




ea 79460 




PRP-X300, 25 x 2.3mm 

5 79453 


103



Alltech ® Anion 

Exclusion Columns 

• Separate organic acids and weak acid anions 

• Polymer-based for broad pH stability 

• Economical choice for small inorganics 

Anion Exclusion Columns separate organic acids and weakly 

ionized anions by an anion exclusion mechanism. Dilute 

mineral acids are the typical mobile phases for the separation. 

Acetonitrile may be used as an organic modifier to decrease 

the retention of hydrophobic compounds. 

1 

2 

Organic Acid Standards 

3 

13037 



3. Malic Acid 




Alltech ® Transition 

Metal Columns 

• Separate divalent transition metals and metal-cyano 

complexes more selectively than a cation exchange 

column 

To separate divalent transition metals, use conductivity 

detection for concentrations as low as 1ppm, or UV detection 

with post-column reaction for ppb-level concentrations 

To separate metal-cyano complexes, use the Alltech ® low 

UV IPC A (Part No. 185149) as the ion-pair reagent, and 

methanol or acetonitrile as the organic modifier. 

Metal-Cyano Complexes 

5358 

4 5 

6 8 10 12 14 16 18 20 22 24 Min. 

6 

1 

2 3 4 

5 

1. Fe(CN)6 4- 

2. Ag(CN)2 - 

3. Fe(CN)6 3- 

4. Ni(CN)4 2- 

5. Au(CN)2 - 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Anion Exclusion, 10µm, 7.5 x 300mm 

(Part No. 269062) 

1.5mM Sulfuric Acid 

0.4mL/min 

Conductivity 

0 10 20 30 40 Min. 

Anion Exclusion Specifications 

Composition: 

Highly sulfonated poly (styrene-divinylbenzene) 

cation exchanger 


Mobile Phase Limits:


Alltech ® Surfactant Columns 

Surfactant/R IC Columns 

• Separate anionic and cationic surfactants 

• Separate short and long chain surfactants in one 

gradient run 

The Surfactant/R Column separates anionic and cationic 

surfactants by ion-pair chromatography along with suppressed 

conductivity detection. This column can also be used for other 

reversed-phase applications. 

1 

2 

3 

Short and Long Chain 

Cationic Surfactants 

4 

5 

6 

7 

10317 

1. Tetramethyl Ammonium (20ppm) 

2. Tetraethyl Ammonium (20ppm) 

3. Tetrapropyl Ammonium (20ppm) 

4. Tetrabutyl Ammonium (30ppm) 

5. Dodecyltrimethyl Ammonium (30ppm) 

6. Cetyltrimethyl Ammonium (40ppm) 

7. Myristyltrimethyl Ammonium (50ppm) 

Surfactant/C IC Columns 

• For non-suppressed analyses 

• Silica-based for symmetrical peak shapes 

Choose Surfactant C8 for short chain anionic surfactants 

such as alkyl sulfates and alkyl sulfonates. 

Choose Surfactant C18 for long chain or aromatic 

surfactants such as xylene sulfonate and dodecyl benzene 

sulfonate. 

1 

Alkyl Sulfates 

5998 

1. Octyl Sulfate 

2. Decyl Sulfate 

3. Dodecyl Sulfate 


0 2 4 6 8 10 12 14 16 18 20 22 Min. 

Column: 

Surfactant/R Column, 7µm, 4.6 x 150mm 

(Part No. 25101) 

Mobile Phase: A: 2mM Nonafluoropentanoic Acid 

B: 100% Acetonitrile 


%B: 30 30 35 80 


Detector: 


2 

0 6 12 18 24 30 Min. 

3 

Surfactant/R Specifications 

Composition: 

Polydivinylbenzene (DVB) based resin 



Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Surfactant C8, 5µm, 4.6 x 250mm 

(Part No. 269072) 

0.02g/L Ammonium Acetate in 40% Methanol 

1.2mL/min 

Conductivity 

Surfactant/R Columns 


Surfactant/R, 7µm 4.6 x 150mm 25101 25100 

Guards for Surfactant/R Columns 


GRP-1 Revised Phase 3 38124 










Surfactant/C Specifications 

Composition: 

Silica-based C8 or C18 



Surfactant/C Columns 


C8, 5µm 4.6 x 250mm 269072 269092 

C18, 5µm 4.6 x 250mm 269071 269091 

Guards for Surfactant/C Columns 


GRP-2 Revised Phase 3 38129 




105


Custom HPLC Columns 

Ordering Instructions 

Alltech offers a large selection of packing materials and formats to create 

your own custom HPLC column. 

hplc columns | custom 

Step 1: Select your packing material and particle size from Table 1. 

Step 2: Select your column format from Table 2. 

Step 3: Call your local Customer Service office for a quote. Indicate phase, 

particle size, format, and dimensions. 

Table 1—HPLC Packing Materials 

Family Phase Particle Sizes 

Adsorbosphere 

C18, C8, CN, NH2, Silica, HS C18, HS Silica, UHS C18, Phenyl, CN-AQ, SAX, SCX, OPA HR, OPA 3, 5, 10µm 

HS, CN-AQ, SAX, SCX, OPA HR, OPA HS 

Adsorbosphere XL C18 300, C8 300, C18, C8, Silica, SAX, SCX, TMS 3, 5, 10µm 

Allsphere C8, CN, NH2, ODS-2, Silica, C1, ODS-1, Phenyl, C6, SAX, SCX 3, 5µm 

Alltima C18, C8, Silica, CN, NH2, Phenyl, C18-LL, C18-WP, C4-WP 3, 5, 10µm 

Alltima HP C18, C18 Amide, C18 EPS, C18 HiLoad, C8, CN, Silica 1.5, 3, 5µm 

Apollo C18, C8, Phenyl, Silica 5µm 

Brava C18 BDS, C18 ODS, C8, Phenyl, Cyano BDS, Cyano, Amino, Silica 3, 5µm 

Carbohydrate Anomer, NH2 10µm 

Econosphere C18, C8, Silica, CN, NH2 3, 5, 10µm 

Everest ® C18 5, 10, 15–20µm 

GraceAlpha C18, C8, Silica 5, 10, 15, 20µm 

Grom-Sil ODS-0, ODS-1, ODS-3, ODS-4, ODS-5, ODS-6, ODS-7, C8, C6, C4, C1, CN, NH2, DIOL, IEX, Silica 1.5, 3, 5, 7, 10µm 

Grom-Sapphire C18, C8, C4, Silica 3, 5, 10µm 

Ion Chromatography Transition Metal, Universal Cation, Surfactant C8, Surfactant C18, Surfactant/R, Allsep, Allsep 5, 7, 10µm 

A-2, Novosep A-1 

Kromasil ® , Akzo-Nobel C18, C4, C8, NH2, Silica 5, 10µm 

LiChrosorb ® , Merck NH2, RP-18, RP-8, RP-Select B, Silica-60, Silica-100 5, 10µm 

LiChrospher ® , Merck RP-18, RP-18 Endcapped, RP-8, RP-8 Endcapped, Silica-60, CN, Diol, NH2, Silica-100, RP-Select B 3, 5µm 

Macrosphere GPC 60, 100, 150, 300, SAX 300, SCX 300, WAX 300, WCX 300 5, 7µm 

Mixed-Mode C18/Cation, C8/Anion, C8/Cation 5, 7µm 

Nucleosil ® , Macherey-Nagel C18, C8, CN, NH2, SA, SB, Silica, Phenyl 3, 5, 10µm 

Partisil, Whatman C8, ODS-2, ODS-3, PAC, SAX, Silica, ODS, SCX 5, 10µm 

Platinum 

C18-300, EPS C18-300, Silica-300, C18, EPS C18, C8, C18, EPS C18, C8, EPS C8, CN, NH2, 1.5, 3, 5µm 

Phenyl, Silica, SAX 

Prevail Amide, Amino, C18, C18 Select, C8, CN, Phenyl, Silica 3, 5µm 

ProSphere HP C18 300, C18 100, C4 300 1.5, 3, 5, 10µm 

Spherisorb ® , Waters C8, CN, NH2, ODS-2, Silica, ODS-1, Phenyl, C6, SAX, SCX 3, 5µm 

Vydac ® MS C18, C8, C4, Diphenyl 3, 5, 10–15µm 

Vydac ® TP C18, C8, C4, Diphenyl 3, 5, 10–15µm 

7102a 

Table 2—Custom Part Numbers for HPLC Column Formats 

Format Custom Part No. Description Available Lengths Available i.d.s 

Capillary Call Quartz glass Grace capillary columns connect directly to 5, 10, 20, 50, 75, 100, 0.075, 0.1, 0.2, 0.3, 0.5, 0.8mm 

microinjector and via fused silical capillary detectors 150, 250mm 

Analytical, Solvent- C-6000 Stainless steel analytical column with industry standard 30, 50, 100, 150, 250, 2.1, 3.0, 3.9, 4.1, 4.6mm 

Reducer, and LC/MS 

female fittings. 

300mm 

Analytical, Waters ® C-6000 Stainless steel analytical column with Waters ® -style 30, 50, 100, 150, 250, 2.1, 3.0, 3.9, 4.1, 4.6mm 

Style Endfittings 

endfittings. 

300mm 

Metal-Free* C-6000 Metal-Free columns with industry standard female 30, 50, 100, 150, 250, 2.1 (30 or 100mm lengths only), 

fittings.* 

300mm 

4.6, 7.5mm 

Rocket C-6000 Fast-analysis columns with Rocket endfittings; 33, 53mm 7mm 

requires 3µm or smaller particle size. 

All-Guard Cartridges C-9800 4/pk All-Guard Cartridges, Stainless Steel. Purchase 7.5mm 

2.1, 3.0, 4.6mm 


holder 80101 separately. 

All-Guard Cartridges C-9400 4/pk All-Guard Cartridges, PEEK. Purchase holder 7.5mm 

4.6mm 

Metal-Free* 

80101 separately. 

Preparative** C-6000F Stainless steel preparative column with threaded 70, 100, 250, 500mm 7, 10, 22mm 

endfittings. 

Capillary Guards Call Create a zero dead volume finger-tight connection to 5, 10, 20mm 0.1, 0.15, 0.3, 0.5mm 

capillary columns. 

*Metal-Free columns are available with ProSphere and Ion Chromatography packings only. 

**Additional custom prep columns available, see pages 162–170. 

more info 

For more information on our column formats, see page 31. 

106 



Empty HPLC Hardware 

Stainless Steel Analytical Column Hardware 

• Threaded tube for easy assembly 

• Type 316 stainless steel construction 

• Column i.d. of 2.1, 3.2, 3.9, 4.1, and 4.6mm 

Endfitting 

Ferrule 

Threaded Column Tube 

Frit/Insert 

Endfitting 

Stainless steel analytical columns come complete with the 

threaded tube, two 2µm industry standard frit/inserts, and two 

stainless steel endfitting nuts. Removable color-coded bands 

available separately, (Part No. 93237). Industry standard 

frit/inserts contain a stop depth equal to 0.100" (see Figure 1). 

Optional 0.5µm porosity frit/inserts are also available. 

5319 

Compression 

Screw 

Stop Depth 

(0.100") 


Analytical Column 

Frit/Insert 

Figure 1—Stainless steel analytical columns have ports with industry 

standard stop depth. 

Packing Adapter 

0765 

hplc columns | hardware 

Adapters and sealing rings are available for slurry packing 

your own columns (see Figure 2). The packing adapter 

comes with a 1/4" precolumn tube to connect to 1/4" slurry 

reservoirs. 

Stainless Steel Analytical Column Hardware Specifications 

Material: 


Max. Temperature: 100ºC 

Max. Pressure 

10,000psig 

Thread Type: 

10-32 UNF (CPI Standard Ports) 

Typical Use: 

Analytical HPLC Columns 

Sealing Ring 

Figure 2—Packing adapter threads directly onto stainless steel 

analytical column tube. 

2730 

Stainless Steel Analytical Hardware and Accessories 

2.1mm i.d. 

3.2mm i.d. 

3.9mm i.d. 

4.1mm i.d. 

4.6mm i.d. 

Description 

Part No. 

Part No. 

Part No. 

Part No. 

Part No. 

Complete Columns 

50mm 65175 65170 65165 65160 65150 

100mm 65176 65171 65166 65161 65152 

150mm 65177 65172 65167 65162 65154 

250mm 65178 65173 65168 65163 65156 

300mm 65179 65174 65169 65164 65158 


Endfitting (ea) 75001 75001 75001 75001 75001 

Frit/Insert, 2µm (5/pk) 75028 75026 75024 75022 75020 

Frit/Insert, 0.5µm (5/pk) 75029 75027 75025 75023 75021 

Packing Accessories 

Packing Adapter (ea) 65189 65188 65187 65186 65185 

Sealing Rings (10/pk) 65199 65198 65197 65196 65195 


107



Empty HPLC Hardware 

Stainless Steel Preparative Column Hardware 

• Threaded format for easy assembly 

• Extended column life with the patented continuously 

adjustable piston (CAP) 

• Column i.d. of 7, 10, and 22mm 

The Alltech ® easy-to-use preparative format has threaded 

endfittings and a continuously adjustable piston/frit (CAP). 

The piston/frit extends column life by compressing the packed 

bed to remove voids. 

Column Tube 

Continuously 

Adjustable 

Piston/Frit 

Endfitting 

Stainless Steel Swaged Column Hardware 

• Passivated stainless steel for a more inert surface 

• Uses precision bore tubing and OptiFlow endfittings 

• Column i.d. of 2.1, 3.2, 4.6, 10, and 22.5mm 

Stainless steel swaged hardware uses precision bore 

seamless tubing and OptiFlow endfittings. It is the ideal 

choice when standard column lengths are not desirable— 

just cut to your specification. OptiFlow endfittings have a 

flow distributor which spreads the sample and eluant across 

the entire frit surface to improve peak symmetry and prevent 

frit blockage. 

2µm 

SS Frit 

Distributor 

Disk 

Endfitting 

Tubing 

Nut and 

Ferrule 

5544 

5568 

Stainless Steel Preparative Column Hardware Specifications 

Material: 

316 Stainless Steel, PEEK 


Max. Pressure 

8000psig 

Thread Type: 

10-32 UNF (CPI Standard Ports) 

Typical Use: 

Prep HPLC Columns 

Precision Bore HPLC Column Tubing Specifications 

Material: 



Max. Pressure 

8000psig 

Typical Use: 

HPLC Column Assembly w/Swageable 

Endfittings 

Stainless Steel Preparative Hardware and Accessories 

i.d. Length Qty. Part No. 

Complete Columns 

7mm 100mm ea 96500 

7mm 250mm ea 96501 

7mm 500mm ea 96502 

10mm 100mm ea 96510 

10mm 250mm ea 96511 

10mm 500mm ea 96512 

22mm 70mm ea 96524 

22mm 100mm ea 96520 

22mm 250mm ea 96521 

22mm 500mm ea 96522 

Replacement Endfittings 

7mm — ea 96505 

10mm — ea 96515 

22mm — ea 96525 

Replacement Piston/Frit, 2µm 

7mm — ea 96507 

10mm — ea 96517 

22mm — ea 96527 

Packing Adapters* 

7mm — ea 96508 

10mm — ea 96518 

Packing Seals* 

7mm — 10 96509 

10mm — 10 96519 

*22mm packing adapters and seals are not available. 

Precision Bore Tubing 

Per Foot 

Part No. 

Per Meter 

Part No. 

o.d. x i.d. 

Precision Bore Tubing 

1/8" x 2.1mm 3012 30120 

1/4" x 2.1mm 3013 30130 

1/4" x 3.2mm 3011 30110 

1/4" x 4.6mm 3015 30150 

OptiFlow Endfittings* 

Description For use with Tubing i.d. Qty. Part No. 

OptiFlow Endfittings 

1/4" 2.1, 3.2, 4.6mm ea 400612 

3/8" 7.0mm ea 10123 

1/2" 10.0mm ea 9205 

1" 2.25mm ea 9204 

*Each fitting is supplied with a nut and ferrule for 1/16" tubing, a 2-micron stainless steel 

frit and distributor disk. 


Additional prep hardware also available. See page 150. 

108 www.discoverysciences.com


Stainless Steel Frits 

• Type 316 stainless steel 

• Heat-passivated for a more inert surface 

Stainless Steel Frit Specifications 

Material: 



Max. Pressure: 10,000psig 

Typical Use: 

Column Inlet/Outlet 

Stainless Steel Frits 

5/pk 

Part No. 

25/pk 

Part No. 

Frit Diameter Thickness Porosity 

1/4" (6.350mm) 0.031" (0.79mm) 0.5µm 720805 720825 

0.031" (0.79mm) 2.0µm 721005 721025 

0.063" (1.59mm) 2.0µm 716505 716525 

3/8" (9.525mm) 0.031" (0.79mm) 0.5µm 721205 721225 

0.031" (0.79mm) 2.0µm 721405 721425 

0.063" (1.59mm) 0.5µm 718005 718025 

0.063" (1.59mm) 2.0µm 718205 718225 

1/2" (12.7mm) 0.031" (0.79mm) 0.5µm 721605 721625 

0.031" (0.79mm) 2.0µm 721805 721825 

1" (25.4mm) 0.031" (0.79mm) 2.0µm 722205 722225 

0.063" (1.59mm) 2.0µm 718605 718625 

best 

value 


Encased Stainless Steel Frits 

• Improved sealing over conventional frits 

• Better mechanical stability 

Encased Stainless Steel Frit Specifications 

Material: 

316 Stainless Steel, PEEK, or Graph-Tite 



Typical Use: 


Encased Stainless Steel Frits 

0.5 Micron 

(5/pk) 

Part No. 

2 Micron 

(5/pk) 

Part No. 

2 Micron 

(10/pk) 

Part No. 

2 Micron 

(20/pk) 

Part No. 

Frit Diameter Ring Diameter Thickness 

Stainless Steel Frits with PEEK Ring 

0.083" (2.1mm) 0.250" (6.4mm) 0.063" (1.59mm) — — 9900 99001 

0.125" (3.2mm) 0.250" (6.4mm) 0.063" (1.59mm) — — 9902 99021 

0.156" (4.0mm) 0.250" (6.4mm) 0.063" (1.59mm) — — 9906 99061 

0.273" (7.0mm) 0.375" (9.5mm) 0.063" (1.59mm) — — 9910 99101 

0.352" (10mm) 0.500" (12.7mm) 0.063" (1.59mm) — — 9912 99121 

Stainless Steel Frits with Carbon-Reinforced PEEK Ring 

0.083" (2.1mm) 0.250" (6.4mm) 0.031" (0.79mm) 75015 75014 — — 

0.125" (3.2mm) 0.250" (6.4mm) 0.031" (0.79mm) 75013 75012 — — 

0.181" (4.6mm) 0.250" (6.4mm) 0.031" (0.79mm) 75006 75005 — — 

Encased Metal-Free PEEK Frits 

• Metal-free inert construction 

• Ideal for chromatography biocompatibility applications 

Encased Metal-Free PEEK Frit Specifications 

Material: 

PEEK 



Typical Use: 


Encased Metal-Free PEEK Frits 

5 Micron 

(5/pk) 

Part No. 

10 Micron 

(5/pk) 

Part No. 

Frit Diameter Ring Diameter Thickness 

PEEK Frits with PEEK Ring 

0.125" (3.2mm) 0.250" (6.4mm) 0.063" (1.59mm) 69025 69026 

0.181" (4.6mm) 0.250" (6.4mm) 0.063" (1.59mm) 68152 69027 

0.312" (7.9mm) 0.375" (9.5mm) 0.063" (1.59mm) 67152 69028 


109



Empty Low-Pressure Hardware 

Omnifit ® Low-Pressure Column Hardware 

• Borosilicate heavy wall glass with precision threaded 

ends and patented “strain free” endfittings 

• Bore diameter constant to ±0.1mm 

• Higher pressure limits than other low-pressure column 

hardware 

Each Omnifit ® glass column includes two PTFE end pieces 

(one fixed, one adjustable), two 1/4-28 polypropylene outer 

caps, one frit kit (two each: 10µm polyethylene, 10µm PTFE 

and 25µm PTFE), two flangeless tube endings, two gripper 

fittings, and two meters of 1/16" PTFE tubing. 

Polypropylene 

Outer Cap 

Adjustable 

End Piece 

O-ring 

Frit 

Glass Column 

Polypropylene 

O-ring Outer Cap 

Frit 

Fixed End 

Piece 

Omnifit ® Column Hardware Specifications 

Material: 

Borosilicate Glass, PTFE, Fluoroelastomer 

Polymer, Polypropylene, and Polyethylene 


Max. Pressure: Varies, See Price Block 

Thread Type: 

1/4-28 UNF 

Typical Use: 

Low-pressure, Metal-free Columns 

2719 

5551 

Omnifit ® Glass Columns 

Length 

Part No. 

6.6mm i.d. Column, rated to 60Atm (900psig) 

100mm 62271 

150mm 62272 

250mm 62273 

400mm 62276 

10mm i.d. Column, rated to 40Atm (600psig) 

100mm 61110 

150mm 61120 

250mm 61140 

500mm 61160 

1000mm 61180 


100mm 64110 

150mm 64120 

250mm 64140 

500mm 64160 

1000mm 64180 


150mm 62121 

250mm 62141 

500mm 62161 

1000mm 62181 


Looking for low-pressure accessories? 

Low-pressure fittings, pages 117–120; 

Low-pressure tubing, page 387. 

Omnifit ® Glass Column Accessories 


6.6mm i.d. Column Accessories 

Adjustable Plunger ea 62287 

25µm Polyethylene Frits 20 62281 

25µm PTFE Frits 20 62282 



Fluoroelastomer Polymer O-rings 10 62285 

Silicone O-rings 10 62286 

10mm i.d. Column Accessories 


























Prefilters 

Direct-Connect Filter Kit 

• Threads into column inlet for 

minimal dead volume 

• Interchangeable tips connect 

to all column formats 

• Inexpensive replaceable filter 

elements 

SofGrip 

Fitting 


Filter House 

Tips 

6382 

In-Line Filter Kit 

• Available in metal-free or 

316 stainless steel 

• Comes with connecting fittings 

• Replaceable filter element 

Metal-Free 

In-Line Filter 

5557 


In-Line Filter 

6610 

Universal Mini Filters 

• Low volume direct-connect design 

• Ideal for LC/MS applications 

• Depth filter for greater capacity without band spreading 

Rheodyne ® Column In-Line Filter Kit 

• Install between injector and column 

• Use 3mm filter kit for 4.6mm columns 

• Use 1.5mm filter kit for 1 and 2mm 

columns 

6679 

5420 

High-Pressure In-Line Column Prefilter 

• Install between injector and column 

• Filter has surface area of 7.1mm 2 

High-Capacity In-Line Filters 

• Place between the pump 

and the injector 

• Filter has a surface area of 2.5cm 2 

Prefilters 

Description Material Max. Temp. Max. Pressure Thread Type Porosity Qty. Part No. 

Direct-Connect TM Filter Kit and Replacement Parts 

Direct Connect Filter Kit 316 SS, PTFE, and Kel-F ® 100°C 5000psig 10-32 UNF 2.0µm ea 28689 

Housing 316 Stainless Steel 100°C 5000psig 10-32 UNF — ea 27005 

Filter Stainless Steel 100°C 5000psig 10-32 UNF 2.0µm 5/pk 28640 

Female Tip Kel-F ® 100°C 5000psig 10-32 UNF — ea 28002 

Waters ® Tip Kel-F ® 100°C 5000psig 10-32 UNF — ea 28004 

Valco ® Tip Kel-F ® 100°C 5000psig 10-32 UNF — ea 28006 

Distributor Disks PTFE 100°C 5000psig 10-32 UNF — ea 28008 

In-Line Filter Kit and Replacement Parts 

Metal-Free Filter Kit* PEEK, PAT ® , and Graph-Tite 100°C 5000psig 10-32 UNF 2.0µm ea 68250 

Stainless Steel Filter Kit** 316 SS and Graph-Tite 100°C 5000psig 10-32 UNF 2.0µm ea 27000 

Filter PAT ® 100°C 5000psig 10-32 UNF 2.0µm 5/pk 68152 

Filter Stainless Steel 100°C 5000psig 10-32 UNF 2.0µm 5/pk 28640 

Housing 316 Stainless Steel 100°C 5000psig 10-32 UNF — ea 27010 

Universal Mini Filters † 

Mini Filter 1.2µL internal volume 316 Stainless Steel 100°C 7000psig 10-32 UNF 0.5µm 5/pk 77166 



High-Pressure In-Line Column Prefilter and Replacement Parts 

1/16" o.d. SSI Prefilter 316 Stainless Steel 500°C 15,000psig 1/4-28 UNF 2.0µm ea 05-0148 

1/16" o.d. SSI Prefilter 316 Stainless Steel 500°C 15,000psig 1/4-28 UNF 0.5µm ea 05-0149 

1/16" o.d. Industry Std. CPI Prefilter 316 Stainless Steel 500°C 15,000psig 10-32 UNF 0.5µm ea 25-0149 

1/16" o.d. Waters ® Prefilter 316 Stainless Steel 500°C 15,000psig 10-32 UNF 2.0µm ea 35-0148 

1/16" o.d. Waters ® Prefilter 316 Stainless Steel 500°C 15,000psig 10-32 UNF 0.5µm ea 35-0149 

Filter Elements 316 Stainless Steel 500°C 15,000psig — 2.0µm 10/pk 05-0154 

Filter Elements 316 Stainless Steel 500°C 15,000psig — 0.5µm 10/pk 05-0155 

Rheodyne ® Column In-Line Filter Kits †† and Replacement Parts 

1.5mm Filter Diameter Kit 316 Stainless Steel 500°C 7000psig 10-32 UNF 0.5µm ea 7315RV 

3mm Filter Diameter Kit 316 Stainless Steel 500°C 7000psig 10-32 UNF 0.5µm ea 7335RV 

1.5mm Filter Element 316 Stainless Steel 500°C 7000psig 10-32 UNF 0.5µm ea 7315-010 

3mm Filter Element 316 Stainless Steel 500°C 7000psig 10-32 UNF 0.5µm ea 7335-010 

High-Capacity In-Line Filters and Replacement Parts 

SSI Filter 316 Stainless Steel 500°C 15,000psig 1/4-28 UNF 0.5µm ea 050105 

SSI Filter 316 Stainless Steel 500°C 15,000psig 1/4-28 UNF 2.0µm ea 050108 

Industry Standard CPI 316 Stainless Steel 500°C 15,000psig 10-32 UNF 0.5µm ea 250105 

Industry Standard CPI 316 Stainless Steel 500°C 15,000psig 10-32 UNF 2.0µm ea 250108 

Filter Elements 316 Stainless Steel 500°C 15,000psig — 0.5µm 2/pk 050106 

Filter Elements 316 Stainless Steel 500°C 15,000psig — 2.0µm 2/pk 050107 

*Includes PEEK holder, PAT ® filter, and two SofGrip fittings. **Includes 316 Stainless Steel holder, Stainless Steel filter, and two SofGrip fittings. 

†Patent #5, 525, 503; #5,730,943; #5,911,954. ††Includes one holder, one filter, three fittings, and two connecting tubes 6cm x 0.007" i.d. 

5561 

5560 

hplc accessories | prefilters 


111


hplc accessories | fittings 

SofGrip One-Piece 

High-Pressure Fittings 

• Soft elastomer cushion makes hand-tightening 

comfortable 

• Graph-Tite construction is three times stronger 

than PEEK 

• One-piece integrated ferrule eliminates handling 

small pieces 

• No-Slip version stays on tubing between connections 

SofGrip One-Piece Fitting Specifications 

Material: 

Graph-Tite (Carbon-Reinforced PEEK) 

Max. Temperature: 100°C 

Max. Pressure: 5000psig 

Thread Type: 

10-32 UNF 

Typical Use: 

1/16" o.d. Tubing Connections 

One-Piece High-Pressure Fittings 

• PEEK or Graph-Tite construction is inert and biocompatible 

• One-piece integrated ferrule eliminates handling small pieces 

• No-Slip version stays on tubing between connections 

best 

seller 

Other 

Fittings 

No-Slip 

SofGrip 

Integral barrel tines in No-Slip SofGrip fittings lightly grip 

tubing. This holds the fitting in place between connections. 

4615 

4614 

6361 

One-Piece Fitting Specifications 

Material: 

PEEK or Graph-Tite (Carbon-Reinforced PEEK) 


Max. Pressure: Finger-Tight: 5000psig / Wrench-Tight: 8000psig 

Thread Type: 10-32 UNF 

Typical Use: 1/16" o.d. Tubing Connections 

5425 

A 

B 

C 

D 

E 

F 

One-Piece High-Pressure Fittings 

Graphic Length Color Material Qty. Standard Part No. No-Slip Part No. 

SofGrip One-Piece Fittings 

A Short Black Graph-Tite 10 40501 40511 

A Short Red Graph-Tite 10 40502 40512 

A Short Blue Graph-Tite 10 40503 40513 

A Short Green Graph-Tite 10 40504 40514 

A Short Yellow Graph-Tite 10 40505 40515 

B Long Black Graph-Tite 10 40506 40516 

A Short Assort. Graph-Tite 10 40507 40517 

One-Piece Fittings, Finger-Tight 

C Short Natural PEEK 10 32233 32293 

C Short Red PEEK 10 32072 32936 

C Short Yellow PEEK 10 32074 32935 

C Short Blue PEEK 10 32076 32933 

C Short Green PEEK 10 32078 32931 

C Short Black Graph-Tite 10 32343 31001 

C Short Assort. PEEK 12 32070 32937 

D Long Natural PEEK 10 32235 — 

D Long Black Graph-Tite 10 — 31002 

One-Piece Fittings, Hex-Head 

E Short Natural PEEK 10 32236 32297 

E Short Black Graph-Tite 10 32347 31003 

F Long Natural PEEK 10 32238 32299 

F Long Black Graph-Tite 10 32349 31004 



Two-Piece High-Pressure Fittings 

Double-Sided Ferrule (DSF) Fittings 

• Double-sided ferrule (DSF) provides twice the 

gripping power 

• PEEK construction is inert and biocompatible 

• Economical two-piece design 

DSF fittings have a double-sided ferrule which grips tubing in 

two places. This increases the holding power and decreases 

the force on one area of the tubing. A unique Wrench-Tight TM 

and Finger-Tight design is available and combines the 

convenience of a Finger-Tight TM fitting with the option to 

wrench-tighten when higher pressures are used. 

Two-Piece DSF Fitting Specifications 

Material: PEEK or Kel-F ® 


Max. Pressure: Finger-Tight: 5000psig 

Wrench-Tight: 10,000psig 



A B C 

Two-Piece DSF Fittings 

Wrench-Tight/Finger-Tight 

Part No. (A) 

best 

value 

Short Hex-Head 

Part No. (B) 

Long Hex-Head 

Part No. (C) 

Color Material Qty. 

Two-Piece DSF Fittings 

Natural PEEK 10 35753 35744 35735 

Black PEEK 10 35077 37087 37097 

Replacement Ferrules (DSF) 

Natural PEEK 10 35740 35740 35740 

Natural Kel-F ® 5 32025 32025 32025 


Wing-Nut Fittings 

• “Wing-Nut” provides Finger-Tight operation to 6000psig 

• Easier to make and break connections by hand 

• Economical two-piece design 

• PEEK or Graph-Tite construction is inert and 

biocompatible 

Two-Piece Wing-Nut Fitting Specifications 

Material: 

PEEK or Graph-Tite (Carbon-Reinforced PEEK) 





A 

Two-Piece Wing-Nut Fittings 

Graphic Color Material Qty. Part No. 

Two-Piece Wing-Nut Fittings with Ferrules 

A Black Graph-Tite † 10 32327 

B Natural PEEK 10 32326 

Replacement Ferrules 

C PEEK Ferrules PEEK 10 32335 

†Carbon-reinforced PEEK. 

B 

C 

Stainless Steel Hex-Head and Finger-Tight Fittings 

A 

B 

C 

D 

E 

F 

G 

H 

Stainless Steel Two-Piece Hex-Head Fitting Specifications 

Material: 




Thread Type: 10-32 UNF for 1/16", 6-32 UNF for 1/32" 

Typical Use: 1/16" and 1/32" o.d. Tubing Connections 

Stainless Steel Two-Piece Finger-Tight Fitting Specifications 

Material: 

300 Stainless Steel and PEEK 





Stainless Steel Two-Piece Fittings 

Graphic Description Length Qty. Part No. 

Stainless Steel Two-Piece Hex-Head Fittings 

A 1/16" Nut Short 5 206085 

B 1/16" Nut Long 5 286335 

C 1/16" Nut Extra-Long 5 306141 

— 1/32" Nut Short 10 306101 

D 1/16" Female Nut — 5 321355 

Ferrules 

E 1/16" — 5 286075 

— 1/32" — 10 30640 

Stainless Steel Two-Piece Finger-Tight Fittings 

F 1/16" Nut Short 5 321185 

G 1/16" Nut Long 5 321025 

Ferrule 

H PEEK, 1/16" — 5 321805 


113


Valco ® High-Pressure Fittings 

Valco ® Fitting Specifications 

Material: 

316 Stainless Steel, PEEK, or PTFE 

Max. Temperature: 500ºC (SS) 

100ºC (PEEK and PTFE) 

Max. Pressure: 10,000psig (SS), 

8000psig (PEEK and PTFE) 

Typical Use: High-Pressure Tubing Connections 


Valco ® Nuts and Ferrules 

5431 5697 5698 5549 

A B C 


Graphic Description Qty. Part No. 

Stainless Steel Nuts 

A 1/32" Nut 10 306101 

A 1/16" Nut 10 306121 

A 1/8" Nut 10 306151 

Stainless Steel Ferrules 

B 1/32" (Type 303) 10 30640 

B 1/16" (Type 316) 10 30646 

B 1/8" (Type 316) 10 30647 

PEEK Ferrules 

C 1/16" 10 30661 

C 1/8" 10 30662 

PTFE Ferrules 

D 1/16" 10 30671 

D 1/8" 10 30672 

Valco ® Tees and Crosses 

E 

5522 5523 

Valco ® Tees and Crosses 

Graphic Size Bore Part No. 

Tees 

E 1/32" 0.25mm 30770 

E 1/16" 0.25mm 30771 

E 1/8" 0.75mm 30773 

E 1/8" 2.0mm 30774 

Crosses 

F 1/32" 0.25mm 30780 

F 1/16" 0.75mm 30782 

F 

D 

Valco ® Unions 

G 

J 

5511 5512 

I 

5520 

5521 5550 



Internal Unions 

G 1/16" 0.25mm 30713 

G 1/16" 0.50mm 30714 

G 1/16" 0.75mm 30715 

G 1/16" 1/16" 45835 

G 1/8" 2.0mm 30718 

G 1/8" 1/8" 30719 

External Unions 

H 1/8" 2.0mm 30731 

Internal Reducing Unions 

I 1/16" to 1/32" 0.25mm 30740 

I 1/8" to 1/16" 0.25mm 30746 

I 1/8" to 1/16" 0.75mm 45850 

I 1/4" to 1/8" 1/8" 30755 

External/Internal Reducing Unions 

J 1/8" to 1/16" 1/16" 30761 

J 1/4" to 1/8" 2.0mm 30765 

J 1/4" to 1/8" 1/8" 30766 

Column Endfittings 

K 1/4" to 1/16" 2.1mm 45905 

K 1/4" to 1/16" 3.0mm 45907 


Looking for stainless 

steel tubing? 


H 

K 

5562 



High-Pressure Adapters 

Universal Direct-Connect Column Coupler 

The Universal Direct-Connect Column Coupler will join any 

two columns, regardless of fitting type. A 10-32 threaded body 

accepts any of the three Kel-F ® tips on either side. Select the 

correct tip to join columns of any fitting type. 

Waters ® Tip 

Female Tip 

Valco ® Tip 

Universal Direct-Connect Column Couplers 

Graphic Description Color Part No. 

Universal Direct-Connect Column Coupler 

A Universal Type* — 28638 

Replacement Kel-F ® Tips 

B Waters ® (“C”) Red 28004 

B Female (“B”) Black 28002 

B Valco ® Blue 28006 

*Includes one stainless steel body, two Waters ® tips, and two ferrule tips. 

Universal Direct-Connect Column Adapter 

• Double-sided adapter works with male or female 

10-32 fittings 

• Polymeric ferrule insert adjusts to any stop depth 

• Seals Finger-Tight to 5000psig 

• Inert PEEK or Graph-Tite ferrule insert 

Graph-Tite 

ferrule insert 


Body 

A 

Waters ® Tip 

Female Tip 

Valco ® Tip 

B 

Universal One-Piece Coupler 

Universal one-piece column couplers adapt to the stop depth 

of any 10-32 port. Each side of these PEEK couplers adjusts 

and bottoms out in the port to ensure that minimal dead 

volume is created. 

Color-coded sleeve 

indicates i.d. 

One-Piece Couplers 

One-Piece PEEK Couplers have a 

non-wetted color-coded Delrin ® handle. 

Stop depth of the tip is fixed, and 

provides a reliable connection when 

used with matching port configurations. 

best 

seller 

Universal One-Piece Coupler 

Description Color Part No. 

0.005" i.d. Red 336501 

0.007" i.d. Yellow 336502 

0.009" i.d. Blue 336503 

0.020" i.d. Orange 336504 

6362 

best 

value 

One-Piece Column Couplers 

Port Configuration Color Part No. 

Female Black 28191 

Waters ® Red 28192 

Male Red 28193 

Valco ® Blue 28194 

Female to Waters ® — 28196 

5441 


PEEK 

ferrule insert 

5428 

Column Coupler Kits 

Connect to 

female port 

Connect to 

male port 


5442 

1706b 

Flip adapter around to accommodate any 10-32 fitting. 

Universal Direct-Connect Column Adapter 

PEEK 

Part No. 

Graph-Tite 

Part No. 

Description 

Universal Direct-Connect Column Adapter 29242 29246 

Replacement Inserts 292405 29247 

Universal Adapter Kit* 29244 29248 

*Includes two adapters and four inserts. 

PEEK 

Column Coupler Kits* 

Description 

Part No. 


PEEK 9614 

*Includes two 10-32 UNF fittings, two ferrules, and a 5cm 1/16" o.d. x 0.010" i.d. tube. 

5856 


115


High-Pressure Adapters, Plugs, and Caps 

Stainless Steel Adapters, Plugs, and Caps 

Polymeric Adapters, Plugs, and Caps 

Stainless Steel Adapters, Plugs, and Caps Specifications 

Material: 



Max. Pressure: 

Thread Type: 

Typical Use: 

10,000psig with Stainless Steel Ferrules 

10-32 UNF 

1/16" o.d. Tubing 

Polymeric Adapters, Plugs, and Caps Specifications 

Material: 

PEEK, Delrin ® , Nylon 

Maximum Temperature: 100°C 

Maximum Pressure: 

Thread Type: 

Typical Use: 

8000psig 

10-32 UNF 



A 

C 

5476 5477 

5478 6287 

B 

D 

B 

A 

5483 

5484 5485 

C 

5479 

E 

5480 

5486 

F 

D 

5470 5471 5472 

G H I 

E 

5482 5481 

F 

Stainless Steel Adapters, Plugs, and Caps 

Graphic Description Bore Qty. Part No. 

Stainless Steel Adapters 

— Union, Female 0.003" ea 77450 

A Union, Female 0.012" ea 28627 

A Union, Waters 0.012" ea 97332 

B Union, Male 0.067" ea 10061 

C Union, Male to Female 0.067" ea 28609 

D Union, Female, Finger-Tight TM * 0.013" ea 32110 

E Bulkhead Union — ea 31505 

F Tee — ea 28629 

Stainless Steel Plugs 

G Standard (7000psig) — ea 30631 

H High-Pressure (10,000psig) — ea 30636 

Stainless Steel Cap 

I Standard — ea 30606 

*Finger-Tight TM union has PEEK ferrule, maximum pressure 8000psig, maximum 

temperature 100ºC. 

Polymeric Adapters, Plugs, and Caps 

Graphic Description Bore Qty. Part No. 

PEEK Adapters 

A Union 0.010" ea 32141 

B Tee 0.010" ea 32143 

C Cross 0.010" ea 32145 

D Bulkhead Union — ea 31504 

Delrin ® Plugs 

E Blue — 10 8872 

E Black — 10 18873 

E White — 10 18874 

E Red — 10 18875 

E Yellow — 10 18876 

E Green — 10 18877 

Nylon Caps 

F White — 10 8870 







High- and Low-Pressure Fittings 

Flange-Free Low-Pressure Fittings † 

• Convenient ferrule eliminates need for flanging 

• Compatible with all 1/4-28 UNF ports 

The Flange-Free system makes connections to 1/4-28, 

flat-bottomed ports without flanging tools. Hand-tightened 

connections produce a leak-free seal to 500psig on 1/8" o.d. 

PTFE tubing and to 1000psig on 1/16" o.d. PTFE tubing. 

Hex-head fittings are available for use with harder tubing, 

such as PEEK, Tefzel ® , or metal. 

† 

U.S. Patent #4,690,437. 

Flange-Free Fitting Specifications 

Material: 

Varies, See Price Block 


Maximum Pressure: 1/8" 500psig 

1/16" 1000psig 

Thread Type: 

1/4-28 UNF 

Typical Use: 

1/16" or 1/8" o.d. Tubing Connection 

A 

5488 5489 5490 

B 

C 

Flanged Tubing Low-Pressure Fittings 

• Use with 1/8" or 1/16" flanged tubing 

• Easily tighten without a tool 

Flanged Tubing Fitting Specifications 

Material: 

PEEK with Stainless Steel Washers 


Maximum Pressure: Limited by Tubing Strength 

Thread Type: 

1/4-28 UNF 

Typical Use: 

Low-Pressure Plumbing 

A—Finger-Tight 

Flanged Tubing Fittings, 10/pk 

5542 5543 

B—Washers 

1/16" 

Part No. 

1/8" 

Part No. 

Graphic Description Material 

Flanged Tubing Fittings (washer included) 

A Natural PEEK 37165 37175 

Replacement Washers 

B 316 SS SS 20067 20068 


Flange-Free Fittings, 10/pk 


Part No. 


Part No. 

Graphic Description Material 

Finger-Tight Nuts 

A White Delrin ® 201101 201201 

A Black Delrin ® 201122 201211 

A White, Long Delrin ® 201131 201221 

A Black, Long Delrin ® 201141 201231 

A Natural PEEK 37065 37075 

Hex-Head Nuts 

B Aluminum Metal 200911 200901 

B Stainless Steel Metal 200936 200926 

Flange-Free Ferrules 

C Ferrules PEEK 201171 201271 

C 0.3mm Bore Kel-F ® 200771 — 

C 0.8mm Bore Kel-F ® 200781 — 

C Ferrules Kel-F ® 201152 201251 

C Ferrules Tefzel ® 20240 20242 

Flange-Free Plus High-Pressure Fittings 

• Seals Finger-Tight to 5000psig 

• Connects to any standard 1/4-28 port 

• Available only for 1/16" tubing 

Flange-Free Plus Fitting Specifications 

Material: 

PEEK and Stainless Steel 


Maximum Pressure: 5000psig 

Thread Type: 

1/4-28 UNF 

Typical Use: 

1/16" o.d. Tubing Connection 

Flange-Free Plus Fittings 


Fitting System* 10 25020 


Stainless Steel Bushings 10 25022 

PEEK Ferrules 10 25021 

*Includes 10 each of fittings, bushings, and ferrules. 

6260 

Pre-Flanged PTFE Tubing 

• Chemically inert 

• Supplied with 1/4-28 nylon fittings flanged in place 

Pre-Flanged PTFE Tubing Specifications 

Material: 

PTFE 


Maximum Pressure: 0.020" i.d., 1200psig 

0.030" i.d., 900psig 

0.063" i.d., 400psig 

0.100" i.d., 300psig 

Typical Use: 


Pre-Flanged PTFE Tubing, ea 

Length o.d. i.d. Part No. 

5cm 1/16" 0.030" 20013 

1/8" 0.063" 20012 

10cm 1/16" 0.030" 20015 

1/8" 0.063" 20014 

25cm 1/16" 0.030" 20017 

1/8" 0.063" 20016 

50cm 1/16" 0.030" 20027 

1/8" 0.063" 20018 

100cm 1/16" 0.030" 20043 

1/8" 0.063" 20020 

5539 


117


High- and Low-Pressure Adapters 

PEEK Port Adapters 

INTERNAL PORT TYPE 

CPI Standard (“B”) Waters ® (“C”) 1/4-28 UNF M6 

1 2 3 

4 


EXTERNAL THREAD TYPE 

CPI Standard 

(“B”) 

Waters ® 

(“C”) 

M6 1/4-28 UNF 

A 

B 

C 

D 

Part No. 32013 

Part No. 32015 Part No. 32022 








PEEK Port Adapters 

Graphic 

Coordinate 

External 

Thread Type 

Internal 

Port Type 

Part No. 

A2 CPI Standard Waters ® Style 32015 

A3 CPI Standard 1/4-28 UNF 32022 

A4 CPI Standard M6 32065 

B3 Waters ® Style 1/4-28 UNF 32023 

B4 Waters ® Style M6 32066 

PEEK Port Adapters (continued) 

Graphic 

Coordinate 

External 

Thread Type 

Internal 

Port Type 

Part No. 

C1 1/4-28 UNF CPI Standard 32013 

C2 1/4-28 UNF Waters ® Style 32016 

C4 1/4-28 UNF M6 32067 

D1 M6 CPI Standard 32014 

D2 M6 Waters ® Style 32017 

D3 M6 1/4-28 UNF 32024 

PEEK Luer Adapters 

CPI Standard (“B”) 1/4-28 UNF M6 

1 2 3 

INTERNAL PORT TYPE 

LUER TYPE 

Male Female 

E 

F 





PEEK Luer Adapters 

Graphic 

Coordinate Luer Type 

Internal 

Port Type 

Part No. 

E1 Female Luer 10-32 Female 20132 

E2 Female Luer 1/4-28 Female 20136 

E3 Female Luer M6 Female 20138 

PEEK Luer Adapters (continued) 

Graphic 

Coordinate Luer Type 

Internal 

Port Type 

Part No. 

F1 Male Luer 10-32 Female 20133 

F2 Male Luer 1/4-28 Female 20137 

F3 Male Luer M6 Female 20139 



Low-Pressure Adapters and Plugs 


• 1/4-28 threaded ports 

• For use with Flange-Free or flanged fittings 

A 

5456 5457 

B 

Low-Pressure Adapters and Plugs Specifications 

Material: 

Varies, See Price Block 


Maximum Pressure: Limited by Fittings 

Thread Type: 

1/4-28 UNF 

1/4-28 Low-Pressure Adapters and Plugs 

Graphic Description Material Qty. Part No. 

Unions 

A Union PEEK ea 20088 

B Bulkhead Union PEEK ea 31506 

C Waters ® Pump Tubing Union † PEEK ea 590110 

Tees 

D 0.8mm Bore Tee PEEK ea 20058 

D 1.5mm Bore Tee PEEK ea 20023 

Crosses 

E 0.8mm Bore Cross PEEK ea 20059 

E 1.5mm Bore Cross PEEK ea 20025 

Male 1/4-28 Adapters 

F Male 1/4-28 to Male Luer Tefzel ® ea 20019 

G Male 1/4-28 to Female Luer Tefzel ® ea 20055 

H Male 1/4-28 to Slip-Fit* PEEK ea 28848 

I Male 1/4-28 to 1/4" o.d. Tube SS ea 20021 

J Male 1/4-28 to 1/8" o.d. Tube SS ea 20056 

K Male 1/4-28 to Female 10-32 PEEK 2 32261 

Female 1/4-28 Adapters 

L Female 1/4-28 to 1/8" MPT Brass ea 20053 

M Female 1/4-28 to Female 10-32 SS ea 20041 

N Female 1/4-28 to Male 10-32 SS ea 20057 

O Female 1/4-28 to Female 10-32 PEEK 2 32262 

P Female M6 to Female 10-32 PEEK ea 32264 

Plugs 

Q Natural PEEK 10 37185 

Q Black PEEK 10 37187 

R White Nylon 5 20050 

R White Kel-F ® 5 20089 

*Slip-Fit fits 1/16" to 5/32" i.d. tubing. 

†Slip-Fit Waters ® pump tubing to left side of union, and connect standard 1/8" or 1/16" 

o.d. tubing to right side of union. 

D 

6292 

5813 5814 

5445 5734 5448 

I 

5451 

C 

F G H 

5452 

5733 5447 5453 

K 

L 

M 

J 

E 


5454 5455 5455 

N O P 

Q 

5460 5444 

R 


119



Low-Pressure Accessories 

Easy-Flange Tool 

• Uses mechanical force to form flanges—no electricity 

or heat required 

• Forms perfect flanges on 1/16" to 1/8" o.d. PTFE or 

thin-walled PEEK tubing 

• Completely portable—use it anywhere in your lab 

Using mechanical force (no electricity or heat required), 

Easy-Flange forms perfect flanges on any PTFE tubing 

with an o.d. between 1/16" and 1/8" or thin walled (0.015" 

maximum wall thickness) PEEK tubing. Simply cut the tubing 

to the desired length, place a fitting on the tubing, slide the 

tubing into the Easy-Flange body, and form the flange using 

the flange-rolling tool. The entire operation takes just seconds 

and produces flanges with superior sealing characteristics 

compared to flanges formed by conventional heat methods. 

Low-Pressure In-Line Quick Connect 

• Make and break 1/16" or 1/8" tubing connections quickly 

and easily 

• Eliminate wear and tear on fittings and ferrules 

• Highly inert PEEK construction 

The Alltech ® Low-Pressure In-Line Quick Connect allows 

fast, effortless connection of 1/16" or 1/8" tubing where 

1/4-28 fittings are used. Made from high quality PEEK, 

the quick connect withstands repetitive connections 

and is compatible with a wide range of solvents. The 1/16" 

quick connect withstands pressures up to 1000psig, and 1/8" 

quick connect withstands pressures up to 500psig. 

Quick Connect 

5526 

6156 

Easy-Flange Tool 

Description 

Part No. 

Easy-Flange Flanging Tool* 35900 


1.5mm Pin (Polymer) 35905 


1.3mm Pin (Titanium) 35910 





Clean-Cut Tubing Cutter 35902 

Replacement Blade (for Part No. 35902) 35903 

*Includes: Clean-Cut tubing cutter and insert disks 35908, 35907, 35906, 

35910, 35920. 

Suggested Quick Connect Usage: 

• Perfect for plumbing low- and medium-pressure 

LC systems 

• Use with any low-pressure switching valve 

• Quickly swap mobile phase bottles from LC system 

or filtration equipment 

• Make and break connections to an online degasser 

Low-Pressure In-Line Quick Connect 

Description 

Part No. 

1/16" Quick Connect 77301 

1/8" Quick Connect 77302 


Looking for low-pressure tubing? 

See page 387. 


Looking for low-pressure 1/4-28 fittings? 

See page 117 for Flange-Free fittings. See page 117 for flanged 

fittings. 

5531 



Fitting and Accessory Kits 

Basic Start-Up Package 

Provides the essential products for HPLC system start-up. 

No frills and no unnecessary cost. 

best 

value 

Advanced Start-Up Package 

Keeps your HPLC consumables stocked for some time with 

products that maximize system and lab efficiency. 

best 

value 

Basic Start-Up Package 

Description Material Qty. Part No. 

Basic Start-Up Package — 1 97817 

Replacement Individual Components 

Short Hex-Head Nuts/Ferrules PEEK 10 35744 

Long Hex-Head Nuts/Ferrules PEEK 10 35735 

Short Finger-Tight One-Piece* PEEK 12 32070 

1/8" Flange-Free Nuts PEEK 10 37075 

1/8" Flange-Free Ferrules Tefzel® 10 20242 

1/16" x 0.020" i.d. Tubing PEEK 10ft 35708 


1/8" x 0.063" i.d. Tubing TFE 10ft 20063 

Plastic Tubing Cutter — 1 3206 

Replacement Blade — 1 3214 

10µm Solvent Inlet Filter SS 2 28870 

20µL Sample Loop PEEK 1 32195 

Open-End Wrench, 1/4" x 5/16" — 1 1998 

Union PEEK 1 32141 

6283 

Advanced Start-Up Package 

Description Material Qty. Part No. 

Advanced Start-Up Package — 1 97818 

Replacement Individual Components 

Short Hex-Head Nuts/Ferrules PEEK 10 35744 

Long Hex-Head Nuts/Ferrules PEEK 10 35735 

Short Finger-Tight No-Slip TM Fitting* PEEK 24 32937 

Long Finger-Tight No-Slip TM Fitting PEEK 10 32295 

1/8" Flange-Free Nuts PEEK 10 37075 

1/8" Flange-Free Ferrules Kel-F ® 10 201251 



1/8" x 0.063" i.d. Tubing TFE 10ft 20063 

Clean-Cut Tubing Cutter — 1 35902 

Replacement Blade — 2 35903 

10µm Last-Drop Inlet Filter PAT ® 2 28839 

In-Line Filter, 2µm PAT ® 1 68250 

Replacement Filter Elements PAT ® 5 68152 

20µL Flex-Connect Sample Loop PEEK 1 32195 

100µL Flex-Connect Sample Loop PEEK 1 32204 

Handy-Lok Fitting Wrench — 1 35751 

Open-End Wrench, 1/4" x 5/16" — 1 1998 

Union PEEK 2 32141 

*Assorted Colors 

6282 



Accessories for your system. Grace 

has an assortment of Accessories 

Quick Reference guides available for 

many popular instruments. 

• Waters ® Alliance ® 

System Accessories 

– Request M202 

• Agilent 1100 and 1200 



• Waters ® Acquity UPLC ® 




121


Alltech ® Fitting Kits 

• Find all common accessories in one convenient kit 

• Fittings to match your HPLC system 

• Includes both high- and low-pressure fittings 

Alltech ® Fitting Kits provide all the necessary HPLC 

accessories conveniently stored in one place. You will always 

find what you need to keep your system running. The chart 

lets you determine how many of each product are included in 

each systems fitting kits. 


Fittings Kits 

Beckman 

97137 

PerkinElmer ® 

97148 

Rheodyne ® * 

97130 

Shimadzu ® * 

97151 

Universal 

97145 

System Components Part No. Material 

Nuts 

Universal SS Male Nut 206085 316 SS 15 5 4 10 5 — 

Two-Piece Finger-Tight 32310 PEEK 2 2 2 2 — 2 

Universal Wrench-Tight/Finger-Tight 35754 PEEK 5 5 4 5 15 5 

1/16” Flange-Free (1/4-28) 201101 Delrin ® 6 6 6 — — 6 

Long Universal SS Male Nut 28633 316 SS — — 10 — — 10 

One-Piece Fitting 32232 PEEK — — — — 2 — 

Long Finger-Tight One-Piece 32234 PEEK — — — 2 — — 

Standard Wrench-Tight/Finger-Tight 35752 PEEK — — — 4 — — 

Ferrules 

Universal SS Ferrule 286075 316 SS 20 10 4 10 10 20 

Ferrule for Two-Piece Finger-Tight 32322 PEEK 4 4 2 4 — 4 

Double-Sided Ferrule 35730 PEEK 10 10 10 10 30 10 

1/16” Flange-Free Ferrule 201152 Kel-F ® 6 6 — — — 6 

Plugs 

10-32 Plug 8872 Delrin ® 2 2 — 2 — 2 

SSI Products 

Nut 01-0140 316 SS — 4 5 — — — 

Ferrule 01-0142 316 SS — 4 5 — — — 

Rheodyne ® Products 

Bushing 77510 316 SS — 4 4 4 — — 

Long Bushing 77512 316 SS — 2 2 4 — — 

Extra-long Bushing 77519 316 SS — 2 2 10 — — 

Ferrule 77511 316 SS — 10 10 10 — — 

Unions 

PEEK Female Union 32141 PEEK 1 1 3 1 3 — 

SS Male Union 10061 316 SS — — 1 — 1 — 

Waters ® Female Union 97332 316 SS — — — — — 1 

Adapters 

Universal Column Adapter 29242 PEEK/SS — — — — 1 — 

Tubing, EDM-Cut 

0.010” i.d. x 5cm 97045 316 SS 5 5 5 5 5 5 

0.010” i.d. x 10cm 97055 316 SS 5 5 5 5 5 5 

0.010” i.d. x 20cm 97060 316 SS 5 5 5 5 5 5 

0.020” i.d. x 5cm 97075 316 SS 5 5 5 5 5 5 

0.020” i.d. x 10cm 97080 316 SS 5 5 5 5 5 5 

0.020” i.d. x 20cm 97085 316 SS 5 5 5 5 5 5 

*Can also be used for Spectra-Physics ® . 

Waters ® 

97155 

6261 



Mobile Phase Inlet Filters 

Cup-Style Inlet Filters 

• Disposable filters and reusable PTFE sleeve for 

long-term savings 

• Uses all the mobile phase in the reservoir 

Cup Filters use all the mobile phase in the reservoir without 

drawing air into the system. A replaceable stainless steel filter 

cup provides the same high surface area of conventional 

mobile phase filters. But unlike conventional filters, a PTFE 

sleeve fits over the cup filter so mobile phase is drawn from 

the bottom of the bottle. 

Cup-Style Inlet Filter Specifications 

Material: 

PEEK, PTFE, 316 Stainless Steel 

Porosity: 2µm or 10µm 


Plumbing: 

Slip Fit to 1.5, 2.2, or 3.0mm i.d. Tubing 

Uni-Fit Slip fits to 

1.5, 2.2, or 3.0mm i.d. 

Tubing 

Replaceable Stainless 

Steel Cup Filter 

Reusable 

PTFE Sleeve 

6265 

Economy Inlet Filters 

• Flat bottom design draws all solvent from the reservoir 

• Economical style means economical price 

Economy Filters have 22mm stainless steel filter that sits 

on the bottom of the mobile phase reservoir drawing in all but 

a few milliliters of mobile phase. The heavy PTFE housing 

acts as a “sinker“ and has a 1/8" port to push tubing securely 

in place. 

Economy Inlet Filter Specifications 

Material: 

PTFE, Stainless Steel 



Plumbing: 

Push Fit for 1/8" o.d. Tubing 

hplc accessories | mobile phase 

6337 

Cup-Style Inlet Filters 

Description Porosity Qty. Part No. 

Stainless Steel Cup Filter 2µm 1 28981 

Stainless Steel Cup Filter 10µm 1 28982 

Replacement Cup Filter 2µm 1 28983 

Replacement Cup Filter 10µm 1 28984 

Economy Inlet Filters 


Stainless Steel Filter 2µm 1 79964 

Stainless Steel Filter 10µm 1 79966 

Hastelloy ® C Inlet Filter 

• Ideal for use with halogenated solvents 

• More inert than other types of 

stainless steel 

CPI Stainless Steel Inlet Filter 

• Unique knurled fitting connects securely to 

1/8" o.d. tubing 

• Comes with replaceable Tefzel ® ferrule 

5216 

6297 

Hastelloy ® C Inlet Filter Specifications 

Material: 

Hastelloy ® C 

Porosity: 2µm 


Plumbing: 

Slip Fit to 1.5 or 3.0mm i.d. Tubing 

Hastelloy ® C Inlet Filters 


For 0.062" (1.5mm) i.d. 2µm ea 9400 

For 0.125" (3.0mm) i.d. 2µm ea 9402 

CPI Stainless Steel Inlet Filter Specifications 

Material: 316 Stainless Steel, Tefzel ® 



Plumbing: 

CPI Port for 1/8" o.d. Tubing 

CPI Stainless Steel Inlet Filters 


Stainless Steel Filter 10µm ea 250145 

Replacement Filter 10µm 2 250147 

Replacement Tefzel ® Ferrules — 5 210144 


123


Mobile Phase Inlet Filters 

Metal-Free Inlet Filter 

• Ideal for IC or biochromatography applications 

• Replaceable polyethylene filter elements 

• Threaded body allows easy connection to 1/4-28 fittings 

Stainless Steel Slip-On Inlet Filter 

• Economical mobile phase inlet filtration 

• 316 stainless steel filters 

best 

seller 


Metal-Free Inlet Filter Specifications 

Material: 

PEEK Body, Polyethylene Filter 



Plumbing: 

1/4-28 Port for 1/16" or 1/8" o.d. Tubing 

Metal-Free Inlet Filters 


For 1/8" o.d. 20µm ea 32170 

For 1/16" o.d. 20µm ea 32171 

Replacement Filter Elements 20µm 5 32173 

5217 5564 

Stainless Steel Slip-On Inlet Filter Specifications 

Material: 




Plumbing: 

Slip Fit to 1.5, 2.2, or 3.0mm i.d. Tubing 

Stainless Steel Slip-On Inlet Filters 


For 0.062" (1.5mm) i.d. 2µm 5 28861 

For 0.062" (1.5mm) i.d. 10µm 5 28863 

For 0.085" (2.2mm) i.d. 2µm 5 28865 

For 0.085" (2.2mm) i.d. 10µm 5 28867 

For 0.125" (3.0mm) i.d. 2µm 5 28869 

For 0.125" (3.0mm) i.d. 10µm 5 28871 

tech tip 

How do I choose a mobile phase inlet filter? 

Choose the filter material based on the analysis 

Stainless Steel: Suitable for most applications except very strong acid or halogenated 

solvents 

Hastelloy ® C: More inert grade of stainless steel, suitable for halogenated solvents 

where halide salts tend to corrode other types of stainless steel 

Polyethylene: Good general inertness. Ideal for any metal-free or IC application. 

Choose the filter porosity based on the HPLC flow rate 

Flow Rate (mL/min) 

Inlet Filter Porosity 

0.1–5 2µm 

1–10 10µm 

10–50 20µm 


Looking for low-pressure tubing? 

Grace carries a full line of low-pressure 

tubing. See page 387. 


Looking for mobile 

phase degassers? 


5531 

6508a 







Mobile Phase Delivery/Storage-Bottle Kits 

Kontes Three-Valve Bottle Kit 

These bottle kits have three color-coded, 

helium-tight valves to maintain a sealed 

environment between runs. Each kit comes 

complete with a three-valve cap, a safetycoated 

conical bottom Kontes Ultra-Ware ® 

reservoir, and the required 1/4-28 fittings 

and tubing. 

Kontes Three-Valve Bottle Kit 

Description Thread Size Part No. 

Three-Valve Bottle Kit 

1 Liter GL-45 31870 

2 Liter GL-45 31871 

5 Liter GL-45 31872 

THF-Resistant Three-Valve Bottle Kit 

1 Liter GL-45 31875 

2 Liter GL-45 31876 

5 Liter GL-45 31877 

Kontes Three-Hole Bottle Kit 

The standard Three-Hole bottle kit includes 

a safety-coated conical-bottom Kontes Ultra- 

Ware ® reservoir and a Three-Hole cap with 

1/4-28 threads on both the top and bottom 

portions of the cap to separate tubing lines 

inside and outside the reservoir. 

Kontes Three-Hole Bottle Kit 


Three-Hole Bottle Kit 

1 Liter GL-45 31880 

2 Liter GL-45 31881 

5 Liter GL-45 31882 

Kontes Economy Three-Hole Bottle Kit 

The Economy Three-Hole Bottle Kit 

includes a safety-coated flat-bottom 

Kontes Ultra-Ware reservoir and an 

Economy Three-Hole cap with 1/4-28 

threads on the top portion of the cap 

(2 x 1/8" and 1 x 1/16" through hole). 

Pass a single length of tubing through 

the cap into the reservoir. 

best 

value 

1842 

2226 

Kontes Safety-Coated Glass Bottles 

• Plastic coating blocks UV light and retains glass 

fragments if breakage occurs 

• Safety pressure rating of 6psig 

• Conical bottom reservoir delivers all mobile phase 

Kontes Ultra-Ware ® reservoirs are made from the most 

chemically inert glass available to prevent leaching 

extractables into the mobile phase solvent. In case of an 

accident, the external safety coating retains glass fragments 

and serves as a UV blocking agent up to 385nm. 

Conical Bottom 

5574 

Flat Bottom 

Kontes Safety-Coated Bottles 


Kontes Ultra-Ware ® Conical Bottom Reservoir 

1 Liter GL-45 10133 

2 Liter GL-45 10135 

5 Liter GL-45 10137 

Kontes Ultra-Ware ® Flat-Bottom Reservoir 

1 Liter GL-45 10967 

2 Liter GL-45 10968 

5 Liter GL-45 10969 

tech tip 

Tips for safe helium sparging 

As with any glass container, a nick or score caused by normal 

lab usage will lessen the integrity of the glass, so beware in any 

pressure or vacuum application. Ideally, use safety-coated glass 

bottles, and perform vacuum filtration and/or helium sparging 

under a protective hood. 

5577 


1841 

Kontes Economy Three-Hole Bottle Kit 


Economy Three-Hole Bottle Kit 

1 Liter GL-45 95393012 

2 Liter GL-45 95393022 

5 Liter GL-45 95393052 

10 Liter GL-45 95393013 

20 Liter GL-45 95393023 


125



Mobile Phase Delivery/Storage Bottles 

Glass Mobile Phase Bottles 

• Economical 

• Graduated markings 

• Borosilicate glass 

Economical glass mobile phase 

bottles with un-lined storage caps 

are available in 500mL, 1000mL, 

and 2000mL sizes. These graduated 

borosilicate glass bottles can be 

ordered individually or by the case. 

Glass Mobile Phase Bottles 

Volume Thread Size Qty. Part No. 

500mL 33-430 1 2198191 

500mL 33-430 6 219816 

1000mL 38-430 1 2198201 

1000mL 38-430 6 219826 

1000mL GL-45 1 2199301 

1000mL GL-45 6 2193011 

2000mL GL-45 1 2199311 

2000mL GL-45 6 219311 

Kontes Mobile Phase Caps 

• Sparge and deliver solvent with one cap 

• Available with or without valves 

Kontes Three-Valve Cap has three 1/4-28 threaded ports 

and color-coded, helium-tight valves to maintain a sealed 

environment. The Standard Three-Hole Cap has 1/4-28 

threads on the top and bottom portions of the cap for separate 

tubing lines inside and outside the reservoir. The Economy 

Three-Hole Cap has 1/4-28 threads on the top portion of 

the cap (2 x 1/8" and 1 x 1/16" through holes). Pass a single 

length of tubing through the cap into the reservoir. 

Three-Hole Cap 

best 

value 

5588 5573 

Three-Valve Cap 

Kontes Mobile Phase Caps 


Three Valve Cap GL-45 10140 

Standard Three-Hole Cap GL-45 10974 

Economy Three-Hole Cap GL-45 10973 

TFE-Storage Cap, No Holes GL-45 10153 

5595 

Alltech ® Economy Mobile Phase Caps 

• Most economical way to helium sparge 

and deliver HPLC mobile phases 

• Three-hole cap with or without threaded ports 

The MP1-Cap has a PTFE insert and a polypropylene screw 

collar. The insert contains three 1/4-28 threaded ports with 

through-holes* that allow the tubing to either be pushed 

through or connected with flanged fittings. The MP2-Cap 

is made from Tefzel ® and polypropylene and has three 

unthreaded holes* for tubing insertion. Just push the tubing 

through! Alltech also offers PTFE lined screw caps with 

one or two pre-drilled holes (not shown). 

*(2x1/8" and 1x1/16") 

MP1-Cap 

6338 6339 

Alltech ® Economy Mobile Phase Caps* 

Description Qty. Thread Size Part No. 

MP1-Cap ea GL-45 955664 

MP2-Cap ea GL-45 955665 

1-Hole Screw Cap 4/pk 38-430 9547 

2-Hole Screw Cap 4/pk 38-430 9541 

*Not intended for build-up of helium environment. 

Omnifit ® Mobile Phase Caps 

• Anti-twist design prevents 

tubing from getting tangled 

• Highly inert PTFE wetted 

surface 

• Stainless steel inserts prevent 

cross-threading 

MP2-Cap 

best 

value 

Omnifit ® bottle caps are made 

6334 

from a single PTFE block built into 

a Polypropylene threaded cap. The anti-twist design enables 

the central block to turn independently when the cap is being 

connected onto the bottle. This prevents tubing from being 

twisted. The 1/4-28 ports have reinforced non-wetted stainless 

steel threaded inserts. Optional Kel-F ® turn-key valves provide 

a completely closed system. 

Omnifit ® Mobile Phase Caps 


Two-Hole Cap GL-45 53280 

Three-Hole Cap GL-45 53281 

Two-Hole Cap with Valve GL-45 53282 

Three-Hole Cap with Valve GL-45 53283 



Kontes Ultra-Ware ® Filtration 

Cap System 

Alltech ® Solvent Filtration 

Apparatus 

Now you can filter, degas, and 

deliver mobile phase from the same 

reservoir with this modular system. The 

Filtration/Degassing Cap uses a 47mm 

diameter membrane to filter directly 

into the reservoir. Vacuum degassing 

is accomplished by closing the Hi-Vac 

valve on the Solvent Pickup Adapter. 

This system comes complete with the 

Filtration/Degassing Cap, a standard 

Solvent Pickup Adapter, a Conical 

Bottom Kontes Ultra-Ware ® Reservoir, 

and the required fittings and tubing. 

Conical Bottom 

Reservoir 

A 

Solvent Pick-Up 

Adapter 

0860 2765 

0861 

B 

Filtration/ 

Degassing Cap 

C 

5575 

The Alltech ® Solvent Filtration Apparatus 

rapidly filters and degasses HPLC 

and IC mobile phases. Depending on 

your analytical requirements, several 

interchangeable funnel and flask options 

are available. You can easily replace 

the funnel with the solvent pickup 

adapter. The Solvent Pickup Adapter 

draws solvent directly from a reagent 

bottle through a 47mm filter and into the 

collecting flask. 

Glass Funnel 

Clamp 

Filter of Choice 

Solvent 

Pickup 

Adapter 

6266 


CTFE Adapter 

Vacuum Adapter 

Support Frit 

Fritted Glass 

Support Base 

Solvent 

Bottle 

D 

0866 0867 

2770 

E 

F 

125mL/1L 

2L 

2771 

Kontes Ultra-Ware ® Filtration Systems 

Graphic Description Part No. 

Filtration Cap System 

— With 1 Liter Reservoir 31860 



Replacement Parts and Accessories 

A 1 Liter Reservoir* 10133 



B Solvent Pickup Adapter (THF Resistant) 31869 

C Filtration/Degassing Cap 10141 

D CTFE, 1/4-28 to 1/8" Barb Adapter 10128 

D CTFE, 1/4-28 to 5/32" Barb Adapter 10139 

E Vacuum Adapter 1/4-28 to 1/4" i.d. Tubing 10127 

F Replacement Support Frit 31801 

*Bottles have GL-45 threads. 

Alltech ® Solvent Filtration Apparatus 

Description 

Part No. 

Solvent Filtration Apparatus 

125mL Flask, 15mL Funnel, 25mm Filter Support, 

2331 

25mm Clamp, 0.2µm Nylon Filters 100/pk 

1L Flask, 300mL Funnel, 47mm Filter Support, 

2001 


2L Flask, 500mL Funnel, 47mm Filter Support, 

2003 



125mL Flask (For 2331) 2337 

1L Flask (For 2001) 350062 

2L Flask (For 2003) 350066 

15mL Glass Funnel (For 2331) 2333 



1L Glass Funnel (for 2003) 350070 

25mm Fritted Base Support (For 2331) 2335 



25mm Aluminum Clamp (For 2331) 2339 

47mm Aluminum Clamp (For 2001, 2003) 350061 

47mm Solvent Pick Up Adapter 2343 

47mm Nylon Filter Membranes, 0.2µm, 100/pk 2034 

25mm Nylon Filter Membranes, 0.2µm, 100/pk 2050 


127



Alltech ® On-Line Degassing Systems 

• Most advanced degassing technology 

• Product range meets every need and budget 

• Upgrade capabilities available 

Flow rate instability and baseline noise are the likely result 

of dissolved air in the mobile phase. Eliminate these problems 

with the Alltech ® On-line Degassing System. Unlike helium 

sparging, degassing online does not require extra mobile 

phase preparation steps. Save time and avoid the expense, 

inconvenience, and hazards associated with helium tanks. 

Every Alltech ® On-Line Degassing System is equipped with our best degassing technology, and is available in two families, 

three flow ranges, and four channels to meet every need and budget. First decide which family features meet your need, then 

choose the degassing system with the flow range and number of channels you need today. If your needs change in the future 

you can upgrade at any time by either replacing or adding up to four degassing channels per unit. 

Advanced Degassing Technology from Three Key Components 

Teflon ® AF Degassing Membrane 

50x more efficient than competitive PTFE membranes. 

This means channel volumes are 10x smaller and 

equilibration is 4x faster. 

UV Absorbance @ 215nm 

0.300 

0.250 

0.200 

0.150 

0.100 

0.050 

Teflon ® AF Degassing Membrane 

PTFE Membrane 

Air saturated Methanol @ 1.0mL/min 

0.000 

0 200 400 600 800 1000 1200 1400 s 

Time Required to Reach Optimum Degassing Level 

Continuous-Run Vacuum Pump 

Delivers a steady baseline. Competitive degassers 

have fluctuating baselines due to the on/off cycle of the 

vacuum pump. 

UV Absorbance @ 215nm 

0.100 

0.090 

0.080 

0.070 

0.060 

0 

Baseline Using Alltech ® Degassing System 

Baseline Using Competitor’s Degassing System 

Air saturated Methanol @ 1.0mL/min 

200 400 600 

800 1000 1200 1400 s 

5768 5769 

6508a 

Low-permeation Tubing 

Double-layered with an inert PTFE inner layer and a gas 

impermeable outer layer so solvent remains degassed as 

it travels to the pump. 

Oxygen (ppm) / Meter of Tubing 

1.0 

0.8 

0.6 

0.4 

0.2 

0 

PTFE Tubing 

1/8" OD x 1/16" ID 

Regassing Rate 

@ 1mL/min. 


Standard PTFE Tubing 


1/8" OD x 1/16" ID 

1911B 



Alltech ® Online Degassing Systems 

Degassing System 

HPLC Pump 

Detector 


Injector 

HPLC Column 

Mobile Phase 

Eliminate extra mobile phase preparation steps by degassing online. 

6524 

Choice of Two Degasser Families: 

Elite Degasser Family 

In addition to the key degassing components, the Elite 

family incorporates a vacuum sensor, validation output and 

a four-year warranty. The sensor not only detects and alerts 

you to leaks, it also communicates with the vacuum pump. If 

a change in vacuum is detected due to mobile phase flow rate 

changes, the pump can compensate by changing its speed. 

The validation output records vacuum level to satisfy ISO and 

system validation requirements. 

Specification Elite Degasser Family Select Degasser Family 

Membrane Type: Teflon ® AF Teflon ® AF 

Vacuum Pump Type: Continuous-run, self-adjusting speed Continuous-run, one-speed 

Low-permeation Fittings and Tubing: Yes Yes 

Vacuum Sensor: Yes No 

Validation Output: Yes (5mVDC/1mm Hg) No 

Warranty: 4-year 1-year 

Flow Range Choices: 

Micro: 

Analytical: 

Semi-prep: 

0–1mL/min, 195µL internal volume 



Select Degasser Family 

The Select family has identical degassing efficiency to 

the Elite line since it contains the same key components. 

However, by omitting the vacuum sensor and validation 

output, and applying the industry standard one-year warranty, 

the Select family is available at an economical price. 




Universal Power Supply: Yes Yes 

Dimensions: 5" H x 3" W x 10" D (13cm H x 8cm W x 25cm D) 5" H x 3" W x 10" D (13cm H x 8cm W x 25cm D) 


On-Line Degassing Systems 

Description 

Elite Family 

Part No. 

Select Family 

Part No. 

Micro, 0–1mL/min, 195µL Internal Volume 

2 Channel 590507 590500 

4 Channel 590508 590501 

Analytical, 0–5mL/min, 480µL Internal Volume 

1 Channel 590509 590502 

2 Channel 590510 590503 

3 Channel 590511 590504 

4 Channel 590512 590505 

Semi-prep, 0–10mL/min, 925µL Internal Volume 

1 Channel 590517 590514 

2 Channel 590513 590506 

3 Channel 590518 590515 

4 Channel 590519 590516 





Channel Upgrade Kits* 

Description 

Part No. 

Micro, 0–1mL 590520 

Analytical, 0–5mL 590521 

Semi-prep, 0–10mL 590522 

*Includes degassing channel, vacuum tubing, mounting screw, identification sticker, 

and instructions. 

Face Plates* 

Description 

Part No. 

For 1 Channel 

590506A 

For 2 Channel 

590506B 

For 3 Channel 

590506C 

For 4 Channel 

590506D 

*To replace an existing degassing channel, only a channel upgrade kit is necessary. 

To add more channels, a new face plate is also required. 

Upgrade Labor Charge* 

Description 

Part No. 

Labor 590525 

*Channel upgrade kit and face plate must be ordered separately. 


129



Helium Sparger Kit 

• 38-430 threaded cap 

• Available in single, binary, or 

ternary versions 

The Alltech ® Helium Sparger 

kit has a 316 stainless steel 

sparging element which may be 

immersed in the mobile phase 

reservoir. A length of 1/8" PTFE 

tubing connects the sparger to 

a flow-control valve (optional) 

which connects to a source of 

helium, and allows the bubble 

rate to be adjusted. A 38mm, 

two-hole cap attaches to the 

top of standard 1-liter mobile 

phase reservoirs or to any 4-liter 

solvent bottle. All kits include 

tubing, reservoir cap(s), and sparging element(s). For kits with 

a flow control valve, one valve controls the bubble rate on all 

sparging outlets. 

Ultrasonic Bath 

• For laboratory cleaning, 

solvent degassing, and 

dispersing slurries 

• 2.8-liter tank 

• Mechanical timer 

• Overall size: 

12" H x 13.3" W x 11.6" L 

• Tank Size: 

4.0" H x 5.5" W x 9.5" L 

Flow Control Valve 

38mm 

Cap 

PTFE Tubing 

Sparging 

Element 

Helium Sparger Kits 

Description 

Part No. 

Single Sparger Kit with Flow Control Valve 28915 

Binary Sparger Kit with Flow Control Valve 28919 

Ternary Sparger Kit with Flow Control Valve 28923 

Replacement Sparging Element 28862 

Ultrasonic Bath 

Description 

Part No. 

120V 9490 

220V 9490-220V 

tech tip 

Use caution when filtering 

As with any vacuum or pressure application, glassware cannot 

be guaranteed against breakage due to varying conditions of 

field usage. Be sure to filter in a glass hood. Safety-coated glass 

bottles are always recommeneded. 

0881 

5602 

Vacuum Filtration 

System 

47mm 

Filter 

Membrane 

GL-45 

Threads 

Filter Funnel System 

Interchange Filter and 

In-Line Cap 

Base 

4391 

47mm 

Filter 

Membrane 

GL-45 

Threads 

best 

seller 

In-Line Filtration System 

new 

• Eliminate solvent transfer steps with direct filtration into 

any GL-45 reservoir 

• Interchange funnels and in-line filter cap handle any 

filtration volume 

• Durable polypropylene 

construction 

New Features: 

• Stainless steel 

connection at vacuum 

source 

• Retainer ring prevents 

filter from twisting during 

assembly 

• A sealing o-ring in the 

neck of the GL-45 for a tighter seal 

Stainless 

Steel 

Connect 

Vacuum 

Source 

Stainless 

Steel 

Connect 

Vacuum 

Source 

Base 

Alltech ® Vacuum Filtration Systems 


1L Filter Funnel System 

1 6409 

Includes: Base, Adapter Kit, Mesh Filter Support, 

1L Funnel 

2L Filter Funnel System 

1 6429 


2L Funnel 

In-Line Filtration System 

1 6407 


In-Line Filter Cap 


1L Funnel 1 6473 

In-Line Filter Cap 1 6463 

Hose Barb Adapter Kit (includes eight adapters) 1 6438 

Stainless Steel Mesh Filter Support Ring 1 6421 

Silicone Rubber O-Ring 1 8619230 

Retainer Ring 1 8619231 

4385 



Alltech ® Adjustable 

Backpressure Regulators 

• Constant backpressure, even with changing flow 

• Ultra-low dead volume—under 0.6µL 

• Easily adjusts by hand—no cartridges 

best 

seller 

Optimize Fixed Backpressure 

Regulators 

• Flow-through design—use in-line or as a terminator 

• May be immersed in the waste solvent bottle 

• 1/4-28 ports for tubing connections 

The Alltech ® Backpressure Regulator prevents bubbles from 

forming in detector flow cells by exerting a constant pressure. 

The pressure will not vary with mobile phase composition or 

flow rate. Optimized port configuration delivers an internal 

volume of less than 0.6µL. A convenient, knurled knob adjusts 

the pressure, and eliminates cartridge changes. It also allows 

adjustments to be made without interrupting flow. 

Alltech ® Adjustable Backpressure Regulators 

Description 

Part No. 

0–100psig 39021 

0–300psig 39022 

100–600psig 39023 

100–1500psig 39024 

4704 

These flow-through, backpressure regulators prevent solvent 

outgassing in your LC detector. More than a flow restrictor, 

Optimize fixed regulators maintain a consistent backpressure, 

regardless of viscosity or flow rate. Pressure ratings are 

preset to their listed value. Internal volume is 100 microliters. 

They are supplied with fittings, and complete instructions. 

Optimize Fixed Backpressure Regulators 

Description 

Part No. 

10psig 29059 

30psig 29060 

60psig 29062 

100psig 29064 

150psig 29069 

5625 


Alltech ® Liquid Pressure Gauges 

• Inert 316 stainless steel 

construction 

• Built-in 1/16" connecting tee 

for in-line use 

• Non-wetted glycerine-filled 

face plate 

These liquid gauges come 

equipped with a 10-32 threaded, 

1/16" connecting tee for attaching it in-line to the 

solvent stream. Each gauge is constructed of 316 stainless 

steel and features an easy-to-read 2-1/2" face plate. 

2349 


For high-pressure fittings, 

see pages 112–118. 

6280 

Alltech ® Pressure Gauges 

Description 

Part No. 

0–100psig 19255 

0–300psig 19256 

0–1000psig 19257 

0–3000psig 19258 

0–5000psig 19259 


For PEEK tubing, see 

pages 384–385. 

5535 


131


hplc accessories | pump 

Optimize Pump Replacement Parts 

OPTI-MAX ® Cartridge Check Valves 

• Economical, disposable cartridge with reusable holder 

• Available with PEEK or stainless steel cartridges 

• Same cartridge fits inlet 

and outlet housings 

• Cartridge fits multiple 

housing brands for 

reduced inventory 

OPTI-MAX ® Check Valve Assembly (Holder and Cartridge) 

Description 

Inlet 

Part No. 

Outlet 

Part No. 

Standard Assembly (1/8" Ball) For: 

Agilent/HP 1050, 1100 155231* 155232 

Bio-Rad ® 1330 32350 32351 

Dionex ® GPM-2,DXP, AGP Analytical, 32354 32356 

2000, 4000 

Gilson ® /Rainin ® 5, 10, 25mL S, SC Heads 155511 155521 

PerkinElmer ® Inlet Model 250, Series 4, 32358 32359 

200, 400, 410, 620, Integral 4000 

Spectra-Physics ® 8100, 8700, 8800 155441 155452 

Series, P-Series 

Waters ® M45, M45G, M501, 6K, 6KA, 155001 155111 

510, 590, 600E, 610 

Low Flow Rate Assembly (1/16" Ball) For: 

Hitachi ® 655, L6000, L6200, L6200A 155341 155352 

Hitachi ® L-7100 (LaChrom) 155233 155234 

Shimadzu ® LC-10 AT, LC-10ATVP 155235 155236 

Shimadzu ® LC-10AD, LC-600, LC-9A 155237 152238 

Waters ® 2690 Alliance, 616 155250 155251 

Waters ® 625, 626 155252 155253 

*Replace cartridge for active inlet valve. 

2376 

OPTI-MAX ® Replacement Cartridges 


Standard Series (1/8" Ball) 

PEEK 2 151002 

316 Stainless Steel 2 10481 

Low Flow Rate Series (1/16" Ball) 

PEEK 2 10482 

316 Stainless Steel 2 10483 

Optimize Pump Pistons 

• Pump pistons meet or exceed manufacturer’s standards. 

Optimize Pistons 

Manufacturer 

Part No. 

Grace 

Part No. 

Description 

Pump Pistons For: 

Agilent/HP 1050, 1100 5062-2441 11907 

Agilent/HP 1090 3980-0672 11908 

Beckman ® 110A, 110B, 112, 112M 243053 11901 

Dionex ® Analytical 36904 11904 

Hitachi ® 655, L6000, L6200/A, 7100 635-1021 11909 

(LaChrom) 

LDC, Milton Roy ® 801306 980014 

Shimadzu ® LC-6A LC-1A, LC-10AS, 228-12904-93 11912 

LC-10AT, LC-10ATVP 

Shimadzu ® LC-600/LC-9A, LC-10AD 228-18523-91 11913 

Spectra-Physics ® 8800, 8810, P-1000, A3102-010 11914 

P-2000, P-4000 

Waters ® M510, M590, M600E, M610, 25656 11915 

M6000, M6000A 

Waters ® 2690 Alliance 270959 119165 

OPTI-SEAL ® Pump Seals 

• Ideal for aqueous solutions 

• Proprietary UHMW-PE blend, OS-10.1 

ITB Improved PTFE-Blend Seals 

• Ideal for high organic solutions 

or when broad solvent 

compatibility is required 

• Proprietary PTFE blend 

2379 

OPTI-SEAL ® Pump Seals 

2378 

Manufacturer 

Part No. 

OPTI-SEAL ® 

Part No. 

ITB Seal 

Part No. 

Manufacturer 

Description 

Qty. 

Agilent/HP 1050, 1100 5062-8516 ea 32056 11883 

Bio-Rad 1350, 2700 1250302 ea 32050 11875 

Hitachi 655, L6000, L6200, L6200A, L-7100, LaChrom ® 655-1080 10 25649 11886 

ea 25648 11887 

LDC Milton Roy 206129001 10 25643 11889 

Shimadzu LC-6A, LC-7A, LC-10AS, LC-10AT, LC-10ATVP 228-11999-00 10 25645 11893 

ea 25644 11894 

LC-600/LC-9A, LC-10AD 228-18745-00 10 32063 — 

ea 32062 11896 

Spectra-Physics 8800, 8810, P-1000, P-2000, P-4000 A2963-010 10 11864 — 

ea 11865 11898 

Waters M45, M45G, M501, M510, M590, M600E, 22934 10 25639 11899 

M610, M6000, M6000A ea 25638 11866 

Waters 2690 Alliance ® 26938 10 119166 — 

ea 119167 — 



ASI Pump Replacement Parts 

ASI Cartridge Check Valves 

• Self-priming 

• Rugged construction 

• Convenient and 

economical 

• Superior flow stability 

The ASI cartridge has a 

15,000psig pressure limit. 

During routine maintenance, replace only the cartridge, 

not the entire assembly. Cartridges cost less than most 

conventional valve rebuild kits. 

Every valve is tested prior to shipping to ensure it passes 

a stringent set of performance specifications. A replaceable 

2µm outlet filter insures clog-free operation and protects the 

column from pump wear particles. 

ASI Check Valve Assembly (Holder and Cartridge) 

Pump Model 

Inlet 

Part No. 

Outlet 

Part No. 

Assembly For: 

Beckman ® 110, 112, 114, 116, 118, 125, 15811 15831 

126, 127, 128 

Gilson ® 5 and 10s, SC 15815 15835 

Hitachi ® 655, L6000, L6200, L6200A 15817 15837 

LKB 2150 15820 — 

Shimadzu ® LC600, LC6A, LC7A, LC9A, 15823 15843 

LC10A, LC10AS, LC10AT 

Spectra-Physics ® 8100, 8700, 8700, 8800, 15824 15844 

8810 

Waters ® M45, 510, 590, 6000 15828 15848 

Waters ® M501 and 600E 15828 15850 

Waters ® 6KEF, 6KAEF, 510EF, 600EF 15829 15849 

ASI Replacement Cartridges 

Inlet 

Part No. 

Outlet 

Part No. 

Pump Model 

ASI Replacement Cartridges For: 

Beckman ® 110, 112, 114, 116, 118 125, 126, 15854 16123 

127, 128 

Bio Rad ® 1330 and 1350 — 16124 

Gilson ® 5 and 10s, SC 15858 16127 

Hewlett-Packard ® 1090 15859 16128 

PerkinElmer ® Series 4, 200, 400, 410, 620, 15864 16133 

4000 

Shimadzu ® LC600, LC6A, LC7A, LC9A, 15866 16135 

LC10A, LC10AS, LC10AT 

Spectra-Physics ® 8100, 8700, 8800, 8810 15867 16136 

Waters ® M45, 501, 510, 590, 600E, 6000 16120 16139 

2240 

ASI Pump Pistons 

ASI offers precisionmanufactured 

replacement 

pump pistons. Superior 

surface finish of the piston 

rods improves piston seal 

lifetime. 

ASI Pistons 

ASI Pump Seals 

• Replacement seals for 

a variety of pumps 

• All seals below are 

UHMW-PE, unless 

otherwise specified 

Manufacturer 

Part No. 


Part No. 

Description 

Pump Pistons For: 

Gilson ® 10, SC 3650009 11746 

Gilson ® 5SC 3650008 11747 

Hitachi ® 655, L6000, L6200, L6200A, 810-1033 11760 

L7100 

Shimadzu ® LC-600, LC-9A, LC-10AD 228-18523-91 11766 

Spectra-Physics ® 8800, 8100 A3102-010 11767 

Waters ® M-45, 501 26524 11364 

Waters ® M-510, 590, 600, 6000 25656 11365 

Waters ® Ext. Flow 510EF, 590EF, 600EF, 

610EF, 650EF, 6000EF, 6000AEF 

60304 11366 

ASI Pump Seals 

Manufacturer 

Part No. 


Part No. 

Description 

Qty. 

Pump Seals For: 

Agilent 1090—PTFE ea 5062-2494 11679 

Agilent 1090—PTFE 10 11680 

Gilson ® /Rainin ® 5SC ea 5463088863 11675 

Gilson ® /Rainin ® 5SC 10 11676 

Hitachi ® 655, L6000, L6200, ea 655-1080 11681 

L6200A, L7100 

Hitachi ® 655, L6000, L6200, 10 11682 

L6200A, L7100 

Spectra-Physics ® 8800, 8100 ea A2962-010 11687 

Spectra-Physics ® 8800, 8100 10 11688 

Waters ® M45, 501, 510, 590, ea 22934 11695 

600, 6000 

Waters ® M45, 501, 510, 590, 10 11696 

600, 6000 

Waters ® M45, 501, 510, 590, ea 26613 11697 

600, 6000—PTFE 

Waters ® Ext. Flow UHMW ea 26644 11699 

2334 

2334 













• Waters ® Acquity ® 




133



PEEK Mixing Tee 

• Better mixing than a 

conventional tee 

• Inert, metal-free construction 

• Ideal for high-pressure gradient 

mixing 

Better mixing is the result of the 

5616 

turbulence generated by the angled ports and the flow 

through a PAT (PEEK alloyed with TFE) filter element. Solvent 

streams mix more thoroughly than with a conventional tee. 

This reduces baseline noise and improves high pressure 

gradient mixing or post-column reactions. Solvents contact 

PEEK and the PAT filter, providing a biocompatible, inert, and 

metal-free flow path. This tee has a 2.5µL internal volume, 

a pressure limit of 6000psig, and comes with fittings and 

ferrules. 

Fitting 

Double-Sided 

Ferrule 

Toroid Mixer 

PAT Filter 

PEEK Housing 

• High efficiency mixer for preparative 

chromatography 

• Precise and reliable 

Turbulence at 

Vertex Mixes 

Two Solvent 

Streams 

Peek Mixing Tee 


Mixing Tee with Fittings ea 32157 

Replacement PEEK Fittings 5 35752 

Replacement PEEK Ferrules 5 35730 

The unique design of the Toroid Mixer 

extends the mixing characteristics of 

a pulse dampener in higher flow rate 

applications. High efficiency mixing yields reproducible 

quantitation, better reproducibility of retention times, and 

reduced baseline noise due to uniform solvent composition. 

Featuring a clean-swept mixing chamber, the Toroid Mixer 

has no motors or moving parts. 

Toroid Mixer Specifications 

Internal Volume: 5mL 

Flow Range: 

10 to 200mL/min 


Flow Path: 

PEEK 

1024 

4708 

ASI Static Mixer 

• Interchangeable mixer cartridges 

• Selectable volume* 

• Suitable for analytical and micro 

LC flow rates 

ASI HyperShear Static Mixers use a 

highly efficient liquid-liquid shearing 

interface which induces vortex mixing 

over a wide flow rate range. The 

versatile cartridge design is available as an in-line, binary, 

or ternary inlet housing. Analytical volume housings accept 

350 or 500µL mixer cartridges. Low volume housings accept 

50, 150, or 250µL mixer cartridges. And Micro volume 

housings accept 2, 5, 10, or 25µL mixer cartridges. All are 

rated to 5000psig. 

ASI HyperShear Static Mixers 

SS 

Part No. 

PEEK 

Part No. 

Description 

Analytical Volume Housing 

In-Line 40167 — 

Binary Inlet 40168 — 

Ternary Inlet 40169 — 

Analytical Volume Mixer Cartridges 

350µL 40146 — 

500µL 40147 — 

Low Volume Housings 

In-line 20141 201411 

Binary Inlet 20143 201431 

Ternary Inlet 20145 201451 

Low Volume Mixer Cartridges 

50µL 20147 201471 

150µL 20148 201481 

250µL 20149 201491 

Micro Volume Housing 

In-Line 30167 — 

Binary Inlet 30168 — 

Ternary Inlet 30169 — 

Micro Volume Mixer Cartridges 

2µL 30146 — 

5µL 30147 — 

10µL 30148 — 

25µL 30166 — 

*Other cartridge volumes available, call for assistance. 

tech tip 

What should I consider when choosing a mixer volume? 

• For any given flow rate, the more mixing volume, the better 

the mixing and lower the baseline noise. 

• The smaller the mixing volume, the better the definition and 

sharpness of step gradients. 

• High-pressure multi-pump gradient systems typically require 

far less mixing volume than low-pressure single-pump 

gradient systems. 

4707 

Toroid Mixer 

Description 

Part No. 

Toroid Mixer, Biocompatible 060143 



Hamilton ® Priming Syringe 

• Ideal for use with Waters ® pumps 

Hamilton ® Priming Syringe 

Description 

Part No. 

10mL GasTight Priming Syringe 81662 

Draw Needles for Waters ® Pump 25559 

OPTI-PRIME Valve 

• Convenient—onehanded 

operation 

• Efficient—no need 

to remove syringe 

• Inert—PPS, TFE, and 

316SS wetted surfaces 

The syringe of the 

OPTI-PRIME serves 

as a handle to open the 

OPTI-PRIME valve, 

allowing one-handed operation. At a 45° angle, air bubbles 

rise to the top of the syringe, allowing purging and priming 

without removing the syringe. 

1896 

1898 

5506 

Valve open 

at 45º to 

prime pump 

Valve 

closed 

OPTI-PRIME Valve 

Description 

Part No. 

OPTI-PRIME Priming Valve 11647 

OPTI-PRIME Priming Valve w/Safety Syringe 11648 

Optimize Safety Syringe 11649 

Pump Inlet Kit 

Install the Pump Inlet Kit between 

the mobile phase reservoir 

and the pump to allow rapid 

priming and solvent changeover. 

A luer-lock adapter in the side 

port easily attaches to a priming 

syringe. Includes: one 3-way 

valve, one luer adapter, 2µm inlet 

filter, 100cm x 1/8" o.d. x 1/16" 

TFE tubing, two 1/8" flange-free 

fittings and ferrules. 

Pump Inlet Kit 

Description 

Part No. 

Pump Inlet Kit 39652 

Replacement Inlet Filter 9400 

LO-Pulse Dampener 

• 95% pulse reduction 

• Only 900µL internal 

volume 

As a pressure pulse enters 

the dampener, the flexible 

diaphragm transfers the 

pulse to the compressible 

fluid resulting in a 95+% 

reduction in pressure 

fluctuation. The low internalvolume 

(only 900µL at 6000psig) and flow-through design 

minimize gradient delays. 

LO-Pulse Dampener Specifications 

Flow Path Material: 

PEEK or 316 Stainless Steel 

Internal Volume: 

0.9mL 

Workable Pressure Range: 500–6000psig 


Dampener Mechanism: Compressible Fluid 

5617 

2350 


In-Line Bubble Trap 

This handy accessory 

installs on pump inlet lines 

by slip fitting onto the 

connecting tubing. Bubbles 

in the solvent line are 

trapped before reaching 

the pump. Includes 

mounting bracket. 

Slip fits to 3.0, 

2.2 or 1.5mm 

i.d. tubing 

LO-Pulse Dampener 

Description 

Part No. 

Stainless Steel 20-0218 

PEEK 120312 

Diaphragm Replacement Kit 120139 


Need a liquid pressure gauge? 

See page 131 for a complete selection. 

2349 

In-Line Bubble Trap 

Description 

Part No. 

In-Line Bubble Trap 01-0221 

4042 


135


hplc accessories | valves 

Rheodyne ® Model 7725 and 9725 Analytical Injectors 

• “Make-Before-Break” (MBB) switching for 

uninterrupted flow 

• Wider port angles provide improved fitting access 

• Position sensing switch option 

• Capable of injecting volumes from 1µL to 5mL with 

high precision 

• Suction Needle Adapter eliminates exposure of sample 

to metal of a syringe needle 

The 7725i features a built-in position-sensing switch, providing 

the chromatograph with a reproducible start signal. The 9725, 

9725i are biocompatible versions. Wetted materials are PEEK 

and Tefzel ® , making them ideal for applications requiring a 

metal-free flow path. 

Rheodyne ® Model 7125 Analytical Injectors 

• Versatile and reliable reputation 

• 5µL to 5mL sample loops available 

• Position-sensing switch option 

• The wetted materials are: Titanium, Tefzel ® , and ceramic 

Flow Diagram 

Analytical Sample Injectors 

Description Version Part No. 

7725, Includes a 20µL PEEK Sample Loop SS 77250 

7725i, Includes a 20µL PEEK Sample Loop SS 77251 

9725, Includes a 20µL PEEK Sample Loop PEEK 40010 

9725i, Includes a 20µL PEEK Sample Loop PEEK 40011 

Accessories 

Suction Needle Adapter — 40016 

5503 

Waste 

6 

1 

5 

2 

4 

Pump 3 

Column 

Waste 

6 

Sample In 1 

5 

Sample In 

2 

4 

Pump 3 

Column 

Position A (LOAD) Position B (INJECT) 

5502 



7125 SS 7125RV 

Accessories 

Suction Needle Adapter — 40016 

5500 

Rheodyne ® Model 7010 and 9010 Analytical Injectors 

• Rear-loading valves 

• High volumetric precision 

• Low volume flow passages 

• Loop-Filler Port accommodates needles from 0.018" to 

0.028" diameter 

The 7010 injects sample volumes of 5µL or larger by means 

of a removable sample loop. Functionally similar to the 7010, 

the 9010 is more inert, made from PEEK, Tefzel ® and ceramic. 

The 9010 comes with a built-in position-sensing switch. 

Loop Filler Port 

Accessory 



7010, Includes a 20µL Sample Loop SS 7010RV 

9010, Includes a 20µL Sample Loop PEEK 9000RV 

Accessories 

Loop-Filler Port SS 7044 

Loop-Filler Port PEEK 40012 

Needle Port Fitting — 7012-009 

5501 



Rheodyne ® Model 8125 

Micro-Scale Injector 

• Versatile!—Works with microbore (1–2mm i.d.) and 

analytical (2–6mm i.d.) columns 

• Low dispersion 0.25mm flow passages 

• Built-in position-sensing switch 

Rheodyne ® Model 7410 

Micro-Scale Injector 

• High volumetric precision 

• 0.5µL to 2µL removable internal sample loops 

• Designed for 1–2mm i.d. columns 

The 8125 valve has unique “dual size” ports for tubing 

connections. This allows either 1/16" o.d. or 0.02" o.d. tubing 

to be used. Connections to 1/16" column endfittings are 

facilitated with a connecting tube supplied with the valve. 

The smaller flow passages also result in more resistance to 

flow. This makes it harder to load large sample volumes. The 

maximum recommended sample volume for the 8125 valve is 

500µL. All sample loops are externally plumbed. 

5498 5499 

Rheodyne ® Model 7410 Sample Injection Valve is a single 

mode injector whereby sample is loaded with the complete 

filling method using a loop-filler port. This injector can be used 

with Rheodyne ® loop-filler port accessories, position sensing 

switches, and mounting panels. 

The valve has four ports which are threaded to accept 

Rheodyne ® male nuts and ferrules (compression-type tube 

fittings) for 1/16" o.d. stainless steel tubing. 


Micro-Scale Sample Injector 


8125 SS 8125RV 

Micro-Scale Sample Injector 


7410 SS 7410RV 

Accessories 

0.5µL Loop Disc — 7410-070 

1µL Loop Disc — 7410-071 

2µL Loop Disc — 7410-072 


Need sample loops and fittings for your 

Rheodyne ® injector? 

For sample loops, see page 140. 

For fittings, see page 141. 


Did you know that Grace has a complete offering of nano, 

capillary, and microbore columns for both small and 

large molecule applications? See page 31 for explanation 

of hardware formats and see pages 24 and 25 for phase 

descriptions. 

5640 

6950 





6951 


137


Rheodyne ® Manual Switching Valves 

• Inexpensive manual switching valve 

• Wide-bore version for prep 

Manual Switching Valve Applications 

2-Position 6-Port: 

Two Column Selection 

2-Position 6-Port: 

Pre-Column Backflushing 

Injector 

Injector 


Rheodyne ® HPLC Switching Valves offer an endless variety of 

ways to simplify switching and injecting. Standard valves are 

supplied with 0.024" internal flow passages and are suitable 

for use with analytical and semi-preparative applications. 

Large bore valves with 0.040" internal flow passages are 

available for use at high flow rates (greater than 50mL/min) 

7000psig pressure limit. 

A spring detent mechanism included in the 6-position 

selection valve permits full circle rotation in either direction 

and ensures that the rotor “falls into” each of the six positions 

at the precise 60° spacing. 

*5000psig pressure rating for 2-position 6-port and 10-port. 

5619 

6 

1 

5 4 

Detector 

2 

3 

0798 

Off line column is sealed. Flow 

continues on line. 

3-Way Valve: 

Column Programming 

Analytical Column 

6 

1 

2 

5 4 

3 

Detector 

Pre Column 

0801 

Flow continues through column. 

Only pre-column is backflushed. 

4-Way Valve: 

Column Backflushing 


Description Version Bore Part No. 

2-Position Valves 

6-Port SS Standard 7000RV 

6-Port SS Large Bore 7000L 

3-Way SS Standard 7030RV 

3-Way SS Large Bore 7030L 


Multi-Position Valves 

6-Position 6-Port SS Standard 7060RV 

Column B 

Column A 

6 

1 

2 

5 4 

Detector 

3 

Injector 

Column C 

6 

1 

2 

5 4 

Detector 

3 

Injector 

0802 

0800 


Need fittings for valves? See page 141. 

Part of sample is analyzed on 

columns A-B, the other part on 

columns A-C. 

Flow is backflushed to direct 

peak of interest to detector. 

6-Position 6-Port Valve: 

Tandem Five Column Selection 

Detector 

5 

4 

6 

3 

1 

2 

5 

4 

6 

3 

1 

2 

Injector 

Bypass Flush-Out Line 

Selects between five columns. Use the sixth port for a bypass/flush 

out line. Two 7060s are required. 

0799 



Rheodyne ® RheBuild ® Kits 

for Injection Valves 

• Contains everything necessary for valve maintenance 

• Conveniently packaged according to valve model 

• Economical—Save over buying individual replacement 

parts 

Rheodyne ® Replacement 

Parts 

Knob Assembly 

Set Screws (2) 

Needle Guide 

Shaft Assembly 

Body 

RheBuild ® Kits contain a complete assortment of all the parts 

you need for general valve maintenance. In addition to the 

genuine Rheodyne ® parts selected for your specific valve, 

detailed instructions guide you through the repair process. 

5777 

Spring Washers (4) 

Needle Port Tube Assembly 

Isolation Seal 

Thrust Bearing 

Rotor Pin 

Seal Pins (4) 

Bearing Ring 

Rotor Seal (Vespel) 

Stop Pins (2) 

Stator Ring 

Stator Face Assembly 

Stator Set Screws (3) 

Stator 

Stator Screws 

0818 


Rheodyne ® RheBuild ® Kit Specifications 

Model 

Includes: 

3725, 3725i, 3725-038, 3725i-038, Standard Rotor Seal, Stator Face 

7125, 7126, 7125-081, 7725, 7725i, Assembly, Isolation Seal, Needle 

7726, 8125, 8126, 9125, 9126, 9725, Guide, Needle Port Cleaner, 2 Hex 

9725i, 9726 

Keys, and Instructions 

7520, 7526 Inlet Stator, Rotor, Needle Guide, 

Needle Port Cleaner, Hex Key, Rotor 

Removal Tool, and Instructions 

7010, 7000, 7410, 9010, 9040 Standard Rotor Seal, Isolation Seal, 

2 Hex Keys, and Instructions 

Rheodyne ® RheBuild ® Kits 

Description 

Part No. 

3725, 3725i, 3725-038, 3725i-038 10455 

7125, 7126 10457 

7125-081 10459 

7725, 7725i, 7726 10461 

8125, 8126 10463 

9125, 9126 10465 

9725, 9725i, 9726 10467 

7520, 7526 10469 

7010, 7000 10471 

7410 10473 

9010, 9040 10474 

Replacement Parts for Rheodyne ® Injectors 


Isolation Seal for All Valves ea 7010-015 

Stator Screw for 7010 10 77514 

Rotor Seal for 7010 ea 7010-039 

Stator for 7010/7125 ea 7010-040 

Stator for 7060 ea 7060-039 

Needle Guide for 7125/7725/7725i/8125/9125 ea 7125-008 

Knob Assembly for 7125 ea 7125-043 

Rotor Seal for 7125/7126/7725/7725i ea 7125-047 

PEEK Rotor Seal for 9010 ea 9010065 

Tefzel ® Rotor Seal for 9010 ea 90100510 

Stator Face Assembly for 7125 ea 7125-067 



Stator for 7725/7725i ea 7725010 

Stator Face Assembly for 7725/7725i ea 77250260 

Rotor Seal (Vespel) for 8125 ea 8125038 



Stator for 9125 ea 9125-036* 

Tefzel ® Rotor Seal for 9125 ea 9125-082 

PEEK Rotor Seal for 9125 ea 9125-095 

Tefzel ® Rotor Seal for 9010 ea 9010065 

*Includes ring. 


139



Rheodyne ® Sample Loops 

Metal Sample Loops 

• Square cut and burr free 

tubing for flush connection 

to the valve 

• Includes unswaged fittings 

and ferrules 

• Coiled and configured for 

specific valves 

Rheodyne ® metal sample loops 

are available in 316 stainless steel 

or titanium. Actual volumes can differ due to the tolerances of 

the tubing. Precise volumes may be injected by the “partialfilling” 

method if you are using a dual-mode injector. 

Metal-Free Sample Loops 

• PEEK construction is inert and biocompatible 

• Flexible tubing makes them 

easy to use 

• Includes versatile RheFlex ® 

fittings 

PEEK sample loops are a 

convenient alternative to 

stainless steel. Actual sample 

loop volume can differ from 

theoretical designations due to 

the ±0.05mm tolerances on the 

tubing bore. 

Sample Loops—Analytical Injectors 

Metal 

7725/7725i 

Part No. 

Metal 

7125/7010 

Part No. 

Metal 

8125/8126 

Part No. 

PEEK 

9000/9725 

Part No. 

Description 

2µL (Internal) 7725015 — — — 

5µL 7725020 70-20SL 802001 9055020 

10µL 7725021 70-21SL — 9055021 

20µL 7725022 70-22SL — 9055022 

50µL 7725023 70-23SL — 9055023 

100µL 7725024 70-24SL 7725024 9055024 

200µL 7725025 70-25SL 7725025 9055025 

500µL 7725026 70-26SL 7725026 9055026 

1mL 7725027 70-27SL 7725027 9055027 

2mL 7725028 70-28SL 7725028 9055028 

5mL 7725029 70-29SL 7725029 9055029 

5641 

2745 

5640 


The Handy-Lok Fitting Wrench 

has a slotted design which 

fits conveniently around the 

connecting tubing, and grips the 

entire hex securely. This gives 

you better access to fittings 

in awkward or obstructed 

locations, such as the back 

of an injection valve. 

See page 393 to order the 

Handy-Lok Fitting Wrench. 

Alltech ® Sample Loops 

Flex-Connect Sample Loops 

• Flexible coil bends into place 

quickly and easily 

• Includes universal fittings that 

prevent dead volume 

• 100% PEEK construction— 

prevents corrosion and sample loss 

Two-piece Ferrule Does Not 

Twist Tubing 

PEEK Flex-Connect Sample Loops 


Flex-Connect Sample Loop with Hex-Head Nuts and a Two-piece Ferrule 

5µL ea 32194 

10µL ea 32196 

20µL ea 32195 

50µL ea 32202 

100µL ea 32204 

200µL ea 32206 

500µL ea 32210 

1mL ea 32222 

Fittings (PEEK) 

Short Hex-Head Nut and Ferrule 5 32215 

Long Hex-Head Nut and Ferrule 5 32217 

Two-Piece Ferrule 5 32218 

1086 

Stainless Steel Sample Loops 

• Includes finger-tight fittings that 

require no wrenches 

• PEEK ferrule automatically adjusts 

to port dimensions 

• Leak-proof to 5000psig 

• Stainless steel loop, PEEK ferrule 

Flexible Coil 



5µL Sample Loops ea 32101 






Sample Loop Kit 

Includes: 1 each of above Loops, 

ea 32125 

12 PEEK Ferrules 

Fittings 

Long Stainless Steel Nut 5 321025 

Short Stainless Steel Nut 5 321185 

PEEK Ferrules 5 321805 

0813 

5642 



Rheodyne ® ChromTRAC 

Fitting System 

• Allows hex-head fittings to be finger-tightened 

• Color code fluid lines 

• Fits tightly atop any Alltech ® or Rheodyne ® hex-head 

10-32 fitting 

ChromTRAC ID Kit 


ID Kit* 20/pk 6002830 

*Four each of blue, red, yellow; two each of grey, green, white, black. 

Rheodyne ® RheFlex ® PEEK Fittings with ChromTRAC 

One-Piece 

Part No. 

Two-Piece 

Part No. 

5379 

Two-Piece 

Extra-Long 

Part No. 

Color 

Qty. 

Qty. 

Qty. 

RheFlex ® Fittings with ChromTRAC 

Blue 10 6002821 5 6005401 — — 

Grey 10 6002822 5 6005402 1 6006602 

White 10 6002823 5 6005403 1 6006603 

Green 10 6002824 5 6005404 1 6006604 

Red 10 6002825 5 6005405 1 6006605 

Yellow 10 6002826 5 6005406 1 6006606 

Black — — 5 6000054 1 6006608 

Multicolor* 10 6002827 — — — — 

*Includes two each of blue, grey, green, red, yellow. 

Valve Mounting Brackets 

Rheodyne ® Mounting Panels 

Aluminum mounting plates are drilled 

with the appropriate holes for attaching 

all Rheodyne ® high-pressure valves. A 

hole is included for the Model 7012 Loop- 

Filler Port. The flat panel (1.57 x 5.12", 

4 x 13cm) has valve mounting holes at 

one end and panel mounting holes at the 

other. The angled panel (2.5 x 2.5 x 2.3", 

6.4 x 6.4 x 5.9cm) is shown above. 

Rheodyne ® Valve Mounting Panels 

Description 

Part No. 

Mounting Panel 

7160RV 

Angle Mounting Bracket 7160-010 

Alltech ® Ring Stand Brackets 

These sturdy brackets allow 

Rheodyne ® or Valco ® HPLC 

valves to be securely mounted 

to standard laboratory ring 

stands. The dimensions for 

the mounting bracket are 

1.5 x 2.5 x 4.5" (3.8 x 6.4 x 11.4cm). 

Alltech ® Ring Stand Brackets for HPLC Valves 

Description 

Part No. 

Mounting Bracket 9540 

tech tip 

What Is the correct way to plumb a valve? 

5623 

5624 


Rheodyne ® RheFlex ® Fittings 

A 

E 

B 

H 

0808,1886-1888 

Rheodyne ® RheFlex ® Fittings 


RheFlex ® Stainless Steel Fittings with Ferrules 

A Nut 10 77510 

B Long Nut 10 77512 

C Extra Long Nut 10 77519 

D Replacement Ferrules 10 77511 

RheFlex ® PEEK Fittings with Ferrules 

E RheFlex ® Fitting 5 6000054 

F Short RheFlex ® Fitting 5 6000055 

G Replacement Ferrules 5 6000051 

RheFlex ® PEEK One-Piece Fingertights 

H RheFlex ® Fitting 10 600282 

F 

C 

D 

G 

Figure 1 

1. Make a burr-free square cut on any tubing that will be plumbed 

into the valve. This ensures the tubing will “bottom out” in 

the valve port. If tubing does not “bottom out”, a cavity will 

form which leads to peak distortion such as fronting, tailing 

or broadening. 

2. Place a nut and ferrule on the tubing 

3. Insert tubing into the valve port. Confirm that the tubing is 

bottomed in the valve port as shown in Figure 1. 

4. While firmly pressing the tubing, finger-tighten the nut as much 

as possible. For hex-head fittings, use a wrench to turn the 

fitting another 1/4 to 1/2 turn past finger-tight. 

0629 


141



Alltech ® SelectPRO Automated Switching Valves 

• Increase productivity 

• Save time with unattended operation 

• Available in stainless steel or PEEK 

SelectPRO valve systems are electronically-controlled 

instruments. They increase productivity and save time and 

money by eliminating repetitive, manual switching. Now you 

can automate a wide variety of fluid-processing applications 

such as methods development, solvent switching, sample 

preconcentration, and fraction collection. 

SelectPRO valve position is always indicated by a digital 

display on the front panel. Operation is easy with manual 

switching on the front panel or automated switching with 

a built-in electronic communication interface. Automated 

control through the communication interface is accomplished 

by either level logic (2-position) or four-line BCD (multiposition) 

control. All line controls require contact closure 

or TTL outputs. 

SelectPRO Automated Switching Valves Specifications 

Dimensions: 

5.5" H x 3.0" W x 7.5" D 

(14cm H x 76cm W x 19.1cm D) 


5000psig (345 bar)* 

Warranty: 

1 Year 

2-Postion Valve Control: 

Level Logic 

Multi-Position Valve Control: 4-Line BCD 

Supplied With: 

Interface Cable and Universal Power 

Supply 

*Maximum pressure for 6-position solvent selector is limited by 1/4-28 fittings. 

SelectPRO TM Switching Valve Applications 

2-Postition, 10-Port: On-line sample enrichment to 

decrease total analysis time 

2-Channel Selector with By-Pass: Reduce equilibration 

time during solvent changeover between two columns 

4108 

Alltech ® SelectPRO Automated Switching Valves 



6-Port SS 85521 

10-Port SS 85531 

6-Port PEEK 85525 

10-Port PEEK 85535 


2-Channel Selector By-Pass SS 85563 

2-Channel Selector By-Pass PEEK 85567 

10-Position Selector SS 85583 

10-Position Selector PEEK 85587 

6-Position Solvent Selector, 1/16" † SS 85593 

6-Position Solvent Selector, 1/8" † SS 85597 

SelectPRO Valve RheBuild ® Kits 

For 2-Position, 6-Port** 85529 

For 2-Position, 10-Port** 85539 

For 2-Channel Selector with By-Pass** 85569 

For 10-Position Selectors** 85589 

For 1/16" Tubing, 6-Position Solvent 

85594 

Selectors 

For 1/8" Tubing, 6-Position Solvent Selectors 85599 

**Suitable for Stainless Steel or PEEK version. 

† 

Low pressure valve uses 1/4-28 fittings. 

10-Position Selector: Select ten columns using two 

valves to accelerate methods development 

6-Position Solvent Selector: Select various mobile 

phase compositions for method development. 

4107 

4109 


Refer to page 141 for Rheodyne ® fittings. 

4110 



Alltech ® SelectPRO Column Selector 

• Streamline method development and validation 

• Screen multiple HPLC columns quickly 

and automatically 

• Eliminate sample carry-over and minimize dispersion with 

low dead-volume valve architecture 

The Alltech ® SelectPRO TM Column Selector is a self-contained, 

electrically operated instrument for automated column 

selection. A SelectPRO TM Column Selector is a powerful tool 

for quickly screening multiple columns for the best separation. 

After column selection and method optimization is achieved, 

use the column selector to quickly validate methods using 

different lots of the same packing material or, alternatively, 

different columns from the same lot. 

Many laboratories have a number of users sharing the 

same system. The column selector lets multiple users select 

methods using the correct column for their assay and offers 

convenient washout and column storage. Different samples 

can be run at night and over weekends using different 

methods, better utilizing existing equipment and increasing 

productivity. 

The selected column is indicated by a bright digital display 

on the front panel. Select columns remotely using 4-line BCD 

electronic interface, or locally by push button. 

Design Features: 

• Pinch clips simplify column mounting and removal 

• Built-in drip tray provides safe and convenient solvent 

containment for leaks and spills 

• Magnetically secured cover maintains environmental 

isolation and stabilizes ambient temperature 

• Side tubing fasteners organize tubing connections 

• Accommodates columns in lengths of 3–35cm 

(2–14") and diameters of 0.6–3.2cm (0.25–1.25") 

Column Selector 

Pump 

1.00 

Column 5 

Column 6 

4534 4535 

Column 4 

Column 3 

6 

Sample Injector 

Column 2 

In 

Column 1 

4 

5 

Column Selector 

with Cover 

3 

2 

1 

Out 

1 ı 3ı 5 ı 

Detector 

2 ı 6 ı 

4 ı 


SelectPRO Column Selector Specifications 

Dimensions: 

22" H x 9.8" W x 12.8" D 

(55.9cm H x 24.9cm W x 32.5cm D) 

Maximum Pressure: 5000psig (345 bar) 

Flow Passage: 0.6mm (0.024") and 0.4mm (0.015") 

Environment: 

Supplied with: 

Warranty: 

Control: 

4–40°C,


ASI QuickSplit Flow Splitters 

Post-Column Fixed Splitters 

Post-Column Adjustable Splitters 


• Easy-to-use “plug and play” device 

• Predefined split ratio eliminates tedious 

adjustments to capillary tubing 

• Ultra-low dead volume as small 

as 100nL 

• Interchangeable fluid resistors create 

split ratios from 1:1 to 2000:1 

ASI Post-Column Fixed Flow Splitters provide a fixed 

split ratio with extremely low dead volume—as low as 

100 nanoliters, depending upon the resistor cartridge. 

Split ratios are unaffected by solvent viscosity or pressure 

changes, and are extremely stable and reproducible. Ultra 

low internal volume means that solvent composition in both 

resistors at the same time is identical, and therefore viscosity 

changes associated with gradient runs do not impact the 

split ratio. These splitters have an input flow range from 

0.1 to 5mL/min. 

Post-Column Fixed Flow Splitters and Replacement Resistor Sets 

Splitter 

Part No. 

Replacement Resistor Set 

Part No. 

Ratio 

2000:1 620103 6200103 

1000:1 620104 6200104 

500:1 620105 6200105 

200:1 620106 6200106 

100:1 620107 6200107 

50:1 620108 6200108 

20:1 620109 6200109 

10:1 620010 6200110 

5:1 620011 6200111 

3:1 620012 6200112 

1:1 620013 6200113 

6793 

• Adjustable flow splitter enables direct 

real time control over split ratio 

optimization 

• Create split ratios from 1:1 to 1000:1 

• Ultra low 0.5µL delay volume on 

capillary side 

• Easy to use interchangeable fluid 

resistors create a wide range of split ratios 

The flow path of the ASI post-column Adjustable Flow Splitter 

contains two fluid resistors that form a parallel flow path. The 

low flow rate stream passes through a fixed resistor cartridge, 

while the high flow rate stream passes through an adjustable 

fluid resistor (metering valve). The ratio of these two resistors 

creates the split flow ratio. Due to the rugged design, the split 

ratio repeatability is ±1% of setting, and unlike alternative 

splitter valves or tees, will not be affected by actions that 

effect input flow such as turning the pump off and on, or 

pressure spikes. 

Because the Adjustable Flow Splitter incorporates a 

metering valve, split ratios can be changed frequently with 

flow changes that are stable and reproducible. This provides 

direct real time control over split ratio optimization. Split ratios 

are not affected by changes in solvent viscosity or pressure. 

These splitters have an input flow range from 0.1 to 5mL/min. 

ASI Post-Column Adjustable Flow Splitters 

Description 

Part No. 

ASI Post-Column Adjustable Flow Splitters 

Adjustable from 50:1 to 1000:1 60201 




Replacement Resistor Cartridges 

For 50:1 to 1000:1 601101 

For 15:1 to 250:1 601103 

For 5:1 to 100:1 601104 

For 1:1 to 20:1 601106 

6328 

ASI QuickSplit Makeup-Flow Splitter Manifold 

• Add makeup-flow to predetermined post column splits 

• Built-in delay time ensures proper sequencing between 

detector and fraction collector 

• Extremely low peak dispersion 

This splitter manifold is designed for post-column applications 

where a small amount of flow from an HPLC column is 

combined with a makeup-flow before it reaches the detector. 

A common application that uses this device involves splitting 

a small portion of the outlet flow from a preparative HPLC 

column which is then combined and diluted with a makeupflow. 

This flow is sent to a mass spec or UV to trigger a 

fraction collector from the remaining flow. 

Makeup-Flow Splitter Manifold 

Flow Range Split Ratio Part No. 

1–20mL/min 1000:1 620014 

20–50mL/min 10,000:1 620015 

50–150mL/min 5000:1 620016 

Replacement Resistor Cartridges 

1–20mL/min 1000:1 620017 

20–50mL/min 10,000:1 620018 

50–150mL/min 5000:1 620019 



Hamilton ® Miniature Inert 

Low-Pressure Valves 

• Inexpensive low-pressure injection valves 

• Convenient solvent selection 

• Variety of flow configurations 

• Available in standard or panel mount 

Hamilton ® low-pressure valves provide an economical and 

flexible method of custom-building a fluid flow control system 

from a simple on/off valve to more complex multi-valve 

arrangement. The small valve body and inert flow path is 

suited for any low-pressure system. Three body styles are 

available. The HV is a stand alone manual valve. The HVP is 

the same as the HV valve except that it is panel mountable. 

The HVX is panel mountable and has a larger internal 

diameter of high flow or viscous fluids. 

5505 5668 5669 

HV Valve HVP Valve HVX Valve 

Hamilton ® Miniature Inert Valves Specifications 

Material: 

PTFE, Kel-F ® flow path 

Temperature Range: 10–100ºC 


Thread Type: 1/4-28 

Port Orifice: 

0.059" for HV and HVP. 0.118" for HVX 

Hamilton ® Plug Types 

90, 180, T, 4-Port: Flow path connects two or more side ports 

Distribution: 

Flow path connects bottom port to any one side 

port 

Injection: 

Flow path connects pairs of adjacent ports 


5597 

5599 

5600 

5601 

5603 

5604 

5605 

5606 

A 

B C D 

E F G H 

5607 5608 5609 5610 

5611 

5612 

5613 

I J 

K 

L 

M N O P 

5614 

Hamilton ® Miniature Inert Valves 

Graphic Housing Type Plug Type HV Part No. HVP Part No. HVX Part No. 

A 180° 180° 86725 86775* — 

B 90° 90° 86726 86776* — 

C “T” “T” 86727 86777* — 

D “T” 90° 86728 86778* — 

E 4-Port 4-Port 86729 86779* — 

F 4-Port “T” 86730 86780* — 

G 4-Port 90° 86731 86781* — 

H 4-Port 180° 86732 86782* — 

I 180° Distribution 86735 86785* — 

J 90° Distribution 86736 86786* — 

K “T” Distribution 86737 86787* — 

L 4-Port Distribution 86738 86788* — 

M 6-Port Distribution — — 86915 † 

N 6-Port Injection — — 86916 † 

O 8-Port Distribution — — 86918 † 

P 8-Port Injection — — 86919 † 

*HVP Valves require locking nut (Part No. 16450) for panel mounting. 

† 

HVX Valves require locking nut (Part No. 35121) for panel mounting. 

Valve Accessories 

Description 

Part No. 

Panel Mount Nut for HVP Valves 16450 

Panel Mount Nut for HVX Valves 35121 

Valve Adapters 

Description Material Part No. 

1/4-28 Male Thread to Hose Barb CTFE 350320 

1/4-28 Male Thread to Male Luer CTFE 35030 

1/4-28 Male Thread to Female Luer Lock CTFE 350310 

1/4-28 Male Thread to Male Luer Lock CTFE 350330 

1/4-28 Male Thread to RN Needle Adapter CTFE 350350 


145


SSI High-Pressure Needle Valves 

• Useful to aid in HPLC pump functions 

• Available in two-way and three-way configurations 

• Higher pressure limitations than other valves 

Fittings/Ferrules 


1/16" Gland Nut 10 01-0140 

1/16" Ferrule 10 01-0142 

Two-Way Valves 

Three-Way Valve 


A 

0778 1763 

Two-Way Valve Specifications 

Material: 

PTFE, Tefzel ® , and 316 Stainless Steel 

Max Temperature: 100ºC 

Max Pressure: 

15,000psig 

Thread Type: 

1/16" SSI Fittings 

Typical Use: 

Basic shut-off valve with straight or 

angled ports 

B 

1 

3 

C 

2 3 

1 

1764 

Three-Way Valve Specifications 

Material: 

PTFE, Tefzel ® , and 316 Stainless Steel 


Max Pressure: 

15,000psig 

Thread Type: 


Typical Use: 

Pressure venting to aid in pump priming, 

purging, or solvent changeover 

2 

D 

0780 

Two-Way Valves 


A Two-Way, Through Valve 02-0120 

B Two-Way, Angle Valve 02-0121 

Three-Way Valves 


C Three-Way, Bottom Vent 02-0124 

D Three-Way, Side Vent 02-0125 

Check Valves 

Prime/Purge Valves 

0781 

FLOW 

G 

E 

0782 

F 

0779 

Check Valve Specifications 

Material: 

PTFE, Chemraz ® , and 316 Stainless Steel 


Max Pressure: 

6000psig 

Thread Type: 


Internal Volume 121µL 

Typical Use: 

Install as a back-up to inlet and outlet pump 

check valves to reduce pulsations in less 

sophisticated reciprocating pumps 

Check Valves 


G Check Valve 02-0129 

— Replacement Check Valve Seal 90-1001 

Prime/Purge Valve Specifications 

Material: 

PEEK or 316 Stainless Steel 


Max Pressure: 

6000psig 

Thread Type: 

Standard: 1/16" SSI fittings and 1/4-28 for 

low pressure side port 

Luer: 1/16" SSI fittings, and luer stem for 

low-pressure side port 

Typical Use: 

Low-pressure side port opens to prime and 

closes for normal use 

Prime/Purge Valves 

Graphic Description Material Part No. 

F Prime/Purge Valve, Luer Stem PEEK 060115 

F Prime/Purge Valve, Luer Stem SS 02-0292 

E Prime/Purge Valve, Standard SS 02-0191 

— Seal Kit for 02-0191 — 02-0194 


Prep | Flash | TLC 

Preparative Chromatography . . . . . . . . 148–183 

Introduction. . . . . . . . . . . . . . . . . . . . . . 148–151 

Columns . . . . . . . . . . . . . . . . . . . . . . . 152–171 

GraceAlpha Columns 152–155 

Vydac ® MS Columns 156–157 

Vydac ® TP Columns 158–160 

Alltech ® Columns 161 

Column Hardware . . . . . . . . . . . . . . . . . . 162–171 

MODcol ® Spring Columns 162–165 

Axial SFC Column Hardware 166–169 

Empty MODcol ® Flanged Columns 170 

Process Scale Columns (Peak Biotech) 171 

Column Packing . . . . . . . . . . . . . . . . . . . 172–174 

MODcol ® Multipacker ® Packing Stations 172–173 

Column Packing Services 174 

Media . . . . . . . . . . . . . . . . . . . . . . . . 175–181 

Introduction 175 

GraceAlpha Media . . . . . . . . . . . . . . . . . . . . . . 176 

Vydac ® Media 177 

Davisil ® Media 178–181 

Accessories . . . . . . . . . . . . . . . . . . . . . 182–183 

Tubing, Fittings, Pumps, Injectors, and Swtching Valves 

Flash Chromatography . . . . . . . . . . . . 184–187 

Introduction. . . . . . . . . . . . . . . . . . . . . . . . 184 

GraceResolv Flash Cartridges . . . . . . . . . . . 185–187 

Flash Accessories . . . . . . . . . . . . . . . . . . 186–187 

Flash Media . . . . . . . . . . . . . . . . . . . . . . . . 187 

Thin Layer Chromatography (TLC) . . . . . 188–198 

Introduction. . . . . . . . . . . . . . . . . . . . . . . . 188 

TLC Plates . . . . . . . . . . . . . . . . . . . . . . 189–193 

TLC Accessories . . . . . . . . . . . . . . . . . . . 194–198

prep | flash | tlc 

prep | columns 

Preparative Chromatography Introduction 

Grace ® Key Column Families 

Scalable Solutions: Silica and Hardware 

Grace, the foremost name in silica material science, 

manufactures a range of completely scaleable preparative 

chromatography columns. To help select the appropriate 

column for your application, we describe key column families 

and highlight unique phases within these families. Each 

column family highlighted is also scalable to bulk media and 

available in ton quantities for process scale applications. 

Grace’s silica based media is packed in proprietary column 

hardware that extends media lifetime and ensures high 

column efficiencies at the preparative scale. An overview 

of preparative column hardware can be found on pages 

150–151. 



GraceAlpha combines increased column efficiencies 

and resolution with high loading capacity. A new silica 

(patent pending) makes this possible by combining a 

high-porosity surface, with a dense core, increasing 

mass transfer while yielding a mechanically robust 

particle. 

Column Packing and Operation 

It is generally accepted that the most reliable packing method 

for high-performance preparative columns involve axial 

compression of the adsorbent slurry in the column by a sliding 

piston driven by hydraulic or pneumatic force. However, 

reliable performance and maximum column life are obtained 

only if axial compression force is maintained continuously on 

the column bed during use. The patented MODcol ® Spring 

Column and MultiPacker ® instrument now make it easy and 

convenient for scientists to reproducibly pack any media in a 

completely portable dynamic axial compression column. 

Adsorbent Particle Sizes 

Because small-diameter narrow-particle-range adsorbents 

generally are expensive, it is more economical to use less 

expensive larger diameter materials in preparative columns. 

This is often possible because the objectives of preparative 

separations differ from those of analytical separations. Speed 

and sensitivity may be less important than product purity in 

preparative chromatography. This allows preparative columns 

to be operated at lower flow rates with gradient profiles altered 

in order to compensate for the less efficient mass transfer of 

larger adsorbent particles. To simplify method development 

and scale-up, Grace provides a range of adsorbent sizes and 

grades with identical bonded-phase chemistry. 

Applications: Peptides and small molecules 

(2000 molecular weight) 

Differentiated Phases: C18, C8, and C4 

Specifications: Spheroidal silica, polymerically and 

monomerically bonded, endcapped, 300Å pore size 

Formats: Capillary, LC/MS, Expedite, Rocket, 

Solvent-Reducer, Analytical, Prep, Bulk Media 

Peak Width at Half Height (mm) 

160 

140 

120 

100 

80 

60 

40 

20 

15–20µm 

20–30µm 

5µm 

10µm 

0 

0 200 400 600 800 1000 

Ribonuclease Load (µg) 

7265 

Column Materials: Vydac ® 214TP, 5µm; Vydac ® 214TP, 10µm; 

Vydac ® 214TP, 15 –20µm; 

Vydac ® 214TP, 20 –30µm 

Eluent: 

24 – 95% ACN in 0.1% aqueous TFA over 30min 

at 1.5mL/min 

Protein: 

Ribonuclease 



Preparative Chromatography Introduction 

Preparative Scale Up 

Step 4: The Scale-up Factor 

The column size needed for the purification can be calculated 

based on the output requirements. 

D = diameter L = length 

Loadfinal = Loadinitial x (Dfinal)2 Lfinal 

(Dinitial) 2 Linitial 

The sample load scale-up equation determines the loading 

capacity of the larger preparative column from the loading 

capacity of the analytical column. 

Analytical 

(initial) 

Step 1: Method Optimization 

Prep 

(final) 

The analytical method is optimized to increase the alpha and 

achieve maximum loadability. The most common ways to 

optimize the method are through altering selectivity by mobile 

phase manipulation or by altering the stationary phase. 

Step 2: Loading Study 

Once the analytical method is developed the method 

loadability should be tested to determine the capacity of the 

stationary phase. The sample load will be determined by the 

complexity of the sample mixture as well as the stationary 

phase being used. 

Step 3: Mass Determination 

The total mass that is needed from the purification should 

be determined prior to starting the campaign. The total mass 

needed can be used to balance the necessary throughput, 

purity, and yield required from the separation. 

Step 5: Linear Velocity 

When the column dimensions are changed to optimize 

a separation, or to scale a separation to a preparative or 

narrow-bore application, the mobile phase flow rate should be 

adjusted proportionally to cross-sectional area of the column 

to maintain consistent linear velocity and retention times. See 

table below for common flow rate conversions (Table 1). 

Flow Ratefinal = Flow Rateinitial x (Dfinal)2 

(Dinitial) 2 

Step 6: Run Time and Gradient 

Run Time (prep) = Run Time (analytical) x L (analytical)/L (prep) 

The run time determined by the equation can be used to 

predict the elution time for the compound of interest. To 

retain the resolution obtained on an analytical column while 

increasing column diameter, the gradient shape must be 

maintained by keeping the ratio of the gradient volume to 

the column volume constant. 


Table 1: Common Linear Scale-up Velocity Equivalencies 

Equivalent Linear Velocity Table Relative to 4.6mm i.d. Flow Rates 

Column Diameter (mm i.d.) 1.0 2.1 4.6 10.0 21.5 25 50 100 

Equivalent Flows (mL/min) 0.047 0.208 1.0 4.7 21.8 29.5 118 473 

Equivalent Flows (mL/min) 0.095 0.416 2.0 9.4 43.6 59.0 236 946 

Equivalent Flows (mL/min) 0.142 0.625 3.0 14.1 65.4 88.5 354 1,419 


For analytical HPLC columns, 



For flash cartridges, 


7110 

7278 


149


Preparative Column Hardware 

Grace offers a variety of column formats to provide the best solution at each stage of scale-up. 

All of our columns employ radial flow slots to distribute sample and mobile phase evenly over the 

head of the column for sharp, narrow peaks. Each column is constructed with 316SS, is rated to 

2000psig and uses inert PTFE o-rings and seals. 

Unique radial flow slot design. 

Preparative Column Hardware 

Column Name Available Inner Diameters Attributes 

Prep Guard 7mm • Direct connect to Alltech ® 

Column 

brand columns 


Prep Guard 

Cartridge 

new 

10, 20, 30mm • Holder and inexpensive 

replaceable cartridge 

protects and extends 

column lifetime 

Threaded Column 7, 10, 22mm • Packed under 

high pressure for 

high efficiency 

• Inexpensive 

and disposable 

Flanged Column 25, 30, 50, 100mm • Good flow distribution 

for excellent peak 

shape 

• Robust material of 

construction to enable 

repacking 

Spring Column 25, 50, 100mm • Dynamic axial 

compression for 

extended column 

lifetime 

• High efficiency 

best 

seller 

axial packing 

• Easily repacked 

Axial SFC Column 25, 50mm • Rated to 5000psig 

for use with SFC 

• High efficiency 

axial packing 

• Easily repacked 

tech tip 

30mm i.d. guards can be used with column i.d.s up to 50mm 

when packed with a larger particle size than the column 

being protected. 


Large process scale columns (DAC and 

flanged) up to 1 meter i.d. are available. 

See Peak Biotech columns on page 171 

for more information. 



Spring Column Overview 

Dynamic Axial Compression Column 

• Continuous axial compression prevents void formation, 

assures long column life 

• Use anywhere, just like a standard HPLC column 

• Packed under axial compression for high efficiency 

best 

seller 

The World’s Most Innovative Preparative Column 

Inside every Spring Column unit there is a mechanical 

spring that stores energy, maintaining axial compression 

force on the packed bed. This patented technology is the 

only mobile dynamic axial compression column available 

that prevents void formation. (U.S. Patents No. 5893971, 

5951873, 6036855). 

Spring Columns are available pre-packed with most Grace ® 

adsorbents. Spring Column hardware assures the longest 

column life and the best performance for your prepacked 

preparative column needs. 

Grace ® Multipacker ® packing stations together with empty 

Spring Column hardware provide the option of packing 

your own Spring Column units in house, with any 

high-performance preparative adsorbent of your choice. 

Multipacker ® stations are surprisingly affordable when 

compared to other axial compression packing systems and 

have the advantage of not requiring a dedicated packing 

station for each preparative HPLC location. 


Experience the cost savings and convenience of packing 

your own Spring Columns with the Multipacker ® instrument. 


more info 

For Spring Column ordering information, see pages 162–165. 


Spring column Packing 

Step 1 

Slurry in column. 

Piston and compression 

mechanism in place. 

spring 

Step 2 

Apply axial compression 

force. Force compresses 

spring and moves piston 

to pack adsorbent bed. 

Step 3 

Lock compression 

mechanism. Remove 

external force. Spring 

remains compressed, 

maintains axial force on 

piston and packed bed. 

lock 


151




new 

• High-porosity surface increases mass transfer and results 

in increased column efficiencies and loading capacity 

• Dense core and highly spherical shape yield 

mechanically robust particle 

• Proprietary bonding technology gives unique selectivity 

and increased resolution 

GraceAlpha Performance 

Outstanding Loading Capacity 

GraceAlpha silica has a high-porosity exterior yielding 

higher loading capacities than conventional silicas. 

Unique Selectivity 

Proprietary bonding chemistry provides unique selectivity that 

yields advantages not seen in other C18 and C8 phases. 


Norm. 

800 

600 

400 

200 

Insulin Frontal Loading Capacity 

on C8 Columns with 0.1% TFA 

Insulin loading capacity is 16 to 1000% higher recovery on 

the GraceAlpha C8 column. 

Competitor A 

14 mg/mL 

Competitor B 

133 mg/mL GraceAlpha 

154 mg/mL 

Peptide Standard Mixture 

The GraceAlpha column offers selectivity advantages. 

1 

1 

2 

2 

3 4 

5 

3 4 

5 


Column 

Competitor 

1. GY (238 Da) 

2. VYV (379 Da) 

3. Met Enkephalin 

(YGGFM, 573 Da) 


(DRVYIHPF, 1045 Da) 

5. Leu Enkephalin 

(YGGFL, 555 Da) 

0 

0 20 40 60 80 100 120 140 160 180 

Breakthrough Time (min.) 

High Resolution and Recovery 

GraceAlpha columns increase purity and yield of synthetic 

peptides with superior resolution of impurities due to higher 

mass transfer and optimized C18 and C8 bonding chemistry. 

Crude 20-AA Synthetic Peptide 

The target peptide is better resolved from impurities, with 

33% higher recovery, on the GraceAlpha column. 

0 6 12 18 24 30 Min. 

Columns: All C18, 5µm, 4.6 x 250mm 

Mobile Phase: A: 0.1% TFA in Water, B: 0.085% TFA in Acetonitrile 


%B: 10 40 90 90 



Column Temp.: 25°C 

Resolution (USP)* 

Peptide Peak GraceAlpha Column Competitor 

1 42.0 35.7 

2 32.2 28.3 

3 6.0 2.3 

4 7.4 11.3 

*Resolution is based on the next adjacent peak. 

Better resolution 

of closely eluting 

impurities 

0.00 5.00 10.00 15.00 19.99 

Competitor 

Column 4µm 


Column 5µm 

Columns: All C18, 4.6 x 250mm 

Mobile Phase: A: 0.1% TFA in Water, B: 0.1% TFA in Acetonitrile 


%B: 20 40 


Data courtesy of: Biopeptide Inc. 


GraceAlpha bulk media is also 

available for scale-up. See page 

176 for more information. 

7250 



GraceAlpha Silica 

GraceAlpha (patent pending) is built on a completely 

new silica platform that incorporates over 80 years of 

silica technology at Grace to produce the ultimate in 

chromatographic performance. 

High-porosity exterior 

increases mass transfer. 

Extreme Porosity 

The base silica particle has an extremely high porosity at 

its surface that increases mass transfer leading to higher 

efficiencies and superior loading capacity. The high porosity 

structure has the increased benefit of adding an elastic quality 

to the silica surface allowing the particles to flex and exhibit 

a spring like effect when packed into a column. This further 

stabilizes the packed bed preventing voids from forming and 

allows the particles to pack tighter, yielding higher efficiencies 

in packed beds. 

Dense inner core provides 

exceptional strength 

Dense Core 

Typically the high porosity structure would result in a weak 

particle prone to breaking, but the GraceAlpha particle 

combines the high porosity outer region with a dense inner 

core that results in a particle that resists breaking and forming 

fines that contribute to high backpressures. 

GraceAlpha Silica Specifications 

Pore Size: 

120Å 

Pore Volume: 

0.95mL/g 

Surface Area: 325m 2 /g 

Chemical Purity: 




new 





Prep—Threaded 10 x 150mm 5141840 






Prep—Spring 25 x 150mm 5142081 


Prep—Flanged 30 x 50mm 5142497 


















































GraceAlpha Columns (continued) 














































20µm phases can be custom ordered packed in Spring columns 

or bulk media. 














































Guard Cartridges and Holder 

Format i.d. x Length Qty. Part No. 

Prep—Guard Holder 10 x 10mm ea 2101342 



C18, 5µm 

Prep—Guard Cartridge 10 x 10mm ea 5142000 

Prep—Guard Cartridge 20 x 10mm 2/pk 3118151 


C18, 10µm 




C18, 15µm 




C8, 5µm 




C8, 10µm 




C8, 15µm 




Silica, 5µm 




Silica, 10µm 




Silica, 15µm 





tech tip 

30mm i.d. guards can be used with column i.d.s up to 50mm 

when packed with a larger particle size than the column 

being protected. 

more info 

A guard holder must be purchased separately from the guard 

cartridge for first-time use. Guard holders can then be reused. 


155


Vydac ® MS 300Å Columns 

High Protein Recovery 

• Unique Selectivity for exceptional resolution of proteins 

and peptides 

• High protein recovery improves yield 

A patented surface chemistry reduces adsorption of proteins 

on the silica surface which means higher recoveries and 

better resolution. 

Improved Resolution and Yield 

Vydac ® MS 300Å Silica Specifications 

Pore Size: 

300Å 

Pore Volume: 

0.5mL/g 


Vydac ® MS Phases 

Phase Particle Sizes Carbon Load Endcapped 

C4 5, 10, 15µm 3% Yes 

C8 5, 10, 15µm 5% Yes 

C18 5, 10, 15µm 8% Yes 

The Vydac ® column provides better separation and recovery for a highly hydrophobic membrane protein (RRV p14) and its fatty 

acid modified (myristolyated) form, a component of a potentially new vaccine delivery system. 


1 

2 

4 

4 

3 

2 

4 

1 3 

10 20 30 40 50 60 65 Min. 

1 

2 

Transmembrane Protein p14 on 300Å C4 Columns 

Vydac ® MS 

C4 Column 

Competitor 1 

C4 Column 

Competitor 2 

C4 Column 




4. Triton ® X (Surfactant) 

Column Peak Area 

Peak 2 Peak 3 

MS C4 182 41 

Competitor 1 171 33 

Competitor 2 124 Not 

Detected 

Columns: Vydac ® MS C4 column 214MS54, Competitor 1 C4, 

Competitor 2 C4 (all 300Å, 4.6 x 250mm packed with 

5µm except 4µm for Competitor 1) 

Flow Rate: 

Eluent: 

1.0mL/min 

A: 0.1% v/v TFA in Water 

B: 0.085% v/v TFA in Acetonitrile 

Gradient: 

Vydac ® , Comp. 1: Time: 0 20 25 45 

%B: 20 60 80 80 

Comp. 2: Time: 0 20 25 45 50 80 

%B: 20 60 80 80 90 90 

Detector: 

UV at 215nm 



Reptilian reovirus RRV p14 sample courtesy of Drs. Roberto J. de Antueno and Roy Duncan, Dalhousie University, Halifax, Nova Scotia. 

Recovery of Proteins: MS vs. Leading Columns 

MS C18 recovery: 

• Up to 41% higher at low sample load 

• Up to 29% higher at high sample load 

% Higher Recovery 

60 

50 

40 

30 

20 

10 

MS vs. Leading C18 

Loading 

Amount 

20µg 

50µg 

100µg 

MS C4 recovery: 

• Up to 57% higher at low sample load 

• Up to 12% higher at high sample load 

% Higher Recovery 

60 

50 

40 

30 

20 

10 

MS vs. Leading C4 

Loading 

Amount 

20µg 

50µg 

100µg 

0 

Insulin 

5.7kD 

Ribonuclease 

13.7kD 

Myoglobin 

16.9kD 

0 

Cytochrome C 

11.6kD 

Column: C18 Columns (300Å, 5µm, 4.6 x 150mm): Vydac ® 238MS vs. leading protein/peptide C18 

C4 Columns (300Å, 5µm, 4.6 x 150mm): Vydac ® 214MS vs. leading protein/peptide C4 

Flow Rate: 1.0mL/min. 

Eluent: 

A: 0.1% v/v TFA in Water, B: 0.085% v/v TFA in Acetonitrile 

Temperature: 25°C 


Gradient: C18 Columns = Time: 0 15 20 C4 Columns = Time: 0 12 15 20 

%B: 20 80 80 %B: 20 60 80 80 

BSA 

66.4kD 

Conalbumin 

75.8kD 






C4, 5µm Analytical 4.6 x 250mm 214MS54 

Prep—Threaded 10 x 150mm 214MS51015 









Prep—Flanged 30 x 150mm 214MS103015 
















































Vydac ® MS 300Å Columns (continued) 


















C4, 5µm 

Prep—Guard Cartridge 10 x 10mm ea 214GCC510 

Prep—Guard Cartridge 20 x 10mm 2/pk 214GCC520 

C4, 10µm–15µm 




C8, 5µm 



C8, 10µm–15µm 




C18, 5µm 



C18, 10µm–15µm 





Analytical columns 

and bulk media also 

available, please refer to 

pages 85–87 and 177, 

respectively. 





7110 



157



300Å RP Protein Purification Pioneer 

• Trusted for over 25 years, Vydac ® TP provides reliable, 

high resolution of proteins and large peptides 

• Available in a wide range of bonding chemistries for 

alternative selectivity 

Referenced in over 9000 patents, Vydac ® TP is the 300Å 

phase to which all others are compared. It is relied upon in 

numerous commercial scale protein purifications and, like 

other Grace ® prep columns, is completely scalable to ton 

quantities. 

Vydac ® TP 300Å Silica 

Pore Size: 

300Å 

Pore Volume: 

0.6mL/g 


Vydac ® TP Phases 

Phase Particle Sizes Carbon Load Endcapped 

C4 5, 10, 10–15, 15–20µm 3% Yes 

C8 5, 10, 10–15, 15–20µm 5% Yes 

C18 5, 10, 10–15, 15–20µm 8% Yes 

Diphenyl 5, 10, 10–15, 15–20µm 4% Yes 


Column Selection for Polypeptides 

The reversed-phase column for a polypeptide separation 

should be selected based on the hydrophobicity of the 

polypeptide being chromatographed and molecular weight 

as a secondary consideration. 

Molecular Weight 

1000 

Increasing hydrophilicity 

10,000 

100,000 

C 18 

= 


C 18 

= 218TP, 238TP, 218MS 

C 8 

= 208TP, 208MS 

C 4 

= 214TP, 214MS 

Increasing hydrophobicity 

Diphenyl = 219TP 

Diphenyl columns (219TP, 219MS) are recommended for the 

separation of: 

• Polypeptides with aromatic side chains 

• Large, hydrophobic proteins 

• Lipid peptides 

• Membrane-spanning peptides 

• Fusion proteins from inclusion bodies 

C4 columns (214TP, 214MS) are recommended for the 


• Polypeptides larger than 4000–5000 MW 

• Very hydrophobic polypeptides of any size 

C8 columns (208TP, 208MS) are recommended for the 


• Polypeptides up to 10,000–20,000 MW 



C18 columns (218TP, 218MS, 238TP) 

are recommended for the separation of: 

• Peptides less than 4000–5000 MW 



Purification of Synthetic Peptide 

128mg of a synthetic peptide, GnRH antagonist, was 

purified to 99.7% purity by combining the optimum 

fractions. 1.2g of synthesis mixture was loaded onto a 5 x 

30cm column packed with Vydac ® C18 (218TP), 15–20µm 

and eluted with a gradient of acetonitrile in water containing 

triethylammonium phosphate. 

Absorbance Units (280nm) 

GnRH 

antagonist 

more info 

For more protein and peptide chromatograms, see our 

applications section. 


Analytical columns and bulk media also available, please refer to 

pages 70–71 and 177, respectively. 





0 

60 Min. 






C4 (214TP), 

5µm 

C4 (214TP), 

10µm 

C4 (214TP), 

10 –15µm 

C4 (214TP), 

15 –20µm 

C8 (208TP), 

5µm 

C8 (208TP), 

10µm 

C8 (208TP), 

10 –15µm 

C8 (208TP), 

15 –20µm 

C18 (218TP), 

5µm 

Analytical 4.6 x 250mm 214TP54 

Prep—Threaded 10 x 50mm 214TP51005 











Prep—Flanged 25 x 250mm 214TP1025 





















































Vydac ® TP Columns (continued) 


C18 (218TP), 

10µm 

C18 (218TP), 

10 –15µm 

C18 (218TP), 

15 –20µm 

C18 (238TP), 

5µm 

C18 (238TP), 

10µm 

C18 (238TP), 

10 –15µm 

C18 (238TP), 

15 –20µm 

Diphenyl 

(219TP), 5µm 

Diphenyl 

(219TP), 10µm 
























































Prep—Guard Col. 25 x 30mm 219TP102503 






159






Diphenyl 

(219TP), 

10 –15µm 

Diphenyl 

(219TP), 

15 –20µm 






















C4 (214TP), 5µm 



C4 (214TP), 10µm 




C4 (214TP), 10 –15µm 




C4 (214TP), 15 –20µm 




C8 (208TP), 5µm 



Guard Cartridges and Holder (continued) 


C8 (208TP), 10µm 




C8 (208TP), 10 –15µm 




C8 (208TP), 15 –20µm 




C18 (218TP), 5µm 



C18 (218TP), 10µm 




C18 (218TP), 10 –15µm 




C18 (218TP), 15 –20µm 




C18 (238TP), 5µm 


C18 (238TP), 10µm 


C18 (238TP), 10 –15µm 


C18 (238TP), 15 –20µm 


Diphenyl (219TP), 5µm 


Diphenyl (219TP), 10µm 


Diphenyl (219TP), 10 –15µm 


Diphenyl (219TP), 15 –20µm 


160 

Purification of a Lipid Peptide 

A synthetic peptide containing 25 amino acid residues and two 

attached fatty acids was difficult to purify because of its limited 

solubility and tendency to aggregate. The lower retentivity of the 

Vydac ® 219TP54 diphenyl reversed-phase column was useful for 

purification of this peptide after it was found to be retained so strongly 

that it could not be eluted from a C4 reversed phase. 

Column: 

Flow: 

Detector: 

Vydac ® 219TP54 (diphenyl, 5µm, 4.6mm i.d. x 250mm) 

0.75mL/min 

UV at 280nm 

Trial Chromatogram 

Sample: 250µL of 8:1 diluted peptide solution = 71µg 

Eluent: A: 5% n-propanol/0.5% HOAc/94.5% water 

B: 85% n-propanol/0.5% HOAc/14.5% water 

Gradient: 0 to 100% B in 30min 

Preparative Chromatogram 

Sample: 1.5mL of 3:1 diluted peptide solution = 1.14mg (applied 

as three 500µL injections at 1.5min intervals) 

Eluent: A: 29% n-propanol/0.5% HOAc/70.5% water 

B: 61% n-propanol/0.5% HOAc/38.5% water 

Gradient: Hold 100% A for 6min, then 0 to 100% B in 30min 

In each chromatogram the fine trace is a post-run blank. 

Sample courtesy of Dr. Don Diamond (City of Hope National Medical Center, Duarte, 

Calif.). For a complete description of this method development, request Vydac ® 

Application Note #9802. 

0.016 

0.10 

AU 

0 

AU 

0 

0 

71µg 

Trial 

Chromatogram 

0 20 40 

1.14mg 

Preparative 

Chromatogram 

60 Min. 

20 40 60 Min.


Alltech ® Prep Columns 

• Packed with identical media to analytical columns 

• Additional dimensions may be custom ordered 

Econosphere Columns 


C18, 10µm Prep—Threaded 10 x 100mm 28157 






Silica, 10µm Prep—Threaded 10 x 100mm 28167 



Macrosphere Columns 


GPC 60Å Prep—Threaded 7.5 x 300mm 88175 




Alltima Columns 
































Platinum Columns 


EPS C18, 5µm Prep—Threaded 10 x 150mm 82083 



Prosphere HP 


C18-AQ, 5µm Prep—Threaded 10 x 100mm 35572 



Please refer to pages 44–49, 54–57, and 96–97 for more 

information on media characteristics and analytical column 

offerings. 

Apollo Columns 














Phenyl, 5µm Prep—Threaded 7 x 150mm 36525 












Prevail Columns 


Carbohydrate ES, 5µm Prep—Threaded 7 x 100mm 35103 







6286 6285 

Connect with Direct-Connect Connect In-Line with Fittings 

Coupler. 

and Tubing. 

Prep-Guard Accessories 

Description 

Part No. 

Direct-Connect Column Coupler 28195 

In-Line Connector 9549 

Alltech ® Prep Guards 


Econosphere Guards 

C18, 10µm Prep—Guard 7 x 33mm 970209 


Silica, 10µm Prep—Guard 7 x 33mm 970210 

Alltima Guards 




Platinum Guard 

EPS C18, 5µm Prep—Guard 7 x 33mm 970216 

Prosphere HP Guard 

C18-AQ, 5µm Prep—Guard 7 x 33mm 35584 

Apollo Guards 



Phenyl, 5µm Prep—Guard 7 x 33mm 96430 


Prevail Guards 

Carbohydrate ES, 5µm Prep—Guard 7 x 33mm 35105 




161


Spring Column Hardware 

For Use with Multipacker ® Packing Instruments 

• High-efficiency axial compression packing mechanism 

• Extend media lifetime 

• Mobile Dynamic Axial Compression (DAC) column 

• Easily packed by end-user with a Multipacker ® instrument 

The Spring Column hardware described in this section 

is designed to be used in conjunction with Multipacker ® 

instruments. Please see page 172 for information on the 

Multipacker ® product line. 


Spring Column units are also available prepacked 

and ready-to-use. However, prepacked Spring Column 

units are not user-serviceable and must be returned to Grace 

for repacking, unless the customer owns a Multipacker ® 

packing system. 

How to Order Spring Column Hardware for Use 

with Multipacker ® Instruments 

Spring Columns can be ordered as a complete kit, as seen 

on page 163, or customized selecting the options that best 

suit your needs. Components for customization can be found 

on page 164. Spring Columns are offered in two lengths, 

40cm and 70cm. A 40cm column body can accommodate up 

to a 15cm length packed bed and the 70cm column body can 

accommodate up to a 30cm length packed bed. 

Each complete Spring Column assembly consists of 

five components. For customized columns, one of each 

component below needs to be ordered: 

1. The Base Spring Column Parts Kit includes: 

a. Column Body 

b. All Seals and O-rings 

c. Locking Mechanism 

d. Guide Tube 

e. Endplate 

f. Assembly Hardware 

g. Packing Spacers (for 70cm length columns)—not 

pictured 

2. A Ready-to-Use Piston Assembly consisting of the piston 

body, piston frit, PTFE sleeve and O-ring. The piston 

assembly can be ordered with a 1µm, 2µm, or 5µm frit 

porosity. 

3. A Spring Kit. Choose the appropriate pressure range 

for your needs. Spring force must exceed the operating 

pressure of the column in order to maintain axial 

compression force. 

4. An Outlet Frit. Choose 1µm, 2µm, or 5µm frit porosity. 

For 101mm i.d. columns, the outlet frit is identical to the 

piston assembly (#2). 

5. Tubing and Connectors. Choose tubing with an outer 

diameter and bore that suits your needs. 

c 

2 


Refer to page 172 for more 

information on the Multipacker ® 

packing stations used to pack 

Spring Column hardware. 

7410 

Base Spring Colum Parts Kit 

4 

b 

3 

d 

a 

e 

f 

The guides on the following pages will help you select the 

combination of options appropriate to your needs. For further 

assistance, call Grace Technical Support. 

7403 


Contact Tech Support: Phone: 1.800.255.8324 






Complete Spring Column Hardware Kits 

Complete hardware kits are available under single catalog numbers for standard 25mm i.d, 50mm i.d, and 101mm i.d. Spring 

Column units, as shown below. 

Complete kits are provided with 2µm frits and 1000 to 2000psig springs. Different combinations are possible by building a custom 

Spring Column on page 164. 

Individual components and replacement parts are listed on the following two pages. 

Complete Spring Column Hardware Kits 

Column Dimensions Part No. Description 

25mm i.d. 

25mm i.d. x 40cm, 

Non-Water-Jacketed 

25mm i.d. x 70cm, 

Non-Water-Jacketed 

50mm i.d. 

50mm i.d. x 40cm, 

Non-Water-Jacketed** 

50mm i.d. x 70cm, 

Non-Water-Jacketed** 

101mm i.d. 

101mm i.d. x 40cm, 

Water-Jacketed 

101mm i.d. x 70cm, 


SA2002-025040 

AS2002-025A09 

AF0000-C02502 

AS2002-025A03 

AS2002-025A07 

SA2002-025070 

AS2002-025A09 

AF0000-C02502 

AS2002-025A03 

AS2002-025A07 

SA2002-050040 

AS2002-050A01 

AF0000-B05002 

AS2002-050A03 

AS2002-050A07 

AS2002-050A09 

SA2002-050070 

AS2002-050A01 

AF0000-B05002 

AS2002-050A03 

AS2002-050A07 

AS2002-050A09 

SA4004-101040 

2 x AS4004-101A06 

AS4004-101A03 

AS2002-050A08 

SA4004-101070 

2 x AS4004-101A06 

AS4004-101A03 

AS2002-050A08s 

Includes: 

Base Column Parts Kit*, 40cm long column, for bed lengths up to 15cm 

Piston Assembly with 2µm frit 

Outlet Frit, 2µm 

Spring Kit for packing pressures from 1000 to 2000psig 

1/16” o.d., 0.040” i.d. Connecting Tube and Fittings 




















Spring Kit for packing pressures up to 2000psig 






Part No. 

SC2002-025040 

SC2002-025070 

SC2002-050040 

SC2002-050070 

SC4004-101040 

SC4004-101070 

NOTE: 

Spring Column units are designed to be easily serviceable. A full array of components and replacement parts are available. Please consult the listings on the following two pages as 

well as the column manuals for details. 

*See page 162 for listing of components included in Base Column Parts Kit. 

**Two sizes of connecting tubing provided. 


7412 


163



Spring Column Components 

Experienced Spring Column users can build customized Spring Column units by individually selecting the components that 

best meet their needs. A total of five components is needed to assemble a complete Spring Column. 


Spring Column Units 

Column Dimensions Description Part No. 

25mm i.d. 

1. Base Parts Kit Non-Water-Jacketed Kit, 40cm long, for bed lengths up to 15cm SA2002-025040 

Water-Jacketed Kit, 40cm long, for bed lengths up to 15cm 

SA2002-W25040 

Non-Water-Jacketed Kit, 70cm long, for bed lengths up to 30cm 

SA2002-025070 


SA2002-W25070 

2. Piston Assembly Piston Assembly with 1µm frit AS2002-025A01 


AS2002-025A09 


AS2002-025A02 

3. Spring Kit Spring Kit for packing pressures from 1000 to 2000psig AS2002-025A03 


AS2002-025A04 


AS2002-025A05 

4. Outlet Frit Outlet Frit, 1µm AF0000-C02501 


AF0000-C02502 


AF0000-C02503 

5. Tubing and Connectors 1/16” o.d., 0.020” i.d. Connecting Tube and Fittings AS2002-025A06 

1/16” o.d., 0.040” i.d. Connecting Tube and Fittings AS2002-025A07 

50mm i.d. 

1. Base Parts Kit Non-Water-Jacketed Kit, 40cm long, for bed lengths up to 15cm SA2002-050040 


SA2002-W50040 


SA2002-050070 


SA2002-W50070 

2. Piston Assembly Piston Assembly with 1µm frit AS2002-050A01 


AS2002-050A11 


AS2002-050A02 



AS2002-050A05 


AS2002-050A06 

4. Outlet Frit Outlet Frit, 1µm AF0000-B05001 


AF0000-B05002 


AF0000-B05003 




101mm i.d. 

1. Base Parts Kit Non-Water-Jacketed Kit, 40cm long, for bed lengths up to 15cm SA4004-N10140 


SA4004-101040 


SA4004-N10170 


SA4004-101070 

2. Piston Assembly 


AS4004-101A01 

(Need Qty. 2) 


AS4004-101A06 


AS4004-101A02 



AS4004-101A04 


AS4004-101A05 



more info 

For a listing of the individual parts included in the kits and 

assemblies above, please refer to page 162. 

tech tip 

Selecting the correct frit porosity ensures particles are retained in 

the column while contributing minimally to backpressure. See the 

recommendation below. 

Particle Size 

Recommended Frit Porosity 

10µm 5µm 




Repack Kits for Spring Columns 

Popular frit porosities, 2µm and 5µm, are available bundled with the appropriate replacement consumables needed when 

repacking. 1µm frits are available below in the replacement parts list. 

The 25 and 50mm i.d. repack kits contain consumable parts for three repacks. The 101mm i.d. repack kits contain parts for 

one repack. 

Repack Kits for Spring Column Modules 

Includes: 

Column Dimensions Qty. Part No. Description 

25mm i.d. 

With 2µm Frits 3 

3 

3 

3 

AF0000-C02502 

AH0000-R025A1 

AF0000-D02501 

AS2002-025A14 


Outlet O-ring Set 

Outlet Disperser Frit 

Piston Frit Replacement Kit*, 2µm 


3 

3 

3 

50mm i.d. 


3 

3 

3 


3 

3 

3 

101mm i.d. 


2 

2 


2 

2 

AF0000-C02503 

AH0000-R025A1 

AF0000-D02501 

AS2002-025A13 

AF0000-B05002 

AH0000-R050A1 

AF0000-D05001 

AS2002-050A16 

AF0000-B05003 

AH0000-R050A1 

AF0000-D05001 

AS2002-050A15 

AS4004-101A18 

AS4004-101011 

AS4004-101012 

AS4004-101A17 

AS4004-101011 

AS4004-101012 

*Components of the Piston Frit Replacement Kit are listed below. 














Piston O-ring 

PTFE Sleeve 


Piston O-ring 

PTFE Sleeve 

Part No. 

AS2002-025A16 

AS2002-025A17 

AS2002-050A18 

AS2002-050A19 

AS4004-101A20 

AS4004-101A21 


Replacement Parts for Spring Columns 

In addition to the repack kits listed above, a full array of individual component replacement parts may be ordered from the list below. 

Spring Column Replacement Parts 

Description For 25mm i.d. For 50mm i.d. For 101mm i.d. 

Top Restraint — — AS4004-101008 

Bottom Restraint — — AS4004-101009 

Piston Top — — AS4004-101010 

Piston O-ring AS2002-025011 AS2002-050011 AS4004-101011 

Guide Tube AS2002-025013 AS2002-050013 AS4004-101013 

Column Body, 40 cm Long Non-Water-Jacketed FS2002-025040 FS2002-050040 AS4004-N10140 

Column Body, 70 cm Long Non-Water-Jacketed FS2002-025070 FS2002-050070 AS4004-N10170 

Column Body, 40 cm Long Water-Jacketed FS2002-W25040 FS2002-W50040 AS4004-101040 

Column Body, 70 cm Long Water-Jacketed FS2002-W25070 FS2002-W50070 AS4004-101070 

Outlet Disperser Frit AF0000-D02501 AF0000-D05001 — 

Outlet Frit, 1µm AF0000-C02501 AF0000-B05001 — 



Outlet O-ring Set AH0000-R025A1 AH0000-R050A1 — 

Replacement Bolts, Nuts, and Washers for Non-Water-Jacketed Column AS2002-025A08 AS2002-050A10 — 

Replacement Bolts, Nuts, and Washers for Water-Jacketed Column AS2002-025A11 AS2002-050A13 — 

Threaded Spacer for 40cm Long Column AS2002-025000 AS2002-050000 AS4004-101000 

Threaded Spacer for 70cm Long Column AS2002-025001 AS2002-050001 AS4004-101001 

Threaded Flange AS2002-025002 AS2002-050002 AS4004-101002 

Packing Spacer AS2002-025003 AS2002-050003 AS4004-101003 

Unpacking Spacer for 40cm Long Columns AS2002-025004 AS2002-050004 AS4004-101004 

Unpacking Spacer for 70cm Long Columns AS2002-025005 AS2002-050005 AS4004-101005 

Piston AS2002-025007 AS2002-050007 AS4004-101007 

Piston Frit Replacement Kit (Piston O-ring, 1µm Double Density Frit, PTFE Sleeve), 1µm AS2002-025A12 AS2002-050A14 AS4004-101A16 



PTFE Sleeve AS2002-025010 AS2002-050010 AS4004-101012 


165


Axial SFC Column Hardware 

For Use with Multipacker ® Packing 

Instruments 

Rugged SFC Sealing System 

The sealing materials for axial SFC columns have been 

chosen for their compatibility with modified supercritical 

carbon dioxide typically employed in SFC systems. The seal 

system is rated for continuous operation at pressures of up 

to 5000psig (345 bar). 

Piston 

A pure PTFE O-ring primary seal and a Turcon ® spring-loaded 

lip secondary seal comprise the sealing mechanism for the 

piston. 


• High efficiency packing mechanism 

• Rated to 5000psig for SFC 

• Easily packed by end-user with Multipacker ® instrument 

Grace Axial SFC Columns are design-related to Spring 

Column axial compression columns. They are packed with 

the same, easy-to-use Multipacker ® packing systems but 

are designed with the requirements of supercritical fluid 

chromatography in mind. Custom seals, suitable for use 

with supercritical CO2 at pressures up to 5000psig, are 

manufactured specially for Grace. The axial compression 

packing mode is still employed. However, the internal spring 

axial compression has been omitted because of the high 

pressures typical in SFC systems and the low visosity of the 

mobile phase, which makes void formation less likely. 

7413 

Column Outlet 

7405 7406 

The column outlet seals system is composed of a Turcon ® 

spring-loaded lip seal and a bronze backup ring. 

Axial SFC Column Hardware Specifications 

Wet Surfaces: Electropolished Type 316 Stainless Steel, PTFE 

Column Body: Electropolished Type 316 Stainless Steel 

Piston: 

Electropolished Type 316 Stainless Steel 

Threaded Spacer: Type 6061 Aluminum 

Endplate: 

Electropolished Type 316 Stainless Steel 

Bolting Hardware: Grade 8, qualified for pressure vessels 

Frits: 

Passivated Sintered Type 316 Stainless Steel 

O-ring Seals: 

PTFE 

Lip Seals: Turcon ® 

Backup Ring: Bronze 

Pressure Rating: 5000psig (345 bar) 

Max. Temperature: 135°C (275°F) 


Refer to page 172 for more 

information on the Multipacker ® 

packing stations used to pack 

Axial SFC column hardware. 

7407 7408 

7403 



Axial SFC Column Hardware 

Complete Axial SFC Column Hardware Kits 

Complete hardware kits are available under single catalog numbers for standard 25mm i.d. and 50mm i.d. water-jacketed axial 

SFC columns, as shown below. Individual components and replacement parts are listed on the following two pages. 

Axial SFC Columns 


25mm i.d. 

25mm i.d. x 40cm, 


25mm i.d. x 70cm, 


50mm i.d. 

50mm i.d. x 40cm, 


50mm i.d. x 70cm, 


AY2002-W25040 

AY2002-025A01 

AF0000-C02501 

AS2002-025A07 

AY2002-W25070 

AY2002-025A01 

AF0000-C02501 

AS2002-025A07 

AY2002-W50040 

AY2002-050A01 

AF0000-B05001 

AS2002-050A07 

AY2002-W50070 

AY2002-050A01 

AF0000-B05001 

AS2002-050A07 

*See page 162 for listing of components included in Base Column Parts Kit. 

Includes: 

















Part No. 

SY2002-W25040 

SY2002-W25070 

SY2002-W50040 

SY2002-W50070 

more info 

Supercritical Fluid Chromatography (SFC) 

Supercritical fluids, first discovered in 1879, have been used for extraction applications since the 1950s. The 1980s saw an increase in their 

use as mobile phases for supercritical fluid chromatograpy (SFC). The mobile phase most commonly used in SFC is supercritical CO2. 

Supercritical CO2 as a mobile phase provides high solubility and high column loading for many substances. In addition, CO2 and other 

supercritical fluids have very low viscosity which enables faster separations and extractions. Collection of concentrated product is also 

facilitated by the ready evaporation of the mobile phase under ambient conditions. 

SFC today is just another type of high-performance chromatography, either normal or reversed phase, ideally suited for the analysis of 

thermally labile molecules with low or moderate molecular weight. 

Any molecule that will dissolve in methanol or a less polar solvent is a good candidate for SFC. Many strong bases that are difficult to 

separate by other techniques separate rapidly and efficiently with good peak shape in SFC. 

This technique in recent times has attracted new applications in drug discovery and drug purification because of its speed of separation 

and simplicity of fraction collection. 

SFC requires columns with hardware and seals designed to withstand higher operating pressures. Grace’s Axial SFC columns are 

designed to meet these requirements and offer in addition the advantage of a long-life column prepared using proven axial compression 

packing techniques. 







167


Axial SFC Column Components 

How to Order Axial SFC Columns 

Axial SFC columns can be ordered as a complete kit, as seen 

on page 167, or customized selecting the options that best suit 

your needs, as shown below. Axial SFC columns are offered 

in two lengths, 40cm and 70cm. A 40cm column body can 

accommodate up to a 15cm length packed bed and the 70cm 

column body can accommodate up to a 30cm length packed 

bed. 


Each complete column consists of four components: 

1. The Base Spring Column Parts Kit includes: 

a. Column Body Axial SFC 

b. All Seals and O-rings 

c. Locking Mechanism 

d. Guide Tube 

e. Endplate 

f. Assembly Hardware 

g. Packing Spacers (for 70cm length columns) 

2. A Ready-to-Use Piston Assembly consisting of the piston 

body, piston frit, PTFE sleeve, and piston seal. The piston 

assembly can be ordered with a 1µm or 5µm frit porosity. 

3. An Outlet Frit. Choose 1µm or 5µm frit porosity. 

4. Tubing and Connectors. Choose tubing with an outer 

diameter and bore that suits your needs. 

7413 

Experienced Axial SFC Column users can build customized Axial SFC Columns by individually selecting the components that 

best meet their needs. 

Axial SFC Columns 

Column Dimensions Description Part No. 

25mm i.d. 

1. Base Parts Kit Non-Water-Jacketed Axial SFC Column Kit, 40cm long, for bed lengths up to 15cm AY2002-025040 

Water-Jacketed Axial SFC Column Kit, 40cm long, for bed lengths up to 15cm 

AY2002-W25040 

Non-Water-Jacketed Axial SFC Column Kit, 70cm long, for bed lengths up to 30cm 

AY2002-025070 


AY2002-W25070 

2. Piston Assembly Piston Assembly with 1µm frit AY2002-025A01 


AY2002-025A02 

3. Outlet Frit Outlet Frit, 1µm AF0000-C02501 


AF0000-C02503 

4. Tubing and Connectors 1/16” o.d., 0.020” i.d. Connecting Tube and Fittings AF2002-025A06 

1/16” o.d., 0.040” i.d. Connecting Tube and Fittings AF2002-025A07 

50mm i.d. 

1. Base Parts Kit Non-Water-Jacketed Axial SFC Column Kit, 40cm long, for bed lengths up to 15cm AY2002-050040 


AY2002-W50040 

Non-Water-Jacketed Axial SFC Column Kit, 70cm long, for bed lengths up to 30cm 

AY2002-050070 


AY2002-W50070 

2. Piston Assembly Piston Assembly with 1µm frit AY2002-050A01 


AY2002-050A02 

3. Outlet Frit Outlet Frit, 1µm AF0000-B05001 


AF0000-B05003 

4. Tubing and Connectors 1/16” o.d., 0.040” i.d. Connecting Tube and Fittings AF2002-050A07 



more info 

For a listing of the individual parts included in the kits and assemblies above, please refer to page 166. 



Axial SFC Column Components 

Repack Kits for Axial SFC Columns 

Each repack kit contains consumable parts for one column repack. 

Repack Kit for Spring Columns 


25mm i.d. 

With 1µm Frits 


50mm i.d. 



AF0000-C02501 

AH0000-R025A2 

AF0000-D02501 

AY2002-025A12 

AF0000-C02503 

AF0000-D02501 

AH0000-R025A2 

AY2002-025A13 

AF0000-B05001 

AH0000-R050A2 

AF0000-D05001 

AY2002-050A14 

AF0000-B05003 

AF0000-D05001 

AH0000-R050A2 

AY2002-050A15 

*Components of the Piston Frit Replacement Kit are listed below. 

Replacement Parts for Axial SFC Columns 

Includes: 


Seal Ring Set for Axial SFC Column Outlet 















In addition to the repack kits listed above, a full array of replacement parts may be ordered from the list below. 

Part No. 

AY2002-025A14 

AY2002-025A15 

AY2002-050A12 

AY2002-025A15 


Spring Column Replacement Parts 

Description For 25mm i.d. For 50mm i.d. 

Outlet Disperser Frit AF0000-D02501 AF0000-D05001 

Outlet Frit, 1µm AF0000-C02501 AF0000-B05001 

Outlet Frit, 5µm AF0000-C02503 AF0000-B05003 

Seal Ring Set for Axial SFC Column Outlet AH0000-R025A2 AH0000-R050A2 

Replacement Bolts, Nuts, and Washers for Non-Water-Jacketed Column AY2002-025A08 AY2002-050A10 

Replacement Botls, Nuts, and Washers for Water-Jacketed Column AY2002-025A11 AY2002-050A13 

Threaded Spacer for 40cm Long Column AS2002-025000 AS2002-050000 

Threaded Spacer for 70cm Long Column AS2002-025001 AS2002-050001 

Threaded Flange AS2002-025002 AS2002-050002 

Packing Spacer AS2002-025003 AS2002-050003 

Unpacking Spacer for 70cm Long Columns AS2002-025005 AS2002-050005 

Piston AY2002-025007 AY2002-050007 

Piston Frit Replacement Kit, 1µm; Piston O-ring, spring-loaded seal, PTFE sleeve, 1µm Double-Density Frit AY2002-025A12 AY2002-050A14 

Piston Frit Replacement Kit, 5µm; Piston O-ring, spring-loaded seal, PTFE sleeve, 5µm Double-Density Frit AY2002-025A13 AY2002-050A15 

Piston Seal for Axial SFC Column AY2002-025011 AY2002-050011 

PTFE Sleeve AS2002-025010 AS2002-050010 


169


Empty MODcol ® Flanged Columns 

• Robust 316 stainless steel construction 

• Double seals prevent leaks 

• Novel flow distribution ensures high chromotographic performance 

Modular in construction and manufactured to the highest standards, these patented stainless steel columns are known for 

durability and their special leakproof seal system. A uniform approach to the design of MODcol ® column components is used 

across the MODcol ® preparative, semi-preparative and industrial column product lines. 


All MODcol ® flanged columns are constructed from high-quality 316 stainless steel alloy and electro-polished to provide the 

highest possible corrosion protection and chemical inertness. The flanged construction is rugged and conservatively rated at 

2000psig. Column closure is achieved with high-strength bolts and heavy-duty endplates that are engineered to provide long 

product life. The simplicity of this design, without threads or other parts that can wear out, makes the hardware reusable and 

repackable. 

Each empty column kit comes with the following: 

• One column body 

• One pair of end plates 

• Frits, o-rings, and tubing connectors 


Custom packing of MODcol ® Flanged Columns is available. 

Refer to page 174. 

Sizes other than those listed are available on a custom basis. Please contact Grace for assistance. 

7347 

7372 

Empty MODcol ® Flanged Column Kits (Complete) 

i.d. 

Length 

5cm Part No. 10cm Part No. 15cm Part No. 25cm Part No. 30cm Part No. 50cm Part No. 

25mm FA0000-025005 FA0000-025010 FA0000-025015 FA0000-025025 C C 

30mm FA0000-030005 FA0000-030010 FA0000-030015 FA0000-030025 C C 

50mm FA0000-050005 FA0000-050010 FA0000-050015 FA0000-050025 FA0000-050030 FA0000-050050 

101mm — FA0000-101010 FA0000-101015 FA0000-101025 FA0000-101030 FA0000-101050 

C = available as a custom order. 

Parts for MODcol ® Flanged Columns 

Assembly Hardware 

Seal Rings/O-Rings (set) 

Disperser Frits (pair) 

Bed-Support Frits (pair)* 

i.d. 

Part No. 

Part No. 

Part No. 

Part No. 

25mm AH0000-A02501 AH0000-R025A1 AF0000-D025A1 AF0000-B025Ax 




*When specifying the part number for flanged column frits, please indicate the desired porosity by substituting the trailing “x” with: “1” for 1µm, “2” for 2µm, “3” for 5µm, “4” for 10µm. 

MODcol ® Mobile Column Stand, Holder, and Tray 

i.d. 

Part No. 

101mm A00000-101000 

Stainless Steel Tubing, Fittings, and Connectors 

Description 

Part No. 

1/16” o.d., 0.020” i.d. Connecting Tube and Fittings* AS2002-025A06 


1/8” o.d., 0.060” i.d. Connecting Tube and Fittings** AS2002-050A08 


*1 union, 1 nut, 1 ferrule, and 2 plugs. Compatible with flanged and spring columns up to 25mm i.d. 

**1 union, 1 nut, 1 ferrule, 2 plugs, and 2 female reducers 1/8” to 1/16.” Compatible with flanged and spring columns of 30mm i.d. and greater. 



Process Scale Columns and Systems by Peak Biotech A/S 

Grace is a global distributor of Peak Biotech A/S columns and systems. This partnership with Peak extends Grace’s column line 

to provide customers a completely scalable solution, from pre-packed prep columns to bulk chromatography adsorbents and 

process scale columns and systems. The coordination between bulk media and process column manufacturer reduces scale-up 

risk. Peak’s products are of the highest quality, and are trusted throughout the pharmaceutical and biotech industry. 

Dynamic Axial Compression Columns: 50–1000mm i.d. 

Features: 

• Easy packing and unpacking 

• Uniform distribution system 

• Removable frit design for easy cleaning 

or replacement 

• High performance and reproducibility 

• Leak detection system 

• No sealing adjustment needed 

• Scalable 

• Mobile construction up to 300mm 

• Ergonomic working positions 

High-Pressure Chromatography Systems 

Features: 

• Gradient performance with high 

accuracy 

• Fully automated, CFR Part 11 and 

GAMP 4 compliant 

• Mobile construction 

• Complete unit, tested and documented 

• Compact and reliable 

• Flexible control 

• Easy maintenance 

• Sanitary design 

Column Specifications: 

Diameters: 

From 50–1000mm 

Pressure Rated: To 100 bar 

Sealing Materials: PTFE and PEEK 

Material of Construction: 1.4404, 1.4435, SS316L, 

SAF2205 

Construction Code: PED or ASME 

System Specifications: 

Flow Rates: 

Flow Rates: 

Pressure Rating: 

Gradient Loop: 

Inlets: 

Sealing Material: 

Material of 

Construction: 

6–5000L/h for medium pressure 

6–1000L/h for high pressure 

0–20 bar and 0–80 bar 

Feedback for NIR, mass or 

conductivity 

4 buffer inlets, 1 product and 

5 fractions 

PTFE 

1.4404, 1.4435, SS316L 


Software Control 

Features: 

• Based on Siemens PCS 7 

• Fully automated 

• CFR Part 11 compliant 

• GAMP 4 compliant 

• Programmed according to S88 standard 

• Fully tested and validated 

• Flexible control 

ordering information 

Contact your Grace sales representative or customer service 

to review the column and system options and custom build your 

Peak Biotech system. 


Also available from Peak Biotech: 

• Slurry Handling Equipment 

• Ultra-sonic Baths 

• Buffer Systems 

• Tanks 

• Flanged Columns / mech. compress columns 

• Low and medium pressure columns 

• Low and medium pressure systems 


171


Multipacker ® Packing Stations 

• Axial packing for high efficiency 

• Identicle, high performing columns every time 

• Easy to use 

• Pack multiple columns on demand 

• Economically priced 

Multipacker ® instruments are easy-to-use high-performance 

packing stations specifically designed for packing Spring 

Columns. Three separate instruments are available for 

packing 25mm i.d., 50mm i.d., and 101mm i.d. columns. 

Their ease of operation and short cycle times provide a 

self-contained versatile column-packing solution. 

prep | column packing 

Spring Column hardware offers outstanding convenience 

and flexibility when used with Multipacker ® instruments. 

The ability to vary bed length continuously up to 30cm and 

the advantages of the Spring-driven axial compression 

mechanism provide a high-performance adaptive solution 

for both product development and process chromatography. 

Furthermore, compared to traditional column packing 

processes, Spring Column packing requires significantly 

less adsorbent per column because the bed is formed from 

the entire quantity of adsorbent introduced into the column, 

without wastage. More columns can be packed from the 

same batch of medium, reducing concerns about lot-tolot 

variations. Also, because adsorbent requirements are 

reduced, the cost of a Multipacker ® instrument is recovered 

quickly. 

Once packed, Spring Column units are independent and 

maintain axial compression when used separately from the 

Multipacker ® instrument. Consequently, one Multipacker ® 

instrument can be used to maintain an entire fleet of Spring 

Column modules. For the first time, chromatographers have 

the ability to pack columns on demand and quickly respond to 

in-house column needs. 

25 and 50mm i.d. Multipacker ® Instruments. 

7403 

Multipacker ® instruments provide several unique advantages. 

You have the ability to pack columns when and where they 

are needed. You save money with every column packed and 

less adsorbent is required. You have control of bed length and 

column volume, and columns can easily be unpacked, with 

recovery of adsorbent, and repacked in a matter of minutes. 

A Multipacker ® axial-compression system is able to pack 

columns at low pressures, so less robust media can be 

protected from damage and their performance preserved. 

Reduced mechanical damage to adsorbent with a 

Multipacker ® instrument makes it the perfect match for 

delicate, non-silica-based media. 

Unlike column-packing systems that use awkward hand 

pumps and can require hours for column packing, Spring 

Column packing takes only minutes and operates with the 

push of a button. Multipacker ® instruments use modular 

construction that makes them lightweight and easily 

assembled or disassembled. Key components are produced 

using advanced aluminum casting techniques that reduce 

manufacturing costs and provide high strength and reliability. 






Refer to pages 162–169 for more information on Spring 

Columns for use with Multipacker ® packing stations. 

7412 



Multipacker ® Packing Stations 

The World’s Most Innovative Preparative Column Packing System 

Multipacker ® Series 2002 Instrument for 25mm i.d. Spring Column Modules 

Part No. 

IM2002-025IM1 

Multipacker ® Series 2002 Instrument for 50mm i.d. Spring Column Modules 

Part No. 

IM2002-050IM1 

Multipacker ® Series 2002 Two-Instrument Bundle for 25mm i.d. and 50mm i.d. Spring Column Modules 

Part No. 

IM2002-025050 

Multipacker ® Instrument for 101mm i.d. Spring Column Modules 

Part No. 

IM2002-101IM1 

more info 

For a short web animation and video demonstration, 

visit the MODcol ® section of our website at 

www.discoverysciences.com/modcol. 



Multipacker ® instruments require Spring Column hardware, 

purchased separately, for column packing. See listing on 

pages 162–169. 

101mm 

Multipacker ® 

Instrument. 

7404 


173


Column Packing Services 

• Experience packing hundreds of types of media, Grace 

and non-Grace media 

• Packing service available for Spring Columns and 

Flanged Column hardware 

• Column Efficiency Report (CER) provided with each 

column 

Since 1982 Grace’s MODcol ® division has successfully 

packed thousands of preparative columns with hundreds 

of different media. Proven proprietary procedures together 

with quality hardware allow Grace to provide preparative 

columns with high efficiency, excellent resolution and low 

backpressure. 

Column Efficiency Report (CER) 


The result of each packing is validated and presented to the 

customer in the form of a Column Efficiency Report (CER). 

This report summarizes the product, the testing protocol, and 

presents the chromatographic data in a graphical and tabular 

format that is easy to understand. Only when the customer 

approves the result is the column shipped. 

Grace supplies end users with the world’s only ready-to-use 

axial compression system, the prepacked Spring Column 

unit. Empty Spring Column hardware can be ordered and, 

combined with media purchased from Grace or supplied by 

the end user and the packing services shown below, delivered 

as a ready-to-use product. Grace packs Spring Column 

units using the Multipacker ® instruments described earlier in 

this section. Purchasing a prepacked Spring Column unit is 

a great way to evaluate the performance of this novel product 

under real-world conditions. Unpacking and repacking the 

Spring Column unit must be done by Grace or on a user’s 

own Multipacker ® instrument. 

Each custom-packed Spring Column unit is validated as 

described above. 

Please note that the 40cm long Spring Column body can 

accommodate bed lengths up to15cm, and the 70cm-long 

Spring Column body can accommodate a maximum bed 

length of 30cm. For intermediate beds, please specifiy the 

length to be packed by modifying the last two digits of the part 

number shown, e.g., for a 50mm i.d. x 40cm Spring Column 

unit packed to a bed height of 12cm, specify part number 

PS0000-050412. For information concerning the amount of 

media that will be required to pack a particular bed length 

please call your local sales person, distributor, or Grace 

technical support. 

7389 





Column Packing Services for Flanged Columns 

i.d. 

Length 

5cm 10cm 15cm 25cm 30cm 50cm 

25mm PA0000-025005 PA0000-025010 PA0000-025015 PA0000-025025 C C 

30mm PA0000-030005 PA0000-030010 PA0000-030015 PA0000-030025 C C 

50mm PA0000-050005 PA0000-050010 PA0000-050015 PA0000-050025 PA0000-050030 PA0000-050050 

101mm — PA0000-101010 PA0000-101015 PA0000-101025 PA0000-101030 PA0000-101050 

C = available as custom. 

Column Packing Services for Spring Column and Axial SFC Column 

Column i.d. 

Bed Length 

5cm 10cm 15cm 25cm 30cm 

25mm x 40cm PS0000-025405 PS0000-025410 PS0000-025415 — — 

25mm x 70cm PS0000-025705 PS0000-025710 PS0000-025715 PS0000-025725 PS0000-025730 

50mm x 40cm PS0000-050405 PS0000-050410 PS0000-050415 — — 


101mm x 40cm PS0000-101405 PS0000-101410 PS0000-101415 — — 




Bulk Chromatographic Media Introduction 

Grace–The Foremost Name in Silica Materials Science 

Davison Chemical, forerunner of the Grace Davison Silica Products business, was founded in Baltimore, 

Maryland in 1832. Silica gel, was patented in 1919 and in the early 1920s Davison commercialized silica gel 

and began developing many new applications such as air drying, refrigeration, and packaging desiccants. 

Grace Davison has continued to expand applications for silica and has long been recognized for superior 

technical service, strong applications technology, and global customer support. 

Quality, Consistency, and Proven Performance 

Grace Davison’s materials science expertise assures highquality 

and consistency in its silica-based media products. 

Our bulk adsorbents are used to purify a number of FDA 

approved biopharmaceuticals and we provide regulatory 

support data for GMP applications. 

All of our plants are ISO-9001 certified and 

our Hesperia, California plant is compliant with 

the IPEC-PQG GMP guidelines. 

Unmatched Capacity, Global Reach, Expert Support 

Our global presence and manufacturing capacity enables us to supply hundreds of tons of silica every year to some of the largest 

biotechnology and pharmaceutical manufacturing facilities. When you rely on Grace, you can count on consistent product quality, 

timely delivery and expert technical support. 

Grace Key Bulk Media Families 

Scalable Solutions: Silica and Hardware 

Grace manufactures a range of completely scalable silica 

media. To help select the appropriate media for your 

applicaiton, we describe key media families and highlight 

unique phases within these families. Each family highlighted 

is available up to ton quantities for process scale applications. 

Grace also provides a range of columns and skid systems 

from preparative to process scale that ensure optimal 

performance of our media (pages 162–174). Please contact 

your Grace sales representative to discuss a complete 

solution for your preparative or process scale needs. 


A new silica generation. 

GraceAlpha combines increased column efficiencies 

and resolution with high loading capacity. A new silica 

(patent pending) makes this possible by combining a 

high-porosity surface, with a dense core, increasing 

mass transfer while yielding a mechanically robust 

particle. 

Applications: High purity spherical silica suitable for 

peptides and small molecules (2000 molecular weight) 

Differentiated Phases: C18, C8, C4 

Specifications: Spheroidal silica, polymerically and 

monomerically bonded, endcapped, 300Å pore size, 

10μm–30μm 

Formats: Capillary, Microbore, Expedite, Rocket, 

Solvent-Reducer, Analytical, Prep, Bulk Media 

Davisil ® 

Excellent mechanical and structural properties, 

Davisil ® silica are globally recognized for their 

consistency and reliability. 

The Davisil ® silica line offers a range of pore diameters 

from 30Å to 2500Å with high surface area and narrow 

particle size distributions to offer the chromatographer 

the ultimate in selection and performance. 

Applications: Economical granular silica for small 

molecules to large biomolecules 

prep | media 

Differentiated Phases: Silica, C18, Cyano, Diol, Amino 

Specifications: Granular silica, 30–2500Å pore sizes, 

10μm–2000μm particle sizes 

Format: Bulk Media 


175


GraceAlpha Media 


new 

• Increased loading capacity 

• High resolution 

• High efficiency DAC packing 

• Produced in facility complaint to IPEC-PQG GMP 

guidelines 

• Ultimately reproducible 

• Mechanically strong 

GraceAlpha Silica Specifications 

Pore Size: 

120Å 

Pore Volume: 

0.95mL/g 


Chemical Purity: 


Vydac ® Media 

300Å RP Protein Purification Pioneer 

Pioneered by Grace and produced by a unique 

process based on purified oranosilicate starting materials, 

Vydac ® silicas are well defined, high purity, totally porous 

separation media that meet the most demanding needs of 

preparative and process scale users. Proven in over two 

decades of applications for protein, peptide, and nucleic 

acid separations, Vydac ® 300Å reversed-phase media 

have excellent selectivity and reproducibility. Bulk Vydac ® 

adsorbents incorporate bonded phase chemistries identical 

to those in Vydac ® brand analytical and prep HPLC columns, 

thereby assuring economical method development and 

reliable, dependable scale-up for preparative and process 

chromatographic applications. 

Vydac ® TP 300A Silica Specifications 

Pore Size: 

300Å 

Pore Volume: 

0.6mL/g 


Particle Shape: 

Spheroidal 

Vydac ® 300Å Media 

MS 

TP 

Butylsilane Octylsilane Octadecylsilane Butylsilane Octylsilane Octadecylsilane Octadecylsilane Diphenylsilane 

Bonded Chemistry 

C4 

214MS 

C8 

208MS 

C18 

218MS 

C4 

214TP 

C8 

208TP 

C18 

218TP 

C18 monomeric 

238TP 219TP 

Silica 

101TP 

10–15µm Particles 214MSB1015 208MSB1015 218MSB1015 214TPB1015 208TPB1015 218TPB1015 238TPB1015 219TPB1015 101TPB1015 



Available in 10g increments. 

tech tip 

Vydac ® MS vs. Vydac ® TP 

Both phases are based on the same high purity 300Å silica. However, MS undergoes an additional treatment prior to bonding that 

reduces the residual polar activity of the silica surface. For many applications this results in improved resolution and an increase 

in protein recovery. 

prep | media 

Two-Stage Purification of a Synthetic 135-mer 

Although generally recommended for oligonucleotides 

up to 75 bases, Vydac ® 214TP columns have been used 

to purify much longer synthetic oligonucleotides. Here 

a 135-mer is purified by two stages of chromatography 

on a 214TP column—the first with the 5'-dimethoxytrityl 

protecting group still attached, causing strong retention, 

and the second after removal of the trityl group. 

 

 

 

Column: Vydac ® 214TP1010 C4, 10µm,10 x 250mm 


Mobile Phase: A: 0.1 M Triethylammonium Acetate, pH 7.0 


Gradient: Crude: 0 to 60% B from 5 to 40min 

Deprotected: 0 to 20% B from 5 to 25min 


 

 

 

 

 

 

 

 

 

 

Data courtesy of Joseph Kosmoski and Dr. Michael Smerdon, Dept. of Biochemistry and Biophysics, Washington State University, Pullman, WA, USA 


Looking for Vydac ® analytical columns? 



177


Davisil ® Media 

• High surface area 

• Tight particle size distribution 

• Wide range of pore sizes (30–2500Å) and particle sizes (10–2000µm) available 

• Relied on for over 25 years 

Recognized worldwide as media of high purity, Davisil ® silica is the cornerstone of Grace’s offering 

of silica media products. Consisting of irregularly shaped particles with excellent mechanical and 

structural properties, Davisil ® silicas are versatile, consistent, and reliable. They are ideal for HPLC, 

solid phase extraction, flash chromatography, and scale-up to industrial process applications. 

Higher Performance 

Davisil ® silica’s chemical and structural properties are 

optimized for chromatographic performance. Tight control 

of these properties from raw material to finished product 

distinguishes Davisil ® silica from the competition.* 

High Surface Area for Increased Loading Capacity 

Synthetic Intermediate Purification 

prep | media 

Company Surface Area Bulk Density Surface Area of 1L Column 

Grace 550m 2 /g 420g/L 231,000m 2 /L 

X 515m 2 /g 430g/L 221,450m 2 /L 

Y 460m 2 /g 430g/L 197,800m 2 /L 

Z 450m 2 /g 450g/L 189,000m 2 /L 

High Purity Silica to Reduce Unwanted Interactions 

and Contamination 

Company Mg Ca 

Grace 25ppm 19ppm 

X 27ppm 207ppm 

Y 119ppm 793ppm 

Z 212ppm 1775ppm 

Tight Particle Size Distribution to Optimize 

Efficiency and Pressure Drop 

diff. Volume (%) 

18 

16 

14 

12 

10 

8 

6 

4 

2 

GRACE 

Most Narrow Particle 

Size Distribution 

100% 

Pet Ether 

Sample: 

1g Reaction Products 

Column: 

50 x 500mm Minutes 

Column Packing: Davisil ® LC60Å 20 – 45µm 

Mobile Phase: See Chromatogram 

Flow Rate: 175mL/min (535cm/hr) 

Detection: UV at 254nm 

Davisil ® silica purification of a schiff base from a crude reaction 

mixture containing aldehyde, amine and other by-products. The good 

separation and loading capacity shown at the pilot scale allowed 

scale-up to a 300mm diameter column producing over 90g of purified 

product per run. 

Global Technical Support 

80/20 

Pet/Diethyl Ether 

0 10 20 30 Min. 

To assist customers, Grace offers advice, assistance or 

laboratory trials for intended process-scale use. Our field 

representatives can arrange for such support when required, 

as well as discuss our ability to customize grades tailored to 

your requirements. 

0 

1 10 

100 

Particle size (µm) 

Grace X Y Z 

*All comparative data generated on chromatographic silica labeled 60Å, 40–63µm. 




Greater Selection 

A wide range of Davisil ® silica grades are available to meet 

your performance and economic requirements. The selection 

guide in this catalog will help you choose the best grade for 

your application. 

• Available in both normal phase bare silica and various 

bonding chemistries (C18, Amino, Diol, Cyano) for 

alternative selectivity 

• Wide selection of distinct pore diameters (30–2500Å) for 

separation of various MW sizes 

• Available from 500g to multi-ton quantities 

Davisil ® normal-phase silica functions through hydrophilic 

interactions, with more polar compounds generally retained 

longer. This makes it ideally suited for purification of: 

Chemical Synthesis 

Intermediates 

Silica 

Look for this icon on products from Grace using 

Davisil ® silica, such as TLC plates and SPE/ 

Flash cartridges, to experience the same great 

performance. 

Predictable Scale-Up 

Today we manufacture hundreds of tons of Davisil ® 

chromatographic silica per year in multi-ton lots. Our 

manufacturing is at scale, so your manufacturing can be 

at scale. In scaling up, you can be confident that Davisil ® 

chromatographic silica will yeild consistent performance 

as particle size is increased. 

Oils and Fats 

prep | media 

CAPACITY FACTOR (k’) 

3 

2.8 

2.6 

2.4 

2.2 

2 

1.8 

1.6 

1.4 

1.2 

1 

0.8 

0.6 

0.4 

0.2 

0 

0 15 

Dinitrobenzene 

Cholesterol 

N. Methylanine 

Phenol 

Benzaldehyde 

25 35 45 

Particle Size (µm) 

Uniform capacity (k ’ ) and selectivity (k ’ ) factors across all particle 

sizes for predictable scale-up. 

Unbeatable Product Reliability 

Natural Products 

(vitamins, flavors, 

fragrances, etc.) 

Manufactured for over 25 years, Davisil ® chromatographic 

silica is one of the world’s most widely used chromatography 

sorbents. 

Surface Area 

& Pore Volume: 

+/-10% lot to lot 

Produced at two ISO-9001 certified facilities under strict 

QC controls from raw material to finished product insures 

high lot-to-lot reproducibility. 


179



Selection Guide 

Many types of column packings are suitable for a given application. The diagram below is intended for general guidance 

to the chromatographer. By following each of the three paths in sequence, the proper packing media is selected. 

It should be noted that Grace offers other media to complement the Davisil ® packings in many of these applications. 

Step 1 Step 2 Step 3 

Sample 

Molecular 

Weight 

Sample 

Separation Difficulty 

& System Pressure 

Limitations** 

Sample 

Solubility 

prep | media 



Below is a listing of our more popular Davisil ® silica grades. 

For a complete listing request brochure 538. 

Unbonded Silica 

APS Particle Size Davisil ® Silica Grade Pk Size Part No. 

30Å 50–100µm 921 25kg 5101737 

75–150µm 923 1 25kg 5138973 

5lb 5139002 

72 x 10g 5139003 

576 x 10g 5139001 

75–700µm 12 2 25kg 5139180 

250–500µm 15 3 25kg 5101744 

60Å 10µm 710NW 20kg 5136220 

16–20µm 631 25kg 5101778 

40–63µm LC60Å 40–63µm 25kg 5054993 

5kg 5098468 

1kg 5134312 

60–200µm LC60Å 60–200µm 25kg 5007446 

5kg 5134295 

1kg 5134311 

200–500µm LC60Å 200–500µm 25kg 5022298 

150Å 16–24µm LC150Å 16–24µm 25kg 5018962 

35–70µm LC150Å 35–70µm 25kg 5057993 

5kg 5134294 

1kg 5134299 

60–200µm LC150Å 60–200µm 25kg 5134770 

5kg 5134293 

1kg 5134298 

250Å 40–63µm LC250Å 40–63µm 15kg 5134301 

2.5kg 5134292 

60–200µm LC250Å 60–200µm 15kg 5134300 

2.5kg 5134291 

500Å 35–70µm XWP500Å 35–70µm 20kg 5030057 

5kg 5108711 

90–130µm XWP500Å 90–130µm 20kg 5058842 

5kg 5134305 

100–300µm XWP500Å 100–300µm 20kg 5057050 

1000Å 16–24µm XWP1000Å 16–24µm 20kg 5134302 

5kg 5016756 

35–70µm XWP1000Å 35–70µm 20kg 5034754 

5kg 5134304 

90–130µm XWP1000Å 90–130µm 20kg 5093501 

5kg 5134303 

1500Å 16–24µm XWP1500Å 16–24µm 18kg 5070159 

90–130µm XWP1500Å 90–130µm 18kg 5045916 

2500Å 90–130µm XWP2500Å 90–130µm 5kg 5107451 

ASTM or EPA Method 

1 

Grade 923 ASTM D1319: Petroleum Products by FIA 

ASTM D2549: Aromatics/Non-Aromatics from Oils 

EPA Method 1664: N-Hexane Extraction Method 

2 

Grade 12 ASTM D2007: Rubber Extender/ Processing Oils 

3 

Grade 15 EPA Method 601/624: Purgeable Halocarbons 

Bonded Silica 

APS Bonded Phase Particle Size Davisil ® Silica Grade Pk Size Part No. 

60Å C18 10µm 710NC18E 250g 5135418 

1kg 5135305 

35–60µm 633NC18E 250g 5135414 

1kg 5134095 

Cyano 10µm 710NCNE 250g 5135419 

1kg 5134223 

35–60µm 633NCNE 250g 5135415 

1kg 5134224 

Diol 10µm 710N2OH 250g 5135417 

1kg 5135303 

35–60µm 633N2OH 250g 5135413 

1kg 5135302 

Amino 10µm 710NNH2 250g 5135420 

1kg 5134682 

35–60µm 633NNH2 250g 5135416 

1kg 5134096 


Matching Davisil ® Silica TLC plates for easy method development. 

Davisil ® Silica TLC Plates 

Description Layer Thickness Qty. Part No. 

Hard Layer, Organic Binder, Fluorescent Indicator, 254nm 

Scored, 4, 5 x 20cm Sections 

20 x 20cm 250µm 25 8617580 

Scored, 8, 2.5 x 10cm Sections 

10 x 20cm 250µm 25 8617610 

7174 

prep | media 


Column Hardware and Packing 

Equipment also available. 



Looking for additional TLC products? 



181


prep | accessories 

Stainless Steel Tubing and Fittings 

Tubing and Fitting Kits for MODcol ® Flanged and Spring Columns 

Tubing and Fitting Kits 

Description 

Part No. 





*1 union, 1 nut, 1 ferrule, and 2 plugs. Compatible with flanged and Spring Columns up to 25mm i.d. 

**1 union, 1 nut, 1 ferrule, 2 plugs, and 2 female reducers 1/8” to 1/16.” Compatible with flanged and Spring Columns of 30mm i.d. and greater. 

Valco ® High-Pressure Fittings 

Valco ® Fitting Specifications 

Material: 


Max. Pressure: 

Typical Use: 

A 


G 

J 


10,000psig 



High-Pressure Tubing Connections 

5431 5697 

B 



Stainless Steel Nuts 

A 1/16" Nut 10 306121 

A 1/8" Nut 10 306151 

Stainless Steel Ferrules 

B 1/16" (Type 316) 10 30646 

B 1/8" (Type 316) 10 30647 

5511 5512 

I 

5520 

5521 5550 


Internal Unions 

G 1/16" 0.50mm 30714 

G 1/16" 0.75mm 30715 

G 1/16" 1/16" 45835 

G 1/8" 2.0mm 30718 

G 1/8" 1/8" 30719 

External Unions 

H 1/8" 2.0mm 30731 

Internal Reducing Unions 

I 1/8" to 1/16" 0.25mm 30746 

I 1/8" to 1/16" 0.75mm 45850 

I 1/4" to 1/8" 1/8" 30755 

External/Internal Reducing Unions 

J 1/8" to 1/16" 1/16" 30761 

J 1/4" to 1/8" 2.0mm 30765 

J 1/4" to 1/8" 1/8" 30766 

Column Endfittings 

K 1/4" to 1/16" 2.1mm 45905 

K 1/4" to 1/16" 3.0mm 45907 

H 

K 

Hi-EFF Grade 

Stainless Steel Tubing 

Hi-EFF grade stainless steel 

tubing is especially tempered 

for easy bending and is 

washed with acetone to remove any residual materials. 

Hi-EFF Stainless Steel Tubing Specifications 

Material: 

316 Series Stainless Steel 


Maximum Pressure: Varies according to i.d.* 

Typical Use: 

High-Pressure Plumbing 

*Request datasheet 3005D for more information. 

Hi-EFF Grade Stainless Steel Tubing 

Per Ft** Per Meter 50ft Coil 

o.d. 

i.d. 

Part No. Part No. Part No. 

Type 316 Stainless Steel 

1/8" (3.18mm) 0.085" (2.16mm) 30105 30107 30109 

1/4" (6.35mm) 0.210" (5.33mm) 30307 30308 30309 

**Minimum tubing order is 3'. 


This Stainless Steel Tubing is the same high quality as the 

Hi-EFF grade, but has not been washed with acetone. 

Stainless Steel Tubing Specifications 

Material: 




Typical Use: 


*Request datasheet 3005D for more information. 


Per Ft** 

Part No. 


For additional information on 

HPLC fittings see pages 112–122, 

and for tubing information see pages 

384–391. 

Per Meter 

Part No. 

50ft Coil 

Part No. 

200ft Coil 

Part No. 

o.d. 

i.d. 

Type 316 Stainless Steel 

1/16" 0.020" 3002100 300230 3002 300220 

(1.59mm) (0.51mm) 

0.040" 30031 30033 3003 — 

(1.02mm) 

**Minimum tubing order is 3'. 

5537 



Pumps, Injectors, and Switching Valves 

Model 627 Preparative HPLC Pump 

Flow rate range of 0.1mL/min to 100.0mL/min. 

Ideal for Scale-up Work, Semi-Prep and 

Preparative HPLC 

• Dual piston mechanism for pulse-free operation 



• Isocratic or high-pressure binary gradient 

The Model 627 Preparative Pump delivers the accuracy and 

precision of a standard analytical pump at flow rates up to 

100mL/min. Consistent flow rates and pressures minimize 

the potential for column damage. The 627 pump is available 

in 316 stainless steel or biocompatible PEEK. 

Buy one pump now and upgrade to a binary system later by 

purchasing a second pump and a high-pressure gradient kit. 

Model 627 HPLC Pump Specifications 

Max. Operating Pressure: 4000psig (both SS and PEEK) 

Flow Rate Range: 

0.1–100.0 mL/min 

Flow Rate Precision: 0.5% RSD 



Gradient Profiles: 



Dimensions: 

6" H x 11" W x 18" D 


Weight: 

35lb (16kg) 

Warranty: 

1-year parts, 1-year labor, excluding seals 

and check valves 

Model 627 Preparative Pump 

Description 

Part No. 


PEEK, 85-265VAC, 47-63Hz 627150 

Stainless Steel, 85-265VAC, 47-63Hz 627250 

Binary Gradient System 

(Includes 2 pumps, system controller, mixing tee, tubing and fittings) 

PEEK, 85-265VAC, 47-63Hz 627350 

Stainless Steel, 85-265VAC, 47-63Hz 627450 

Kits and Accessories 

Description 

Part No. 

Accessories 


105625 



105628 




Piston 121504 


Check Valve Kit, Metal-Free 060141 

Note: All replacement parts kits contain parts for one pump head only. 

6774 

Rheodyne ® Model 3725 Prep-Scale Injectors 

• Sample range 100µL to 20mL 

• Flow rates from 10 to 

800mL/min 

• Uses 16-gauge needle 

• 1.0mm (0.04") flow passages 

These injectors are used with 

preparative HPLC columns from 

1 to 10cm diameter. They have 

the ease of use and versatility 

of Rheodyne’s analytical-scale sample injectors, plus the 

capacity for large sample volumes and flow rates. 

The Model 3725 Valves are available in stainless steel or 

PEEK, and with a built-in position sensing switch (Model 

3725i-038 or 3725i). Each model is supplied with a 10mL loop 

and 1/8" fittings must be used. An optional port adapter can 

be used to connect 1/16" tubing. Suitable for pressures up to 

5000psig. 

Rheodyne ® Prep-Scale Sample Injectors 


Prep Injection Valves 

Model 3725 PEEK 37250 

Model 3725-038 SS 37255 

Model 3725i-038 SS 37256 

Accessory 

Port Adapter for 1/16" tubing — 600760 


• Inexpensive manual 

switching valve 

• Wide-bore version for prep 

Rheodyne ® HPLC Switching 

Valves offer an endless variety 

of ways to simplify switching and 

injecting. Standard valves are 

supplied with 0.024" internal flow 

passages and are suitable for use 

with analytical and semi-preparative applications. Large bore 

valves with 0.040" internal flow passages are available for 

use at high flow rates (greater than 50mL/min) 7000psig 

pressure limit. 

A spring detent mechanism included in the six-position 

selection valve permits full circle rotation in either direction 

and ensures that the rotor “falls into” each of the six positions 

at the precise 60° spacing. 


Description Version Bore Part No. 


6-Port* SS Standard 7000RV 

6-Port* SS Large Bore 7000L 

10-Port* SS Standard 7610400 

10-Port* PEEK Standard 7610600 






6-Position 6-Port SS Standard 7060RV 

6-Position 6-Port SS Large Bore 7060L 

*5000psig pressure rating for 2-position 6-Port and 10-port. 

5495 

5619 


prep | accessories 

183


Flash Chromatography Introduction 

The technique of flash chromatography has advanced 

considerably since it began in the 1970s with self-packed 

glass columns that relied on gravity for flow. Instrumentation 

today automates and speeds the purification, and pre-packed 

disposable cartridges eliminate the time spent packing. 

However, the one area of flash chromatography that has seen 

little advancement is the heart of the separation, the silica 

Experiment 

Loading Capacity Study 

The loading capacity of a GraceResolv 40g cartridge and 

a leading competitor’s 40g cartridge were compared using 

the purification of a sample mixture containing Dimethyl 

Phthalate and Toluene as an example. The testing determined 

maximum loading capacity with baseline resolution. 

Flash Silica Technology 

Most cartridges today are packed with the same 40–63µm 

irregular shaped silica that was used for self-packed glass 

columns in the 1970s. The benefit of this type of silica is 

that it is inexpensive and generates low backpressure while 

providing a marginal level of resolution. Grace, as a silica 

manufacturer, supplies this same grade of silica to many of 

the flash manufacturers. However, as a silica manufacturer, 

and a company with a breadth of chromatography products 

and knowledge, Grace is advancing the field of flash 

chromatography by introducing innovative silica and cartridge 

manufacturing. 

Figure 1. 

Loading Capacity Comparison 

GraceResolv 

0 1 2 3 4 5 6 Min. 

Amount of 

Sample Loaded 

0.48g 

3.84g 

6.72g 

9.12g 

9.6g 

GraceResolv Silica 

flash 

Grace developed a new grade of Davisil ® silica 

that dramatically increases resolution and 

capacity without an increase in backpressure. 

Silica 

The silica used in the GraceResolv cartridges 

is smaller in particle size than traditional flash 

silicas but has a more narrow particle size distribution and 

very few fine particles. As in HPLC, the smaller particles 

generate greater efficiencies in the packed column, increasing 

resolution. The narrow particle size distribution allows the 

cartridge to be packed with higher efficiencies and the 

reduction in fines allows all of this to happen without a change 

in backpressure. 

Competitor 

0 1 2 3 4 5 6 Min. 

Cartridge: 40g silica cartridge 

Sample: Peak 1: Toluene 

Peak 2: Dimethyl Phthalate 

Mobile Phase: Hexane:Ethyl Acetate (70:30) 



GraceResolv silica also undergoes a proprietary process to 

remove metals from the silica surface. Surface metals cause 

mixed-mode interactions that lead to peak tailing and a loss of 

resolution. GraceResolv silica has 50% less surface metals 

than standard irregular silica used today in flash cartridges. 

This makes a dramatic difference in resolution especially for 

metal chelator compounds. 

GraceResolv High-Resolution Flash 

Cartridges 

The advantage of the silica used in our cartridges is 

guaranteed with a rigorous quality assurance program. 

Each GraceResolv cartridge undergoes over 15 tests, 

from silica to packed cartridge, to ensure unvarying lot-to-lot 

performance. A chromatographic test is performed to ensure 

that the columns are well packed and meet our efficiency, 

resolution, and peak symmetry specifications. 

Figure 2. 

Maximum Loading Capacity with Baseline Resolution 

GraceResolv 9.12g 

0 1 2 3 4 5 6 Min. 

Competitor 3.84g 

7280 

0 1 2 3 4 5 6 Min. 

Results 

This experiment demonstrates how the improved silica 

technology in GraceResolv cartridges and efficient column 

packing improve the resolution of the separation and allow 

higher sample loading at high product purity. 



GraceResolv High-Resolution Flash Cartridges 

• High resolution 

• Increased loading capacity 

• Reproducible performance 

• Compatible with all flash instruments 

• Novel silica 

Grace’s 30 years of chromatography experience 

combined with 80 years of silica manufacturing 

expertise has yielded the ideal flash cartridge, 

Silica 

GraceResolv. GraceResolv cartridges 

are packed with a new grade of Davisil ® 

silica designed to maximize performance of today’s flash 

instruments. 

Increase Resolution and Product Purity with GraceResolv Flash Cartridges 

(patent pending) 

1.00 

0.90 

Leading Competitor Cartridge 

1. Acetylacetone 

H 3 C 

CH 3 

1.00 

0.90 

1 

GraceResolv Flash Cartridges 

0.80 

0.70 

0.60 

compound 

not 

resolved 

2 

O O 

2. Methyl Paraben 

O 

HO 

0.80 

0.70 

0.60 

2 

pure 

fraction 

completely 

resolved 

0.50 

OCH 3 

0.50 

0.40 

0.30 

0.20 

0.10 

0.00 

0 

1 

1 

2 

3 4 Min. 

Cartridge Size: 

Instrument: 

Sample: 

Mobile Phase: 

Flow Rate: 

Detector: 

12g 

Combiflash ® Companion ® /TS 

Acetylacetone, Methyl 

Paraben dissolved in 95:5 

Hexane: IPA with 1% TFA 

80:20 Hexane:Ethyl Acetate 

36mL/min 

UV at 254nm 

0.40 

0.30 

0.20 

0.10 

0.00 

0 

1 

2 

3 4 Min. 

flash 

What Gives GraceResolv Cartridges Their Superior Performance? 

1. Small Particle Size 

A smaller particle size increases resolution. Tighter particle 

size distribution and minimal fines keep backpressure low. 

See Figure 1. 

2. High Purity Silica 

Grace’s flash silica undergoes a proprietary process to 

reduce metals from the silica surface by over 50%. Metals 

cause peak tailing and reduce resolution especially for metal 

chelator compounds. 

Percentage of Metal Content 

Silica Fe2O3 Na2O Al2O3 SO4 

GraceResolv 0.001 0.01 0.02 0.001 

Competitive Standard 0.005 0.07 0.05 0.01 

Particle Size Distribution 

GraceResolv Flash Cartridges 

Standard Flash Silica 

1 10 100 1000 2000µm 

Measured by Malvern ® Mastersizer ® 2000. 

Figure 1 

3. Efficient Column Packing 

A tightly packed silica bed increases cartridge efficiency and 

prevents voids and channeling that cause poor resolution. 

Flash chromatography, like HPLC, benefits greatly from 

optimized packing procedures. See Figure 2. 

4. Strict Quality Control 

GraceResolv cartridges are characterized with over 

15 tests, from the silica to the packed cartridge, to ensure 

unvarying lot-to-lot performance. A quality assurance 

certificate is provided with each box of cartridges. 

0.06 

0.04 

0.02 

0.00 

0 

Figure 2 

Cartridge Efficiency Chromatogram 

6600 plates/meter 

1700 plates/meter 

5 10 15 Min. 

Results from identical silica packed into a 330g cartridge. 


185


GraceResolv Silica Cartridges—Luer Endfittings 

Directly Connect to Isco ® Systems 

7278 

GraceResolv Cartridges—Luer Endfittings 

flash 

GraceResolv Cartridges—Luer Endfittings 

Cartridge Size Qty. Part No. 

GraceResolv 4g 20/pk 8618502 






Solid Loaders 

Empty* 

Silica Filled 

Description Qty. Part No. Qty. Part No. 

Luer Slip Solid Loader—Isco ® Compatible 

5g (12mL cartridge) 100/pk 5142050 20/pk 5142035 


Luer Lock Solid Loader—Biotage ® Compatible 



*Empty solid loaders come with two 20µm frits. 

Luer Lock Solid Loader 

7376 7375 

Luer Slip Solid Loader 

Adapters to Connect to Biotage ® Systems 

Support Rings 

7350 

Adapters 

Support Rings 

Description 

Part No. 

Pack contains adapters for five sizes (4, 12, 40, 80, 120g) 8618945 

Adapters 

Description 

Part No. 

Male and female luer solvent line connectors 8618940 

Adjustable Plunger 

Description 

Part No. 

5g (12mL cartridge) 8619011 

25g (60mL cartridge) 8619010 

7338 

tech tip 

To connect a GraceResolv cartridge to 

a Biotage ® instrument, simply connect 

the adapters to the inlet and outlet 

side of the cartridge and attach to the 

instrument’s tubing. The cartridge then 

fits on the Biotage ® instrument with 

the appropriate support ring. 

If needed, a luer lock solid loader 

can also be used with the 

adjustable plunger. 

Adjustable Plunger 

7402 



GraceResolv Silica Cartridges—SPE Style 

Directly Connect to FlashMaster Systems 

GraceResolv Cartridges—SPE Style 

Cartridge Size Qty. Part No. 

GraceResolv 5g/25mL 20/pk 8618508 






GraceResolv Cartridges—SPE Style 

7349 

GraceResolv Silica TLC Plates and Tanks 

• TLC plates use the same silica as the GraceResolv 

cartridges for direct method development 

• Plates are pre-scored in 5 x 20cm sections for easy size 

customization 

flash 

GraceResolv Silica TLC Plates 

Description Thickness Qty. Part No. 


20 x 20cm (Scored into four 5 x 20cm sections) 250µm 25 8618900 

Rectangular TLC Tanks with Glass Lid 

Description 

Part No. 

Standard Tank for 20 x 20cm Plates, (27x24x7cm), (10.5x10x3") 2108359 

Davisil ® Bulk Silica 

Standard flash grade silica available for solid loading, 

scale-up to large-scale process applications, and 

self-packing of flash cartridges. 

TLC Plates 

Rectangular TLC Tank 

5369 5346 

more info 

Additional TLC accessories and Davisil ® silica grades are 

available from Grace. Refer to the TLC section on pages 188–198 

and Media section on pages 175–181. 

Silica 

Davisil ® Bulk Silica 

APS Particle Size Davisil ® Grade Pk Size Part No. 

60Å 40–63µm LC60Å 40–63µm 25 kg 5054993 

5 kg 5098468 

1 kg 5134312 

60–200µm LC60Å 60–200µm 25 kg 5007446 

5 kg 5134295 

1 kg 5134311 

150Å 35–70µm LC150Å 35–70µm 25 kg 5057993 

5 kg 5134294 

1 kg 5134299 

60–200µm LC150Å 60–200µm 25kg 5134770 

5 kg 5134293 

1 kg 5134298 

7115 

Davisil ® Bulk Bonded Silica 

APS Bonded Phase Particle Size Davisil ® Grade Pk Size Part No. 

60Å C18 35–70µm 633NC18E 250g 5135414 

1kg 5134095 

Cyano 35–70µm 633NCNE 250g 5135415 

1kg 5134224 

Diol 35–70µm 633N2OH 250g 5135413 

1kg 5135302 

Amino 35–70µm 633NNH2 250g 5135416 

1kg 5134096 


187


tlc 

TLC Introduction 

Thin Layer Chromatography to Preparative 

Chromatography 

One of the first steps in scale-up of preparative liquid 

chromatography separations is selection of an appropriate 

mobile phase. Two methods are commonly used to 

determine the proper mobile phase composition: Thin 

Layer Chromatography (TLC) or High Performance Liquid 

Chromatography (HPLC). The use of TLC will be discussed 

here to deal with the successful correlation between the TLC 

separation to the preparative silica column. 

TLC is a liquid-solid adsorption technique where the mobile 

phase ascends the thin layer of stationary phase coated onto 

a backing support such as glass by capillary action. There 

is a similar relationship to column chromatography where 

the solvent travels down through the column’s adsorbent. 

The similar relationship allows TLC to be a rapid method for 

determining solvent composition for preparative separations. 

Steps for Method Development 

Choose Stationary Phase 

Choose a scalable TLC plate, preferably that has an identical 

media as the preparative column. Choose between normal 

and reverse phase based on sample polarity and solubility. 

Choose a Mobile Phase 

Criteria for Choosing a Preparative Solvent 

• Solubility 

• Affinity 

• Resolution 

1. Solubility 

Many solvent systems provide the minimal solubility for the 

sample, but to elute a sample from a column the mobile 

phase must have a greater solubility for the sample, as the 

sample concentration is usually very high. When possible, it 

is best to dissolve the sample in the mobile phase. The first 

step in solvent selection is determination of the solubility of 

the sample. The desired mobile phase would provide the 

greatest solubility, while providing affinity for the sample on 

the stationary phase. 

Solvent Solubility Screening Table 

Water 

Methanol 

Ethanol 

Acetone 

Diethyl Ether 

Ethyl Acetate 

Dichloromethane 

Toluene 

Chloroform 

Cyclohexane 

Petroleum Ether 

Hexane 

Increasing Polarity 

2. Affinity 

To achieve a separation, the sample must have a relatively 

equal affinity for the solvent and the packing material. If the 

sample has a higher affinity for the stationary phase than the 

solvent, the sample will remain at the origin (Rf value will be 

too low). 

3. Resolution 

Resolution is improved by optimizing the affinity between 

sample, solvent, and support. The optimum solvent for 

separating two or more compounds will maximize the 

difference in the compounds. Most TLC and preparative 

mobile phase systems contain a polar solvent and a 

chromatographically dissimilar less-polar solvent. As a 

guide for method development, a substitution in the polar 

solvent often results in a change in resolution, while a change 

in the less-polar solvent results primarily in a change in Rf 

of the sample components. The table below shows some 

common tendencies of various functional groups to adsorb 

onto the silica. 

Affinity of Functional Groups for Silica Gel 

-NH2 Amine 

-COOH Carboxylic Acid 

-COH Alcohol 

-CONH2 Amide 

-C=O Carbonyl 

-C=-CO2R Ester 

-C-O-C Ether 

-C1 Halocarbons 

-CC- Hydrocarbons 

Select Visualization Technique 

Once a mobile phase is selected, visualization techniques 

will need to be determined. Common techniques include 

SWUV, I2 /SWUV, I2 /KI for Nitrogen containing compounds, 

H2SO4/LWUV, H2SO4/PMA for non-nitrogen containing 

compounds. 

Perform TLC Analysis 

Increasing Affinity 

Look up the affinity for the type of compound as well as 

the solvent strengths to find a starting point for method 

development or look up a reference from a similar structure, 

then adjust the mobile phase composition to adjust the Rf. It 

is common to try 3–6 solvent systems for the first round of 

method development. Review the results after visualization 

and adjust the Rf if necessary, increase the separation and 

evaluate visualization techniques to make sure you are seeing 

all necessary compounds. 

Optimizing TLC Separations for Preparative 

Separations 

The optimum separation of compounds by TLC is usually 

achieved when Rf values are between 0.3–0.5. 

Rf = Distance from origin to center of spot 

Distance from origin to solvent front 

Generally, adjusting the compound’s Rf between 0.3–0.5 is 

done first for a TLC separation. For scale-up to preparative 

separations, the TLC solvent system’s polarity must be 

decreased to lower the Rf between 0.15–0.35. This Rf range 

is optimal for a preparative separation, in terms of sample 

load, resolution, residence time, and solvent usage. 

Determination of Column Volumes (C.V.) 

The equation C.V. = 1/ Rf relates the TLC values and the 

preparative LC column volumes to elute each component. 

This equation is only a guideline and the relationship 

between the Rf values and the column volume will vary in 

use. Generally, the LC column volumes will be equal to or 

less than the calculated values. The elution volume will also 

be dependent upon the sample load and solvent used to 

solubilize the sample. 



TLC Plates 


• Made with the same Davisil ® silica as sold in 

bulk for easy method development 

• Scored to customize to your plate size preference 

Silica 





20 x 20cm 250µm 25 8617580 

Scored, 8, 2.5 x 10cm Sections 

10 x 20cm 250µm 25 8617610 

Alltech ® Prep-Screen TLC Plates 

• Made with the 10–12µm spherical silica gel used for 

Alltech ® prep-HPLC cartridge columns 

• Available in both Econosphere silica gel and C18 

(reversed-phase) bonded versions 

Prep-Screen HPTLC Plates help to optimize sample 

separation parameters for use in preparative HPLC. These 

plates allow a quick, inexpensive preview of sample traits in 

various mobile phase systems prior to HPLC analysis. The 

adsorbent is the same Econosphere silica or C18 used in 

Alltech packed HPLC columns. 


• Made with the same high-purity Davisil ® silica as used 

in the GraceResolv flash cartridges for easy method 

development 

• Scored to customize to your plate size preference 





20 x 20cm 250µm 25 8618900 

Prep-Screen C18 

3 

2 

1 

7087 

1. Progesterone 

2. Testosterone 


Prep-Screen Sol. System: 

Methanol:Water (70:30) 

HPLC Sol. System: 

Methanol:Water (70:30) 

Econo-Prep HPLC 

3 

7065 

2 

1 

tlc 

Prep Screen HPTLC Plates 


Prep-Screen HPTLC Plates, 10 x 10cm, 200µm Layer 

Silica Gel, 10µm Spherical Adsorbent 10 16328 

C18, 10µm Spherical Adsorbent 10 16332 

3179 

Grace has a large 

selection of TLC plates 

to suit your separation 

needs. 

5369 






See pages 194–198 for tanks, sprayers, applicators, and other 

TLC accessories. 


189


TLC Plates 

Alltech ® Prekotes 

• High purity silica gel for increased sensitivity 

• Controlled particle size for faster separations and 

improved resolution (>80% in 6–15µm range) 

• Precoated plates provide convenience and superior 

layer quality 

Adsorbosil ® HPTLC Plates 

• 150µm layer thickness for fast, high resolution separations 

• Smooth surface for noise-free densitometry 

HPTLC with Organic Binder 

• Organic binder makes an abrasion-resistant layer 

• Use up to 80% water in the solvent system 

• Maximum temp for charring is 150°C 

Adsorbosil ® -Plus 

Silica Gel H 

(Soft Layer) 

+ CaSO 4 

Adsorbosil ® -Plus 1 

Silica Gel G 

(Soft Layer) 

HPTLC with Inorganic Binder 

• Allows use of 100% water in the solvent system 

• Use when strong charring is required 

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Soft Layer 

• Contains no organic binders 

• Sample zones are easy to remove for further analysis 

Soft Layer Adsorbosil ® Prekotes 


Soft Layer Prekotes, Glass-Backed 

Conventional, 250µm Layer Thickness, 20 x 20cm 

Adsorbosil ® -Plus 25 16384 

Adsorbosil ® -Plus P* 25 16376 

Adsorbosil ® -Plus 1 25 16330 

Adsorbosil ® -Plus 1 P* 25 16322 

Preadsorbent, 250µm Layer Thickness, 20 x 20cm 



Preadsorbent/Prechannel, 250µm Layer, 20 x 20cm 



Preparative, 500µm Layer Thickness, 20 x 20cm 


*P = With Fluorescent Indicator, 254 wavelength. 

Hard Layer 

• Contains a proprietary inorganic binder to add abrasion 

resistance to layer 

• Use when strong charring is required for visualization 

• Write on layer with pencil or felt pen 

• Sample zones can be removed for further analysis 

High-Performance TLC Plates 


Adsorbosil ® -Plus Plates with Organic Binder, 10 x 10cm 

Adsorbosil ® -Plus HPTLC 25 16405 

Adsorbosil ® -Plus P, HPTLC, F254 25 16420 

Adsorbosil ® -Plus HPTLC with 

25 16402 

Preadsorbent Zone 

Adsorbosil ® -Plus P HPTLC with 

25 16403 

Preadsorbent Zone, F254 

Adsorbosil ® -Plus 1 Plates with Inorganic Binder, 10 x 10cm 

Adsorbosil ® -Plus 1 HPTLC 25 16400 

Adsorbosil ® -Plus 1P HPTLC, with F254 

UV Indicator 

25 16401 

Reversed-Phase HPTLC Plates 

• Coated with non-polar, C18 chemically bonded support 

• Cross-linked organic polymer binder makes an abrasion 

resistant layer and a smooth surface 

• Use to separate mixtures of lipophilic components, 

hydrocarbons, fats and waxes, fat soluble vitamins 

or steroids 

Fatty Acid Separation 

1. Methyl Oleate 

2. Methyl Linoleate 

3. Methyl Linolenate 

7088 

Hard Layer Adsorbosil ® Prekotes 


Hard Layer Prekotes, Glass-Backed 

Conventional, 250µm Layer Thickness, 20 x 20cm 



Preadsorbent, 250µm Layer Thickness, 20 x 20cm 



Preadsorbent/Prechannel, 250µm Layer, 20 x 20cm 



*P = With Fluorescent Indicator, 254 wavelength. 

1 2 3 

TLC Plate: Adsorbosil ® RP HPTLC (Part No. 16314) 

Sol. System: Acetonitrile:Acetic Acid:Water (70:10:1) 

Development Time: 25min 

Visualization: Iodine Vapor 

High-Performance TLC Plates 


Reversed-Phase C18 HPTLC, 150µm Layer Thickness 

Adsorbosil ® RP HPTLC, 10 x 10cm 25 16314 

Adsorbosil ® RP HPTLC Plates with F254, 25 16315 

10 x 10cm 

Adsorbosil ® RP HPTLC Plates, Prescored, 25 16318 

10 x 20cm 

Adsorbosil ® RP HPTLC Plates, Prescored, 

with F254, 10 x 20cm 

25 16319 



TLC Plates 

Macherey-Nagel 

Silica Gel Plates 

• 60Å pore size 

• Particle size, 5 to 17µm 

• Binder is organic, stable in most organic solvents and 

aggressive detection reagents 

ADAMANT Silica Layers for TLC 

• Increased UV brilliance for increased detection sensitivity 

• Extremely hard, dust-free surface 

• Optimized particle size distribution for improved 

separation efficiency 

• Ideal for trace analyses 

Glass-Backed Macherey-Nagel TLC Plates 

Description Size Layer Qty. Part No. 

Silica Gel Plates, Glass-Backed 

Conventional Layers, Silica Gel 60 

SIL G-25 5 x 10cm 250µm 50 809017 

SIL G-25 5 x 20cm 250µm 100 809011 

SIL G-25 20 x 20cm 250µm 25 809013 

SIL G-25 UV254 5 x 20cm 250µm 100 809021 

SIL G-25 UV254 20 x 20cm 250µm 25 809023 

SIL G-25 UV254+366 20 x 20cm 250µm 25 809123 

Preparative Layers 

SIL G-100 UV254 20 x 20cm 1000µm 15 809063 

SIL G-200 UV254 20 x 20cm 2000µm 12 809083 

ADAMANT Glass-Backed Plates 


ADAMANT UV254 2.5 x 7.5cm 250µm 100 821005 

ADAMANT UV254 10 x 10cm 250µm 25 821020 



Nano-Series HPTLC Plates 

• Particle size, 2–10µm 

• Higher speed 

• Better resolution and sensitivity than regular TLC plates 

Specialty Plates 

• Silica Gel and Cellulose—Specific for separation of 

food preservatives 

• Silica Gel—Specific for aflatoxin analysis 

• Aluminum Oxide—pH 9 with 60Å pore size, 

surface area = 200m 2 /gram 

Specialty Plates 


Specialty Plates, Glass-Backed 

Silica Gel and Cellulose — Mixed Layer 

SILCEL-Mix-25 UV254 20 x 20cm 250µm 25 810043 

Silica Gel Highly Purified with Gypsum 

SIL G-25 HR 20 x 20cm 250µm 25 809033 

SIL G-25 HR UV254 20 x 20cm 250µm 25 809043 

Aluminum Oxide, Basic 

ALOX-25 UV254 20 x 20cm 250µm 25 807023 

ALOX-100 UV254 20 x 20cm 1000µm 15 807033 


Performing flash separations? 

See GraceResolv flash consumable product line on 

pages 184–187. 

Nano-SIL HPTLC Plates 


Nano-Series HPTLC Plates, Glass-Backed 

Silica Gel 60 

Nano-SIL-20 10 x 20cm 200µm 50 811013 

Nano-SIL-20/UV254 10 x 20cm 200µm 50 811023 

Nano-Series HPTLC Plates, Aluminum-Backed* 

Silica Gel 60 

Nano-SIL G 20 x 20cm 200µm 25 818141 

Nano-SIL G/UV254 20 x 20cm 200µm 25 818143 

*Do not use with mineral acids or concentrated ammonia. 








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191


TLC Plates 

Macherey-Nagel Polygram ® Series 

Silica Gel 60 

• SIL G and SIL N layers have different binders and show 

different separation characteristics 

Cellulose, Avicel-Microcrystalline 

Cellulose MN 300—Native Fibrous Cellulose 

• Fibers are 2–20µm in length 

Hybrid Plates 

• “Wettable” RP plate 

• For both reversed- and normal-phase chromatography 

• Determine polarity by eluent selection 

• Activate layer at 110–115°C prior to use 

• Particle size of 2–10µm for improved separations 

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Cellulose, Ion-Exchanger 

• DEAE carries positive charges at neutral and acidic pH 

• Use DEAE to separate proteins, enzymes, and hormones 

• Use PEI to analyze nucleic acids 

Polyamide 6 

• Specific for separation of dansyl and DNP amino acids 

Polygram ® Flexible-Backed TLC Plates 


Polyester-Backed TLC Plates 

Silica Gel 60 

SIL G 20 x 20cm 250µm 25 805013 

SIL G/UV254 4 x 8cm 250µm 50 805021 

SIL G/UV 254 20 x 20cm 250µm 25 805023 

SIL N-HR/UV254 20 x 20cm 200µm 25 804023 

Cellulose Plates 

Cellulose MN 400, Avicel-Microcrystalline Cellulose 

CEL 400 20 x 20cm 100µm 25 801113 

CEL 400 UV254 20 x 20cm 100µm 25 801123 

Cellulose MN 300 

CEL 300 20 x 20cm 100µm 25 801013 

CEL 300 UV254 20 x 20cm 100µm 25 801023 

Cellulose, Ion-Exchanger 

CEL 300 DEAE 20 x 20cm 100µm 25 801073 

CEL 300 PEI* 20 x 20cm 100µm 25 801053 

CEL 300 PEI/UV254 20 x 20cm 100µm 25 801063 

Specialty Polyester-Backed Plates 

Polyamide 6 

POLYAMIDE 6 20 x 20cm 100µm 25 803013 

POLYAMIDE 6 UV254 20 x 20cm 100µm 25 803023 

Aluminum Oxide, Basic, 20µm Particle Size 

ALOX N/UV254 20 x 20cm 200µm 25 802023 

ALOX N/UV254 4 x 8cm 200µm 50 802021 

Aluminum Oxide, Basic, pH 9, Aluminum-Backed 

ALOX N 20 x 20cm 200µm 25 818013 

ALOX N/UV254 20 x 20cm 200µm 25 818023 

*PEI plates must be refrigerated. 

Hybrid TLC Plates 

Description Layer Qty. Part No. 

Silica Gel RP18/UV254s* Plates, Aluminum-Backed 

4 x 8cm 150µm 50 818144 

5 x 20cm 150µm 50 818145 

20 x 20cm 150µm 25 818146 

*Acid-resistant fluorescent indicator. 

Chiralplate † 

• Separates optically active isomers based on ligand 

exchange 

• Ready-to-use, requires no solvent pretreatment 

† 

Chiralplate was developed in cooperation with Degussa AG, Hanau, Germany. 

Chiralplate TLC Plates 

Description Layer Qty. Part No. 

Chiralplate, Glass-Backed 

5 x 20cm 250µm 50 811057 

10 x 20cm 250µm 4 811056 

10 x 20cm 250µm 25 811055 

20 x 20cm 250µm 25 811058 










Analtech TLC Plates 

Silica Gel H and HF Uniplates 

• Soft Layer 

– Contains no organic binder 

– Contains no calcium sulfate binder 

• Hard Layer 

– Contains an organic binder 

– 80% water resistant 

– Visualization by charring up to 150°C 

Silica Gel G and GF Uniplates 

• Calcium Sulfate Binder 

• Soft Layer 

– Contains no organic binder 

• Hard Layer 

– Contains an inorganic binder 

– 100% water resistant 

– Visualization with strong charring reagents 

– Abrasion resistant 

Silica Gel H and HF Uniplates 

Layer 

H 

HF* 

Description 

Thickness Qty. Part No. Part No. 

Soft Layer, No Binders 

Conventional 

20 x 20cm 250µm 25 710011 720011 

10 x 20cm 250µm 25 710021 7200210 

Scored, Four 5 x 20cm Sections 

20 x 20cm 250µm 25 710511 720511 

Preparative Uniplates 

20 x 20cm 500µm 25 710012 720012 

Hard Layer, Organic Binder 

Conventional 

20 x 20cm 250µm 25 746011 747011 

10 x 20cm 250µm 25 746021 747021 

Scored, Four 5 x 20cm Sections or Eight, 2.5 x 10cm Sections 

20 x 20cm 250µm 25 746511 747511 

10 x 20cm 250µm 25 746521 747521 

*F-Fluorescent Indicator, 254nm. 

HPTLC Uniplates 

• Smooth surface gives a high signal-to-noise ratio for 

increased sensitivity and precision 

• High resolution separations in five minutes 

• Ideal for densitometric scanning 

HPTLC Uniplates 

Description 

Layer 

Thickness Qty. Part No. 

HPTLC Silica Gel with Inorganic Binder 

10 x 10cm, HP-GHL 150µm 25 756077 

10 x 10cm, HP-GHLF* 150µm 25 757077 

HPTLC Unibond Amvvino (NH2) 

10 x 10cm, HP-NH2F 150µm 25 722077 

HPTLC Unibond Cyano (CN) 

10 x 10cm, HP-CNF 150µm 25 723077 

Reversed-Phase HPTLC Unibond 

10 x 10cm, HP-RP18 150µm 25 762077 

10 x 10cm, HP-RP18F 150µm 25 763077 


Silica Gel G Uniplates 

Layer 

Thickness 

G 

Part No. 

GF* 

Part No. 

Description 

Qty. 

Soft Layer, No Binders 

Conventional 

20 x 20cm 250µm 25 701011 702011 

10 x 20cm 250µm 25 701021 702021 

5 x 20cm 250µm 25 701031 702031 


20 x 20cm 250µm 25 701511 702511 

Preadsorbent 

20 x 20cm 250µm 25 731011 732011 

Preparative Uniplates, Soft Layer 

Conventional Prep 

20 x 20cm 500µm 25 701012 702012 

20 x 20cm 1000µm 25 701013 702013 

20 x 20cm 1500µm 25 701014 702014 

20 x 20cm 2000µm 25 701015 702015 

Preadsorbent Prep Silica Gel G 

20 x 20cm 500µm 25 731012 732012 

20 x 20cm 1000µm 25 731013 732013 

Hard Layer, Inorganic Binder 

Conventional 

20 x 20cm 250µm 25 711011 721011 

10 x 20cm 250µm 25 711021 721021 


20 x 20cm 250µm 25 711511 721511 



Looking for a fast and easy way to score . 

your own TLC plates? 

See our TLC plate cutter on page 196. 

3923 

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193


TLC Tanks and Storage 

Cylindrical TLC Tanks 

• Requires minimal solvent usage 

• Glass cylindrical tanks with press-on lids 

best 

seller 

Rectangular TLC Tanks 

• Unique beveled lip to 

eliminate sharp edges 

• Uniform flat top for maximum 

lid seal 

Cylindrical TLC Tanks 

Description 

Cylindrical TLC Tanks for 5 x 10mm and 4 x 8cm Plates, 

2.5" o.d., 5" Height 

Cylindrical TLC Tanks for 5 x 20cm Plates, 2.5" o.d., 

9" Height 

Cylindrical TLC Tanks for 10 x 20cm Plates, 5" o.d., 

9" Height 

Microslide TLC Tank 

• Small glass chamber holds up to 

10 microslide plates 

• Requires minimal solvent usage 

Part No. 

17108 

17107 

17002 

5341 

This Rectangular TLC Tank is 

a heavy wall, clear glass block 

with flat surface walls. Lids are 

polished glass in sizes to match 

the tank dimensions. 

Rectangular TLC Tanks 

Description 

Rectangular Tanks, All Supplied with Glass Lid 

Thinline Tank for 10 x 10cm Plates, 

(12cm x 11.5m x 6.4cm), (4.75" x 4.5" x 2.5") 

Standard Tank for 10 x 10cm Plates, 

(12cm x 11.5cm x 8.6cm), (4.75" x 4.5" x 3.37") 


(27cm x 24cm x 7cm), (10.5" x 10" x 3") 


(27cm x 13cm x 7cm), (10.5" x 5" x 3") 


(17cm x 15cm x 8cm), (7" x 6" x 3") 

Part No. 

7581 

7550 

7645 

7582 

7580 

5346 

tlc 

Microslide TLC Tank 

Description 

Part No. 

Microslide Tank 30020 

5342 

TLC Tank Pads 

• Medium weight lint-free pads for 

TLC tank liners 

Latch-Lid TLC Chromatotank 

• Unique latching device assures tight 

seal between lid and tank 

• No messy “grease” seals 

The unique latching device holds the 

matching ground glass surfaces of the 

lid and tank firmly in place to maintain an 

optimal equilibration atmosphere. The metal components 

of the Latch-Lid mechanism are made of stainless steel. 

Latch-Lid Chromatotank 

Description 

Part No. 

Standard Latch-Lid Tank/Lid Unit, 10 x 10cm Plates 7542 

Latch-Lid Tank/Lid Unit, 20 x 20cm Plates 7536 

5350 

TLC Pads 

Description 

Part No. 

20 x 20cm, Medium Weight, 100/pk 7630 

tech tip 

Rectangular TLC Tank Dimensions 

• All are inside dimensions 

• Listed as height x width x depth 

D 

H 

W 

5345 

0571 

Multiple Plate Development Rack 

• Saves time and money 

• Choice of anodized aluminum or PTFE 

resin-coated rack 

• Sizes available to hold six 20 x 20cm or 

six 10 x 10cm plates 


Need a timer? 

See page 265. 

6563 

5349 

Multiple Plate Development Rack 

Description 

Part No. 

Anodized Aluminum Rack for 20 x 20cm Plates 17051 

Anodized Aluminum Rack for 10 x 10cm Plates 17053 

PTFE-Coated Rack for 20 x 20cm Plates 17074 

PTFE-Coated Rack for 10 x 10cm Plates 17077 



Sprayers 

Compressed Gas Sprayer 

• Replaceable pressure unit 

• Refillable sprayer 

• Reusable glass or plastic 

container 

Compressed Gas Sprayers 

provide a constant pressure and 

uniform distribution of fine mist 

on your TLC plate. The pressure 

unit contains difluoroethane. 

Unit sprays up to 16oz of liquid. 

Caution: Wetted parts are made 

of polypropylene or nylon. Do not 

store with dip tube immersed in reagent. 

Safety Top Screw Cap Sprayer 

• Made of borosilicate glass 

• Autoclavable 

Safety Top Sprayers adjust 

the mist by covering the rear 

vent hole with a thumb. The 

atomizer tops are attached to 

the reservoir flasks with a screw 

top and o-ring. The screw top 

delivers a positive seal between 

sprayer head and reservoir, 

eliminating the possibility 

of “blow back”. Remove the 

screw cap and o-ring prior to 

autoclaving. Operation of the 

unit is by low air pressure (5lb). 

best 

seller 

Compressed Gas Sprayer 


Complete Spray Unit with Glass Jar 3 14654 

Replacement Glass Jar with Lid 6 14655 

Disposable Poly Jar with Lid 6 14656 

Replacement Compressed Gas Unit 6 14657 

5329 

5332 

Atomizer Sprayer 

• Atomizer bulb or 

compressed gas 

propellant source 

This borosilicate glass 

sprayer has a rear vent hole 

that allows full control of 

mist. It has a 4oz (125mL) 

reservoir. It is suitable for use 

with most corrosive liquids. 

Atomizer Reagent Sprayer 


Atomizer Reagent Sprayer ea 17017 

Spraying Accessories 

Disposable Spray Box 

• Opens easily into 14"L x 14"W x 14"H 

(35.56cm) unit 

• Unique design for spill containment 

• Heavy duty cardboard with disposal 

bag provided 

Stainless Steel Spray Support 

• Self-draining 

• Holds plate above the 

spraying surface 

5331 

5367 

5369 5407 

tlc 

Safety Top Sprayer with Screw Cap 


10mL ea 14540 

50mL ea 14545 

125mL ea 14550 

250mL ea 14555 

Polypropylene Spray Stand 

• Front edge to hold plate for spraying 

• Ideal for use in fume hood 

TLC Spray Supports 


Disposable Spray Box 5 16408 

Stainless Steel Spray Support ea 16409 

Polypropylene Spray Stand ea 7636 

5370 


195


TLC Accessories 

TLC Plate Cutter 

• Scores coated glass plates at 

any position 

• Compact, portable, and easy 

to operate 

The TLC Plate Cutter has a highquality 

carbide scriber mounted 

into a movable plastic head. It is designed to produce optimal 

scoring on the glass surface of 20 x 20cm TLC plates with a 

total thickness (glass + coating) of 2mm. A smaller template 

is included with the unit for ease in cutting 10 x 10cm plates. 

3923 

TLC Spotting Guide 

This template fits over a standard 20 x 20cm 

TLC plate. 1cm divisions allow for accurate 

sample application. After development, 

a series of labelled circles and lines on 

the template helps to estimate areas or 

to measure distances for Rƒ calculations. 

best 

seller 

Spotting Accessories 


TLC Spotting Guide ea 7624 

4128 

tlc 

TLC Plate Cutter 


TLC Plate Cutter ea 7535 

Replacement Scribers 1 7565 

3 7575 

tech tip 

Note About Sample Applications 

Thorough drying of samples is a step that is often ignored but can 

result in development errors. If a small amount of solute is left on 

the plate it can interact with the development system and affect 

the separation. 

Many analysts assume the plate is dry when the solvent is no 

longer visible on the plate (5–10 minutes). However, depending 

on the volatility of the solvent and the quantity of sample applied, 

the drying time can be considerably longer. Generally, Rƒ values 

decrease as drying time increases until the dry state (stable Rƒ) 

is reached. 

Plates can absorb moisture from the atmosphere during storage, 

so prior to sample application, all TLC plates should be heated 

at 120–130°C for about 30–40 minutes and allowed to cool in 

a desiccator. This ensures reproducible Rƒ values. 

Universal Spotting Template 

• Use to cut channels or to spot plates 

• Measure Rƒ values 

• Non-slip surface works with any size 

TLC plates 



Universal Spotting Template ea 26270 

TLC Plate Coating Supplies 

• Desaga plate coating device 

and template 

• Adsorbosil ® -Plus powders and 

polished glass plates 

Plate Coating Supplies 


Desaga Plate Spreading Device and Template 

Plate Spreading Device ea 95116 

Plate Spreading Template ea 95118 

Polished Glass Plates 

20 x 20cm Plate, 3mm Thick 10 26080 

Alltech ® Adsorbosil ® Powders 

Adsorbosil ® -Plus 1 1lb 160201 

Macherey-Nagel Powders 

Cellulose MN 301 1000g 81605 

4130 

5384 

TLC Plate Scriber 

• Cuts channels in soft and hard layers 

• Adjust channel widths in multiples 

of 1cm 

• Made of flexible stainless steel 



TLC Plate Scriber ea 17102 

4132 




Sample Applicators 

Drummond ® Microcaps 

• Accuracy of ±1% 

The Drummond ® Microcap is a 

precision bore glass capillary tube 

that holds a known volume when 

filled by capillary action. The tube is 

emptied completely by squeezing 

the dispenser bulb. (Bulb is included with the unit.) 

Drummond ® Sample Applicators 

Description/Capacity Qty. Part No. 

Drummond ® Microcaps 

0.5µL 100 3808 

1.0µL 100 3809 

2.0µL 100 3810 

3.0µL 100 3811 

5.0µL 100 3814 

10.0µL 100 3820 

15.0µL 100 3821 

20.0µL 100 3822 

25.0µL 100 3823 

50.0µL 100 3828 

100.0µL 100 3838 

Drummond ® Microcap Accessories 

Microcap Bulbs 6 3940 

5338 

Drummond ® Wiretrol ® Micropipettes 

• Combines the accuracy of a 

microsyringe with the ease and 

economy of a disposable pipette 

• Accuracy of ±1% 

• Color-coded with a permanent 

calibration line 

These unique pipetting devices 

feature one-handed operation. 

Each unit contains a vial of 100 

micropipettes, dispenser cap, handle, 

and one stainless steel plunger. 

Drummond ® Sample Applicators 


Drummond ® Wiretrol ® Micropipettes 

1.0 to 5.0µL (Calibrated) 100 3842 

3.0µL 100 3843 

5.0µL 100 3844 

10.0µL 100 3845 

20.0µL 100 3846 

25.0µL 100 3847 

50.0µL 100 3849 

100.0µL 100 3851 

5286 

Uncalibrated Disposable Micropipettes 

Uncalibrated disposable glass 

micropipettes are intended for rapid 

qualitative sample application on 

TLC plates. Thick walls make these 

pipettes strong and easy to handle. 

Overall length is about three inches. 

Capacity is approximately 2–8µL. 

Uncalibrated Disposable Micropipettes 


Glass Micropipettes 300 7616 

1440 17203 





Syringes for Thin Layer Chromatography 

• PTFE-coated needle tip (3/4") 

• Standard 2" needle length 

5340 5283 

TLC Syringes 


TLC Syringes with PTFE-Coated Needles 

Fixed Needle, Blunt Point 

10µL, 26s ga. ea 800500 

25µL, 22s ga. ea 802500 

50µL, 22 ga. ea 80950 

100µL, 22 ga. ea 81050 

tlc 


197



UV Viewing Cabinet 

• Fixed eyepiece with UV 

shielded window 

• Uses 4- and 6-watt E-series 

lamps 

TLC Sample Recovery Tubes 

• Removes samples from TLC 

plates by suction 

• Reusable, all glass 

construction 

The UV Viewing Cabinet measures 

6.5" H x 9" W x 12" L (16.5cm H x 

22.9cm W x 30.5cm L). This cabinet 

cannot be used for photo systems 

because of the fixed eyepiece. 

UV Viewing Cabinet, Supplied Without Lamps 

Description 

Part No. 

UV Viewing Cabinet, Model CM-10 80305 

5352 

Connect a vacuum to one end of 

the sample recovery tube and pull the sample into the 

tube. Then pass solvent through the recovery tube to extract 

the sample. 

TLC Sample Recovery Tubes 


0.5–1mL Sample Recovery Tube ea 17010 

1–2mL Sample Recovery Tube ea 17011 

25–30mL Sample Recovery Tube ea 17012 

5288 

tlc 

E-Series Ultraviolet Lamps 

• Easy to operate 

• Choice of intensity, wavelengths, 

and sizes 

The Spectroline ® E-Series Lamps can be 

used as portable units, with UV viewing 

cabinets, or in photo darkroom cabinets. 

Lamps with a filter assembly provide 

maximum fluorescent contrast with the 

least white light transmission. 

E-Series Lamps 

Description 

Part No. 

8-Watt Combo Lamp 

LW, SW, and Filter Assembly, 115V 80240 


8-Watt Replacement Parts 

LW Replacement Tube 80242 

SW Replacement Tube 80244 

Combo Light Replacement Filter 80246 





LW Tube Replacement 80252 

SW Tube Replacement 80254 

Combo Light Filter Replacement 80256 





LW Tube Replacement 80272 

SW Tube Replacement 80274 

Combo Light Filter Replacement 80276 

Lamp Accessories 

UV Lamp Stand 80218 

UV Lamp Handle (not shown) 80219 

5353 

pH-Fix Universal Indicator Sticks 

• Four indicator papers 

(6 x 5mm) are sealed to each 

6 x 85mm stick 

pH-Fix Universal Indicator Sticks 


Range 0–14, 1.0pH Units 100 18147 

Range 4.5–10, 0.5pH Units 100 18158 

Range 7.0–14, 0.5pH Units 100 18160 

Adsorbent Scraper 

• Blade mounted in “easy-grip” handle 

13mm—Scrape Large Areas 

6mm—Small Spots, Ideal for Radial Chromatography and . 

Linear HPTLC 

2mm—Score Lines, Channels, and Scrape Small Spots 

TLC Scrapers 


TLC Scraper Kit: 1 of each type Kit 7626 

13mm TLC Scraper ea 7627 

Replacement Blades, 13mm 10 7627B 





0614 

5366 


GC 

GC Instruments. . . . . . . . . . . . . . . . . 200–212 

GC Systems & Data Collection . . . . . . . . . . . 200–205 

Gas Generators 

Hydrogen. . . . . . . . . . . . . . . . . . . . . . . . . 206–207 

Air . . . . . . . . . . . . . . . . . . . . . . . . . . . . 208–209 

Nitrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210 

Air Compressors . . . . . . . . . . . . . . . . . . . . 211–212 

GC Columns. . . . . . . . . . . . . . . . . . 213–258 

Introduction. . . . . . . . . . . . . . . . . . . . . . 213–214 

Alltech ® Capillary Columns. . . . . . . . . . . . . . 215–234 

Custom Columns . . . . . . . . . . . . . . . . . . . . . . . 215 

Alltech ® Heliflex ® and Alltech ® Econo-Cap . . . . . . 216–231 

EPA Methods Guide. . . . . . . . . . . . . . . . . . . 232–234 

Distributed Capillary Columns . . . . . . . . . . . . 235–238 

Astec . . . . . . . . . . . . . . . . . . . . . . . . . . 235–236 

SGE ® . . . . . . . . . . . . . . . . . . . . . . . . . . . 237–238 

Alltech ® Packed Columns . . . . . . . . . . . . . . 239–258 

Custom Packed . . . . . . . . . . . . . . . . . . . . . 239–243 

Popular Packed . . . . . . . . . . . . . . . . . . . . . 244–245 

CTR Concentric Packed Columns . . . . . . . . . . . . . 246 

Pretested Packings . . . . . . . . . . . . . . . . . . . 247–248 

Custom Coated Packings . . . . . . . . . . . . . . . 249–250 

GC Supports and Packings . . . . . . . . . . . . . . 251–258 

GC Accessories . .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .259–294 

Capillary Accessories . . . . . . . . . . . . . . . . 259–261 

Packing Accessories . . . . . . . . . . . . . . . . . 262–263 

Gas Flow and Detection . . . . . . . . . . . . . . . 264–267 

Gas Purification . . . . . . . . . . . . . . . . . . . 268–275 

Gas Sampling . . . . . . . . . . . . . . . . . . . . 276–279 

Ferrules. . . . . . . . . . . . . . . . . . . . . . . . 280–281 

Fittings . . . . . . . . . . . . . . . . . . . . . . . . 282–285 

Injection Liners & Accessories . . . . . . . . . . . . 286–288 

Septa . . . . . . . . . . . . . . . . . . . . . . . . . 289–292 

Valves . . . . . . . . . . . . . . . . . . . . . . . . 293–294

gas chromatography 

SRI Gas Chromatographs 

• Value-priced — Typically half the price of comparable 

units from other manufacturers 

• Versatile — Choose from a large selection of GC 

detectors and injectors 

• PC Programmable — PeakSimple data control 

software included 

Choose from Two Chassis: 

Model 8610C 

– for performance and maximum flexibility 

Model 310 

– for economy and compact size 

BioDiesel GC System 

SRI BioDiesel GC Systems are configured to perform ASTM 

6584. Analysis takes about 25 minutes and is simple enough 

to run in the field. 

Systems Include: 

• Choice of 8610C or 310 chassis 

• FID detector 

• On-column injector 

• 15-meter wide-bore capillary column 

• PeakSimple software 

gc instruments 

2342 3893 

Model 8610C Model 310 

SRI GC Specifications 

SRI GC Model: 8610C 310 

Temperature: Ambient to 400ºC Ambient to 400ºC 

Programmable: Yes, Multiple Steps Yes, Multiple Steps 

Oven Inches: 3" H x 7.5" W x 7.5" D 3" H x 5" W x 5" D 

Oven Metric: 7.6cm x 19.1cm x 19.1cm 7.6cm x 12.7cm x 12.7cm 

Capillary Column: 7" Cage Capillaries 4" Cage Capillaries 

Packed Column: 1/4" or 1/8" 1/8" 

Detectors: Up to 4 Up to 4 

Inlets: On-Column, Up To 5 On-Column, 1 inlet 

Dimensions Inches: 13" H x 18.5" W x 14.5" D 13" H x 12.5" W x 14.5" D 

Dimensions Metric: 33cm x 47cm x 36.8cm 33cm x 31.7cm x 36.8cm 

Weight: 45–80lb (20–36kg) 40–70lb (18–32kg) 

more info 

SRI Systems are CE, TUV, GS, and NRTL approved. SRI 

manufactures all instruments in compliance with EN 61010 

standards for laboratory equipment. Instruments come with 

a two-year warranty. 

BioDiesel GC Systems 

7380 

110V 

Part No. 

220V 

Part No. 

Description 

BioDiesel Analyzer on 8610C Chassis 8618932 8618933 

BioDiesel Analyzer on 310 Chassis 8618934 8618935 

Every SRI GC Comes Complete with: 

• Single on-column injector for 0.53mm i.d. capillary or 

1/8" packed columns or injectors of choice 

• Electronic pressure control for all gases 

• Temperature programmable oven—programmable in 

multiple segments 

• Digital pressure readout 

• Digital temperature readout—ambient to 400°C 

• Built-in data system with PeakSimple for Windows ® 

software 

• Operator’s manual 

• 10μL syringe and spare septa 

• Heavy-duty reusable shipping container. Shipped 

easily by common carriers (UPS or FedEx) 

7381 


Looking for more biodiesel analysis solutions? 

Visit www.gracebiofuels.com. 



Preconfigured SRI GCs 

Enjoy the Convenience of Ordering a System 

Already Optimized for Your Application 

Preconfigured GCs are designed with inlet systems, 

detectors, and accessories required for a specific application. 

The preconfigured GCs are also supplied with an internal data 

acquisition system and PeakSimple for Windows ® software 

to control the GC and acquire and analyze the data. 

Educational GC – 310 Chassis 

Gasless with CCD (built-in air compressor) 

• Standard on-column injector 

• Detects combustible (hydrocarbon) molecules 

• Packed GC column included 

Multiple Gas Analyzer—8610C Chassis 

Separates H2, O2, N2, CH4, CO2, and Hydrocarbons. 

Model 1—Fixed Gases and C1 to C6 Hydrocarbons 

• Two columns—MoleSieve 13x and silica gel 

• Sensitivity for CO and CO2 to ppm level 

• 10-port gas sampling automatic valve and loop 

Model 1—Multiple Gas Analyzer 

110V 

Part No. 

220V 

Part No. 

Description 

Model 1 with TCD Single Channel 8610022 8610512 

Model 1 with TCD/FID* 8610023 8610513 

Model 1 with TCD/HID* 8610501 8610514 

*Four-channel PeakSimple data system. 

Model 310 with CCD 

110V 

Part No. 

220V 

Part No. 

Description 

Gasless with CCD with cooling 31001105 310502 

Gasless with CCD without cooling 8611252 8611305 

TCD System (fast and manual cool down) 

• Universal detector sensitive from 100% to 200ppm 

• Can analyze room air, human breath 


Model 310 with TCD 

Description 

110V 

Part No. 

220V 

Part No. 

Model 310 with TCD 8610001 310501 

Models 1 and 2 Plus Sulfur 

Multiple gas and sulfur analysis in one unit. Detects low sulfur 

concentrations. 

• TCD, FID-methanizer and FPD/FID detectors 

• Uses just one gas sampling valve with Silcosteel ® 

• 2-meter MoleSieve and silica gel columns 

• 60-meter capillary column 

• Built-in air compressor 

• Four-channel PeakSimple data system 

Models 1 and 2 Plus Sulfur—Multiple Gas Analyzer 

Description 

110V 

Part No. 

220V 

Part No. 

Model 1 Plus Sulfur 8611271 8611325 

Model 2 Plus Sulfur 8611274 8611307 


FID System 

• Carrier and combustion gas 


Model 310 with FID 

Description 

110V 

Part No. 

220V 

Part No. 

Model 310 FID with cooling 8611251 8611306 

Basic Capillary FID GC—8610C Chassis 

• Hydrocarbon selective from 100% to 1ppm 


• 30-meter x 0.53mm capillary column included 

Basic Capillary FID GC 

Description 

110V 

Part No. 

220V 

Part No. 

Basic Capillary FID GC 8610523 8610524 

Model 2 Fixed Gases and C1 to C20 Hydrocarbons 

• Heated 10-port gas sampling valve (electrically actuated) 

• Column switching 

• Also measures water and alcohol 

• Two packed GC columns 

Model 2—Multiple Gas Analyzer 

110V 

Part No. 

220V 

Part No. 

Description 

Model 2 with TCD 8610503 8612232 

Model 2 with TCD/FID/Methanizer 8610504 8612233 

Model 2 with TCD/HID 8611273 8610514 

Sulfur GC—8610C Chassis 

For the analysis of H2S in natural gas, total reduced sulfur 

analysis, mercaptans, and sulfur containing pesticides. 

• FID/FPD combination detector 


• 1-meter PTFE Hayesep ® D column 

• 10-port gas sampling valve with nickel loop 


Sulfur GC 

Description 

110V 

Part No. 

220V 

Part No. 

Sulfur GC 8610525 8610517 


201



Preconfigured SRI GCs 

BTU Gas Analyzer—8610C Chassis 

For the analysis of natural gas and computing the BTU rating. 

Measures nitrogen, methane, carbon dioxide, ethane, water, 

propane, iso and normal butanes, iso and normal pentanes, 

and C6 plus hydrocarbons. 

• Thermal conductivity detector 

• 10-port gas sampling valve 

• Packed GC column 

• 30 meter capillary column 

BTU Gas Analyzer 

Description 

110V 

Part No. 

220V 

Part No. 

BTU Gas Analyzer 86103070 8610518 

Environmental GC—8610C Chassis 

Ideal for laboratory or field-testing applications where space, 

price, and versatility are crucial. The Environmental GC is 

designed for EPA methods 5030, 5030/5035. It will easily 

generate certification quality data for EPA Methods 8021, 

8010, 8015, TO-14, and many others. 

• FID, PID, and DELCD detectors 

• Equipped with the method 5030/5035 compliant purge 

and trap 




Environmental GC 

Description 

110V 

Part No. 

220V 

Part No. 

Environmental GC 8610058 8612258 

PCB GC—8610C Chassis 

Detects PCBs in soil and other solids. Also used for analysis 

of pesticides, PAHs, JP-4, kerosene, and diesel. Accepts 

1-gram samples. 

• FID/DELCD combination detector 

• Built-in thermal desorber 



PCB GC 

Description 

110V 

Part No. 

220V 

Part No. 

PCB GC 8611265 8611308 

BTEX GC—8610C Chassis with FID/PID 

Designed for the analysis of benzene, toluene, ethylbenzene, 

and xylenes. 

• Method 5030/5035 compliant purge and trap 




BTEX GC 

110V 

Part No. 

220V 

Part No. 

Description 

BTEX GC 8612250 8610510 

5030/5035 Compliant Upgrade 8611267 8611309 

TO-14 GC—8610C Chassis 

Designed for the TO-14 or an ambient air analysis. 

• PID and FID/DELCD detectors 

• Dual trap sample concentrator 


• Vacuum pump interface 



TO-14 GC 

Description 

110V 

Part No. 

220V 

Part No. 

TO-14 GC 8610502 8610515 

Mud-Logging GC—8610C Chassis 

Reports the total hydrocarbons of a gas stream as well as 

performing separations. 

• Flame ionization and catalytic combustion detector 

• Packed GC column 


• 10-port gas sampling valve 


Mud-Logging GC 

Description 

110V 

Part No. 

220V 

Part No. 

Mud-Logging GC 8610500 8610511 

TOGA GC System—8610C Chassis 

This portable unit determines the gases dissolved in 

transformer oil. 

• HID and/or TCD detectors 

• MoleSieve and silica gel-packed columns 

• Syringe pump for industry standard 50mL syringes 

• Dual 10-port gas sampling valves 

• Gas extraction loop 

TOGA GC System 

110V 

Part No. 

220V 

Part No. 

Description 

TOGA with TCD Detector 8611326 8611327 

TOGA with HID Detector 8611328 8611329 

TOGA with TCD and HID Detector 8611330 8611331 



SRI Accessories 

Method 5035 Compliant Purge and Trap 

Ideal for higher level soil samples or 

soil/methanol extractions. Uses low 

cost disposable 16mm test tubes which 

are purged at ambient temperature. 

Method 5035 Compliant Purge and Trap 

Description 

Part No. 

Method 5035 Compliant Purge and Trap 8690051 

Upgrade Purge and Trap 5030/5035 Compliant 8611302 

SRI 10-Port Gas Sampling Valve 

Factory plumbed and tested according to your application. 

Can only be mounted on the 8610C GC. 

SRI 10-Port Gas Sampling Valve 

Description 

Part No. 

10-Port Gas Sampling Valve—Manual 8690063 

10-Port Gas Sampling Valve—Electric 8690065 

Thermostatted Valve Oven 

Can only be mounted on the Model 8610C GC. 

Accommodates one or two electronically actuated and one 

manually actuated valve. The oven can be heated to 175°C 

for electrically actuated valves and autosamplers. 

6685 

Custom SRI Systems 

Follow these basic instructions to configure your custom GC. 

On either chassis, you can order as many as four detectors. 

On the Model 310 chassis, you are limited to one sample inlet 

system; on the Model 8610C you may order as many as five. 

If you need a gas sampling valve, you must order a Model 

8610C. If you have any questions, contact our Technical 

Support group. 

How to Build a Custom GC: 

1. Choose a chassis with the appropriate channels. 

(page 203) 

2. Choose inlet system; as many as five for 8610C, 

one for 310. (page 204) 

3. Choose up to four detectors. (page 204) 

SRI Chassis 

Model 310 Chassis 

110V 

Part No. 

220V 

Part No. 

Description 

With 1-Channel PeakSimple 8611249 8611250 



3893 


Thermostatted Valve Oven 

Description 

Part No. 

Thermostatted Valve Oven 8690088 

Model 311H 42 Vial Liquid Autosampler 

Injection volume is adjustable from 0–3µL. 

Requires 1mL of sample and an inert gas 

source at 60psig to operate. One hundred 

screw cap vials and septa are included. 

Model 8610C Chassis 

110V 

Part No. 

220V 

Part No. 

Description 




2342 

Model 311H 42 Vial Liquid Autosampler 

Description 

Part No. 

Model 311H 42 Vial Liquid Autosampler 8690068 

7399 


203



SRI Inlet Systems 

Every SRI GC is equipped with an unheated on-column inlet 

system. On a Model 310 chassis, you can replace that with 

any one of the other four inlet systems available—headspace, 

split/splitless, purge and trap, and flash. On a Model 8610C 

chassis, you can mount any of the inlet systems to a total 

of five. 

On-Column Inlet Systems 

Suitable for 0.53mm capillary and 1/8" packed columns. 

Includes EPC carrier gas controller and molecular sieve filter. 

On-Column Inlet Systems 

Description 

Part No. 

Additional On-Column Injector 8690023 

Heated Static Headspace Inlet System 

Ideal for the analysis of volatiles, especially where the sample 

matrix is dirty. Sample is heated and injected via a sample 

loop and gas sampling valve. Vial sleeve can be heated to 

90°C. Requires 10–20mL of sample for operation. 

Heated Static Headspace Inlet Systems 

Description 

Part No. 

Heated Static Headspace Injector 8690045 

SRI Detectors 

The SRI GC allows you to choose the detector(s) you want 

installed. The chassis can accommodate as many as four 

detectors to give you maximum flexibility. 

Table 1 – Detector Specifications 

Detector Selectivity Sensitivity (Approx.) 

CCD Hydrogen and hydrocarbons 500ppm 

TCD Universal 200-500ppm 

FID Hydrocarbons 1ppm 

DELCD Chlorinated and brominated 10ppb 

molecules 

FID/DELCD Hydrocarbons, chlorinated and 10ppm 

brominated molecules 

HID Universal with exception of neon 10ppm 

PID 

Aromatics and molecules with double 10ppb 

carbon bonds 

NPD Nitrogen and phosphorus 10ppm 

NPD/DELCD Nitrogen, phosphorus, chlorinated 100ppb 

and brominated 

TID Nitro functional groups (TNT etc.) 1ppb, 50ppb 

ECD Electronegative compounds 10ppb 

RGD Reducing gases (such as H2 and CO) 10ppb 

FPD Sulfur and phosphorus 200ppb and 10ppb 

FPD/FID Sulfur, phosphorus, and 

hydrocarbons 

200ppb, 10ppb, and 

100ppm 

Dual FPD Sulfur and phosphorus 

simultaneously 

200ppb and 10ppb 

FID Dual FPD 

Hydrocarbons, sulfur, phosphorus 

simultaneously 

100ppm, 200ppb, 

and 10ppb 

Heated Split/Splitless Injector 

Permits the use of narrow-bore capillary columns (0.32mm i.d. 

and smaller) in either split or splitless modes. Allows 0.53mm 

capillary columns and 1/8" packed columns to be used in 

split, splitless or on-column modes. Injector temperature is 

adjustable or programmable to 300°C. 

Heated Split/Splitless Injector 

Description 

Part No. 

Heated Split/Splitless Injector 8690034 

Heated Split/Splitless Injector with PTV 8611303 

Heated Flash Vaporization Injector 

Vaporizes the sample prior to the column. Ideal for desorption 

of SPME fibers or injection of extremely dirty samples. The 

injector heater block can be heated to 300°C. 

Heated Flash Vaporization Injector 

Description 

Part No. 

Heated Flash Vaporization Injector 8690025 

SRI GC Detectors (choose up to four per chassis) 

Description 

Part No. 

CCD (Catalytic Combustion Detector) 86902007 

TCD (Thermal Conductivity) 8690107 

FID (Flame Ionization) 8690010 

DELCD (Dry Electrolytic Conductivity) 86901026 

FID/DELCD Simultaneous 8692026 

HID (Helium Ionization Detector) 8690030 

PID (Photo-Ionization) 8690040 

NPD (Nitrogen-Phosphorus) 8690015 

NPD/DELCD (Series Detector) 8611299 

TID (Thermionic Ionization Detector) 8690017 

ECD (Electron Capture) 8690020 

RGD (Reduction Gas Detector) 8611293 

FPD (Flame Photometric) 8690080 

FPD/FID Simultaneous 8691080 

DFPD (Dual Flame Photometric) 8690085 


Need septa? 




PeakSimple for Windows ® 

• Included with every SRI gas chromatograph 

• Runs on Windows ® 95/98/Me or 

Windows ® NT/2000/XP 

• Interfaces through available serial port or USB 

PeakSimple software is simple, fast, and powerful. 

Each SRI GC comes with a built in data system and the 

PeakSimple software is the interface between your GC 

and PC. The PeakSimple software will run on any PC with 

a Windows ® 95/98/Me or Windows ® NT/2000/XP operating 

system. The software communicates to the GC through an 

available serial port or USB. 

Features: 

• Unlimited temperature and event entries—user defined 

temperature and event programs have no size or entry 

limitations, allowing a “fine-tune” for each run. 

• Multiple point calibration curves—permits exact 

quantification of sample components in analysis reports. 

• Manual and automated integration correction— 

manipulate baseline and integration during and after run. 

• Peak grouping—for PCB’s and TPH analysis. 

• ASCII or binary file storage—for export to 

spreadsheets, etc. 

• Automatic calibration at seven concentration levels 

with six curve fitting options including straight line, 

multi-line, parabolic, and quadratic. Choice of area 

or height calculation for each peak. 

• High/Low peak alarms with link to user programs if 

alarm levels are exceeded. 

• Baseline subtraction, peak smoothing, valley 

deconvolution, peak reverse, drift correction. 

• Overlay chromatograms for easy visual comparisons. 

• Autosampler queues for unattended operation allows 

mixed methods, auto-recalibration. 

• Batch reprocessing of previously run chromatograms. 


PeakSimple for Windows ® gives you the flexibility and power to 

meet all your chromatographic needs. 

4541 

Also Available: 

Stand Alone Serial Data System 

• Comes with PeakSimple for Windows ® software 

• Single-, four-, or six-channel models 

6690 

Stand Alone Serial Data System 

110V 

Part No. 

220V 

Part No. 

Description 

With 1-Channel Serial Port 8612010 86001255 

With 4-Channel Serial Port 86002055 86022055 

With 6-Channel USB 86006055 86006255 


Looking for other data systems? 


PeakSimple provides for seven calibration points and six different 

types of calibration; from linear to quadratic. 

4542 


205


Hydrogen Generators for GC Applications 

High-Purity Hydrogen from Parker Balston ® 

and Domnick Hunter ® 

• Provide a safe, reliable source of hydrogen with purity 

to 99.99999+% 

• Eliminate the cost and inconvenience of dealing with 

hydrogen and helium cylinders 

• Promote compliance with OSHA and NPFA guidelines 

for indoor hydrogen use 

• Includes a wide range of flows to meet your needs 

Parker Balston ® and Domnick Hunter ® are now one company 

offering exceptional gas generation technologies from a single 

source. Both brands offer different technologies and several 

flow rates to accommodate either single or multiple GC 

applications. 

Parker Balston ® . 

Hydrogen Generator 

7436 

Domnick Hunter ® . 


7396 


GC instrumentation uses hydrogen as either a fuel gas 

(FID, FPD, and NPD) or carrier gas. Each application requires 

specific and unique gas purity. Parker Balston ® offers both a 

desiccant cartridge caustic-free system for fuel applications 

and a palladium-based carrier gas range providing the purest 

hydrogen commercially available. Domnick Hunter ® offers a 

caustic free, carrier grade generator with a durable, built in 

micro-dryer that eliminates the need for regular desiccant 

cartridge replacement. 

Parker Balston ® Hydrogen Generator Technology 

Pressure Gauge 

Water/Gas 

Separators 

Shutoff 

Valve 

Water 

Supply 

Scrubbing 

and Drying 

Hydrogen 

Outlet 

Pressure 

Transducer 

Check 

Valve 

Relief 

Port 

Drain 

Port 

Proton Exchange 

Membrane Cell 

7452 



Hydrogen Generators for GC Applications 

Hydrogen Generators Specifications 

Parker Balston 

Domnick Hunter 

Model H2PEM-100 H2PEM-165 H2PEM-260 H2PEM-510 H2-150 H2-300 20H-MD 40H-MD 60H-MD 

Flow (cc/min): 100 165 260 510 150 300 160 250 500 

Use: Fuel Gas Fuel and Carrier Fuel and Carrier 

Max. Number of FIDs: 2 4 6 12 3 6 4 6 12 

Max. Number of Injectors/FIDs: N/A 1 2 1 2 4 

Purity: 99.9995% 99.99999% 99.9999% 

Water Quality: DI Water NaOH Solution DI Water 

Purifier Type: Dessicant Palladium Dessicant 

Regenerative Purifier: No No Yes 

Maximum Pressure (psig): 100 60 100 

Dimensions (h x w x d): 17.1" x 13.5" x 17.9" 

(43.5cm x 34.2cm x 45.6cm) 

22" x 12" x 13" 

(58cm x 30cm x 33cm) 

18" x 13.5" x 17.2" 

(45.6cm x 13.5cm x 43.7cm) 

Weight: 40lb (18kg) 58lb (26kg) 62lb (28kg) 

Hydrogen Generators 

Description 

Part No. 

Parker Balston ® 

Parker Balston ® H2PEM-100, 110/220V 8619101 




Parker Balston ® H2-150, 110V 80109 




Domnick Hunter ® 

Domnick Hunter ® 20H-MD, 110/220V 8618711 




Parker HydroGen Mate ® DI Water System 

Economically Provide DI Water to Your 


• Removes organics, phosphates, chlorine, and most 

ionizable constituents 

• Quick and easy installation 

• Easy-fill dispensing nozzle 

• No electrical requirements 

The Parker HydroGen Mate ® DI Water System provides 

high purity deionized water to all types of Parker hydrogen 

generators. The system is ready to install and includes 

prefiltration, two DI resin exchange cartridges, dispensing 

nozzle, and a final filter. 

Parker HydroGen Mate ® DI Water System 

Description 

Part No. 

Complete DI Water System 8619043 

Maintenance Kit* 8619044 

*Includes two replacement cartridges and one replacement final filter. 

7437 


207


Parker Balston ® FID Gas Station 

Hydrogen and Zero Air from a . 

Single Generator 

• Safer and more convenient than gas cylinders 

• Save bench space with a single unit 

• Minimal operator attention required 

• Quiet operation 

The Parker Balston ® FID Gas Station combines Parker’s zero 

air and hydrogen generation technologies into a single unit, 

eliminating the inconveniences and cost of hydrogen and 

gas cylinders. The Model FID-1000 gas station will supply 

90cc/min hydrogen and 1000cc/min of zero grade air and 

can supply fuel gas for two flame ionization detectors. With 

a 250cc/min hydrogen and 2500cc/min zero air generating 

capacity, the FID-2500 gas station can supply gases for up 

to six flame ionization detectors. 


Hydrogen Generation 

Hydrogen gas is produced from deionized water using a 

Proton exchange membrane cell. The hydrogen supplied 

is 99.9995% pure with pressures up to 60psig. 

Zero Air Generation 

The FID Gas station improves FID baseline stability by 

providing zero grade air from an on-site compressed air 

source. The FID-1000 produces zero air containing


Zero Air Generators 

Improve your Productivity by Using. 

Air Generators Instead of Cylinders 

• Replace bulky cylinders that need frequent changing 

• Consistently high-purity for high-quality GC results 

Gas Chromatography requires zero air or air


UHP Nitrogen Generators 

Ideal for GC and for TOC 

Gas Chromatography applications require ultra high purity 

nitrogen. With GC, nitrogen may be a carrier gas or makeup 

gas but regardless it needs low ppm oxygen, carbon 

dioxide, carbon monoxide, and hydrocarbon. These low levels 

of impurities are needed to protect the column and some 

detectors. 

Total Organic Carbon (TOC) analyzers use special purified 

nitrogen as a purge gas. It is important that both the air and 

nitrogen be hydrocarbon (including Methane), CO2 and CO 

free. This allows for the best signal to noise ratio with the TOC 

analyzer. The TOC Nitrogen Gas Generator is zero grade 

(


JUN-AIR ® Oil-Less Air Compressors 

Standard—B Series 

Complete compressed air unit with a 

holding tank. 

Standard Air Compressors* 

Description 

Part No. 

Model OF302-4B 89299 

Model OF302-25B 89279 

Model 2000-40B 89319 

Model 4000-40B 89272 

*Must specify voltage when ordering. Choose from 120V/60Hz, 240/60Hz, or 

230V/50Hz. Two-year manufacturer’s warranty. 

Standard Plus Air Dryer—D Series 

The D series is equipped with filters that 

have manual and automatic drains and an 

adsorption dryer to ensure 100% clean, 

dry, and oil-free air. 

Air Compressors with Dryer Option* 

Description 

Part No. 

Model OF302-4BD2 89376 

Model OF302-25BD2 89239 

Model 2000-40BD2 89328 

Model 4000-40BD3 89273 



6705 

6704 


Tank 

Size 

L/min 

Flow at 

80psig 

L/min 

Flow 

at 

60psig 

L/min 

Noise 

db(A) 

Approx.. 

H x W x L 

Compressor 

Standard 

OF302-4B 4 57 60 66 13 x 12 x 15" 

(33 x 30 x 38cm) 

Weight. 

lb (kg) 

49 (31) 

OF302-25B 25 57 60 66 23 x 15 x 15" 62 (39) 

(58 x 38 x 38cm) 

2000-40B 40 120 130 72 25 x 21 x 17" 72 (45) 

(64 x 53 x 43cm) 

4000-40B 40 250 275 75 26 x 24 x 18" 185 (116) 

(66 x 61 x 46cm) 

w/Air Dryer 

OF302-4BD2 4 46 48 66 14 x 15 x 20" 59 (37) 

(36 x 38 x 51cm) 

OF302-25BD2 25 46 48 48 23 x 20 x 18" 73 (46) 

(58 x 51 x 46cm) 

2000-40BD2 40 96 104 72 25 x 20 x 23" 73 (46) 

(64 x 51 x 58cm) 

4000-40BD3 40 200 220 75 27 x 24 x 24" 214 (134) 

(69 x 61 x 61cm) 

w/Sound Cabinet 

OF302-4M 4 57 60 48 16 x 18 x 24" 99 (62) 

(41 x 46 x 61cm) 

OF302-25M 25 57 60 66 34 x 18 x 26" 172 (108) 

(86 x 46 x 66cm) 

2000-40M 40 120 130 52 34 x 25 x 26" 245 (153) 

(86 x 64 x 66cm) 

4000-40M 40 120 130 52 34 x 25 x 26" 245 (153) 

(86 x 64 x 66cm) 

Sound Cabinet and Air Dryer 

OF302-4MD2 4 46 48 48 16 x 18 x 28" 115 (72) 

(41 x 46 x 71cm) 

OF302-25MD2 25 46 48 48 34 x 18 x 26" 283 (177) 

(86 x 46 x 66cm) 

2000-40MD2 40 96 104 52 34 x 25 x 26" 256 (160) 

(86 x 64 x 66cm) 

4000-40MD3 40 96 104 52 34 x 25 x 26" 256 (160) 

(86 x 64 x 66cm) 

All compressors will go to 120psig maximum. 


Standard with Sound Cabinet—M Series 

M series compressors are mounted inside 

a metal sound reduction cabinet. The 

sound cabinet also comes with vibration 

damping and cooling. 

Standard Compressors Installed with Air Dryer 

and Sound Cabinet 

The compressors with air dryers 

and sound cabinet have a reduced 

maintenance schedule due to their auto 

drain feature. They have additional 

cooling fans to reduce heat. 

Air Compressors with Sound Cabinet* 

Description 

Part No. 

Model OF302-4M 89346 

Model OF302-25M 89347 

Model 2000-40M 89329 

Model 4000-40M 89274 



6706 

Air Compressor with Sound Cabinet* 

Description 

Part No. 

Model OF302-4MD2 89349 

Model OF302-25MD2 89350 

Model 2000-40MD2 89331 

Model 4000-40MD3 89276 



6707 


211



Equipment/Compressor Cross-Reference 

Equipment that Uses Compressors 

Model Flow Compressor 

Alltech ® ELSD Nitrogen Generator 

ELSD 4L/min OF302-25BD2 

Domnick Hunter ® LC/MS Generators 

LCMS30-1 30L/min 2000-40B 

LCMS30-0 30L/min 2000-40B 

Parker Balston ® LC/MS Generators 

N2-14 14-66L/min 2000-40B 

N2-22 14-66L/min 2000-40B 

Parker Balston ® UHP Nitrogen Generators 

HPN2-1100 500-1100mL/min 1000-25B 

UHPN2-1100 500-1100mL/min 2000-40B 

HPN2-2000 2L/min 2000-40B 

Parker ChromGas ® UHP Zero Air Generators 

1000 1.0L/min OF302-4B 

3500 3.5L/min OF302-4B 

Parker Balston ® Zero Air Generators 

75-83 1.0L/min OF302-4B 

HPZA-3500 3.5L/min OF302-4B 

HPZA-7000 7.0L/min OF302-4B 

HPZA-18000 18.0L/min 1000-25B 

HPZA-30000 30.0L/min 1000-25B 

Domnick Hunter ® Zero Air Generators 

UHP-10ZA 1.0L/min OF302-4B 



UHP-200ZA 20.0L/min 1000-25B 

UHP-350ZA 35.0L/min 1000-25B 

tech tip 

Choosing a tubing material 

A number of options are available for packed column tubing. 

The most inert material is glass, which should be used for active 

compounds. Glass-lined tubing and AT-Steel provide the inert 

surface of glass combined with the mechanical strength of a 

metal column. 

Metal tubing provides an economical and rugged column for 

suitable application. Passivated nickel tubing can frequently 

be used with active compounds such as phenols and amines. 

Stainless steel tubing is recommended for hydrocarbon, fixed 

gas, and solvent analyses where column inertness is less of 

a concern. 

PTFE tubing is extremely inert, but due to temperature limitations 

and poor column efficiency, PTFE is generally only recommended 

for the analysis of corrosive gases which are too reactive 

for glass. 


Stainless steel tubing 

We offer a full selection of lengths, IDs, 

and coating types to meet all of your 

GC tubing needs. See pages 386, 

388–389. 


Gas purifiers and traps 

A clean gas stream helps improve 

the quality of your GC analysis 

and the reliability of your results. 

For a full line of purifiers and 

traps in multiple styles, see pages 

266–270 and 277–279. 


Rotary tubing cutter 

Cut metal and glass-lined tubing with no 

deburring or reaming. See page 391. 


GC fittings 

For a full selection of stainless steel GC fittings, 

reducing unions, and other connectors, see pages 

282–285. 


Gas sampling bags 

We offer a broad selection of 

gas sampling bags in many 

sizes, materials, and shapes. 



Vials 

We have one of the largest selections 

of vials for all types of chromatography, 

including headspace vials, autosampler 

vials, and much more. See pages 348–378. 

5096 

4653 

5110 

5130 

6648 

4715 



Grace ® GC Capillary Column Families 

• Alltech ® Heliflex ® —High-Performance, Widest Selection of Phases 

• Alltech ® Econo-Cap—Best Value, Popular Phases 

Grace offers two lines of capillary columns to suit every 

application and budget; Alltech ® Heliflex ® and Alltech ® 

Econo-Cap. Heliflex ® Columns are Grace’s premium line of 

individually tested capillary columns. Econo-Cap Columns 

are batch tested to dramatically reduce the price without 

sacrificing quality. Both the Heliflex ® and Econo-Cap lines 

are manufactured under identical conditions using the same 

high-quality polyimide coated fused silica and immobilized 

stationary phases. In addition each are mounted on a 

rugged cage. 

Regardless of the capillary line you choose, every Grace 

manufactured capillary column has a guarantee of column 

performance. 

Alltech ® Heliflex ® Capillary Columns 

• High-performance individually 

tested columns 

• Choice of 5" or 7" rugged cage 

• Most complete selection 

of phases 

• Custom columns available to 

suit your specific application 

Heliflex ® Capillary Columns are manufactured from the 

highest quality polyimide coated synthetic fused silica. 

They are mounted on a rugged wear-resistant cage. Each 

Heliflex ® Column is individually tested to guarantee column 

performance and comes with a test chromatogram, instruction 

manual, capillary end caps, and a free ceramic column cutter. 

Note: Column nuts and ferrules are not included. If the 

column you need is not available in our standard offering, 

request a custom Heliflex ® Column made to your individual 

specifications. 

Alltech ® Econo-Cap Capillary Columns 

• Best value in capillary columns 

anywhere 

• Batch tested to dramatically 

reduce price 

• Ideal for aggressive applications 

• Available in the most popular 

phases 

premium 

value 

We’ve taken our most popular capillary columns and 

perfected the manufacturing procedure to the point that 

reproducibility is assured. This allows us to test these 

columns in batches instead of individually, while still offering 

a guarantee. Batch testing significantly reduces cost in 

production without sacrificing quality. 

Each Econo-Cap Capillary Column comes with a copy 

of the batch test results, an instruction manual, and capillary 

end caps. If you are not completely satisfied with the quality 

of your Econo-Cap Capillary Column it will be replaced 

at no charge. 

Available Alltech ® Heliflex ® Phases 

Phase 

Page 

AT-1ms 216 

AT-1ht 216 

AT-1 217 

AT-5ms 218 

AT-5 218 

AT-20 219 

AT-35ms 220 

AT-35 220 

AT-50 221 

AT-624 226 

AT-1301 221 

AT-1701 222 

AT-210 222 

AT-225 226 

AT-WAXms 223 

AT-WAX 223 

AT-AquaWax 224 

AT-AquaWax-DA 224 

AT-1000 225 

AT-FAME 225 

AT-CAM 225 

AT-Amino Acid 228 

Drug 1, Drug 2, and Drug 3 228 

USP Method 467 Kits 229 

AT-Pesticide 230 

AT-502.2 230 

AT-Petro 231 

AT-2887 231 

AT-3710 231 

AT-Sulfur 231 

AT-Amine 231 

AT-Silar-90 227 

AT-Silar-100 227 

Available Alltech ® Econo-Cap Phases 

Phase 

Page 

EC-1 217 

EC-5 219 

EC-20 219 

EC-WAX 224 

EC-1000 225 

tech tip 

Temperature limits 

Our GC capillary columns are temperature rated. In some cases, 

we list two maximum operating temperatures, the lower one is 

for isothermal condition and the higher one for temperatureprogrammed 

condition. 

5382 

gc columns | capillary 

Note: Column nuts and ferrules are not included. 


213


Capillary Column Phase Cross Reference 

Use the chart below to cross reference manufacturers’ stationary phases as well as USP method designations to Alltech 

equivalent columns. Slight variations in selectivity may result between different manufacturers’ columns. 

Alltech ® Standard Phase Cross Reference 


Standard Phase Cross Reference 

Composition 

Alltech Brand 

Usp 

Designation Similiar Phases 

100% Dimethylpolysiloxane AT-1ms G1, G2, G38 DB-1ms, HP-1ms, Rtx ® -1ms, CP-Sil 5CB low-bleed/ms 

100% Dimethylpolysiloxane AT-1ht, AT-1, 

EC-1 

G1, G2, G38 007-1, CP-Sil 5CB, DB-1, DB-1ht, HP-1, HP-101, 

OV-1, RSL-150, RSL-160, Rtx ® -1, SE-30, SPB-1, SPB-Sulfur, 

ULTRA-1, SP-2100, BP-1, DC-200, PE-1, ZB-1 

5% Phenyl equivalent 

Polysilphenylene-Siloxane 

AT-5ms 

DB-5ms, Rtx ® -5 Sil ms, BPX-5, 007-5ms, HP-5ms 

(5% Phenyl)- 

95% methylpolysiloxane 

(20% Phenyl)- 


(35 Phenyl)- 

65% Methylpolysiloxane 

(50 Phenyl)- 


(6% Cyanopropylphenyl)- 




Trifluoropropylmethyl 

polysiloxane 



Poly(80% biscyanopropyl)- 

20% cyanoproylphenyl siloxane 

Poly(biscyanopropyl 

siloxane) 

Polyethyleneglycol 


Acid Modified 

AT-5, 

EC-5 

AT-20, 

EC-20 

AT-35ms 

AT-35 

G27, 

G36 

G28, 

G32 

G42 

007-2, CP-Sil 8CB, DB-5, DB-5.625, DB-5ht, HP-5, 

HP-5ms, OV-5, PTE-5, PTE-5QTM, PAS-5, RSL-200, Rtx ® -5, Rtx ® -5ms, SAC-5, 

SE-54, SPB-5, ULTRA-2, XTI-5, SE-52, BP-5, PE-2, ZB-5 

007-7, Rtx ® -20, SPB-20, VOCOL, PE-7 

DB-35ms, Rtx ® -35, SPB-35, Sup-Herb, MDN-35, BPX-35 

007-11, DB-35, DB-35ms, HP-35, RSL-300, Rtx ® -35, SPB-35, SPB-608, 

OV-11, PE-35, Sup-Herb 

AT-50 G3 DB-17ms, H5-50+, Rtx ® -50, SP-2250, SPB-50, SPB-17, BPX-50 

DB-17ht, Rtx ® -65TG, BPX-50, CP-TAB-CB, 

007-17, DB-17, DB-17ht, HP-17, HP-50+, Rtx ® -50 

SP-50, SP-2250, SPB-50, CP-Sil 24CB, PE-17, ZB-50 

AT-624 

G43 007-1301, DB-624, DB-1301, HP-1301, HP-624, 

AT-1301 

Rtx ® -1301, Rtx ® -624, SPB-1301, SPB-624, 007-624, ZB-624 

AT-1701 

007-1701, CP-Sil 19CB, DB-1701, HP-1701, OV-1701, 

PAS-1701, Rtx ® -1701, SPB-1701, BP-10, ZB-1701 

AT-210 G6 DB-210, RSL-400, Rtx ® -200, OV-202, OV-210, OV-215, QF-1, SP-2401 

AT-225 G7 007-225, CP-Sil 43CB, DB-225, HP-225, OV-225, RSL-500, Rtx ® -225, 

BP-225, PE-225 

AT-SILAR-90 G8 DB-23, CP-Sil 84, Rtx ® -2330, SP-2330 

AT-SILAR-100 G5 CP-Sil 88, Rtx ® -2330, SP-2340 

AT-WAXms 

AT-WAX 

AT-AquaWax 

EC-WAX 

AT-1000, 

EC-1000, 

AT-AquaWax-DA 

G14, G15 

G16, G20, 

G39 

G25, 

G35 

DB-WAX, Rtx ® -WAX, Supelcowax-10 

007-CW, Carbowax ® 20M, CP-Wax 52CB, DB-WAXetr, 

DB-WAX, Rtx ® -WAX, HP-20M, HP-Wax, Innowax, 

Omegawax, Stabilwax ® , Supelcowax ® -10, Superox ® II, BP-20, ZB-WAX 

007-FFAP, CP-Wax 58CB, DB-FFAP, HP-FFAP, Nukol, 

OV-351, SP-1000, Stabilwax ® -DA, Superox ® -FA, BP-21 


AT-CAM Stabilwax ® -DB, CAM, Carbowax ® Amine, CP Wax 51 

Base Modified 

PONA Analysis AT-Petro 007-1-100-0.5F, CP-Sil PONA CB, DB-Petro100, Petrocol DH, PONA, 

Ttx-1PONA, HP-PONA 

ASTM Method 2887 AT-2887 007-1-10V-1.0F, DB-2887, Petrocol 2887, Petrocol EX2887, Rtx ® -2887, 

Sim-Dist-CB 

ASTM Method 3710 AT-3710 Petrocol 3710 

Alltech ® Specialty Columns Cross Reference 

Specialty Phases Cross Reference 

EPA Method 608 AT-Pesticide 007-608, DB-608, HP-608, SPB-608 

EPA Method 502.2 AT-502.2 007-624, 007-502, DB-502.2, DB-VRX, HP-624, 

HP-VOC, MST-502.2, MXT-624, MXT-Volatiles, OV-624, Rtx ® -502.2, Rtx ® -624, 

Rtx-Volatiles, VOCOL 

Sulfur Analysis AT-Sulfur SPB-1 Sulfur 

FAME Analysis AT-FAME FAMEWAX, OmegaWAX 



Alltech ® Custom Capillary Columns 

• A wide selection of stationary phases 

• Lengths from 1 to 105 meters 

• Wide selection of i.d. tubing 

• Every column individually tested 

Custom Capillary Column Instructions 

1. Stationary Phase: Choose a Phase in “Table 1: Custom 

Phase.” 

2. Length & Internal Diameter: Choose any length 

(1–105 meters) and internal diameter (0.10mm–0.53mm) 

in “Table 2: Custom Dimensions.” 

3. Film Thickness: Choose a film thickness; 0.01µm 

tolerance. 

4. See Coiling and Guard Information for additional options. 

5. Call Customer Service and they will confirm your order 

and provide exact prices. 

Table 1: Custom Phases 

Phases Available for Custom Capillary Columns 

• Aquawax 

• DC-550 

• Igepal CO-880 

• Aquawax-DA 

• Dexsil ® 300 

• OV-1 

• AT-1 


• OV-11 

• AT-5 


• OV-17 

• AT-20 

• Di (2ethylhexyl • OV-1701 

• AT-35 

phthalate 

• OV-61 

• AT-1000 

• Diisodecyl phthalate • OV-101 

• AT-1301 

• FFAP 

• OV-105 

• AT-1701 

• Hi-EFF-1AP • OV-210 

• AT-WAX 

• Hi-EFF-1BP • OV-351 

• AT-Amine 

• Hi-EFF-2AP • Polyvinylpyrrolidone 

• Apiezon ® phases • Hi-EFF-2BP • Reoplex 400 

• Apolane-87 • Hi-EFF-3AP • SE-30 

• Butanediol succinate • Hi-EFF-3BP • Squalane 

(Craig’s polyester) • Hi-EFF-4BP • Superox ® -FA 

• Carbowax ® phases • Hi-EFF-8BP • XE-60 

Table 2: Custom Dimensions 

Alltech ® Heliflex ® Custom Capillary Columns 

Length i.d. Description Part No. 

1– 5m 0.10mm Specify Phase and Film 971001 

0.18mm Specify Phase and Film 971005 





6 –15m 0.10mm Specify Phase and Film 971011 






16 – 30m 0.10mm Specify Phase and Film 971028 






31– 60m 0.18mm Specify Phase and Film 971050 





61–105m 0.25mm Specify Phase and Film 972105 




Custom Capillary 

All other Dimensions not specified 970123 

Custom Capillary Coiling 

Custom Coiling 

Description 

Part No. 

Custom Capillary on 4" Cage 970124 

Custom Capillary on 5" Cage 970125 






Custom Capillary Column with Integral Guard 

Custom Capillary Column with Integral Guard 

Description 

Part No. 

Custom 0.25mm i.d. with Integral 5m Guard C-5400 




215


Heliflex ® AT -1ms 

• Guaranteed low-bleed characteristics 

• Specifically designed for mass spec analysis 

• Highly inert 

• Similar to DB-1ms, HP-1ms, Rtx ® -1ms, 

CP-Sil 5CB low-bleed/MS, MDN-1 

AT-1ms is a line of high-performance low bleed nonpolar 

columns designed specifically for use with mass 

spectrometers. They are ideal for pharmaceutical, 

environmental, petrochemical, and food and fragrance 

analysis, or any GC/MS analysis requiring a 100% 

dimethylpolysiloxane-type polarity capillary column. 

premium 

Heliflex ® AT -1ht 

premium 

Shorten Your Analysis Time with . 

High-Temperature/Low-Bleed Columns 

• Faster analysis time for high boiling compounds 

• Better peak detection at lower concentrations 

• Individually bleed tested at 380ºC 

• Similar to 007-1, CP-Sil 5CB, DB-1, DB-1ht, HP-1, HP-101, 

OV TM -1, RSL-150, RSL-160, Rtx ® -1, SE-30, SPB TM -1, 

SPB TM -Sulfur, ULTRA-1, SP-2100, BP-1, DC-200, 

PE-1, ZB-1 


Heliflex ® AT-1ms Specifications 

Phase: 

100% Dimethylpolysiloxane 

Polarity: 

Non-polar 

Max. Temp.: 340/360°C 

USP Designation: G1, G2, G38 

Ideal For: 

Mass Spec Analysis for Environmental, PAH, CLP 

Pesticides, Steroids, and Drugs 

1 

4 

2 

3 

6 7 8 

5 

Figure 1 – AT-1ms Low Bleed 

9 

2578 

1. 2-Ethylhexanoic Acid 

2. 4-Chlorophenol 

3. 1,7-Heptanediol 

4. n-Tridecane 

5. 1-Undecanol 

6. n-Tetradecane 

7. Dicyclohexylamine 


9. n-Pentadecane 

Heliflex ® AT-1ht Specifications 

Phase: 


Polarity: 

Non-polar 

Max. Temp.: 380/400°C 


Ideal For: 

High Boiling Compounds Waxes, Crude Oils, and 

Trigylcerides 

1 

3 

2 

QC Test Chromatogram – Bleed Profile 

45 

2634 





5. Fluorene 

6. Anthracene 

7. n-Heptadecane 

3 pA 

0 5 10 15 20 25 30 35 40 Min. 

Column: Heliflex ® AT-1ms, 30m x 0.25mm x 0.25µm 

(Part No. 15897) 

Temp: 

135°C (20min hold) to 320°C (10min hold) at 

20°C/min 

Carrier Gas: Helium at 0.65mL/min (23.1cm/sec) 

Detector: FID 

3.4 pA 

0 5 10 15 20 25 30 35 40 45 50 Min. 

Column: Heliflex ® AT-1ht, 30m x 0.25mm x 0.10µm 

(Part No. 16368) 

Column Temp: 125ºC (25min hold) to 380ºC at 20ºC/min 

Carrier Gas: Helium at 26.3cm/sec 

Detector: FID at 390ºC 

Heliflex ® AT-1ms Capillary Columns 

Length i.d. Film 

Temp. Limits 

min.–max. Part No. 

15m 0.25mm 0.25µm -60–360°C 15880 

0.32mm 0.25µm -60–360°C 15882 

30m 0.25mm 0.10µm -60–360°C 15884 

0.25mm 0.25µm -60–360°C 15897 

0.25mm 0.25µm -60–360°C 15735* 

0.32mm 0.10µm -60–360°C 15922 

0.32mm 0.25µm -60–360°C 15938 

0.32mm 0.25µm -60–360°C 15739* 

60m 0.25mm 0.25µm -60–360°C 15941 

0.32mm 0.25µm -60–360°C 15944 

*Equipped with uncoated 5m integral guard column. Total column length is 35 meters. 

Heliflex ® AT-1ht GC Capillary Columns 


Temp. Limits* 

min.–max. 

Part No. 

15m 0.25mm 0.1µm -60–380/400°C 16367 

30m 0.25mm 0.1µm -60–380/400°C 16368 

60m 0.25mm 0.1µm -60–380/400°C 16369 

*Isothermal/Temp. program. 

more info 

For more Capillary GC Applications, See the GC Applications 

Section pages 462–493. 







Heliflex ® AT -1 

• Excellent general purpose columns 

• Similar to DB-1, Rtx ® -1, SPB-1, SPB-Sulfur, 

SP-2100, HP-1, HP-101, ULTRA-1, BP-1, CP-Sil 5CB, 

007-1, OV-1, SE-30, DC-200, RSL-150, RSL-160, 

PE-1, ZB-1 

Heliflex ® AT-1 Specifications 

Phase: 


Polarity: 

Non-polar General Purpose Column 


Ideal For: 

General Purpose 

premium 

Heliflex ® AT-1 Capillary Columns 


Temp. Limits 


10m 0.25mm 0.20µm ‐60–350°C 932510 

0.25mm 0.25µm ‐60–350°C 13752 

0.25mm 0.10µm ‐60–350°C 16866 

0.32mm 0.10µm ‐60–350°C 16868 

0.32mm 0.25µm ‐60–350°C 13755 

0.32mm 0.30µm ‐60–350°C 933210 

0.53mm 0.25µm ‐60–350°C 16840 

0.53mm 1.20µm ‐60–350°C 935110 

0.53mm 2.65µm ‐60–300°C 16849 

0.53mm 5.00µm ‐60–300°C 16842 

15m 0.25mm 0.10µm ‐60–350°C 13649 

0.25mm 0.20µm ‐60–350°C 932515 

0.25mm 0.25µm ‐60–350°C 13753 

0.25mm 1.00µm ‐60–350°C 13653 

0.32mm 0.10µm ‐60–350°C 13673 

0.32mm 0.25µm ‐60–350°C 13757 

0.32mm 0.30µm ‐60–350°C 933215 

0.32mm 1.00µm ‐60–350°C 13698 

0.32mm 3.00µm ‐60–350°C 13701 

0.32mm 5.00µm ‐60–300°C 16836 

0.53mm 0.15µm ‐60–350°C 13945 

0.53mm 0.25µm ‐60–350°C 14194 

0.53mm 0.50µm ‐60–350°C 14030 

0.53mm 1.00µm ‐60–350°C 13505 

0.53mm 1.20µm ‐60–350°C 935115 

0.53mm 1.50µm ‐60–330°C 13905 

0.53mm 2.65µm ‐60–300°C 16875 

0.53mm 3.00µm ‐60–300°C 13893 

0.53mm 5.00µm ‐60–300°C 16851 

25m 0.25mm 0.20µm ‐60–350°C 932525 

0.32mm 0.30µm ‐60–350°C 933225 

0.53mm 1.20µm ‐60–350°C 935125 

30m 0.25mm 0.10µm ‐60–350°C 13663 

0.25mm 0.20µm ‐60–350°C 932530 

0.25mm 0.25µm ‐60–350°C 13638 

0.25mm 1.00µm ‐60–350°C 13639 

0.32mm 0.10µm ‐60–350°C 13699 

0.32mm 0.25µm ‐60–350°C 13640 

0.32mm 0.30µm ‐60–350°C 933230 

0.32mm 1.00µm ‐60–350°C 13641 

0.32mm 3.00µm ‐60–300°C 13702 

0.32mm 5.00µm ‐60–300°C 16838 

0.53mm 0.50µm ‐60–350°C 14038 

0.53mm 1.00µm ‐60–350°C 13515 

0.53mm 1.20µm ‐60–350°C 935130 

0.53mm 1.50µm ‐60–330°C 13908 

30m 0.53mm 2.65µm ‐60–300°C 16876 

0.53mm 3.00µm ‐60–300°C 13897 

0.53mm 5.00µm ‐60–300°C 16843 



Temp. Limits 


50m 0.25mm 0.20µm ‐60–350°C 932550 

0.32mm 0.30µm ‐60–350°C 933250 

0.53mm 1.20µm ‐60–350°C 13938 

60m 0.25mm 0.10µm ‐60–350°C 13672 

0.25mm 0.25µm ‐60–350°C 13666 

0.25mm 1.00µm ‐60–350°C 13667 

0.32mm 0.10µm ‐60–350°C 13700 

0.32mm 0.25µm ‐60–350°C 13668 

0.32mm 1.00µm ‐60–350°C 13669 

0.32mm 3.00µm ‐60–300°C 13703 

0.32mm 5.00µm ‐60–300°C 13704 

0.53mm 0.50µm ‐60–350°C 13898 

0.53mm 1.00µm ‐60–350°C 13533 

0.53mm 1.20µm ‐60–350°C 13809 

0.53mm 1.50µm ‐60–330°C 13930 

0.53mm 3.00µm ‐60–300°C 13902 

0.53mm 5.00µm ‐60–300°C 13810 

100m 0.25mm 0.25µm ‐60–350°C 13972 

0.32mm 0.25µm ‐60–350°C 13979 

0.53mm 1.20µm ‐60–350°C 13982 

Heliflex ® AT-1 Capillary Columns on 5" Cage 

Temp. Limits 



15m 0.25mm 0.25µm ‐60–350°C 137535 

0.32mm 0.25µm ‐60–350°C 137575 

0.32mm 1.00µm ‐60–350°C 136985 

0.53mm 1.00µm ‐60–350°C 135055 

0.53mm 1.50µm ‐60–350°C 139055 

30m 0.25mm 0.25µm ‐60–350°C 136385 

0.32mm 0.25µm ‐60–350°C 136405 

0.32mm 1.00µm ‐60–350°C 136415 

0.53mm 1.00µm ‐60–350°C 135155 

0.53mm 1.50µm ‐60–350°C 139085 

Econo-Cap EC -1 

• Batch tested to dramatically reduce price 

value 

Econo-Cap EC-1 Capillary Columns 


Temp. Limits 


10m 0.53mm 2.65µm ‐60–350°C 19678 

15m 0.45mm 1.00µm ‐60–350°C 19642 

0.53mm 1.20µm ‐60–350°C 19650 

30m 0.25mm 0.25µm ‐60–350°C 19652 

0.32mm 0.25µm ‐60–350°C 19651 

0.32mm 1.00µm ‐60–350°C 19660 

0.45mm 1.00µm ‐60–350°C 19644 

0.53mm 1.00µm ‐60–350°C 19664 

0.53mm 1.20µm ‐60–350°C 19656 

best 

value 

Econo-Cap EC-1 Capillary Columns, Six Pack 

Temp. Limits 



30m 0.25mm 0.25µm ‐60–350°C 196526 

30m 0.32mm 0.25µm ‐60–350°C 196516 

30m 0.53mm 1.20µm ‐60–350°C 196566 



217



• Similar to DB-5ms, Rtx ® -5 Sil ms, 

HP-5TA, BPX-5, 007-5ms 

premium 

The AT -5ms capillaries are ideal for environmental, 

pharmaceutical, and petrochemical analysis, or any GC/MS 

analysis requiring a 5% phenyl-type polarity capillary column. 


• Excellent general purpose columns 

• Similar to DB-5, Rtx ® -5, SPB-5, HP-5, 

ULTRA-2, BP-5, CP-Sil 8CB, 007-2, OV-5, 

SE-52, SE-54, RSL-200, ZB-5 

premium 


Phase: 

(5% Phenyl)-95% Methoxpolysiloxane 

Polarity: 

Non-polar 

USP Designation: G27, G36 

Ideal For: 

Mass Spec Analysis—General Application 


Phase: 

(5% Phenyl)-95% Methylpolysiloxane 

Polarity: 

Non-polar 

Max. Temp.: 380/400°C 


Ideal For: 

General Applications 


1. TEPP 

2. Sulfotepp 

3. Monocrotophos 

4. Dimethoate 

5. Dioxathion 

6. Terbufos 

7. Phosphamidon 

8. Malathion 

9. Parathion 

10. cis-Chlorfenvinphos 

11. trans-Chlorfenvinphos 

12. Ethion 

Column: 

Temp: 

Carrier Gas: 

Detector: 

Organophosphorus Pesticides 

13. Famphur 

14. Carbophenothion 

15. Phosmet 

1 

2 

4 

6 

4 8 12 16 20 24 28 32 36 40 Min. 

Heliflex ® AT-5ms Capillary Columns 


Temp. Limits* 


15m 0.25mm 0.25µm -60–325/350°C 15801 

30m 0.25mm 0.10µm -60–325/350°C 15806 

0.25mm 0.25µm -60–325/350°C 15807 

0.25mm 0.25µm -60–325/350°C 15881* 

0.25mm 0.50µm -60–325/350°C 15808 

0.25mm 0.50µm -60–325/350°C 15889* 

0.25mm 1.00µm -60–325/350°C 15809 

0.32mm 0.10µm -60–325/350°C 15893 

0.32mm 0.25µm -60–325/350°C 15894 

0.32mm 0.25µm -60–325/350°C 15883* 

0.32mm 1.00µm -60–325/350°C 15895 

60m 0.25mm 0.10µm -60–325/350°C 15879 

0.25mm 0.25µm -60–325/350°C 15891 

0.25mm 1.00µm -60–325/350°C 15892 

0.32mm 0.25µm -60–325/350°C 15896 


3 

5 

7 

8 

9 

12 

14 

17 

11 13 15 18 

10 

2469 

16. EPN 

17. Leptophos 

18. Azinphos-ethyl 

Heliflex ® AT-5ms, 30m x 0.25mm x 0.25µm 

(Part No. 15807) 

120°C (3min hold) to 285°C at 5°C/min (4min hold) 

Helium, 26cm/sec 

MSD 

16 



Temp. Limits 


10m 0.25mm 0.20µm ‐60–350°C 952510 

0.32mm 0.30µm ‐60–350°C 953210 

0.53mm 0.25µm ‐60–350°C 16145 

0.53mm 1.20µm ‐60–350°C 955110 

0.53mm 2.65µm ‐60–300°C 16853 

0.53mm 5.00µm ‐60–300°C 16855 

15m 0.25mm 0.10µm ‐60–300°C 13705 

0.25mm 0.20µm ‐60–300°C 952515 

0.25mm 0.25µm ‐60–350°C 13758 

0.25mm 1.00µm ‐60–350°C 13706 

0.32mm 0.10µm ‐60–350°C 13709 

0.32mm 0.25µm ‐60–350°C 13887 

0.32mm 0.30µm ‐60–350°C 953215 

0.32mm 1.00µm ‐60–350°C 13710 

0.53mm 0.25µm ‐60–350°C 14196 

0.53mm 0.50µm ‐60–300°C 14039 

0.53mm 1.00µm ‐60–350°C 13569 

0.53mm 1.20µm ‐60–350°C 955115 

0.53mm 1.50µm ‐60–330°C 13906 

0.53mm 3.00µm ‐60–300°C 13894 

0.53mm 5.00µm ‐60–300°C 13896 

25m 0.25mm 0.20µm ‐60–350°C 952525 

0.32mm 0.30µm ‐60–350°C 953225 

0.53mm 1.20µm ‐60–350°C 955125 

30m 0.25mm 0.10µm ‐60–350°C 13707 

0.25mm 0.25µm ‐60–350°C 13656 

0.25mm 0.50µm ‐60–330°C 13309 

0.25mm 1.00µm ‐60–350°C 13657 

0.32mm 0.10µm ‐60–300°C 13711 

0.32mm 0.25µm ‐60–350°C 13658 

0.32mm 0.30µm ‐60–350°C 953230 

0.32mm 1.00µm ‐60–350°C 13659 

0.53mm 0.50µm ‐60–300°C 14045 

0.53mm 1.00µm ‐60–350°C 13798 

0.53mm 1.20µm ‐60–350°C 955130 

0.53mm 1.50µm ‐60–330°C 13909 

0.53mm 2.65µm ‐60–300°C 16857 

0.53mm 5.00µm ‐60–300°C 16859 

50m 0.25mm 0.20µm ‐60–350°C 952550 

0.32mm 0.30µm ‐60–300°C 953250 

0.53mm 1.20µm ‐60–350°C 13942 







Heliflex ® AT-5 Capillary Columns (continued) 

Temp. Limits 



60m 0.25mm 0.10um -60–350°C 13708 

0.25mm 0.20µm -60–350°C 952560 

0.25mm 0.25µm -60–350°C 13676 

0.25mm 1.00µm -60–350°C 13677 

0.32mm 0.10µm -60–350°C 13712 

0.32mm 0.25µm -60–350°C 13678 

0.32mm 0.30µm -60–350°C 953260 

0.32mm 1.00µm -60–350°C 13679 

0.53mm 0.50µm -60–350°C 13899 

0.53mm 1.00µm -60–350°C 14076 

0.53mm 1.20µm -60–350°C 13820 

0.53mm 1.50µm -60–330°C 13931 

0.53mm 3.00µm -60–350°C 13903 

0.53mm 5.00µm -60–300°C 13829 

100m 0.25mm 0.25µm -60–350°C 13973 

0.32mm 0.25µm -60–350°C 14011 

0.53mm 1.20µm -60–350°C 14019 


Temp. Limits 



15m 0.25mm 0.25µm -60–350°C 137585 

0.32mm 0.25µm -60–350°C 138875 

0.32mm 1.00µm -60–350°C 137105 

0.53mm 1.00µm -60–350°C 135695 

0.53mm 1.50µm -60–350°C 139065 

30m 0.25mm 0.25µm -60–350°C 136565 

0.32mm 0.25µm -60–350°C 136585 

0.32mm 1.00µm -60–350°C 136595 

0.53mm 1.00µm -60–350°C 137985 

0.53mm 1.50µm -60–350°C 139095 


• Unique polarity between non-polar and 

mid-range 

• Similar to 007-7, Rtx ® -20, SPB TM -20, VOCOL TM , PE-7 


Phase: 


Polarity: 

Intermediate Polarity 


Ideal For: 

Volatile Compounds and Solvents 

premium 



Temp. Limits 


10m 0.53mm 1.20µm ‐20–320°C 13936 

15m 0.25mm 0.25µm ‐20–320°C 13838 

0.32mm 0.25µm ‐20–320°C 13849 

0.53mm 1.20µm ‐20–320°C 13919 

0.53mm 1.00µm ‐20–300°C 13880 

25m 0.53mm 1.20µm ‐20–320°C 13934 

30m 0.25mm 0.25µm ‐20–320°C 13857 

0.32mm 0.25µm ‐20–320°C 13859 

0.53mm 1.00µm ‐20–300°C 13882 

0.53mm 1.20µm ‐20–300°C 13932 

50m 0.53mm 1.20µm ‐20–320°C 13944 

60m 0.25mm 0.25µm ‐20–320°C 13868 

0.32mm 0.25µm ‐20–320°C 13869 

0.53mm 1.00µm ‐20–300°C 13884 

0.53mm 3.00µm ‐20–280°C 13324 




value 



value 



Temp. Limits 


15m 0.45mm 1.00µm -60–350°C 19661 

0.53mm 1.20µm -60–350°C 19645 

30m 0.25mm 0.25µm -60–350°C 19647 

0.32mm 0.25µm -60–350°C 19646 

0.32mm 1.00µm -60–350°C 19665 

0.53mm 1.00µm -60–350°C 19671 

0.53mm 1.20µm -60–350°C 19657 

best 

value 

Econo-Cap EC-5 Capillary Columns, Six Pack 

Temp. Limits 



15m 0.25mm 0.25µm -60–350°C 19649 

30m 0.25mm 0.25µm -60–350°C 196476 

0.32mm 0.25µm -60–350°C 196466 

0.53mm 1.20µm -60–350°C 196576 



Temp. Limits 


15m 0.45mm 1.00µm ‐20–300°C 19670 

0.53mm 1.20µm ‐20–300°C 19673 

6515 


Parker Balston ® FID gas station 

The Parker Balston ® FID gas station combines 

Parker’s zero air and hydrogen generation 

technologies into a single unit, eliminating the 

inconveniences and cost of hydrogen and gas 

cylinders. See page 208. 


219



premium 

• Guaranteed low-bleed 

• Specifically designed for mass spec analysis 

• Similar to DB-35ms, Rtx ® -35, SPB TM -35, Sup-Herb TM , 

MDN-35, BPX-35 


• Ideal column for conformational analysis 

• Similar to DB-35, DB-35ms, Rtx ® -35, BPX-35, 

SPB-35, SPB-608, HP-35, 007-11, OV-11, 

RSL-300, PE-35, Sup-Herb TM , ZB-35 

premium 


Phase: 


Polarity: 


USP Designation: G42 

Ideal For: 

Pesticides, Herbicides, Drugs, Aromatics 


Phase: 


Polarity: 


USP Designation: G42 

Ideal For: 

Pesticides, Herbicides, Drugs, Aromatics 


Heliflex ® AT-35ms Capillary Column 


Temp. Limits 


15m 0.25mm 0.25µm -40–340°C 12588 

30m 0.25mm 0.25µm -40–340°C 12589 

60m 0.25mm 0.25µm -40–340°C 12590 

Tocopherols 

0 1 2 3 4 5 6 Min. 

Column: Heliflex ® AT-35, 25m x 0.25mm x 0.20µm 

(Part No. 962525) 

Temp: 310°C 

Carrier: 

Hydrogen at 40cm/sec 

Detector: FID 

1 

2 

3 

4 

1273 

1 . d-δ-Tocopherol 

2 . d-β-Tocopherol 

3 . d-γ-Tocopherol 

4 . d-α-Tocopherol 



Temp. Limits 


10m 0.25mm 0.25µm -20–320°C 19613 

0.32mm 0.10µm -20–350°C 16872 

0.32mm 0.25µm -20–300°C 13759 

0.53mm 0.10µm -20–350°C 16861 

0.53mm 0.20µm -20–280°C 16865 

0.53mm 1.20µm -20–300°C 965110 

0.53mm 2.65µm -20–280°C 16877 

15m 0.25mm 0.25µm -20–320°C 13943 

0.25mm 0.50µm -20–320°C 13713 

0.32mm 0.15µm -20–280°C 13714 

0.32mm 0.25µm -20–320°C 13760 

0.32mm 0.50µm -20–320°C 13091 

0.53mm 1.00µm -20–300°C 14129 

0.53mm 1.20µm -20–300°C 965115 

25m 0.25mm 0.20µm -20–320°C 962525 

0.32mm 0.30µm -20–320°C 963225 

0.53mm 1.20µm -20–300°C 965125 

30m 0.25mm 0.15µm -20–320°C 13432 

0.25mm 0.20µm -20–300°C 962530 

0.25mm 0.25µm -20–320°C 13642 

0.25mm 0.50µm -20–320°C 13643 

0.25mm 1.00µm -20–300°C 13796 

0.32mm 0.15µm -20–320°C 13716 

0.32mm 0.25µm -20–320°C 13644 

0.32mm 0.50µm -20–320°C 13645 

0.53mm 0.50µm -20–320°C 13797 

0.53mm 1.00µm -20–300°C 14001 

0.53mm 1.20µm -20–300°C 965130 

0.53mm 2.65µm -20–280°C 16878 

50m 0.25mm 0.20µm -20–320°C 962550 

0.32mm 0.30µm -20–320°C 963250 

60m 0.25mm 0.20µm -20–320°C 962560 

0.25mm 0.25µm -20–320°C 13682 

0.25mm 0.50µm -20–320°C 13683 

0.32mm 0.25µm -20–320°C 13684 

0.32mm 0.50µm -20–320°C 13685 

0.53mm 1.00µm -20–320°C 14070 

more info 

For more Capillary GC Applications, See the GC Applications 

Section pages 462–493. 




• General purpose column 

• Similar to DB-17, DB-17ht, Rtx ® -50, BPX TM -50, 

SPB TM -50, SP-2250, HP-50+, HP-17, 007-17, 

OV-17, CP-Sil 24CB, PE-17, ZB-50 

premium 


• Unique polarity makes it ideal for a range 

of applications 

• Similar to BP624, DB-1301, Rtx ® -1301, Rtx ® -624, 

HP-1301, HP-624, DB-624, 007-624, 007-1301, 

SPB-1301, SPB TM -624, ZB-624 

premium 


Phase: 

(50% Phenyl)-Methylpolysiloxane 

Polarity: Intermediate Polarity 

USP Code: G3 

Ideal For: Pesticides, Herbicides, Phthalate Esters, Free Phenols, 

and Basic Drugs 


Phase: 

(6% Cyanopropylpenyl)-Methylpolysiloxane 


USP Code: G43 

Ideal For: Volatile Organics, Pharmaceuticals, and 

EPA Method 612 



Temp. Limits 


15m 0.25mm 0.25µm 40–325°C 13837 

0.32mm 0.25µm 40–325°C 13856 

0.53mm 1.00µm 40–280°C 13876 

30m 0.25mm 0.25µm 40–325°C 13839 

0.32mm 0.25µm 40–325°C 13858 

0.53mm 0.50µm 40–300°C 13991 

0.53mm 1.00µm 40–280°C 13878 


Stainless steel tubing 

We offer a full selection of lengths, IDs, 

and coating types to meet all of your 

GC tubing needs. See pages 386, 

388–389. 

5096 



Temp. Limits 


30m 0.25mm 0.25µm -20–280°C 13959 

0.25mm 1.00µm -20–260°C 13962 

0.32mm 0.25µm -20–280°C 14123 

0.53mm 1.00µm -20–260°C 13970 

0.53mm 3.00µm -20–260°C 15562 

more info 

For Capillary GC Applications, see the GC Applications Section, 

pages 462–493. 







GC fittings 

For a full selection of stainless steel GC fittings, 

reducing unions, and other connectors, see pages 

282–285. 

5130 






Vials 

We have one of the largest selections 

of vials for all types of chromatography, 

including headspace vials, autosampler 

vials, and much more. See pages 348–378. 

4715 


221



• Similar to DB- 210, Rtx ® -200, OV- 202, 

OV-210, OV-215, QF-1, SP-2401, RSL-400 

premium 


• Similar to DB-1701, OV-1701, SPB-1701, 

Rtx ® -1701, 007-1701, PAS-1701, CP-Sil 19CB, 

HP-1701, BP-10, ZB-1701 

premium 


Phase: Trifluoropropyl Methylpolysiloxane 

Polarity: Polar 

USP Code: G6 

Ideal For: Ketones, Aldehydes, Silanes, Glycols, Nitro Aromatics, 

Herbicides, and Method 8140 and 609 


Phase: 

(14% Cyanopropylphenyl)-86% Methylpolysiloxane 


USP Code: n/a 

Ideal For: Pesticides, PCB’s, Drugs, Herbicides, and TMS Sugars 




Temp. Limits 


10m 0.53mm 1.20µm 45–240°C 985110 

15m 0.32mm 0.15µm 45–260°C 13724 

0.32mm 0.25µm 45–260°C 13888 

0.53mm 1.00µm 45–240°C 13127 

0.53mm 1.20µm 45–240°C 985115 

25m 0.53mm 1.20µm 45–240°C 985125 

30m 0.32mm 0.15µm 45–260°C 13726 

0.32mm 0.25µm 45–260°C 13840 

0.32mm 0.50µm 45–260°C 13727 

0.53mm 1.00µm 45–240°C 13136 

0.53mm 1.20µm 45–240°C 985130 

60m 0.32mm 0.30µm 45–260°C 983260 

1. Monochloroacetic Acid 

2. Monobromoacetic Acid 

3. Dalapon 

4. Dichloroacetic Acid 

5. Trichloroacetic Acid 

6. Bromochloroacetic Acid 

7. Dibromoacetic Acid 

0 

Column: 

Temp: 

Carrier Gas: 

Detector: 

EPA Method 552.1: Haloacetic Acids 

1 

4 

2 

3 

2 4 6 8 10 12 14 16 18 Min. 

5 

6 

7 

2105 

Heliflex ® AT-1701, 30m x 0.32mm x 0.25µm 

(Part No. 13690) 

50°C (10min hold) to 120°C at 10°C/min 

Helium at 35cm/sec 

ECD 



Temp. Limits 


10m 0.25mm 0.20µm -20–280°C 942510 

0.53mm 1.20µm -20–280°C 945110 

15m 0.25mm 0.25µm -20–280°C 13881 

0.25mm 1.00µm -20–280°C 13718 

0.32mm 0.25µm -20–280°C 13762 

0.32mm 1.00µm -20–280°C 13720 

0.53mm 0.50µm -20–280°C 14046 

0.53mm 1.00µm -20–280°C 14074 

0.25mm 0.20µm -20–280°C 942515 

0.32mm 0.15µm -20–280°C 13719 

0.53mm 1.20µm -20–280°C 945115 

25m 0.25mm 0.20µm -20–280°C 942525 

0.32mm 0.30µm -20–280°C 943225 

0.53mm 1.20µm -20–280°C 945125 

30m 0.25mm 0.15µm -20–280°C 13417 

0.25mm 0.25µm -20–280°C 13686 

0.25mm 1.00µm -20–280°C 13687 

0.32mm 0.25µm -20–280°C 13690 

0.32mm 0.25µm -20–280°C 13106* 

0.32mm 1.00µm -20–280°C 13691 

0.53mm 0.50µm -20–280°C 14105 

0.53mm 1.00µm -20–280°C 13795 

0.53mm 1.20µm -20–280°C 13830 

50m 0.25mm 0.20µm -20–280°C 942550 

0.32mm 0.30µm -20–280°C 943250 

60m 0.25mm 0.15µm -20–280°C 13404 

0.25mm 0.25µm -20–280°C 13688 

0.25mm 1.00µm -20–280°C 13689 

0.32mm 0.15µm -20–280°C 13434 

0.32mm 0.25µm -20–280°C 13692 

0.32mm 1.00µm -20–280°C 13693 

0.53mm 0.50µm -20–280°C 14107 

0.53mm 1.00µm -20–280°C 14131 

*10-meter integral guard column. 


Temp. Range 



15m 0.25mm 0.25µm -20–280°C 138815 

0.32mm 0.25µm -20–280°C 137625 

0.32mm 1.00µm -20–280°C 137205 

0.53mm 1.00µm -20–280°C 140745 

0.53mm 1.20µm -20–280°C 9451155 

30m 0.25mm 0.25µm -20–280°C 136865 

0.32mm 0.25µm -20–280°C 136905 

0.32mm 1.00µm -20–280°C 136915 

0.53mm 1.00µm -20–280°C 137955 

0.53mm 1.20µm -20–280°C 138305 



Heliflex ® AT -WAXms 

• Designed to provide low MS bleed 

• Similar to DB-WAX, Rtx ® -WAX, 

Supelcowax TM -10 

Heliflex ® AT-WAXms Specifications 

Phase: 

100% Polyethylene Glycol 


USP Code: G14, G15, G16, G39 

Ideal For: Mass Spec Analysis 

Heliflex ® AT-WAXms Capillary Columns 


Temp. Limits 


15m 0.25mm 0.25µm 40–260°C 12603 

0.32mm 0.25µm 40–260°C 12606 

0.53mm 1.00µm 40–260°C 12610 

30m 0.25mm 0.25µm 40–260°C 12604 

0.32mm 0.25µm 40–260°C 12607 

0.53mm 1.00µm 40–260°C 12611 

60m 0.25mm 0.25µm 40–260°C 12605 

0.32mm 0.25µm 40–260°C 12608 

Heliflex ® AT -WAX 

premium 

premium 

• Similar to DB-WAX, DB-WAXetr, Rtx ® -WAX, 

Stabilwax ® , Carbowax ® 20M, Supelcowax TM -10, 

Innowax, HP-20M, HP-Wax, BP-20, CP-Wax 52-CB, 

007-CW, Superox ® II, OmegaWAX, ZB-WAX 

Heliflex ® AT-WAX Specifications 

Phase: 



USP Code: G14, G15, G16, G39 

Ideal For: FAMEs, Polar Solvents, BTEX, Flavor and Fragrances, 

Glycols, Alcohols, and Aromatics 

Heliflex ® AT-WAX Capillary Columns (continued) 

Temp. Limits 



15m 0.32mm 2.50µm 40–260°C 137372 

0.53mm 0.50µm 40–260°C 14109 

0.53mm 1.00µm 40–260°C 13904 

0.53mm 1.20µm 40–260°C 9951152 

0.53mm 150µm 40–250°C 13215 

0.53mm 2.50µm 40–250°C 16881 

0.53mm 5.00µm 40–250°C 16883 

25m 0.25mm 0.20µm 40–280°C 9925252 

0.32mm 0.30µm 40–280°C 9932252 

0.53mm 1.20µm 40–260°C 9951252 

30m 0.25mm 0.15µm 40–280°C 13418 

0.25mm 0.20µm 40–280°C 9925302 

0.25mm 0.25µm 40–280°C 136462 

0.25mm 0.50µm 40–260°C 136472 

0.32mm 0.25µm 40–280°C 136482 

0.32mm 0.30µm 40–280°C 9932302 

0.32mm 0.50µm 40–260°C 136522 

0.32mm 1.00µm 40–260°C 137382 

0.53mm 0.50µm 40–260°C 14111 

0.53mm 1.00µm 40–260°C 13907 

0.53mm 1.20µm 40–260°C 9951302 

0.53mm 1.50µm 40–260°C 13109 

0.53mm 2.00µm 40–250°C 16162 

0.53mm 2.50µm 40–250°C 16885 

0.53mm 5.00µm 40–250°C 16887 

50m 0.25mm 0.20µm 40–280°C 9925502 

0.32mm 0.30µm 40–280°C 9932502 

0.53mm 1.20µm 40–260°C 13946 

60m 0.25mm 0.20µm 40–280°C 9925602 

0.25mm 0.25µm 40–280°C 136942 

0.25mm 0.50µm 40–260°C 13916 

0.32mm 0.25µm 40–280°C 136962 

0.32mm 0.30µm 40–280°C 9932602 

0.32mm 0.50µm 40–260°C 136972 

0.53mm 0.50µm 40–260°C 13901 

0.53mm 1.00µm 40–260°C 13929 

0.53mm 1.20µm 40–260°C 13850 

0.53mm 1.50µm 40–260°C 13243 


Heliflex ® AT-WAX Capillary Columns 


Temp. Limits 


10m 0.25mm 0.25µm 40–280°C 19615 

0.25mm 0.20µm 40–280°C 9925102 

0.32mm 0.25µm 40–280°C 13778 

0.53mm 1.20µm 40–260°C 9951102 

15m 0.25mm 0.15µm 40–280°C 13433 

0.25mm 0.20µm 40–280°C 9925152 

0.25mm 0.25µm 40–280°C 13777 

0.25mm 0.50µm 40–260°C 137302 

0.25mm 1.00µm 40–260°C 137312 

0.32mm 0.25µm 40–280°C 13779 

0.32mm 0.30µm 40–280°C 9932152 

0.32mm 0.50µm 40–260°C 137352 

0.32mm 1.00µm 40–260°C 137362 

Heliflex ® AT-WAX Capillary Columns on 5" Cage 

Temp. Limits 



15m 0.25mm 0.25µm 40–280°C 137755 

0.32mm 0.25µm 40–280°C 137785 

0.32mm 1.00µm 40–260°C 1373625 

0.53mm 1.00µm 40–260°C 139045 

0.53mm 1.20µm 40–260°C 99511525 

30m 0.25mm 0.25µm 40–280°C 1364625 

0.32mm 0.25µm 40–280°C 1364825 

0.32mm 1.00µm 40–260°C 1373825 

0.53mm 1.00µm 40–260°C 139075 

0.53mm 1.20µm 40–260°C 99513025 






223



Econo-Cap EC -WAX 


• For phase specifications, see 

Heliflex ® AT version 

Econo-Cap EC-WAX Capillary Columns 

Temp. Limits 



15m 0.45mm 1.00µm 40–260°C 19667 

0.53mm 1.00µm 40–260°C 19674 

0.53mm 1.20µm 40–260°C 19653 

30m 0.25mm 0.25µm 40–280°C 19655 

0.32mm 0.25µm 40–280°C 19654 

0.45mm 1.00µm 40–260°C 19663 

0.53mm 1.00µm 40–260°C 19676 

0.53mm 1.20µm 40–260°C 19659 

Econo-Cap EC-WAX Six-Pack Capillary Columns 

Temp. Limits 



30m 0.25mm 0.25µm 40–260°C 196556 

0.32mm 0.25µm 40–260°C 196546 

0.53mm 1.20µm 40–260°C 196596 

more info 

For more information on the Econo-Cap Capillary Line, refer to 

page 213. 

Heliflex ® AT -AquaWax 

• Designed and tested for aqueous injections 

• Similar to DB-Wax, DB-Waxer, Rtx ® -WAX, 

Stabilwax ® , HP-Wax, HP-20M, HP-Innowax, 

ZB-Wax, and Supelcowax TM -10 

value 

best 

value 

premium 

Heliflex ® AT -AquaWax-DA 

• Designed and tested for aqueous injections 

• Eliminates the need for derivatization of 

acidic organic compounds 

• Similar to DB-FFAP, Stabilwax ® , HP-FFAP-DA, 

ZB-FFAP, SPB TM -1000, and Nukol 

Heliflex ® AT-AquaWax-DA Specifications 

Phase: 100% Polyethylene Glycol—Nitroterephthal Acid Modified 


USP Code: n/a 

Ideal For: Analysis of Acidic Organic Compounds and Free 

Fatty Acids 

Heliflex ® AT-AquaWax-DA Capillary Columns 

Temp. Limits 



30m 0.25mm 0.25µm 40–250°C 14537 

0.32mm 0.25µm 40–250°C 14539 

0.53mm 1.00µm 40–250°C 14543 

60m 0.25mm 0.25µm 40–250°C 14547 

0.32mm 0.25µm 40–250°C 14549 

1 

3 

2 

Free Fatty Acids 

4 5 

6 

premium 

2528 

1. Propionic Acid 

2. Butyric Acid 

3. Valeric Acid 

4. Caproic Acid 

5. Heptanoic Acid 

6. Caprylic Acid 

Heliflex ® AT-AquaWax Specifications 

Phase: 



USP Code: n/a 

Ideal For: Food and Beverage Analysis 

0 

2 4 6 8 10 Min. 

Heliflex ® AT-AquaWax Capillary Columns 

Temp. Limits 



30m 0.25mm 0.25µm 40–260°C 12437 

0.32mm 0.25µm 40–260°C 12439 

60m 0.25mm 0.25µm 40–260°C 12447 

0.32mm 0.25µm 40–260°C 12449 





Column: Heliflex ® AT-AquaWax-DA, 30m x 0.25mm x 

0.25µm (Part No. 14537) 

Temp: 180°C 

Carrier Gas: Helium at 22cm/sec 

Detector: FID 

more info 


pages 462–493. 




• Similar to DB-FFAP, Stabilwax ® -DA, 

SPB-1000, Nukol, HP-FFAP, BP-21, 

CP-Wax-58-CB, 007-FFAP, OV-351, FFAP, 

Superox ® -FA, ZB-FFAP 

premium 

Heliflex ® AT -CAM 

• Eliminates need for derivitazation 

• Similar to Stabilwax ® -DB, Carbowax ® Amine, 

CAM, CP-Wax 51 

premium 


Phase: 

100% Polyethylene Glycol—Acid Modified 

Polarity: High Polarity 

USP Code: G25, G35 

Ideal For: Free Fatty Acids 



Temp. Limits 


10m 0.25mm 0.20µm 40–250°C 972510 

0.32mm 0.30µm 40–250°C 973210 

0.53mm 1.20µm 40–250°C 975110 

15m 0.25mm 0.20µm 40–250°C 972515 

0.25mm 0.25µm 40–250°C 13782 

0.32mm 0.25µm 40–250°C 13886 

0.32mm 0.30µm 40–250°C 973215 

0.53mm 1.00µm 40–250°C 13873 

0.53mm 1.20µm 40–250°C 975115 

25m 0.25mm 0.20µm 40–250°C 972525 

0.32mm 0.30µm 40–250°C 973225 

0.53mm 1.20µm 40–250°C 975125 

30m 0.25mm 0.20µm 40–250°C 972530 

0.25mm 0.25µm 40–250°C 13783 

0.32mm 0.25µm 40–250°C 13785 

0.32mm 0.30µm 40–250°C 973230 

0.53mm 1.00µm 40–250°C 13877 

0.53mm 1.20µm 40–250°C 975130 

0.53mm 2.00µm 40–250°C 13017 

50m 0.25mm 0.20µm 40–250°C 972550 

0.32mm 0.30µm 40–250°C 973250 

60m 0.25mm 0.20µm 40–250°C 972560 

0.25mm 0.25µm 40–250°C 13784 

0.32mm 0.25µm 40–250°C 13786 

0.32mm 0.30µm 40–250°C 973260 

0.32mm 1.00µm 40–250°C 13276 

0.53mm 1.00µm 40–250°C 13879 

Heliflex ® AT-CAM Specifications 

Phase: 

100% Polyethylene Glycol—Base Modified 



Ideal For: Basic Compounds, Amines 

Heliflex ® AT-CAM Capillary Columns 


Temp. Limits 


30m 0.32mm 0.25µm 40–250°C 13024 

0.53mm 0.50µm 40–250°C 13026 

0.53mm 1.00µm 40–250°C 13028 

Heliflex ® AT -FAME 

• Similar to FAMEWAX and OmegaWAX 

Heliflex ® AT-FAME Specifications 

Phase: 

Stable Bonded Polyethylene Glycol 



Ideal For: Fatty Acid Methyl Esters 

Heliflex ® AT-FAME Capillary Columns 


Temp. Limits 


30m 0.25mm 0.25µm 40–280°C 12436 

0.32mm 0.25µm 40–280°C 12438 








• For phase specifications, see Heliflex ® AT version 

value 


Temp. Limits 



15m 0.45mm 1.00µm 40–250°C 19648 

0.53mm 1.20µm 40–250°C 19684 

30m 0.25mm 0.25µm 40–250°C 19686 

0.32mm 0.25µm 40–250°C 19685 

0.53mm 1.00µm 40–250°C 19692 

0.53mm 1.20µm 40–250°C 19688 

best 

value 

Econo-Cap EC-1000 Six-Pack Capillary Columns 

Temp. Limits 



30m 0.25mm 0.25µm 40–250°C 196866 

0.32mm 0.25µm 40–250°C 196856 

0.53mm 1.20µm 40–250°C 196886 


225



premium 

• Specially tested for EPA Method 624 

• Similar to DB-1301, Rtx ® -1301, 

Rtx ® -624, HP-1301, HP-624, DB-624, 

007-624, 007-1301, SPB-1301, SPB-624, ZB-624 


• Similar to DB-225, HP-225, 

Rtx ® -225, OV-225, RSL-500, 007-225, 

CP-Sil 43CB, BP-225, PE-225 

premium 


Phase: 

6% Cyanopropylphenyl-94% Methylpolysiloxane 


USP Code: G43 

Ideal For: EPA Methods 524, 601, 602, 624, 8240 and 8260, and 

Solvent Analysis 


Phase: 


Polarity: Mid to High Polarity 

USP Code: G19 

Ideal For: Carbohydrates and Solvents 




Temp. Limits 


30m 0.25mm 1.40µm -20–260°C 13754 

0.32mm 1.80µm -20–260°C 13756 

0.32mm 3.00µm -20–260°C 13780 

0.53mm 3.00µm -20–260°C 16889 

60m 0.25mm 1.40µm -20–260°C 13503 

0.32mm 1.80µm -20–260°C 13799 

0.53mm 3.00µm -20–260°C 13800 

75m 0.53mm 3.00µm -20–260°C 13937 

EPA Method 602 — Purgeable Aromatics 

1 

2 

3 4 5 

1233 

6 1. Benzene 

2. Toluene 

3. Chlorobenzene 

4. Ethylbenzene 

5. 1,3-Dichlorobenzene 

7 





Temp. Limits 


15m 0.25mm 0.20µm 40–240°C 912515 

0.25mm 0.25µm 40–240°C 13883 

0.32mm 0.25µm 40–240°C 13774 

25m 0.25mm 0.20µm 40–240°C 912525 

0.53mm 1.20µm 40–220°C 915125 

30m 0.25mm 0.25µm 40–240°C 13773 

0.32mm 0.25µm 40–240°C 13775 

0.32mm 0.30µm 40–240°C 913230 

0.53mm 1.20µm 40–220°C 915130 

6515 


Parker Balston ® FID gas station 

The Parker Balston ® FID gas station combines 

Parker’s zero air and hydrogen generation 

technologies into a single unit, eliminating the 

inconveniences and cost of hydrogen and gas 

cylinders. See page 208. 

0 2 4 6 8 10 12 14 16 Min. 





Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 13756) 


Helium at 1.1mL/min 

FID 







Heliflex ® AT -Silar-90 

• Designed and tested for FAME 

• Similar to DB-23, CP-Sil 84, Rtx ® -2330, SP-2330 

premium 

Heliflex ® AT -Silar-100 

• Ideal for high and low temperature separation of 

samples containing geometric isomers of FAMEs 

• Similar to CO-Sil 88, Rtx ® -2330, SP-2340 

premium 

Heliflex ® AT-Silar-90 Specifications 

Phase: 

Poly (90% bis Cyanopropyl)-10% Cyanopropyphenyl 

Siloxane 


USP Code: G8 

Ideal For: Analysis of FAMEs 

Heliflex ® AT-Silar-100 Specifications 

Phase: 

Poly bis Cyanopropyl 

Polarity: High Polarity Available 

USP Code: G8 

Ideal For: cis/trans Isomers of FAMEs, Dioxins, and Furans 

Heliflex ® AT-Silar-90 Capillary Columns 


Temp. Limits 


15m 0.25mm 0.20µm 0–250°C 12627 

0.32mm 0.20µm 0–250°C 12629 

30m 0.25mm 0.20µm 0–250°C 12628 

0.32mm 0.20µm 0–250°C 12630 

1 

2 

3 

Fatty Acid Methyl Esters 

4 5 10 

9 

6 

7 8 

11 

12 

1. C14:0 

2. C16:0 

3. C16:1 

4. C18:1 

5. C18:2 

6. C18:3 

7. C18:3 

8. C20:1 

9. C20:3 

13 

14 

2678 

10. C20:4 

11. C20:5 

12. C22:4 

13. C22:5 

14. C22:6 

Heliflex ® AT-Silar-100 Capillary Columns 

Temp. Limits 



15m 0.25mm 0.20µm 0–250°C 12631 

0.32mm 0.20µm 0–250°C 12633 

30m 0.25mm 0.20µm 0–250°C 12632 

0.32mm 0.20µm 0–250°C 12634 

1 


in Rapeseed Oil 

2 

3 

4 5 

6 

7 

8 

1. C14:0 

2. C16:0 

3. C18:0 

4. C18:1 

5. C18:2 

6. C20:0 

9 

10 

2679 

7. C20:1 

8. C18:3 

9. C22:0 

10. C22:1 


0 

2 4 6 8 10 12 14 16 18 20 22 24 26 Min. 

0 

2 4 6 8 10 12 Min. 

Column: Heliflex ® AT-Silar-90, 30m x 0.25mm x 0.20µm 

(Part No. 24019) 

Temp: 200°C 


Detector: FID 

Column: Heliflex ® AT-Silar-100, 30m x 0.25mm x 0.20µm 

(Part No. 24022) 

Temp: 190°C 


Detector: FID 

tech tip 

Tips for Heliflex ® AT-Silar-90 and Silar-100 

Silar-90 and Silar-100 are more susceptible to damage by 

oxygen, moisture, and HCL than other silicone phases. Avoid 

solvents such as water and methanol when using on-column 

injection techniques. Columns should not be rinsed. 

more info 


pages 462–493. 


227


Heliflex ® AT -Amino Acid 

• Separates all 20 essential amino acids 

Heliflex ® AT-Amino Acid Specifications 

Phase: 

Proprietary 

Polarity: Intermediate 

USP Code: n/a 

Ideal For: Amino Acids 

premium 

Heliflex ® Drug 

Analysis Capillaries 

• No derivatization of sample necessary 

• Specially tested for drugs 

premium 

Drug One is a non-polar column that will elute drugs in 

the same order as an OV-1 or OV-101 packed column. 

Drug Two is a moderately polar column that will give a 

retention order similar to OV-17. Drug Three is a special 

phase formulated for the analysis of anticonvulsants. 


Heliflex ® AT-Amino Acid Capillary Column 

Temp. Limits 



25m 0.53mm 1.20µm -20–350°C 16864 

N(O,S)-Heptafluorobutyl-isobutyl Esters of Amino 

Acids from Soy Protein Hydrolysate 

1. Alanine 

2. Glycine 

3. Valine 

4. Threonine 

5. Serine 

6. Leucine 

1 

2 

3 

4 

7. Isoleucine 

8. Proline 

9. Cystine 




6 

5 

7 

8 

9 

10 


14. Lysine 

15. Tyrosine 

16. Arginine 

17. Histidine 

18. Tryptophan 

0 5 10 15 20 25 30 35 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 

11 

12 

13 

14 

15 

16 

17 18 

1288 

Heliflex ® AT-Amino Acid, 25m x 0.53mm x 1.20µm 

(Part No. 16864) 


Helium at 10mL/min 

FID 

Drug Specifications 

Phase: 

Proprietary 

Polarity: Drug 1—Non-Polar, Drug 2—Intermediate Polarity, 

Drug 3—Polar 

Temp. Limit: 330°C Isothermal/350°C Programmed 

USP Code: n/a 

Ideal For: Drug Analysis 

Heliflex ® Drug Capillary Columns 

Length Coating i.d. Film Temp. Limits* Part No. 

10m Drug One 0.53mm 1.20µm 330/350°C 16844 

Drug Two 0.53mm 1.20µm 330/350°C 16845 

Drug Three 0.53mm 1.20µm 330/350°C 16847 

30m Drug One 0.25mm 0.25µm 330/350°C 13745 

Drug Two 0.25mm 0.25µm 330/350°C 13746 

Drug One 0.32mm 0.25µm 330/350°C 13748 

Drug Two 0.32mm 0.25µm 330/350°C 13750 


0 

4 

1 

3 

2 

8 

7 

5 

6 

10 

9 

4 8 12 

11 12 13 

Drug Mixture 

16 Min. 

0042 

1. Methylenedioxymethamphetamine 

(MDMA) 

2. Benzocaine 

3. Meperidine 

4. Phencyclidine (PCP) 

5. Caffeine 

6. Procaine 

7. Mepivacaine 

8. Methadone 

9. Atropine 

10. Cocaine 

11. Oxazepam 

12. Codeine 

13. Diazepam 

more info 


pages 462–493. 

Column: 

Temp: 

Carrier Gas: 

Detector: 

Heliflex ® Drug Two, 30m x 0.25mm x 0.25µm 

(Part No. 13746) 

230°C to 250°C at 5°C/min 


FID 





tech tip 





condition. 



Heliflex ® USP 467 

Capillary Kits 

• Tested for organic volatile impurities 

• Kits for Method I and V for Method 467 

• Guard columns plus connector 

premium 

USP Method 467 deals with the determination of organic 

volatile impurities in pharmaceuticals, which results from 

solvents commonly used in the manufacturing process. 

Method I Kit Contains: 

AT-5, (USP G27) 30m x 0.53mm x 5.00µm; 5 x Universal 

FSOT Connectors; and 5 x 5m Deactivated Guard Columns 

(0.53mm i.d.). 

Method V Kit Contains: 

AT-624, (USP G43) 30m x 0.53mm x 3.00µm; 5 x Universal 

FSOT Connectors; and 5 x 5m Deactivated Guard Columns 

(0.53mm i.d.). 

Method I Kit Specifications 

Phase: 


Polarity: Non-Polar 

Temp. Limit: -60–350°C 

USP Code: G27 


3 

5 

USP 467 - Trace Volatiles 

8 

1797 

1. Ethylene Oxide 

2. Impurity 

3. Methylene Chloride 

4. Chloroform 

5. Benzene 

6. Trichloroethylene 

7. 1,4-Dioxane 

8. Dimethyl Sulfoxide 

5 6 

Method V Kit Specifications 

Phase: 



Temp. Limit: 20–260°C 

USP Code: G43 


USP 467 - Method V 

1866 

4 



2 

3. Chloroform 

4. Benzene 




7 

1 

12 

4 

6 

3 

0 3 6 9 12 15 18 Min. 


(Part No. 16859) 

Temp: 

35°C (5 min hold) to 175°C at 8°C/min 


Detector: FID at 260°C 

0 4 8 12 16 20 Min. 


(Part No. 16889) 

Temp: 40°C 


Detector: FID 

more info 

For more information on Pharmaceutical analysis, 

request brochure #470. 

USP 467 Capillary Kits 

Description 

Part No. 

Capillary Kits 

USP 467/Method I Capillary Kit 13963 

USP 467/Method V Capillary Kit 13971 

Replacement Components 

AT-5, 30m x 0.53mm x 5.0µm 16859 

AT-624, 30m x 0.53mm x 3.0µm 16889 

Universal FSOT Connectors, 5/pk 20400 

Deactivated Guard Columns, 5m x 0.53mm, 5/pk 13990 


229


Heliflex ® AT -Pesticide 

• Unique selectivity for pesticide confirmation 

• Pretested with pesticide mix 

premium 

Heliflex ® AT -502.2 

• Separates early eluting gases at above 

ambient temperature 

• Useful for volatile organics analysis, including EPA 

Methods 502.2, 524.2, 8260 

premium 

Heliflex ® AT-Pesticide Specifications 

Phase: 

Proprietary 


USP Code: n/a 

Ideal For: Chlorinated Pesticides, PCBs 

Heliflex ® AT-502.2 Specifications 

Phase: Proprietary 


USP Code: n/a 

Ideal For: Volatile Organics and EPA Methods 502.2, 524.2, and 8260 


Heliflex ® AT-Pesticide Capillary Columns 

Temp. Limits 



20m 0.53mm 0.60µm 40–240°C 16846 

30m 0.25mm 0.25µm 40–240°C 13662 

0.32mm 0.25µm 40–240°C 13744 

0.53mm 0.60µm 40–240°C 13969 

1 

2 

4 

6 

5 

3 

Chlorinated Pesticides 

7 

9 10 

8 11 

13 12 

14 

15 

16 

1. α-BHC 

2. γ-BHC 

3. β-BHC 

4. δ-BHC 

1569 

5. Heptachlor 

6. Aldrin 

7. Heptachlor Epoxide 

8. Endosulfan I 

9. p,p'-DDE 

10. Dieldrin 

11. Endrin 

12. p,p'-DDD 

13. Endosulfan II 

14. p,p'-DDT 

15. Endrin Aldehyde 

16. Endosulfan Sulfate 

Heliflex ® AT-502.2 Capillary Columns 


Temp. Limit 

max. 

Part No. 

30m 0.25mm 1.40µm 270°C 13787 

0.32mm 1.80µm 270°C 13789 

0.53mm 3.00µm 270°C 13792 

60m 0.25mm 1.40µm 270°C 13788 

0.32mm 1.80µm 270°C 13790 

0.53mm 3.00µm 270°C 13793 

105m 0.32mm 1.80µm 270°C 13791 

0.53mm 3.00µm 270°C 13794 

1 

2 

3 

4 

5 

6 

7 

TCLP Volatiles Mix 

8 

9 

10 

2064 

1. Vinyl Chloride 

2. 1,1-Dichloroethene 

3. 2-Butanone 

4. Chloroform 

5. Carbon Tetrachloride 

6. Benzene + 1,2-Dichloroethene 

7. Trichloroethene 

8. Tetrachloroethene 



0 4 8 12 16 20 Min. 

0 

3 6 9 12 15 18 21 24 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 

Heliflex ® AT-Pesticide, 30m x 0.25mm x 0.25µm 

(Part No. 13662) 

190°C to 240°C at 3°C/min 


ECD 

Column: 

Temp: 

Carrier Gas: 

Detector: 

Heliflex ® AT-502.2, 30m x 0.53mm x 3.00µm 

(Part No. 13792) 



FID 

more info 


pages 462–493. 


Need sample prep for your pesticide 

analysis? 

Florisil ® -PR is pretested specifically 

chlorinated pesticides, see page 309. 




• For simulated distillation of petroleum 

products 

premium 

Heliflex ® AT -Sulfur 

• Designed for the analysis of volatile sulfurcontaining 

compounds in petroleum distillates 

premium 


Phase: 



USP Code: n/a 

Ideal For: Petroleum Distillation 

Heliflex ® AT-Sulfur Specifications 

Phase: 



USP Code: n/a 

Ideal For: Volatile Sulfur Compounds 

Heliflex ® AT-2887 Capillary Column 


Temp. Limit 

max. 

Part No. 

10m 0.53mm 1.20µm 350°C 13947 

Test Mix for AT-2887 41719 

Heliflex ® AT-Sulfur Capillary Columns 


Temp. Limit 

min.–max. 

Part No. 

30m 0.32mm 4.00µm -20–350°C 14029 


• For boiling range of gasoline samples 


Phase: 



USP Code: n/a 

Ideal For: Boiling Point of Gasoline 

premium 

Heliflex ® AT-3710 Capillary Column 


Temp. Limit 

max. 

Part No. 

15m 0.53mm 5.00µm 300°C 13949 

Test Mix for AT-3710 41718 

Heliflex ® AT -Amine 

• Separates underivatized amines with no tailing 

Heliflex ® AT-Amine Specifications 

Phase: 

Proprietary—Non-bonded 


USP Code: n/a 

Ideal For: Amino Acids 

premium 

Heliflex ® AT-Amine Capillary Columns 


Temp. Limit 

max. 

Part No. 

30m 0.25mm 0.25µm 200°C 13314 

0.32mm 0.25µm 200°C 13316 

0.53mm 0.25µm 200°C 13318 

60m 0.25mm 0.25µm 200°C 13315 

0.32mm 0.25µm 200°C 13317 

0.53mm 0.25µm 200°C 13319 


Heliflex ® AT -Petro 

• For high resolution of complex 

hydrocarbon mixtures 

premium 

more info 

For more information about Petrochemical analysis, 

request brochure #478. 

Heliflex ® AT-Petro Specifications 

Phase: 



USP Code: n/a 

Ideal For: hydrocarbon Mixtures 

Heliflex ® AT-Petro Capillary Column 


Temp. Limit 

max. 

Part No. 

100m 0.25mm 0.50µm 350°C 13948 

more info 


pages 462–493. 






231



Alltech Column Guide to EPA Methods 

Alltech Guide to Capillary Columns for Environmental Analysis 

Method Description and Recommended Columns Comments Part No. 

501.3—Trihalomethanes in Drinking Water 

Heliflex ® AT-624, 30m x 0.53mm x 3.00µm 16889 

502.1—Volatile Halogenated Organics by Purge and Trap GC/ELCD 

Heliflex ® AT-624, 30m x 0.53mm x 3.00µm Subambient 16889 

Heliflex ® AT-624, 60m x 0.53mm x 3.00µm Ambient 13800 

502.2—Volatile Organics in Water by Purge and Trap GC/PID/ELCD 

Heliflex ® AT-502.2, 105m x 0.53mm x 3.00µm 13794 


DB-624, 75m x 0.53mm x 3.00µm 125-1374 

504—DBE and DBCP by Microextraction GC/ECD 

DX-3, 30m x 0.32mm x 0.25µm 123-6332 

Econo-Cap EC-1, 30m x 0.32mm x 1.00µm (Inexpensive, Batch QC’ed Column) 19660 

505—Organohalide Pesticides and PCBs by Microextraction and GC/ECD 

Heliflex ® AT-1, 30m x 0.32mm x 1.00µm Primary Column 13641 

DX-3, 30m x 0.32mm x 0.25µm Alternate Column 123-6332 

506—Phthalate and Adipate Esters in Water (GC/PID) 



507—Nitrogen and Phosphorus Containing Pesticides by GC/NPD 



Heliflex ® AT-1701, 30m x 0.25mm x 0.25µm Confirmation Column 13686 

508—Chlorinated Pesticides by GC/ECD 



Heliflex ® AT-1701, 30m x 0.25mm x 0.25µm Alternate Column 13686 

Heliflex ® AT-Pesticide, 30m x 0.32mm x 0.25µm 13744 


513—2, 3, 7, 8 Tetrachlorodibenzo-p-Dioxin 

DB-Dioxin, 60m x 0.25mm x 0.15µm 122-2461 

DB-Dioxin, 60m x 0.32mm x 0.15µm 123-2461 

515.1—Chlorinated Acids by GC/ECD 


Econo-Cap EC-5 Econo-Cap, 30m x 0.25mm x 0.25µm (Inexpensive, batch QC’ed column) 19647 

Heliflex ® AT-1701, 30m x 0.25mm x 0.25µm Confirmation Column 13686 

524.2—Purgeables by Purge and Trap GC/MS 

Heliflex ® AT-624, 30m x 0.53mm x 3.00µm Subambient (10°C) 16889 


Heliflex ® AT-5, 30m x 0.32mm x 1.00µm Subambient, Cryofocusing 13659 


525—Organic Compounds in Water by Liquid-Solid Extraction and Capillary GC/MS 

Heliflex ® AT-5ms, 30m x 0.32mm x 0.25µm 15894 


548—Endothall in Drinking Water 



551—Chlorination Disinfection By-products and Chlorinated Solvents in Drinking Water 




552.1—Haloacetic Acids in Drinking Water 



556.1—Carbonyl Compounds in Drinking Water 

Heliflex ® AT-5, 10m x 0.10mm x 0.10µm Fast GC 14438 

Heliflex ® AT-1701, 10m x 0.10mm x 0.10µm Fast GC 14439 



Alltech Column Guide to EPA Methods (continued) 

Alltech Guide to Capillary Columns for Environmental Analysis (continued) 


601—Purgeable Halocarbons by Purge and Trap ELCD 

Heliflex ® AT-624, 30m x 0.53mm x 3.00µm Subambient 16889 


602—Purgeable Aromatics by Purge and Trap GC/PID 


Heliflex ® AT-WAX, 30m x 0.53mm x 2.50µm Plus xylenes 16885 

Econo-Cap EC-WAX, 30m x 0.53mm x 1.20µm (Inexpensive, Batch QC’ed Column) 19659 

603—Acrolein and Acrylonitrile by GC/FID 


604—Phenols by GC/FID and GC/ECD 







606—Phthalate Esters by GC/ECD 



607—Nitrosamines 



608—Organochlorine Pesticides and PCBs by GC/ECD 




609—Nitroaromatics and Isophorone by GC/FID and GC/ECD 

Heliflex ® AT-5, 15m x 0.53mm x 1.20µm (Capillary GC) 955115 

610—Polynuclear Aromatic Hydrocarbons by GC/FID 




610—Polynuclear Aromatic Hydrocarbons by HPLC and GC/FID 





611—Haloethers by GC/HSD 



612—Chlorinated Hydrocarbons by GC/ECD 



615—Chlorinated Herbicides by GC/ECD/ELCD 



619—Triazine Herbicides 


624—Purgeables by Purge and Trap GC/MS 





625—Base/Neutral and Acids by GC/MS See Method 8270 — 

680—Pesticides and PCBs in Water and Soil/Sediment by GC/MS 


8011- EDB and DBCP by Microextraction Using GC 




233



Alltech Column Guide to EPA Methods (continued) 

Alltech Guide to Capillary Columns for Environmental Analysis (continued) 


8015—Nonhalogenated Volatile Organics 

Heliflex ® AT-WAX, 30m x 0.53mm x 1.00µm 13907 


8021—Halogenated Volatiles Using GC/PID/ELCD 


8032—Acrylamide by GC/ECD 


8033—Acetonitrile by GC/NPD 

Heliflex ® AT-WAX, 15m x 0.53mm x 1.00µm 13904 

8041—Phenols by GC/FID/(ECD) 



8070—Nitrosamines 



8081—Organochlorine Pesticides by GC/ECD 







8082—Polychlorinated Biphenyls (PCBs) by GC/ECD or GC/ELCD 







8091—Nitroaromatics and Cyclic Ketones by GC/ECD or GC/NPD 



8100—Polynuclear Aromatic Hydrocarbons by GC/FID 







8111—Haloethers by GC/ECD 



8141—Organophosphorus Pesticides by GC/NPD/FPD/ELCD 

Heliflex ® AT-210, 15m x 0.53mm x 1.20µm Column 1 985115 

Heliflex ® AT-5, 15m x 0.53mm x 1.20µm Column 2 955115 


8151—Chlorinated Herbicides by GC/ECD Using Methylation and Pentafluorobenzylation Derivatization 







8260—Volatile Organics by GC/MS: Capillary Column Technique 





Heliflex ® AT-502.2, 60m x 0.53mm x 3.00µm Ambient 13793 

Heliflex ® AT-502.2, 105m x 0.53mm x 3.00µm Ambient 13794 



Astec Chiraldex ® GC Capillary Columns 

• Resolve aromatic and nonaromatic enantiomers 

• Pure cyclodextrin derivative coating 

• No racemization of chiral stationary phase at elevated 

temperatures 

Chiraldex ® capillaries are based on derivatized cyclodextrins, 

which are chiral in nature formed by the α-1,4 linkage of 

glucose units into toroidal shaped structures. The enzyme 

cyclodextrin glucosyl transferance (CGT) cleaves partially 

digested starch and links the glucose units into predominantly 

three forms, referred to as alpha, beta, and gamma; 6, 7, 

and 8 glucose units, respectively. These cyclodextrins are 

thermally stable, highly crystalline and virtually insoluble in 

most organic solvents. Using the three forms of cyclodextrin, 

Astec has manufactured derivatives that exhibit properties 

allowing them to be used as GC phases. They are: 

– Permethylated Hydroxypropyl (PH) 

– Dialkylated (DA) 

– Trifluoroacetylated (TA) 

– Propionylated (PN) 

– Butyrylated (BP) 

– Permethylated (PM) 

– Dimethylated (DM) 

– Dipropionyl (DP) 

The prefix A, B, G describes the cyclodextrins alpha, 

beta, and gamma, respectively. The suffix describes the 

nature of the derivatization i.e., G-TA refers to Gamma 

Trifluororacetylated phase. 

The most striking characteristic of these phases is that they 

separate nonaromatic enantiomers including saturated 

alcohols, amines, carboxylic acids, epoxides, diols, polyols, 

cyclic, bicyclic, heterocyclic compounds, lactones, amino 

alcohols, amino acids, halohydrocarbons, α-halocarboxylic 

esters, pyrans, and furans. Little functionality is required for 

chiral recognition (Table 1). Also, there can be reversals of 

elution order from one series to the next as well as from one 

cavity size (beta) to another (gamma). The acylating reagent 

also can contribute to stereo-selectivity. 





Table 1—General Classes of Compounds Separated on Chiraldex Capillary Columns 

Chiraldex ® A-PH: Smaller linear and saturated amines, alcohols, carboxylic acids, and epoxides 

Chiraldex ® B-PH: Most structural types of compounds including linear and cyclic amines and alcohols, carboxylic acids, lactones, amino alcohols, sugars, 

bicyclics, epoxides, haloalkanes, and more 

Chiraldex ® G-PH: Cyclic and bicyclic diols and other larger compounds including steroids and carbohydrates 

Chiraldex ® A-DA: Smaller cyclic amines, alcohols, and epoxides (Inquire regarding availability) 

Chiraldex ® B-DA: Nitrogen heterocyclics, heterocyclics, some bicyclics, and epoxides, lactones, aromatic amines, sugars, amino acid derivatives 

Chiraldex ® G-DA: Aromatic amines containing 2 or more rings, large cyclic diols, some heterocyclics, multi-ring compounds or compounds with bulky 

substituents 

Chiraldex ® A-TA: Smaller alcohols, amino alcohols, amino alkanes, and diols 

Chiraldex ® B-TA: Broad range alkyl alcohols, halo acid esters, amino alkanes, halocycloalkanes, certain lactones, diols, alkyl halides, furan and pyran 

derivatives 

Chiraldex ® G-TA: >350 pairs chiral alcohols, diols, polyols, hydrocarbons, lactones, amine alcohols, halocarboxylic acid esters, homologous series, furan 

and pyran derivatives, epoxides, glycidyl analogs, and haloepihydrins 

Chiraldex ® G-PN: Epoxides, higher alcohols >C4, lactones 

Chiraldex ® G-BP: Amino acids, certain primary amines and furans 

Chiraldex ® G-DM: Selectivity similar to PM and PH, but with shorter retention times, and greater resolution. Especially applicable to aliphatic, olefenic, and 

aromatic enantiomers 

Chiraldex ® B-PM: Acids, alcohols, barbitals, diols, epoxides, esters, hydrocarbons, ketones, lactones, and terpenes 

Chiraldex ® B-DM: Selectivity similar to PM and PH, but with shorter retention times, and greater resolution. Especially applicable to aliphatic, olefenic, and 

aromatic enantiomers 

Chiraldex ® B-DP: Aromatic and aliphatic amines and some aliphatic and aromatic esters 

Chiraldex ® G-DP: Aromatic and aliphatic amines and some aliphatic and aromatic esters 



235



Astec Chiraldex ® GC Capillary Columns 

Chiraldex ® Capillary Columns 

Phase Length i.d. Film Temp. Limits* Part No. 

B-PM 30m 0.25mm 0.25µm 230/250°C 4711 

50m 0.25mm 0.25µm 230/250°C 4713 

A-TA 10m 0.25mm 0.125µm 180/180°C 4123 

20m 0.25mm 0.125µm 180/180°C 4125 

30m 0.25mm 0.125µm 180/180°C 4127 

40m 0.25mm 0.125µm 180/180°C 4405 

50m 0.25mm 0.125µm 180/180°C 4635 

B-TA 10m 0.25mm 0.125µm 180/180°C 4129 

20m 0.25mm 0.125µm 180/180°C 4131 

30m 0.25mm 0.125µm 180/180°C 4133 

40m 0.25mm 0.125µm 180/180°C 4407 

50m 0.25mm 0.125µm 180/180°C 4637 

G-TA 10m 0.25mm 0.125µm 180/180°C 4135 

20m 0.25mm 0.125µm 180/180°C 4137 

30m 0.25mm 0.125µm 180/180°C 4139 

40m 0.25mm 0.125µm 180/180°C 4409 

50m 0.25mm 0.125µm 180/180°C 4639 

A-PH 10m 0.25mm 0.125µm 200/220°C 4073 

20m 0.25mm 0.125µm 200/220°C 4088 

30m 0.25mm 0.125µm 200/220°C 4089 

40m 0.25mm 0.125µm 200/220°C 4393 

50m 0.25mm 0.125µm 200/220°C 4197 

B-PH 10m 0.25mm 0.125µm 200/220°C 4090 

20m 0.25mm 0.125µm 200/220°C 4092 

30m 0.25mm 0.125µm 200/220°C 4094 

40m 0.25mm 0.125µm 200/220°C 4395 

50m 0.25mm 0.125µm 200/220°C 4199 

G-PH 10m 0.25mm 0.125µm 200/220°C 4096 

20m 0.25mm 0.125µm 200/220°C 4098 

30m 0.25mm 0.125µm 200/220°C 4101 

40m 0.25mm 0.125µm 200/220°C 4397 

50m 0.25mm 0.125µm 200/220°C 4202 


Chiraldex ® GC Kits 

Chiraldex ® 3 column kits include 1 each of any three columns 

of the same i.d. and length at an economical price. We 

recommend as a starter kit for the broadest range 

of applications a Chiraldex ® -30 Kit including 1 each of 

B-DA, BDM, G-TA. Please note that Chiraldex ® B-PM is 

not included in the Chiraldex ® kits. 

Chiraldex ® GC Kits 

Phase Length i.d. Part No. 

Chiraldex ® -10 Kit 10m 0.25mm 4207 





Chiraldex ® Capillary Columns 

Phase Length i.d. Film Temp. Limits* Part No. 

A-DA 10m 0.25mm 0.125µm 200/220°C 4104 

20m 0.25mm 0.125µm 200/220°C 4107 

30m 0.25mm 0.125µm 200/220°C 4109 

40m 0.25mm 0.125µm 200/220°C 4399 

50m 0.25mm 0.125µm 200/220°C 4614 

B-DA 10m 0.25mm 0.125µm 200/220°C 4111 

20m 0.25mm 0.125µm 200/220°C 4113 

30m 0.25mm 0.125µm 200/220°C 4115 

40m 0.25mm 0.125µm 200/220°C 4401 

50m 0.25mm 0.125µm 200/220°C 4626 

G-DA 10m 0.25mm 0.125µm 200/220°C 4117 

20m 0.25mm 0.125µm 200/220°C 4119 

30m 0.25mm 0.125µm 200/220°C 4121 

40m 0.25mm 0.125µm 200/220°C 4403 

50m 0.25mm 0.125µm 200/220°C 4627 

G-PN 10m 0.25mm 0.125µm 200/220°C 4660 

20m 0.25mm 0.125µm 200/220°C 4664 

30m 0.25mm 0.125µm 200/220°C 4668 

40m 0.25mm 0.125µm 200/220°C 4672 

50m 0.25mm 0.125µm 200/220°C 4676 

G-BP 10m 0.25mm 0.125µm 200/220°C 4662 

20m 0.25mm 0.125µm 200/220°C 4680 

30m 0.25mm 0.125µm 200/220°C 4670 

40m 0.25mm 0.125µm 200/220°C 4674 

50m 0.25mm 0.125µm 200/220°C 4678 

G-DM 10m 0.25mm 0.25µm 230/250°C 5550 

20m 0.25mm 0.25µm 230/250°C 5551 

30m 0.25mm 0.25µm 230/250°C 5552 

40m 0.25mm 0.25µm 230/250°C 5599 

50m 0.25mm 0.25µm 230/250°C 5688 

B-DM 10m 0.25mm 0.12µm 230/250°C 4718 

20m 0.25mm 0.12µm 230/250°C 4719 

30m 0.25mm 0.12µm 230/250°C 4727 

40m 0.25mm 0.12µm 230/250°C 4728 

50m 0.25mm 0.12µm 230/250°C 4729 

B-DP 10m 0.25mm 0.125µm 200/220°C 41159 

20m 0.25mm 0.125µm 200/220°C 41160 

30m 0.25mm 0.125µm 200/220°C 41193 

40m 0.25mm 0.125µm 200/220°C 41165 

50m 0.25mm 0.125µm 200/220°C 41166 

G-DP 10m 0.25mm 0.125µm 200/220°C 41173 

20m 0.25mm 0.125µm 200/220°C 41174 

30m 0.25mm 0.125µm 200/220°C 41175 

40m 0.25mm 0.125µm 200/220°C 41176 

50m 0.25mm 0.125µm 200/220°C 41177 


tech tip 

Temperature Limits 




condition. 



SGE ® BPX Capillary Columns 

• Extended temperature range 

• Low bleed ideal for GC/MS applications 

SGE ® uses silphenylene-siloxane chemistry to manufacture 

BPX stationary phases, resulting in extremely low bleed, 

thermally stable capillary columns. 

BPX Specifications 

Phase Phase Composition 

BPX1-SimD 


BPX5 

5% Phenyl, 95% Methylpolysiloxane 

BPX35 


BPX50 


BPX70 

70% Cyanopropyl 

BPX90 

90% Cyanopropyl Polysilphenylene-siloxane 

BPX 608 

35% Phenyl Polysilphenylene-siloxane 

BPX Volatiles 

Cyanopropylphenyl Polysiloxane 

BPX1-SimD Capillary Columns 


Temp. Limits 


5m 0.53mm 0.1µm 30–430°C 54800* 

10m 0.53mm 0.9µm 30–400°C 54801** 

0.53mm 2.65µm 30–370°C 54802** 

*Aluminum Clad. **Polyimide Clad. 

BPX5 Capillary Columns 


Temp. Limits* 

min.–max. 

Part No. 

15m 0.25mm 0.25µm -40–360/370°C 54103 

0.25mm 1.00µm -40–360/370°C 54121 

0.32mm 0.25µm -40–360/370°C 54144 

0.32mm 1.00µm -40–360/370°C 54152 

0.53mm 1.00µm -40–360/370°C 54147 

0.53mm 3.00µm -40–350/360°C 54159 

30m 0.25mm 0.25µm -40–360/370°C 54101 

0.25mm 0.50µm -40–360/370°C 541025 

0.25mm 1.00µm -40–360/370°C 54122 

0.32mm 0.25µm -40–360/370°C 54145 

0.32mm 0.50µm -40–360/370°C 541205 

0.32mm 1.00µm -40–360/370°C 54153 

0.53mm 0.50µm -40–360/370°C 541345 

0.53mm 1.00µm -40–360/370°C 54148 

0.53mm 1.50µm -40–360/370°C 541348 

0.53mm 3.00µm -40–350/360°C 54160 

60m 0.25mm 0.25µm -40–360/370°C 54102 

0.25mm 1.00µm -40–360/370°C 54123 

0.32mm 0.25µm -40–360/370°C 54146 

0.32mm 1.00µm -40–360/370°C 54154 

0.53mm 1.00µm -40–360/370°C 54158 


BPX35 Capillary Columns (continued) 


Temp. Limits* 

min.–max. 

Part No. 

30m 0.25mm 0.25µm 0–360/370°C 54701 

0.25mm 0.50µm 0–360/370°C 547025 

0.25mm 1.00µm 0–360/370°C 54704 

0.32mm 0.25µm 0–360/370°C 54724 

0.32mm 0.50µm 0–360/370°C 547158 

0.32mm 1.00µm 0–360/370°C 54717 

0.53mm 0.50µm 0–360/370°C 54735 

0.53mm 1.00µm 0–360/370°C 54737 

60m 0.25mm 0.25µm 0–360/370°C 54702 

0.25mm 1.00µm 0–360/370°C 54705 

0.32mm 0.25µm 0–360/370°C 54725 




Temp. Limits* 

min.–max. 

Part No. 

15m 0.25mm 0.25µm 40–360/370°C 54750 

0.32mm 0.25µm 40–360/370°C 54760 

0.53mm 0.50µm 40–360/370°C 54769 

30m 0.25mm 0.25µm 40–360/370°C 54751 

0.32mm 0.25µm 40–360/370°C 54761 

0.53mm 0.50µm 40–360/370°C 54771 




Temp. Limits* 

min.–max. 

Part No. 

15m 0.25mm 0.25µm 50–260/290°C 54621 

0.53mm 0.50µm 50–260/290°C 54619 

30m 0.25mm 0.25µm 50–260/290°C 54622 

0.32mm 0.25µm 50–260/290°C 54616 

0.53mm 0.50µm 50–260/290°C 54620 

60m 0.25mm 0.25µm 50–260/290°C 54623 

0.32mm 0.25µm 50–260/290°C 54617 

120m 0.25mm 0.25µm 50–260/290°C 54624 


1 

2 

EPA Method 625 — Phenols 

5 

3 

4 

6 

7 

8 

9 10 11 

1871 

1. Phenol 


3. 2,4-Dinitrophenol 

4. 2-Nitrophenol 

5. 2,4-Dichlorophenol 

6. 4-Chloro-3-methylphenol 

7. 2,4,6-Trichlorophenol 



10. 2-Methyl-4,6-dinitrophenol 

11. Pentachlorphenol 




Temp. Limits* 

min.–max. 

Part No. 

15m 0.25mm 0.25µm 0–360/370°C 54700 

0.25mm 1.00µm 0–360/370°C 54703 

0.32mm 0.25µm 0–360/370°C 54723 

0.32mm 1.00µm 0–360/370°C 54716 

0.53mm 0.50µm 0–360/370°C 54734 

0.53mm 1.00µm 0–360/370°C 54736 


more info 

This is a partial listing of SGE ® Capillary Columns available from 

Grace. To view Grace’s complete chromatogram library, visit 

www.discoverysciences.com/chromdb/. 

0 1 2 3 4 5 6 7 8 9 10 11 12 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 

SGE ® BPX-5, 25m x 0.32mm x 0.50µm 

(Part No. 52318) 

125°C to 250°C (5min hold) at 15°C/min 

Hydrogen at 1.4mL/min 

FID 


237



SGE ® BPX Capillary Columns 

(continued) 



Temp. Limits 


15m 0.25mm 0.25µm 75–280°C 54570 

0.32mm 0.50µm 75–280°C 54573 

30m 0.25mm 0.25µm 75–280°C 54580 

0.32mm 0.50µm 75–280°C 54583 

60m 0.25mm 0.25µm 75–280°C 54590 

0.32mm 0.50µm 75–280°C 54593 

BPX 608 Capillary Columns 


Temp. Limits* 

min.–max. 

Part No. 

25m 0.32mm 0.4µm 0–360/370°C 54823 


BPX Volatiles Capillary Columns 


Temp. Limits* 

min.–max. 

Part No. 

20m 0.18mm 1µm 0–290/300°C 54978 

30m 0.25mm 1.4µm 0–290/300°C 54980 

0.32mm 1.8µm 0–290/300°C 54982 

0.53mm 3µm 0–290/300°C 54984 

40m 0.18mm 1µm 0–290/300°C 54979 

60m 0.25mm 1.4µm 0–290/300°C 54981 

0.32mm 1.8µm 0–290/300°C 54983 

0.53mm 3µm 0–290/300°C 54985 


SGE ® HT5— 

5% Phenyl (equiv.) 

Polycarborane-siloxane 

• A unique high-temperature phase 

HT5 Polyimide Clad Capillary Columns 


Temp. Limits* 

min.–max. 

Part No. 

15m 0.25mm 0.1μm 10–380/400°C 054633 

0.32mm 0.5μm 10–380/400°C 054667 

0.53mm 0.5μm 10–380/400°C 054671 

30m 0.25mm 0.1μm 10–380/400°C 054634 

0.32mm 0.5μm 10–380/400°C 054668 

0.53mm 0.5μm 10–380/400°C 054672 


HT5 Aluminum Clad Capillary Columns 


Temp. Limits* 

min.–max. 

Part No. 

12m 0.32mm 0.10μm 10–460/480°C 054651 

0.53mm 0.15μm 10–460/480°C 054662 

50m 0.32mm 0.10μm 10–460/480°C 054653 


SGE ® SolGeL Capillary 

Columns 

• Unique technology encapsulates phase in synthetic glass 

• Greater inertness and thermal stability than “coated” 

phases 

SolGeL-WAX Capillary Columns 

• Highest temperature polyethylene glycol column 

• Maximum temperature 300°C 

• Ideal for: essential oils, food additives, industrial solvents, 

unreacted latex monomers, mixtures of aromatic 

hydrocarbons (e.g., BTEX), FAMEs, and mixtures of 

alcohols, esters, aldehydes, and ketones 

SolGeL-WAX is a bonded polyethylene glycol stationary 

phase. It is unique technology because the phase is 

encapsulated in synthetic glass (SolGeL material) and the 

whole matrix is itself bonded to the surface of the fused silica. 

This process leads to a very inert high-temperature column. 

SolGeL-WAX Capillary Columns 


Temp. Limits 

min.–max. 

Part No. 

30m 0.25mm 0.25µm 0–300°C 54706 

0.32mm 0.50µm 0–300°C 54707 

60m 0.25mm 0.25µm 0–300°C 54708 

0.32mm 0.50µm 0–300°C 54709 

SolGeL-1ms Capillary Columns 

• MS-grade capillary columns 

• Maximum temperature 380°C 

• Ideal for: essential oils, pharmaceuticals, food additives, 

industrial solvents, and hydrocarbons 

SolGeL-1ms is a low bleed bonded polydimethylsiloxane 

(100% methyl) stationary phase encapsulated in synthetic 

glass (SolGeL material) and the whole matrix is itself bonded 

to the surface of the fused silica capillary. The anchoring of 

the matrix to the glass surface using this unique technology 

leads to a very inert, high-temperature column. 

SolGeL-1ms Capillary Columns 


Temp. Limits 

min.–max. 

Part No. 

30m 0.25mm 0.25µm 0–380°C 54715 

0.32mm 0.25µm 0–380°C 54718 

60m 0.25mm 0.25µm 0–380°C 54713 

0.32mm 0.25µm 0–380°C 54714 



Alltech ® Custom Packed Metal and PTFE Columns 

Before ordering a custom-packed GC column, consult the list of popular packed columns on pages 244–245 to see if the column 

you need is there. Each column comes complete with brass nuts and brass ferrules. For assistance or a quote, please contact 

Technical Support. 

Coiling Schematics 

A 

A 

B 

C 

D 

C 

B 

D 

A 

C 

B 

D 

2483 2484 

2485 

Figure 1 Figure 2 

Figure 3 

C 

A 

D 

B 

2486 2487 

2488 


Figure 6 

A 

C 

E 

D 

B 

C 

A 

D 

B 

Custom Metal and PTFE Columns — Ordering Instructions 

Specify Coiling 

1) Instrument Make and Model: ______________________ 

2) Configuration Number: (see coiling schematics) ______ 

3) Dimensions: A= ______ in/mm (circle one) 

B= ______ in/mm (circle one) 

C= ______ in/mm (circle one) 

D= ______ in/mm (circle one) 

E= ______ in/mm (circle one) 

F= ______ in/mm (circle one) 

X= ______ in/mm (circle one) 

4) Column Length: ______ ft/m (circle one) 

5) Column i.d.: ______ in/mm (circle one) 

6) Column o.d.: ______ in/mm (circle one) 

7) Tubing Material: (circle one) 

Stainless Steel AT-Steel Nickel PTFE 

TFE-Coated SS Aluminum Copper 

8) Solid Support: ______ Mesh Size: ______ 

Specify Packing 

Choose either a Pretested Packing (p368–369) or create your own custom 

(refer to p370–371) by specifying information below: 

9) Stationary Phase A: ______ % Loading: ______ 

Stationary Phase B*: ______ % Loading: ______ 

Stationary Phase C*: ______ % Loading: ______ 

10) Mirror Image: 

______ Please check if a mirror image is required. 

11) Injection Port Design: (circle one) 

on-column not on-column 

12) Stainless Steel Fittings: 

______ Please check if stainless steel fittings are required 

(PN: C-14010N) 

13) Preconditioning: 

______ Please check if column preconditioning is required 

(PN: C-14005) 

14) Other Special Instructions: (please be specific) 

________________________________________________ 

________________________________________________ 

________________________________________________ 

gc columns | packed 

C 

*Additional charge applies to dual- and triple-coated packings (Part No. C-14020). 

A 

B 

D 

E 

F 

E 

C 

B 

D 

F 

A 

X 

B 

A 

Custom Packed Columns 

Description 

Part No. 

Custom Packed Columns 

C-5000 

(Specify all information from Table 1) 

Empty Columns 

Empty Column with Fittings and Ferrules C-5100 

Additional Charges (per Column) 

Preconditioned Column C-14005 

Stainless Steel Fittings 

C-14010N 

Dual- and Triple-Coated Packings C-14020 

2489 2490 

2491 


Figure 9 

more info 

Certain expensive stationary phases and packings may require a 

surcharge. Be sure to state coiling instructions. 


239


Alltech ® GC Packed Columns 

Custom Deactiglas ® Glass 

• Silane treated for inertness 

• Precision bore glass for reproducibility 

• Brass fittings and vespel/graphite ferrules included 

• Available packed or empty 

Key to Configurations for Glass Columns 

A1 (Arm 1) 

S (Span) 

A2 (Arm 2) 

A1 (Arm 1) 


Alltech ® Deactiglas ® GC Columns are fabricated from 

precision-bore (2mm ±0.05mm or 4mm ±0.1mm i.d.) glass. 

All columns are 1/4" o.d. unless otherwise specified. Columns 

longer than 2 meters may have joints. Several additional 

services such as stainless steel fittings and preconditioning 

of the packing are available. Certain expensive stationary 

phases and packings may require a surcharge. 

Due to space limitations we are not able to list all of the 

different manufacturers columns in our catalog. However, 

they are still available and can be specified at time of order 

placement. If you do not see your instrument on the following 

pages, please call Customer Service. 

Special Services for Glass Columns 

Description 

Part No. 

Stainless Steel Fittings (Per Column) 

C-14010N 

Preconditioned Packing (Per Column) C-14005 

5290 

A1 (Arm 1) 

A2 (Arm 2) 

A2 (Arm 2) 

S (Span) 

S (Span) 

Deactiglas ® Column Ordering Instructions: 

1. Choose column configuration part no. below. 

2. Choose either a pretested packing (p247–248) or create a custom 

packing (refer to p249–250) by specifying information below: 

Stationary Phase A 

% Loading 

Stationary Phase B 

% Loading 

Stationary Phase C 

% Loading 

0043 

Deactiglas ® Glass GC Columns 

Chromatograph Configuration Length i.d. 

Agilent 5830, 5840, 5985 

2513 

Packed 

Part No. 

Empty 

Part No. 

On-Column Injection 

A1 = 10.72" (272mm) 3ft 2mm 131122 131121 

A2 = 7.18" (182mm) 6ft 2mm C6067 6067 

S = 6" (152mm) 6ft 4mm C6076 6076 

4691 

Not On-Column Injection 

A1 = 8.95" (227mm) 3ft 2mm C6564 6564 

A2 = 9.25" (235mm) 6ft 2mm C6568 6568 

S = 6" (152mm) 6ft 4mm C6572 6572 

On-Column Injection 

A1 = 10.72" (272mm) 6ft 2mm C6055 6055 

A2 = 7.18" (182mm) 6ft 4mm C6061 6061 

S = 9" (229mm) 

2514 

Not On-Column Injection 

A1 = 8.95" (227mm) 6ft 2mm C6578 6578 

A2 = 9.25" (235mm) 6ft 4mm C6580 6580 

S = 9" (229mm) 

4695 





Deactiglas ® Glass GC Columns (continued) 


Agilent 5880, 5890, 5987, 6890* 

2515 

Packed 

Part No. 

Empty 

Part No. 

3ft 2mm 130962 130961 

3ft 4mm 130972 130971 

4ft 2mm C6082 6082 

Configuration A 4ft 4mm C6085 6085 

On-Column Injection 6ft 2mm C6087 6087 

A1 = 11.02" (280mm) 6ft 4mm C6089 6089 

A2 = 9.05" (230mm) 2m 2mm C6336 6336 

S = 9" (229mm) 2m 4mm C6343 — 

FID 8ft 2mm C6347 6347 

1.8m 3mm C6346 6346 

10ft 2mm C6091 6091 

10ft 4mm 131552 131551 

4692 

6ft 2mm C6368 6368 

Configuration B 6ft 4mm C6391 6391 

On-Column Injection 2m 2mm C6393 6393 

A1 = 11.02" (280mm) 2m 4mm C6396 6396 

A2 = 7.09" (180mm) 8ft 2mm C6413 6413 

S = 9" (229mm) 1.8m 3mm C6346 6346 

TCD 10ft 2mm C6431 6431 

10ft 4mm C6435 6435 

3ft 2mm C6427 6427 

3ft 4mm C6433 6433 

Configuration C 4ft 2mm C6436 6436 

Not On-Column Injection 4ft 4mm C6440 6440 

A1 and A2 = 9.05" (230mm) 6ft 2mm C6442 6442 

S = 9" (229mm) 6ft 4mm C6445 6445 

All Detectors 2m 2mm C6447 6447 

Use with Liner or 2m 4mm C6449 6449 

Disposable Insert 8ft 2mm C6452 6452 

4693 

2.4m 2mm C6454 6454 

10ft 2mm C6456 6456 

10ft 4mm C6460 6460 

PerkinElmer ® 115, 300, 900, 910, 990, F30, 2000, 2100, 3920, Sigma Series 

2ft 2mm 132442 132441 

3ft 2mm 131432 131431 

3ft 4mm 131442 131441 

1m 2mm C6437 6437 

4ft 2mm C6148 6148 

Not On-Column Injection 4ft 4mm C6150 6150 

A1 = 9" (229mm) 6ft 2mm C6152 6152 

A2 = 9" (229mm) 6ft 4mm C6154 6154 

S = 8.75" (222mm) 2m 2mm C6439 6439 

2m 4mm C6441 6441 

8ft 2mm C6443 6443 

2530 

2.4m 2mm C6446 6446 

10ft 2mm C6105 6105 

12ft 2mm C6448 6448 


2531 

3ft 2mm C6451 6451 

1m 2mm C6453 6453 

On-Column Injection 6ft 2mm C6149 6149 

A1 = 12.62" (321mm) 6ft 4mm C6151 6151 

A2 = 7.09" (180mm) 2m 2mm C6455 6455 

S = 8.75" (222mm) 2m 4mm C6458 6458 

2.4m 2mm C6462 6462 

10ft 2mm C6106 6106 

more info 

For explanation of A1, A2, and S, refer to key on page 240. 


241






PerkinElmer ® 8300, 8400, 8700 Series, 9000 Series, Autosystem 

2534A 

Packed 

Part No. 

Empty 

Part No. 

3ft 2mm C6464 6464 

Not On-Column Injection 1m 2mm C6466 6466 

A1 = 6.75" (171mm) 6ft 2mm C6479 6479 

A2 = 6.75" (171mm) 6ft 4mm C6482 6482 

S = 6.5" (165mm) 2m 2mm C6468 6468 

2m 4mm C6584 6584 

2.4m 2mm C6471 6471 


3ft 2mm C6474 6474 

On-Column Injection 1m 2mm C6477 6477 

A1 = 12" (305mm) 4ft 2mm C6243 6243 

A2 = 6.75" (171mm) 4ft 4mm C6245 6245 

S = 6.5" (165mm) 6ft 2mm C6247 6247 

6ft 4mm C6249 6249 

2534B 

2m 2mm C6484 6484 

Shimadzu ® 4BF, 4BM, 4CM, 4MG, 6A, 6AM, 7AG (No Fittings Included) 

A1 = 13.2" (335mm) 1.7m 3mm C6509 6509 

A2 = 11.1" (282mm) 2m 2mm* C6349 6349 

S = 1.57" (40mm) 2m 3mm C6485 6485 

2537 

5mm o.d. Tubing 2m 4mm* C6359 6359 

2.5m 3mm C6515 6515 

Shimadzu ® 8A, RIA, GC-8A8IF (No Fittings Included) 

1m 3mm C6489 6489 

A1 = 9" (229mm) 1.5m 2.6mm C6598 6598 

A2 = 9" (229mm) 1.5m 3mm C6658 6658 

S = 6" (152mm) 2m 2mm* C6281 62811 

5mm o.d. Tubing 2m 2.6mm C6673 6673 

2m 3mm C6493 6493 

2539 

2m 4mm* C6334 6334 

Shimadzu ® 7A, 9A, 12A, 14A, 14B, 15A, 16A (No Fittings Included) 

1m 2.6mm C6487 6487 

1m 3mm C6497 6497 

1.7m 2.6mm C6491 6491 

A1 = 10.69" (272mm) 1.7m 3mm C6495 6495 

A2 = 12.81" (327mm) 2m 2mm* C6337 6337 

S = 1.57" (40mm) 2m 2.6mm C6498 6498 

2538 

5mm o.d. Tubing 2m 3mm C6499 6499 

2m 4mm* C6367 6367 

2.4m 2.6mm C6501 6501 

2.4m 3mm C6505 6505 

Varian ® 3300, 3400 

3ft 2mm C6558 6558 

Injector B to Detector B 4ft 2mm C6261 6261 

A1 = 9.5" (241mm) 4ft 4mm C6263 6263 

A2 = 8.1" (206mm) 6ft 2mm C6265 6265 

S = 6.82" (173mm) 6ft 4mm C6283 6283 

FID 2m 2mm C6340 6340 

2549 

2m 4mm C6342 6342 

*1/4" o.d. Column tapered to 5mm o.d. x 2.6mm i.d. ends. 

more info 

For explanation of A1, A2, and S, refer to page 240. 







Varian ® 3300, 3400,3600, 3700, 4400, 4600, 6000 Vista Series 

Packed 

Part No. 

Empty 

Part No. 

3ft 2mm 131662 131661 

3ft 4mm 131672 131671 

1m 2mm C6306 6306 

1m 4mm C6308 6308 

Injector A to Detector A 4ft 2mm C6189 6189 

A1 = 9.5" (241mm) 4ft 4mm C6190 6190 

A2 = 8.1" (206mm) 6ft 2mm C6191 6191 

S = 5.5" (140mm) 6ft 4mm C6192 6192 

FID 2m 2mm C6310 6310 

0050A 

2m 4mm C6312 6312 

2.4m 2mm C6560 6560 

10ft 2mm C6197 6197 

10ft 4mm 131692 131691 

4m 2mm C6314 6314 

4m 4mm C6316 6316 

Varian ® Universal Columns 3300, 3400, 3600, 3700, 4400, 4600, 6000 Vista Series 

3ft 2mm C6562 6562 

6ft 2mm C6206 6206 

A1 = 9.5" (241mm) 6ft 4mm C6208 6208 

A2 = 2.25" (57mm) 2m 2mm C6356 6356 

0050C 

S = N/A 2m 4mm C6358 6358 

FID, TCD, ECD 4m 2mm C6360 6360 

4m 4mm C6366 6366 

more info 

For explanation of A1, A2, and S, refer to page 240. 

Adapter Kit 

Description 

Part No. 

Universal Adapter Kit 6210 


more info 

Universal Glass Column Adapters are available for the Varian ® 

3000 and Varian ® 3700/Vista Series. Please contact Customer 

Service for part numbers and prices. 

Hi-EFF Pre-Columns 

• Extend column life 

• Reduce sample clean-up 

• Perform reaction chromatography 

• Perform subtraction chromatography 

To order a Hi-EFF Pre-Column, state catalog number and, 

if packed, describe the packing (% load, phase, support, and 

mesh). Glass and glass-lined stainless steel and columns are 

deactivated at no charge. 

Deactiglas ® Column Inlet 

Liners 

• Prolong the life of your glass columns 

• Silane-treated for inertness 

• Available for 2mm or 4mm i.d. columns 

• Inserts directly into the end of your column 

Deactiglas ® 

Column 

Inlet Liner 

2mm i.d. Column 

4mm i.d. Column 

Glass Wool 

Column Packing 

Hi-EFF Pre-Columns 

Packed 

Part No. 

Empty 

Part No. 

Description Length Qty. 

1/8" SS 6" 6 5900 5910 

1/4" Glass with Guide 6" 6 5902 5912 

1/8" Glass-Lined SS 6" 3 5904 5914 

1/8" Bottom-Drilled SS Union — ea — 5920 

1/4" Bottom-Drilled SS Union — ea — 5921 

Deactiglas ® Column Inlet Liners 


For 2mm i.d. Columns 10 6301 




Glass Wool Inserter/Remover 2 6292 


243


Alltech ® Popular GC Packed Columns 

These columns are 6' x 1/8" o.d. 304 premium grade, 

precleaned stainless steel (unless otherwise indicated). 

Each column is made with preconditioned, pretested packing 

and comes complete with fittings, packing quality control 

chromatogram, column tags, and conditioning instructions. 

Popular Packed Column Ordering Instructions: 

1. Choose the part no. for your column from pages 244–245. 

2. Specify make and model of GC.* 

3. If your column is not listed, see page 239 for custom columns. 

*If no coiling instructions are provided, we will supply in a nominal 6" coil. 


Popular GC Packed Columns 

Packing Description Dimensions* Part No. 

Alumina F-1, 60/80 5' x 1/8" SS Tubing 5664PC 

1% AT-1000 on Carbograph 1, 60/80 14404 

1% AT-1000 on Carbograph 1, 60/80 24' x 1/8" SS Tubing 14468 

10% AT-1000 on Chromosorb ® W-AW, 80/100 12001PC 

0.10% AT-1000 on Graphpac-GC, 80/100 8542PC 

10% Carbowax ® 1500 on Chromosorb ® T, 40/60 14479 

6.6% Carbowax ® 20M on Carbograph 1 AW, 80/100 10' x 1/8" SS Tubing 14473 

5.0% Carbowax ® 20M on Chromosorb ® W-HP, 80/100 14482 

10% Carbowax ® 20M on Chromosorb ® W-AW, 80/100 12005PC 

10% Carbowax ® 20M on Chromosorb ® W-HP, 80/100 12106PC 

6.6% Carbowax ® 20M on Graphpac-GB, 80/120 10' x 1/8" SS Tubing 14472 

0.20% Carbowax ® 1500 on Graphpac-GC, 80/100 8546PC 

Carbosphere, 60/80 

5680PC 

Carbosphere, 80/100 10' x 1/8" SS Tubing 56821PC 

Carbosphere, 80/100 

5682PC 

Chemipack C18, 80/100 

2768PC 

Chromosorb ® 101, 80/100 

2406PC 


2409PC 


2424PC 

Chromosorb ® 107, 80/100 6' x 1/8" TFE Tubing 9783 

Chromosorb ® 750, 80/100 4' x 1/8" SS Tubing 14480 

30% DC-200 (500cstks) on Chromosorb ® P-AW, 60/80 14481 

30% DC-200 (500cstks) on Chromosorb ® P-AW, 60/80 30' x 1/8" SS Tubing 12713PC 

10% FFAP on Chromosorb ® W-AW, 80/100 8434PC 

Gas Chrom 220, 80/100 

2484PC 

Gas Chrom 254, 80/100 

2486PC 

Gas Chrom MP-1, 100/120 30' x 1/8" SS Tubing 4945PC 

HayeSep ® A, 80/100 14484 

HayeSep ® A, 120/140 40' x 1/8" SS Tubing 28211 

HayeSep ® B, 80/100 14485 

HayeSep ® D, 80/100 14487 

HayeSep ® D, 100/120 3' x 1/8" SS Tubing 14486 

HayeSep ® D, 100/120 20' x 1/8" Ni Tubing 27082PC 

HayeSep ® D, 100/120 30' x 1/8" SS Tubing 27083PC 

HayeSep ® D, 100/120 10' x 1/8" SS Tubing 28301PC 

HayeSep ® DB, 100/120 30' x 1/8" SS Tubing 2836PC 

HayeSep ® Dip, 100/120 9' x 1/8" SS Tubing 2833PC 

HayeSep ® N, 80/100 14488 

HayeSep ® Q, 60/80 14490 

HayeSep ® Q, 80/100 8' x 1/8" SS Tubing 28010PC 

HayeSep ® Q, 80/100 

2801PC 

HayeSep ® Q, 100/120 14489 

HayeSep ® P, 60/80 8' x 1/8" SS Tubing 2803PC 

HayeSep ® P, 80/100 

2804PC 

HayeSep ® R, 80/100 

2807PC 

HayeSep ® S, 100/120 

2811PC 

HayeSep ® T, 60/80 3' x 1/8" SS Tubing 14491 

HayeSep ® T, 80/100 

2813PC 

HayeSep ® T, 100/120 5' x 1/8" SS Tubing 2814PC 

*6' x 1/8" stainless steel unless otherwise specified. 



Alltech ® Popular GC Packed Columns (continued) 

Popular GC Packed Columns** (continued) 

Packing Description Dimensions* Part No. 

5% Krytox ® 143 AC on Graphpac-GB 60/80 10' x 1/8" SS Tubing 85222 

Molecular Sieve 5A (Washed), 60/80 14494 

Molecular Sieve 5A (Washed), 80/100 

5605PC 

Molecular Sieve 5A (Washed), 80/100 10' x 1/8" SS Tubing 14495 

Molecular Sieve 13X (Washed), 60/80 5' x 1/8" SS Tubing 57730PC 

Molecular Sieve 13X (Washed), 80/100 3' x 1/8" SS Tubing 14492 

Molecular Sieve 13X (Washed), 80/100 

5773PC 

Molecular Sieve 13X (Washed), 80/100 10' x 1/8" SS Tubing 14493 

3% OV-1 on Chromosorb ® W-HP, 80/100 12716PC 

10% OV-101 on Chromosorb ® P-AW, 80/100 4' x 1/8" SS Tubing 14477 

10% OV-101 on Chromosorb ® P-AW, 80/100 14478 

20% OV-101 on Chromosorb ® P-AW, 80/100 14469 



20% OV-101 on Chromosorb ® W-HP, 80/100 14470 




0.19% Picric Acid on Graphpac-GC, 80/100 7' x 1/8" SS Tubing 85501PC 

Porapak ® N, 80/100 3' x 1/8" SS Tubing 14497 

Porapak ® N, 80/100 

2716PC 



Porapak ® P, 80/100 14499 

Porapak ® PS, 80/100 10' x 1/8" SS Tubing 14506 

Porapak ® PS, 100/120 3' x 1/8" SS Tubing 14500 

Porapak ® Q, 50/80 4' x 1/8" SS Tubing 14508 

Porapak ® Q, 50/80 

2700PC 

Porapak ® Q, 80/100 3' x 1/8" SS Tubing 14519 

Porapak ® Q, 80/100 12' x 1/8" SS Tubing 27012PC 

Porapak ® Q, 80/100 

2701PC 

Porapak ® Q, 100/120 

2702PC 

Porapak ® QS, 80/100 

2719PC 

Porapak ® R, 80/100 

2707PC 

Porapak ® S, 50/80 7' x 1/8" SS Tubing 2709PC 

Porapak ® S, 80/100 

2710PC 

Porapak ® T, 80/100 

2713PC 

5% SE-30 on Chromosorb ® W-HP, 80/100 12025PC 

10% SE-30 on Chromosorb ® W-HP, 80/100 12423PC 

Silica Gel Grade 12, 60/80 8' x 1/8" SS Tubing 14523 

Silica Gel Grade 12, 60/80 18' x 1/8" SS Tubing 5651PC 

Silica Gel Grade 12, 80/100 8' x 1/8" SS Tubing 14530 

10% Silar 10C on Chromosorb ® W-HP, 100/120 12430PC 

20% Squalane on Chromosorb ® P-AW, 80/100 3' x 1/8" SS Tubing 14471 

15% Squalane on Chromosorb ® P-AW-DMCS, 80/100 8' x 1/8" SS Tubing 5327PC 

Sulfur Gas Column 36' x 1/8" TFE Tubing 9779 

18" of Super Q, 80/100 30" x 1/8" TFE Tubing 9781 

Tenax ® TA, 60/80 4' x 1/8" SS Tubing 14538 

Tenax ® TA, 60/80 

4900PC 

Tenax ® TA, 80/100 

4901PC 

Tenax ® GR, 60/80 

4937PC 

Unibeads 1S, 60/80 

2752PC 

Unibeads 2S, 60/80 

2760PC 

Unibeads 3S, 60/80 14541 

Unibeads 3S, 80/100 14542 

VZN-1, 60/80 23' x 1/8" SS Tubing 84623PC 

10% Silicone UCW-98 on Chromosorb ® W-HP, 80/100 8493PC 

1% TCEP on Carbograph 1, 80/120 4' x 1/8" SS Tubing 14474 

*6' x 1/8" stainless steel unless otherwise specified. **To order, use the part number shown and specify the instrument for which it should be coiled. 



245


Alltech ® CTR Concentric Packed Columns 

For Gas Analysis 

• CTR I is ideal for separating O2, N2, CH4, CO, CO2 at 

ambient conditions 

• CTR III is ideal for argon analysis in the presence of O2 

• Useful where two separate chromatographic runs are 

required to analyze one sample 

• Individually tested 

A CTR column is essentially a column within a column. 

This permits you to use two different packings for the analysis 

of your sample. The diagram shows the construction of the 

CTR column. 

Inner 1/8" o.d. Tube Containing 

Column Packing A 

Cut-away view of CTR column. 

Outer 1/4" o.d. Tube Containing 

Column Packing B 

3980 


CTR I—Oxygen, Nitrogen, Methane, Carbon 

Monoxide, and Carbon Dioxide 

Until the CTR, no single column operating at room 

temperature has been designed to permit, in one analysis, all 

these components in air. The analysis is usually accomplished 

by separate runs or with expensive valves. The CTR I 

accomplishes this separation in less than nine minutes. 

Calibration Gas for CTR I Column—This special mixture is 

useful for calibrating Part No. 8700 CTR I columns. It contains 

approximately 15% CO2 + 7% CO + 7% O2 + 4.5% CH4 with 

the balance N2. It is supplied in a 4L push button can with a 

needle applicator. 

CTR III Argon Analysis Column 

The CTR III is special designed for the separation of argon in 

the presence of oxygen. The outer column contains a special 

adsorbent that removes oxygen allowing the elution of argon 

and nitrogen. The inner column allows the combined elution 

of a peak containing oxygen and argon and later nitrogen. 

This separation is accomplished at ambient temperatures 

in ten minutes. 

more info 

For GC Applications, see the GC Applications Section, pages 

462–493. 

CTR I Column 

Description 

Temp. Limit 

max. 

Part No. 

CTR I Column 275°C 8700 

Calibration Gas for CTR I — 9799 

CTR III Column 

Description 

Temp. Limit 

max. 

Part No. 

CTR III Argon Analysis Column 275°C 8725 

Additional Fittings 

Tube End Connector, 1/4" to 1/8" Tube — 14169 

CTR I — Air, CO, CH4, CO2, O2, N2 

1059 

CTR III — Argon in Air 

1060 

1 3 

2 

4 

5 

1. Air and Carbon Monoxide 

(Inner Column) 

2. Methane (Inner Column) 

3. Carbon Dioxide (Inner Column) 

4. Oxygen (Outer Column) 

5. Nitrogen (Outer Column) 

6. Methane (Outer Column) 

7. Carbon Monoxide (Outer Column) 

1 

2 3 4 1. Argon (Inner Column) 

2. Nitrogen (Inner Column) 

3. Oxygen + Argon (Outer 

Column) 


6 7 

0 1 2 3 4 5 6 7 8 9 Min. 

Column: CTR I (Part No. 8700) 

Outer Column: 6ft x 1/4" Packed with Activated Molecular Sieve 

Inner Column: 6ft x 1/8" Packed with Porous Polymer Mixture 

Temp: 

Ambient 

Carrier Gas: Helium at 65mL/min 

Detector: TCD 

0 2 4 6 8 10 12 Min. 

Column: CTR III, 6ft x 1/4" SS (Part No. 8725) 


Inner Column: 6ft x 1/8" Packed with Molecular Sieve and 

Oxy Adsorbent 

Temp: 

Ambient 


Detector: TCD 



Pretested GC Packings 

Pretested packings are guaranteed and supplied with a test 

chromatogram. Preconditioning service is available for 20g 

or 25g of packing. The packing is heated at 25°C below the 

upper temperature limit for 3 hours with a flow of oxygen-free 

nitrogen. 

Preconditioning Services 

Description 

Packing Preconditioning Service (per 20–25 grams). 

Specify Pretested GC Packing Part Number. 

Part No. 

C-14000 

Pretested GC Packings 

Packing Description Temp. Limits Qty. Part No. 

Alumina F-1, 60/80 300°C 100g 5664 

1% AT-1000 on Carbograph 1, 60/80 250°C 15g 1733 

10% AT-1000 on Chromosorb ® W-AW, 80/100 250°C 20G 12001 

0.10% AT-1000 on Graphpac-GC, 80/100 225°C 15g 8542 

10% Carbowax ® 1500 on Chromosorb ® T, 40/60 175°C 20g 16146 

6.6% Carbowax ® 20M on Carbograph 1-AW, 80/120 60–225°C 15g 1731 

5.0% Carbowax ® 20M on Chromosorb ® W-HP, 80/100 60–225°C 20g 16147 

5.0% Carbowax ® 20M on Chromosorb ® T, 40/60 60–225°C 25g 8416 

10.0% Carbowax ® 20M on Chromosorb ® W-AW, 80/100 60–225°C 20g 12005 

10.0% Carbowax ® 20M on Chromosorb ® W-HP, 80/100 60–225°C 20g 12106 

4% Carbowax ® 20M + 0.8% KOH on Graphpac-GB, 60/80 220°C 15g 8519 

6.6% Carbowax ® 20m on Graphpac-GB, 80/120 175°C 15g 16148 

0.2% Carbowax ® 1500 on Graphpac-GC, 80/100 175°C 15g 8546 

Carbosphere ® , 60/80 400°C 5g 5680 

Carbosphere ® , 80/100 400°C 5g 5682 

Chemipack C18, 80/100 320°C 15mL 2768 

Chromosorb ® 101, 80/100 275/325°C 25g 16149 

Chromosorb ® 102, 80/100 250/300°C 25g 16150 

Chromosorb ® 107, 80/100 250/275°C 25g 16151 

Chromosorb ® 750, 80/100 250/275°C 25g 16152 

30% DC-200 (500 cstk) on Chromosorb ® P-AW, 60/80 20–200°C 25g 12713 

10% FFAP on Chromosorb ® W-AW, 80/100 50–250°C 25g 8434 

28% AT-223 + 4% KOH on Gas Chrom R, 80/100 200°C 25g 49259 

Gas Chrom 220, 80/100 250°C 25g 2484 

Gas Chrom 254, 80/100 275°C 25g 2486 

Gas Chrom MP-1, 100/120 50–250°C 25g 16153 

HayeSep ® A, 80/100 165°C 15g 16154 

HayeSep ® A, 120/140 165°C 15g 16155 

HayeSep ® B, 80/100 190°C 15g 16156 

HayeSep ® D, 80/100 290°C 15g 16157 



247



Pretested GC Packings (continued) 

Pretested GC Packings (continued) 

Packing Description Temp. Limits Qty. Part No. 

HayeSep ® D, 100/120 290°C 15g 16166 

HayeSep ® DB, 100/120 290°C 15g 16169 

HayeSep ® Dip, 100/120 290°C 15g 16170 

HayeSep ® N, 80/100 165°C 15g 16172 

HayeSep ® Q, 60/80 275°C 15g 16196 

HayeSep ® Q, 80/100 275°C 15g 16197 

HayeSep ® Q, 100/120 275°C 15g 16198 

HayeSep ® P, 60/80 250°C 15g 16199 

HayeSep ® P, 80/100 250°C 15g 16210 

HayeSep ® R, 80/100 250°C 15g 16211 

HayeSep ® S, 100/120 250°C 15g 16212 

HayeSep ® T, 60/80 165°C 15g 16213 

HayeSep ® T, 80/100 165°C 15g 16214 

HayeSep ® T, 100/120 165°C 15g 16215 

5% Krytox ® 143AC on Graphpac GB, 60/80 175°C 15g 8522 

Molecular Sieve 5A (washed), 60/80 350°C 50g 5604 

Molecular Sieve 5A (washed), 80/100 350°C 50g 5605 

Molecular Sieve 13X (washed), 60/80 350°C 50g 57730 

Molecular Sieve 13X (washed), 80/100 350°C 50g 57732 

3% OV-1 on Chromosorb ® W-HP, 80/100 100–350°C 20g 12716 

10% OV-101 on Chromosorb ® P-AW, 80/100 20–350°C 20g 16216 








0.19% Picric Acid on Graphpac-GC, 80/100 120°C 15g 8550 

Porapak ® N, 80/100 190°C 15g 16219 

Porapak ® N, 100/120 190°C 15g 16220 

Porapak ® P, 80/100 250°C 15g 16221 

Porapak ® PS, 80/100 250°C 15g 16223 

Porapak ® PS, 100/120 250°C 15g 16224 

Porapak ® Q. 50/80 250°C 15g 16225 

Porapak ® Q, 80/100 250°C 15g 16226 

Porapak ® Q, 100/120 250°C 15g 16228 

Porapak ® QS, 80/100 250°C 15g 16229 

Porapak ® R, 80/100 250°C 15g 16230 

Porapak ® S, 50/80 250°C 15g 16231 

Porapak ® S, 80/100 250°C 15g 16232 

Porapak ® T, 80/100 190°C 15g 16233 

Silica Gel Grade 12, 60/80 350°C 100g 5651 

Silica Gel Grade 12, 80/100 350°C 100g 5653 

20% Squalane on Chromosorb ® P-AW, 80/100 140°C 20g 16235 

15% Squalane on Chromosorb ® P-AW-DCMS, 80/100 140°C 20g 16236 

Tenax ® TA, 60/80 350°C 10g 04916 

Tenax ® TA, 80/100 350°C 10g 04917 

Tenax ® GR, 60/80 350°C 10g 4937 

Unibeads 1S, 60/80 200°C 25g 2752 

Unibeads 2S, 60/80 200°C 25g 2760 

Unibeads 3S, 60/80 200°C 25g 2764 

Unibeads 3S, 80/100 200°C 25g 2766 

VZN-1, 60/80 50°C 10g 8462 

1% TCEP on Carbograph 1, 80/120 180°C 15g 16270 



Custom Coated Packings 

Before ordering a custom coated packing, please check the 

list of stock pretested packings on pages 247–248 (called 

"Pretested" on these pages). You may find the packing you 

need in stock and at a savings. 

Custom Coated Packing Ordering Instructions: 

1. Choose your solid support from Table 1. 

2. Specify mesh range. 

3. Choose the stationary phase from Table 3 or 4. It is possible to coat 

up to three stationary phases on one support). 

4. Specify % loading for each stationary phase. Note maximum % 

loading guidelines for each support in Table 2. 

Table 1—Base Price for Custom-Coated Packings 

20 Grams 

Part No. 

50 Grams 

Part No. 

100 Grams 

Part No. 

Solid Support 

Chromosorb ® G, W (HP) C-3004 C-3036 C-3068 

Chromosorb ® G, W, or P (NAW, AW, DMCS) C-3006 C-3038 C-3070 

Chromosorb ® T C-3008 C-3040 C-3072 

Chromosorb ® 101-108 C-2031 C-2032 — 

Chromosorb ® 750 C-3012 C-3044 C-3076 

Gas Chrom S C-3018 C-3050 C-3082 

Gas Chrom R C-3020 C-3052 C-3084 

Gas Chrom 220, 254 C-3022 C-3054 C-3086 

Graphpac-GC and -GB (15 grams), Carbograph C-2200 — — 

Porapak ® C-2041 C-2042 — 

Tenax ® or HayeSep ® (15 grams) C-3028 — — 

Table 2—Maximum Stationary Phase Loading for Solid Supports 

Max. Load Supported Material Max. Load Supported Material Max. Load Supported Material 

5% Chromosorb ® G 12% Chromosorb ® 750 2% Porapak ® 

15% Chromosorb ® W 15% Gas Chrom S, A, P, Z, and Q 2% Super Q 

25% Chromosorb ® A 30% Gas Chrom R 12% T-Port-F 

30% Chromosorb ® P 2% Gas Chrom 220 and 254 2% HayeSep ® 

12% Chromosorb ® T 0.5% Glass Beads 2% Tenax ® 

2% Chromosorb ® 101–108 8% Graphpac, Carbograph 15% Supelcoport 


Stationary Phases 

Table 3—GC Stationary Phases 

Description 

Solvent 

Temp. Limits 

min.–max. USP Code Qty. Part No. 

Apiezon ® L Chloroform 50–300ºC — 25g 5017 

bis-(2-Ethoxyethyl) Adipate (BEEA) Chloroform 150ºC — 25g 5046 

Carbowax ® 400 Chloroform 20–100ºC G20 100g 5051 

Carbowax ® 20M Chloroform 60–225ºC G16 10g 5264 

DC-200, 350 cstk (Methyl) Chloroform 20–250ºC — 100g 5251 

Dexsil ® 300GC Chloroform 50–400ºC G33 1g 5092 

FFAP Chloroform 50–250ºC G35 20g 5156 

Hi-EFF-1BP (Diethyleneglycol Succinate) 1 Chloroform 20–200ºC G4 25g 09505 

Igepal ® CO-630 Methanol 30–200ºC — 50g 5175 

OV-1 (Methyl Gum) Chloroform 100–350ºC G2 10g 5293 

OV-17 (50% Phenyl) Acetone 20–350ºC G3 20g 5301 

OV-101 (Methyl Fluid) Chloroform 20–350ºC G1 20g 5309 

OV-275 (Dicyanoallyl) Chloroform 250ºC+ — 5g 5314 

QF-1 (50% Trifluoropropyl) Ethyl Acetate 20–250ºC — 25g 5269 

SE-30 (Methyl Gum) Chloroform 75–300ºC — 100g 5271 

Sebaconitrile Chloroform 150ºC — 20g 5244 

Silar 10C (100% Cyanopropyl Silicone) 2 Chloroform 50–250ºC G5 5g 5250 

Tetrahydroxyethylethylene Diamine (THEED) Methanol 125ºC — 25g 5347 

1 

U.S. Patent #3,239,997, 2 U.S. Patent #4,063,911. 


249


Custom Coated Packings 

Additional Stationary Phases for Custom Packings and Columns 


Table 4—Stationary Phases 

AT-1000 bis-(2-Ethoxyethyl) Sebacate (BEES) OV-351 

Apiezon ® H Glycerin Poly-A ® 103 

Apiezon ® J Halocarbon 25-55 Grease Poly-A ® 135 

Apiezon ® K Halocarbon Oil 14-25 Poly-I 110 

Apiezon ® N Hexadecanol Poly-S 176 

BBBT Hi-EFF 1AP (Diethyleneglycol Adipate) Polyox 600M 

Bentone 34 Hi-EFF 2AP (Ethyleneglycol Adipate) Polyvinylpyrrolidone 

BHXBT Hi-EFF 2BP (Ethyleneglycol Succinate) Reoplex 400 

BMBT Hi-EFF 3BP (Neopentylglycol Succinate) SE-52 

BMEA Hi-EFF 3CP (Neopentylglycol Sebacate) SF-96 

Carbowax ® 540 Hi-EFF 8AP (Cyclohexanedimethanol Adipate) Sorbitol 

Carbowax ® 600 Igepal ® CO-880 SPAN 80 

Carbowax ® 1000 Igepal ® CO-990 Squalane 

Carbowax ® 1450 Kel-F ® Oil No. 3 SUPEROX ® 4 

Carbowax ® 20M-TPA Kel-F ® Oil No. 10 SUPEROX ® 20M 

CS-10 Kel-F ® Wax TCEPE 

N,N-bis-(2-Cyanoethyl) formamide (BCEF) Krytox ® 143AD TCEP 

DC-200 (500cstk) LAC 3-R-728 β,β-Thiodipropionitrile 

DC-410 LAC 4-R-886 Triton ® X-100 

DC-550 LAC 5-R-737 Triton ® X-305 

DC-702 Lexan (Polycarbonate Resin) TWEEN 80 

Di-n-butyl Maleate Neopentylglycol Succinate UC L-45 

Didecyl Phthalate Octyldecyl Adipate UCON 50-HB-280-X 

Di(2-ethylhexyl) Sebacate OV-7 UCON 75-H-90,000 

Di(2-ethylhexyl) Phthalate OV-17 Vinyl UCON LB-135 

Diglycerol OV-22 UCON LB-1715 

Diisodecyl Phthalate OV-105 UCON LB-550X 

Dimethyl Sulfolane OV-202 UCON LB-1800-X 

Dinonyl Phthalate OV-210 UC W-98 

ECNSS-M OV-215 Versamid ® 900 

EGSS-X OV-225 Versilube ® F-50 

Ethofat 60-25 OV-330 Versilube ® F-50 

Additional Charges for Custom Coated Packings 

Description 

Multiple Stationary Phases (per phase) 

Expensive Stationary Phases: Add the cost of the 

stationary phase used to base price 

Part No. 

C-2000 

— 

Acetone Washing C-2700 


Need help selecting the right packing for your analysis? 





Looking for GC packing accessories? 


5079 

5075 



GC Supports 

Chromosorb ® Diatomite Supports 

Chromosorb ® A, G, P, and W are available in both 

untreated and treated forms, as well as in a choice of 

various mesh ranges. 

Chromosorb ® Specifications 

Chromosorb ® 

Series 

Surface 

Area 

Packed 

Density 

Type 

A Flux-calcined 2.7m 2 /g 0.48g/cc 25% 

G Flux-calcined 0.5m 2 /g 0.58g/cc 5% 

P Calcined 4.0m 2 /g 0.47g/cc 30% 

W Flux-calcined 1.0m 2 /g 0.24g/cc 15% 

750 Flux-calcined 0.75m 2 /g 0.40g/cc 12% 

Recommended 

Liquid Phase 

Load 

Chromosorb ® Treatment Specifications 

Abbreviation Treatments 

NAW 

Untreated (non-acid washed) 

AW 

Acid washed (HCL is used) 

DMCS 

Dimethyldichlorosilane (silanized) 

HP 

High performance QC’ed (acid washed, silanized, 

flux-calcined) 

Chromosorb ® Diatomite Supports 


Chromosorb ® A-NAW 

20/30 454g 2431 

45/60 454g 2432 

60/80 454g 2433 

Chromosorb ® G-NAW 

45/60 225g 23721 

60/80 225g 23731 

80/100 225g 23741 

100/120 225g 23751 

120/140 225g 23761 

Chromosorb ® G-AW 

45/60 225g 23821 

60/80 225g 23831 

80/100 225g 23841 

100/120 225g 23851 

Chromosorb ® G-AW-DMCS 

45/60 225g 23921 

60/80 225g 23931 

80/100 225g 23941 

100/120 225g 23951 

120/140 225g 23961 

Chromosorb ® G-HP 

60/80 225g 2396 

80/100 225g 2397 

100/120 225g 2398 

120/140 225g 2444 

Chromosorb ® Diatomite Supports (continued) 


Chromosorb ® P-NAW 

30/60 454g 2304 

45/60 454g 2305 

60/80 454g 2306 

80/100 454g 2307 

100/120 454g 2308 

Chromosorb ® P-AW 

30/60 454g 2314 

45/60 454g 2315 

60/80 454g 2316 

80/100 454g 2317 

100/120 454g 2318 

Chromosorb ® P-AW-DMCS 

45/60 454g 2323 

60/80 454g 2324 

80/100 454g 2325 

100/120 454g 2326 

Chromosorb ® W-NAW 

30/60 150g 23331 

45/60 150g 23341 

60/80 150g 23351 

80/100 150g 23361 

100/120 150g 23371 

120/140 150g 23381 

Chromosorb ® W-AW 

30/60 150g 23451 

45/60 150g 23461 

60/80 150g 23471 

80/100 150g 23481 

100/120 150g 23491 

Chromosorb ® W-AW-DMCS 

45/60 150g 23581 

60/80 150g 23591 

80/100 150g 23601 

100/120 150g 23611 

Chromosorb ® W-HP 

60/80 150g 24401 

80/100 150g 23651 

100/120 150g 23661 

120/140 150g 24421 

Chromosorb ® 750 

• Designed specifically for biomedical and pesticide 

analyses 

Chromosorb ® 750 is the most inert and highly efficient support 

material in the series. This support material is acid-washed, 

DMCS treated, and carefully screened. 

Chromosorb ® 750 


60/80 100g 2399 

80/100 100g 2400 

100/120 100g 2401 



251


GC Supports and Packings 

Chromosorb ® T 

Chromosorb ® T is a support material made from PTFE and 

is useful for the analysis of polar compounds such as water, 

hydrazine, sulfur dioxide, etc. 

Chromosorb ® T melts at 327°C, starts to decompose at 

290°C, and fuses together under prolonged use at 250°C. 

The density is 0.42g/cc. The surface area is 7–8m 2 /g and 

maximum loading capacity is 12%. When packing, chill the 

material below 19°C. 

Gas Chrom 

Gas Chrom S is white diatomaceous earth that is screened 

and is non-acid washed. Use as a filtering agent or trapping 

material. 

Gas Chrom R is red diatomaceous earth that is screened 

and water washed to neutrality. 

Gas Chrom Porous Polymers 220 and 254 are low in 

residual monomers and contaminants, condition faster and 

have lower column bleed. 

Chromosorb ® T 


30/60 225g 24371 

40/60 225g 24381 

Gas Chrom Specifications 

Gas Chrom 254 Gas Chrom 220 

Pore Size: 3000nm 10nm 

Surface Area: 1000m 2 /g 450m 2 /g 

Equivalents: Porapak ® P Porapak ® Q 


T-Port-F 

T-Port-F is a screened tetrafluoroethylene support useful for 

analysis of very polar compounds. It has a density of about 

0.5g/cc with a maximum temperature limit of 150°C. 

T-Port-F 


30/60 50g 2447 

60/80 50g 2448 

80/100 50g 2449 

Gas Chrom 


Gas Chrom S 

80/100 100g 03010 

Gas Chrom R 

40/60 100g 4956 

60/80 100g 4966 

80/100 100g 4976 

Gas Chrom Porous Polymer 

220, 80/100 25g 2484 

254, 80/100 25g 2486 

Stock Packed Columns 

6ft x 1/8" SS Column with Gas Chrom Porous ea 2484PC 

Polymer 220, 80/100 

6ft x 1/8" SS Column with Gas Chrom Porous 

Polymer 254, 80/100 

ea 2486PC 

Activated Alumina 

Activated alumina is useful for the analysis of light 

hydrocarbons. Unsaturated hydrocarbons are retained longer 

than saturated ones. Grade F-6 is an indicating alumina 

which turns from blue to pink upon adsorption of water. The 

maximum temperature limit is 300°C. 

Glass Beads 

Glass beads can be loaded up to 0.5 wt. % before becoming 

tacky. Two types are available: untreated (regular) or 

dimethyldichlorosilane (DMCS)-treated. 

Activated Alumina 


Grade F-1 

40/60 100g 5662 

60/80 100g 5664 

80/100 100g 5666 

100/120 100g 5668 

Grade F-6 

8/14 100g 05561 

Stock Packed Column 

5ft x 1/8" SS Packed with Alumina Grade F-1, 

60/80 

ea 5664PC 

Glass Beads 


Regular, 60/80 mesh 125g 5420 

Regular, 80/100 mesh 125g 5422 

Regular, 100/120 mesh 125g 5424 

DMCS Treated, 60/80 mesh 125g 5426 





GC Packings 

Silica Gel 

Silica Gel is used for the analysis of fixed gases and light 

hydrocarbons. It is also useful for dehydration of gases 

and liquids. 

Grade 42 Indicating Silica Gel changes from blue to pink as 

it becomes saturated with water. It conforms to MIL-D-3716 

Type IV Grade H. It will absorb water up to about 40% by 

weight. The maximum temperature limit is 350°C. 

Silica Gel 

Unibeads S 

100g 

Part No. 

1kg 

Part No. 

Description 

Grade 12 

40/60 5650 05532 

60/80 5651 05533 

80/100 5653 05534 

100/120 5655 05535 

Grade 15 

35/60 5648 05545 

Indicating Silica Gel, Grade 42 

6/16 05559 05560 


18ft x 1/8" SS Packed with Silica Gel, 60/80, ea 

5651PC 

The Unibeads S series consists of spherical porous 

silica beads. Unibeads 2S are comparable to Porasil ® A. 

Unibeads 3S are similar to Porasil ® B. The smaller the pore 

size, the greater the retention for hydrocarbons. 

Chemipack ® C18 

Chemipack ® C18 is a chemically bonded GC packing. 

Because it is bonded, it has high thermal stability, zero 

bleed and stability to water. These properties make it ideal for 

GC/MS or high sensitivity GC with any detector. The functional 

group bonded to the silica is octadecyl. 

Chemipack ® 


Chemipack ® C18, 80/100, 320°C 15mL 2768 


6ft x 1/8" SS Packed with Chemipack ® C18, 

80/100, 320°C 

ea 2768PC 

Carbosphere—Carbon Molecular Sieve 

Carbosphere exhibits properties similar to molecular sieves. 

It has a surface area of about 1000m 2 /g with a pore size 

of about 13Å. Carbosphere separates oxygen, nitrogen, 

methane, carbon monoxide, and carbon dioxide in the same 

run. It also separates air, carbon dioxide, and the C1–C2 

hydrocarbons. At ambient temperatures, hydrogen will elute 

before oxygen. 

Carbosphere resolves trace amounts of methane and 

acetylene in ethylene and even trace amounts of methane, 

acetylene, and ethylene in ethane. The carbon molecular 

sieves also separate oxides of nitrogen and sulfur. Because 

Carbosphere is very nonretentive for water, rapid 

separations of organics, such as formaldehyde and methanol 

in water are possible. 


Unibeads S 


1S, 60/80, 22A 25g 2752 

1S, 80/100, 22A 25g 2758 

2S, 60/80, 60A 25g 2760 

2S, 80/100, 60A 25g 2762 

3S, 60/80, 100A 25g 2764 

3S, 80/100, 100A 25g 2766 


6ft x 1/8" SS Packed with Unibeads 1S, 60/80 ea 2752PC 

6ft x 1/8" SS Packed with Unibeads 2S, 60/80 ea 2760PC 

It is important to remove every trace of oxygen from the 

carrier gas used on Carbosphere columns. This is essential 

if you are doing trace analysis or using temperatures above 

200°C. An oxygen purifier such as Oxy-Trap or Indicating 

Oxy-Trap is ideal for removal of oxygen from carrier gas. 

Carbosphere can be affected by contaminants in the air 

and in carrier gas. Store under pure nitrogen or argon gas. 

Carbosphere 


60/80 5g 5680 

80/100 5g 5682 


6ft x 1/8" SS Packed with Carbosphere, 60/80 ea 5680PC 




Need an oxygen trap? 

See page 271. 

4653 


253



GC Packings 

Molecular Sieves 

• For drying applications 

Molecular sieves are synthetic alkali metal alumino-silicates 

with various cations. They are used for the separation of 

fixed gases and drying of liquid or gas streams. They can 

be reactivated by heating at 250°C for 12 hours; 300°C for 

4 hours; or 350°C for 2 hours. 

Molecular Sieves 

100g 

Part No. 

1kg 

Part No. 

Description 

Molecular Sieve 3A 

1/8" Pellets 05252 05253 

1/16" Pellets 05254 05255 

40/60 05306 05295 

60/80 05307 05298 

80/100 05308 05301 

100/120 05309 05304 


8/12 Beads 05256 05257 

1/8" Pellets 05258 05259 

1/16" Pellets 05260 05261 

40/60 5620 05345 

60/80 5622 05348 

80/100 5624 05351 

100/120 5625 05355 


1/8" Pellets 05264 05265 

1/16" Pellets 5633 05267 

40/60 5626 05327 

60/80 5328 05330 

80/100 5630 05333 

100/120 5632 05336 

Molecular Sieve 13X 

8/12 Beads 05268 05269 

1/8" Pellets 05270 05271 

1/16" Pellets 05272 05273 

40/60 5634 05493 

60/80 5636 05496 

80/100 5638 05499 

100/120 5640 05502 

Washed Molecular Sieves 

• For chromatography applications 

The usual gas chromatographic grades of molecular sieves 

have a fine dust adhering to each particle which cannot be 

removed simply by careful sieving. This dust is responsible for 

poorer separations and higher pressure drops than molecular 

sieves which are essentially dust-free. 

The washed molecular sieves offered here have been 

carefully cleaned of dust particles by washing with distilled 

water. The cleaned product is then activated and sealed in 

glass jars. Molecular sieves are typically reactivated at a 

temperature of 300°C for four hours. 

Washed Molecular Sieves 


Washed Molecular Sieve 5A 

40/60 50g 5602 

60/80 50g 5604 

80/100 50g 5605 

100/120 50g 5606 

Washed Molecular Sieve 13X 

40/60 50g 57728 

60/80 50g 57730 

80/100 50g 57732 

100/120 50g 57734 


6ft x 1/8" SS Packed with 80/100 Washed 5A ea 5605PC 

6ft x 1/8" SS Packed with 80/100 Washed 13X ea 5773PC 

5ft x 1/8" SS Packed with 60/80 Washed 13X ea 57730PC 







Graphitized Carbon Blacks 

Carbograph Packings 

• High speed analysis 

• Extremely hydrophobic—ideal for aqueous injections 

Equivalent to the Carbopack packings. Carbograph 

packings can be modified with a stationary phase giving them 

unique selectivities. 

Carbograph 1 

Equivalent to Carbopack B. It has a surface area of 

100m 2 /g. Carbograph 1-TD has a coarse mesh of 20/40 

which is suitable for thermal desorption work. Maximum 

temperature 500°C. 

Carbograph 1SC 

Similar to Carbopack BHT, specifically for the separation of 

sulfur compounds. It has a mesh of 40/60, and a maximum 

temperature of 225°C. 

Carbograph 2 

Equivalent to Carbopack C. It has a specific surface of 

10m 2 /g for the separation of volatile organic compounds. 

Carbograph 2-TD has a coarse mesh of 20/40 which 

is suitable for thermal desorption work. Maximum 

temperature 500°C. 

Carbograph 


Uncoated 

Carbograph 1-TD, 20/40, >500°C 15g 1740 

Carbograph 1, 60/80, >500°C 10g 1722 

Carbograph 1 80/120, >500°C 10g 1724 

Carbograph 2-TD, 20/40, >500°C 15g 1745 

Carbograph 2, 60/80, >500°C 10g 1726 

Carbograph 2, 80/100, >500°C 10g 1728 

Carbograph 1-SC, 40/60, 225°C 10g 1734 

Coated 

1% AT-1000 on Carbograph 1 60/80, 225°C 15g 1733 

0.1% AT-1000 on Carbograph 2 

15g 1737 

80/100, 225°C 

4% Carbowax ® 20M on Carbograph 1 DA 15g 17271 

80/120, 200°C 

0.2% Carbowax ® 1500 on Carbograph 2 15g 1725 

60/80, 175°C 

0.2% Carbowax ® 1500 on Carbograph 2 15g 1720 

80/100, 175°C 

5% Carbowax ® 20M on Carbograph 

15g 1729 

1 AW 80/120, 225°C 

6.6% Carbowax ® 20M on Carbograph 1 AW 15g 1731 

80/120, 225°C 

4% Carbowax ® 20M + 0.8% KOH on 

15g 1735 

Carbograph 1 60/80, 220°C 

0.19% Picric Acid on Carbograph 2 

15g 1736 

80/100, 120°C 

0.8% THEED on Carbograph 2 80/100, 125°C 15g 1942 

Graphpac-GC and -GB Packings 

• Graphitized carbon 

• Inert, non-specific surface 

• Unique separation properties 

• Optimized phase loading 

• Hydrophobic—ideal for aqueous injections 

Graphpac-GC has an average surface area of 

10–13m 2 /g, while Graphpac-GB has a larger surface area 

of 100–110m 2 /g. Both types interact with samples almost 

completely by non-specific adsorption. Which results in unique 

separations not achievable with fused silica open tubular 

(FSOT) capillary columns. Alcohols, hydrocarbons, and acids 

are separated in series according to carbon number. 

The carbons possess partitioning capabilities as uncoated 

packings, use stationary phases to modify the selectivity of 

these carbons. 

Graphpac 


Graphpac-GC 

0.1% AT-1000, 225°C, 80/100 15g 8542 

0.2% Carbowax ® 1500, 175°C, 80/100 15g 8546 

0.19% Picric Acid, 120°C, 80/100 15g 8550 

0.8% THEED, 125°C, 80/100 15g 8552 

Uncoated, 60/80 15g 8536 

Uncoated, 80/100 15g 8538 

Custom—Specify Phase and % Loading 15g C-2200 

Graphpac-GB 

1% AT-1000, 225°C, 60/80 15g 8517 

4% Carbowax ® 20M + 0.8% KOH Amines, 15g 8519 

220°C, 60/80 

Uncoated, 60/80 15g 8513 

Uncoated, Acid Washed, 60/80 15g 8533 

Custom—Specify Phase, Mesh, and % Loading 15g C-2200 

Custom, Acid Washed—Specify Phase, Mesh, 15g C-2300 

and % Loading 


6ft x 1/8" SS Packed with 0.1% AT-1000, ea 8542PC 

225°C, 80/100 

6ft x 1/8" SS Packed with 0.2% Carbowax ® 1500, ea 8546PC 

175°C, 80/100 

7ft x 1/8" SS Packed with 0.19% Picric Acid, ea 85501PC 

120°C 80/100 

10ft x 1/8" SS Packed with 5% Krytox ® 143°C, 

60/80 

ea 85222 



255



Porous Polymers 

Tenax ® -TA (GC) Polymers 

• Stable baseline after conditioning 

• Short retention times 

• Low affinity for water 

• Maximum temperature limit is 350°C 

Tenax ® -TA is a porous polymer that is based on 2,6-Diphenylp-phenylene 

Oxide. It has replaced Tenax ® -GC. Tenax ® -TA 

can be used as a packing and as a trapping material. Both the 

EPA and NIOSH specify the use of Tenax ® in their standard 

methods. Tenax ® is particularly useful for the analysis of high 

boiling compounds such as alcohols, polyethylene glycols, 

diols, phenols, monoamines and diamines, ethanolamines, 

aldehydes, ketones, and chlorinated aromatics. 

Tenax ® -TA (GC) Specifications 

Specific Surface Area: 35m 2 /g 

Pore Volume: 

2.4g/cc 

Average Pore Size: 

200nm 

Density: 

0.25g/cc 

Tenax ® -TA (GC) 


20/35 10g 04914 

20/35 100g 049141 

20/35 500g 049142 

20/35 1000g 049143 

35/60 10g 04915 

35/60 100g 049151 

35/60 500g 049152 

35/60 1000g 049153 

60/80 10g 04916 

60/80 100g 049161 

60/80 500g 049162 

60/80 1000g 049163 

80/100 10g 04917 

80/100 100g 049171 

80/100 500g 049172 

80/100 1000g 049173 

Pretreated Tenax ® -TA 

Cleaned by Solvent Extraction — C-4196 

Stocked Packed Columns 

6ft x 1/8" SS Packed with Tenax ® -TA Porous ea 4900PC 

Polymer, 60/80 

6ft x 1/8" SS Packed with Tenax ® -TA Porous ea 4901PC 


Tenax ® -TA — Membranes, 47mm 10 4943 

Tenax ® -GR Polymers 

• Superior properties as trapping material 

• Maximum temperature limit is 350°C 

Tenax ® -GR contains 23% graphitized carbon as an integral 

part of the material. This is not an admixture; the graphitized 

carbon is co-precipitated with the Tenax ® polymer. The 

resulting material gives higher breakthrough volumes for 

most materials, yet is less water retentive than Tenax ® -TA. 

When using this material for packing GC columns, better peak 

symmetry is observed. Surface area is 24.1m 2 /g. 

Tenax ® -GR 


20/35 10g 4933 

20/35 100g 49331 

20/35 500g 49332 

20/35 1000g 49333 

35/60 10g 4935 

35/60 100g 49351 

35/60 500g 49352 

35/60 1000g 49353 

60/80 10g 4937 

60/80 100g 49371 

60/80 500g 49372 

60/80 1000g 49373 

80/100 10g 4939 

80/100 100g 49391 

80/100 500g 49392 

80/100 1000g 49393 

Stocked Packed Columns 

6ft x 1/8" SS Packed with Tenax ® -GR Porous 


ea 4937PC 

tech tip 

Conditioning 

We highly recommend that Tenax ® , like all other porous polymers, 

be preconditioned before being packed into GC columns. This 

removes most of the shrinkage of the polymer and minimizes the 

need for further conditioning. Tenax ® can be expected to shrink 

further in use. 



GC Packings 

HayeSep ® Porous Polymer 

• Pre-purified porous polymers for GC 

• Less bleed and shrinkage than other porous polymers 

• Cleaned and conditioned to 200°C (165°C for N & T) 

HayeSep ® polymers were developed to solve room 

temperature separation of N2, O2, Ar and CO; ppm H2O in 

the presence of HCl, Cl2 etc. The HayeSep ® D series better 

separates the fixed gases to the C2 hydrocarbons. 

HayeSep ® Specifications 

Series Polymer Composition 

Surface 

Area Polarity 

Q DVB* 582m 2 /g Non-Polar 

P DVB*/Styrene 165m 2 /g Slightly Polar 

R DVB*/N-Vinyl-2-pyrrolidinone 344m 2 /g Moderately Polar 

S DVB*/4-Vinyl-pyridine 583m 2 /g Moderately Polar 

T EGDM** 250m 2 /g Very Polar 

N DVB*/EGDM** 405m 2 /g Very Polar 

A DVB*/EGDM** (high purity) 526m 2 /g Moderately Polar 

B DVB*/Polyethyleneimine 608m 2 /g Very Polar 

C DVB*/Acrylonitrile 442m 2 /g Moderately Polar 

D DVB* (high purity) 795m 2 /g Non-Polar 

*Divinylbenzene. **Ethyleneglycoldimethacrylate. 

Comparison of HayeSep ® “D” Formulations † 

Table 1—Retention Time (in minutes) 

Air CH4 CO2 C2H2 C2H4 C2H6 H2O 

DIP 0.9 1.7 3.1 5.4 5.8 8.3 9.0 

D 0.9 1.7 3.1 5.8 6.1 8.4 8.6 

DOX 1.0 1.8 3.2 5.9 6.3 8.5 8.3 

DB 0.9 1.6 3.1 6.1 6.6 8.7 8.1 

† 

Retention data based on 10ft x 1/8" stainless steel at 45°C, 30mL/min. 

HayeSep ® 

Description 

Mesh 

Temp. Limit 

max. Qty. Part No. 

HayeSep ® A 60/80 165°C 75cc 2818 

80/100 165°C 75cc 2819 

100/120 165°C 75cc 2820 

20/140 165°C 75cc 2821 

HayeSep ® B 60/80 190°C 75cc 2822 

80/100 190°C 75cc 2823 

100/120 190°C 75cc 2824 

HayeSep ® C 60/80 250°C 75cc 2825 

80/100 250°C 75cc 2826 

100/120 250°C 75cc 2827 

HayeSep ® D 60/80 290°C 75cc 2828 

80/100 290°C 75cc 2829 

100/120 290°C 75cc 2830 

HayeSep ® (continued) 

Description 

Mesh 

Temp. Limit 


HayeSep ® DB 60/80 290°C 75cc 2834 

80/100 290°C 75cc 2835 

100/120 290°C 75cc 2836 

HayeSep ® DIP 60/80 290°C 75cc 2831 

80/100 290°C 75cc 2832 

100/120 290°C 75cc 2833 

HayeSep ® N 60/80 165°C 75cc 2815 

80/100 165°C 75cc 2816 

100/120 165°C 75cc 2817 

HayeSep ® P 60/80 250°C 75cc 2803 

80/100 250°C 75cc 2804 

100/120 250°C 75cc 2805 

HayeSep ® Q 60/80 275°C 75cc 2800 

80/100 275°C 75cc 2801 

100/120 275°C 75cc 2802 

HayeSep ® R 60/80 250°C 75cc 2806 

80/100 250°C 75cc 2807 

100/120 250°C 75cc 2808 

HayeSep ® S 60/80 250°C 75cc 2809 

80/100 250°C 75cc 2810 

100/120 250°C 75cc 2811 

HayeSep ® T 60/80 165°C 75cc 2812 

80/100 165°C 75cc 2813 

100/120 165°C 75cc 2814 


40ft x 1/8" SS Packed 120/140 165°C ea 28211 

with HayeSep ® A 

10ft x 1/8" SS Packed 100/120 290°C ea 28301PC 

with HayeSep ® D 

20ft x 1/8" NI Packed 100/120 290°C ea 27082PC 





with HayeSep ® DB 

9ft x 1/8" SS 

100/120 290°C ea 2833PC 

HayeSep ® DIP 


with HayeSep ® P 

6ft x 1/8" SS Packed 80/100 250°C 75cc 2804PC 

with HayeSep ® P 


with HayeSep ® Q 


with HayeSep ® Q 


with HayeSep ® R 


with HayeSep ® S 


with HayeSep ® T 

5ft x 1/8" SS Packed 

with HayeSep ® T 

100/120 165°C ea 2814PC 



257


GC Packings 

Century Series Chromosorb ® 

Century Series Chromosorb ® porous polymers have a rigid 

structure and distinct pore size. They are packed into columns 

in the normal manner and do not require a liquid coating. 

We recommend preconditioning all porous polymers before 

packing columns. 

Porapak ® 

Porapak ® GC packings are cross-linked polymers which 

can be used directly in GC columns without a stationary 

phase coating. Acetone washing of Porapaks ® improves 

performance. 

Chromosorb ® Specifications 

Series Polymer Composition Surface Area Polarity 

101 DVB*/Styrene >50m 2 /g Non-Polar 

102 DVB*/Styrene 300–400m 2 /g Slightly Polar 

103 Cross-linked Polystyrene** 15–25m 2 /g Non-Polar 

105 Polyaromatic 600–700m 2 /g Mod. Polar 

106 Cross-linked Polystyrene 700–800m 2 /g Non-Polar 

107 Cross-linked Acrylic Ester 400–500m 2 /g Polar 

108 Cross-linked Acrylic 100–200m 2 /g Polar 

*Divinylbenzene. **The surface is basic. 

Porapak ® Specifications 

Series Polymer Composition Surface Area Polarity 

Q DVB*/Ethylvinylbenzene 500–600m 2 /g Slightly Polar 

P DVB*/Styrene 100–200m 2 /g Non-Polar 

R DVB*/Vinyl pyrrolidinone 450–600m 2 /g Mod. Polar 

S DVB*/Vinyl pyridine 300–450m 2 /g Mod. Polar 

T EGDM** 225–350m 2 /g Polar 

N DVB*/Vinyl pyrrolidinone 250–350m 2 /g Very Polar 

*Divinylbenzene. **Ethyleneglycoldimethacrylate. 


Chromosorb ® 

Description 

Mesh 

Temp. Limit* 


Chromosorb ® 101 60/80 275/325°C 50g 2405 

80/100 275/325°C 50g 2406 

100/120 275/325°C 50g 2407 

Chromosorb ® 102 60/80 250/300°C 50g 2408 

80/100 250/300°C 50g 2409 

100/120 250/300°C 50g 2410 

Chromosorb ® 103 60/80 250/300°C 50g 2411 

80/100 250/300°C 50g 2412 

100/120 250/300°C 50g 2413 

Chromosorb ® 105 60/80 250/275°C 50g 2417 

80/100 250/275°C 50g 2418 

100/120 250/275°C 50g 2419 

Chromosorb ® 106, 60/80 250/275°C 50g 2420 

80/100 250/275°C 50g 2421 

100/120 250/275°C 50g 2422 

Chromosorb ® 107 60/80 250/275°C 50g 2423 

80/100 250/275°C 50g 2424 

100/120 250/275°C 50g 2425 

Chromosorb ® 108 60/80 250/275°C 50g 2426 

80/100 250/275°C 50g 2427 

100/120 250/275°C 50g 2428 

Preconditioning Service † per bottle C-14030 

† 

Specify bulk packing part no. and preconditioning service part no. 


Porapak ® 

Description 

Mesh 

Temp. Limit 


Porapak ® Q 50/80 250°C 26g 2700 

80/100 250°C 26g 2701 

100/120 250°C 26g 2702 

Porapak ® P 50/80 250°C 20g 2703 

80/100 250°C 20g 2704 

100/120 250°C 20g 2705 

Porapak ® R 50/80 250°C 24g 2706 

80/100 250°C 24g 2707 

100/120 250°C 24g 2708 

Porapak ® S 50/80 250°C 26g 2709 

80/100 250°C 26g 2710 

100/120 250°C 26g 2711 

Porapak ® T 50/80 190°C 31g 2712 

80/100 190°C 31g 2713 

100/120 190°C 31g 2714 

Porapak ® N 50/80 190°C 29g 2715 

80/100 190°C 29g 2716 

100/120 190°C 29g 2717 

Porapak ® PS 50/80 250°C 20g 2721 

80/100 250°C 20g 2722 

100/120 250°C 20g 2723 

Acetone Washing Service † per bottle C-2700 

† 

Specify bulk packing part no. in addition to acetone washing part no. 

tech tip 





condition. 



Capillary Connectors 

Universal Capillary Connectors 

• One size fits tubing from 0.25mm i.d. to 0.53mm i.d. 

• Available in straight or Y configurations 

Capillary connectors form a gas-tight seal when heated to 

typical GC temperatures. The angled connectors conform 

to the curvature of a fused silica capillary column. To create 

a permanent seal, add a small amount of polyimide resin to 

the outside of the capillary tube before inserting. Order under 

“Accessories” below. 

Universal Straight Connector 

0017 

Featherweight Capillary Union 

• Lightweight 

• High temperature Vespel ® 

construction 

• Accommodates all tubing sizes 

Featherweight Capillary Union 

Description 

Part No. 

Featherweight Union with 2 Nuts* 28990 

Vespel ® Ferrules for Use with Featherweight Union 

Vespel ® Ferrule, 0.8mm i.d. 28992 



*Order ferrules separately. 

5717 

Connectors 


Straight Connectors 

Fused Silica 

Universal Straight Connector 5 20400 


Angled Universal Connector 5 20694 

Angled Universal Connector 25 20695 

Borosilicate 



Y-Connectors 

Fused Silica 

Universal Y-Connector 1 20405 

Angled Universal Y-Connector 1 20500 

Borosilicate 



Accessories 

Polyimide Sealing Resin 5g 5825 

Fused Silica Outlet Splitters 

• Accommodates capillaries 

from 0.22 to 0.32mm i.d. 

and provides a 1:1 ratio 

• Includes a glass-lined SS 

bulkhead union, a 0.4mm i.d. 

two-hole Vespel ® graphite 

ferrule and two lengths of 

deactivated fused silica tubing 

Use this inert outlet splitter with two detectors or one detector 

and a sample collection device. 

Outlet Splitter 

Description 

Part No. 

Outlet Splitter 14898 

6628 

gc accessories | capillary 

Valco ® Zero-Dead Volume Union 

Use these stainless steel unions to make connections with fused silica capillaries. 

Unions feature specially designed Vespel ® /graphite ferrules and are bored through. 

Table 1 shows the parts needed for different connections. 

6021 

Table 1 

Connect From: to 0.25mm i.d. Fused Silica to 0.32mm i.d. Fused Silica to 0.53mm i.d. Fused Silica to 1/16" SS Tubing 

0.25mm i.d. Fused Silica 9068 9068 + 907110 45849 + 907010 + 903410 45849 + 907010 

0.32mm i.d. Fused Silica 9069 + 907010 9069 45849 + 907110 + 903410 45849 + 907110 

0.53mm i.d. Fused Silica 45849 + 907010 + 903410 45849 + 907110 + 903410 9031 45835 + 903410 

1/16" SS Tubing 45849 + 907010 45849 + 907110 45835 + 903410 45835 

Valco ® Zero-Dead Volume Unions 

Description and Valco ® No. 

Part No. 

Zero Dead-Volume Unions 

1/32", 0.4mm (Valco ® ZU.5TFS.4) 9068 

1/32", 0.5mm (Valco ® ZU.5TFS.5) 9069 

1/16", 0.8mm (Valco ® ZU1TFS.8) 9031 

1/16"–1/32", 1/32" Bore (Valco ® ZRU1.5T) 45849* 

1/16", 1/16" Bore (Valco ® ZU1T) 45835* 

Valco ® Reducing Ferrules 

Description and Valco ® No. 

Part No. 

Valco ® Reducing Ferrules* 

1/32" to 0.4mm (Valco ® FS.4), 10/pk 907010 

1/32" to 0.5mm (Valco ® FS.5), 10/pk 907110 

1/16" to 0.8mm (Valco ® FS1.8), 10/pk 903410 

*Includes 2 stainless steel ferrules. 


259



Capillary Guards and Tubing 

Universal Guard Column Kits 

• Universal kits connect 

to any capillary 

column 

• Guard columns are 

deactivated 

Universal guard column kits contain three 5m deactivated 

capillaries, three-universal fused silica connectors, ceramic 

cutter, and instructions. Order sealing resin separately under 

“Accessories” to reinforce connections. Replacement kit parts 

are available below. 

Universal Guard Column kits 

Description 

Part No. 

Universal Guard Column Kits 

0.53mm Universal Guard Column Kit 13992 




Universal Connectors, 5/pk 20400 

Angled Universal Connector, 5/pk 20694 

5m x 0.53mm i.d. Capillaries, 5/pk 13990 



Accessories 

Polyimide Sealing Resin, 5g 5825 

20X Magnifier 3163 

Deactivated Guard Columns 

• Three different deactivation 

polarities 


These guard columns protect 

your analytical column by 

collecting non-volatile, pyrolyzable, 

or particulate contamination. 

Deactivated Guard Columns 

Description 

Part No. 

Non-Polar 

0.25mm x 5m 19063 

0.32mm x 5m 19064 

0.53mm x 5m 19065 

Intermediate Polar 

0.25mm x 5m 19054 

0.32mm x 5m 19055 

0.53mm x 5m 19056 

Polar 

0.25mm x 5m 19057 

0.32mm x 5m 19058 

0.53mm x 5m 19059 

6630 

6624 

Fused Silica Capillary Tubing 

• Deactivated and 

undeactivated tubing 

available 

• Available mounted on 

a cage 

Alltech provides the finest 

quality fused silica capillary 

tubing either untreated or 

deactivated by our proprietary 

high temperature process. Use 

tubing to prepare your own 

capillaries or guard columns. 


i.d. Qty. Length Part No. 

Deactivated Tubing with Cage 

0.18mm — 10m 602026 

0.18mm — 20m 602028 

0.18mm — 40m 602029 

0.25mm — 15m 602915 

0.25mm — 30m 602930 

0.25mm — 60m 602960 

0.32mm — 15m 603915 

0.32mm — 30m 603930 

0.32mm — 60m 603960 

0.45mm — 15m 602030 

0.45mm — 30m 602032 

0.45mm — 60m 602034 

0.53mm — 15m 605915 

0.53mm — 30m 605930 

0.53mm — 60m 605960 

Deactivated Tubing, Without Cage 

0.05mm — per meter 602038 

0.10mm — per meter 602037 

0.18mm — per meter 602010 

0.25mm — per meter 602640 

0.25mm x 1m 5/pk — 5827 

0.25mm x 2m 5/pk — 5829 

0.32mm — per meter 603640 

0.32mm x 1m 5/pk — 5831 

0.32mm x 2m 5/pk — 5833 

0.45mm — per meter 602012 

0.53mm — per meter 605640 

0.53mm x 1m 5/pk — 5832 

0.53mm x 2m 5/pk — 5834 

Undeactivated Tubing, Without Cage 

0.05mm — per meter 602035 

0.10mm — per meter 602036 

0.18mm — per meter 19603 

0.25mm — per meter 602600 

0.32mm — per meter 603600 

0.45mm — per meter 19602 

0.53mm — per meter 605600 

6631 



Capillary Accessories 

Capillary Cages 

These cages are made of a heavy 

gauge stainless steel and are 

electropolished to prevent abrasion. 

All are 7 inches in diameter. 

5715 

Capillary Endcaps 

Increase column life by preventing contaminants 

from entering column during storage. 

Capillary Cages 

Description 

Part No. 

7" x 3/4" Cage 75200 

7" x 1-1/4" Cage 75201 

7" x 1-3/4" Cage 75202 

High Temperature String, 10ft 6344 

Capillary Column Washer 

This easy to use column washer is designed for 

solvent flushing of bonded or cross-linked fused silica 

capillaries. It is made of heavy-duty glass and will 

hold up to 20mL of solvent. Maximum pressure is 

125psig. Order appropriate sized ferrules separately 

below. 

Capillary Column Washer 


Capillary Column Washer ea 5819 

Ferrules 

1/8" to 0.4mm VG1 10 200/0.4-VG1 

1/8" to 0.5mm VG1 10 200/0.5-VG1 

1/8" to 0.8mm VG1 10 200/0.8-VG1 

tech tip 

How much solvent is needed to wash your column? 

Refer to the table below. 

Approximate Column Volume Required 

Length 0.25mm i.d. 0.32mm i.d. 0.53mm i.d. 

10m 0.5mL 0.8mL 2.2mL 

15m 0.7mL 1.2mL 3.3mL 

25m 1.2mL 2.0mL 5.5mL 

30m 1.5mL 2.4mL 6.6mL 

50m 2.5mL 4.0mL 11.0mL 

60m 2.9mL 4.8mL 13.2mL 

5719 

Capillary Endcaps 


Fused Silica 2 20699 

Fused Silica 20 20701 

Silicone 10 4018 

Split-Vent Trap Kit 

• Charcoal filter cartridges are 

economical and easy to replace 

• Adsorbs toxic vapors 

• Connects directly to Agilent GCs 

Alltech’s split-vent trap kit effectively adsorbs toxic or 

noxious vapors that might otherwise be released into 

the workplace. The trap has a 1/8" fitting to connect 

directly to Agilent GCs and can be adapted for use 

with most other GCs. 

The split-vent trap features a corrosion-resistant 

base and a disposable NIOSH-type charcoal filter 

cartridge that is easy to replace. Order replacement 

cartridges below. 

What’s included: split-vent trap, three charcoal 

cartridges, cartridge scorer, and tip breaker. 

Split-Vent Trap Kit 

Description 

Part No. 

Split-Vent Trap Kit 5870 

Replacement Charcoal Cartridge, 10/pk 5872 

1/8" Stainless Steel Union 14165 

6632 

5702 



261


gc accessories | packed 


Attachable Funnel 

This anodized aluminum funnel attaches 

easily to 1/8" and 1/4" o.d. glass and metal 

tubing—ideal for column packing. Funnel 

can be disassembled for cleaning. Order 

adapters separately to attach funnel to 

columns with fittings already connected. 

Replacement o-rings are also available below. 

Attachable Funnel 

Description 

Part No. 

Attachable Funnel 4008 

Adapters 

for 1/8" Column 4050 




Replacement O-rings, 12/pk 4010 

GC Column Vibrator 

This vibrator is ideal for use when filling GC 

columns, including glass columns. This two-speed 

unit comes supplied with a 7-foot cord and U.S. 

standard plug. 

GC Column Vibrator 

Description 

Part No. 

110V 4012 


• Use these plugs to seal the ends 

of columns 

Simply slip plug into your ferrule and nut, 

and attach to your fittings. Ferrules will not 

lock in place. Plugs are reusable. 


Size Qty. Part No. 

1/16" 25 2898 

1/8" 25 2900 

1/4" 25 2902 

5079 

6660 

5087 

Premium Glass Wool 

Untreated—A soft fine denier Pyrex ® wool, 

primarily used for non-chromatographic 

applications. 

Silane Treated—Fine wool treated with 

di-methyldichlorosilane (DMDCS) to yield an inert 

material that won't decompose sensitive compounds. 

This material is ideal for most chromatographic applications. 

Phosphoric Acid (H3PO4), Treated—For analysis of acidic 

compounds such as free acids, phenols, and glycols. 

Quartz Wool—Made from high-purity quartz, extremely low 

in metallic oxide impurities. 

PTFE Wool—Commonly used as a plug for packed columns 

when analyzing for low levels of sulfur. 

Pesticide Grade Glass Wool—A superbly inert glass wool 

ideal for use with electron capture, nitrogen-phosphorous, 

flame photometric, alkali-bead flame, and all electrolytic 

detectors. 

Glass Wool Tools 

Glass Wool Inserter/Remover 

Wool Inserter 

Premium Glass Wool 


Glass Wool 

Untreated Glass Wool 50g 4035 

Silane-Treated Glass Wool 50g 4037 

Silane-Treated Glass Wool 250g 4038 

Phosphoric Acid-Treated Glass Wool 25g 4039 

Quartz Wool 10g 4033 

Silane-Treated Quartz Wool 10g 4233 

PTFE Wool 10g 4736 

Pesticide-Grade Glass Wool 10g 4034 

Glass Wool Tools 

Glass Wool Inserter/Remover 2 6292 

Wool Inserter ea 6285 

5075 

5088 

5089 




GC End Caps 

These end caps are non-shredding, 

flexible, and easily applied and removed. 

They provide a positive barrier to moisture 

and air. The 5/16" size fits over 1/4" 

columns that have ferrules swaged on the ends. 

The 3/16" size fits 1/8" columns with ferrules in place. 

GC End Caps 

Tube o.d. Qty. Part No. 

1/16" 100 4020 

1/8" 100 4021 

3/16" 100 4022 

1/4" 100 4023 

5/16" 100 4025 

3/8" 100 4024 

Glass Column Repair Kits 

This repair kit includes a 1ft (30.5cm) 

piece of shrinkable TFE-FEP tubing that 

encapsulates the broken section creating 

a tight and reliable seal. 

Easy to apply, just slip over one end of the 

broken tubes, butt the tubes, and heat to 

330°C with a heat gun. 


Tubing Size 

Part No. 


Up to 0.036" (0.91mm) 5800 

Up to 0.060" (1.52mm) 5801 

Up to 0.130" (3.30mm) 5802 

0.125" (3.18mm) to 0.250" (6.35mm) 5803 

Heat Gun 

110V 2518 

220V 2518-220V 

Column Tags 

Use these aluminum tags to create 

a permanent heat resistant record of 

pertinent column information. Writing 

surface of tag is 1" x 2" (25.4 x 50.8mm). 

Column Tags 


Embossed GC Tags 100 8011 

Embossed GC Tags 25 8012 

Embossed LC Tags 25 8005 

Plain Tags 100 8009 

5085 

5082 

5074 

Pressed Frits 

These frits are precision-fabricated 

from Type 316 stainless steel and 

are ideal for sealing the ends of 

GC columns. 

Pressed frits are easy to install. 

Drill the column ends to the proper 

diameter and depth using the drill 

bits sold below. Rest the frit on 

top of the tubing and gently tap 

it into place. 

Drill Bits 

These drill bits are specifically 

designed to prepare tubing for frit 

installation. They include a bushing that 

acts as a stop to control the depth of the hole. 

Vibrograver 

Use for engraving or column 

packing. A carbide point is 

included. Remove the point to 

use for column packing. Order 

replacements below. Vibrograver 

is not CE marked. 

Pressed 

Frits 

Column 

Stainless Steel Pressed Frits 

Pore Diameter Qty. For Use With 1/8" o.d. Tubing For Use with 1/4" o.d. Tubing 

0.5µm 25 702625 — 

2.0µm 25 703425 703525 

5.0µm 25 702825 702925 

10.0µm 25 703025 703125 

20.0µm 25 703225 703325 

Drill Bits 

Diameter Qty. Part No. 

for 1/8" Frits ea 7032 

for 1/4" Frits ea 7033 

Vibrograver 

Description 

Part No. 

Vibrograver 

110V 8006 

220V 8018 

Replacement Point 

Diamond 15044 

Carbide 15045 

5086 

1050 

6633 

5076 

gc accessories | packed 


263


Cylinder Accessories 

Cylinder Status Tags 

Flow Controllers 

Flow Diverter 

Cylinder status tags help to organize and 

positively identify cylinders. 

Cylinder Status Tags 

Description 

Part No. 

Cylinder Status Tags, 25/pk 8004 

Cylinder Tools 

5250 

Easily divert the gas stream from 

your detector to your soapfilm 

flowmeter. One Flow Diverter 

is required for each gas. It is 

supplied with 1/8" fittings. 

Flow Diverter 

Description 

Part No. 

Flow Diverter 4200 

5226 

Conveniently open valves, tighten gauges, connections, 

regulators, etc. 

Adjustable Flow Controller 

• Control flow rates from 0.5mL/min up to 

1.6L/min 

gc accessories | gas flow 

Cylinder Holders 

Prevent toppling of 

cylinders with these 

cast aluminum cylinder 

holders. Holders feature 

a nylon web strap with a 

sturdy buckle. Cylinder 

holders handle cylinders 

from 4 to 15 inches in 

diameter. Choose from 

benchtop or wall mount 

styles. 

6777 

Cylinder Valve & Cap Wrench 

Matheson Switchover Systems 

• Automatically change cylinders 

• Compact design minimizes wall 

space consumption 

9-way Cylinder Wrench 

Cylinder Tools 

Description 

Part No. 

Cylinder Valve & Cap Wrench 37135 

9-Way Cylinder Wrench 412933 

Wall mount cylinder holder 

with safety strap 

Cylinder Holders 

Description 

Part No. 

Bench Top Model with Cylinder Strap 37132 

Wall Mount Model with Cylinder Strap 37133 

5178 

6098 

This adjustable flow controller features a user 

variable span adjustment permitting it to be 

used for a variety of flow ranges. Use the 

ten-turn knob to easily adjust flow rates from 

zero to maximum. Pipe adapters for 1/8" o.d. 

tubing are included. 

Flow Controller Specifications 

Flow Rate: 

Adjustable from 1.6mL/min to 0.5L/min 

Body: 

Aluminum 

Diaphragm: Stainless Steel 

Max Pressure: 250psig 

Max Temp: 120°C 

Ports: 

1/8" MPT 

Flow Controller 

Description 

Part No. 

Adjustable Flow Controller 8149 

Eco-Mizer 

• Conserve expensive gases during 

standby periods 

• Decrease laboratory operating expenses 

• Directly connects to 1/8" o.d. tubing 

The Eco-Mizer is a compact standby valve 

designed to conserve gases overnight and 

during other non-use periods. The adjustable 

fine metering valve sets the standby flow rate and a 

concentric, independent valve allows selection of either 

standby flow rate or straight-through flow. 

5227 

5251 

Matheson Switchover Systems 

Description 

Part No. 

Model 5234 Brass 37139 

Model 5244 SS 37143 

Model 5230 Brass with Regulator 37138 

Model 5240 SS with Regulator 37141 

6094 

Eco-Mizer 

Description 

Part No. 

Eco-Mizer 7070 



Flowmeters 

Aalborg Single-Tube Variable Area 

Flowmeters 

• Built-in needle valve controls flow 

• Magnifier lens enhances reading resolution 

• Ideal for panel mounting 

Equipped with easy to change flowtubes and a 

control valve. Order a bench stand and fittings 

separately under “Accessories” below. 

Single-Tube Flowmeter Specifications 

Accuracy: 

±5% full scale 

Repeatability: ±0.25% 


Max Temperature: 121°C 

Connections: 1/8" FPT inlet and outlet 

5198 

Aalborg Three-Tube Variable Area Flowmeter 

• Measure hydrogen, air, and carrier gases 

with one meter 

• Direct reading for hydrogen and air 

• Carrier gas tube can be correlated for 

any gas 

• Can be panel-mounted or free standing 

• Ideal for use with flame ionization detectors 

Three-Tube Flowmeter Specifications 

Accuracy: 

±5% full scale 

Repeatability: ±0.25% 


Max Temperature: 121°C 

Connections: 1/8" FPT inlet and outlet 

5197 

Single-Tube Flowmeters 

Flowtube Range Div. Part No. 

65mm Flowtubes, Direct Reading Scale 

Helium 0–120cc/min 5mL 7900 

Hydrogen 0–100cc/min 5mL 7902 

Nitrogen 0–120cc/min 5mL 7904 

Air 0–1.15L/min 0.05L 7906 

General Purpose 0–2L/min (air) 1mm 7908 

Accessories 

Bench Mount for Above Flowmeters 7920 

1/8" to 1/8" MPT Brass Connector 60038 

1/4" to 1/8" MPT Brass Connector 60042 

Need Help Timing Your Flowmeter? 

Lightweight Stopwatch 

This stopwatch counts up to 24 hours with 

0.01sec readout and has split interval function, 

alarm clock with a choice of 12- or 24-hour 

format, a calendar displaying date and day 

of the week, hourly chime option, and button 

cell battery. 

Large Display Stopwatch 

This stopwatch features a large, 1/2" LCD 

display. It counts with a resolution of 0.01sec 

and has split interval, clock and calendar 

functions. It includes a rugged ABS plastic clip, 

neck cord, leatherette case, stand clip, and a 

1.5V battery. 

Three-Tube Flowmeter 

Description 

Part No. 

Three-Tube Flowmeter 4040 

Soapfilm Flowmeter 

Economical Flow Measurement 

This soapfilm flowmeter is easy to use. Simply time the 

movement of a soap bubble between two calibration 

marks. Alltech’s soapfilm flowmeters have a unique 

bubble burster at the top to eliminate the overflow of 

the soap solution. A rubber bulb is included with each 

soapfilm flowmeter. The 100µL and 500µL flowmeters 

are complete with stand and tubing. Order soapfilm 

solution and tubing under “Accessories” below. 

Soapfilm Flowmeters 

Description 

Part No. 

Flowmeters 

100µL 14184 

500µL 14185 

1cc 4041 

10cc 4042 

25cc 4043 

100cc 4045 

1000cc 4047 

Accessories 

Soapfilm Solution, 8oz 2040 

5/16" o.d. Tygon ® Tubing, 10ft 4333 

5195 


Triple Timer 

This timer features a large easy-to-read 

display. It can time three activities at once and 

has three distinctive alarms. It has a compact 

size of 3" x 3". 

Stopwatches 

Description 

Part No. 

Lightweight Stopwatch 4069 

Large Display Stopwatch 4071 

West Bend Triple Timer 40032 


Need a leak detector? 

Ensure leak-free installation of your gas 

purifiers. See page 267 for gas leak 

detectors. 


265


Alltech ® Regulators 

A) Dual-Stage Economy Regulators 

These regulators are suitable for pressure 

control of general purpose laboratory gases 

with applications requiring constant pressure 

control and delivery regardless of supply 

pressure variations. Typical applications 

include purging, pressure testing, 

blanketing, and chamber pressurization. 

Dual-Stage Economy 

Description CGA No. Part No. 

H2, Ar/Methane 350 8101 

O2 540 8102 

He, N2, Ar, Ne 580 8103 

Air 590 8104 

Air, Breathing Quality 346 8105 

C) Single-Stage Regulators 

These regulators are suitable for secondary 

pressure control of non-corrosive, high-purity 

gases or as point of use pressure control in 

high-purity gas distribution systems. Typical 

applications include point of use laboratory 

systems, gas supply purging, and bulk gas 

distribution systems. 

5244 5246 

Single Stage 


0–40psig (delivery pressure) — 81890 




B) Dual-Stage 

High-Purity Regulators 

These regulators are suitable for secondary 

pressure control of non-corrosive or high 

purity gases for applications requiring 

constant pressure control and delivery 

regardless of supply pressure variations. Typical applications 

include gas supply purging, gas system charging, fuel gas 

supply control, and calibrations gas control. 

Dual-Stage High-Purity 


H2, Ar/Methane 350 8109 

He, N2, Ar, Ne 580 8111 


Need cylinder accesories? 

See page 264 for more information. 

best 

seller 

5245 

D) Panel Mount Regulators 

These regulators can also be used in-line. 

Order gauges and mounting nut separately. 

Panel Mount 


Panel Mount Regulator — 81896 

Panel Mounting Nut — 81895 

Pressure Gauges for Panel Mount Regulators 

0–30psig — 86800 

0–60psig — 86802 

0–100psig — 86804 

0–200psig — 86808 

5248 

Alltech ® Regulators Specifications 

A) Dual-Stage Economy B) Dual-Stage High-Purity C) Single Stage D) Panel Mount 

Body: Forged Brass Chrome-plated forged brass Chrome-plated forged brass Brass 

Bonnet: Brass-plated die cast zinc Chrome-plated die cast zinc Chrome-plated die cast zinc Acetal Resin 

Diaphragm: PTFE-lined neoprene 316L Stainless Steel 316L Stainless Steel Nitrile 

Seat and Internal Seal: PTFE PTFE PTFE Acetal Resin 

Max. Inlet Pressure: 3000psig (210 bar) 3000psig (210 bar) 3000psig (210 bar) 400psig 

Delivery Pres. Range: 0–120psig 0–120psig 0–40, 0–120, or 0–200psig — 

Delivery Pres. Gauge: 0–20psig 0–200psig 0–60, 0–200, or 0–400psig Order Separately 

Cylinder Pres. Gauge: 0–4000 psig 0–4000psig 0–4000psig 0–4000psig 

Temperature Range: -40 to 60°C -40 to 60°C -40 to 60°C -34 to 65°C 

Gauges: 2-1/2" diameter brass 2-1/2" diameter brass 2-1/2" diameter brass Order Separately 

Ports: 1/4" FPT 1/4" FPT 1/4" FPT 1/8" FPT 



Gas Leak Detection 

Alltech ® Helium Leak Detector 

Designed Specifically to Detect Helium 

Gas Leaks 

• Portable—operates up to 

6.5 hours fully charged 

• Visual and audible detection 

indicators 

• Change polarity setting to detect 

other gases 

best 

seller 

The Alltech ® Helium Leak Detector is perfect for detecting 

gas leaks in GC, GC/MS, and purge/trap systems. Leaks are 

detected via a thermal conductivity cell and indicated visually 

on the LCD display and by an audible alarm. The long, flexible 

probe makes it convenient to detect small and hard to reach 

gas leaks. Use the detector for gases other than helium 

by simply changing the polarity setting. Detector features 

a simple one-step zero adjustment and is fully portable 

operating on rechargeable Ni-Cd batteries. 

What’s included: probe, rechargeable batteries and charger 

Helium Leak Detector Specifications 

Detector: 

Thermal conductivity cell with thermistors 

Sensitivity: 0.01mL/min for Helium 

Ambient Temp: 10 to 35°C 

Display: 

LCD indicator lights 

Zero Function: Yes 

Audible Signal: Yes 

Battery Life: 6.5 hours continuous fully charged 

Dimensions: 1.6" H x 2.9" W x 6.3" L 

(4.064cm H x 7.366cm W x 16.002cm L) 

Weight: 

1lb (0.454kg) 

Note: The Alltech ® helium leak detector is not explosion-proof and should 

not be used to detect combustible or corrosive gas leaks. 

6711 

Leak-Tec ® High Temp Testing Solution 

Temperature Range is 210 to 415°F 

This testing solution wets and spreads evenly and 

quickly responding to small leaks. Leak-Tec ® is 

non-corrosive and residue free. Convenient aerosol 

can allows spray to be directed in tight spaces. 

Leak-Tec ® 


Leak-Tec ® , 10oz ea 2027 

6 2028 

Leak-Check Testing Solution 

Leak-Check is non-toxic, non-flammable, chloridefree 

and dries cleanly leaving no residue behind. 

Temperature range is 30 to 200°F. Bottle includes 

a 15" snorkel tube allowing for easy access to 

hard-to-reach spaces. 

Leak-Check 


Leak-Check, 8oz ea 2025 

12 2026 

5241 

5242 


Helium Leak Detector 

Description 

Part No. 

120 VAC charger with rechargeable Ni-Cd batteries 60229 

220 VAC charger with rechargeable Ni-Cd batteries 60231 






267


Gas Purifiers and Traps 

Why Use Gas Purifiers? 

A clean gas stream helps guarantee the quality of your GC 

analysis and the reliability of your results. It also helps prolong 

column life and minimize detector noise. Gas purifiers act 

as an insurance policy, protecting your instrumentation and 

analytical columns from these contaminants. They will not 

however, convert low purity gas into ultra high purity gas. 

Grace carries a variety of gas purifiers in two different styles. 

See Table 1 for details. 

Choosing the Right Gas Purifier for Your 

Application 

There are a variety of gas purifiers available. Some remove 

a specific contaminant while others remove multiple 

contaminants from your gas stream. There are several 

factors you need to consider when selecting purifiers for your 

application. These include contaminants potentially present in 

your gas stream, gas purity levels required, flow and pressure 

limitations, space availability, and desired convenience when 

replacing spent traps. 

Moisture, hydrocarbon, and oxygen traps are the most 

commonly used purifiers for GC applications. Moisture traps 

are typically packed with molecular sieves. After extensive 

heating, these crystalline structures lose their water of 

hydration opening a cavity that is readily filled with any 

compound that can fit. Water fits perfectly. However, it will 

also remove HCl, CO2, SO2, Cl2, or any gas with an effective 

diameter smaller than water. 

gc accessories | purifiers 

Cartridge Style 

5334 5335 

How Contaminants Affect Your Gas Stream 

In-Line Style 

Table 1—Gas Purifier Styles 

Cartridge Style 

In-Line Style 

Placement: Stands on Benchtop Freely supported on 

gas lines 

Repeat Use: Replace Cartridge Refill* 

Benefit: 

Convenient access, stops 

gas flow when changing 

cartridges 

Occupies wall space 

not bench space 

*Refills for most materials are available. See specific gas purifiers for refill kit 

part numbers. 

Follow the path that your carrier gas or detector fuel gases 

take to get to the GC. They start at either a gas tank or a gas 

generator, and travel through long lengths of tubing, pressure 

gauges, various fittings, and valves. Each is a potential culprit 

for introducing contaminants into your gas stream, which 

ultimately degrades chromatography results and shortens 

column life. 

Hydrocarbon traps are packed with an activated charcoal that 

adsorbs any organic compound larger than methane. Size 

and molecular weight of the organic contaminants will greatly 

effect the capacity of the trap, and to a smaller degree, trap 

efficiency. Activated charcoal has a higher capacity for larger 

(>C4) hydrocarbons than for smaller hydrocarbons. 

Oxygen traps are packed with a metal catalyst. Oxygen is 

the most detrimental contaminant to analytical columns. It 

produces irreversible oxidation damage particularly to polar 

stationary phases. Refer to Table 2 for a recommendation of 

what purifiers to use for various GC applications. 

Table 2—Gas Purifier Recommendations 

System Detector Gas Stream Recommended Purifier 

Packed Column GC 

All Carrier Gas Hydrocarbon, Moisture, Oxygen, 

and Indicating Oxygen 

Capillary Column GC 

All Carrier Gas Hydrocarbon, Moisture, Oxygen, 

and Indicating Oxygen 

ECD Make-Up Moisture and Oxygen 

FID Make-Up Hydrocarbon 

FID Air for FID Hydrocarbon 

FID Hydrogen for FID Hydrocarbon 

ELCD ELCD Reaction Gas Hydrocarbon 

Typical contaminants, such as moisture, oxygen, and 

hydrocarbons wreak havoc with GC columns and detectors. 

Capillary and packed columns can degrade when exposed 

to moisture or oxygen, particularly at high temperatures. 

Detector performance may also be compromised. Since the 

detector can actually “see” the contaminants, spikes, baseline 

noise, and drift result. 



Physical Characteristics 

You will also need to consider what physical characteristics 

your purifier should possess to meet your application 

requirements. Do you require 1/8" or 1/4" fittings, or are you 

performing bulk gas purification that requires 1/2" fittings? 

What are the pressure requirements of your application? This 

will determine if you need a purifier with a polycarbonate, 

glass, or metal body. Where will you be installing the purifier 

and is your installation space limited? This will determine the 

dimension and style of purifier you should choose. 

Carrier Gas 

Figure 1 — How to Set Up Carrier Gas Purifiers 

1. High Capacity or Indicating Moisture Trap 

2. High Capacity Hydrocarbon Trap 

1 

Carrier Gas Line 

2 3 4 

Gas Chromatograph 

Chemical 

Filter Supplied 

with GC 

Gas Purifier Installation Order 

3. Oxygen Trap 

(high capacity) 

4. Indicating Oxygen 

Trap 

4138 

Purifier Styles 

Grace offers two purifier styles—in-line and cartridge. The 

in-line style is the traditional style that is most commonly used. 

It can be directly installed into your gas lines with no support 

depending on its dimension or can be easily mounted on the 

wall with mounting clips. In-line purifiers must be removed 

from the gas line to be refilled or regenerated. We recommend 

installing them with quick-connect connectors and keeping an 

extra purifier on hand. 

The cartridge style purifier sits on the countertop for easy 

access when it is time to replace spent traps. The cartridge 

style can be installed in series with shut off valves allowing 

you to easily isolate them from your gas lines when it is 

time to replace them. Cartridge purifiers allow you to quickly 

remove the spent purifier from your gas line and replace it 

with a new one. 

Purifier Capacity 

Purifier capacity indicates the amount of contaminant a 

trap is capable of removing before it reaches its point of 

saturation. It is dependent on both the adsorbent’s capabilities 

and purifier’s volume. You need to consider the amount of 

impurities in your gas stream and the volume of gas you 

need to purify. If you are purifying a large volume of gas or 

a less pure gas, you should select a high capacity trap with 

a large volume adsorbent bed that will trap and retain more 

contaminants. 

Purifier Efficiency 

Purifier efficiency is the most important purifier characteristic. 

It indicates how effective the adsorbent is at removing the 

desired contaminant from your gas stream. If your application 

requires high purity levels or the lowest detection limits, select 

a purifier that is packed with a highly efficient adsorbent 

material that removes contaminants to ppb levels. 

The order in which you install purifiers into your gas lines is 

very important. Figure 1 demonstrates the correct order for 

installing purifiers into your carrier gas line. The moisture trap 

should be placed first in line. Both hydrocarbon and oxygen 

traps will adsorb water. Installing a moisture trap before these 

traps prevents them from becoming prematurely saturated 

with moisture. It also allows them to more effectively remove 

their specified contaminants from the gas stream. The 

hydrocarbon trap should be placed after the moisture trap. 

A high-capacity oxygen trap and an indicating oxygen trap 

should be placed closest to the GC. We recommend installing 

the series of purifiers as close to the GC as possible. 

Gas purifiers should be installed in a vertical position to 

prevent channeling. Channeling is a phenomenon that occurs 

when a purifier is installed in a horizontal position. The 

adsorbent bed settles and the gas stream passes over the top 

of the bed rather than through the bed. The gas stream does 

not sufficiently interact with the adsorbent bed to effectively 

remove impurities. 

Gas purification systems simplify purifier installation in to your 

gas lines. Systems are available as multi-adsorbent single 

purifiers or compact panels that contain multiple purifiers. 

Purifier Replacement/Regeneration 

You need to replace your gas purifiers before they are fully 

saturated. You can either change them at a predetermined 

time interval or use an indicating trap that changes color 

as it becomes saturated. There are potential problems with 

scheduled replacements. Undetected leaks or accidental 

exposure to air during cylinder changes can prematurely 

saturate gas purifiers. Early breakthrough means your column 

and detectors are exposed to a constant stream of damaging 

contaminants. An indicating trap located downstream from a 

high capacity trap is the best way to determine when it’s time 

to replace a spent trap. Some purifiers contain both the high 

capacity and indicating adsorbent in the same purifier body 

while others rely on a separate trap that serves solely as an 

indicator. Either way, purifier replacement should coincide with 

the first signs of color change in the indicating adsorbent. 



269



Moisture Traps 

Remove Moisture From Your Gas System 

• Eliminate stationary phase degradation 

• Increase column life 

• Improve the quality of your chromatography 

Remove moisture from gas cylinders, regulators and gas lines 

with a Grace moisture trap. In addition to H2O, most traps 

(except the ALL-Pure and Sodium Sulfate) also remove HCl, 

SO2, NO2, H2S, CO2, oil, and small aliphatics. Both indicating 

and non-indicating traps are available. Many traps can be 

regenerated and are refillable—order refill kits separately. 

Alltech ® Cartridge Style Moisture Trap 

A) Cartridge Style Moisture Trap 

– Simply replace the cartridge when spent 

– Gas flow stops while cartridge is changed 

– Order baseplate unit separately below 

In-Line Style Moisture Traps 

B) Alltech ® Standard Moisture Trap 

– Suitable for air lines 

– Refillable 

– SS screen at outlet prevents particulates from entering the 

gas stream 

C) Alltech ® Traditional Hydro-Purge Moisture Trap 

– Sorbsil ® Chameleon ® indicating material 

– SS screens at each end prevent particulates from entering 

the gas stream 

D) Alltech ® Hydro-Purge II Moisture Trap 

– 40µm SS frits in endfittings prevent particulates from entering 


– Larger capacities than the Traditional Hydro-Purge 

In-Line Style Moisture Traps (continued) 

E) Alltech ® Glass Moisture Trap 

– Best for carrier gas lines 

– Glass housing eliminates diffusion 



F) ALL-Pure Moisture Trap 

– Removes moisture down 

to


Oxygen Traps 

Protect Columns From Oxygen Contamination 

Prevent oxidation of column stationary phases with Grace 

oxygen traps. Use an oxygen trap to help maintain column 

performance and prolong column life. Traps are available in 

cartridge and in-line styles. Choose an indicating trap to easily 

identify when trap is spent or to use in conjunction with larger 

capacity traps. Some traps can be regenerated—see table on 

following page. 

Alltech ® Cartridge Style Oxygen Traps 

A) AT-Indicating Oxy Cartridge 

– Features the OXY-MAX adsorbent 

– Easy to replace—simply change cartridge 

– Gas flow stops while cartridge is charged 

– Order baseplate separately below 

B) AT-Omni Cartridge 

– Features the OXY-MAX adsorbent 

– Also removes moisture and hydrocarbons 

– Easy to replace—simply change cartridge 

– Gas flow stops while cartridge is charged 

– Order baseplate separately below 

Don’t forget the baseplate for cartridge 

style traps. Part No. 81025. 

In-Line Style Oxygen Traps 

C) Alltech ® Oxy-Trap 

– Features OXY-MAX adsorbent 

– Compact coil design 

D) Alltech ® Large Oxy-Trap 

– Same as original Oxy-Trap 

except larger capacity 

E) Alltech ® Indicating Oxy-Trap 

– Features OXY-MAX 

adsorbent 

– Glass housing eliminates diffusion 

F) Alltech ® Indicating Oxy-Purge ® 

– Glass housing 

eliminates diffusion 

– Endfittings contain 

40µm SS frits for particulate control 

G) Oxy-Purge ® N 

– Also removes moisture 

– Rugged aluminum 

housing 


gas stream 

H) ALL-Pure Oxygen Trap 

– Also removes 

moisture 

– Capacity of 1030mL 

of oxygen 

I) Oxi Clear Oxygen Trap 

– Removes oxygen, moisture, and 

hydrocarbons 

J) Alltech ® BIG Oxygen Trap 

– Our highest capacity 

trap—3000mL 


K) Alltech ® High-Pressure Oxy-Trap 

– Features OXY-MAX 

adsorbent 

– Handles pressures up 

to 1800psig 

Cartridge Oxygen Traps 

Pressure 

Limit Fitting Dimensions (L x o.d.) Indicator Part No. 

Regeneration Service 

Part No. 

Photo Description Capacity Efficiency 

Cartridge Style Traps 

A AT-Indicating Oxy 104mL



Hydrocarbon, CO2, and Sulfur Traps 

Eliminate Detector Noise and Increase 

Baseline Stability 

• Choose baseplate, cartridge, or in-line styles 

• Indicating hydrocarbon trap available 

Grace hydrocarbon traps adsorb trace amounts of 

hydrocarbons and organics from your gas stream. Most 

traps are packed with activated charcoal and adsorb organic 

compounds larger than methane. Traps are available in a 

baseplate style that sits on the benchtop, a cartridge style that 

allows for easy replacement of the trap (requires baseplate 

unit to be ordered separately), and an in-line style that is 

freely supported on gas lines. Traps for CO2, and sulfur are 

also available. 

Alltech ® Cartridge Style Traps 

A) Organic Cartridge Trap 

– Economical 

B) AT-Omni Cartridge Trap 

– Removes hydrocarbons, moisture, 

and oxygen 

– Indicating for oxygen 

A 

Don’t forget the baseplate for cartridge 

style traps. Part No. 81025. 

B 

In-Line Style Traps 

C) Alltech ® Activated Charcoal Trap 

– Two sizes available 

D) Hydrocarbon Trap 

– Economical 

– 250psig pressure rating 

E) Alltech ® Hi-EFF Organic Trap 

– Removes hydrocarbons and moisture 

– Indicating for moisture 

F) Alltech ® BIG Hydrocarbon Trap 

– Large 750cc size 

– Helium purged prior to shipment 

G) ALL-Pure Hydrocarbon Trap 

– Removes hydrocarbons to


Alltech ® Gas Purification 

Cartridge Systems 

• Replace spent purifiers without shutting down your system 

Alltech ® gas purification cartridges allow replacement of spent 

cartridges in seconds without shutting down your GC system. 

Simply pull the spent cartridge off the baseplate and push a 

new one into place. 

A knurled locking 

ring keeps the 

cartridge locked 

in place. Four 

different purification 

cartridges are 

available. Multiple 

cartridges and 

baseplates can be 

plumbed together 

to customize a gas 

Knurled 

locking ring 

Convenient cartridge baseplate 

automatically stops gas flow 

when changing cartridges 

purification system for your application. Order each cartridge 

separately below. 

Moisture Trap Cartridge—Filled with indicating Sorbsil ® 

Chameleon ® indicating material and molecular sieve pellets to 

maximize moisture removal and to indicate when the cartridge 

requires refilling or replacement. A refill kit is available below. 

Organic Trap Cartridge—Removes non-methane 

hydrocarbons from your gas stream. A refill kit is available 

below. 

AT-Omni Cartridge—A multi-purpose cartridge filled with 

OXY-MAX and Hi-EFF Charcoal to effectively remove 

oxygen, moisture and hydrocarbons. AT-Omni cartridges 

are disposable. 

AT-Indicating Oxy Cartridge—Filled with OXY-MAX and 

indicating oxygen adsorbent to efficiently remove oxygen and 

small amounts of moisture. The indicating material shows a 

color change when the cartridge is saturated. AT-Indicating 

Oxy cartridges are disposable. 

ALL-Pure Gas Purifiers 

• One purifier removes multiple contaminants 

• Reduce impurities to low ppb levels 

ALL-Pure Gas Purifiers feature a unique proprietary 

broad spectrum adsorbent material that removes multiple 

contaminants with a single purifier. Replace three separate 

purifiers for oxygen, moisture, and hydrocarbons with a single 

ALL-Pure purifier. Purifiers are gas specific or choose 

purifiers specific for moisture, hydrocarbons, or oxygen. 

ALL-Pure Specifications 

Dimensions: 400cc, 21" L x 1.5" o.d. (50.8cm x 3.8cm) 

Max Flow: 500mL/min 


Max. Temp: 80°C 

Mounting: Purifier must be mounted in vertical position 

Purifier Selectivity and Purity Specifications* 

Purifier CO CO2 O2 H2O 

Sulfur 

Compounds 

Non-Methane 

Hydrocarbons 

Helium Purifier:


SGT Super Clean Filters 

Improve Baselines, Minimize Interferences, 

and Reduce MS Maintenance 

• Remove impurities to deliver 99.9999% pure gas 

• Exchange filters in seconds, without introducing air 

into the system 

• Diffusion-proof with glass and metal construction 

• Pressure resistant up to 150psig (11 bar) 

These SGT Super Clean filters can be used on all of your 

GC gas lines to deliver 99.9999% pure gas. Filters are 

available for oxygen, moisture and hydrocarbons. The 

Combi filter removes both moisture and hydrocarbons. The 

Triple filter removes moisture, oxygen, and hydrocarbons and 

is available purged with Argon for standard applications or 

purged with Helium for MS applications. 


Filters feature a quick change baseplate/cartridge design. 

Cartridges can be exchanged within seconds, during analysis, 

and without tools. 1-, 2-, 3-, or 4-position baseplates are 

available and will accept any of the GC filter cartridges. 

Baseplates must be ordered separately (except for systems). 

Filter cartridge bundles include the most popular filters in 

one package. Various filter systems are available and include 

the appropriate baseplate. 

SGT Super Clean Filter Specifications 

Maximum Flow: 

7.0L/min 

Efficiency: 


Purifier Accessories 

Universal Mounting Panel 

• Mount up to five purifiers for easy monitoring 

and replacement 

• Pre-drilled for easy mounting 

• Fits most manufacturers’ purifiers 

This lightweight aluminum mounting panel will hold up to five 

purifiers. Pre-drilled holes are spaced at 3/4" centers in five 

vertical rows to allow for maximum versatility. Mounting clips 

to accommodate purifiers from 7/8" to 2-1/2" o.d. (2.2cm 

to 6.4cm) are sold separately below. Panel measures 

17" W x 24" H (43.2cm W x 61cm H). 

In-Line Particulate Filter 

• Economically remove particulates from gas streams 

This brass in-line particulate filter is a convenient, inexpensive 

way to remove particulates from critical gas streams. Each 

unit includes one 10 micron filter cartridge. The maximum 

pressure rating is 500psig. 

4858 

In-Line Particulate Filters 

Description 

Part No. 

1/4" In-line Brass Filter 8052 

1/8" In-line Brass Filter 8054 

4854 


Need a leak detector? 

Ensure leak-free installation of your gas 

purifiers. See page 267 for gas leak 

detectors. 






Universal Mounting Panel 


Universal Mounting Panel ea 7220 

Universal Mounting Clips 

Fits 7/8" to 1" o.d. Traps 2/pk 7221 

Fits 1-1/8" to 1-3/8" o.d. Traps 2/pk 7223 



Fits 2" to 2-1/2" o.d. Traps 2/pk 7229 


275


Gas Sampling Bags 

Selection Guides 

FEP Gas Sampling Bags, 5mil 

Tensile Strength* 

Fittings Available (by ASTM D-882) 

Barbed on/off 

Septum Port 

Dual 

1/4" SS Fitting 

2N1 Combination 

2500–3000psig 

(Note: This is NOT 

a burst pressure 

rating for the bag.) 

Permeability 

Gas 

CO2 

Hydrogen 

Nitrogen 

Oxygen 

Acetic Acid 

Vapor 

Acetone 

Benzene 

CCl4 

Ethanol 

Hexane 

Water 

Permeability** 

1670 

2200 

320 

750 

0.41 

Permeability*** 

0.95 

0.64 

0.31 

0.69 

0.56 

0.45 

Temp. Limits 

min.–max. Sample Compatibility Advantage 

-240–260°C 

Inert and solvent resistant 

to virtually all chemicals 

except molten alkali metals, 

gaseous fluorine, complex 

halogenated compounds 

at elevated pressure and 

temperature 

Low permeability to liquids, 

gases, moisture and organic 

vapors; suitable for 

cryogenic use 

gc accessories | gas sampling 

Tedlar ® PVF, 2mil 

Fittings Available 

Barbed on/off 

Septum Port 

Dual 

1/4" SS Fitting 



(by ASTM D-882) Permeability 

Gas 

17,000–18,000psig 

(Note: This is NOT 

a burst pressure 

rating for the bag.) 

CO2 

Hydrogen 

Nitrogen 

Oxygen 

Vapor 

Acetic Acid 

Acetone 

Benzene 

CCl4 

Ethyl Acetate 

Ethanol 

Hexane 

Water 

Permeability** 

11.1 

150 

0.25 

3.2 

Permeability*** 

45 

10,000 

90 

50 

1.000 

35 

55 

180 

Temp. Limits 

min.–max. Sample Compatibility Advantage 

-234–300°C 

Cali-5-Bond 5.5mil (polyethylene, polyamide, aluminum foil, polyvinyl, chloride polyester) 

Fittings Available 


Barbed on/off 

Septum Port 


(by ASTM D-882) 

Recommended for analysis 

of non-corrosive gases, 

TCLP gases, CO, H2S, 

radon, and mercaptans; 

“Grab” sampling; preparation 

of calibration gas standards; 

gas blending; stack 

sampling; emission testing 

Temp. Limit 

max. Sample Compatibility Advantage 

Permeability 

— — 54°C Designed specifically for 

collecting air samples; 

suitable for the storage 

and transportation of 

gas samples 

*Tensile strength is the maximum force required to rupture a one-inch wide specimen. **cc/(100in 2 )(24hrs)(atm/mil). ***gms/(100in 2 )(24hrs/mil). 

Lowest permeability of all 

types of bags; economical 

alternative to PTFE; easily 

cleaned and reused 

Opaque bag protects sample 

from UV degradation; bags 

may be purged with nitrogen 

or pure air and reused 

Available Fittings 

Grace offers a large variety of gas sampling bag fittings. Consider the following when selecting a fitting: 

1. How will you collect the sample? What type of connection is available to connect to the bag? 

2. How will you need to access the sample once it is collected in the bag? 

3. Is your sample compatible with the fitting material? 

Nickel-plated Barbed 

On-Off Valve 

6644 6642 6738 6645 6643 

PTFE Septum Port Dual On-Off/Septum 1/4" SS Swagelok ® 2N1 Combination 

On-Off/Septum 

more info 

Need a syringe? 

See pages 336–347 for our complete selection of syringes. 




Tedlar ® Gas Sampling Bags 

• High tensile strength resists puncture 

• Low permeability minimizes sample loss 

Tedlar ® bags are manufactured from sturdy 2mil PVF film that is inert to 

a wide range of gases. Low permeability ensures the integrity of your 

sample and makes Tedlar ® bags the ideal choice for off site analysis. 


Barbed On-Off Valve 

Part No. 

Septum Port 

Part No. 

Dual On-Off/Septum Port 

Part No. 

1/4" SS Swagelok ® 

Part No. 

Nominal Size 

Volume 

6" x 6" 0.6L 41021 41023 41025 41027 

6" x 10" 1.2L 41029 41031 41033 41035 

6" x 12" 1.5L 41037 41039 41041 41043 

9" x 9" 2L 41045 41047 41049 41051 

12" x 12" 5L 41053 41055 41057 41059 

12" x 19" 10L 41063 41065 41067 41069 

18" x 18" 16L 41071 41073 41075 41077 

24" X 24" 40L 41079 41081 41083 41085 

24" x 30" 56L 41087 41089 41091 41093 

24" x 36" 73L 41095 41097 41099 41032 

30" x 30" 80L 41034 41036 41038 41042 

FEP Gas Sampling Bags 

• 5mil thickness for durability 

• Manufactured and packaged under GLP conditions 

FEP bags have a 5mil film thickness making them suitable for harsh 

sampling conditions. Bags are chemically resistant to the most severe 

corrosives. Choose from four different port types. 


Barbed On-Off Valve 

Part No. 

Septum Port 

Part No. 

Dual On-Off/Septum 

Port Part No. 

1/4" SS Swagelok ® 

Part No. 

Nominal Size 

Volume 

6" x 6" 0.6L 41044 41046 41048 41052 

6" x 10" 1.2L 41054 41056 41058 41064 

6" x 12" 1.5L 41066 41068 41072 41074 

9" x 9" 2L 41076 41078 41082 41084 

12" x 12" 5L 41086 41088 41092 41094 

12" x 19" 10L 41106 41108 41109 41125 

18" x 18" 16L 41127 41129 41131 41133 

24" x 24" 40L 41135 41137 41139 41149 

24" x 30" 56L 41151 41153 41155 41157 

24" x 36" 73L 41126 41128 41132 41134 

30" x 30" 80L 41136 41138 41152 41154 

6647 

6646 



Replacement Parts for Tedlar ® and FEP Bags 

Description 

Part No. 

Replacement Septa for Septa Port, 10/pk 41158 

1/4" PTFE No Hole Ferrules for 1/4" SS Fitting, 10/pk RF-400/0-T 


277



Combo Valve Gas Sampling Bags 

• On/off valve and septum in one fitting 

• Save with convenient multi-packs 

Cali-5-Bond 5-Layer Gas Sampling Bags 

• Non-permeable 

• Opaque to UV and visible light 

• Inert, flexible, tough, and reusable 

best 

seller 

6648 1651 


Combo valve gas sampling bags give the convenience and 

ease of a barbed on/off valve and a septum port combined 

into one fitting. The valve fitting features a 3/16" stem, which 

is suitable for use with most 1/4" tubing, and a sampling port. 

Bags are available in Tedlar ® and FEP as well as convenient 

multi-packs. 

Combo Valve Gas Sampling Bags 

Size Volume Port Description Part No. 


7 x 7" 1L 2N1 41001 

9 x 9" 2L 2N1 41003 

12 x 12" 5L 2N1 41007 

12 x 19" 10L 2N1 41011 

18 x 24" 25L 2N1 41017 


6 x 6" 0.6L 2N1 41002 

6 x 10" 1.2L 2N1 41004 

6 x 12" 1.5L 2N1 41006 

9 x 9" 2L 2N1 41008 

Tedlar ® Combo Valve Multi-Packs 

Size Volume Port Description Qty. Part No. 

7 x 7" 1L 2N1 10 48001 

9 x 9" 2L 2N1 10 48003 

12 x 12" 5L 2N1 10 48007 

12 x 19" 10L 2N1 10 48011 

18 x 24" 25L 2N1 5 48017 

Cali-5-bond gas sampling bags are constructed from 

inert 5.5mil thick material made from five layers of film. 

The layers are comprised of an inner layer of high-density 

polyethylene, followed by polyamide, an aluminum foil barrier, 

a polyvinylidene chloride layer and an outer layer of polyester. 

These bags are specifically designed for air sampling and are 

suitable for the storage and transportation of gas samples. 

Cali-5-Bond bags are available in both pillow and snout 

shapes. The snout provides additional entry into the bag for 

flushing and sample collection. 

Bags are equipped with a barbed on-off valve and silicone 

septum for easy sampling and can be purged with nitrogen 

or pure air gas and reused. 

5-Layer Gas Sampling Bags 

Nominal Size Volume Port Description Part No. 

Snout-Shaped Gas Sampling Bags 

8 x 15" 2L Barbed Valve 1" Septa 4150 




Pillow-Shaped Gas Sampling Bags 







Gas Sampling Accessories 

Gas Sampling Bulb with Septum Ports 

• PTFE stopcocks for increased compatibility 

Gas Sampling Bulbs 

Description 

Part No. 

125mL, PTFE Stopcock 7012 




Replacement Septa, 12/pk 7000 

Gas Sampling Bulb with Twist-Lock Stopcocks 

• Unique twist-lock stopcocks 

• PTFE outlet tube connectors 

Gas Sampling Bulb with Injection Port and 

Valve Stopcocks 

• Stored sample contacts only glass and PTFE 

• Suitable for vacuum or pressure 

• Semi-needle valve control 

4663 

4664 

best 

seller 


Description 

Part No. 





Vacuum Sampler 

• No external pump required 

• Reusable 

These evacuated 1L vacuum samplers are fitted 

with a unique septum port and needle guide 

through which the container is filled and samples 

removed. A 4" long needle is supplied with each 

vacuum sampler (2 needles with the package of 

12 samplers). 

Vacuum Sampler 

Description 

Part No. 

1L (1 needle), ea 8020 

1L (2 needles), 12/pk 8022 


Gas and Vapor Sampler 

• Sample bags are removable and resealable 

Collect samples in resealable 200cc multilayer bags. 

Simply evacuate the canister using the supplied 

aspirator bulb. The bags can be removed for storage 

or shipment, freeing the canister for further sampling. 

Ten bags are included with each kit. 

Gas and Vapor Sampler 

Description 

Part No. 

Gas and Vapor Sampler Kit 4182 

Replacement Bags, 10/pk 4184 


Looking for syringes? 


6310 

4668 


4665 


Description 

Part No. 

125mL 6940 

250mL 6942 

500mL 6944 

13-425 Open-Hole Caps, 100/pk 73090 

TFE/Silicone Liners, 100/pk 98123 


279


GC Ferrule Selection 

PTFE Ferrules 

PTFE ferrules are completely inert and an economical choice. 

They have an upper temperature limit of 250°C. PTFE ferrules 

conform well to the shape of the column upon compression 

and can be reused if handled carefully. 

Graphite Ferrules 

These 99.95% pure graphite ferrules can be used at 

temperatures up to 450°C without producing bleed or 

decomposition products. The one-piece design requires no 

back ferrules. Graphite ferrules are very soft and conform well 

to the column on compression. These ferrules can be reused 

as long as care is taken not to overtighten them. 

Vespel ® Ferrules 

Vespel ® ferrules do not cold flow, are easily reusable, and 

withstand temperature up to 350°C. At high temperatures, 

Vespel ® may adhere to glass or metal. 

Vespel ® /Graphite Ferrules 

Composites of Vespel ® and graphite combine the advantages 

of the two materials. They are less likely to adhere to the 

column than Vespel ® but are more durable than graphite. 

Ferrules are easy to reuse and stable at temperatures to 

400°C. 

Two types of composites are available. The VG1 composite 

is 85% Vespel ® /15% graphite and suitable for all-purpose 

applications. The VG2 type is 60% Vespel ® /40% graphite and 

exhibits added lubricity. 

gc accessories | fittings 

Ferrule Properties 

Material Temp. Limit Reusability Resealing Properties Suitability for Glass Temp. Prog. 

PTFE 250°C Good Excellent Excellent No 

Graphite 450°C Good Excellent Excellent Yes 

Vespel ® 350°C+ Excellent Good Good Yes 

VG1 400°C Excellent Good Excellent Yes 

VG2 400°C Excellent Good Excellent Yes 

ptfe and Graphite Ferrules 

Description Fitting Size Tubing Size Qty. 

Straight Ferrules 

Used when 

the tubing o.d. 

matches the 

nominal size 

of the fitting 

being used. 

Reducing Ferrules 

Used when 

the tubing o.d. 

is smaller than 

the nominal size 

of the fittings. 

PTFE 

Part No. 

What ferrule type do you need? 

Ferrule Type Use for: 

0.4mm Hole: Capillaries with i.d. of 0.10-0.25mm 



NO-HOLE: As plugs, or drill for special needs 

2-HOLE: Connecting two pieces of capillary tubing 

or columns to the same endfitting 

Graphite 

Part No. 

1" 1" 2 — 14361 

7/8" 7/8" 2 — 14360 

3/4" 3/4" 2 — 14358 

5/8" 5/8" 2 — 14357 

1/2" 1/2" 10 — SF-800-G 

3/8" 3/8" 10 SF-600-T SF-600-G 

1/4" 1/4" 10 SF-400-T SF-400-G 

3/16" 3/16" 10 SF-300-T SF-300-G 

1/8" 1/8" 10 SF-200-T SF-200-G 

1/16" 1/16" 10 SF-100-T SF-100-G 

1/4" 1/16" 10 RF-400/100-T RF-400/100-G 

1/4" 0.8mm 10 — RF-400/0.8-G 

1/4" 1/8" 10 RF-400/200-T RF-400/200-G 

1/4" 3/16" 10 RF-400/300-T 14327 

1/4" 4mm 10 — 14384 

1/4" 6mm 10 RF-400/6MM-T RF-400/6MM-G 

1/8" 1/16" 10 RF-200/100-T RF-200/100-G 

1/8" 1.2mm 10 — 14326 

1/8" 1.0mm 10 — 14311 

1/8" 0.8mm 10 — RF-200/0.8-G 

1/8" 0.5mm 10 — RF-200/0.5-G 

1/8" 0.4mm 10 — RF-200/0.4-G 

1/8" 1.2mm 10 — 14305 

1/16" 1.0mm 10 — RF-100/1.0-G 

1/16" 0.8mm 10 — RF-100/0.8-G 

1/16" 0.5mm 10 — RF-100/0.5-G 

1/16" 0.4mm 10 — RF-100/0.4-G 

1/16" 0.3mm 10 — 14308 



GC Ferrules (continued) 

PTFE and Graphite Ferrules (continued) 


HP/Agilent Short Ferrules 

Two-Hole Ferrules 

No-Hole Ferrules 

Graphite Ferrules for HP/Agilent Nuts 

Vespel ® and Vespel ® /Graphite Ferrules 


Straight Ferrules 

Reducing Ferrules 

No-Hole Ferrules 

Two-Hole Ferrules 

Vespel ® 

Part No. 

PTFE 

Part No. 

VG1 

Part No. 

Graphite 

Part No. 

1/16" 1.2mm 10 — 14370 

1/16" 1.0mm 10 — 14325 

1/16" 0.8mm 10 — 14324 

1/16" 0.5mm 10 — 14323 

1/16" 0.4mm 10 — 14322 

1/16" 0.3mm 10 — 14321 

1/8" 0.8mm 10 — 14334 

1/8" 0.5mm 10 — 14307 

1/16" 0.5mm 10 — 14330 

3/8" — 10 — 14332 

1/4" — 10 RF-400/0-T 14328 

1/8" — 10 RF-200/0-T 14314 

1/16" — 10 RF-100/0-T 14310 

0.5mm — — 10 — 1547901 

1.0mm Hole — — 10 — 1548001 

VG2 

Part No. 

1/4" 1/4" 10 SF-400-V SF-400-VG1 SF-400-VG2 

1/4" 1/4" 10 15449* 15449* 15451* 

1/8" 1/8" 10 SF-200-V SF-200-VG1 SF-200-VG2 

1/8" 1/8" 10 — 15450* 15452* 

1/16" 1/16" 10 SF-100-V SF-100-VG1 SF-100-VG2 

1/4" 6mm 10 RF-400/6MM-V 400/6MM-VG1 400/6MM-VG2 

1/4" 3/16" 10 RF-400/300-V 400/300-VG1 400/300-VG2 

1/4" 1/8" 10 RF-400/200-V 400/200-VG1 400/200-VG2 

1/4" 1/16" 10 RF-400/100-V 400/100-VG1 400/100-VG2 

1/4" 0.8mm 10 — 400/0.8-VG1 400/0.8-VG2 

1/8" 1/16" 10 RF-200/100-V 200/100-VG1 200/100-VG2 

1/8" 0.8mm 10 — 200/0.8-VG1 200/0.8-VG2 

1/8" 0.5mm 10 — 200/0.5-VG1 200/0.5-VG2 

1/8" 0.4mm 10 — 200/0.4-VG1 200/0.4-VG2 

1/16" 1.0mm 10 RF-100/1.0-V 100/1.0-VG1 100/1.0-VG2 

1/16" 0.9mm 10 RF-100/0.9-V 100/0.9-VG1 100/0.9-VG2 

1/16" 0.8mm 10 RF-100/0.8-V 100/0.8-VG1 100/0.8-VG2 

1/16" 0.5mm 10 RF-100/0.5-V 100/0.5-VG1 100/0.5-VG2 

1/16" 0.4mm 10 RF-100/0.4-V 100/0.4-VG1 100/0.4-VG2 

1/16" 0.3mm 10 — 100/0.3-VG1 — 

1/4" — 10 RF-400/0-V 400/0-VG1 400/0-VG2 

1/8" — 10 RF-200/0-V 200/0-VG1 200/0-VG2 

1/16" — 10 RF-100/0-V 100/0-VG1 100/0-VG2 

1/16" 0.5mm 10 — 15486 15488 

1/16" 0.4mm 10 — 15481 15487 


*Equivalent to Parker one-piece metal ferrule. 


281


Fittings Selection Guide 

To determine the size of a nut or female pipe thread, compare it to the silhouettes below. To determine the size of a ferrule, 

measure its inside diameter. Please note that tubing fitting sizes are based on the outside diameter of the tube, while pipe thread 

fitting sizes are based on the inside dimension of the pipe. Pipe and tubing fittings are not interchangeable. 

Tubing Nut Sizes* 

1/16" 1/8" 3/16" 1/4" 3/8" 1/2" 

0127 

Female Pipe Thread Connectors* 

Tubing 


Tubing 

*Silhouettes shown at actual size. 

Parker Fittings 

1/8" FPT 

1/8" (6-2-GBG) 1/4" FPT 3/8" FPT 1/2" FPT 

Parker fittings are available in two forms: the CPI one-piece ferrule and the A-Lok two-piece ferrule. Most parts are available in 

brass or stainless steel and should be matched to the tubing, particularly in thermal applications. 

5767 

Parker Fittings 

Description Dimensions Qty. 

Nuts 

Ferrules 

CPI 

Tees 

A-LOK 

A-Lok 

Brass 

Part No. 

CPI 

Brass 

Part No. 

Qty. 

A-Lok 


Part No. 

CPI 


Part No. 

1/16" 10/pk 11600 14057 10/pk 11700 141575 

1/8" 10/pk 11601 14058 10/pk 11701 14158 

3/16" 10/pk 11602 60000 10/pk 11702 61000 

1/4" 10/pk 11603 14059 10/pk 11703 14159 

3/8" 10/pk 11604 60002 10/pk 11704 610025 

1/2" 10/pk 11605 600045 5/pk 11705 610045 

1/16" 10/pk 11606 14060 10/pk 11706 141605 

1/8" 10/pk 11607 14061 10/pk 11707 14161 

3/16" 10/pk 11608 60006 10/pk 11708 61006 

1/4" 10/pk 11609 14062 10/pk 11709 14162 

3/8" 10/pk 11610 60008 10/pk 11710 610085 

1/2" 5/pk 11611 600105 5/pk 11711 610105 

1/16" ea 11612 14075 ea 11712 14175 

1/8" ea 11613 14053 ea 11713 14176 

3/16" ea 11614 60012 ea 11714 61012 

1/4" ea 11615 14077 ea 11715 14177 

3/8" ea 11616 60014 ea 11716 61014 

1/2" ea 11617 60016 ea 11717 61016 



Parker Fittings (continued) 

Parker Fittings (continued) 


Unions 

Reducing Unions 

Capillary Union 

A-Lok 

Brass 

Part No. 

CPI 

Brass 

Part No. 

Qty. 

A-Lok 


Part No. 

CPI 


Part No. 

1/16" ea 11618 14063 ea 11718 14163 

1/8" ea 11619 14065 ea 11719 14165 

3/16" ea 11620 60018 ea 11720 61018 

1/4" ea 11621 14068 ea 11721 14168 

3/8" ea 11622 60020 ea 11722 61020 

1/2" ea 11623 60022 ea 11723 61022 

1/8" to 1/16" ea 11624 14064 ea 11724 14164 

3/16" to 1/8" ea 11625 60024 ea 11725 61024 

1/4" to 1/16" ea 11626 60026 ea 11726 14166 

1/4" to 1/8" ea 11627 60028 ea 11727 61028 

1/4" to 3/16" ea 11628 14067 ea 11728 14167 

3/8" to 1/16" ea — — ea 11729 14174 

3/8" to 1/4" ea 11630 60030 ea 11730 61030 

1/16" ea — — ea — 28812 

Female Pipe Thread Connectors (Tube to FPT) 

1/8" to 1/8" FPT ea 11800 60048 ea 11750 61048 

1/8" to 1/4" FPT ea 11801 14071 ea 11751 61084 

1/4" to 1/8" FPT ea 11802 60050 ea 11752 61050 

1/4" to 1/4" FPT ea 11803 14072 ea 11753 61086 

3/16" to 1/8" FPT ea 11804 60052 ea 11754 61052 

3/8" to 1/8" 6-2-GBZ ea 11805 60054 ea 11755 61054 

3/8" to 1/4" FPT ea 11806 60056 ea 11756 61056 

Male Pipe Thread Connectors (Tube to MPT) 

1/16" to 1/16" MPT ea 11632 60034 ea 11732 61034 

1/16" to 1/8" MPT ea 11633 60036 ea 11733 61036 

1/8" to 1/8" MPT ea 11634 60038 ea 11734 61038 

1/8" to 1/4" MPT ea 11635 60039 ea 11735 61039 

3/16" to 1/8" MPT ea 11636 60040 ea 11736 61040 

1/4" to 1/8" MPT ea 11637 60042 ea 11737 61042 

3/8" to 1/8" MPT ea 11638 60044 ea 11738 61044 

1/4" to 1/4" MPT ea 11639 14069 ea 11739 61082 

3/8" to 1/4" MPT ea 11640 60046 ea 11740 61046 

Tube End Connectors (Reducers) 

1/16" to 1/8" tube ea 11807 60058 ea 11757 61058 

1/16" to 1/4" tube ea — — ea 11758 61059 

1/8" to 1/4" tube ea 11809 60060 ea 11759 61060 

1/8" to 3/16" tube ea 11810 60062 ea 11770 61062 

3/16" to 1/4" tube ea 11811 4052 ea 11771 61064 

1/4" to 1/8" tube ea 11812 14073 ea 11772 14169 

1/4" to 3/8" tube ea 11813 60066 ea 11773 61066 

3/8" to 1/4" tube ea 11814 60068 ea 11774 61068 

Caps 

1/16" ea 11815 14047 ea 11835 14151 

1/8" ea 11816 14051 ea 11836 14152 

3/16" ea 11817 60070 ea 11837 61070 

1/4" ea 11818 14052 ea 11838 14153 

3/8" ea 11819 60072 ea 11839 61072 

1/2" ea 11820 60074 ea 11840 61074 

Plugs 

1/16" ea 11821 14054 ea 11841 14154 

1/8" ea 11822 14055 ea 11842 14155 

3/16" ea 11823 60076 ea 11843 61076 

1/4" ea 11824 14066 ea 11844 14156 

3/8" ea 11825 60078 ea 11845 61078 

1/2" ea 11826 60080 ea 11846 61080 



283


Swagelok ® Tube Fittings 

Swagelok ® tube fittings provide a leak-proof, torque-free 

seal at all tubing connections and eliminate costly, hazardous 

leaks in instrumentation and process tubing. 

tech tip 

CAUTION: Do not mix or interchange parts of tube fittings made 

by other manufacturers with Swagelok ® tube fitting parts. 

All the action in the fitting moves along the tube axially instead 

of with a rotary motion. Since no torque is transmitted from 

the fitting to the tubing, there is no initial strain which might 

weaken the tubing. 

Swagelok ® Fittings 


Nuts 

Brass 

Part No. 

Qty. 


Part No. 

1/16" 10 55002 10 55199 

1/8" 10 55003 10 55193 

1/4" 10 55005 10 55195 

Male Nut 

1/16" ea 55084 ea 55184 


Front Ferrules 

Back Ferrules 

Union (Low Dead Volume) 

Unions 

1/16" 10 55006 10 55196 

1/8" 10 55007 10 55197 

1/4" 10 55013 10 55093 

1/16" 10 55014 10 55094 

1/8" 10 55015 10 55095 

1/4" 10 55017 10 55097 

1/16" ea 55001 ea 55101 

1/16" ea 55009 ea 55109 

1/8" ea 55010 ea 55110 

1/4" ea 55012 ea 55112 

Bulkhead Unions 

Reducing Unions 

1/16" ea 51017 ea 55117 

1/8" ea 55018 ea 55118 

1/4" ea 55020 ea 55120 

1/8" to 1/16" ea 55021 ea 55121 

3/16" to 1/16" ea 55022 ea 55122 

3/16" to 1/8" ea 55023 ea 55123 

1/4" to 1/16" ea 55024 ea 55124 

1/4" to 1/8" ea 55025 ea 55125 



Swagelok ® Fittings (continued) 

Swagelok ® Fittings (continued) 

Brass 

Part No. 


Part No. 


Qty. 

Female Pipe Thread Connectors (Tube to FPT) 

1/8" to 1/4" FPT ea 55047 ea 55147 

3/16" to 1/8" FPT ea 55048 ea 55148 

1/4" to 1/8" FPT ea 55049 ea 55149 

1/4" to 1/4" FPT ea 55050 ea 55150 

1/4" to 1/2" FPT ea 55052 ea 55152 

Male Pipe Thread Connectors (Tube to MPT) 

1/16" to 1/16" MPT ea 55033 ea 55133 

1/16" to 1/8" MPT ea 55034 ea 55134 

1/8" to 1/16" MPT ea 55035 ea 55135 

1/8" to 1/8" MPT ea 55036 ea 55136 

1/8" to 1/4" MPT ea 55037 ea 55137 

1/8" to 3/8" MPT ea 55038 ea 55138 

1/4" to 1/8" MPT ea 55042 ea 55142 

1/4" to 1/4" MPT ea 55043 ea 55143 

1/4" to 3/8" MPT ea 55044 ea 55144 

1/4" to 1/2" MPT ea 55045 ea 55145 

Tees 

1/16" ea 55027 ea 55127 

1/8" ea 55028 ea 55128 

Crosses 

Caps 

Plugs 

1/4" ea 55030 ea 55130 

1/8" ea 55031 ea 55131 

1/4" ea 55032 ea 55132 

1/16" ea 55085 ea 55185 

1/8" ea 55086 ea 55186 

1/4" ea 55088 ea 55188 

1/16" ea 55089 ea 55189 

1/8" ea 55090 ea 55190 

1/4" ea 55092 ea 55192 



Need tubing? 

See pages 384–391 for a complete 

selection of stainless steel, metal, and 

polymeric tubing. 






285


gc accessories | injector 

Deactivated Injection Liners 

Deactivated Injection Liners 

Description and Length x o.d. 

Injection Liners for Agilent Instruments* 

Cup Splitter, 

78.5 x 6.3mm 

Split/Splitless with Deactivated Quartz Wool, 

78.5 x 6.3mm o.d., 4mm i.d. 

Splitless, 

78.5 x 6.3mm o.d., 4mm i.d. 

Quartz Splitless, 

78.5 x 6.0mm, 2mm i.d. 

Recessed Gooseneck with Deactivated Quartz Wool, 

78.5 x 6.3mm, 4mm i.d. 

Recessed Gooseneck, 

78.5 x 6.3mm, 2mm i.d. 

Direct, 

78.5 x 6.3mm, 1.5mm i.d. 

Injection Liners for Varian ® Instruments 

Splitter with Deactivated Quartz Wool, 1075/1077, 

72 x 6.3mm, 4mm i.d. 

Frit Splitter, 1075/1077, 

72 x 6.3mm, 4mm i.d. 

Baffle Splitter, 1075/1077, 

72 x 6.3mm, 4mm i.d. 

Splitless 1075/1077, 

74 x 6.3mm, 2mm i.d. 

0.8mm SPI 1093/1094, 

54 x 4.6mm 

0.5mm SPI 1093/1094, 

54 x 4.6mm 

Injection Liners for PerkinElmer ® Instruments 

Split/Splitless Autosystem XL (PSS), 

86.2 x 4mm, 2mm i.d. 

Split/Splitless Autosystem, 

92 x 5.8mm, 4mm i.d. 

Injection Liners for Shimadzu ® Instruments 

Split 17A with Deactivated Quartz Wool, 

95.5 x 5.0mm, 3mm i.d. 

Splitless 17A, 

95.5 x 5.0mm, 3mm i.d. 

Injection Liners for Carlo Erba-Fisons Instruments 

Split/Splitless 6000 Series with Deactivated Quartz Wool, 

79.5 x 5.0mm, 3mm i.d. 

Splitless 6000 Series, 

79.5 x 5.0mm, 4mm i.d. 

Splitless 6000 Series, 

79.5 x 5.0mm, 2mm i.d. 

Split 8000 Series, 

105 x 8.0mm, 3mm i.d. 

best 

value 

Diagram 

5/pk 

Part No. 

25/pk 

Part No. 

4827 4829 

4924 4928 

4934 4936 

4963 4964 

4960 4961 

4938 4957 

4968 4969 

4971 4972 

4974 4975 

4978 4979 

4981 4982 

4984 4985 

4987 4988 

8618810 — 

4798 4800 

4750 4752 

4756 4758 

4744 4746 

4854 4855 

4851 4852 

4786 4788 


Don't forget septa! 

See pages 289–292 for a complete selection of septa. 



Deactivated SGE FocusLiners 

Deactivated SGE FocusLiners 

Description and Length x o.d. 

for Agilent Instruments* 

FocusLiner, 

78.5 x 6.3 x 4mm i.d. 

Tapered FocusLiner, 

78.5 x 6.3 x 4mm i.d. 

FAST FocusLiner, 

78.5 x 6.3 x 2.3mm i.d. 

Tapered FAST FocusLiner, 

78.5 x 6.3 x 2.3mm i.d. 

for Varian ® Injector Models 1075/1077 

FocusLiner, 

72 x 6.3 x 4mm i.d. 


74 x 6.3 x 4mm i.d. 

FocusLiner w/Top Restriction, 

72 x 6.3 x 4mm i.d. 

FAST FocusLiner, 

72 x 6.3 x 2.3mm i.d. 

for Varian ® Injector Models 1078/1079 

FocusLiner, 

54 x 5 x 3.4mm i.d. 


54 x 5 x 3.4mm i.d. 

for Shimadzu ® 17A 

FocusLiner, 

95 x 5 x 3.4mm i.d. 


95 x 5 x 3.4mm i.d. 

for Shimadzu ® 14/15A/16 

FocusLiner, 

99 x 5 x 3.4mm i.d. 


99 x 5 x 3.4mm i.d. 

for PerkinElmer ® Autosystem 

FocusLiner, 

86.2 x 4 x 2mm i.d. 

FocusLiner, 

92 x 6.2 x 4mm i.d. 


92 x 6.2 x 4mm i.d. 

for ThermoQuest Model 8000/TRACE 

FocusLiner, 

105 x 8 x 5mm i.d. 


105 x 8 x 5mm i.d. 

*Also compatible with Varian ® model CP-1177 Split/Splitless injection port. 

Diagram 

5/Pk 

Part No. 

25/Pk 

Part No. 

85474 85480 

85481 85574 

85605 85606 

85607 — 

85477 — 

85484 — 

85633 — 

85634 — 

85437 — 

85635 — 

85476 — 

85483 — 

85608 — 

85630 — 

8618809 — 

85478 — 

85482 — 

85631 — 

85632 — 







287



GC O-rings 

• Thermally stable 

• No bleed 

• Suitable for GC/MS 

Graphite O-rings may replace the Viton ® or silicone O-rings 

normally used to seal the Agilent 5890 Split and Splitless 

injection liners. Splitless liners have an internal diameter of 

6.5mm and split liners have an internal diameter of 6.35mm. 


Property Graphite Silicone PTFE Viton ® 

Max. Temp.: 450°C 230°C 230°C 200°C 

Chem. Rest.: Good Good Superb Superb 

Durability: Superb Moderate Superb Good 

Bleed: None Medium Low Low 

Color: Black Red White Black 

O-rings 

Size Qty. Part No. 

Graphite GC O-rings (450°C Limit) 

1/4" (6.5mm i.d.) 10 7160 

1/4" (6.35mm i.d.) 10 7176 

Silicone O-rings (230°C Limit) 

1/8" 100 7020 

3/16" 100 7021 

1/4" 100 7022 

PTFE O-rings (230°C Limit) 

1/8" 100 7023 

3/16" 100 7024 

1/4" 100 7025 

Viton ® O-rings (200°C Limit) 

1/8" 100 7017 

3/16" 100 7018 

1/4" 100 7019 

4639 

Injector Supplies for 

Agilent 5890/6890 GCs 

Inlet Seals for Agilent 5890/6890 GCs are available 

either stainless steel (similar to 

Agilent part no. 18740-20880) 

or gold plated (similar to Agilent 

part no. 18740-20885). Washers 

are included with the inlet seals. 

The Reducing Nut for Agilent 5890/6890 GCs is made from 

stainless steel and is similar to Agilent part no. 18740-20800. 

Injector Supplies for Agilent 5890/6890 GCs 


Inlet Seal, stainless steel (0.8mm hole) 1 15146 

10 151461 

25 151462 

Inlet Seal, gold plated (0.8mm hole) 1 15147 

10 151471 

25 151472 

Reducing Nut Replacement for Agilent 5890/6890 1 15145 

Washers, stainless steel (0.375" o.d.) 10 15148 

MSD Source Nut, brass 1 15104 

Accessories for 

Agilent and Varian ® 

Capillary Systems 

Agilent nuts are available in both standard and “deep well” 

versions, with either standard wrench flats or knurled for easy 

wrench-free fingertight connections. The deep well nuts have 

2.4mm deep ferrule wells to accommodate standard capillary 

ferrules, and the standard type has a 1.65mm deep ferrule 

well for Agilent-type capillary ferrules. 

6650 

6651 


Don't forget septa! 

See pages 289–292 for a complete selection of septa. 

Accessories for Agilent and Varian ® 


Capillary Nuts 

Capillary Nuts for Varian ® 2 154740 

Capillary Nuts for Agilent 2 154780 

Wrench-Free Capillary Nuts for Agilent 2 154785 

Deep Well Capillary Nuts for Agilent 2 154781 

Wrench-Free Deep Well for Agilent 2 154782 

Graphite Ferrules for Agilent 

Graphite Ferrules, 0.5mm Hole* 10 1547901 

Graphite Ferrules, 1.0mm Hole** 10 1548001 

Wrench 

5/16" x 1/4" Open-End Wrench ea 1998 

*For 0.25 and 0.32mm i.d. Capillary Column. 

**For 0.45 and 0.53mm i.d. Capillary Column. 



Septa Introduction 

How to Select a Septum for Your Application 

With so many septa to choose from, choosing a septum for 

your application can be a confusing task. There are a number 

of criteria you need to consider when selecting a septum. 

Make and Model of Gas Chromatograph 

This will determine what size of septum you need. 

Grace carries a full range of septum sizes to fit most gas 

chromatographs. 

Performance 

A high-performance, low-bleed septum is desirable so that 

septa bleed does not interfere with the chromatography 

of your sample. Keep in mind that all septa bleed to some 

extent. The amount of bleed visible to the detector is affected 

by several factors which include: 

• The composition and pretreatment of the septum material 

• The sensitivity setting of the detector amplifier 

• The type of detector 

• The injection port setting 

• Isothermal or temperature programming 

• The type of injection (direct, splitless, split, etc.) 

Choose a high-performance septum such as ThermoRed 

or XLB septa to minimize septa bleed. 

Injection Port Temperature 

The temperature of the septum never reaches the injection 

port temperature setting. The center of the injector heated 

zone, where the sample is deposited, is regulated to 

correspond to the injector port setting. Be sure to select a 

septum with a maximum temperature that exceeds your 

injection port temperature. 

Penetration/Resealing Qualities 

Ideally, you want a septum that is soft and easily penetrated 

by a syringe needle and has excellent resealing properties. 

This will prevent you from bending syringe needles and 

coring of the septum to give you longer septum life. Excellent 

resealing properties will prevent leaks that might adversely 

affect your chromatography or damage your analytical 

column. 





Septa Specifications 

Actual Size Drawing Measurement Chromatograph 

1/4" 

(6mm) 

3/8" 

(9.5mm) 

7/16" 

(11mm) 

1/2" 

(12.5mm) 

21/32" 

(17mm) 

*Use cylindrical plug septa, see page 291. 

Shimadzu*, SRI* 

Antek, Finnigan 9100, 9600, Gow-Mac, 

Agilent 5700, 5880 & 5900, IBM, 

Packard 427-433, Pye-Unicam (early 

models), Varian (except capillary inj.), 

Tracor 550, 560 

Carle, Agilent 5890,6850,6890. P-E 

Sigma, 900, 990, 3920, 8300, 8400, 

8500 Autosys, Varian (capillary injector), 

SPI, Varian 1078/1079 

Fisons/Carlo Erba, Finnigan 9500, 

Agilent 5750 and earlier models, 

Packard 409-421, Tracor 220, 222, 540 

Fisons/Carlo Erba 8000 Series, CE 

TRACE GC 

Septa Selection Guide 

Product Max. Temp. Color Durometer* Low Bleed 

ThermoRed 300°C Red 50–55 Excellent 

XLB 350°C Green 50–55 Excellent 

HT-X High 

350°C Blue 50–60 Excellent 

Performance 

General Purpose Blue 250°C Blue 40–45 Good 

Cylindrical Half-Hole 250°C Blue 50–55 Very Good 

Cylindrical Three-Layer 200°C Red 50–55 Excellent 

Three-Layer 300°C Red/ 50–55 Good 

White 

Four-Layer 225°C Red/ 50–55 Good 

White 

Microsep ® 

250°C Off-White 25–35 Good 

PTFE-lined 

Microsep ® Low-Bleed 250°C Yellow/ 25–35 Very Good 

PTFE-lined 

Off-White 

High-Temperature 350°C Red 50 Excellent 

Ultra Low-Bleed 

*Durometer—Hardness of the silicone material. The lower the number, the softer 

the material. 

4610 

gc accessories | septa 



Septa 

3- and 4-Layer Septa 

• Excellent sealing characteristics 

• Maximum temperature of 300ºC (3-layer) and 

225ºC (4-layer) 

The 3-Layer Septa consists of a layer of red silicone rubber, 

a layer of translucent soft silicone rubber and another layer 

of red silicone rubber. The 4-Layer Septa is the same but with 

a 10-mil (0.010") PTFE facing. 

Microsep ® PTFE-Lined Septa 

• Maximum temperature of 250ºC 

• Off-white silicone rubber with an ivory PTFE backing 

4593 


3-Layer Septa 

4595 4594 

4-Layer Septa 

3- and 4-Layer Septa 

Size (o.d.) Qty. Part No. 

3-Layer Septa 

3/8" (9.5mm) 100 7836 

7/16" (11mm) 100 7838 

1/2" (12.5mm) 100 7840 

4-Layer Septa 

3/8" (9.5mm) 100 7844 

7/16" (11mm) 100 7846 

1/2" (12.5mm) 100 7848 

High-Temperature, Ultra Low-Bleed Septa 

• Long life, minimum of 450 injections 


• Made of 100% premium silicone 

• Guide hole in center 

• Precision molded 

new 

Microsep ® PTFE-Lined Septa 


0.250" (6mm) 12 75801 

0.250" (6mm) 100 79391 

0.281" (7mm) 12 75810 

0.355" (9mm) 12 75803 

0.375" (9.5mm) 12 75804 

0.375" (9.5mm) 100 79411 

0.394" (10mm) 12 75805 

0.394" (10mm) 100 79451 

0.438" (11mm) 12 75807 

0.438" (11mm) 100 79471 

0.469" (12mm) 12 75813 

0.500" (12.5mm) 12 75806 

0.500" (12.5mm) 100 79491 

Microsep ® Low-Bleed PTFE-Lined Septa 


• Off-white silicone septa with yellow PTFE backing 

7434 

4591 

High-Temperature, Ultra Low-Bleed Septa 


3/8" (9.5mm) 50 8619148 

0.394" (10mm) 50 8619149 

7/16" (11mm) 50 8619160 

Shimadzu ® Plug 50 8619144 

Microsep ® Low-Bleed PTFE-Lined Septa 


3/8" (9.5mm) 50 7802 

3/8" (9.5mm) 2 x 50 78001 

3/8" (9.5mm) 20 x 50 78002 

7/16" (11mm) 50 7803 

7/16" (11mm) 2 x 50 78011 

7/16" (11mm) 20 x 50 78012 

1/2" (12.5mm) 50 7806 

1/2" (12.5mm) 2 x 50 78041 

1/2" (12.5mm) 20 x 50 78042 

290 



Septa 

General Purpose Blue Septa 

• Maximum temperature of 250°C 

(300°C for short periods) 

• Lifetime of up to 150 injections 

• Suitable for most GC analysis 

General Purpose Blue Septa 


1/4" (6mm) 100 6512 

1/4" (6mm) 10 x 100 65122 

3/8" (9.5mm) 100 6514 

3/8" (9.5mm) 10 x 100 65142 

7/16" (11mm) 100 6518 

7/16" (11mm) 10 x 100 65182 

1/2" (12.5mm) 100 6520 

1/2" (12.5mm) 10 x 100 65202 

12" x 12" Sheet ea 6528 

4603 

XLB Septa 

• Maximum temperature to 350°C 

• Extremely low bleed 

• Superior resealing properties 

• Soft, easily penetrated by 

syringe needle 

XLB Septa 


1/4" (6mm) 50 15427 

3/8" (9.5mm) 50 15428 

7/16" (11mm) 50 15429 

1/2" (12.5mm) 50 15430 

21/32" (17mm) 50 15039 

4607 

ThermoRed Septa 


• Packed in glass jars under strict 

QC standards to protect from 

contaminants 

ThermoRed Septa 


11/32'' (9mm) 25 15129 

3/8'' (9.5mm) 25 15131 

7/16'' (11mm) 25 15132 

1/2" (12.5mm) 25 15141 

21/32'' (17mm) 25 15133 

Plug Type (For Shimadzu ® and SRI) 25 15134 

General Purpose White Septa 


• Lifetime of over 100 injections 

• Designed for use in 90% of all 

GC analysis 

General Purpose White Septa 


3/8'' (9.5mm) 50 7812 

3/8'' (9.5mm) 2 x 50 78101 

7/16'' (11mm) 50 7813 

7/16'' (11mm) 2 x 50 78111 

1/2" (12.5mm) 50 7816 

1/2" (12.5mm) 2 x 50 78141 

4608 

4605 

HT-X High-Performance Septa 


• Long injection life—over 

200 injections 

HT-X High-Performance Septa 


1/4" (6mm) 50 15490 

5/16" (8mm) 50 15491 

3/8" (9.5mm) 50 15492 

7/16" (11mm) 50 15493 

21/32" (17mm) 50 154941 

1/2" (12.5mm) 50 15494 

12" x 12" Sheet ea 15496 

Plug Type (For Shimadzu ® and SRI) 50 15498 

Cylindrical Septa 

• 8mm long x 6mm o.d. with 

half-hole 

The standard half-hole septa 

is red silicone with an upper 

temperature limit of 225°C. 

Half-Hole Septa Shown Here 

3-layer septa features the 

shielding and reinforcing properties of PTFE. The plug septa 

are low bleed, have an upper temperature limit of 350°C, and 

are compatible with Shimadzu ® and SRI. 

4604 

4597 


Standard Blue Septa 


• Lifetime of over 50 injections 

Standard Blue Septa 


3/8'' (9.5mm) 100 7824 

7/16'' (11mm) 100 7826 

1/2" (12.5mm) 100 7830 

4601 

Cylindrical Septa 


Cylindrical Half-Hole Septa 

1/4" (6mm) 25 6524 

1/4" (6mm) 4 x 25 6526 

3-Layer 1/4" (6mm) 50 9602 

Cylindrical Plug Septa for Shimadzu ® and SRI 50 15498 




Merlin Microseal ® Septum 

• Lasts for thousands of 

injections 

• No instrument 

modification needed 

• Installation as easy as 

changing a standard 

septum 

• High-pressure model 

allows operation up to 

100psig 

The Merlin Microseal ® septum is a long-life replacement for 

the septum and septum nut on the capillary inlet system of 

an Agilent GC. It incorporates two sequential seals to provide 

a much longer period of use between seal changes. Use the 

Merlin Microseal ® septum with the 7673 Autosampler to allow 

many more samples to be run unattended while reducing the 

risk of lost or compromised data caused by septum leaks or 

fragmentation. It also allows for easier manual injections. 

The Microseal ® septum lasts longest when a syringe needle 

with a truncated cone shaped tip is used. It may also be used 

with the standard 0.63mm diameter (0.026", 23-gauge) needle 

recommended for use with the 7673 Autosampler. 

Merlin Microseal ® Septum* 

Description 

Part No. 

Microseal ® Septum Kits 

For Agilent GCs 

Microseal ® Nut and 2 Septa 80060 

Microseal ® Nut and 1 Septum 80062 

Microseal ® High-Pressure Septum Kits 

Standard Kit, Nut and 2 Septa 80190 

Starter Kit, Nut and 1 Septum 80192 

For Varian ® 1078 and 1079 GCs with SPME Injector 

Varian ® Kit, Nut with Start Switch, 1 Septum and O-ring 80031 


For Agilent GCs 

Microseal ® Septum, ea 80063 

Microseal ® High-Pressure Septum, ea 80194 

Microseal ® High-Pressure Nut, ea 80196 

For Varian ® 1078 and 1079 GCs with SPME Injector 

Microseal ® High-Pressure Septum, ea 80035 

*Regular Merlin Microseal ® has a 30psig limit. High-Pressure Merlin Microseal ® has a 

100psig limit. 

5070 

Septa Accessories 

Septa-Out 

Safely extract cylindrical septa without 

the danger of breaking your glass 

column or sampling bulb port. 

Septa-Out 

Description 

Part No. 

Septa-Out 6412 

Heat-Resistant Gloves 

• Protect your hands from burns 

• Heavyweight cotton/Kevlar ® 

combination 

Knit construction offers superior comfort 

and lessens hand fatigue. Gloves are 

ambidextrous and feature a glove-withina-glove 

design. 

Heat-Resistant Gloves 


Heat-Resistant Gloves pr 2906 

Inlet Pressure Gauge 

Insert the needle directly into the inlet of any gas 

chromatograph for direct pressure measurement. 

The small, 0.028" o.d. needle permits repeated 

inlet pressure checks without damage to the 

septum. 

Inlet Pressure Gauge 

Description 

Part No. 

0–30psig 86805 

0–60psig 86806 

0–100psig 86807 

0–200psig 86808 

Replacement Needle Assembly 18260 

4585 

4582 

4588 

Septum Remover 

90º hooked point allows for safe septum removal. Aluminum 

body. 8" length. 

Septum Remover 

Description 

Part No. 

Septum Remover 24030 

4584 

Septum Needle Guide 

This septum needle guide helps reduce 

bent syringe needles and extends 

septum life. With the septum needle 

guide you get double leak protection. 

A cylindrical septum acts as a double seal should your 

primary septum start leaking. Needle guide is self-centering 

to aid in on-column injection. 

Septum Needle Guide 

Description 

Part No. 

Needle Guide, 7/16" x 20 Thread* 6401 

Needle Guide, 7/16" x 24 Thread 6403 

Needle Guide without Nut 6400 

Drill Bit for Enlarging Hole 6402 

Replacement Cylindrical Septa, 25/pk 6524 

*Fits most injection ports including: PE-3920 and Sigma Series; HP5700, 5800, and 

6900 Series (5890 and 6890). 

4583 

292 



Flow Control Valves 

Parker Toggle Valves 

These forged-body valves are positive, soft-seat 

shut-off valves designed for quick opening and 

closing. Shut-off force is constant because of 

the spring load. The handle locks in the open Part No. 2328 

or closed position. A TFE stem tip provides a 

leak-tight seal. The maximum working pressure is 250psig; 

maximum temperature is 93°C. They are made of brass or 

316 stainless steel with TFE seat, fluoroelastomer polymer 

packing and nylon handle. All other parts are 316 stainless 

steel. 

Parker Toggle Valves 

Description 

Part No. 

1/8", Brass 2328 

1/4", Brass 2332 

1/8", Stainless Steel 2336 


Nupro ® and Whitey ® Plug and Ball Valves 

The plug valves are constructed of brass or stainless steel 

and TFE. The compact plug valves can be used up to 

3000psig and the temperature limit is 200°C. They have 

a plug orifice of 0.172" for high flow capacity. 

best 

seller 

The 2-way and 3-way ball valves can be used up to 65°C and 

are pressure-rated to 2500psig. Both can be panel-mounted 

in a 19/32" hole. The 1/8" valves have a 0.093" orifice while 

the 1/4" valves have a 0.125" orifice. 

5232 5233 5234 

Part No. 14320 Part No. 14318 Part No. 14316 

Nupro ® and Whitey ® Plug and Ball Valves 

Plug Valve 

Part No. 

2-Way Ball Valve 

Part No. 

3-Way Ball Valve 

Part No. 

Description 

1/8", Brass 14320 14318 14316 

1/4", Brass 14319 14317 14315 

1/8", Stainless Steel 16013 16017 16022 


Nupro ® and Whitey ® Fine Metering Valves 

These valves give extremely fine control in the 0–400cc/min 

gas flow range. The maximum pressure rating is 1000psig 

at 70°C. The maximum temperature limit for brass valves is 

150°C; 200°C for stainless steel. They can be panel mounted 

in a 37/64" hole. 

Nupro ® and Whitey ® Fine Metering Valves 

Description 

Straight Pattern 

Part No. 

Angle Pattern 

Part No. 

1/8", Brass 8600 8608 

1/4", Brass 8602 8610 

1/8", Stainless Steel 8604 8612 


5225 

Hoke ® Toggle Valves 

These valves feature a simple, reliable, 

low-maintenance design that is well suited for 

a wide variety of applications. They are made 

of brass or stainless steel and include longlasting 

fluoroelastomer polymer seat and o-ring Part No. 12131 

5259 

packing for positive pressure or vacuum applications. 

The maximum operating pressure is 200psig and the 

maximum operating temperature is 149°C. 

Hoke ® Toggle Valves 

Description 

Part No. 

Straight Toggle Valves 

1/8", Brass 12131 

1/4", Brass 12133 



Hoke ® Plug and Ball Valves 

The plug valves are constructed of brass or stainless steel 

and PTFE. They have an upper pressure limit of 3000psig 

and an upper temperature limit of 205°C. They have a plug 

orifice of 0.093". 

The 2-way ball valves are made of brass or stainless steel 

and PTFE. They have an upper temperature limit of 179°C 

and an upper pressure limit of 1500psig. The 1/8" version has 

a 0.093" orifice while the 1/4" version has a 0.187". 

The 3-way ball valves are made of brass or stainless steel 

and PTFE/Nylatron ® . They have an upper pressure limit of 

500psig and an upper temperature limit of 179°C. Both sizes 

have a 0.187" orifice. 

5262 5260 5263 

Part No. 12126 Part No. 12119 Part No. 12123 

Hoke ® Plug and Ball Valves 

Plug Valve 2-Way Ball Valve 3-Way Ball Valve 

Description 

Part No. 

Part No. 

Part No. 

1/8", Brass 12126 12118 12122 

1/4", Brass 12128 12120 12124 



Plug Valve Repair Kit — — 12130 

Hoke ® Fine Metering Valves with Vernier Knob 

These valves allow reproducibility of precise flows. All valves 

use a TFE seat seal and packing. Brass valves are rated at 

3000psig; stainless steel at 5000psig. Temperatures up to 

230°C can be used but at lower pressures. 

Hoke ® Fine Metering Valves 

Straight Pattern 

Part No. 

Angle Pattern 

Part No. 

Description 

1/8", Brass 86001 86081 

1/4", Brass 86021 86101 



gc accessories | valves 



Valco ® Sampling/ 

Switching Valves 

Zero-Volume Fittings Allow for Direct . 

Valve Connections 

• Easily adapt to any type of tubing 

• Eliminate pressure surges during sample injections 

• Carefully leak tested 

Accessories for Valco ® 

Sampling/Switching Valves 


Valco ® Stainless Steel Sample Loops* 

Loop Size 

Use with 1/16" Valves 

Part No. 


Part No. 

15µL 91056 91106 

25µL 91057 91107 

50µL 91058 91108 

100µL 91059 91109 

250µL 91050 91100 

500µL 91051 91101 

1.0mL 91052 91102 

2.0mL 91053 91103 

5.0mL 91054 91104 

10.0mL 91055 91105 

*One pair of nuts and ferrules are included. 

gc accessories | valves 

What’s included: zero-volume fittings, mounting 

bracket, and handle. 

Valco ® Sampling/Switching Valve Specifications 

Valve Body: Type 316 Stainless Steel 

Valve Seal: 

Carbon-filled PTFE (Medium-Temp.) or 

Carbon-filled Polyimide (High-Temp.) 

Outlet Fitting: 1/16" zero-volume fittings or 

1/8" zero-volume fittings (included) 


Max. Temp: 

175°C for medium-temperature valves 

300°C for high-temperature valves* 

Active Ports: 3, 4, 6, 8, or 10 Ports (Note: a 10-port valve can 

be used as a 6- or 8-port valve) 

Mounting: 

Valves supplied with angle mounting brackets for 

mounting on the side of the instruments or they 

can be flush mounted. Valves are tapped with 

8-32TPI holes. Most GCs are designed to accept 

these valves. 

*Cannot be used below 200°C. 

4678 

Zero-Volume Fittings 

Column 

Inlet 

Zero-Volume Fittings 

Valve Body 


Part No. 

Sample 

Loop 


Part No. 

Description 

Nuts, 5/pk 458015 458025 

Ferrules, 5/pk 458125 458155 

1577 

Valco ® Sampling/Switching Valves* 

Diagram Ports Fittings 

Medium-Temp. 

Valve Part No. 

High-Temp. 

Valve Part No. 

0345 

3-Port 1/16" Zero-Volume 9099 39099 

1/8" Zero-Volume 9101 39101 

0346 

2102 

2103 


1/8" Zero-Volume 9104 39104 


1/8" Zero-Volume 9110 39110 


1/8" Zero-Volume 9116 39116 


2104 1/8" Zero-Volume 9120 39120 

*By special order, valves can be fabricated from Hastalloy C. Call for pricing information. 





more info 

For more detailed list of Valco ® Accessories, visit our website at 

www.discoverysciences.com. 

294 


General 

Chromatography 

SPE . . . . . . . . . . . . . . . . . . . . . . . 296–316 

SPE Introduction . . . . . . . . . . . . . . . . . . . 296–299 

Alltech ® SPE Sorbent Introduction . . . . . . . . . . 300–301 

Alltech ® Extract-Clean and Ultra-Clean Columns. 302–306 

Reversed-Phase . . . . . . . . . . . . . . . . . . . . . . . 302 

Normal-Phase . . . . . . . . . . . . . . . . . . . . . . . . . 303 

Ion-Exchange . . . . . . . . . . . . . . . . . . . . . . . . . 304 

Specialty . . . . . . . . . . . . . . . . . . . . . . . . . 305–306 

Alltech ® Maxi-Clean Cartridges . . . . . . . . . . . .307–310 

Reversed-Phase . . . . . . . . . . . . . . . . . . . . . . . 308 

Normal-Phase . . . . . . . . . . . . . . . . . . . . . . . . . 309 

Ion-Exchange . . . . . . . . . . . . . . . . . . . . . . . . . 310 

Alltech ® 96-Well Plates. . . . . . . . . . . . . . . . . . . 311 

Vydac ® SPE Columns . . . . . . . . . . . . . . . . . . . 311 

GracePure SPE Sorbents and Columns . . . . . . 312–313 

Vacuum Manifolds . . . . . . . . . . . . . . . . . . 314–315 

SPE Accessories . . . . . . . . . . . . . . . . . . . . . 316 

Filtration . . . . . . . . . . . . . . . . . . . .317–322 

Filtration Introduction. . . . . . . . . . . . . . . . . 317–318 

Syringe Filters . . . . . . . . . . . . . . . . . . . . 319–320 

Other Filters and Accessories . . . . . . . . . . . . 321–322 

Standards and Reagents . . . . . . . . . . .323–335 

Derivatization Reagents . . . . . . . . . . . . . . . 323–327 

Ion-Pair Reagents . . . . . . . . . . . . . . . . . . . . . 328 

Gas Standards . . . . . . . . . . . . . . . . . . . . 329–331 

FAME & Hydrocarbon Standards . . . . . . . . . . 332–333 

IC Standards and Buffers . . . . . . . . . . . . . . 334–335 

Syringes . . . . . . . . . . . . . . . . . . . .336–347 

Introduction. . . . . . . . . . . . . . . . . . . . . . 336–336 

Hamilton ® Syringes. . . . . . . . . . . . . . . . . . 337–342 

VICI ® Precision Syringes. . . . . . . . . . . . . . . . . 343 

SGE ® Syringes . . . . . . . . . . . . . . . . . . . . 344–346 

Popper Syringes and Syringe Accessories . . . . . . . . 347 

Vials. . . . . . . . . . . . . . . . . . . . . . . 348–383 

Introduction. . . . . . . . . . . . . . . . . . . . . . 348–353 

8 x 40mm Vials. . . . . . . . . . . . . . . . . . . . . . 354 

12 x 32mm Vials . . . . . . . . . . . . . . . . . . . 355–361 

15 x 45mm Vials . . . . . . . . . . . . . . . . . . . 362–363 

Headspace and Specialty Vials . . . . . . . . . . . 364–367 

Storage Vials, Shell Vials, and Closures. . . . . . . 368–375 

EPA and I-CHEM Vials . . . . . . . . . . . . . . . 376–378 

96-Well Products . . . . . . . . . . . . . . . . . . . . . 379 

Vial Accessories . . . . . . . . . . . . . . . . . . . 380–383 

Tubing . . . . . . . . . . . . . . . . . . . .384–391 

Organizers and Tools . . . . . . . . . . . . .392–393

general chromatography 

solid phase extraction 

SPE Introduction 

How to Choose an SPE Product 

1. Characterize the Sample 

Factors such as the analyte’s polarity relative to the 

matrix, the presence of charged functional groups, 

solubility, molecular weight, etc., determine how strong 

the analyte is retained by the packed bed. 

2. Select a Retention Strategy 

There are two basic methods for sample treatment: 

a. Select the packing bed to retain the desired analyte. 

The contaminants are washed off and the desired 

analyte is then eluted for analysis. 

b. Select the packing bed to retain the contaminants 

and the desired analyte passes directly through. 

3. Select the Proper Packing Type 

Select the proper packing type for the cleanest extract 

with the highest recovery. 

a. Reversed-phase packings are hydrophobic, silica-based 

materials that retain moderately polar to non-polar 

compounds from a polar matrix while washing off polar 

interferences. Or you can retain non-polar contaminants 

while the polar compounds pass through unretained. 

b. Normal-phase packings are hydrophilic, silica-based 

materials that retain polar compounds from a nonpolar 

matrix while washing off non-polar interferences. 

Or you can retain polar contaminants while non-polar 

compounds pass through unretained. 

c. Ion-exchange resins retain charged compounds or 

remove ionic interferences. 

4. Optimize Conditions for Best Results 

Select proper bed size and suitable conditioning, wash 

and elution solvents. 

a. Poor sample recovery often occurs when the packed 

bed dimensions are not optimized. An excessive bed 

weight results in incomplete elution while an insufficient 

bed weight results in incomplete retention. 

b. Consider the solvent strength relative to the packing 

material. The final conditioning solvent should be 

weak so it doesn’t act as an eluting solvent. Buffers 

should be used to control ionization of potentially 

charged compounds. 

c. Wash solvents should remove weakly retained 

interferences without being strong enough to elute 

the analyte. 

d. Elution solvents should be strong enough to completely 

elute an analyte in a small volume (1–2mL). 

Solid-Phase Processing Methods 

By Syringe 

Process individual 

samples via luer hub 

syringe. Connect 

syringe directly to SPE 

cartridges or to SPE 

columns via adapters 

found on page 316. 

By Vacuum 

Manifold 

Process multiple 

samples on a vacuum 

manifold using 

SPE columns. Extend 

volume via reservoirs. 

Products found on 

pages 314–316. 

By Filter Flask 

Process large volume 

samples by placing 

SPE tube with an 

online filtration system. 

See page 130. 

For Environmental Applications 

Recommended SW-846 Cleanup Methods 

Analyte Group 

EPA . 

Method No. 

Recommended . 

SPE Sorbents 

Aniline and Derivatives 3620 C18 

Chlorinated Pesticides 3620 C18, Florisil ® 

Chlorinated Hydrocarbons 3620 C18, Phenyl 

Haloethers 3620 C18, Florisil ® 

Nitroaromatics and Cyclic 

3620 DVB, IC-RP 

Ketones 

Nitrosamines 3610, 3620 C18 

Organochlorine Pesticides 3620 C18, Florisil ® 

Organophosphorus Pesticides 3620 Florisil ® , Carbograph 

PCB’s 3620, 3630 C18, SCX, Carbograph 

Petroleum Waste 3611 Alumina 

Phenols 3630 C18, Phenyl 

Phthalate Esters 3610, 3620 C18, DVB 

Polynuclear Aromatic 

Hydrocarbons 

3630 C18, Phenyl 

4915 

6275 

5953 

296 




SPE Method Development 

SPE method development typically contains four steps: 

Step 1: Condition 

The conditioning step is composed of two substeps; the first activates 

the sorbent ligands, the second equilibrates the sorbent bed. 

Step 2: Load 

In the load step, sample is applied to the SPE device. Matrix and flow rate 

are optimized to quantitatively retain target analytes. 

Step 3: Wash 

In the wash step, choose a solvent that elutes impurities but retains target 

analytes. Often the second conditioning solvent is a suitable wash solvent. 

Step 4: Elute 

The elution step ideally removes all target analytes with minimal solvent 

to maximize sensitivity. Sometimes this requires a combination of solvents 

to break both the primary and secondary interactions. 

7055 

Reversed-Phase Extraction Procedure 

Mechanism: Bind moderately polar to non-polar 

compounds from a polar sample matrix 

Normal-Phase Extraction Procedure 

Mechanism: Bind polar compounds from 

a non-polar sample matrix 

Ion-Exchange Extraction Procedure 

Mechanism: Bind charged (negative/anionic 

or positive/cationic) compounds 

General Method Development Procedures 

Step 1—Condition Step 2—Load Step 3—Wash Step 4—Elute 

Methanol followed Process sample at a Water or water: Methanol or acetonitrile. May need to 

by water 

flow rate of 1–5mL/min methanol (95:5) add strong acid or base to organic solvent 

to break secondary interactions. 

IPA followed 

by hexane 

Methanol:water 

(50:50) followed by 

low ionic strength 

(0.1M) buffer 

Process sample at a 

flow rate of 1–5mL/min 

Apply slowly: less than 

or equal to 1mL/min ion 

exchange kinetics are 

slower than reversedor 

normal-phase 

Hexane or 

hexane:IPA 

(98:2) 

Methanol:low 

ionic strength 

(0.1M) buffer 

(10:90) 

IPA, ethyl acetate, acetone, or hexane: 

IPA (50:50) 

High ionic strength (0.5M–1.0M) buffer 

or modify pH such that the analyte is 

uncharged. May need to add organic 

to break hydrophobic interactions. 

Recommended Usage Guidelines* 

Bed Size: 50mg 100mg 200mg 500mg 500mg 1000mg 2000mg 5000mg 10,000mg 

Sorbent Retention Capacity: 2.5mg 5mg 10mg 25mg 25mg 50mg 100mg 250mg 500mg 

Condition Volume (4-bed volumes) 0.30mL 0.60mL 1.20mL 3.00mL 3.00mL 6.00mL 12.00mL 30.00mL 60.00mL 

Wash Volume (6-bed volumes) 0.45mL 0.90mL 1.80mL 4.50mL 4.50mL 9.00mL 18.00mL 45.00mL 90.00mL 

Minimum Elution Volume (3-bed volumes) 0.23mL 0.45mL 0.90mL 2.25mL 2.25mL 4.50mL 9.00mL 22.50mL 45.00mL 

*Estimates only. Must optimize for each application. 

tech tip 

To calculate sorbent bed volume, use 150µL for every 100mg 

of sorbent. 

tech tip 

Retention capacity describes the total amount that an SPE 

sorbent will bind. This includes all compounds retained—analytes 

of interest as well as the contaminants. 

tech tip 

Minimum elution volume recommended in bed size chart above 

will offer best sensitivity, but more solvent may be required 

depending on application. 

Solvent Polarity Chart 

Less Polar (Stronger Reversed-Phase Elution) 

Hexane 

Isooctane 

Cyclohexane 

Carbon tetrachloride 

Toluene 

Chloroform 

Methylene chloride 

Tetrahydrofuran 

Ethyl ether 

Acetone 

Acetonitrile 

Pyridine 

Isopropanol 

Ethanol 

Methanol 

Water 

Acetic acid 


More Polar (Stronger Normal-Phase Elution) 




Grace Davison ® has been making Davisil ® silica for over a quarter century. It is the 

foundation for all our silica-based SPE products, including Alltech ® , Vydac ® , and 

GracePure brands. Having complete control over the entire media process ensures 

highly consistent performance, and uninterrupted supply. 

Experts in Media Production 

Using a consistent and pure silica base, and employing tightly controlled bonding 

techniques, insures predictable analyte-sorbent interactions. Both of these aspects 

also play equal importance in manufacturing a bonded phase with high and 

reproducible recoveries. 

Highest Quality Control 

Every part of our manufacturing process is carefully monitored. From silica production 

to final product, we perform multiple quality tests, and provide a comprehensive 

quality assurance certificate. 

7377 

Grace SPE Product Lines 

Alltech ® Extract-Clean Columns 

Alltech ® Ultra-Clean Columns 


Format: SPE Columns 

Sizes: 1.5, 4, 8, 15, 25, 75mL (the entire tube volume) 

Summary: In production for over 25 years, with proven 

consistency, this is our most comprehensive SPE 

product line. It includes 30 media types in over 10 

different bed weights. And with a complete offering 

of reversed, normal, and specialty medias exhibiting 

unique retention properties, you are sure to find the 

packing that delivers a cleaner, more concentrated 

sample. See pages 302–306. 

Alltech ® Maxi-Clean Cartridges 

Format: SPE Cartridges 

Sizes: 300, 600, 900mg (media amount, not device 

volume) 

Summary: The Maxi-Clean line is offered in many 

of the same media as the Extract-Clean line, but 

slightly paired down, with over 20 chemistries available. 

This lure hub cartridge device is not as prevalent in the 

SPE industry, and while manual processing is most 

common, this format offers a number of other interesting 

processing options, including multimedia extractions. 

See page 307–310. 

Vydac ® Columns 


Sizes: 1, 3mL (volume above the packing) 

Summary: Ideal for extraction, concentration and 

cleanup of biological samples. This 300Å silica-based 

media has the same properties as the industry-leading 

Vydac ® TP HPLC packing. Offered in C18 and C4, use 

for a variety of protein and peptide applications. See 

page 311 for details. 


Sizes: 4, 8mL (the entire tube volume) 

Summary: Choose this ultra-low extractable version 

for very sensitive applications. Nine selected media are 

packed into highly inert fluorinated polypropylene tubes 

with PTFE frits. Less expensive than glass extraction 

devices, this durable format offers comparable inertness 

without the added concern of being easily broken. 


Alltech ® 96-Well Plates 

Format: 96-Well Plates 

Size: 2mL (the individual well volume) 

Summary: Ideal for high-throughput SPE processing, 

this 8" x 12" standard format fits into traditional 96-well 

automated equipment. We offer six different medias 

in three bed weights packed in 2mL square wells. See 

page 311. Empty plates are also available on page 316. 

GracePure Columns 


Sizes: 1, 3, 6, 12, 20, 60mL (volume above the packing) 

Summary: Our in-house capability to make everything 

from the silica particle to the finished good means we 

can deliver GracePure as the best value in SPE. With 

a concise offering of 11 sorbents in six bed weights, this 

high-quality SPE product line is a result of operational 

excellence that Grace Davison ® is known for. See 

pages 312–313. 

298 




Device Options 

Device Specifications 

Device Housing Frit Material 

Extract-Clean Columns Polypropylene 20µm Polyethylene 

Ultra-Clean Columns Treated 10µm PTFE 

Polypropylene 

GracePure Columns Polypropylene 20µm Polyethylene 

Vydac ® Columns Polypropylene Glass Fiber Filter Paper with 

Polyethylene Mesh Support 

Maxi-Clean Cartridges Polypropylene 20µm Polyethylene 

96-Well SPE Plates Polypropylene 20µm Polyethylene 

SPE Columns 

• Open top tubes with male luer bottom 

• Process multiple samples with vacuum manifold or automated SPE instruments 

• Process individual samples manually with use of adapter and syringe 

Traditionally, differing nomenclature has been used to 

describe SPE column size. Sometimes columns are 

described in terms of full volume. Alternatively, the volume 

above the bed weight may also be used. Below is a crossreference 

for your convenience. 

Alltech ® GracePure/Vydac ® 

1.5mL 

1mL 

4mL 

3mL 

8mL 

6mL 

15mL 

12mL 

25mL 

20mL 

75mL 

60mL 

25mL 

7309 

15mL 

7310 

8.0mL 

7311 

4.0mL 

7312 

1.5mL 

SPE Cartridges 

• Female luer top and male luer bottom 

• Process single cartridge by syringe 

or multiple cartridges by vacuum 

• Stack for multi-step extractions 

7313 


SPE 96-Well Plates 

• High throughput SPE devices 

• 8" x 12" standard 96-well format 

• 2mL square wells 

7323 

7324 

300mg Bed 

600mg Bed 

7308 

75mL 

4888 

7322 

900mg Bed 



Alltech ® SPE Sorbents 

Alltech ® SPE Sorbents are packed into three device types; 

columns, cartridges, and 96-well plates. See chart for availability. 

Extract-Clean 

SPE Columns 

Ultra-Clean 

Ultra-low Extractable 

SPE Columns 

Maxi-Clean 

SPE Cartridges 

96-Well Plates 

High Throughput 

SPE Processing 

pages 302-306 pages 302-305 pages 307-310 page 311 

Reversed-Phases (Non-Polar) Sorbent Specifications 


%. 

Carbon 

End-. 

capped 

Average 

Particle 

Size 

Pore 

Size Features Benefits 

Packing 

Base 

Prevail C18 Silica 11.0% Yes 50µm 60Å 100% water wettable Hydrophilic/hydrophobic retention. 

Phase remains active even 

when completely dry. Can omit 

preconditioning step. 

x x x 

Standard C18 Silica 6.0% Yes 50µm 60Å Low carbon load C18 General purpose phase. x x x x 

High-Flow C18 Silica 8.0% Yes 100µm 60Å Large particle Less flow resistance for faster flow x 

rates of large volume sample. 

High-Capacity C18 Silica 17.0% Yes 50µm 60Å High carbon load Maximum capacity phase. x x 

Large Pore C18 Silica 14.0% Yes 50µm 150Å Larger than average pore Ideal for compounds >1500MW. x x 

size 

Octyl (C8) Silica 4.5% Yes 50µm 60Å Less hydrophobic than C18 Less retention of highly hydrophobic x x x 

compounds. Use when C18 is 

too retentive. 

Ethyl (C2) Silica 5.5% Yes 50µm 60Å Short chain functional group Less retention of highly hydrophobic x x 

is less hydrophobic than C8 compounds. Use when C8 is 

too retentive. 

Phenyl (PH) Silica 3.8% Yes 50µm 60Å Aromatic structure Highly selective for aromatic 

compounds. 

x x 

Normal-Phases (Polar) Sorbent Specifications 

Packing 

Base 

%. 

Carbon 

End-. 

capped 

Average 

Particle 

Size 

Pore 


Silica (SI) Silica — — 50µm 60Å Highly polar surface Most common polar phase. x x x x 

Extract-Clean 

Extract-Clean 

Ultra-Clean 

Ultra-Clean 

Maxi-Clean 

Maxi-Clean 

96-Well 

96-Well 

Aminopropyl (NH2) Silica 5.0% No 50µm 60Å Polar phase with slight anion 

exchange properties 

Ideal for carbohydrates or generally 

with analyses containing hydroxyl 

functional groups. 

Cyanopropyl (CN) Silica 6.0% Yes 50µm 60Å Unique selectivity Can be used in normal-phase or 

reversed-phase modes. 

Diol (2OH) Silica 4.0% No 50µm 60Å Polar surface with minor Wets easily and offers more 

hydrophobic retention reproducibility. 

Florisil ® (FL) 

Magnesium — — 75– 60Å Highly polar surface Referenced in many EPA methods. 

Silicate 

150µm 

Ideally suited for pesticides and 

metals. 

Florisil ® PR (FL-PR) Magnesium — — 75– 60Å Specifically tested for Ensures most inert batches suitable 

Silicate 

150µm chlorinated pesticides for highly active compounds. 

Alumina Acidic (AL-A) Aluminum — — 130µm 100Å Alumina washed with acid Increase capacity for acidic 

Oxide 

surface 

compounds. 

Alumina Basic (AL-B) Aluminum — — 130µm 100Å Alumina washed with base Increase capacity for basic 

Oxide 

surface 

compounds. 

Alumina Neutral (AL-N) 

Aluminum 

Oxide 

— — 130µm 100Å Alumina washed with neutral 

surface 

Interacts with highly aromatic 

compounds and neutral hydroxyls. 

x x 

x x x 

x x 

x x x 

x x x 

x x 

x x 

x x 

300 



Alltech ® Spe Sorbents 

Specialty Packings Specifications 

%. 

Carbon 

End-. 

capped 

Average 

Particle 

Size 

Pore 


Packing 

Base 

DVB 100% DVB — — 40µm — 100% DVB Greater capacity than C18 for 

general SPE. Also free vinyl surface 

groups make a suitable solid-phase 

synthesis support. 

Carbograph 

Graphitized 

Carbon 

— — 38– 

125µm 

Drug-Clean SB-C Silica — — 50µm 60Å Silica-based mixed mode 

Drug-Clean SB-A Silica — — 50µm 60Å Silica-based mixed mode 

Drug-Clean PB Polymer — — 30µm — Polymer-based mixed mode 

C8/cation exchange 

AFT 

C18, Silica, 

and Alumina 

6 — 50– 

130µm 

— Graphitized Carbon Retains polar organics in aqueous 

matrices. Ideally suited for acid, 

base-neutral extraction of pesticides 

and herbicides. 

x x 

Ideal for drugs of abuse. x x 

C8/cation exchange 

Ideal for drugs of abuse. x x 

C8/anion exchange 

— Unique blend of reversed 

and normal phases. 

pH stable with no conditioning 

required. Extract acidic, neutral 

and basic drugs of abuse from 

single column. 

Ideal for Aflatoxins. 

Extract-Clean 

x 

x 

x 

Ultra-Clean 

Maxi-Clean 

96-Well 

x 

General Ion-Exchange Sorbent Specifications 

Packing Base Counter Ion 

SCX Styrene- 

DVB 

SAX 

Styrene- 

DVB 

Particle 

Size 

Functional 

Group 

Hydrogen 50µm Benzene 

Sulfonic 

Acid 

Acetate 50µm Tetramethyl 

Ammonium 

Ion Chromatography Sorbent Specifications 


IC-OH Styrene- 

DVB 

IC-H 

IC-Ag 

IC-Ba 

IC-Na 

IC-Chelate 

Styrene- 

DVB 

Styrene- 

DVB 

Styrene- 

DVB 

Styrene- 

DVB 

Styrene- 

DVB 

Particle 

Size 

Molecular 

Exclusion 

Limit 

Hydroxide 50µm 1000 

Daltons 

Hydronium 50µm 1000 

Daltons 

Silver 50µm 1000 

Daltons 

Barium 50µm 1000 

Daltons 

Sodium 50µm 1000 

Daltons 


Daltons 

Exchange 

Capacity Retains Applications 

2.0meq/mL 

1.0meq/mL 

Cations, (+) charged 

compounds 

Anions, (–) charged 

compounds 

Exchange 


IC-RP Polystyrene — 550µm — — Hydrophobic 

components 

Remove/concentrate basic 

compounds. 

Remove/concentrate acidic 

compounds. 

1.0meq/mL Anions Exchanges anions for hydroxide. May be 

used to remove or concentrate anions 

from sample and to increase pH of acidic 

samples. Removes cations that form 

insoluble hydroxide salts. 

2.0meq/mL Cations Exchanges cations for H + . May be 

used to remove or concentrate cations 

from sample and to reduce pH of basic 

samples. 

2.0meq/mL Chloride Iodide Bromide Removes excess halides through 

formation of Ag-halide salts. 

2.0meq/mL Sulfate Removes excess sulfate through 

formation of BaSO. 

2.0meq/mL Cations Exchanges cations for Na + . May be used 

to remove or retain cations from sample 

without changing the pH of the sample. 

0.4meq/mL Polyvalent metal ions Exchanges transition metals and divalent 

cations for Na + . May be used to remove 

or retain divalent cations and transition 

metals from sample. 

Removes surfactants, organic acids, and 

other organic substances. Inorganic ions 

pass through. 

Extract-Clean 

Ultra-Clean 

Maxi-Clean 

96-Well 

x x x x 

x x x x 

Extract-Clean 

Ultra-Clean 

Maxi-Clean 

96-Well 

x x 

x x 

x x 

x x 

x x 

x x 

x x 




Alltech ® Extract-Clean and Ultra-Clean Columns 

Reversed-Phase Sorbents 

4886 

Extract-Clean Columns 

• Available: Prevail C18, Standard C18, 

High-Flow C18, High-Capacity C18, 

Octyl (C8), Ethyl (C2), Phenyl (PH) 

• General purpose SPE column with the most 

comprehensive sorbent offering 

Ultra-Clean Columns 

• Available: Standard C18, Octyl (C8) 

• Low extractable version for highly sensitive 

applications—fluorinated polypropylene 

columns with PTFE frits 


%. 

Carbon 

End-. 

capped 

Average 

Particle 

Size 

Pore 


Functional Group Base 

Prevail C18 Silica 11.0% Yes 50µm 60Å 100% water wettable Hydrophilic/hydrophobic retention. Phase 

remains active even when completely dry. 

Can omit preconditioning step. 

Standard C18 Silica 6.0% Yes 50µm 60Å Low carbon load C18 General purpose phase. 

High-Flow C18 Silica 8.0% Yes 100µm 60Å Large particle Less flow resistance for faster flow rates 

of large volume sample. 

High-Capacity C18 Silica 17.0% Yes 50µm 60Å High carbon load Maximum capacity phase. 

Large Pore C18 Silica 14.0% Yes 50µm 150Å Larger than average pore size Ideal for compounds >1500MW. 

Octyl (C8) Silica 4.5% Yes 50µm 60Å Less hydrophobic than C18 Less retention of highly hydrophobic 

compounds. Use when C18 is too 

retentive. 

Ethyl (C2) Silica 5.5% Yes 50µm 60Å Short chain functional group is less 

hydrophobic than C8 

Less retention of highly hydrophobic 


retentive. 

Phenyl (PH) Silica 3.8% Yes 50µm 60Å Aromatic structure Highly selective for aromatic compounds. 


Alltech ® Reversed-Phase Extract-Clean Columns 

Bed Weight Column Size Qty. Part No. 

Prevail C18 

100mg 1.5mL 100 605001 

130mg 4.0mL 50 605130 

500mg 4.0mL 50 605250 

500mg 8.0mL 30 605350 

1000mg 8.0mL 30 605430 

Standard C18 

50mg 1.5mL 100 204900 

100mg 1.5mL 100 205000 

200mg 4.0mL 50 205150 

500mg 4.0mL 50 205250 

500mg 8.0mL 30 205350 

1000mg 8.0mL 30 205430 

2000mg 8.0mL 30 205450 

2000mg 15mL 30 205462 

5000mg 25mL 20 225450 

10,000mg 75mL 16 235410 

High-Flow C18 

500mg 4.0mL 50 215250 

1000mg 8.0mL 30 215430 

High-Capacity C18 

50mg 1.5mL 100 255050 

100mg 1.5mL 100 255100 

200mg 4.0mL 50 255200 

500mg 4.0mL 50 255300 

500mg 8.0mL 30 255350 

1000mg 8.0mL 30 255430 

2000mg 15mL 30 255440 

5000mg 25mL 20 255450 

10,000mg 75mL 16 255460 

Alltech ® Reversed-Phase Extract-Clean Columns (continued) 


Octyl (C8) 

100mg 1.5mL 100 206000 

200mg 4.0mL 50 206150 

500mg 4.0mL 50 206250 

500mg 8.0mL 30 206350 

Ethyl (C2) 

100mg 1.5mL 100 207300 

200mg 4.0mL 50 207450 

500mg 4.0mL 50 207550 

10,000mg 75mL 16 207616 

Phenyl (PH) 

50mg 1.5mL 100 232062 

100mg 1.5mL 100 232000 

200mg 4.0mL 50 232150 

500mg 4.0mL 50 232300 

Alltech ® Reversed-Phase Ultra-Clean SPE Columns 


Standard C18 

200mg 4.0mL 50 505150 

500mg 8.0mL 30 505355 

Octyl (C8) 

200mg 4.0mL 50 506151 

500mg 4.0mL 50 506251 

500mg 8.0mL 30 506351 

more info 

For SPE Applications, see pages 494–502. 

302 




Normal-Phase Sorbents 

4886 


• Available: Silica (SI), Aminopropyl (NH2), 

Cyanopropyl (CN), Diol (2OH), Florisil ® (FL), 

Florisil ® -PR (FL-PR), Alumina Acidic (AL-A), 

Alumina Acidic (AL-B), Alumina Neutral (AL-N) 




• Available: Silica (SI), Aminopropyl (NH2), 

Florisil ® (FL), Florisil ® -PR (FL-PR) 


applications—fluorinated polypropylene columns with 

PTFE frits 


Functional Group Base 

%. 

Carbon 

End-. 

capped 

Average 

Particle 

Size 

Pore 


Silica (SI) Silica — — 50µm 60Å Highly polar surface Most common polar phase. 

Aminopropyl (NH2) Silica 5.0% No 50µm 60Å Polar phase with slight anion exchange 

properties 

Ideal for carbohydrates or generally with 

analyses containing hydroxyl functional 

groups. 



Diol (2OH) Silica 4.0% No 50µm 60Å Polar surface with minor hydrophobic 

retention 


Magnesium 

Silicate 

— — 75– 

150µm 

60Å Highly polar surface Referenced in many EPA methods. 

Ideally suited for pesticides and metals. 

Florisil ® -PR (FL-PR) Magnesium 

Silicate 

— — 75– 

150µm 

60Å Specifically tested for chlorinated 

pesticides 

Ensures most inert batches suitable for 

highly active compounds. 

Alumina Acidic (AL-A) Aluminum — — 130µm 100Å Alumina washed with acid surface Increase capacity for acidic compounds. 

Oxide 

Alumina Basic (AL-B) Aluminum 

Oxide 

— — 130µm 100Å Alumina washed with base surface Increase capacity for basic compounds. 


Aluminum 

Oxide 

— — 130µm 100Å Alumina washed with neutral surface Interacts with highly aromatic compounds 

and neutral hydroxyls. 

Alltech ® Normal-Phase Extract-Clean Columns 


Silica (SI) 

50mg 1.5mL 100 209062 

100mg 1.5mL 100 209000 

200mg 4.0mL 50 209150 

500mg 4.0mL 50 209250 

500mg 8.0mL 30 209200 

1000mg 8.0mL 30 209100 

2000mg 8.0mL 30 209202 

2000mg 15mL 30 209362 

5000mg 25mL 20 22935 

10,000mg 25mL 20 239300 

10,000mg 75mL 16 239310 

20,000mg 75mL 16 239322 

30,000mg 75mL 16 239330 

Aminopropyl (NH2) 

100mg 1.5mL 100 211000 

200mg 4.0mL 50 211025 

500mg 4.0mL 50 211150 

500mg 8.0mL 30 211256 

1000mg 8.0mL 30 211153 

Cyanopropyl (CN) 

100mg 1.5mL 100 209300 

200mg 4.0mL 50 209450 

500mg 4.0mL 50 209550 

500mg 8.0mL 30 209650 

Diol (2OH) 

100mg 1.5mL 100 208000 

200mg 4.0mL 50 208150 

500mg 4.0mL 50 208250 


100mg 1.5mL 100 204500 

500mg 4.0mL 50 204650 

1000mg 8.0mL 30 207930 

2000mg 15mL 30 207962 

Alltech ® Normal-Phase Extract-Clean Columns (continued) 


Florisil ® (FL) (continued) 

5000mg 25mL 20 227950 

10,000mg 75mL 16 237910 

Florisil ® -PR (FL-PR) 

500mg 4.0mL 50 250015 

1000mg 8.0mL 30 250020 

10,000mg 75mL 16 250910 

Alumina Acidic (AL-A) 

100mg 1.5mL 100 228200 

500mg 4.0mL 50 228350 

Alumina Basic (AL-B) 

100mg 1.5mL 100 228000 

500mg 4.0mL 50 228150 


100mg 1.5mL 100 228400 

500mg 4.0mL 50 228550 

2000mg 15mL 30 22856 

Alltech ® Normal-Phase Ultra-Clean SPE Columns 


Silica (SI) 

200mg 4.0mL 50 509150 

500mg 8.0mL 30 509225 

5000mg 25mL 20 509010 

Aminopropyl (NH2) 

200mg 4.0mL 50 511025 

500mg 4.0mL 50 511150 


500mg 4.0mL 50 507851 

1000mg 8.0mL 30 507930 

Florisil ® -PR (FL-PR) 

500mg 4.0mL 50 504651 

1000mg 8.0mL 30 507900 





Ion-Exchange Sorbents 

4886 


• Available: SCX, SAX, IC-OH, IC-H, IC-Ag, 

IC-Ba, IC-Na, IC-Chelate, IC-RP 




• Available: SCX, SAX 




Ion-Exchange Sorbent Specifications 


Particle 

Size 

SCX Styrene- 

DVB 

SAX 

Styrene- 

DVB 

Functional 

Group 


Sulfonic 

Acid 


Ammonium 

Exchange 


2.0meq/mL Cations, (+) charged compounds Remove/concentrate basic compounds 

1.0meq/mL Anions, (–) charged compounds Remove/concentrate basic compounds 





DVB 

IC-H 

IC-Ag 

IC-Ba 

IC-Na 

IC-Chelate 

Styrene- 

DVB 

Styrene- 

DVB 

Styrene- 

DVB 

Styrene- 

DVB 

Styrene- 

DVB 

Particle 

Size 

Molecular 

Exclusion 

Limit 


Daltons 


Daltons 


Daltons 


Daltons 


Daltons 


Daltons 

Exchange 










samples. 












IC-RP Polystyrene — 550µm — — Hydrophobic components Removes surfactants, organic acids, and 


pass through. 

General Ion-Exchange Extract-Clean Columns 


SCX 

100mg 1.5mL 100 209800 

200mg 4.0mL 50 209825 

500mg 4.0mL 50 209950 

1000mg 8.0mL 30 209930 

SAX 

100mg 1.5mL 100 209600 

200mg 4.0mL 50 209625 

500mg 4.0mL 50 209750 

1000mg 8.0mL 30 209850 

General Ion-Exchange Ultra-Clean Columns 


SCX 

200mg 4.0mL 50 509826 

500mg 4.0mL 50 509951 

SAX 

200mg 4.0mL 50 509626 

500mg 4.0mL 50 509751 

Ion Chromatography Extract-Clean Columns 

Packing Bed Weight Column Size Qty. Part No. 

IC-OH 0.5mL 4.0mL 50 40262 

IC-OH 1.5mL 4.0mL 30 140254 

IC-H 0.5mL 4.0mL 50 40264 

IC-H 1.5mL 4.0mL 30 140256 

IC-Ag 0.5mL 4.0mL 50 105050 

IC-Ag 1.5mL 4.0mL 30 140258 

IC-Ba 0.5mL 4.0mL 50 40268 

IC-Ba 1.5mL 4.0mL 30 140261 

IC-Na 0.5mL 4.0mL 50 40270 

IC-Na 1.5mL 4.0mL 30 140263 

IC-Chelate 0.5mL 4.0mL 50 40250 

IC-Chelate 1.5mL 4.0mL 30 140265 

IC-RP 0.5mL 4.0mL 50 40260 

IC-RP 1.5mL 4.0mL 30 140252 

more info 


304 




Specialty Sorbents 

4886 


• Available: DVB, Carbograph, AFT, Filter, 

Phase Separator, Drying 




• Available: Carbograph 




Specialty Packings Specifications 

%. 

Carbon 

End-. 

capped 

Average 

Particle 

Size 

Pore 


Packing 

Base 

DVB 100% DVB — — 40µm — 100% DVB Greater capacity than C18 for general 

SPE. Also free vinyl surface groups make 

a suitable solid-phase synthesis support. 

Carbograph 

AFT 

Graphitized 

Carbon 

C18, Silica, 

and Alumina 

— — 38– 

125µm 

6 — 50– 

130µm 

— Graphitized carbon Retains polar organics in aqueous 

matrices. Ideally suited for acid, baseneutral 

extraction of pesticides and 

herbicides. 

Unique blend of reversed and normal Ideal for Aflatoxins. 

phases 

DVB Extract-Clean Columns 

• Greater sample capacity than C18 

• 100% divinylbenzene reduces swelling 

• 40µm average particle size 

DVB Extract-Clean Columns 


25mg 1.5mL 100 220025 

50mg 1.5mL 100 220050 

100mg 1.5mL 100 220100 

500mg 4.0mL 50 220500 

Carbograph Extract-Clean Columns 

• Graphitized carbon retains polar organics in aqueous 

matrices 

• Acid, base-neutral extraction of pesticides and herbicides 

• 100m 2 /g surface area 

Carbograph Extract-Clean Columns 


150mg 4.0mL 50 210142 

300mg 8.0mL 30 210101 

500mg 8.0mL 30 210150 

1000mg 15mL 20 210121 

Carbograph Ultra-Clean Columns 


150mg 4.0mL 50 510142 

300mg 8.0mL 30 510101 

AFT Extract-Clean TM Columns 

This blend of C18 and Alumina materials offers unique 

selectivity for Mycotoxin removal. AFT Tubes are ideal for 

food and beverage analysis. 

Phase Separator Extract-Clean TM Columns 

Use for fast and simple separation of organic and aqueous 

mixed samples. The column contains a 20µm polyethylene 

frit and a hydrophobic silicone membrane that allows the 

hydrophobic phase to pass through, while the aqueous phase 

is retained in the upper chamber. 

Phase Separator Extract-Clean TM Columns 

Column Size Qty. Part No. 

4.0mL 100 205289 

8.0mL 100 205389 

25mL 100 205589 

Filter Columns 

Filter columns are Extract-Clean reservoirs with two frits at 

the outlet end. They remove particulate matter down to 20µm 

from samples. Syringe adapters will connect filter columns to 

the tops of Extract-Clean columns. 

Extract-Clean Filter Columns 


1.5mL Filter Columns 100 211101 




Drying Tubes 

Packed with anhydrous sodium sulfate, use these to remove 

residual water from SPE extracts. They are suitable for 

pesticide analysis. 

Drying Tubes 

Bed Weight Qty. Part No. 

Extract-Clean Column, 3g 100 219002 


AFT Extract-Clean TM Columns 


8.0mL 1000mg 100 229101 



Alltech ® Extract-Clean Columns 

Specialty Columns 

Drug-Clean PB Mixed-Mode SPE Columns 

• Polymer based 

• Extract acidic, basic, and neutral compounds from 

one column 

• Stable from pH 1 to 14 

• No conditioning steps required 

• Faster flow rates than silica-based materials 

Drug-Clean PB has a C18/SCX cation function on a highly 

cross-linked styrene divinylbenzene base. The high sorbent 

capacity greatly reduces the amount of packing required 

for separation and makes this material ideal for use in 

96-well plates—see page 311. 

Each box of columns includes extraction methods for 

amphetamines, opiates, cocaine/benzoylecgonine, 

phencyclidine, and carboxy-THC. 

Drug-Clean SB Mixed-Mode SPE Columns 

• Silica based 

• Efficient—higher recoveries with minimal impurities 

compared to single mode extractions 

• Multi-functional—extract acidic, neutral, and basic 

compounds with a single SPE bed 

• Reproducible—true copolymer is cleaner and more 

accurate than mixed beds 

Drug-Clean C is a C8/SCX cation function. Retain neutral 

or amine containing compounds while carboxylate groups 

pass through. Drug-Clean A is a C8/SAX anion function. 

Retain neutral or carboxylate compounds while amine group 

retention is minimized. 

Each box of columns includes extraction methods for the most 

popular NIDA drugs of abuse. 

Drug-Clean PB Specifications 

Functional Group: C18/Cation Function 

Base: 

30µm Styrene/DVB Polymer 

Retention Mechanism: Mixed-mode, reversed-phase, and ion-exchange 

Drug-Clean SB Specifications 

Functional Group: C8/Ion-Exchange 

Base: 

50µm irregular silica, 60Å 

Retention Mechanism: Mixed-mode, reversed-phase, and ion-exchange 


Drug-Clean PB SPE Columns 


30mg 1.5mL 100 250120 

30mg 4.0mL 50 250130 

50mg 8.0mL 50 250140 

1 

2 

Cocaine and Benzoylecgonine 

Extract from Urine 

4 5 6 7 8 9 10 11 12 13 14 Min. 

2656 

1. Cocaine (2.5mg/mL) 

2. Benzoylecgonine-TMS, 

Benzoylecgonine-d3-TMS 

(5.0mg/mL) 

Drug-Clean SB Columns 


Drug-Clean C (Mixed-Mode Cation) Columns 

100mg 1.5mL 100/pk 207015 

200mg 4.0mL 50/pk 207010 

500mg 4.0mL 50/pk 207017 

500mg 8.0mL 30/pk 207019 

Drug-Clean A (Mixed-Mode Anion) Columns 

100mg 1.5mL 100/pk 207030 

200mg 4.0mL 50/pk 207034 

Amphetamines Extract from Urine 

1 

2 

2673 

1. Amphetamine (2.5 mg/mL) 

2. Methamphetamine (2.5 mg/mL) 


Capillary GC Column (Part No. 15807) 

Temp: 

225°C (0min hold) to 315°C (11min hold) 

at 10°C/min 

Carrier Gas: Helium at 0.8mL/min (28cm/sec) 

Detector: MSD at 325°C 

Sample Prep: Drug-Clean C 

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 Min. 



Temp: 

180°C to 300°C at 10°C/min 



tech tip 

Why is a “True Copolymer” better than a mixed bed? 

By definition, a mixed-mode packing contains two different functional groups on the same sorbent. To minimize costs, two individual 

packings are sometimes blended together physically and packed in a tube. This gives the effect of a mixed-mode but in fact is a 

mixed-bed packing with batch-to-batch reproducibility dependent on the blending skills of the manufacturer. A true mixed-mode has the 

functional groups polymerized on the same silica base. This copolymer treatment assures a more predictable packing performance 

which means more reproducible results from tube to tube, batch to batch. 

306 




Save Time and Maximize Sample Preparation Efficiency 

Maxi-Clean cartridges have the same bed dimensions as 4mL SPE columns for easy method cross-over. Process a 

single cartridge by syringe or multiple cartridges by vacuum. Maxi-Clean cartridges allow you to stack different cartridges for 

multi-step extractions. Use top and bottom caps for easy transport of field samples. Here’s 10 different ways these cartridges can 

be used to address difficult extractions and SPE protocols. 

1. Bulk Preconditioning on a Vacuum. 

Manifold 

Save time and solvents by 

preconditioning Maxi-Clean 

Cartridges on a vacuum manifold. 

Double stack for even faster 

preparation. 

6080 

6. Easy to Transport and Store 

Use top and bottom caps to transport or store 

a Maxi-Clean Super Cartridge containing 

your analyte, without risking contamination or 

dehydration. 

6086 

2. Direct Substitution into Methods Using . 

Standard 4mL Columns 

Maxi-Clean Cartridges have the 

same bed dimensions as traditional 

4mL SPE columns, for easy 

substitution into established protocols. 

6085 

7. Process Individually or in Parallel 

Do you only have a few samples 

to process? Process them with 

a syringe, instead of setting up 

a vacuum manifold. Do you have 

a lot of samples to process? Add 

an empty reservoir to process them 

simultaneously on your manifold. 

3. Stack Two Cartridges in Series . 

for Complex Separations 

6087 

Maxi-Clean Cartridges can be stacked 

to combine two phases into one extraction 

step for complex analyses. You can even 

elute each phase individually, if desired. 

4. Elute Anywhere 

Use a syringe to elute your 

analyte anywhere—directly 

into an injection valve, or into 

other receiver vessels. You can 

even attach a needle and elute 

directly through a septa or 96-well sealing mat. 

5. Combine SPE with Filtration by Adding a . 

Filter Tube or Syringe Filter in Series 

6082 

6083 

8. Use In-line 

The Maxi-Clean cartridge’s luer connections 

allow you to use them in-line, to remove 

contaminants or act as a “guard” cartridge 

in low-pressure applications. 

9. Custom-fit to Your Sample Size 

By changing reservoir sizes, you 

can customize a Maxi-Clean 

Cartridge to fit your sample 

size without changing the bed 

dimensions. 

6091 

6088 


The Maxi-Clean Cartridge’s luer 

connections allow you to add a syringe filter 

or a fritted SPE tube to remove particulates 

before your sample enters the SPE bed. 

10. Choose from 23 Chemistries 

6089 

The Maxi-Clean line is offered 

in many of the same media as 

the Extract-Clean line, but 

slightly paired down, with over 

20 chemistries available. 

6091 

6084 

6090 




Reversed-Phase Cartridges 

• Same bed dimensions as 4mL SPE columns for 

method cross-over 

• Process a single cartridge by syringe or multiple 

cartridges by vacuum 

• Stack different cartridges for multi-step extractions 

4881 

• Use top and bottom caps for easy transport of 

field samples 4915 


%. 

Carbon 

End-. 

capped 

Average 

Particle 

Size 

Pore 


Packing 

Base 

Prevail C18 Silica 11.0% Yes 50µm 60Å 100% water wettable Hydrophilic/hydrophobic retention. Phase 

remains active even when completely dry. 

Can omit preconditioning step. 

Standard C18 Silica 6.0% Yes 50µm 60Å Low carbon load C18 General purpose phase. 

High-Flow C18 Silica 8.0% Yes 100µm 60Å Large particle Less flow resistance for faster flow rates 

of large volume sample. 

High-Capacity C18 Silica 17.0% Yes 50µm 60Å High carbon load Maximum capacity phase. 

Large Pore C18 Silica 14.0% Yes 50µm 150Å Larger than average pore size Ideal for compounds >1500MW. 

Octyl (C8) Silica 4.5% Yes 50µm 60Å Less hydrophobic than C18 Less retention of highly hydrophobic 


retentive. 

Ethyl (C2) Silica 5.5% Yes 50µm 60Å Short chain functional group is less 

hydrophobic than C8 

Less retention of highly hydrophobic 


retentive. 

Phenyl (PH) Silica 3.8% Yes 50µm 60Å Aromatic structure Highly selective for aromatic compounds. 


Maxi-Clean Reversed-Phase Cartridges 

Packing Bed Weight Qty. Part No. 

Prevail C18 300mg 50 605926 

500mg 50 605929 

900mg 50 605942 

Standard C18 300mg 25 20924 

300mg 50 20926 

300mg 100 20928 

600mg 25 20932 

600mg 50 20934 

600mg 100 20936 

900mg 25 20940 

900mg 50 20942 

900mg 100 20944 

High-Capacity C18 300mg 50 20945 

Large Pore C18 300mg 100 22012 

600mg 100 22017 

900mg 100 220215 

Octyl (C8) 300mg 50 20950 

600mg 50 20958 

900mg 50 20966 

Ethyl (C2) 300mg 50 210064 

Phenyl (PH) 300mg 25 22002 

300mg 50 22003 

300mg 100 22004 

tech tip 

What is a Maxi-Clean cartridge? 

A Maxi-Clean cartridge is an alternative format for SPE. It uses 

the same high-quality packing materials as the Extract-Clean 

columns but has a polypropylene housing with both a female 

luer inlet and a male luer outlet tip. This allows use of positive 

pressure from a syringe or negative pressure from a vacuum 

manifold. 20µm polyethylene frits are placed at each end of the 

sorbent bed. The packing material is packed and compressed to 

improve or optimize flow characteristics. 

4870 

more info 



Looking for vacuum manifolds? 

See pages 314–315 for vacuum 

manifolds to process samples. 

Maxi-Clean Drying Cartridges 


Sodium Sulfate 3g 100 219001 

308 




Normal-Phase Cartridges 

• Same bed dimensions as 4mL SPE columns for 

method cross-over 

• Process a single cartridge by syringe or multiple 

cartridges by vacuum 

• Stack different cartridges for multi-step extractions 

4881 

• Use top and bottom caps for easy transport of 

field samples 4915 


Packing 

Base 

%. 

Carbon 

End-. 

capped 

Average 

Particle 

Size 

Pore 


Silica (SI) Silica — — 50µm 60Å Highly polar surface Most common polar phase. 

Aminopropyl (NH2) Silica 5.0% No 50µm 60Å Polar phase with slight anion exchange 

properties 

Ideal for carbohydrates or generally with 

analyses containing hydroxyl functional 

groups. 



Diol (2OH) Silica 4.0% No 50µm 60Å Polar surface with minor hydrophobic 

retention 

Wets easily and offers more 

reproducibility. 


Magnesium 

Silicate 

— — 75– 

150µm 

60Å Highly polar surface Referenced in many EPA methods. 

Ideally suited for pesticides and metals. 

Florisil ® -PR (FL-PR) Magnesium 

Silicate 

— — 75– 

150µm 

60Å Specifically tested for chlorinated 

pesticides 

Ensures most inert batches suitable for 

highly active compounds. 

Alumina Acidic (AL-A) Aluminum — — 130µm 100Å Alumina washed with acid surface Increase capacity for acidic compounds. 

Oxide 

Alumina Basic (AL-B) Aluminum 

Oxide 

— — 130µm 100Å Alumina washed with base surface Increase capacity for basic compounds. 


Aluminum 

Oxide 

— — 130µm 100Å Alumina washed with neutral surface Interacts with highly aromatic compounds 

and neutral hydroxyls. 

Maxi-Clean Normal-Phase Cartridges 


Silica (SI) 300mg 25 20972 

300mg 50 20974 

300mg 100 20976 

600mg 25 20980 

600mg 50 20982 

600mg 100 20984 

900mg 25 20988 

900mg 50 20990 

900mg 100 20992 

Aminopropyl (NH2) 300mg 50 210044 

300mg 100 210046 

900mg 100 210047 

Cyanopropyl (CN) 300mg 50 210034 

300mg 100 210036 

Diol (2OH) 300mg 50 210084 

300mg 100 210086 

Florisil ® (FL) 300mg 50 210054 

300mg 100 210056 

900mg 50 210057 

900mg 100 210061 

Florisil ® -PR (FL-PR) 300mg 50 210074 

300mg 100 210076 

900mg 50 210079 

900mg 100 210075 

Alumina Acidic (AL-A) 300mg 25 210094 

1800mg 25 210097 

Alumina Basic (AL-B) 300mg 25 210093 

1800mg 25 210096 

Alumina Neutral (AL-N) 300mg 25 210095 

1800mg 25 210098 


Looking for standard . 

luer-hub syringes to 

process Maxi-Clean 

cartridges? 

See page 347. 





4675 





Ion-Exchange Cartridges 

• Eliminate matrix interferences before ion analysis 

• Seven chemistries solve a variety of specific problems 

The most difficult part of many IC applications is eliminating interfering 

components from the sample matrix. These interferences may co-elute or mask 

peaks of interest, overload the column, or shorten the column life by binding 

irreversibly to the column packing. The Alltech ® unique ion-exchange SPE 

cartridge eliminates many of these interferences 1,2 . 

Each metal-free polypropylene cartridge contains 0.5mL or 1.5mL of purified polystyrene resin, contained by 

20µm polyethylene frits. The resin is functionalized to retain specific types of components from the sample. 

1 

R. Saari-Nordhaus, J.M. Anderson, Jr., and I.K. Henderson, Am. Lab. August (1990) 19. 

2 

I.K. Henderson, R. Saari-Nordhaus, and J.M. Anderson, Jr., J. Chromatogr. 546 (1991) 61. 

4893 

Ion-Exchange Sorbent Specifications 


Particle 

Size 

SCX Styrene- 

DVB 

SAX 

Styrene- 

DVB 

Functional 

Group 


Sulfonic 

Acid 


Ammonium 

Exchange 


2.0meq/mL Cations, (+) charged compounds Remove/concentrate basic compounds. 

1.0meq/mL Anions, (–) charged compounds Remove/concentrate basic compounds. 





DVB 

IC-H 

IC-Ag 

IC-Ba 

IC-Na 

IC-Chelate 

Styrene- 

DVB 

Styrene- 

DVB 

Styrene- 

DVB 

Styrene- 

DVB 

Styrene- 

DVB 

Particle 

Size 

Molecular 

Exclusion 

Limit 


Daltons 


Daltons 


Daltons 


Daltons 


Daltons 


Daltons 

Exchange 










samples. 












IC-RP Polystyrene — 550µm — — Hydrophobic components Removes surfactants, organic acids, and 


pass through. 

General Ion-Exchange Maxi-Clean Cartridges 


SCX 600mg 25 21901 

600mg 50 21902 

600mg 100 21903 

SAX 600mg 25 21906 

600mg 50 21907 

600mg 100 21908 

Ion Chromatography Maxi-Clean Cartridges 


IC-OH 0.5mL 50 30262 

IC-OH 1.5mL 25 30254 

IC-H 0.5mL 50 30264 

IC-H 1.5mL 25 30256 

IC-Ag 0.5mL 50 30266 

IC-Ag 1.5mL 25 30258 

IC-Ba 0.5mL 50 30268 

IC-Ba 1.5mL 25 30261 

IC-Na 0.5mL 50 30270 

IC-Na 1.5mL 25 30263 

IC-Chelate 0.5mL 50 30250 

IC-Chelate 1.5mL 25 30265 

IC-RP 0.5mL 50 30260 

IC-RP 1.5mL 25 30252 

Mixed-Mode RP-OH 0.5mL 25 30259 

Mixed-Mode RP-H 0.5mL 25 30267 

310 



Alltech ® 96-Well Plates 

• High-throughput SPE devices 

• Standard 96-Well format 

• Square 2mL wells 

Prevail C18 96-Well Plates 

Bed Weight Well Volume Qty. Part No. 

25mg 2.0mL 1 8619193 

50mg 2.0mL 1 8619192 

100mg 2.0mL 1 8619191 

4888 

SAX 96-Well Plates 


25mg 2.0mL 1 219696 

50mg 2.0mL 1 209696 

100mg 2.0mL 1 229696 

Standard C18 96-Well Plates 


25mg 2.0mL 1 214996 

50mg 2.0mL 1 204996 

100mg 2.0mL 1 224996 

Silica 96-Well Plates 


25mg 2.0mL 1 219096 

50mg 2.0mL 1 209096 

100mg 2.0mL 1 229096 

SCX 96-Well Plates 


25mg 2.0mL 1 219896 

50mg 2.0mL 1 209896 

100mg 2.0mL 1 229896 

Drug-Clean 96-Well Plate 


30mg per Well ea 250150 


Looking for empty . 

96-Well plates? 


Vydac ® SPE Columns 

For Extraction, Concentration and Clean-up of Biological Samples 

Vydac ® SPE columns are disposible sample clean-up 

devices which complement Grace Vydac ® HPLC columns. 

The media are the same high-quality silica bonded with the 

same chemistries used in Vydac ® 300Å TP reversed-phase 

HPLC columns, giving Vydac ® SPE columns similar selectivity 

and recovery. 

Applications for Vydac ® SPE columns: 

• Desalting of polypeptide solutions 

• Concentration of proteins and peptides 

• Removal of HF and cleavage products from cleavage 

solutions 

• Removal of lipids and strongly bound proteins 

• Improvement of HPLC resolution by prior removal of 

early and late eluting by-products or reagents 

• Preparation of environmental and food samples 

Vydac ® SPE Columns 

Column Size Pkg. Part No. 

C4, 13µm 1 mL 50 214SPE1000 

3 mL 50 214SPE3000 

C18, 13µm 1 mL 50 218SPE1000 

3 mL 50 218SPE3000 

Solid-Phase Extraction Example 

Column: 

Gradient: 

A. 5% ACN 

B. 30% ACN 

ribonuclease 

C. 60% ACN 

myoglobin 

0 10 Min. 

Vydac ® 214TP5405, C4, 5µm, 4.6 x 50mm 

15 to 70% ACN over 10min, with 0.1% TFA 

A 3mL SPE column was conditioned with 1mL of ACN followed by 

1mL of 5% ACN/0.1% TFA. Ribonuclease and myglobin (100mg each) 

were then loaded in 30% ACN/0.1% TFA. The column was washed with 

1mL of 5% ACN/0.1% TFA to remove weakly bound compounds, then 

1mL of 30% ACN/0.1% TFA followed by 1mL of 60% ACN/0.1% TFA. 

HPLC analysis of the 5% ACN wash (A) revealed only a small amount 

of ribonuclease. Most of the ribonuclease eluted in the 30% ACN wash 

(B). The myoglobin eluted almost entirely in the 60% ACN wash (C). 




GracePure SPE Sorbent Specifications 

• Ideal for cost-conscious labs 

• Concise offering of popular sorbents 

new 

GracePure SPE products have a concise offering of sorbents suitable for 

a variety of applications. Whether pharmaceutical or petrochemical, these products 

deliver the selectivity and high recovery you expect. Use this guide to help you choose 

the appropriate sorbent, bed size, and solvent volumes to ensure you have a cleaner, more 

concentrated sample at the end of your SPE process. 

Reversed-Phase (Non-Polar) Sorbents Specifications 

% 

Carbon 

Surface. 

Area 

Particle. 

Size 

Sorbent Support 

Endcapping 

C18-Max Silica 17.1% Yes 518m 2 /g 50µm 60Å Polymerically bonded 

17% carbon load 

Pore. 

Size Feature Benefit Application Type 

Highest binding capacity, 

best for complex samples or 

structurally diverse analytes. 

C18-Aq Silica 12.5% Yes 518m 2 /g 50µm 60Å Hydrophilic endcapping Water-wettable C18 ideal for 

aqueous samples. Phase 

remains active even when 

completely dry. 

C18-Low Silica 6.5% Yes 518m 2 /g 50µm 60Å Least hydrophobic C18 phase that easily releases 

C18 phase 

very hydrophobic compounds. 

C18-Fast Silica 7.0% Yes 518m 2 /g 100µm 60Å Large 100µm particle Process large volume (>500mL) 

or viscous samples with fast 

flow rates. 

TMS Silica 5.6% No 518m 2 /g 50µm 60Å Low carbon load 

trimethyl silane phase 

Least hydrophobic reversedphase 

elutes non-polar 

compounds easily. Short carbon 

chain has little steric hindrance 

to uniformly cover silica surface. 

Drugs and their metabolites in 

serum and plasma, pesticides 

Desalting proteins, 

pharmaceuticals, hormones, 

pesticides, organics in water 

Surfactants, oils, antibiotics 

Aniline, pesticides, haloethers, 

phthalate esters, EPA 3620, 

3610 

Oils, dyes, surfactants 


Normal-Phase (Polar) Sorbents Specifications 


% 

Carbon Endcapping 

Surface 

Area 

Particle 

Size 

Pore 


Silica Silica N/A N/A 518m 2 /g 50µm 60Å Most polar phase Able to differentiate between 

structurally similar compounds. 

Aflatoxins, pesticides, 

steroids, structural isomers 

Amino Silica 4.3% No 518m 2 /g 50µm 60Å Dual retention Retains polar compounds, 

or can act as a weak anion 

exchanger. Easily releases 

strong acids when SAX binds 

too strongly. 

Diol Silica N/A No 518m 2 /g 50µm 60Å Reproducible polar 

bonded phase 

Florisil ® 

Magnesium 

Silicate 

N/A No 120µm 60Å Alternates polar 

phase 

Very polar phase that has the 

same benefits as silica, but 

wets easily and offers more 

reproducibility. 

Large particle size processes 

large sample sizes quickly. 

Carbohydrates, dyes, lipids, 

mycotoxins, strong acids 

Alkaloids, lipids, oils, structural 

isomers 

Environmental 

Ion-Exchange Sorbents Specifications 

Exchange 


Capacity Counter Ion 

Anion-X 8% cross-linked 

styrenedivinylbenzene 

Cation-X 

8% cross-linked 

styrenedivinylbenzene 

Particle 


1.5meq/g Acetate form 50µm Tetramethyl ammonium 

functional group on 

polymer base material 

2.4meq/g Hydrogen form 50µm Benzene sulfonic acid 

functional group on 

polymer base material 

pH range from 1–14, with 

excellent exchange capacity. 

pH range from 1–14, with 

excellent exchange capacity. 

Anionic compounds: organic 

acids, fatty acids 

Cationic compounds: amines, 

amino acids 

312 



GracePure SPE Columns 

new 

Quality Assurance Certificate 

Base Material Properties 

GracePure base silicas are characterized multiple 

ways to ensure that the starting point for every batch 

of media is consistent. Parameters that can directly affect 

SPE results are shown for lot-to-lot comparison. 

Sorbent Characterization 

Tight specifications promise clean and reproducible sorbent 

performance. An HPLC chromatogram offers a detailed look 

at selectivity that is not possible with recovery tests alone. 

Turbidity measurements after sorbent bonding confirm that 

fines were not created during the manufacturing process. 

Component Tests 

GC/FID shows that GracePure tubes are constructed from 

a highly inert grade of polypropylene to prevent extractable 

contamination. Polyethylene frits are thoroughly washed in 

organic solvent which also eliminates extractables. 

Manufacturing Control 

GracePure SPE products are packed and assembled using 

custom-designed, precision equipment. Every manufacturing 

batch is guaranteed to have less than 2% bed weight variation 

and uniform flow rates. A sophisticated visual inspection 

system only accepts product that meets our high standards 

for bed consistency and frit integrity. 

GracePure Reversed-Phase SPE Columns 

Sorbent Bed Weight Column Volume Qty. Part No. 

C18-Max 50mg 1mL 100pk 5141484 

100mg 1mL 100pk 5138765 

500mg 3mL 50pk 5138766 

500mg 6mL 30pk 5138767 

1000mg 6mL 30pk 5138768 

2000mg 20mL 20pk 5141525 

5000mg 20mL 20pk 5141524 

C18-Aq 50mg 1mL 100pk 5141486 

100mg 1mL 100pk 5138774 

500mg 3mL 50pk 5138775 

1000mg 6mL 30pk 5138776 

2000mg 12mL 30pk 5141482 

5000mg 20mL 20pk 5141523 

5000mg 20mL 30pk 5141489 

C18-Low 100mg 1mL 100pk 5138760 

200mg 3mL 50pk 5138761 

500mg 3mL 50pk 5138762 

500mg 6mL 30pk 5138763 

1000mg 6mL 30pk 5138764 

5000mg 20mL 20pk 5141526 

C18-Fast 500mg 3mL 50pk 5138758 

1000mg 6mL 30pk 5138759 

5000mg 20mL 20pk 5141527 

TMS 50mg 1mL 100pk 5141485 

100mg 1mL 100pk 5138785 

500mg 3mL 50pk 5138786 

GracePure Normal-Phase SPE Columns 


Silica 100mg 1mL 100pk 5138777 

200mg 3mL 50pk 5138778 

500mg 3mL 50pk 5138779 

5000mg 20mL 20pk 5138780 

500mg 6mL 30pk 5138781 

1000mg 6mL 30pk 5138782 

2000mg 12mL 30pk 5138783 

10000mg 60mL 16pk 5138784 

Amino 500mg 3mL 50pk 5138752 

1000mg 6mL 30pk 5138753 

Diol 100mg 1mL 100pk 5138771 

200mg 3mL 50pk 5138772 

500mg 3mL 50pk 5138773 

Florisil ® 500mg 3mL 50pk 5141522 

1000mg 6mL 30pk 5141520 

2000mg 12mL 30pk 5141521 

GracePure Ion-Exchange SPE Columns 


Anion-X 100mg 1mL 100pk 5138754 

500mg 3mL 50pk 5138755 

1000mg 3mL 50pk 5141487 

Cation-X 100mg 1mL 100pk 5138769 

500mg 3mL 50pk 5138770 

1000mg 3mL 50pk 5141488 


more info 




SPE Tube 

Vacuum Manifolds 

• 12-, 16-, and 24-port manifolds 

• Glass chamber for visual 

monitoring 

• Accepts standard male luer 

devices 

I 

C 

Vacuum Manifold Components 

A. Glass Chamber 

B. Vacuum Valve and Gauge 

C. Polypropylene Lid 

D. Stopcock Valves 

E. Collection Rack Plates 

F. Support Posts for Collection Racks 

G. Retaining Clips for Collection Racks 

H. Lid Legs 

I. Manifold Inlet Caps 

J. Polypropylene Needles 

Vacuum manifolds process multiple 

samples simultaneously, saving 

time and effort. Manifold systems 

come complete with the components 

listed below. Stainless steel or PTFE 

needles are available separately. 

A 

E 

F 

H 

B 

E 

J 

I 

G 

D 

6278 

12-Port Manifold 

• For up to 12 samples 

• Also includes one waste 

container 

24-Port Manifold 

• Process up to 24 samples 

4871 


12-Port Manifold and Accessories 


12-Port Vacuum Manifold** ea 210351 


Lid, Gaskets, and 12 Stopcocks ea 212001 

Glass Chamber ea 213212 

Vacuum Gauge, Valve, and Glass Chamber ea 212304 

Collection Rack, 12-Port Size* ea 212518 

Gaskets, 12-Port Size 2 212112 

One-Way Stopcocks 12 213112 

Waste Container 2 210033 

*12-Port Collection Rack includes 3 support posts, bottom plate, 13- and 16mm plates, 

autosampler vial plate, volumetric plate, and 12 retaining clips. 

**Includes waste container. 

96-Well Plate 

Vacuum Manifold 

4869 

24-Port Manifold and Accessories 


24-Port Vacuum Manifold ea 210224 


Lid, Gaskets, and 24 Stopcocks ea 211224 

Glass Chamber ea 210124 

Vacuum Gauge, Valve, and Glass Chamber ea 210324 

Collection Rack, 24-Port Size † ea 210424 

Gaskets, 16- and 24-Port Size 2 210724 

One-Way Stopcocks 24 211524 

† 

16- and 24-Port Collection Racks include 3 support posts, bottom plate, dimple plate, 

13- and 16mm plates, and 12 retaining clips. 

Process standard 2mL 96 Deep Well Plates via vacuum 

source. Collect directly into a microplate. Also accommodates 

48 4mL SPE columns or 24 6mL SPE columns with 

appropriate plate adapters. 

96-Well Plate Vacuum Manifold 


96-Well Plate Vacuum Manifold ea 8619194 

7435 

314 



Manifold Accessories 

Replacement Parts for All Size Manifolds 

Manifolds come complete with all necessary gauges and 

accessories. Individual replacement parts may be sold 

separately. 

Drying Attachments 

Use these special drying lids to direct a gas flow into 

collection tubes to dry eluents. Barb is 1/4". 

B 

F 

G 

D 

E 

A 

C 

5951 

4878 

Replacement Parts for All Size Manifolds 


A. Vacuum Gauge and Valve ea 212203 

B. Retaining Clips for Collections Racks 12 212912 

C. Female Luer Inlet 2 212002 

Female Luer Inlet 24/pk 212302 

D. Male Luer Outlet 2 212120 

Male Luer Outlet 24/pk 212320 

E. Caps for Lid Inlets 50 211234 

F. Lid Legs, Black 4 410510 

G. Collection Rack Posts 3 410410 

Tygon ® Tubing 

Tygon ® vacuum tubing is heavy walled and flexible. 

It withstands 29.9" Hg at 75°F (24°C). It is nontoxic, 

nonoxidizing, and transparent. 

Drying Attachments 


12-Port Drying Attachment 1 212100 



Manifold Needles 

PTFE needles are disposable, fit many different manifold 

types, and eliminate cross contamination by extending into 

the collection tube. They also provide a complete PTFE fluid 

path for samples to virtually eliminate extractables. Stainless 

steel and polypropylene needles are also available. 


PTFE 

Polypropylene 


Valved PTFE 

4874 

Tygon ® Vacuum Tubing 


1/8" i.d., 3/8" o.d. 50' 6465 

3/16" i.d., 9/16" o.d. 10' 6467 

1/4" i.d., 3/4" o.d. 10' 6469 

3/8" i.d., 7/8" o.d.* 10' 6472 

*Use with Vacuum Gauge and Valve displayed above. 

3849 

Manifold Needles 


PTFE Needles 100 412410 

PTFE Needles 500 412450 

Valved PTFE Needles 25 411525 

Valved PTFE Needles 50 411550 

Stainless Steel Needles 12 212400 



Polypropylene Needles 12 212412 





Reservoirs and Frits 

Select empty reservoirs and loose frits to pack your own 

custom SPE columns. 

Adapters and Caps 

• Inlet caps fit SPE devices as indicated 

• Outlet caps fit any column or cartridge with a male luer tip 

5952 


Bulk Reservoirs and Frits 


Extract-Clean Empty Reservoirs, Polypropylene 

1.5mL 100 210001 

4.0mL 100 210104 

8.0mL 100 210208 

15.0mL 100 210315 

25.0mL 100 210425 

75.0mL 50 210575 

Ultra-Clean Empty Reservoirs, Treated Polypropylene 

4.0mL 100 70216 

8.0mL 100 70218 

8.0mL 1000 70069 

Polyethylene Frits for Extract-Clean Reservoirs 

For 1.5mL Reservoir 100 211401 

For 4.0mL or EV Reservoir 100 211404 





PTFE Frits for Ultra-Clean Reservoirs 



Empty 96-Well Plates 

• Square, 2mL wells 

• All-polypropylene construction 

• Use to pack your own custom 

SPE plates 

6288 

Standard 96-well plate 

footprint (8x12 wells) 

96-Well Plates 


Empty plate with bottom frit installed, 

top frit supplied 

1 210780 

4888 

Caps 


Inlet Caps for Maxi-Clean Cartridges 50 211015 

Inlet Caps for 1.5mL 50 220000 






Outlet Caps for Male Luers 50 220710 

Syringe Adapters fit the tops of SPE columns allowing 

attachment of any male luer device. This lets you process 

an Extract-Clean column with a syringe or add an empty 

reservoir to increase sample volume capacity. 

Syringe Adapters 


Syringe Adapters for 1.5, 4.0, and 8.0mL 

15 210705 

Columns 

Syringe Adapters for 25mL Columns 5 210707 

Syringe Adapters for 75mL Columns 5 210709 

Bulk SPE Packings 

The same high-quality material we use to 

manufacture our Extract-Clean and 

Maxi-Clean SPE products. 

Bulk SPE Packings 


Reversed-Phase Adsorbents 

Prevail C18 (11%) 100g 211505 

C18 (6%) 100g 211502 

C18 (17%) 100g 211503 

Large Pore C18 (14%) 100g 211542 

C8 100g 211504 

C2 100g 211506 

Phenyl 100g 211522 

Normal-Phase Adsorbents 

Silica 100g 211512 

Aminopropyl 100g 211516 

Diol 100g 211518 

Florisil ® 227g 5618 

Florisil ® -PR 227g 5615 

Alumina-N 100g 211541 

Ion-Exchange 

SCX (Cation Exchange) 100g 211520 

SAX (Anion Exchange) 100g 211510 

Carbograph 1 SPE 120/400 Mesh 25g 1769 

4900 

316 



Filtration Introduction 

Filter Membrane Chemical Compatibility 

C = Compatible 

L = Limited Compatibility 

N = Not Compatible 

– = No Data Available 

Filter Membrane Chemical Compatibility Chart 

Filter Membrane Chemical Compatibility Chart 

Chemical 

Acids 

Acetic, 25% L C C C 

Acetic, Glacial L C C C 

Formic, 25% N C C C 

Hydrochloric, 25% L C C C 

Hydrochloric, Concentrated N N C C 

Sulfuric, 25% N C C C 

Sulfuric, Concentrated N C L L 

Nitric, 25% N C L C 

Nitric, Concentrated N C L N 

Phosphoric, 25% N C C C 

Trichloroacetic, 10% L C C C 

Bases 

Ammonium Hydroxide, 25% C C L — 

Sodium Hydroxide, 3 Normal C C C — 

Alcohols 

Amyl Alcohol C C C C 

Benzyl Alcohol C C C C 

Butyl Alcohol C C C C 

Ethanol, 70% C C C C 

Ethanol, 98% C C C C 

Ethylene Glycol C C C C 

Glycerine (Glycerol) C C C C 

Isopropanol C C C C 

Methanol, 98% C C C C 

n-Propanol C C C C 

Propylene Glycol C C C C 

Hydrocarbons 

Benzene C C C C 

Hexane, Xylene C C C C 

Kerosene, Gasoline C C C C 

Tetralin, Decalin C C C — 

Toluene C C C C 

Halogenated Hydrocarbons 

Carbon Tetrachloride C C C C 

Chlorobenzene (Mono) C C C C 

Chloroform L C C C 

Freon C C C C 

Methylene Chloride L C C C 

Trichloroethane C C C C 

Trichloroethylene C C C C 

Nylon 

PTFE 

PVDF 

Regenerated Cellulose 

Chemical 

Ketones 

Acetone C C N C 

Cyclohexanone C C N C 

Isopropylacetone C C N — 

Methyl Ethyl Ketone C C L C 

Methyl Isobutyl Ketone (MIBK) C C N C 

Esters 

2-Ethoxyethyl Acetate L L L C 

Amyl Acetate C C C C 

Benzyl Benzoate C C — C 

Butyl Acetate C C C C 

Ethyl Acetate C C C C 

Isopropyl Myristate C C — C 

Methyl Acetate L C L C 

Propyl Acetate L N C C 

Propylene Glycol Acetate L C — C 

Methyl Cellosolve Acetate C C C C 

Tricresyl Phosphate — C — C 

Oxides — Ethers 

Acetonitrile (Methyl Cyanide) C C C C 

Aniline L C C C 

Diethyl Acetamide C C C C 

Dimethyl Formamide C C N L 

Dimethyl Sulfoxide (DMSO) L C C L 

Dioxane C C L L 

Ethyl Ether C C C C 

Isopropyl Ether C C N — 

Pyridine C C L C 

Solvents with Nitrogen 

Tetrahydrofuran C C L L 

Triethanolamine C C C C 

Miscellaneous 

Formaldehyde Solution, 30% C C C L 

Hydrogen Peroxide, 30% N C C C 

Phenol, Aqueous, 10% N C L C 

Silicone Oil and Mineral Oil C C C C 

more info 

This is only a guide. Users should verify compatibility under actual 

use conditions. 

Nylon 

PTFE 

PVDF 


filtration 



Filtration Introduction 

Filter Device Selection 

Consider the number of samples, the sample volume, and 

the filtering mechanism you prefer to use. 

Syringe Filters 

Filtration is achieved by pushing the sample through the 

membrane with a syringe or other luer-connection device. 

Syringe filters allow you to control the rate of flow, which can 

be critical with delicate samples. Syringe filters also allow you 

to filter into nearly any tube, vial, or column that represents 

the next step in your analysis. 

Centrifuge Filter Tubes 

Filtration is achieved by pulling the sample through the 

membrane with centrifugal force. This allows you to 

simultaneously filter as many samples as your centrifuge 

can hold, and captures your filtrate in a clean microtube. 

6277 

Pre-Cut Membranes 

Multiple diameters offer a wide range of sample capacities, 

up to multiple liters. You need a filtration apparatus, such as 

a filter funnel or other vacuum-driven filtration device. 

Device Capacities 

Description 

Sample Volume 

Syringe Filter Capacities 

4mm Syringe Filter 

≤1mL 


≤5mL 


≤50mL 


≤50mL 


≤100mL 

Centrifuge Filter Tube Capacities 

2mL Tubes 20µL to 500µL 

50mL Tubes 

1mL to 25mL 

Pre-Cut Membrane Capacities 

13mm Pre-Cut Membranes 

up to 20mL 


up to 100mL 


multi-liter 

Membrane Selection 

filtration 

Choose a filter membrane based on the size and amount of particulate in the sample, the membrane’s chemical compatibility 

with the sample matrix, and potential interactions (binding) between the membrane and the sample components. This table offers 

general guidelines on membrane characteristics and applications. 

Membrane Selection Guide 

Membrane Type Features Common Uses Hydrophilic 

Solvent 

Resistance 

Protein 

Binding 

Nylon 

Good chemical compatibility and very General filtration 

Yes Good Medium 

low extractables 

sterilization, HPLC sample prep 

Polytetrafluoroethylene 

Compatible with strong acids and 

Gas, air, and solvent filtration No High* High 

(PTFE) 

aggressive solvents 

Polyvinylidene Fluoride 

Good flow rate characteristics. 

HPLC sample preparation and Yes High Low 

(PVDF) 

Ideal for chromatography applications. general filtration 


Yes High Very Low** 

*Highest solvent resistance. 

**Lowest protein binding. 

Universal membrane with excellent 

chemical resistance and low extractables. 

Use for aqueous and organic samples. 

HPLC sample prep 

general filtration 


Looking for mobile. 

inlet phase filters? 


6265 

5564 

318 




Nylon and PVDF Syringe Filters 

Nylon and PVDF syringe filters are available in a variety of 

diameters to address different sample sizes. 

Syringe Filter Specifications 

Diameter Max. Temp. Max. Pressure Hold-Up Volume 

Typical Sample 

Volume 

4mm 100°C 75psig Less than 15µL Up to 1mL 





6735 

Choose Nylon Membranes for General Filtration 

Needs 

Nylon is hydrophilic and has generally good solvent 

resistance. 

Choose PVDF Membranes for HPLC Sample 

Preparation 

PVDF is hydrophilic, highly solvent resistant, and low protein 

binding. 

Nylon Syringe Filters 

Diameter Pore Size Qty. Part No. 

4mm 0.2µm 100 2091 

4mm 0.45µm 100 2092 

13mm 0.2µm 100 2166 

13mm 0.45µm 100 2167 

17mm 0.2µm 100 62163 

17mm 0.45µm 100 62177 

25mm 0.2µm 100 2045 

25mm 0.45µm 100 2047 

30mm 0.2µm 100 62145 

30mm 0.45µm 100 62147 

PVDF Syringe Filters 


4mm 0.2µm 100 2227 

4mm 0.45µm 100 2228 

13mm 0.2µm 100 2647 

13mm 0.45µm 100 2648 

17mm 0.2µm 100 62130 

17mm 0.45µm 100 62209 

25mm 0.2µm 100 2223 

25mm 0.45µm 100 2224 

30mm 0.2µm 100 62193 

30mm 0.45µm 100 62195 



prefilters? 

See page 111. 


Need vials? 

We offer a full selection of 

vials, caps, and septa for 

all chromotography needs. 


filtration 


Need syringes? 

See pages 336–347 for 

our offering of high-quality 

Hamilton ® , VICI ® , and 

SGE ® syringes. 




PTFE and Regenerated Cellulose Syringe 

Filters 

PTFE and Regenerated Cellulose syringe filters are available 

in a variety of diameters to address different sample sizes. 

Syringe Filter Specifications 

Diameter Max. Temp. Max. Pressure Hold-Up Volume 

Typical Sample 

Volume 






Choose PTFE Membranes for Aggressive . 

Filtration Needs Such as Gas and . 

Solvent Filtration 

PTFE is hydrophobic with exceptional solvent resistance. 

PTFE Syringe Filters 


4mm 0.2µm 100 2394 

4mm 0.45µm 100 2395 

13mm 0.2µm 100 2164 

13mm 0.45µm 100 2165 

17mm 0.2µm 100 62118 

17mm 0.45µm 100 62136 

25mm 0.2µm 100 2089 

25mm 0.45µm 100 2090 

30mm 0.2µm 100 62189 

30mm 0.45µm 100 62191 

Choose Regenerated Cellulose Membranes 

for HPLC Sample Preparation and General 

Filtration Needs 

Regenerated Cellulose is hydrophilic with exceptional solvent 

resistance and very low protein binding. 

Regenerated Cellulose Syringe Filters 


4mm 0.2µm 100 656100 

4mm 0.45µm 100 656102 

17mm 0.2µm 100 62119 

17mm 0.45µm 100 62125 

30mm 0.2µm 100 62199 

30mm 0.45µm 100 62167 

filtration 


Need syringes? 

See pages 336–347 for 

our offering of high-quality 

Hamilton ® , VICI ® , and 

SGE ® syringes. 















320 



2mL Centrifuge Filter Tubes 

• For up to 850µL sample volume 

• Economical alternative to brand name filter tubes 

• Includes a glass support membrane 

Quickly and easily remove particulates and clarify small 

sample volumes. Less than 5µL hold-up volume provides 

near quantitative volumetric recovery. Maximum G-Force: 

10,000xG. 

50mL Centrifuge Filter Tubes 

• For up to 25mL sample volume 

• Use with fixed-angle rotor centrifuge 

• All tubes include a glass support membrane 

Alltech ® 50mL Centrifuge Filter Tubes remove particulates 

from larger sample sizes with any centrifuge that holds 

50mL conical tubes. They should only be used with a 

fixed-angle rotor centrifuge to prevent premature clogging 

of the membrane. Maximum operating temperature: 50°C. 

Maximum G-force: 2500xG. 

Alltech ® 2mL Filter Tubes 

Membrane Type, Pore Size Qty. Part No. 

Cellulose Acetate 

0.20µm 100 24126 

0.45µm 100 24133 

Nylon 

0.20µm 100 24137 

0.45µm 100 24139 

PVDF (Hydrophobic) 

0.20µm 100 24142 

0.45µm 100 24144 


0.20µm 100 24148 

0.45µm 100 24150 

4817 

Alltech ® 50mL Filter Tubes 

Membrane Type, Pore Size Qty. Part No. 

Cellulose Acetate 

0.20µm 50 24152 

0.45µm 50 24154 

Nylon 

0.20µm 50 24156 

0.45µm 50 24158 

PVDF (Hydrophobic) 

0.20µm 50 24160 

0.45µm 50 24162 

4784 

Forensic Spin Tubes 

• 600µL capacity 

• Extract biological fluids from cloth samples 

The 1.4mm mesh filter basket acts as a sieve to retain cloth 

samples and pass fluids to the receiver tube. The spin tube 

includes a 600µL filter basket and a 2mL microcentrifuge 

receiver tube with attached cap. 

Forensic Spin Tubes 


Forensic Spin 100 2566 

Forensic Spin 250 2567 

4818 

filtration 


321


Pre-Cut Filter Membranes 

• Variety of pore sizes and membrane types 

• Non-sterile 

Cellulose Acetate Pre-Cut Membranes 


13mm 0.20µm 100 2125 

13mm 0.45µm 100 2126 

25mm 0.20µm 100 2128 

25mm 0.45µm 100 2129 

47mm 0.20µm 100 2133 

47mm 0.45µm 100 2135 

Nylon Pre-Cut Membranes 


13mm 0.20µm 100 2046 

13mm 0.45µm 100 2044 

25mm 0.20µm 100 2050 

25mm 0.45µm 100 2048 

47mm 0.20µm 100 2034 

47mm 0.45µm 100 2024 

4777 

Cellulose Acetate Membranes 

Cellulose Acetate Membranes are hydrophilic and exhibit 

very low protein retention. Use for general biological filtration, 

sterilization, and filtration of aqueous solutions. 

Nylon Membranes 

Nylon Membranes are hydrophilic and resistant to many 

solvents. Use for filtration and clarification of aqueous or 

organic solutions. 

PTFE Membranes 

PTFE Membranes are hydrophobic and highly solvent 

resistant. Use for filtration of air, gases, and non-aqueous 

solutions. Pre-treat with methanol before using with 

aqueous solutions. 

PTFE Pre-Cut Membranes 


13mm 0.20µm 100 2013 

13mm 0.45µm 100 2015 

25mm 0.20µm 100 2023 

25mm 0.45µm 100 2029 

25mm 1.00µm 100 2056 

47mm 0.20µm 100 2057 

47mm 0.45µm 100 2058 

47mm 1.00µm 100 2059 

4777 

more info 

Need help selecting the correct 

membrane? 

See pages 317–318 for membrane 

specifications and suggested 

use/applications. 

filtration 


Looking for solvent filtration 

apparatus? 

See page 127. 

6266 



Recommended Reagents for GC Derivatization 

Chemically Modify Your Sample to Improve the Chromatography 

Recommended Reagents for GC Derivatization 

Compound Type of Derivative ECD* Possible Reagent Choices 

Alcohols Acetate Acetic Anhydride/Pyridine 

TMS 

Sil-Prep, BSTFA/TMCS 

t-Butyl Dimethyl Silyl Ether 

t-Butyl-DMCS/Imidazole, MTBSTFA 

Aldehydes Dimethyl Acetal DMF/DMA 

Amines TMS Sil-Prep 

Acetate 

Pyridine-Plus 

Amine (Primary and Secondary) Acetate Acetic Anhydride/Pyridine 

TMS 

MSHFBA 

Amino Acids TMS BSTFA 

HFB-Isobutyl Ester 

HFB-IBA Kit 

Trifluoroacetate X Trifluoroacetic Anhydride 

Bile Acids Acetate Pyridine-Plus 

Carbohydrates Acetate Acetic Anhydride/Pyridine 

TMS 

Power Sil-Prep 

Catecholamines Trifluorocetate X N-(Trifluoroacetyl)imidazole 

Drugs: 

Alkaloids Heptafluorobutyrate X Heptafluorobutyric Anhydride 

TMS 

MSTFA, Sil-Prep 

Antibiotics TMS Sil-Prep, Power Sil-Prep 

Anticonvulsants TMS MSTFA, BSA/TMCS 

Barbiturates N-Methyl Barb-Prep 

Dimethyl Ketal 

DMF/DMA 

Cannabinoids Trifluoroacetate X N-(Trifluoroacetyl)imidazole 

TMS 

BSTFA, MSTFA 

Estrogens Acetate Pyridine-Plus 

Fatty Acids: 

C9 and Longer Methyl Ester Methanolic HCl, Meth-Prep I 

Short Chain (Up to C8) TMS MSTFA 

Methyl Ester 

Methanolic HCl, Meth-Prep I 

Glycerides (mono and di) Acetate Acetic Anhydride/Pyridine 

Glycerides (mono, di, and tri) Methyl Ester Sodium Methoxide/Methanol, Meth-Prep II 

Glycolipids, Sphingolipids Methyl Ester Sodium Methoxide/Methanol, Meth-Prep II 

Hydroxyamines TMS BSTFA 

Hydroxy Groups (Primary/Secondary) Heptafluorobutyrate X Heptafluorobutylimidazole 

Trifluoroacetate X N-(Trifluoroacetyl)imidazole 

17-Ketosteroids TMS DMF-Sil-Prep 

Phenols Heptafluorobutyrate X Heptafluorobutyric Anhydride 

TMS 

BSTFA 

Methyl Esters 

DMF/DMA 

Polyols TMS Sil-Prep, Power Sil-Prep 

Steroids TMS Sil-Prep 

Acetate 

Pyridine-Plus 

Trifluoroacetate X Trifluoroacetic Anhydride 

*Derivatives suitable for use with Electron Capture Detector (ECD). 

tech tip 

When do you use derivatization? 

When you need to: 

1. Increase or decrease the volatility of the analytes to improve separation 

2. Improve peak symmetry by reducing interaction of sample and column 

3. Increase detector response (i.e., ECD) 

4. Enhance thermal stability 

standards and reagents 






323


Ready-To-Use Silylation Mixes 

Silylation replaces an active hydrogen atom (-OH, -NH2, 

-NHR, -SH) with a silyl group. Generally, it reduces the 

polarity of the compound and decreases possibility of 

hydrogen bonding. 

t-Butyldimethylchlorosilyl/Imidazole Reagents 

• Derivatives of alcohols are more stable than TMS ethers 

to hydrolysis 

t-Butyldimethylchlorosilyl/Imidazole 


t-BuDMCS/Imidazole 10 x 1mL 18028 

POWER Sil-Prep (TMSIM:BSA:TMCS) Reagents 

• Powerful reagent for sterically hindered groups 

POWER Sil-Prep 


POWER Sil-Prep 10 x 1mL 18012 

DMF Sil-Prep (HMDS:TMCS:DMF) Reagents 

• Prevents enolization of keto groups 

5421 

DMF Sil-Prep 


DMF Sil-Prep 10 x 1mL 18015 

Sil-Prep (HMDS:TMCS:pyridine) Reagents 

• General purpose reagent for making TMS derivatives 

Sil-Prep 


Sil-Prep 10 x 1mL 18013 


tech tip 

Silylation Reagent Strength 

HMDS 

TMCS 

Sil-Prep 

MSTFA 

MSHFBA 

BSA 

BSTFA 

TMSIM 

BSTFA +1% TMCS 

POWER Sil-Prep 

tech tip 

What are the requirements for a good reagent? 

It will: 

1. Not cause any rearrangements or structural changes in 

the analyte 

2. Produce a reaction that is 95–100% complete 

3. Produce a stable derivative 

4. Not contribute to loss of sample during the reaction 

5. Produce a derivative that is inert to the column and connections 

6. Not produce interfering byproducts 


Looking for a Safe and Easy 

Way to Open Ampules? 

Try the Ampule Cracker. 

See page 380. 

4958 



Individual Silylation Reagents 

BSA [N,O-bis(trimethylsilyl)acetamide] 

• Powerful silylating reagent and solvent for polar 

compounds 

BSA 


BSA 10mL 18056 

BSA 25mL 18057 

BSA 10 x 1mL 18034 

BSTFA (N,O-bis(trimethylsilyl)trifluoroacetamide) 

• More volatile reagent and byproducts compared to BSA 

• Powerful silylating reagent; even more effective combined 

with TMCS as a catalyst 

BSTFA 


BSTFA 10mL 18085 

BSTFA 10 x 1mL 18040 

BSTFA + 1% TMCS 10mL 18087 

BSTFA + 1% TMCS 10 x 1mL 18089 

CMDMCS 

• Longer retention times than TMCS derivatives 

• Enhanced ECD response 

CMDMCS 


CMDMCS 5mL 18031 

MSTFA 

(N-Methyl-N-trimethylsilyltrifluoroacetamide) 

• Ideal reagent for drug analysis because excess reagent 

and byproducts elute during solvent delay 

• May be used directly on HCl salts of compounds 

MSTFA 


MSTFA 10mL 18061 

MSTFA 1 x 1mL 18038 

MTBSTFA [N-(tert-butyldimethylsilyl)- 

N-methyltrifluoroacetamide] 

• Derivatives more stable than common TMS derivatives 

• Produces a distinct M-57 ion in GC/MS analysis 

• Even more effective when combined with TBDMCS as 

a catalyst 

MTBSTFA 


MTBSTFA 10 x 1mL 18097 

MTBSTFA 10mL 18102 

MTBSTFA + 1% TBDMCS 10 x 1mL 18155 

MTBSTFA + 1% TBDMCS 10mL 18148 

TMCS (Trimethylchlorosilane) 

• Similar to HMDS when used alone 

• Very effective as a catalyst to other reagents 

DMCS (Dimethylchlorosilane) 

• DMS derivatives are more sensitive to hydrolysis than 

TMS derivatives 

DMCS 


DMCS 3 x 10mL 18071 

HMDS (Hexamethyldisilazane) 

• Slow and inefficient used alone 

• Very effective in combination with TMCS 

TMCS 


TMCS 3 x 10mL 18091 

TMCS 10 x 1mL 18084 

TMSIM (N-Trimethylsilylimidazole) 

• Preferentially silylates hydroxyl groups 

• Best silylation reagent when water is present 

TMSIM 


TMSIM 10 x 1mL 18050 


HMDS 


HMDS 25mL 18069 

HMDS 10 x 1mL 18003 

MSHFBA (N-Methyl- 

N-trimethylsilylheptafluorobutyramide) 

• Similar to MSTFA but does not produce harmful deposits 

in FID 

MSHFBA 


MSHFBA 10mL 214610 

MSHFBA 20 x 1mL 2146201 

Glass Conditioning Reagent 

Glass-Prep (5% DMDCS in toluene) 

• Deactivate any glass surface 

Glass-Prep 


Glass-Prep 100mL 9700 

Glass-Prep 400mL 2233 

DMDCS, Neat 10 x 5mL 18090 


325


Alkylation (Esterification) 

Reagents 

Alkylation replaces an acidic hydrogen (carboxylic acids, 

phenols) with an alkyl group. Alkyl esters are extremely stable 

and can be stored for long periods of time. 

Meth-Prep I 

• On-column derivatization 

• Aqueous solution does not give solvent peak 

Meth-Prep I is a 0.2N aqueous solution of 

(m-trifluoromethylphenyl) trimethylammonium hydroxide. 

When the reagent is mixed with fatty acids and then 

injected into a GC injector at 240ºC, the methyl esters 

are formed along with the by-product, m-trifluoromethylphenyl 

dimethylamine. The reaction is clean, fast, and quantitative. 

Meth-Prep I, being in aqueous solution, does not give a 

solvent peak. This may be advantageous in some cases. 

Meth-Prep I 


Meth-Prep I 10 x 1mL 18005 

Transesterification Reagents 

Transesterification is the process of converting one type of 

ester into another. Most commonly this is a very large ester 

(i.e., triglycerides, steryl esters, wax esters, phospholipids) 

converted into a methyl ester that is more easily analyzed. 

Meth-Prep II 

• In one step forms fatty acid methyl esters from 

triglycerides at room temperature 

• Requires no extraction before injecting into GC 

• Faster than sodium methoxide reagents 

Meth-Prep II is a 0.2N methanolic solution of 

m-trifluoromethylphenyl trimethylammonium hydroxide. 

This one-step reagent simplifies the transesterification 

of triglycerides to methyl esters. It is faster than sodium 

methoxide and the reaction occurs at room temperature. 

This quantitative reaction requires no extractions or additional 

treatment prior to gas chromatographic analysis. 

Meth-Prep II 


Meth-Prep II 10 x 1mL 18007 


Instant Methanolic HCl Reagent Kit 

• Acid catalyzed esterification of alcohols 

• Reagent is generated before use and is stable for one 

week at room temperature 

Instant Methanolic HCl Reagent Kit 


Instant Methanolic HCl Reagent Kit ea 18053 

Anhydrous Acetyl Chloride 5 x 2.8mL 18095 

Anhydrous Methanol 10 x 5mL 18157 

4801 


Looking for syringes to use 

with reagents? 

See page 337–342. 

Sodium Methoxide/Methanol 

• Converts a wide range of large esters to fatty acid 

methyl esters 

Sodium Methoxide/Methanol 


Sodium Methoxide/Methanol 10 x 5mL 18018 

Sodium Methoxide/Methanol 25mL 218025 


Looking for amino acid derivatization kits? 

See page 63. 







Acylation 

Acylation reduces the polarity of amino, hydroxyl and thiol 

groups on multi-functional molecules such as carbohydrates 

and amino acids. 

Acetic Anhydride/Pyridine 

• Used for acetylation of alcoholic and phenolic hydroxyl 

groups; and primary and secondary amino groups 

Acetic Anhydride/Pyridine 


Acetic Anhydride/Pyridine Kit ea 18100 

Acetic Anhydride 10 x 1mL 18103 

Pyridine-Plus 

• Much more powerful than acetic anhydride/pyridine 

• Rapid reaction and fewer byproducts 

Pyridine-Plus 


Pyridine-Plus Kit ea 18105 

Fluorinated Imidazoles 

• No acidic byproducts compared to anhydride reagents 

Fluorinated Imidazoles 


TFAI (N-Trifluoroacetylimidazole) 10 x 0.2g 18046 

HFBI (N-Heptafluorobutyrylimidazole) 10 x 0.2g 18048 

Perfluoroalkyl Anhydrides 

• Produces derivatives suitable to electron capture 

detection 

Perfluoroalkyl Anhydrides 


Trifluoroacetic Anhydride 5 x 1mL 18083 

Pentafluoropropionic Anhydride 25g 65192 

Pentafluoropropionic Anhydride 5 x 1mL 18116 

Heptafluorobutyric Anhydride 25g 63163 

Heptafluorobutyric Anhydride 5 x 1mL 18118 


Looking for reaction vials to 

use with reagents? 

See page 375. 

Specialty Reagents 

Amino Acid Derivatization Kits 

• Simple two-step procedure 

• Three different reagents available: TFA, PFP, and HFB 

Amino Acid Derivatization Kits 


TFA-IPA Derivatization Kit ea 18092 

PFP-IPA Derivatization Kit ea 18093 

HFB-IPA Derivatization Kit ea 18094 

Barb-Prep 

• On-column methylation of barbiturates 

• No byproduct formation 

Barb-Prep 


Barb-Prep 10 x 1mL 18009 

DMF-DMA 

• Novel reagent for derivatization of fatty acids, amino 

acids, amines, and barbiturates 

DMF-DMA 


DMF-DMA 10 x 1mL 18051 

LC Reagents 

UV Enhancing 

UV Enhancing 


p-Bromophenacyl Derivatization Kit — 18036 

p-Nitrobenzyloxyamine HCl (PNBA) 1g 510113 

Fluorescence Enhancing 

Fluorescence Enhancing 


o-Phthalaldehyde (OPA) 5g 35606 


4758 


327


IPC Reagents 

• Comparable to Waters ® PIC ® Reagents 

• Preformulated at optimum concentration and pH 

• Convenient—Simply dilute with HPLC grade solvents 

“A” Series 

• Used for chromatography of acidic compounds 

“B” Series 

• Used for chromatography of basic compounds 

A Series and B Series 


IPC A, Tetrabutylammonium Phosphate 5 x 15mL 185101 

IPC B5, Sodium Pentanesulfonate 5 x 25mL 185110 

IPC B7, Sodium Heptanesulfonate 5 x 25mL 185103 

“Low UV”* Series 

• Suitable for use at wavelengths down to 200nm 

Low UV Series 


Low UV IPC A, Tetrabutylammonium Sulfate 5 x 20mL 185149 

Low UV IPC B5, Tetrabutylammonium Phosphate 5 x 20mL 184198 




*For use at wavelengths below 240nm. 

Table 1—IPC Reagents 


IPC A 

IPC B5 

IPC B6 

IPC B7 

IPC B8 

Low UV IPC A 

Low UV IPC B5 

Low UV IPC B6 

Low UV IPC B7 

Low UV IPC B8 

1 2 

3 

IPC B7 Reagent 

4 

Waters 

PIC ® A 

PIC ® B5 

PIC ® B6 

PIC ® B7 

PIC ® B8 

Low UV PIC ® A 

Low UV PIC ® B5 




5870 


2. Riboflavin 

3. Pyridoxine 

4. Thiamine 

Ion-Pair Salts 

Grace offers high-purity ion-pairing salts for those wishing to 

formulate their own mobile phases. 


• High purity for HPLC applications 

Ion-Pair Salts 


1-Pentanesulfonate 25g 403125 

1-Hexanesulfonate 25g 403126 

1-Heptanesulfonate 25g 403127 

1-Octanesulfonate 25g 403128 

Tetrabutylammonium Phosphate, 0.5M, pH 7.5) 10mL 680502 

0 2 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

4 

6 

8 Min. 

Adsorbosphere HS C18, 7µm, 

4.6 x 250mm HPLC Column 

Methanol:Water (50:50) both with IPC B7 

1.0mL/min 

UV at 254nm 


Looking for mobile phase 

storage bottles? 

See page 125–127. 

5595 



Gas Standards 

• Portable, lightweight, and easy-to-use disposable cylinders 

• Each standard comes with a certificate of analysis 

MicroMAT-10 

10L Disposable Cylinder 

6346 6347 6675 

MicroMAT-14 


MicroMAT-58 


Single Component Gas Standards 

Single Component Mixtures 

Analyte/Concentration Balance Cylinder Size Part No. 

Acetylene 

1% Helium MicroMAT-10 G0413 

1% Nitrogen MicroMAT-10 G0423 

Argon 

99.995% — MicroMAT-14 M7003 

Carbon Dioxide 

99.80% — MicroMAT-14 M7006 

100ppm Helium MicroMAT-10 G1211 




Carbon Monoxide 

1000ppm Helium MicroMAT-10 24047 

10% Helium MicroMAT-10 24054 

Ethane 

99.00% — MicroMAT-14 M7012 

Ethylene 


100ppm Helium MicroMAT-14 M7030 


1000ppm Nitrogen MicroMAT-14 24022 

8-10ppm Air MicroMAT-14 24096 

Helium 

99.995% — MicroMAT-14 M7005 

Hydrogen 

99.99% — MicroMAT-14 M7004 

100ppm Nitrogen MicroMAT-14 M7125 

1000ppm Nitrogen MicroMAT-10 G1522 


Nitrogen 

99.998% — MicroMAT-14 M7002 

Single Component Mixtures 

Analyte/Concentration Balance Cylinder Size Part No. 

Nitrous Oxide 

98.00% — MicroMAT-14 M7009 

0.1ppm Nitrogen MicroMAT-14 24042 

1.0ppm Nitrogen MicroMAT-14 24044 



Methane 

99.00% — MicroMAT-14 M7011 




10% Helium MicroMAT-14 24066 

100ppm Nitrogen MicroMAT-14 M7029 


10% Nitrogen MicroMAT-14 24067 

10ppm Air MicroMAT-14 19781 

Oxygen 

99.5% — MicroMAT-14 M7001 

100ppm Helium MicroMAT-14 M7121 






Propane 

99.00% — MicroMAT-14 M7014 

Vinyl Chloride (NIST Traceable) 





329


Multi-Component Gas Mixtures 


Multi-Component Gas Mixtures 

Description Concentration Cylinder Size Part No. 

BTEX in Nitrogen 

Benzene, Ethylbenzene, Toluene, Xylene 1ppm (mix) MicroMAT-58 24076 

10ppm (mix) MicroMAT-58 24099 

100ppm (mix) MicroMAT-58 24115 

C2 to C4 Alkyne in Nitrogen 

Acetylene, 1-Butyne, 2-Butyne, Propyne 15ppm each MicroMAT-10 19797 

C1 to C4 Hydrocarbons in Nitrogen 

Methane, Ethane, Ethylene, Acetylene, Propane, Propylene, Propyne, 

15ppm each MicroMAT-14 24130 

n-Butane 

C4 Hydrocarbons in Nitrogen 

n-Butane, Isobutane, 2-Butene (cis and trans), 1-Butene, Isobutylene, 


1,3-Butadiene, Ethyl Acetylene 

2-Olefin Mixture in Nitrogen 

2-Butene (cis and trans), 2-Pentene (cis and trans), 2-Hexene (cis 


and trans) 

C2 to C6 Olefin Mixture in Helium 

Ethylene, Propylene, 1-Butene, 1-Pentene, 1-Hexene 100ppm each MicroMAT-14 19784 

1000ppm each MicroMAT-14 M7020 

C2 to C6 Olefin Mixture in Nitrogen 

Ethylene, Propylene, 1-Butene, 1-Pentene, 1-Hexene 1000ppm each MicroMAT-14 M7019 

C1 to C6 n-Paraffin Mixture in Helium 

Methane, Ethane, Propane, Butane, Pentane, Hexane 100ppm each MicroMAT-14 19782 


C1 to C6 n-Paraffin Mixture in Nitrogen 

Methane, Ethane, Propane, Butane, Pentane, Hexane 15ppm each MicroMAT-14 19772 



Branched Paraffin Mixture in Nitrogen 

Isobutane, 2-Methylbutane, 2,2-Dimethylpropane, 2-Methylpentane, 


3-Methylpentane, 2,2-Dimethylbutane 

CO and CO2 in Nitrogen 

Carbon Monoxide, Carbon Dioxide 25ppm (CO), 1000ppm (CO2) MicroMAT-14 24136 

CO, CO2, Methane, Ethane, Ethylene, Acetylene in Nitrogen 

1% each MicroMAT-14 M7035 

CO, CO2, Methane, H2, O2 in Nitrogen 

1% each MicroMAT-14 M7036 

CO, CO2, CH4, H2, O2, N2 in Helium 

Carbon Monoxide, Carbon Dioxide, Methane, Hydrogen, Oxygen, Nitrogen 

5% (CO), 5% (CO2), 4% (CH4), 4% (H2), 

5% (O2), 5% (N2) 

MicroMAT-14 19792 

CO, CO2, O2 in Nitrogen 

Carbon Monoxide, Carbon Dioxide, Oxygen 7% (CO), 15% (CO2), 3% (O2) MicroMAT-14 M7041 

CO, CO2, CH4, O2 in Nitrogen 

Carbon Monoxide Carbon Dioxide, Methane, Oxygen 7% (CO), 15% (CO2), 4.5% (CH4), 7% (O2) MicroMAT-14 19791 

CO, CO2, H2, O2 in Nitrogen 

0.5% each MicroMAT-14 24138 

0.5% each (CO, CO2, O2), 5% H2 MicroMAT-14 M7037 

CO2, O2 in Nitrogen 

1% each MicroMAT-14 24029 

1% (CO2), 2% (O2) MicroMAT-14 M7040 

2% (CO2), 24% (O2) MicroMAT-14 24034 



Special Gas Standards 

Reactive Gas Standards 

Reactive Gas Standards 


Hydrogen Sulfide in 10ppm MicroMAT-58 24102 

Nitrogen 

95ppm MicroMAT-58 24105 

Nitric Oxide in Nitrogen 20-30ppm MicroMAT-58 24107 

Hardware for Using Gas 

Cylinders 

MicroMAT-10 Syringe Adapter 

• Maximum pressure 120psig 

Natural Gas Standards 

• Certificate of analysis 

• Shelf life one year 

Natural Gas Standards Concentrations 

GPA Calorimetric High Ethane Helium-Enriched 

Helium 0.50 — — 2.00 

Nitrogen 5.00 2.50 9.00 1.60 

Carbon Dioxide 1.00 3.00 0.50 0.20 

Ethane 9.00 3.50 12.50 3.00 

Propane 6.00 1.00 7.00 1.70 

Isobutane 3.00 0.40 3.00 1.00 

n-Butane 3.00 0.40 3.00 1.00 

Isopentane 1.00 0.15 0.50 0.30 

n-Pentane 1.00 0.15 0.50 0.30 

Neopentane — 0.10 — — 

n-Hexane — 0.05 — — 

n-Heptane — 0.02 — — 

Methane Balance Balance Balance Balance 


Description Cylinder Size Part No. 

Aerosol Syringe Adapter MicroMAT-10 8048 

Replacement Septa, 12/pk — 75801 


• Maximum pressure 240psig 

• May be used with any 

1/8" NPT (F) outlet 

MicroMAT-10 Syringe 

Adapter 

5386 

Natural Gas Standards 


Natural Gas GPA 

See above MicroMAT-14 24100 

Standard 

Natural Gas Calorimetric See above MicroMAT-14 24116 

Standard 

Natural Gas High Ethane See above MicroMAT-14 24128 

Standard 

Natural Gas Helium- 

Enriched Standard 

See above MicroMAT-14 24131 


Looking for Gastight syringes? 


5156 

MicroMAT-58 Regulator 

• Stainless steel with hose 

barb outlet 

• Push button on/off control 

• Preset 0.3SLPM flow rate 

MicroMAT-14 Syringe 

Adapter 



Syringe Adapter MicroMAT-14 8810 

Replacement Septa, 10/pk — 8812 

5385 




0–30psig, C-10 Valve MicroMAT-58 37201 

6674 



331


Fatty Acid and Methyl Ester Standards 

• Quality certificate supplied with every standard and mix 

Fatty Acid and Methyl Ester Standards 



Carbon # Description Qty. Part No. Qty. Part No. 

11:0 Henedecanoic Acid (Undecylic) 0.5g 622110 0.5g 623110 

12:0 Dodecanoic Acid (Lauric) 1g 622120 1g 623120 

13:0 Tridecanoic Acid (Tridecylic) 1g 622130 1g 623130 

14:0 Tetradecanoic Acid (Myristic) 1g 623140 1g 623140 

15:0 Pentadecanoic Acid (Pentadecylic) 0.5g 622150 0.5g 623150 

16:0 Hexadecanoic Acid (Palmitic) 1g 622160 1g 623160 

16:1 cis-9-Hexadecenoic Acid (Palmitoleic) 0.1g 6221610 0.1g 6231610 

17:0 Heptadecanoic Acid (Margaric) 1g 622170 1g 623170 

18:0 Octadecanoic Acid (Stearic) 1g 622180 1g 623180 

18:1 cis-9-Octadecenoic Acid (Oleic) 1g 6221810 1g 6231810 

18:1 trans-9-Octadecenoic Acid (Elaidic) 1g 6221811 0.5g 6231811 

18:1 cis-11-Octadecenoic Acid (cis-Vaccenic) — — 0.1g 62318101 

18:1 trans-11-Octadecenoic Acid (trans-Vaccenic) — — 0.1g 62318121 

18:2 cis, cis-9,12-Octadecadienoic Acid (Linoleic) 1g 6221820 1g 6231820 

18:2 trans,trans-9,12-Octadecadienoic Acid (Linoelaidic) 0.1g 6221821 0.1g 6231821 

18:3 All cis-9,12,15-Octadecatrienoic Acid (Linolenic) 0.1g 62218300 0.1g 6231830 

18:3 All cis-6,9,12-Octadecatrienoic Acid (γ-Linolenic) 0.1g 62218301 0.1g 6231831 

20:0 Eicosanoic Acid (Arachidic) 1g 6220200 1g 623200 

20:1 cis-11-Eicosenoic Acid 0.1g 6220201 0.1g 623201 

20:4 All cis-5,8,11,14-Eicosatetraenoic Acid (Arachidonic) 0.1g 6220204 0.1g 623204 

20:5 All cis-5,8,11,14,17-Eicosapentaenoic Acid 10mg 6220205 10mg 623205 

22:0 Docosanoic Acid (Behenic) 1g 6220220 1g 623220 

22:5 All cis-7,10,13,16,19-Docosapentaenoic Acid 10mg 6220225 10mg 6232250 

23:0 Tricosanoic Acid 0.1g 6220230 0.1g 623230 

24:0 Tetracosanoic Acid (Lignoceric) 0.1g 6220240 0.1g 623240 


Fatty Acid Methyl Ester Mixes 

Description Components Qty. Part No. 

K101 C8:0, C10:0, C12:0, C14:0, C16:0, C18:0, C20:0 100mg 625001 

K103 C12:0, C14:0, C14:1, C16:0, C16:1 cis 50mg 625002 

K1000 C13:0, C15:0, C17:0, C19:0, C21:0 50mg 625003 

K104 C14:0, C16:0, C18:0, C20:0, C22:0, C24:0 100mg 625004 

K110 C16:0, C16:1 cis, C16:1 trans, C18:0, C18:1 cis-9, C18:1 trans-9, C18:2 cis,cis, C18:2 trans,trans 100mg 625005 

K108 C16:0, C18:0, C18:1 cis-9, C18:2 cis,cis, C18:3 all cis 50mg 625006 

K2000 C18:0, C18:1 cis-9, C18:2 cis,cis, C18:3 all cis, C20:4 all cis 50mg 625007 

K3000 C18:2 cis,cis, C18:3 all cis, C20:4 all cis, C22:6 all cis 50mg 625008 

K4000 C20:0, C20:1 cis, C20:2 cis,cis, C20:3 all cis, C20:4 all cis 50mg 625009 

K107 C20:0, C20:1 cis, C22:0, C22:1 cis, C24:0, C24:1 cis 50mg 625010 

K5000 Mix A C6:0, C8:0, C10:0, C12:0 100mg in 5mL 625017 

K5000 Mix B C12:0, C14:0, C16:0, C18:0 100mg in 5mL 625018 

L207 C18:0, C18:1 cis-9, C20:0, C22:0, C22:1 cis, C24:0, C24:1 cis 50mg 625021 

L205 C18:0, C18:1 cis-9, C18:2 cis,cis, C18:3 all cis, C20:4 all cis 100mg 625022 

L209 C20:0, C20:1 cis, C20:4 all cis, C22:1 cis, C22:6 all cis 25mg 625023 

AOCS Mix 1 C16:0, C18:0, C18:1 cis-9, C18:2 cis,cis, C18:3 all cis, C20:0 50mg 625024 

AOCS Mix 3 C16:0, C18:0, C18:1 cis-9, C18:2 cis,cis, C18:3 all cis, C20:0, C22:0, C22:1 cis, C24:0 50mg 625026 

AOCS Mix 3A C16:0, C18:0, C18:1 cis-9, C18:2 cis,cis, C18:3 all cis, C20:0, C20:1 cis, C22:0, C22:1 cis, C24:0 25mg 625027 

AOCS Mix 5 C8:0, C10:0, C12:0, C14:0, C16:0, C18:0, C18:1 cis-9, C18:2 cis,cis 50mg 625029 

AOCS Mix 6 C14:0, C16:0, C16:1 cis, C18:0, C18:1 cis-9, C18:2 cis,cis, C18:3 all cis 50mg 625030 



Hydrocarbons and Miscellaneous Standards and Kits 

• Quality certificate supplied with every standard and mix 

Use Standards and Mixes with complete confidence in purity 

and quality. Don’t waste your valuable lab time to verify the 

standards you need. To satisfy your documentation needs, 

Grace supplies a purity certificate for every standard and mix. 



Oxalic Acid, 1000ppm 125mL 37033 

Maleic Acid, 1000ppm 125mL 37037 

Malic Acid, 1000ppm 125mL 37039 

Succinic Acid, 1000ppm 125mL 37043 

Formic Acid, 1000ppm 125mL 37045 

Acetic Acid, 1000ppm 125mL 37047 

Citric Acid, 1000ppm 125mL 37049 

Tartaric Acid, 1000ppm 125mL 37051 

Lactic Acid, 1000ppm 125mL 37053 

Abietic Acid, 1000ppm 125mL 37055 

Methane Sulfonic Acid, 1000ppm 125mL 37057 

n-Paraffin Mixtures 


Neat Mixtures (No Solvent— Equal Weights of Components) 

C5, C6, C7, C8 2mL 628001 

C7, C8, C9, C10 2mL 628002 

C9, C10, C11, C12 2mL 628003 

C11, C12, C13, C14 2mL 628004 

C12, C14, C16, C18 2mL 628005 

Mixtures (In 5mL Hexane — Equal Weight of Components) 

C16, C18, C20, C22 0.5g 628006 

C20, C22, C24, C26 0.1g 628007 

C24, C26, C28, C30 50mg 628008 

C28, C30, C32, C34 50mg 628009 

C32, C34, C36, C38 50mg 628010 

Hydrocarbons (n-Paraffins) 

Carbon No. Description Qty. Part No. 

C5 n-Pentane 1g 620050 

C6 n-Hexane 1g 620060 

C7 n-Heptane 1g 620070 

C8 n-Octane 1g 620080 

C9 n-Nonane 1g 620090 

C10 n-Decane 1g 620100 

C11 n-Hendecane 1g 620110 

C12 n-Dodecane 1g 620120 

C13 n-Tridecane 1g 620130 

C14 n-Tetradecane 1g 620140 

C15 n-Pentadecane 1g 620150 

C16 n-Hexadecane 1g 620160 

C17 n-Heptadecane 1g 620170 

C18 n-Octadecane 1g 620180 

C19 n-Nonadecane 1g 620190 

C20 n-Eicosane 1g 620200 

C21 n-Heneicosane 1g 620210 

C22 n-Docosane 1g 620220 

C23 n-Tricosane 1g 620230 

C24 n-Tetracosane 1g 620240 

C25 n-Pentacosane 0.1g 620250 

C26 n-Hexaconsane 0.1g 620260 

C27 n-Heptacosane 0.1g 620270 

C28 n-Octacosane 1g 620280 

C29 n-Nonacosane 0.1g 620290 

C30 n-Triacontane 0.1g 620300 

C32 n-Dotriacontane 0.1g 620320 

C34 n-Tetratriacontane 0.1g 620340 

C36 n-Hexatriacontane 0.1g 620360 

C38 n-Octatriacontane 0.1g 620380 

C40 n-Tetracontane 0.1g 620400 

C44 n-Tetratetracontane 0.1g 620440 

Aromatic Hydrocarbon Mix 


BETX Mix 2g 629001 

Contains equal amounts of benzene, toluene, ethylbenzene, o-xylene, m-xylene, and 

p-xylene. 

Standards Kits 

Description Kit Contains 100mg Each Component Part No. 

Free Fatty Acid Kits 

Short Chain C2, C3, C4, C5, C6, C7, C8 18600 

Even Carbon C10, C12, C14, C16, C18, C20, C22 186021 

Odd Carbon C9, C11, C13, C15, C17, C19, C21, C23 18608 

Fatty Acid Ester Kits 

Methyl Esters, Even Carbon C10, C12, C14, C16, C18, C20, C22 18603 

Methyl Esters, Odd Carbon C9, C11, C13, C15, C17, C19, C21 18609 

Ethyl Esters, Even Carbon C6, C8, C10, C12, C14, C16, C18, C20, C22, C24 18610 

Ethyl Esters, Odd Carbon C7, C9, C11, C13, C15, C17, C19, C21, C23 18611 

Hydrocarbon Kits 

Saturated, Even Carbon C10, C12, C14, C16, C18, C20, C22, C24, C28, C32, C36 18484 

Saturated, Odd Carbon C9, C11, C13, C15, C17, C19, C21, C23, C25 18485 



333


Alltech ® IC Standards 

• High purity for sensitive applications 

• Prepared from NIST standard reference materials 

• Certified by two independent methods 

• Detailed Certificate of Analysis included 

Certified Organic Anion Standards 

Organic Anion Standards (125mL) 

1000ppm 

Description 

Part No. 

Acetate 37052 

Citrate 37091 

Formate 37050 

Glycolate 37054 

Lactate 37093 

Malate 37095 

Maleate 37099 

Methanesulfonate 37221 

Nitrilotriacetate (NTA) 37228 

Oxalate 37056 

Propionate 37229 

Succinate 37223 

Tartrate 37224 

3493 

Certified Organic Acid Standards 


Certified Anion and Cation Standards 

Anion Standards (125mL) 

1000ppm 

Part No. 

200ppm 

Part No. 

Description 

Bromate 37058 — 

Bromide 37005 37006 

Chlorate 37034 — 

Chloride 37009 37010 

Chlorite 37038 — 

Chromate 37042 — 

Fluoride 37011 37012 

Iodide 37013 — 

Nitrate 37019 37020 

Nitrate-N 37234 — 

Nitrite 37021 37022 

Nitrite-N 37235 — 

Perchlorate 37048 — 

Phosphate 37023 37024 

Phosphate-P 37236 — 

Sulfate 37031 37032 

Thiocyanate 37046 — 

Cation Standards (125mL) 

1000ppm 

Part No. 

200ppm 

Part No. 

Description 

Ammonium 37001 37002 

Ammonium-N 37233 — 

Calcium 37007 37008 

Lithium 37015 37016 

Magnesium 37017 37018 

Potassium 37025 37026 

Sodium 37029 37030 

Organic Acid Standards (125mL) 

1000ppm 

Description 

Part No. 

Oxalic Acid 37033 

Maleic Acid 37037 

Malic Acid 37039 

Succinic Acid 37043 

Formic Acid 37045 

Acetic Acid 37047 

Citric Acid 37049 

Tartaric Acid 37051 

Lactic Acid 37053 

Abietic Acid 37055 

Methanesulfonic Acid 37057 

Certified Amine Standards 

Amine Standards (125mL) 

1000ppm 

Description 

Part No. 

Ethanolamine 37225 

Diethanolamine 37226 

Triethanolamine 37227 

Monomethylamine 37230 

Dimethylamine 37231 

Trimethylamine 37232 



Alltech ® IC Standards 

Certified Multi-Standard Kits 

• Cost-effective ways to purchase multiple single-ion 

standards 

Multi-Standard Kits 

Description 

Anion Kits 

200ppm Kit: Contains 125mL ea of 200ppm 

Certified Cl, F, Br, NO3, NO2, PO4, and SO4 


Certified Cl, F, Br, NO3, NO2, PO4, and SO4 

Anion and Oxyhalide Kit: (EPA 300.1Part B) Contains 

125mL ea of 200ppm certified Cl, F, Br, NO3, NO2, PO4, 

and SO4, ClO2, ClO3, BrO3 

Cation Kits 


Certified Li, Na, NH4, K, Mg, and Ca 


Certified Li, Na, NH4, K, Mg, and Ca 

best 

value 

Part No. 

37035 

37036 

37085 

37040 

37041 

EZ-LUTE Buffer 

Concentrates 

• Simplify mobile phase preparation 

• For anion, cation, and organic acid analyses 

• Dilution instructions included 

Certified Multi-Component Mixtures 

• Multiple ions in a single mixture 

Multi-Component Mixtures (125mL) 

Description 

Anion Mixtures 

Mix A: 125mL Mixture Contains: 

F(10ppm), Cl(20ppm), O2(20ppm), Br(20ppm), 

NO3(20ppm), PO4(30ppm), SO4(30ppm) 

Mix 1: 125mL Mixture Contains: 

F(1ppm), Cl(5ppm), NO2(5ppm), Br(5ppm), NO3(5ppm), 

PO4(5ppm), SO4(5ppm) 


F(1ppm), Cl(10ppm), NO2(10ppm), Br(10ppm), 












Cl(1000ppm), Br(1000ppm), NO3(1000ppm), 

PO4(1000ppm), SO4(1000ppm) 


Cl(15ppm), Br(15ppm), NO3(15ppm), 

PO4(15ppm), SO4(5ppm) 


F(25ppm), Cl(50ppm), SO4(100ppm) 

Cation Mixtures 

Mix A: 125mL Mixture Contains: 

Li(0.5ppm), Na(3ppm), NH4(3ppm), K(6ppm) 

Mix B: 125mL Mixture Contains: 

Li(0.2ppm), Na(1.5ppm), NH4(1.5ppm), K(2.5ppm), 

Mg(2.0ppm), Ca(2.0ppm) 

Part No. 

26910200 

269106 

269107 

269108 

269109 

269110 

269111 

269112 

269113 

26910300 

26910400 

EZ-LUTE Buffers for accurate concentrations every time. 

EZ-LUTE Buffer Concentrates* 

Description 

Part No. 

Non-suppressed Buffers, 12 x 25mL 

4mM Phthalic Acid 470217 

4mM Phthalic Acid, pH 4.5 470216 

4mM p-Hydroxybenzoic Acid 470212 



5mM LiOH/Benzoate 470213 

3mM Methanesulfonic Acid 470211 

Suppressor-based Buffers, 1 x 100mL 

1.7mM Bicarbonate/1.8mM Carbonate 470119 





3.6mM Carbonate 470123 

500mM Bicarbonate 470209 

500mM Carbonate 470210 

*Concentration after dilution. 

more info 

Custom Standards Available 

If you need it, we can make it, whether a single ion or a complex 

mixture. Request a quote for part number C2690, in any volume 

from 125mL to 1-liter. Fast delivery! 

6561 



335


Syringe Selection Guide 

Syringe Selection Guide 

Manufacturer Type Description Page # 

Hamilton ® Syringes 

Series 600 General purpose, 2.5µL to 5µL 338 

Series 700 General purpose, 5µL to 500µL 338 

Series 900 Economical syringe with reinforced plunger, 5µL to 10µL 338 

Series 800 General purpose with reinforced plunger, 5µL to 250µL 339 

Series 1000 Gas and liquid tight, 1mL to 100mL 339 

Series 7000 Plunger-in-needle, 0.5µL to 5µL 339 

Series 1700 Gas and liquid tight, 10µL to 500µL 340 

Series 1800 Gas and liquid tight with reinforced plunger, 10µL to 250µL 340 

Super Gas Gas tight, large volume acrylic, 500mL to 2000mL 340 

SampleLock 1000 and 1700 Series syringes with twist valve to lock sample in 341 

Autosampler 700 and 1700 Series syringes compatible with many popular autosamplers 341 

VICI ® -Precision Syringes 

Series A Gas tight with a twist valve for sample storage, 100µL to 10mL 343 

Series A-2 Gas tight with a push-button valve for sample storage, 25µL to 10mL 343 

SGE ® Syringes 

Standard General purpose, 5µL and 10µL 344 

Plunger Protection General purpose with reinforced plunger, 5µL and 10µL 344 

SuperfleX General purpose with flexible plunger, 5µL and 10µL 344 

Factory Calibrated NIST and NBS traceable, 5µL to 500µL 344 

Guided Plunger Extended barrel and reinforced plunger, 5µL and 10µL 344 

Gas Tight Gas and liquid tight, 10µL to 100mL 345 

Autosampler Syringes compatible with many popular autosamplers 345 

Microvolume Plunger-in-needle, gas and liquid tight, 0.5µL to 5µL 346 

On-Column On-column capillary GC injection, 0.5µL to 10µL 346 

Popper Syringes 

Standard Glass General purpose, 2mL to 50mL 347 

syringes 

tech tips 

Tips for Syringe Use and Care 

Cleaning Needles—If your syringe needle becomes clogged, do 

not force liquid or compressed air through the syringe. Excessive 

pressure can cause the glass barrel of the syringe to split. To 

properly clean the needle, remove the plunger from the syringe. 

Use cleaning wires to dislodge any foreign material or residue 

build-up, and rinse the syringe thoroughly with deionized water or 

acetone. Grace offers a variety of syringe care products to help 

you maintain the performance of your syringe on page 347. 

Sterilizing Syringes—Chemical sterilizers are the best means for 

sterilizing your syringe. Be sure to thoroughly rinse your syringe 

after sterilization to remove all traces of the chemical solution. In 

general, autoclaving your syringe is not recommended. Stainless 

steel expands faster than the glass upon heating. Heating a 

syringe with a cemented, stainless steel needle may cause the 

glass barrel to split. Heating a syringe with cemented steel hubs 

may result in adhesive deterioration and leakage. 

Syringe Lubrication—A clean syringe does not require any 

lubricating grease. Grease may cause a variety problems such as 

sample cross contamination, seizing of the plunger in the barrel, 

and barrel damage or breakage. 

7245 



Hamilton ® Selection Guide 

Syringe Terminations 

N—Cemented (fixed) Needle, up to 250µL 

Point Styles 

Point Style AS: 

N and LTN syringes have needles 

cemented into the glass syringe 

barrel. Do not heat to more than 

50°C, do not autoclave, do not use 

with halogenated solvents such 

as methylene chloride. Choose 

a cemented needle if you want 

reproducible injections at an 

economical price. 

LTN—Cemented (fixed) Needle with Luer Tip, 

500µL and larger 

4790 

4792 

Point Style 2: 



2593 

2589 

2590 

Special conical needle designed to 

withstand the demands of multiple 

injections; exclusively used on 

autosampler syringes. 

Beveled non-coring needle 

recommended for septum penetration; 

only needle gauges 26s–22 are 

recommended for optimum GC 

septum penetration. 

Blunt needle for use with HPLC 

injection valves and for sample 

pipetting. 

LT—Luer Tip 

Ground glass male luer tip accepts 

most hypodermic needles. Use Kel-F ® 

hubs and connectors for a tight, leakfree 

seal. Choose LT syringes if you 

want an autoclavable syringe with 

easy, fast needle replacement. 

4791 


2591 

2592 

10–12° beveled needle recommended 

for life science applications. 

Conical needle with side port for 

penetration of septa, thin-gauged 

vinyls and plastics without coring; 

minimizes septum damage. 

KH—Knurled Hub 

Metal knurled hub with removable 

needle, exclusive to 7000 Series 

syringes. It creates a leak-free seal 

between the barrel and the needle 

and can be tightened to compensate 

for wear. Autoclavable when 

disassembled. 

RN—Removable Needle 

Choose removable needles to easily 

replace bent or clogged needles or 

use different types of needles with 

the same syringe barrel. Autoclavable 

when disassembled. 

TLL—PTFE Luer Lock 

Male luer taper with nickel-plated 

brass hub that accepts and locks 

luer hub needles and connectors. 

Autoclavable when disassembled. 

Choose TLL syringes for fast needle 

replacement with a locking needle 

connection. 

4793 

4794 

4795 

Syringe Solution Reference Chart 

Hamilton has designed many unique syringes to address 

specific problems. Use the chart below to select the right 

syringe for the job. 

Syringe Problems and Solutions 

Problem Syringe Solution Hamilton ® 

Series 

Bending Plungers 

Use reinforced plunger 

syringes 

600, 800, 900, 

1800 

Breaking Syringe Barrels 

Bending Needles 

Use a Chaney adapter or 

syringe guide 

Use replaceable barrel 

syringes 

Use removable needle 

syringes 

700, 800, 900, 

1000, 1700, 

1800, 7000 

800, 1000, 1700, 

1800, 7000 

600, 700, 800, 

900, 1000, 1700, 

1800, 7000 

Syringe Balance Use syringe with handles 800, 900, 1800 

Plungers Pulling or 

Use pull or blow-out 800, 1800 

Blowing-out of Syringe Barrel prevention syringes 

Plungers Slide Too Easily Use adjustable plunger 

drag syringes 

800, 1800 

syringes 


337


syringes 


700 Series MICROLITER Syringes 

General Purpose Syringe, 

5µL to 500µL 

• Plungers are not 

interchangeable or replaceable 

• Accurate to 1% of the syringe volume 

Hamilton ® 700 Series Syringes 

Model Volume Gauge Pt. Style Repl. Needle Qty. Part No. 

Cemented Needle Syringes (N), 2" Needle Length 

75 5µL 26s 2 None ea 87900 

5µL 26s 3 None ea 87919 

701 10µL 26s 2 None ea 80300 

10µL 26s 3 None ea 803830 

10µL 26s 5 None ea 80339 

702 25µL 22s 2 None ea 80400 

25µL 22s 3 None ea 80465 

25µL 22s 5 None ea 80439 

705 50µL 22s 2 None ea 80500 

50µL 22s 3 None ea 80565 

50µL 22s 5 None ea 80539 

710 100µL 22s 2 None ea 80600 

100µL 22s 3 None ea 80665 

100µL 22s 5 None ea 80639 

725 250µL 22s 2 None ea 80700 

250µL 22s 3 None ea 80765 

250µL 22s 5 None ea 80739 

750 500µL 22 2 None ea 80800 

500µL 22 3 None ea 80865 

500µL 22 5 None ea 80839 

Removable Needle Syringes (RN), 2" Needle Length 

75 5µL 26s 2 7758-02 ea 87930 

701 10µL 26s 2 7758-02 ea 803300 

702 25µL 22s 2 7758-03 ea 80430 

705 50µL 22s 2 7758-03 ea 80530 

710 100µL 22s 2 7758-03 ea 80630 

725 250µL 22s 2 7779-03 ea 80730 

750 500µL 22 2 7779-01 ea 808301 

Luer Tip Syringes (LT) 

701 10µL — — * ea 80301 

702 25µL — — * ea 80401 

705 50µL — — * ea 80501 

710 100µL — — * ea 80601 

725 250µL — — * ea 80701 

750 500µL — — * ea 808011 

For Rheodyne ® , Valco ® VISF-2, Beckman ® /Altex and SSI, 2" Needle 

Length, Fixed Needle 

701 10µL 22s 3 None ea 80365 

702 25µL 22s 3 None ea 80465 

705 50µL 22s 3 None ea 80565 

710 100µL 22s 3 None ea 80665 

725 250µL 22 3 None ea 80765 

750 500µL 22 3 None ea 80865 

*Order needles separately, see page 342. 

4801 

Guide 

Plunger Guides reduce the 

expense of replacing or 

repairing syringes with bent 

plungers. They are especially 

useful on syringes with small 

diameter plungers. 

Chaney Adapter 

Chaney Adapters ensure 

consistent sample delivery. 

You will know the sample size 

is right every time. 

Guides and Chaney Adapters 

Description 

Part No. 

Guide for 701 14806 

Guide for 702–750, 7000 14906 

Chaney Adapter for 701, 75 147000 

Chaney Adapter for 702–750 and 7000 14725 


• Low price with 

a reinforced 

construction 


• Suitable for 

Rheodyne ® , 

Beckman ® /Altex, and 

Valco ® VISF-2 

Reinforced 

Plunger 


Model Volume Gauge Pt. Style Repl. Needle Part No. 


95 5µL 26s 2 None 87920 

901 10µL 26s 2 None 803600 


95 5µL 26s 2 7758-02 87925 

901 10µL 26s 2 7758-02 803700 

4800 

4799 

4808 

Save Money on Model 701 with Multi-Packs 

• Economical—syringes cost less in six packs 

• Easy identification—six numbered dots 

provide quick syringe identification 

best 

value 




62 2.5µL 22s 3 7770-01 87942 

65 5µL 22s 3 7770-01 87943 

4804 

Hamilton ® 700 Series Six-packs (2" Needle Length) 

Model Volume Gauge Pt. Style Repl. Needle Qty. Part No. 


701N 10µL 26s 2 None 6 80366 

701N 10µL 26s 2 None 12 803662 

701RN 10µL 26s 2 7758-02 6 80336 

338 www.discoverysciences.com 

4802




• General purpose 

syringe 

• Extended handle 

simplifies sampling 

from test tubes or 

long-necked flasks 

Reinforced 

Plunger 

1000 Series GASTIGHT Syringes 

Gas and Liquid Tight, 1mL to 100mL 

• PTFE-tipped plungers 

• Replaceable barrels and 

plungers 




85 5µL 26s 2 None 84850 

801 10µL 26s 2 None 84852 

802 25µL 22s 2 None 84854 

805 50µL 22s 2 None 84856 

810 100µL 22s 2 None 84858 


85 5µL 26s 2 7758-02 84851 

801 10µL 26s 2 7758-02 84853 

802 25µL 22s 2 7758-03 84855 

805 50µL 22s 2 7758-03 84857 

810 100µL 22s 2 7758-03 84859 

825 250µL 22s 2 7758-03 84861 

Removable Needle Syringes (RNW) 

For Waters ® U6K Injection Valve, 1.97" Needle Length 

801 10µL 25s 3 80426 84815 

802 25µL 25s 3 80426 84816 

805 50µL 25s 3 80426 84817 

810 100µL 25s 3 80426 84818 

825 250µL 25s 3 80726 84819 

7000 Series MODIFIED MICROLITER 

Syringes 

Plunger-in-Needle Design, 

0.5µL to 5µL 

• No dead volume 


plunger wires 

• Not recommended for viscous samples 



Knurled Hub Syringes (KH), 2.75" Needle Length 

7000.5 0.5µL 25 2 17887 86259 

0.5µL 25 3 17187 86250 

7000.50C* 0.5µL 32 3 86258 86257 

7001 1µL 25s 2 17886 80135 

1µL 25s 3 17188 80100 

7101 1µL 22s 2 17890 86211 

1µL 22s 3 17190 86200 

7002 2µL 25 2 17891 88411 

2µL 25 3 17191 88400 

7102 2µL 23 2 17892 88511 

2µL 23 3 17192 88500 

7105 5µL 24 2 17893 88011 

5µL 24 3 17193 88000 

*Needle length = 3.94" (2cm). 

4805 

best 

seller 

4803 




1001 1.0mL 22 2 7779-01 81330 

1002 2.5mL 22 2 7779-01 81430 

1005 5.0mL 22 2 7779-01 81530 

1010 10.0mL 22 2 7779-01 81630 

Luer Tip Syringes (LT), 2" Needle Length 

1001 1.0mL — — ** 81301 

1002 2.5mL — — ** 81401 

1005 5.0mL — — ** 81501 

1010 10.0mL — — ** 81601 

Luer Tip w/Cemented Needle Syringes (LTN), 2" Needle Length 

1001 1.0mL 22 2 None 81317 

1.0mL 22 5 None 81343 

1002 2.5mL 22 2 None 81417 

2.5mL 22 5 None 81443 

1005 5.0mL 22 2 None 81517 

5.0mL 22 5 None 81543 

1010 10.0mL 22 2 None 81617 

10.0mL 22 5 None 81643 

PTFE Luer Lock Syringes (TLL), 2" Needle Length 

1001 1.0mL — — ** 81320 

1002 2.5mL — — ** 81420 

1005 5.0mL — — ** 81520 

1010 10.0mL — — ** 81620 

1025 25.0mL — — ** 82520 

1050 50.0mL — — ** 85020 

1100 100.0mL — — ** 86020 

Removable Needle Syringes, 0.75" Needle Length 

For Valco ® (VISF-1) Injection Valves — Black 

1001 1mL 22 3 76515 81331 

1002 2.5mL 22 3 76515 81431 

1005 5mL 22 3 76515 81531 

1010 10mL 22 3 76515 81631 

For Rheodyne ® , Valco ® (VISF-2), Beckman ® /Altex, and SSI — Black 

1001 1mL 22 3 7780-04 81365 

Luer Tip w/Cemented Needle Syringes (LTN), 2" Needle Length For 

Rheodyne ® , Valco ® (VISF-2), Beckman ® /Altex, and Black SSI 

1001 1.0mL 22 3 None 81316 

1002 2.5mL 22 3 None 81416 

1005 5.0mL 22 3 None 81516 

1010 10.0mL 22 3 None 81616 

**Order needles separately, see page 342. 

4809 

syringes 

Guides and Chaney Adapters 

Syringe Model 

Part No. 

Guide Assembly for 7000 Series 14906 

Chaney Adapter for 7000 Series 14725 


339


syringes 



Gas and Liquid Tight, 10µL to 500µL 



plungers 

Hamilton ® 1700 Series GASTIGHT Syringes 



1701 10µL 26s 2 None 80000 

10µL 26s 3 None 800750 

10µL 26 3 None 800850 

10µL 26 5 None 80039 

1702 25µL 22s 2 None 80200 

25µL 22s 3 None 802750 

25µL 22s 5 None 80239 

25µL 22 3 None 80285 

1705 50µL 22s 2 None 80900 

50µL 22s 3 None 80975 

50µL 22s 5 None 80939 

50µL 22 3 None 80985 

1710 100µL 22s 2 None 81000 

100µL 22s 3 None 81075 

100µL 22s 5 None 81039 

100µL 22 3 None 81085 

1725 250µL 22s 2 None 81100 

250µL 22s 3 None 81175 

250µL 22s 5 None 81139 

250µL 22 3 None 81185 


1701 10µL 26s 2 7758-02 800300 

1702 25µL 22s 2 7758-03 80230 

1705 50µL 22s 2 7758-03 80930 

1710 100µL 22s 2 7758-03 81030 

1725 250µL 22s 2 7779-03 81130 

1750 500µL 22 2 7779-01 81230 

Luer Tip Syringes (LT) 

1701 10µL — — * 80001 

1702 25µL — — * 80201 

1705 50µL — — * 80901 

1710 100µL — — * 81001 

1725 250µL — — * 81101 

1750 500µL — — * 81201 

Luer Tip with Cemented Needle Syringes (LTN), 2" Needle Length 

1750 500µL 22 2 None 81217 

500µL 22 3 None 81216 

500µL 22 5 None 81243 

PTFE Luer Lock Syringes (TLL) / Without Slots 

1705 50µL — — * 80920 

1710 100µL — — * 81020 

1725 250µL — — * 81120 

1750 500µL — — * 81220 

*Order needles separately, see page 342. 

Hamilton ® 1700 Series GASTIGHT Syringes 


Removable Needle Syringes 

For Waters ® U6K Injection Valve (RNW), 1.97" Needle Length 

1702 25µL 25s 3 80426 80238 

1705 50µL 25s 3 80426 80938 

1710 100µL 25s 3 80426 81038 

1725 250µL 25s 3 80726 81138 

For Rheodyne ® , Valco ® VISF-2, Beckman ® /Altex, SSI (RNR), 

2" Needle Length 

1701 10µL 22s 3 7770-01 80065 

1702 25µL 22s 3 7770-01 80265 

4780 

Hamilton ® 1700 Series GASTIGHT Syringes (continued) 


For Rheodyne ® , Valco ® VISF-2, Beckman ® /Altex, SSI (RNR), 

2" Needle Length (continued) 

1705 50µL 22s 3 7770-01 80965 

1710 100µL 22s 3 7770-01 81065 

1725 250µL 22 3 7780-04 81165 

1750 500µL 22 3 7780-04 81265 

For Valco ® VISF-1 (RNCP), 0.75" Needle Length 

1702 25µL 22s 3 76517 80231 

1705 50µL 22s 3 76517 80931 

1710 100µL 22s 3 76517 81031 

1725 250µL 22s 3 76517 81131 

1750 500µL 22 3 76515 81231 


Gas and Liquid Tight with Reinforced Plunger, 


• Similar to the 1700 Series 

Reinforced 

but with a reinforced, 

Plunger 

protected plunger 


• Replaceable barrels and plungers 

Hamilton ® 1800 Series Syringes and Accessories 



1801 10µL 26s 2 None 84875 

1802 25µL 22s 2 None 84878 

1805 50µL 22s 2 None 84881 

1810 100µL 22s 2 None 84884 

1825 250µL 22s 2 None 84887 


1801 10µL 26s 2 7758-02 84877 

1802 25µL 22s 2 7758-03 84880 

1805 50µL 22s 2 7758-03 84883 

1810 100µL 22s 2 7758-03 84886 

1825 250µL 22s 2 7779-03 84889 

Removable Needle Syringes (RNW) 

For Waters ® U6K Injection Valve, 1.97" Needle Length 

1802 25µL 25s 3 80426 84980 

1805 50µL 25s 3 80426 84983 

1810 100µL 25s 3 80426 84986 

1825 250µL 25s 3 80726 84989 

Hamilton Accessories 

Chaney Adapter for 801-825, 1801-1825 32146 

Super Gas Syringes 

Gas Tight, Large Volume 

Syringes, 500mL to 2000mL 

• Ideal for air and gas sampling 

• Graduated every 20mL 

• Acrylic barrels with PTFE-coated 

aluminum pistons 

Hamilton ® Super Gas Syringes 

Model Volume Part No. 

Syringe with PTFE Luer Lock (TLL) 

S-0500 0.5 Liter 86311 

S-1000 1.0 Liter 86312 

S-1500 1.5 Liter 86313 

S-2000 2.0 Liter 86314 

4781 

4783 




Autosampler Syringes 

For the Agilent 7673 and 7683 Autosamplers 

• Standard needle length is 1.71" 

Syringes for the Agilent 7673 and 7683 Autosamplers 

Model Volume Gauge Pt. Style Qty. Part No. 

Cemented Needle Syringes (ASN) 

75 5µL 23s AS ea 87987 

5µL 23s AS 6 87990 

5µL 23s 2 ea 87991 

5µL 26s AS ea 87988 

5µL 26s AS 6 87989 

5µL 26s 2 ea 87992 

5µL 23s-26s AS ea 87993 

5µL 23s-26s AS 6 87994 

701 10µL 23s AS ea 803871 

10µL 23s AS 6 803901 

10µL 23s 2 ea 80398 

10µL 26s AS ea 803881 

10µL 26s AS 6 803891 

10µL 26s 2 ea 80399 

10µL 23s-26s AS ea 80393 

10µL 23s-26s AS 6 803911 

Removable Needle Syringes (ASRN) 

75 5µL 23s AS ea 87957 

5µL 26s AS ea 87958 

5µL 23s-26s AS ea 87959 

701 10µL 23s AS ea 80357 

10µL 26s AS ea 80358 

10µL 23s-26s AS ea 80359 

Cemented Needle Gastight Syringes (ASN) 

175 5µL 23s AS ea 800741 

5µL 23s AS 6 80090 

5µL 23s-26s AS ea 800761 

5µL 23s-26s AS 6 80092 

1701 10µL 23s AS ea 800801 

10µL 23s AS 6 80094 

10µL 23s-26s AS ea 800791 

10µL 23s-26s AS 6 80096 

Removable Needle Gastight Syringes (ASRN) 

175 5µL 23s-26s AS ea 80086 

1701 10µL 23s AS ea 80087 

10µL 23s-26s AS ea 80089 

For the Agilent 7670, 7671, and 7672 

Autosamplers 

Syringes for Agilent 7670, 7671, 7672 Autosamplers 

Model Volume Gauge Pt. Style Qty. Part No. 

Cemented Needle Syringes (N), 2" Needle 

701N 10µL 26s 2 ea 80300 

10µL 26s 2 6 80366 

Removable Needle Syringes (RN), 2" Needle 

701 10µL 26s 2 ea 80338 

Cemented Needle with PTFE-Tipped Plungers (N), 2" Needle 

1701 10µL 26s 2 ea 80000 

Removable Needle with PTFE-Tipped Plungers (RN), 2" Needle 

1701 10µL 26s 2 ea 800110 

For the PerkinElmer ® Autosystem 

Syringes for the PerkinElmer ® Autosystem 

Model Volume Needle o.d. Needle Lgth. Pt. Style Part No. 

Cemented Needle Syringes (ASN/PE) 

75 5µL 0.47mm 2.756" 3 880401 

5µL 0.63mm 2.756" 3 880351 

SampleLock Syringes 

• 1000 and 1700 Series 

syringes with twist valve 

Hamilton ® SampleLock Syringes 


SampleLock Syringes, 2" Needle Length 

1705 50µL 22s 2 7779-03 80956 

1710 100µL 22s 2 7779-03 81056 

1725 250µL 22s 2 7779-03 81156 

1750 500µL 22 2 7779-01 812560 

1001 1mL 22 2 7779-01 81356 

1002 2.5mL 22 2 7779-01 81456 

1005 5mL 22 2 7779-01 81556 

1010 10mL 22 2 7779-01 81656 

1025 25mL 22 2 7779-01 863260 

1050 50mL 22 2 7779-01 863360 

1100 100mL 22 2 7779-01 86346 

6611 

Replacement Needles for Agilent Autosamplers 

• For 5 and 10µL syringes 

Agilent Autosampler Replacement Needles 

Gauge Needle Length Pt. Style Qty. Part No. 

23s-26s 1.71" AS 6 7785-01 

23s 1.71" AS 6 7786-01 

26s 1.71" AS 6 7786-02 

Valves and Adapters 

syringes 

For the Waters ® WISP Autosampler 

Syringes for the Waters ® WISP Autosampler 

Model Volume Part No. 

Gastight Syringes with PTFE-Tipped Plungers 

1725 WISP 250µL 80024 

more info 

See page 195 for Hamilton ® TLC Syringes. 

4812 4811 4810 

Valves and Adapters 


Sampling Valve 

Inert Gas Sampling Valve 86580 

Luer Adapters 

Female Luer to Removable Needle Hub 35081 

Male Luer to Removable Needle Hub 35080 

Male Luer Lock to Removable Needle Hub 35083 

Septum Adapter 

Septum Adapter (TLL), Includes 12 Septa ea 313350 

Replacement Septa, 7mm 12 75810 


341


syringes 

Hamilton ® Needles 

RN Replacement Needle Packs 

• For syringes designated “RN” 

• Needle length is 2" 

RN Replacement Needles 

5-100μL 

Part No. 

250μL-10mL 

Part No. 

Gauge Pt. Style Qty. 

RN Needles for 5µL — 100µL Syringes 

22 2 6 7758-01 7779-01 

22 3* 6 7770-02 7780-04 

22 5 6 7784-06 7784-04 

22s 2 6 7758-03 7779-03 

22s 3* 6 7770-01 7780-03 

22s 5 6 7784-05 7784-03 

26 2 6 7758-04 7779-04 

26 3 6 7768-02 7780-02 

26 5 6 7784-08 7784-02 

26s 2 6 7758-02 7779-02 

26s 3 6 7768-01 7780-01 

26s 5 6 7784-07 7784-01 

*Compatible with Rheodyne ® Valves. 

Replacement Needles for HPLC Syringes 

HPLC Replacement Needles 

Gauge Length Pt. Style For Valve Qty. Part No. 

RN Needles for 5µL — 100µL Syringes 

22s 0.75" 3** Valco ® 6 7787-01 

22s 2" 3* Rheodyne ® 6 7770-01 

25s 1.97" 3*** Waters ® 6 8647-01 

RN Needles for 250µL — 10mL Syringes 

22 0.75" 3** Valco ® 6 7787-02 

22 2" 3* Rheodyne ® 6 7780-04 

22s 0.75" 3** Valco ® 3 76518 

22s 2" 3* Rheodyne ® 6 7780-03 

25s 1.97" 3*** Waters ® 6 8648-01 

CTFE Luer Hub Needles 

22 2" 3* Rheodyne ® 6 90536 

22s 2" 3* Rheodyne ® 6 90534 

*Compatible with Rheodyne ® , Beckman ® /Altex, SSI, and Valco ® Model VISF-2 Valves; 

**Compatible with Valco ® Valves; ***Compatible with Waters ® Valves. 

tech tip 

Needle Gauge vs. o.d.-i.d. 

Gauge o.d. i.d. 

33 0.008" 0.2032mm 0.004" 0.1016mm 

32 0.009" 0.2286mm 0.004" 0.1016mm 

31 0.010" 0.2540mm 0.005" 0.1270mm 

30 0.012" 0.3048mm 0.006" 0.1524mm 

29 0.013" 0.3302mm 0.007" 0.1778mm 

28 0.014" 0.3556mm 0.005" 0.1270mm 

27 0.016" 0.4064mm 0.008" 0.2032mm 

26s 0.0185" 0.4699mm 0.0045" 0.1143mm 

26 0.018" 0.4572mm 0.010" 0.2540mm 

25 0.020" 0.5080mm 0.010" 0.2540mm 

24 0.022" 0.5588mm 0.012" 0.3048mm 

23 0.025" 0.6350mm 0.013" 0.3302mm 

22 0.028" 0.7112mm 0.016" 0.4064mm 

22s 0.028" 0.7112mm 0.006" 0.1524mm 

21 0.032" 0.8128mm 0.020" 0.5080mm 

20 0.035" 0.8890mm 0.023" 0.5842mm 

19 0.042" 1.0668mm 0.027" 0.6858mm 

18 0.049" 1.2446mm 0.033" 0.8382mm 

17 0.058" 1.4732mm 0.042" 1.0668mm 

16 0.065" 1.6510mm 0.047" 1.1938mm 

15 0.077" 1.9558mm 0.054" 1.3716mm 

Standard Luer Hub Needles 

• Will fit any luer or luer lock hub 

• Needle length is 2" 

• All needles are 304 SS 

Metal Luer Needles 

• Economical 

Metal Hub 

Kel-F Hub 

Standard Luer Hub Needles 

Needle Metal Hub Kel-F ® Hub 

Gauge Pt. Style Qty. Part No. Part No. 

16 2 6 N-716 KF-716 

16 3 6 91016 90516 

16 5 6 7729-12 7746-01 

17 2 6 N-717 KF-717 

17 3 6 910170 90517 

17 5 6 7729-11 7746-02 

18 2 6 N-718 KF-718 

18 3 6 910180 90535 

18 5 6 7729-10 7746-03 

20 2 6 N-720 KF-720 

20 3 6 910200 905200 

20 5 6 7729-09 7746-04 

21 2 6 N-721 KF-721 

21 3 6 910210 90521 

21 5 6 7729-08 7746-05 

22 2 6 N-722 KF-722 

22 3* 6 91022 90536 

22 5 6 7729-07 7746-06 

22s 2 6 N-722s KF-722s 

22s 3* 6 910380 90534 

22s 5 6 7729-02 7746-11 

23 2 6 N-723 KF-723 

23 3 6 910230 90523 

23 5 6 7729-06 7746-07 

24 2 6 N-724 KF-724 

24 3 6 91024 90524 

24 5 6 7729-05 7746-08 

25 2 6 N-725 KF-725 

25 3 6 910250 90525 

25 5 6 7729-04 7746-09 

26 2 6 N-726 KF-726 

26 3 6 91026 90533 

26 5 6 7729-03 7746-10 

26s 2 6 N-726s KF-726s 

26s 5 6 7729-01 7746-12 

*Compatible with Rheodyne ® Valves. 

Metal Luer Needles 

Description Gauge Length Qty. Part No. 

Beveled Point 

Metal Luer 18 1.5" 12 90096 

Metal Luer 22 1.5" 12 90100 

more info 

For more replacement parts for 

Hamilton ® Syringes, please visit 

our website. 

4797 

4798 

5048 

342 



Vici ® Precision Syringes 

Point Styles 

Point Style 2 

2645 

VICI ® Precision 


Replacement Plunger Tips and Needles 

Gas Syringe Features 

Pressure Removable Luer Valve Plunger 

Series Rating Needle Needle Type Stop 

A 250psig X Twist-Lock X 

A-2 250psig X X Push-Button X 

Series A Syringes 

Gas Tight with a Twist Valve, 100µL to 10mL 

• PTFE-tipped plunger and 

needle seal 

• Twist valve stores sample in barrel 

Series A Syringes 

Volume Ndl. o.d. Pt. Style Repl. Needle Part. No. 

Removable Needle Syringes, 2.25" Needle Length 

100µL 0.029" 2 913050 010025 

250µL 0.029" 2 913050 010031 

500µL 0.029" 2 913050 010032 

1mL 0.029" 2 913050 010033 

2mL 0.029" 2 913050 010034 

5mL 0.029" 2 913050 010035 

10mL 0.029" 2 913050 010036 

5041 

Replacement PTFE Plunger Tips 

Description 

Part No. 

For Series A and A-2 Syringes 

100µL 16192 

250µL 16194 

500µL 013932 

1mL 013933 

2mL 013934 

5mL 013935 

10mL 013936 

Replacement Needles 3/pk 

Fit Models Ndl. o.d. Ndl. i.d. Ndl. Lgth. Pt. Style Part. No. 

Removable Needles 

A 0.029" 0.012" 2.25" 2 913050 

0.029" 0.012" 2.25" 3 913052 

A-2 0.028" 0.005" 2" 2 943050 

0.029" 0.012" 2" 2 943051 

0.029" 0.012" 2" 3 943052 

Luer Needles 

A-2 0.028" 0.006" 2" 2 943060 

0.028" 0.016" 2" 2 943061 

0.028" 0.016" 2" 3 943062 

Series A-2 Syringes 

Gas Tight with a Push Valve, 25µL to 10mL 

• PTFE-tipped plunger and 

needle seal 

• Push-button valve stores 

sample in barrel 

Series A-2 Syringes 

Volume Needle o.d. Pt. Style Repl. Needle Part. No. 

Removable Needle Syringes, 2" Needle Length 

25µL 0.028" 2 943050 050023 

50µL 0.028" 2 943050 050024 

100µL 0.028" 2 943050 050025 

250µL 0.029" 2 943051 050031 

500µL 0.029" 2 943051 050032 

1mL 0.029" 2 943051 050033 

2mL 0.029" 2 943051 050034 

5mL 0.029" 2 943051 050035 

10mL 0.029" 2 943051 050036 

Luer Syringes — Needle Supplied, 2" Needle Length 

25µL 0.028" 2 943060 16014 

50µL 0.028" 2 943060 16016 

100µL 0.028" 2 943060 16018 

250µL 0.028" 2 943061 16023 

500µL 0.028" 2 943061 16025 

1mL 0.028" 2 943061 16027 

2mL 0.028" 2 943061 16029 

5mL 0.028" 2 943061 16032 

10mL 0.028" 2 943061 16034 

best 

seller 

5040 

Mininert ® Syringe Valves 

• Add storage capability to standard 

syringes 

Mininert ® Valves 

Description 

Part No. 

To Fit Any Standard Luer Tip Syringes 654051 

more info 

For our full VICI ® Precision offering, including replacement 

parts and other syringes not listed here, visit the product portion 

of our website. 





5045 

syringes 


343


syringes 


Point Styles 

Point Style 1 

Point Style 2 

Point Style 3 

1503 

1504 

1505 

Plunger Protection Syringes 

#1 Cone Needle Tip – Designed 

for autosamplers. Recommended 

for use with pre-drilled septa. 

#2 20° Bevel Needle Tip – Standard general 

purpose needle. 

#3 HPLC Needle Tip – 90° square cut with 

chamfered and polished edges. Use with 

HPLC valves. 

General Purpose Syringes with Reinforced Plunger, 

5µL and 10µL 

• Includes certificate of conformance to NIST 

SGE ® Plunger Protection Syringes 

Volume Gauge Length Pt. Style Repl. Needle Qty. Part No. 

Fixed Needle Syringes 

5µL 26 5cm 2 None ea 85184 

10µL 26 5cm 2 None ea 85056 

10µL 26 5cm 2 None 6 85190 

10µL 26 5cm 2 None 10 85400 


5µL 26 5cm 2 85272 ea 85186 

10µL 26 5cm 2 85294 ea 85050 

10µL 26 5cm 2 85294 6 85194 

Standard Syringes 

General Purpose Syringes, 5µL and 10µL 

• Includes certificate of conformance to NIST 

SGE ® Standard Syringes 


Fixed Needle Syringes for Use with Merlin Microseal ® 

5µL 23 4.2cm 1 None ea 85005 

10µL 23 4.2cm 1 None ea 85003 


25µL 25 5cm 2 None ea 85058 

50µL 25 5cm 2 None ea 85002 

100µL 25 5cm 2 None ea 85012 

250µL 25 5cm 2 None ea 85060 

500µL 25 5cm 2 None ea 85062 


25µL 25 5cm 2 85274 ea 85164 

50µL 25 5cm 2 85274 ea 85008 

100µL 25 5cm 2 85274 ea 85006 

250µL 25 5cm 2 85274 ea 85052 

500µL 25 5cm 2 85274 ea 85054 

more info 

For our full SGE ® offering, visit the product portion of our website. 

SuperfleX Syringes 

Syringes with Flexible Plungers, 5µL and 10µL 

• Includes plunger protection guide 

SGE ® SuperfleX Syringes 



5µL 26 5cm 2 None ea 85196 

10µL 26 5cm 2 None ea 85206 

10µL 26 5cm 2 None 6 85208 

10µL 26 7cm 2 None ea 85185 


5µL 26 5cm 2 85272 ea 85200 

10µL 26 5cm 2 85294 ea 85210 

10µL 26 5cm 2 85294 6 85212 

NIST Syringes 

NIST and NBS Traceable, 


• Includes certificate 

of calibration 

SGE ® Factory Calibrated Syringes 

Volume Gauge Length Pt. Style Repl. Needle Part No. 

Fixed Needle Syringes with Repeating Adapter 

5µL 26 5cm 2 None 85015 

10µL 26 5cm 2 None 85017 

25µL 22 5cm 3 None 85023 

50µL 25 5cm 2 None 85025 

100µL 25 5cm 2 None 85027 

Guided Plunger Syringes 

Extended Barrel Syringes, 5µL and 10µL 

• Reinforced plunger 

SGE ® Guided Plunger Syringes 



5µL 26 5cm 2 None 85022 

10µL 26 5cm 2 None 85038 


5µL 26 5cm 2 85272 85016 

10µL 26 5cm 2 85294 85018 

10µL 26 7cm 2 85163 85159 

4913 

4905 

4901 




Gas Tight Syringes 

Gas and Liquid Tight, 

10µL to 100mL 

• Replaceable PTFE-tipped plunger 

best 

seller 

Autosampler Syringes 

4907 

SGE ® Gas Tight Syringes (Needles sold separately, see page 346) 

Volume Needle o.d. Length Pt. Style Part No. 

Fixed Luer Lock 

50µL — — — 85357 

100µL — — — 85359 

250µL — — — 85361 

500µL — — — 85363 

1mL — — — 85080 

2.5mL — — — 85123 

Removable Luer Lock 

5mL — — — 85088 

10mL — — — 85092 

25mL — — — 85125 

50mL — — — 85096 

100mL — — — 85098 

Luer Lock with Valve 

1mL — — — 85127 

2.5mL — — — 85131 

5mL — — — 85133 

10mL — — — 85135 

20mL — — — 85137 

50mL — — — 85139 

100mL — — — 85143 

SGE ® Gas Tight Syringes, Needles included 


Fixed Needle 

10µL 26 5cm 2 None 85064 

10µL 26 7cm 2 None 85066 

25µL 25 5cm 2 None 85321 

50µL 25 5cm 2 None 85323 

100µL 25 5cm 2 None 85325 

250µL 25 5cm 2 None 85327 

500µL 25 5cm 2 None 85329 

Removable Needle 

10µL 26 5cm 2 85294 85004 

25µL 25 5cm 2 85274 85331 

50µL 25 5cm 2 85274 85070 

100µL 25 5cm 2 85274 85072 

250µL 25 5cm 2 85274 85074 

500µL 25 5cm 2 85274 85076 

1mL 23 5cm 2 85276 85078 

2.5mL 23 5cm 2 85276 85117 

5mL 23 5cm 2 85278 85086 

10mL 23 5cm 2 85278 85090 

4905 

SGE ® Autosampler Syringes 

Volume Gauge Pt. Style Repl. Needle Qty. Part No. 

for Agilent 7673 Autosamplers 

Fixed Needle 

5µL 23 1 None ea 85014 

5µL 23 1 None 6 85140 

5µL 26 1 None ea 85136 

5µL 26 1 None 6 85138 

10µL 23 1 None ea 85144 

10µL 23 1 None 6 85036 

10µL 26 1 None ea 85142 

10µL 26 1 None 6 85034 


0.5µL 23 1 85292 ea 85134 

0.5µL 26 1 85262 ea 85132 

5µL 23 1 85298 ea 85248 

5µL 26 1 85296 ea 85394 

10µL 23 1 85302 ea 85148 

10µL 26 1 85300 ea 85146 

25µL 23 1 85040 ea 85047 

50µL 23 1 85040 ea 85416 

for PerkinElmer ® Autosystem 

Fixed Needle 

5µL 23 1 None ea 85069 

5µL 26 1 None ea 85067 

50µL 23 1 None ea 85419 

Gas Tight Fixed Needle 

5µL 23 1 None ea 85160 

5µL 26 1 None ea 85158 


0.5µL 23 1 85308 ea 85156 

0.5µL 26 1 85306 ea 85154 

for Shimadzu ® Autosamplers 

Models AOC14 and 17—Removable Needle 

0.5µL 26 1 85312 ea 85166 

10µL 26 1 85316 ea 85172 

for Varian ® Autosamplers 

Model 8000—Removable Needle 

10µL 25 5 85167 ea 85176 

Models 8035, 8100, 8200—Removable Needle 

10µL 25 5 85173 ea 85180 

for CTC/Fisons AS200 and AS800 Autosamplers 

Fixed Needle (50mm) 

10µL 26 1 None ea 85297 

25µL 26 1 None ea 85401 

Fixed Needle (80mm) 

10µL 26 1 None ea 85398 

Gas Tight Fixed Needle (50mm) 

10µL 23 1 None ea 85399 

25µL 23 1 None ea 85402 

syringes 

Repeating Adapters 

• Preset to any desired volume 

SGE ® Repeating Adapters 

Description 

Part No. 

RA-6 for 0.5, 5, 10µL Syringes 85263 

RA-8 for 25-500µL Syringes 85265 

4902 


345


syringes 


Micro Syringes 

• Plunger-in-needle, 0.5µL to 5µL 

• Liquid and gas tight to 650 atmospheres* 

SGE ® Microvolume Syringes 

Volume Gauge Length Pt. Style Repl. Needle/Plunger Part No. 

Standard Barrel — 8mm o.d. 

0.5µL 23 7cm 1 85266 85028 

0.5µL 23 7cm 2 85235 85209 

0.5µL 26 7cm 1 85237 85039 

1µL 23 5cm 1 85268 85000 

1µL 23 7cm 1 85284 85030 

1µL 23 7cm 2 85243 85211 

1µL 26 7cm 1 85245 85213 

5µL 23 5cm 1 85247 85215 

5µL 23 7cm 1 85270 85032 

5µL 23 7cm 2 85253 85217 

Standard Barrel with Repeating Adapter 

0.5µL 23 7cm 1 85266 85219 

1µL 23 5cm 1 85268 85223 

1µL 23 7cm 1 85284 85225 

1µL 26 7cm 1 85245 85227 

5µL 23 5cm 1 85247 85229 

5µL 23 7cm 1 85270 85231 

*Use a repeating adapter to prevent plunger blow-out at elevated pressures. 

HPLC Syringes 

• Syringes and needles sold separately 

SGE ® HPLC Syringes 

Volume Gauge Length Pt. Style Part No. 

Luer Lock Syringes 

1mL — — — 85080 

2.5mL — — — 85123 

5mL — — — 85088 

10mL — — — 85092 

25mL — — — 85125 

HPLC Injector Specific Luer Lock Needles (2/pk) 

For Rheodyne ® /Valco ® 22 2" 3 85151 

For Waters ® U6K 24 1.97" 3 85153 

On-Column Syringes 

• 0.5µL to 10µL 

5038 

best 

seller 

SGE ® On-Column Syringes 

Volume Needle o.d. Length Repl. Needle Part No. 

On-Column Syringes, Fused Silica Needles 

5µL 0.17mm 10cm 85365 85382 

10µL 0.17mm 10cm 85367 85384 

For 0.53mm i.d. Capillary Column, Stainless Steel Needles 

0.5µL 0.47mm 7cm 85237 85039 

1µL 0.47mm 7cm 85245 85213 

4911 

4910 

SGE ® Accessories 

Syringe Valves 

Model VLL 

5052 

Model VLL — Connect to any SGE ® removable needle 

syringe 25µL–2.5mL. Luer lock fitting cone with Kel-F ® core. 

Model VLLMA — Designed specifically for the SGE ® larger 

capacity luer lock syringes 5mL–100mL. 

Model SLLV — Can be fitted to any luer lock or luer tip 

syringe or device. 

Replacement Needles 

5053 

Model VLLMA 

Model SLLV 

SGE ® Retro-Fit Syringe Valves, Use Luer Hub Needles 

Description 

Part No. 

Model VLL 85043 

Model VLLMA 85049 

Model SVLL 85055 

SGE ® Replacement Needles 

SGE No. o.d. Needle Length Style Qty. Part No. 

For 5µL Syringes 

036110 0.47mm 5cm 2 5 85272 

036710 0.47mm 4.2cm 1 2 85296 

036720 0.63mm 4.2cm 1 2 85298 

For 10µL Syringes 

037011 0.63mm 5cm 1 2 85364 

037110 0.47mm 5cm 2 5 85294 

037715 0.47mm 4.2cm 1 2 85300 

037736 0.47mm 5cm 1 2 85197 

037745 0.47mm 5cm 1 2 85316 

037747 0.63mm 5cm 1 2 85334 

037785 0.47mm 5cm 1 2 85317 

For 25 – 500µL Syringes 

038110 0.5mm 5cm 2 5 85274 

038250 0.028" 2" 3 5 85332 

038260 0.5mm 5cm 3 5 85338 

038810 0.63mm 5cm 1 ea 85377 

For 1–2.5mL Syringes 

039110 0.63mm 5cm 2 5 85276 

For 5 –10mL Syringes 

031516 0.63mm 5cm 2 5 85278 

For Luer Lock and Luer Tip Syringes 

039802 0.63mm 5cm 2 5 85287 

039803 0.63mm 5cm 5 2 85145 

039822 1.07mm 5cm 2 5 85289 

039823 1.07mm 5cm 5 2 85147 

039862 1.67mm 5cm 2 5 85149 

039895 0.028" 2" 3 2 85151 

039897 0.5mm 5cm 3 2 85153 

On-Column Needles 

036675 0.23mm 7.5cm 1 2 85369 

036625 0.17mm 11cm 1 2 85373 

5054 



Popper Glass Syringes 

• All-glass plunger and barrel 

• Corrosion and heat resistant 

• Flat flanges prevent rolling 

• Needles sold separately 

Syringe Accessories 

Redi-Cut Fine Wires 

• Rust-proof stainless steel 

4670 

Interchangeable Glass Syringes 


Glass Male Luer Tip 

2cc Volume ea 3663 






Metal Male Luer Lock 







5046 

Redi-Cut Fine Wires and Handle 

Description 

Part No. 

Redi-Cut Fine Wires, 10 each of diameters 0.003", 0.005", 4046 

0.010", 0.020" 

Handle 8184 

Syringe Cleaning Solutions 

• Syringe Kleen removes metal deposits 

from metal plungers 

• SK-2 soaking solution for hard-toremove 

silicones and organic residues 

Cleaning Solutions 

Description 

Part No. 

Syringe Kleen, 8oz 2030 

Syringe Kleen, 4 Bottles/pk 20304 

SK-2, 8oz 2040 

SK-2, 6 Bottles/pk 20406 

4671 

5047 

Needle Sharpener 

Serial Numbered Glass Syringes 


Glass Male Luer Tip 







Metal Male Luer Lock 







• Protect GC septa by 

sharpening needle in groove 

Needle Sharpener 

Description 

Part No. 

Needle Sharpener 2033 

Plastic Syringes 

• Use luer hub needles—sold 

separately (refer to page 342) 

4674 

syringes 

more info 

Use needles on page 342 with 

Popper Syringes. 

Plastic Syringes 


Plastic Syringes with a Luer Tip 

1mL Syringe 12 44701100 

5mL Syringe 12 447012 

20mL Syringe 2 447018 

4675 

4798 


347


Vials Introduction 

Your sample, the type of vial, cap and septa, the solvent used, and the type of autosampler and detector all contribute to the end 

quality of your analysis. Choose from our wide selection of vial options to get the right vial for your analysis. 

Screw Thread 

Standard Mouth 

Wide Mouth 

R.A.M.— for Robotics 

Clear or Amber 



Crimp Style 

Standard Mouth 

Wide Mouth 

Snap Seal 

Snap Ring 





Additional Vial Options 

Limited Volume 

High Recovery 

Shell Vials 

Limited Volume Inserts 

with molded 

small-volume 

shaped inside. 

Choice of crimp 

or screw thread. 

with low dead 

volume internal 

taper. Choice of 

crimp or screw 

thread. 

Thin-walled 

cylinders 

For 4 or 6mm i.d. vials. 

Top Spring 

Straight 

sides 

Versa-vial w/ 

pick-up neck 

Bottom Spring 

Conical 

Flat 

Bottom 

Flanged Top 

Step Insert 

Self-Centering 

Polyethylene 

Springs 

vials 

Properties of Plastics 

LDPE 

(Low-Density 

Polyethylene) 

HDPE 

(High-Density 

Polyethylene) 

PP 

(Polypropylene) 

PMP(TPX) 

(Polymethyl-pentene) 

PS 

(Polystyrene) 

PTFE 

Max. Temperature 80°C/176°F 120°C/248°F 135°C/275°F 175°C/347°F 90°C/194°F 260°C/500°F 

Transparency Translucent Translucent Translucent Clear Clear Opaque 

Sterilization Method 

Autoclaving No No Yes Yes No Yes 

Disinfectants Yes Yes Yes Yes No Yes 

Dry Heat No No No Yes No Yes 

Radiation Yes Yes No No Yes Yes 

Specific Gravity 0.92 0.95 0.90 0.83 1.05 2.14–2.24 

Flexibility* Good Rigid Rigid Rigid Rigid Rigid 

*Depends on thickness. 

Chart is general guideline only. Always test your selection using your test conditions. 

Borosilicate Glass Properties 

Clear 

Amber 

Linear Coefficient of Expansion: 33 51 

Strain Point (Max. Use Temp.): 515°C 535°C 

USP Class Type: Type 1 Type 1 

Light Protection: No Yes 

Note: Vials in this catalog are not recommended or designed for use with elevated 

temperatures, pressures, or vacuum reactions. This restriction includes vials that use 

the term “pressure” or “reaction” in the description. 

Vial Volumes vs. Dimensions 

Volume 

Dram 

Dimensions 

(o.d. x Height) 

2mL 0.5 12 x 32mm 

4mL 1 15 x 45mm 

7mL 2 17 x 60mm 

15mL 4 21 x 70mm 

22mL 6 23 x 85mm 

40mL 10.7 29 x 81mm 



Vial Introduction 

Septum Materials Capability 

Capability Chart 

Natural 

(cispolyisoprene) 

Butyl 

(isobutylene isoprene) 

Silicone 

(polysiloxane) 

PTFE 

(polytetra fluorocarbon) 

Fluoroelastomer Polymer 

(fluorocarbon) 

Property: 

Specific Gravity (g/cc) 0.93 0.9 1.1–1.6 2.14–2.24 1.4–1.95 

Minimum Use Temp. °C -51 -53 -84 -65 -53 

Maximum Use Temp. °C 82 149 204 260 260 

Hardness (Durometer) 30–90 40–90 30–90 50–65 65–90 

Tear Resistance Excellent Good Fair — Poor to Fair 

Abrasion Resistance Excellent Good to Excellent Poor — Good 

Resealability Good Good Excellent Poor Fair 

Chemical Resistance: 

Oxidation Good Excellent Excellent Excellent Excellent 

Heat Aging Good Excellent Excellent Excellent Excellent 

Solvents 

Aliphatic Hydrocarbons Poor Poor Fair Excellent Excellent 

Aromatic Hydrocarbons Poor Poor Poor Excellent Excellent 

Alcohols Good Very Good Excellent Excellent Excellent 

Oil, Gasoline Poor Poor Poor Very Good Very Good 

Animal, Vegetable Oils Poor to Good Excellent Excellent Very Good Very Good 

Acids Fair to good Excellent Very Good Excellent Good to Excellent 

Alkalies — — — Excellent Poor to Good 

Permeability to Gases Low Very Low High — Low 

Water-swell Resistance Fair Excellent Excellent Excellent Excellent 

Note: 1. Chemical resistance data are general guides only. Many factors such as temperature, chemical combinations, concentration, pressure, exposure time, etc. can influence 

or alter the resistance characteristics of a product. Always test your selection using your test conditions. As a general rule, as temperature increases, resistance to attack decreases. 

2. Combination septum (Ex. PTFE/Silicone) has the resistance of the facing material (PTFE) prior to puncture. After puncture, a small portion of the other material is exposed and its 

sealing and resistance characteristics must then be considered. 3. Colored closures and septa are free of heavy metals to comply with CONEG standards. 

How to Measure for Vial, Cap, and Septum Selection 

Bottles and caps are described with a two-part number system 

referred to as the Finish*. (Example: 13/425). The first number 

represents the neck size of the vial or cap in millimeters. 

The vial is measured from the outside edge of the threads on 

one side to the outer edge of the threads on the opposite side 

(Figure 1). The cap is measured from one side of the inner 

wall to the opposite side of the inner wall (Figure 2). 

Septum size can be determined by comparing septa or crimp 

seals to the measured circles (Figure 4). 

Actual Size 

18mm 

8mm 

20mm 

11mm 

22mm 

13mm 

24mm 

15mm 

Figure 4 


The second number describes the thread pattern and assures 

the bottle and cap will fit together (Figure 3). 

400 425 350 430 415 

With combination septa (Ex: PTFE/silicone, 5/55), the 

numbers represent the thickness of the individual components 

of the septum in mils. This same septum could be described 

as 0.060" or 60 mil. 

Durometer or hardness of septum material is referred to as 

“shore” and is an important consideration in working with 

some autosamplers (45-60 shore is the typical range for 

autosampler septa). Septa with slits or molded starburst 

patterns (Figure 5) allow easier needle penetration which can 

prevent needles bending or jamming on the autosampler arm. 

vials 

Figure 3 

*Neck finish specifications of both caps and vials are controlled by the GCMI-Glass 

Container Manufacturers Institute. 

Septum with 

Starburst 

Figure 5 

Septum with Slit 


349


Actual Size Drawings of Vials with Fill Volumes 

12x32mm Standard Mouth Vials and Inserts 

1.5mL 

C-66002 

A-95221 

12mm 

32mm 

1.5mL 

C-95191 

A-95194 

12mm 

32mm 

100 

mL 

27mm 

4mm 

95203 

100 

µL 

30mm 

5mm 

98107 

12x32mm WIDE Mouth Vials & Inserts 

200 

µL 

30mm 

5mm 

98028 

PP-99176 

100 

µL 

31mm 

5mm 

C-95201 

PP-98137 

50 

µL 

25mm 

4mm 

98018 

100 

µL 

30mm 

5mm 

C-98031 

PP-98069 

Abbreviation Key 

A = Amber 

BT = Bevel Top, Rounded or 

Flat Bottom 

C = Clear Glass 

Gl/Pl = Glass Insert/Plastic Outside 

Gl/Gl = Glass Insert/Glass Outside 

PE = Polyethylene 

PP = Polypropylene 

TPX = Polymethylpentene (PMP) 

Part Numbers are indicated in Bold. 

Unless otherwise indicated, vials are clear glass. 

400 

µL 

30mm 

250 

µL 

29mm 

250 

µL 

31mm 

100 

µL 

30mm 

250 

µL 

29mm 

50 

µL 

25mm 

1.5mL 

1.5mL 

32mm 

1.5mL 

32mm 

1.5mL 

32mm 

C-95160 

A-95163 

C-98030 

A-98095 

12mm 

C-98213 

A-98245 

12mm 

C-98133 

A-98135 

12mm 

6mm 

981391 

PP-99183 

6mm 

C-98322 

PP-98106 

6mm 

C-98745 

PP-98729 

5mm 

C-98052 

PP-92102 

6mm 

C-98049 

PP-98108 

6mm 

98024 

12x32mm Robotic Autosampler Vials 

12x32mm Conical Vials 

1.5mL 

100µL 

32mm 

1.5mL 

1.1mL 

32mm 

1.1mL 

32mm 

1.1mL 

32mm 

C-95386 

A-95388 

PP-90273 

72013 

R.A.M. 

12mm 

R.A.M. 

R.A.M. 

12mm 

12mm 

12mm 

74125 74145 

74135 

12x32mm Limited Volume Vials (One Piece, No Assembly) 

vials 

100µL 

C-95183 

32mm 

1.5mL 300 

µL 

C-72016 

32mm 

100µL 

PP-12960 

TPX-12968 

32mm 

300 

µL 

Gl/Gl- 

98086 

32mm 

200µL 

Gl/Pl- 

98230 

32mm 

.5mL 

PP-98842 

TPX-72011 

32mm 

750µL 

PP-98050 

TPX-72015 

32mm 

0.5mL 

PP-98310 

32mm 

0.7mL 

PP-98099 

32mm 

12mm 

12mm 

12mm 

12mm 

12mm 

12mm 

12mm 

12mm 

12mm 

100µL 

C-9480 

32mm 

300µL 

PP-12990 

300 

µL 

C-95240 

A-95268 

32mm 

0.5mL 

PP-12964 

32mm 

1.5mL 300 

µL 

C-72009 

32mm 

100µL 

C-9482 

32mm 

100µL 

PP-12962 

TPX12970 

32mm 

200µL 

Gl/Pl- 

98220 

32mm 

0.5mL 

PP-12966 

32mm 

12mm 

12mm 

12mm 

12mm 

12mm 

12mm 

12mm 

12mm 

12mm 



15x45mm 4mL WISP Style Vials and Inserts 

4mL 

4 mm 

300 

µL 

45mm 

2mL 

45mm 

4mL 

45mm 

300 

µL 

45mm 

2mL 

45mm 

3 mL 

300 

mL 

38mm 

350 

µL 

42mm 

300 

µL 

38mm 

300 

µL 

38mm 

C-98110 

A-98120 

mm 

98251 

Gl/Pl 

15mm 

PP-98091 

15mm 

C-98040 

A-98097 

15mm 

98154 

Gl/Pl 

15mm 

PP-98054 

15mm 

PP-98972 

6mm 

C-98118 

PP-98071 

8mm 

98942 

PP99165 

6mm 

C-98056 

PP-92104 

6mm 

C-98199 

PP-98193 

8mm Crimp Top 

8x40mm 1mL WISP Style 

Misc. 

98226 

500 

µL 

C-98819 

A-98757 

8mm 

C-98148 

A-98894 

700 

µL 

40mm 

30mm 

500 

µL 

A-98183 

8mm 

800 

µL 

30mm 

C-98043 

A-98896 

40mm 

200 

µL 

94450 

6mm 

98403 

700 

µL 

32mm 

200 

µL 

94470 

32mm 

6mm 

32mm 

98401 

500 

µL 

900µL 

C-98226 

Sleeve- 

98217 

10mm 

32mm 

32mm 

1mL 

8mm 

98494 

800 

µL 

40mm 

C-98210 

A-98214 

PP-98825 

40mm 

700 

µL 

PP-98990 

98799 

1mL 

40mm 

100 

µL 

5mm 

100 

µL 

36mm 

PP99175 

98900 

PP99185 

98227 

36mm 

98273 

2mL 

12mm 

C-50303400 

A-98921 

1mL 

35mm 

40mm 

800µL 

10mm 

98821 

4mL 

65mm 

32mm 

5mL 

98187 

96mm 

8mL 

98250 

100mm 

7mm 7mm 7mm 

Headspace Vials 

BT-99136 

7mm 

8mm 

8mm 

5mm 

8mm 

13mm 

12mm 

13mm 

75mm 

23mm 

20mL 

23mm 

BT-98792 

BT-99132 23mm 

10mL 

10mL 

6mL 

22mm 

38mm 

46mm 

C-6636 

Long Neck 

Flat Bottom 

C-99097 

Spec. Crimp 

SPME 

C-97127 

For PE 

C-97188 

Long Neck 

Round Bottom 

C-BT98788 

A-BT99077 for PE 

Bevel Top 

Round Bottom 

75.5mm 

22.5mm 

C-99065 

A-99068 

46mm 

22.5mm 

C-99070 

A-99078 

For CTC PAL 

65mm 

18mm 

98104 

12mL 

98098 

9mL 

18mm 

50mm 

98093 

27mL 

30mm 

56mm 

vials 


351


vials 

Vial Selection by 

Autosampler 

12x32 Screw 

Thread, 8/425, 

12x32 Crimp Style 

Autosampler Chart 

Autosampler Manufacturer Model 

Agilent 1100, 1200 

• • 

8042, 1050, 1084, 1080 

• 

• 

7670A, 7671A, 7672, 1042 

• 

1090 (LC), 1082, 7673A/B, 

• 

Headspace 

7673, 7683 

• 

• • 

Alcott 738 

• • • • 

718AL, 719D 

• • • • • 

Alltech (Grace) 570, 580 (standard tray) • • • • 

Beckman 501, 502, 507 

• 

• • 

• 

504 

• 

508 

• • • • 

Carlo Erba/Fisons AS-V42, AS 105 

• 

• 

Instruments 

AS-V 60 

AS 800, 8000 

• • 

• 

• 

A200S 

• 

HS250 

Dynatech/Precision 42 Vial Tray, LC 2000 

• • • • 

Sampling 

60 Vial Tray 

GC111, 311 

• • 

• 

• 

• • 

Finnigan A200S • • • 

Gilson 232-401, 231-401 

• • 

• 

• • 

Asted 

• • 

• 

Aspec 

• • 

• 

Hitachi** 

AS-2000, AS-6000 

• • 

• 

S6551 

• 

L-7200, 7250 

• 

• • • 

Jasco** 

851-AS, AS-950 

LC 800,900 Series 

AS-2055/2057/2059 

Leap Technologies CTC A200S 

CTC A105S 

Headspace 

LDC 

Marathon, Promis 

713 

Others 

PerkinElmer 

Autosystem 

Integral 4000 

AI-1, ISS100, ISS200 

LC600 42 Tray 

LC600 (60), AS100 

420/B, 4900 

AS100B, AS300, 8300 

AS2000, LC30, 2000, B2000 

Headspace, HS40 

Series 200 

Pharmacia LKB 2157-010 

2157-020 

Phillips/Pye UNICAM LC-XP 

S4, S8, 4700LC/GC 

4247, 4710 

Shimadzu** 

AOC-8B/9, SIL-6A 

AOC-14/1400, AOC-17 

SIL-2AS 

SIL-6B, -9A, -8A, -10A 

Headspace HSS-2B 

AOC-20i/20S 

AOC-5000 

LC-10A 

SIL-Hta/SIL-HTc 

Spark Holland SPH 125 

Marathon 

Promis 

Triathlon, Midas 

Spectra-Physics/TSP 8875, 8880 

AS100/1000;300/3000 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

12x32 Screw Thread, 

10/425 

• 

12x32 Screw Thread, 

Robotic 

15x45, Screw Thread, 

13/425 

15x45, Crimp Style 





• • 

• • • • • 

• 

• 

• 

• 

• 

• 

• 

• 

• • 

• • 

• • • 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• • 

• • • • • 

• 

• 

• • 

• 

• • 

• 

• • 

• 

• 

• 

• 

Varian Prostar 400, 410, 430 • • • 

8000, 9000 Series • • • • 

Waters Alliance ® 2690 

• • • • 

48-Position 

96-Position 

717 

• 

Alliance ® 

• 

• • 

Acquity UPLC ® 

• 

Breeze 

• • 

• • 

2710 Sample Manager 

• 

• • 

**Special Cap is Required. 

• 

12x32 Snap Seal 

Wide Mouth 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• 

• • • 

• 

• 

• 


Headspace 

• 

• 

• 

• 

• 

• 

Specialty 

• • 

• 

• 

• 

• 

• 

• 



Vials by Autosampler Part No. Cross Reference 

Autosampler Part No. Cross Reference Chart 

Agilent PerkinElmer Varian Waters Grace Description 

12x32mm Standard Mouth Screw Thread Vials 

5183-4428 N930-2945 392611639 — 95191 8/425 Clear Screw Thread Vial 

5183-4429 — 392611640 — 95194 8/425 Amber Screw Thread Vial 

— — — WAT094174 98061 8/425 Black Polypropylene (PP) Open-Hole Cap with PPTFE/Silicone Septum 

5183-4442 N930-3441 392611641 — 980611 8/425 Flush-fit Black PP Open-Hole Cap with PTFE/Silicone Septum 

12x32mm Wide Mouth Screw Thread Vials 

— — 392611646 WAT063300 98133 10/425 Clear Screw Thread Vial 

5182-3454 — — 186000984 72009 High Recovery Vial (HRV), Wide Mouth Crimp 

— — 392611650 WAT094174 98144 10/425 Black PP Open-Hole Cap with PTFE/Silicone Septum 

— — — WAT058876 97013 10/425 Black PP Open-Hole Cap with PTFE/Silicone Septum with Slit 

12x32mm Vials for Use with Robotic Arm, Wide Mouth, Screw Thread Vials 

5182-0714 — 392611653 186000273 95386 R.A.M. Clear Vial for Robotics 

5182-0715 — — — 88540 R.A.M. Clear Vial with Marking Spot for Robotics 

5182-0716 — 392611654 186000848 88542 R.A.M. Amber Vial with Marking Spot for Robotics 

5183-2030 — — — 72013 High Recovery Vial (HRV) for Robotics 

5182-0717 — 392611661 — 95308 9mm Blue Cap with PTFE/Butyl Septum 

5182-0720 — 392611659 186000274 95360 9mm Blue Cap with PTFE/Silicone Septum 

5183-2076 — 392611660 186000305 95385 9mm Blue Cap with PTFE/Silicone Septum with Slit 

12x32mm Standard Mouth Crimp Style Vials 

— N930-1385 392611626 — 66002 12x32mm Clear Standard Mouth Crimp Vial 

— N930-2680 392611628 — 95221 12x32mm Amber Standard Mouth Crimp Vial 

5182-0544 — — WAT094219 98030 12x32mm Clear Snap Ring Vial 

5182-0546 — — — 98033 12x32mm Clear Snap Ring Vial with Marking Spot 

5182-0545 — — WAT094220 98083 12x32mm Amber Snap Ring Vial with Marking Spot 

5182-0550 — — — 98038 11mm Snap TOP Cap with PTFE/Butyl Septum 

5182-0541 — — 186000303 98034 11mm Snap TOP Cap with PTFE/Silicone Septum 

5183-4511 — — 186000304 98168 11mm Snap TOP Cap with PTFE/Silicone Septum with Slit 

— — 392611875 — 95232 11mm Poly Crimp Seal with PTFE/Butyl Septum 

— — 392611873 — 95234 11mm Poly Crimp Seal with PTFE/Silicone Septum 

— — 392611874 — 95325 11mm Poly Crimp Seal with PTFE/Silicone Septum with Slit 

12x32mm Wide Mouth Crimp Style Vials 

5181-3375 — 392611634 WAT094222 98213 12x32mm Clear Wide Mouth Crimp Vial 

5182-0543 — — — 98001 12x32mm Clear Wide Mouth Crimp Vial with Marking Spot 

5181-3376 — 392611635 WAT094223 98003 12x32mm Amber Wide Mouth Crimp Vial with Marking Spot 

11mm Aluminum Seals for 12x32mm Standard and Wide Mouth Crimp Style Vials 

5181-1210 N930-6015 392611632 — 73070 11mm Aluminum Seal with PTFE/Butyl Septum 

5182-0552 — 392611631 — 98740 11mm Aluminum Seal with PTFE/Silicone Septum 

Limited Volume Inserts for 12x32mm Autosampler Vials, i.d. as noted 

5183-2089 N930-2681 392611593 — 95201 100µL LV Glass Insert for Standard Mouth Vial 

5183-2088 — 392611591 — 98031 100µL LV Glass Insert with Polyethylene (PE) Bottom Spring for Std Mouth Vial 

5183-2090 — — — 98028 250µL LV Glass Flat bottom Insert for Standard Mouth Vial 

5183-2085 — 392611596 — 98322 250µL LV Glass Insert for Wide Mouth Vial 

5181-1270 — 392611594 WAT094170 98049 250µL LV Glass Insert with PE Bottom Spring for Wide Mouth Vial 

5181-3377 — — — 981391 400µL LV Glass Flat Bottom Insert for Wide Mouth Vial 

5182-0549 — — — 98108 250µL LV PP Insert with PE Bottom Spring for Wide Mouth Vial 

5183-2087 — — — 99183 350µL LV PP Flat Bottom Insert for Wide Mouth Vial 

15x45mm (4mL) Vials 

5183-4448 — — 186000840 98110 15x45mm Clear Glass Vial 

5183-4450 — — 186001135 98120 15x45mm Amber Glass Vial 

5183-4464 — — 186000841 98610 13/425 Black PP Open-Hole Cap with PTFE/Silicone Septum 

— — — 186000842 97017 13/425 Black PP Open-Hole Cap with PTFE/Silicone Septum with Slit 

— — — WAT015199 98118 300µL Glass Limited Volume Insert, 6x38mm 

8x40mm (1mL) WISP Style Vials 

— — — WAT078515 88613 8mm PE Starburst Snap Plugs, Clear 

Note: This list contains some of the more popular products used in these instruments. Every effort has been made to provide the Grace equivalent product or one most similar to the 

manufacturers’ product description. There are many more Grace ® vial, cap, liner, and kit combinations available on the individual product page listings. 

vials 
















353


8x40mm (1mL) Shell Vials 

• Shell vials with thicker walls for safer sample handling 

• 8x40mm vials for Waters ® WISP 96 style autosamplers 

8x40mm Shell Vials for WISP 96 Style 

100/pk 

Part No. 

1000/pk 

Part No. 

Description 

Shell Vials, 1mL, 8x40mm, Use 8mm Snap Plugs 

Clear Shell Vial 88610 98212 

Amber Shell Vial 88614 98216 

Polypropylene Shell Vial 88625 98827 

Silanized, Clear Shell Vial 886101 — 

Limited Volume Shell Vial, 8mm, Use Polyethylene Conical Snap Plugs 

8x40mm Polypropylene Vial, 700µL 98990 98992 

8x40 Shell Vial Kits are Ideal for Waters ® or 

Shimadzu ® Autosamplers 

Convenience kits simplify ordering with one part number to 

remember. Kits provide both vials and caps for a perfect fit 

every time. 

4718 

best 

value 

8x40mm Vial Kits 

100/pk 

Part No. 

1000/pk 

Part No. 

Description 

8x40mm Shell Vial Kits for WISP Style Autosamplers 

Clear Vials, Clear Snap Plugs with Starburst † 88617 73021 

Amber Vials, Clear Snap Plugs with Starburst † 88597 73003 

Polypropylene Vials, Clear Snap Plugs with 

88626 98224 

Starburst † 

Clear Silanized Vials, Clear Snap Plugs 982108 — 

8x40mm Shell Vial Kits for Shimadzu ® Autosamplers 

Clear Vials, Clear Easy Pierce Snap Plugs with 

Starburst † 

Amber Vials, Clear Easy Pierce Snap Plugs with 

Starburst* 

*Use to prevent bent needles. 

† 

Thinner starburst for use with Shimadzu ® autosamplers. 

70118 70132 

70134 70136 

vials 

Limited Volume Inserts for 8x40 Shell Vials 

• Select the appropriate limited volume insert for your 

microsampling needs 

• Polyethylene springs act as shock adsorbers and help 

maintain proper insert alignment 

6308 

4741 

Limited Volume Inserts for 8x40mm Shell Vials 

100/pk 

Part No. 

1000/pk 

Part No. 

Description 

Glass 

150µL, 5x29mm, Requires Spring 95201 95202 

150µL, with Bottom Spring, 5x29mm 73054 73074 

200µL, 5x36mm 98900 98902 



200µL, 5x31mm 73024 73025 

Metal Springs for Inserts 73026 73027 

200µL, Silanized, No Spring 989008 — 

Polypropylene 

250µL, 6x31mm, No Spring 98106 98406 



Polyethylene Snap Plugs with Starburst † 8mm 

100/pk 

Part No. 

1000/pk 

Part No. 

Description 

Easy Pierce Snap Plug † 

Clear Plug 70117 70129 

Snap Plug with Starburst* 

Clear Plug 88613 98865 

Red Plug 88618 98228 

Blue Plug 88615 98232 

Green Plug 88622 98234 

Yellow Plug 88624 98879 

Silicone Lined Snap Plug, Clear, Resealable 98662 98664 

Conical Snap Plug for Limited Volume Polyethylene Vial 

Clear Plug 98994 98996 

Red Plug 99003 99013 

Blue Plug 99005 99015 

Green Plug 99007 99017 


*Use to prevent bent needles. 

† 

Thinner starburst for use with Shimadzu ® autosamplers. 



12x32mm R.A.M. Robotic Screw Thread Vials 

• Works on virtually every autosampler 

• Use to replace crimp vials for use with robotic arms 

on autosamplers 

• Crimp style height with screw thread configuration 

• Incorporates unique StepVial design to precisely 

align insert and vial 

• Economical kits simplify ordering 

• Choose pre-slit septa for Waters ® Acquity ® Systems 

Silanized 

With White 

Marking Spot 

With White Graduated 

Marking Spots 

100/pk 1000/pk 100/pk 100/pk 1000/pk 100/pk 1000/pk 

Vials 

Clear 95386 95387 953861 88540 88544 95521 95522 

Amber 95388 95389 953881 88542 88546 95531 95532 

Limited Volume Vials 

1.5mL High Recovery Vials 72013 — — — — — — 

100µL Polypropylene Vials 90273 90271 — — — — — 

100µL PMP (TPX) Vials 95096 95098 — — — — — 

Kits: Vials Open-Hole Caps with Septa 

Clear Vials 9mm PTFE/Butyl Septa 89135 89136 — 89120 89126 — — 

Amber Vials 9mm PTFE/Butyl Septa 98004 98006 — 89125 89127 — — 

Clear Vials 9mm PTFE/Silicone Septa 89145 89146 953868 89100 89104 — — 

Clear Vials 9mm PTFE/Silicone/PTFE Septa 89149 89139 — 89128 89129 — — 

Amber Vials 9mm PTFE/Silicone/PTFE Septa 89151 89152 — 89130 89131 — — 

9mm R.A.M. Open-Hole Caps with Septa 

Blue Cap Bonded Interseal PTFE/Silicone Septa with Slit 70416 70418 — 70416 70418 70416 70418 

Blue Cap Bonded Interseal PTFE/Silicone Septa 70415 70417 — 70415 70417 70415 70417 

Black Cap PTFE/Silicone Septa with Slit 95380 95382 — 95380 95382 95380 95382 

Blue Cap PTFE/Silicone Septa with Slit 95385 95390 — 95385 95390 95385 95390 

Black Cap PTFE/Butyl Septa 95320 95319 — 95320 95319 95320 95319 

Blue Cap PTFE/Butyl Septa 95308 95311 — 95308 95311 95308 95311 

Clear Cap PTFE/Butyl Septa 95352 95348 — 95352 95348 95352 95348 

Green Cap PTFE/Butyl Septa 95346 95344 — 95346 95344 95346 95344 

Red Cap PTFE/Butyl Septa 95356 95354 — 95356 95354 95356 95354 

Yellow Cap PTFE/Butyl Septa 95358 95357 — 95358 95357 95358 95357 


Blue Cap PTFE/Silicone Septa with Slit 95360 95359 — 95360 95359 95360 95359 

Clear Cap PTFE/Silicone Septa with Slit 95365 95364 — 95365 95364 95365 95364 

Green Cap PTFE/Silicone Septa with Slit 95362 95361 — 95362 95361 95362 95361 

Red Cap PTFE/Silicone Septa with Slit 95367 95366 — 95367 95366 95367 95366 

Yellow Cap PTFE/Silicone Septa with Slit 95377 95378 — 95377 95378 95377 95378 

Black Cap PTFE/Silicone/PTFE Septa 95328 95338 — 95328 95338 95328 95338 

Blue Cap PTFE/Silicone/PTFE Septa 95379 95381 — 95379 95381 95379 95381 

Clear Cap PTFE/Silicone/PTFE Septa 95371 95370 — 95371 95370 95371 95370 

Green Cap PTFE/Silicone/PTFE Septa 95369 95368 — 95369 95368 95369 95368 

Red Cap PTFE/Silicone/PTFE Septa 95374 95372 — 95374 95372 95374 95372 

Yellow Cap PTFE/Silicone/PTFE Septa 95376 95375 — 95376 95375 95376 95375 

9mm Unlined, Open-Hole R.A.M. Caps 

Black Cap 90257 90268 — 90257 90268 90257 90268 

Red Cap 90259 90270 — 90259 90270 90259 90270 

Blue Cap 90261 90272 — 90261 90272 90261 90272 

Green Cap 90263 90274 — 90263 90274 90263 90274 

Yellow Cap 90265 90276 — 90265 90276 90265 90276 

Clear Cap 90267 90278 — 90267 90278 90267 90278 

Septa for 9mm R.A.M. Caps 

PTFE/Red Rubber—40mil 88701 88691 — 88701 88691 88701 88691 

PTFE/Silicone—40mil 88705 88695 — 88705 88695 88705 88695 

PTFE/Silicone/PTFE—40mil 88710 88699 — 88710 88699 88710 88699 

4715 

12x32mm R.A.M. Robotic Specifications 

32mm 

12mm 

Neck Style: Screw Thread 

Required Closure: 

9mm R.A.M. Screw Thread Caps 

Wide Mouth Opening: 

9mm o.d., 6mm i.d. 

vials 

more info 

12x32 R.A.M. Vials are compatible with Waters ® 

Alliance ® and Acquity ® Systems, Agilent 1100 & 

1200, and Varian ® 8000 & 9000. Refer to page 352 

for more autosampler compatibility. 


Do you have small samples? 

Choose limited volume vials or see our selection 

of limited volume inserts on page 361. 

4748 


355


vials 

12x32 Screw Thread Standard Mouth Vials 

• Work with a variety of autosamplers that require 

narrow neck vials 


more info 

Compatible with Waters ® Alliance ® , Hitachi, Jasco, 

and Varian ® autosamplers. Refer to page 352 for 

more autosampler compatibility. 

Silanized 

With White 

Marking Spot 


Marking Spots 


Vials 

Clear 95191 95192 951911 98068 98161 95481 95482 

Amber 95194 95195 951941 98078 98171 95491 95492 


1.1mL Glass Tapered Bottom, requires sleeve 74135 98574 — — — — — 

Support Sleeve for 74135 (50/pk) 98558 — — — — — — 

100µL Heavy-Walled Glass with PTFE/Silicone-Lined Cap (12/pk, 10x12/pk) 9482 94823 — — — — — 

600µL Polypropylene Vials (500/pk) 89073 — — — — — — 

100µL Polypropylene Vials 12962 12963 — — — — — 

100µL PMP (TPX) Microvials 12970 12971 — — — — — 

200µL Glass Insert in Clear Plastic Vial 98220 98222 — — — — — 

200µL Glass Insert in Amber Plastic Vial 98225 98899 — — — — — 

Preassembled Kits 

Vials Caps with Septa 

Clear Vials 8/425 Black PTFE Septa—10mil 98533 98535 — — — — — 

Clear Vials 8/425 Black PTFE/Butyl Septa—40mil 98380 98382 — — — — 

Clear Vials 8/425 Black PTFE/Silicone Septa—45mil 95175 95176 — — — — — 

Clear Vials 8/425 Black PTFE/Silicone Septa — — 72666 — — — — 

Amber Vials 8/425 Black PTFE/Silicone Septa—45mil 95179 95181 — — — — — 

Amber Vials 8/425 Black PTFE/Silicone Septa 72670 — — — — — — 

Preassembled Kits Spectra Physics/Varian ® 


Clear Vials Flush-Fit 8/425 Black PTFE/Silicone Septa 895175 895176 — — — — — 

Clear Vials Flush-Fit 8/425 Black PTFE/Butyl Septa 898380 898382 — — — — — 

Clear Vials Flush-Fit 8/425 Black PTFE/Silicone/PTFE Septa 898537 898539 — — — — — 

Amber Vials Flush-Fit 8/425 Black PTFE/Butyl Septa 898385 898387 — — — — — 

Preassembled 8/425 Polypropylene 

Open-Hole Caps 

with Septa 

Black Cap Bonded Interseal PTFE/Silicone Septa 70412 70414 — 70412 70414 70412 70414 

Black Flush-Fit Cap PTFE/Silicone Septa 980611 980611 — 980611 980611 980611 980611 


Black Cap PTFE/Silicone Septa 98061 98061 — 98061 98061 98061 98061 


Black Cap PTFE/Silicone TOP Hat Septa 98065 98065 — 98065 98065 98065 98065 

Yellow Cap PTFE/Silicone TOP Hat Septa 98055 98055 — 98055 98055 98055 98055 

Clear Cap TOP Seal Closure, Molded Poly Septa—10mil 98060 98160 — 98060 98160 98060 98160 

Solid 8/425 Top Caps 

with Septa 

Green Phenolic Cap PTFE Septa 73067 98067 — 73067 98067 73067 98067 

Black Polypropylene Cap PTFE Septa 98063 98163 — 98063 98163 98063 98163 

Black Polypropylene Cap — 98105 98109 — 98105 98109 98105 98109 

Open-Hole Unlined 8/425 Caps 

Black Flush-Fit Side Cap for Spectra-Physics and Varian ® Autosamplers 730441 730442 — 730441 730442 730441 730442 

Black Cap 73044 73059 — 73044 73059 73044 73059 

Red Cap 98072 98172 — 98072 98172 98072 98172 

Blue Cap 98074 98174 — 98074 98174 98074 98174 

Green Cap 98076 98176 — 98076 98176 98076 98176 

White Cap 98084 98184 — 98084 98184 98084 98184 

White Cap for Shimadzu ® , Hitachi, and Jasco Autosamplers 98087 98089 — 98087 98089 98087 98089 

Yellow Cap for Shimadzu ® , Hitachi, and Jasco Autosamplers 73063 73065 — 73063 73065 73063 73065 

Septa for 8/425 Caps 

PTFE/Silicone with Slit—5/40mil 98088 98188 — 98088 98188 98088 98188 

White PTFE—10mil 98094 98194 — 98094 98194 98094 98194 

Red PTFE/White Silicone—5/55mil 73048 73060 — 73048 73060 73048 73060 


PTFE/Silicone—10/90mil 98499 98497 — 98499 98497 98499 98497 

Blue PTFE/White Silicone—5/60mil 98747 98749 — 98747 98749 98747 98749 

PTFE/Silicone/PTFE—2/36/2mil 98081 98181 — 98081 98181 98081 98181 

PTFE/Butyl—5/40mil 98190 98192 — 98190 98192 98190 98192 

Black Fluoroelastomer Polymer—30mil 98026 98047 — 98026 98047 98026 98047 

4684 

12x32mm Screw Thread Std. Mouth Specifications 

32mm 

12mm 



8/425mm Screw Thread Caps 

Standard Mouth Opening: 




12x32mm Screw Thread Wide Mouth Vials 

• 40% larger opening aids in sample 

transfers 

• Larger target area prevents bent needles 

• StepVial design precisely aligns insert 

and vial 


4711 

12x32mm Screw Thread Wide Mouth Specifications 

32mm 

12mm 

Neck Style: Wide Mouth Screw Thread 


10/425mm Screw Thread Caps 



Silanized 

With White 

Marking Spot 


Marking Spots 


Vials 

Clear 98133 98433 981331 98291 98295 95501 95502 

Amber 98135 98435 981351 98622 98624 95511 95512 


500µL Polypropylene Vial 98310 98314 — — — — — 

750µL Polypropylene Vial 98099 98211 — — — — — 

Preassembled Kits: 


Clear Vials Black 10/425 PTFE Septa 98891 98906 — — — — — 

Clear Vials Black 10/425 PTFE/Silicone Septa 98893 98908 981338 — — — — 

Clear Vials Black 10/425 PTFE/Silicone/PTFE Septa 98895 98910 — — — — — 

Clear Vials Black 10/425 PTFE/Butyl Septa 98290 98292 — — — — — 

Amber Vials Black 10/425 PTFE Septa 98274 98277 — — — — — 

Amber Vials Black 10/425 PTFE/Silicone Septa 98281 98284 981358 — — — — 

Amber Vials Black 10/425 PTFE/Butyl Septa 98294 98296 — — — — — 

Unassembled Kits: 


Clear Vials Black 10/425 PTFE/Silicone Septa 97239 97229 — — — — — 

Amber Vials Black 10/425 PTFE/Silicone Septa 97249 97259 — — — — — 

Preassembled 10/425 


with Septa 

Black Cap Bonded Interseal PTFE/Silicone Septa w/Slit 70422 70426 — 70422 70426 70422 70426 

Black Cap Bonded Interseal PTFE/Silicone Septa 70411 70413 70411 70413 70411 70413 


Black Cap PTFE/Silicone Septa 98144 98152 — 98144 98152 98144 98152 


Black Cap PTFE/Red Rubber Septa 97274 97216 — 97274 97216 97274 97216 

Clear Cap TOP Seal Closure, Molded Poly Septa 98405 98407 — 98405 98407 98405 98407 

Solid Polypropylene 10/425 Top Caps with Septa 

Black Cap PTFE Septa 98124 98424 — 98124 98424 98124 98424 

Black Cap 98162 98167 — 98162 98167 98162 98167 

Unlined 10/425 Caps 

Black Cap 98143 98443 — 98143 98443 98143 98443 

Red Cap 98136 98436 — 98136 98436 98136 98436 

Blue Cap 98138 98438 — 98138 98438 98138 98438 

Green Cap 98140 98440 — 98140 98440 98140 98440 

Yellow Cap 98122 98134 — 98122 98134 98122 98134 

White Cap 98126 98426 — 98126 98426 98126 98426 



White PTFE—10mil 98023 98423 — 98023 98423 98023 98423 




PTFE/Butyl Rubber—5/40mil 98029 98429 — 98029 98429 98029 98429 

vials 

more info 

12x32 Screw Thread Wide Mouth Vials are 

compatible with Waters ® Alliance ® , Agilent 1100 & 

1200, Varian ® 8000 & 9000. Refer to page 352 for 

more autosampler compatibility. 


Do you have small samples? 

Choose limited volume vials or see our selection 

of limited volume inserts on page 361. 

4748 


357


12x32mm Crimp Style Standard Mouth Vials 

• Work with most autosamplers 


• For small samples use limited volume 

vials or inserts — see page 361 for 

inserts 

12x32mm Crimp Style Std. Mouth Specifications 

Neck Style: Crimp Top 

32mm 

Recommended Closure: 

11mm Aluminum Caps 

Optional Closures: 

11mm Poly Crimp Seals, p359 

4712 

12mm 

Standard Mouth Opening: 


With White 

Marking Spot 


Marking Spots 

100/pk 1000/pk 100/pk 1000/pk 100/pk 1000/pk 

Vials 

Clear 66002 66003 98073 98191 95461 95462 

Amber 95221 95222 98077 98197 95471 95472 


100µL Glass Heavy Walled (12/pk, 10x12/pk) 9480* 94802* — — — — 

600µL Polypropylene Vial (500/pk) — 88363* — — — — 

300µL Polypropylene Microvial 12990 12991 — — — — 

300µL Polypropylene Microvial (12x31mm) 12982 12983 — — — — 

Kits: Vials Seals with Septa 

Clear Vials 11mm Aluminum Seals PTFE Septa—10mil 98389 98391 — — — — 

Clear Vials 11mm Aluminum Seals PTFE/Silicone Septa 98318 98329 — — — — 

Clear Vials 11mm Aluminum Seals PTFE/Butyl Septa 95161 95162 — — — — 

Amber Vials 11mm Aluminum Seals PTFE/Silicone Septa 98368 98371 — — — — 

Amber Vials 11mm Aluminum Seals PTFE/Butyl Septa 95151 98331 — — — — 

11mm Aluminum Caps 

with Septa 

Silver Cap 1/8" Center Hole with PTFE/Butyl Septa—40mil 98298 98304 98298 98304 98298 98304 

Silver Cap PTFE/Butyl Septa—40mil 73070 73071 73070 73071 73070 73071 

Red Cap PTFE/Butyl Septa—40mil 98051 98151 98051 98151 98051 98151 

Blue Cap PTFE/Butyl Septa—40mil 98053 98153 98053 98153 98053 98153 

Green Cap PTFE/Butyl Septa—40mil 98057 98157 98057 98157 98057 98157 

Gold Cap PTFE/Butyl Septa—40mil 98059 98159 98059 98159 98059 98159 

Silver Cap PTFE/Silicone Septa—3/37mil 98740 94867 98470 94867 98740 94867 

Silver Cap PTFE/Silicone/PTFE Septa—2/36/2mil 94855 94865 94855 94865 94855 94865 

Silver Cap Black Fluoroelastomer Polymer Septa—40mil 94852 94862 94852 94862 94852 94862 

Silver Cap PTFE/Gray Chlorobutyl Septa—2/38mil 98742 94870 98742 94870 98742 94870 

Silver Cap Polypropylene Septa—10mil 98011 98111 98011 98111 98011 98111 

Silver Cap PTFE Septa—10mil 98013 98113 98013 98113 98013 98113 

Unlined 11mm Aluminum Seals 

Silver Seal, Open-Hole 98746 66405 98746 66405 98746 66405 

Septa for 11mm Aluminum Seals 

White PTFE—10mil 98007 98017 98007 98017 98007 98017 

PTFE/Silicone—5/55mil 98009 98019 98009 98019 98009 98019 

PTFE/Silicone/PTFE—2/36/2mil 98010 98020 98010 98020 98010 98020 

PTFE/Butyl Rubber—5/40mil 98398 98400 98398 98400 98398 98400 

*See correct quantity specified in description. 

vials 

more info 

12x32mm Crimp Style Vials (on pages 358, 359) 

are compatible with Agilent and Varian ® 

autosamplers. Refer to page 352 for detailed 

autosampler compatibility. 

4949 


Looking for a crimper or decrimper? 






3309 



12x32mm Crimp Style Wide Mouth Vials 

Standard Crimp Top 

• 40% larger opening aids 

in sample transfers and 

prevents bent needles 

• StepVial design precisely 

aligns insert and vial 


4713 

Snap Seal 

• Unique design 

allows secure seal 

• Economical kits 

simplify ordering 

With White 

Marking Spot 


Marking Spots 

100/pk 1000/pk 100/pk 1000/pk 100/pk 1000/pk 

Snap Seal Vials 

Clear 95160 95190 95165 95155 95401 95402 

Amber 95163 95153 95167 95157 95411 95412 

Crimp Top Vials 

Clear 98213 98239 98001 98201 95441 95442 

Amber 98245 98249 98003 98203 95451 95452 

Snap Seal Limited Volume Vials 

100µL Clear Glass Micro Vial, Heavy Wall 95183 — — — — — 

1.5mL Clear Glass High Recovery (HRV) Vial 72016 — — — — — 

Crimp Top Limited Volume Vials 

1.5mL Glass High Recovery (HRV) Vial 72009 — — — — — 

2mL Glass Round Bottom Vial 74125 98560 — — — — 

1.1mL Glass Tapered Bottom Vial, Requires Sleeve 74145 98562 — — — — 

Support Sleeve for 74145 (50/pk) 98558 — — — — — 

300µL Clear Glass LVI Vial — — 95240 — — — 

300µL Amber Glass LVI Vial — — 95268 — — — 

Snap Seal Kits: 

Vials Seals with Septa 

Clear Vials 11mm Poly Crimp Seals PTFE/Butyl Septa 88487 88491 — — — — 

Clear Vials 11mm Poly Crimp Seals PTFE/Silicone Septa 88496 88475 — — — — 

Clear Vials 11mm Poly Crimp Seals PTFE/Silicone/PTFE Septa 88494 88473 — — — — 

Amber Vials 11mm Poly Crimp Seals PTFE/Butyl Septa 88493 88479 — — — — 

Amber Vials 11mm Poly Crimp Seals PTFE/Silicone Septa 88498 88484 — — — — 

Crimp Top Kits: 

Vials Seals with Septa 

Clear Vials 11mm Aluminum Seals PTFE Septa—10mil 98457 98459 — — — — 

Clear Vials 11mm Aluminum Seals PTFE/Silicone Septa 98419 98428 — — — — 

Clear Vials 11mm Aluminum Seals PTFE/Silicone/PTFE Septa 98447 98451 — — — — 

Clear Vials 11mm Aluminum Seals PTFE/Butyl Septa 98397 98404 — — — — 

Amber Vials 11mm Aluminum Seals PTFE/Silicone Septa 98434 98437 — — — — 

Amber Vials 11mm Aluminum Seals PTFE/Butyl Septa 98413 98417 — — — — 

Clear Vials 11mm Poly Crimp Seals PTFE/Silicone Septa 88369 88366 — — — — 

Clear Vials 11mm Poly Crimp Seals PTFE/Butyl Septa 88368 88380 — — — — 

Amber Vials 11mm Poly Crimp Seals PTFE/Silicone Septa 88375 88378 — — — — 

Amber Vials 11mm Poly Crimp Seals PTFE/Butyl Septa 88374 88396 — — — — 

11mm Poly Crimp Seals 

with Septa 

Clear Seal PTFE/Silicone Septa with Slit 95325 95340 95325 95340 95325 95340 

Clear Seal PTFE/Silicone Septa with Starburst 95335 95350 95335 95350 95335 95350 

Clear Seal PTFE Septa—10mil 95230 95330 95230 95330 95230 95330 

Red Seal PTFE Septa—10mil 95207 95341 95207 95341 95207 95341 

Blue Seal PTFE Septa—10mil 95215 95343 95215 95343 95215 95343 

Green Seal PTFE Septa—10mil 95217 95345 95217 95345 95217 95345 

Yellow Seal PTFE Septa—10mil 95219 95347 95219 95347 95219 95347 

Clear Seal PTFE/Butyl Septa 95232 95332 95232 95332 95232 95332 

Red Seal PTFE/Butyl Septa 95235 95349 95235 95349 95235 95349 

Blue Seal PTFE/Butyl Septa 95237 95351 95237 95351 95237 95351 

Green Seal PTFE/Butyl Septa 95239 95353 95239 95353 95239 95353 

Yellow Seal PTFE/Butyl Septa 95233 95355 95233 95355 95233 95355 

Clear Seal PTFE/Silicone Septa 95234 95334 95234 95334 95234 95334 

Red Seal PTFE/Silicone Septa 95293 95224 95293 95224 95293 95224 

Blue Seal PTFE/Silicone Septa 95295 95225 95295 95225 95295 95225 

Green Seal PTFE/Silicone Septa 95297 95226 95297 95226 95297 95226 

Yellow Seal PTFE/Silicone Septa 95285 95227 95285 95227 95285 95227 

Clear Seal PTFE/Silicone/PTFE Septa 95236 95336 95236 95336 95236 95336 

Red Seal PTFE/Silicone/PTFE Septa 95310 95164 95310 95164 95310 95164 

Blue Seal PTFE/Silicone/PTFE Septa 95312 95166 95312 95166 95312 95166 

Green Seal PTFE/Silicone/PTFE Septa 95316 95182 95316 95182 95316 95182 

Yellow Seal PTFE/Silicone/PTFE Septa 95318 95184 95318 95184 95318 95184 

4712 

12x32mm Crimp Style Wide Mouth Specifications 

32mm 

12mm 

Neck Style: Crimp Top 


11mm Aluminum Caps, p358 



Neck Style: 

Snap Seal 




11mm Snap TOP Caps, p360 


Wide Mouth Opening (for both): 


12mm 

32mm 

vials 


359


12x32mm Crimp Style Wide Mouth Vials 

Snap Ring 

• 40% larger opening aids in sample 

transfers 


and vial 


4714 

12x32mm Crimp Style Wide Mouth Specifications 

32mm 

12mm 

Neck Style: Snap Ring 


11mm Snap TOP Caps 



11mm Poly Crimp Seals, p359 



vials 

With White 

Marking Spot 


Marking Spots 

100/pk 1000/pk 100/pk 1000/pk 100/pk 1000/pk 

Snap Ring Vials 

Clear 98030 98130 98033 98202 95421 95422 

Amber 98095 98195 98083 98205 95431 95432 

Snap Ring Limited Volume Vials 

300µL Glass Insert in Clear Glass Vial 98086 98238 — — — — 

750µL Polypropylene Vial 98050 98215 — — — — 

750µL TPX (PMP) Vial 72015 72017 — — — — 


500µL TPX (PMP) Vial 72011 72014 — — — — 


100µL TPX (PMP) Microvial 12968 12969 — — — — 

200µL Glass Insert in Clear Vial 98230 98231 — — — — 

200µL Glass Insert in Amber Vial 98235 98236 — — — — 

Snap Ring Kits: 


Clear Vials 11mm Snap TOP Caps PTFE/Silicone Septa with Slit 98465 98467 — — — — 

Clear Vials 11mm Snap TOP Caps PTFE/Silicone Septa with Starburst 98469 98471 — — — — 

Clear Vials 11mm Snap TOP Caps PTFE/Butyl Septa 98473 98477 — — — — 

Clear Vials 11mm Snap TOP Caps PTFE/Silicone Septa 98483 98485 — — — — 

Amber Vials 11mm Snap TOP Caps PTFE/Butyl Septa 98479 98481 — — — — 

Amber Vials 11mm Snap TOP Caps PTFE/Silicone Septa 98487 98489 — — — — 


with Septa 

Clear Cap PTFE/Silicone Septa with Slit 98168 98268 98168 98268 98168 98268 

Clear Cap PTFE/Silicone Septa with Starburst 98252 98255 98252 98255 98252 98255 

Clear Cap PTFE/Silicone/PTFE Septa with Starburst 98257 98259 98257 98259 98257 98259 

Clear Cap Red Fluoroelastomer Polymer Septa 98223 98243 98223 98243 98223 98243 

Clear Cap PTFE Septa—10mil 98032 98442 98032 98442 98032 98442 

Red Cap PTFE Septa—10mil 98332 98333 98332 98333 98332 98333 

Blue Cap PTFE Septa—10mil 98334 98335 98334 98335 98334 98335 

Green Cap PTFE Septa—10mil 98336 98337 98336 98337 98336 98337 

Yellow Cap PTFE Septa—10mil 98338 98339 98338 98339 98338 98339 

Clear Cap PTFE/Silicone Septa—10mil 98034 98444 98034 98444 98034 98444 

Red Cap PTFE/Silicone Septa—10mil 98340 98341 98340 98341 98340 98341 

Blue Cap PTFE/Silicone Septa—10mil 98342 98343 98342 98343 98342 98343 

Green Cap PTFE/Silicone Septa—10mil 98344 98345 98344 98345 98344 98345 

Yellow Cap PTFE/Silicone Septa—10mil 98346 98347 98346 98347 98346 98347 

Clear Cap PTFE/Silicone/PTFE Septa—40mil 98036 98446 98036 98446 98036 98446 

Red Cap PTFE/Silicone/PTFE Septa—40mil 98348 98349 98348 98349 98348 98349 

Blue Cap PTFE/Silicone/PTFE Septa—40mil 98350 98351 98350 98351 98350 98351 

Green Cap PTFE/Silicone/PTFE Septa—40mil 98352 98353 98352 98353 98352 98353 

Yellow Cap PTFE/Silicone/PTFE Septa—40mil 98354 98355 98354 98355 98354 98355 

Clear Cap PTFE/Butyl Rubber Septa—40mil 98038 98448 98038 98448 98038 98448 

Red Cap PTFE/Butyl Rubber Septa—40mil 98356 98357 98356 98357 98356 98357 

Blue Cap PTFE/Butyl Rubber Septa—40mil 98358 98359 98358 98359 98358 98359 

Green Cap PTFE/Butyl Rubber Septa—40mil 98362 98363 98362 98363 98362 98363 

Yellow Cap PTFE/Butyl Rubber Septa—40mil 98364 98365 98364 98365 98364 98365 

Clear Cap Polyethylene Septa—10mil 98170 98270 98170 98270 98170 98270 

11mm GC Snap TOP Caps 

with Septa 

Clear Cap PTFE/Red Butyl Septa 88351 88364 88351 88364 88351 88364 

Clear Cap PTFE/Silicone Septa 88359 88367 88359 88367 88359 88367 

Clear Cap PTFE/Silicone/PTFE Septa 88362 88469 88362 88469 88362 88469 

more info 

GC Snap TOP Caps provide better seal 

for volatile samples. 


Looking for crimping tools? 




Limited Volume Inserts for 12x32mm Vials 

• Select the appropriate limited volume insert for your 

microsampling needs 

• Polyethylene springs act as shock adsorbers and 

help to maintain proper insert alignment 

• Flange step inserts align precisely in StepVial 

design neck 

Limited Volume Inserts for Standard Mouth Vials (4mm i.d.) 

Description 

100/pk 

Part No. 

1000/pk 

Part No. 

Glass 

100µL, No Spring 95201 95202 

100µL, with Polyethylene Bottom Spring 98031 98131 


50-100µL, Requires Spring 95203 95204 

Metal Springs for (95203) 95205 95206 

250µL, Flat Bottom 98028 98128 

100µL, Silanized, No Spring Required 952018 — 

Polypropylene 

100µL, No Spring 98137 98237 


250µL, Flat Bottom, 5x31mm 99176 99179 

Glass for Screw Thread 4mm i.d. Only 

100µL, with Polyethylene Top Flange 98107 98117 

4747 

Vial Adapter 

For Use with Waters ® Alliance ® Autosampler 

• Reuseable adapter 

• Adds versatility to use of your Waters ® 717 and Alliance ® 

autosamplers 

Limited Volume Inserts for Wide Mouth Vials (6mm i.d.) 

Description 

100/pk 

Part No. 

1000/pk 

Part No. 

Glass 

250µL, No Spring 98322 98324 

250µL, Glass Flange Step Insert 98745 98755 


100µL, with Polyethylene Top Spring 98052 98452 


400µL, Flat Bottom 981391 98439 

250µL, Silanized 983228 — 

250µL, Silanized with Polyethylene Bottom Spring 99060 — 

Polypropylene 

100µL, with Polyethylene Top Spring 92102 98402 


250µL, No Spring Required 98106 98406 

250µL, Polyethylene Flange Step Insert 98729 98739 

350µL, Flat Bottom, 6x30mm 99183 99189 

Prepare samples in one 8x30mm vial and analyze using 

either the Waters ® WISP 96 or Alliance ® autosampler trays. 





5222 

Vial Adapter for Waters ® Alliance ® Autosampler 

Description 

100/pk 

Part No. 

1000/pk 

Part No. 

Reuseable Adapter for Alliance ® 12x32mm Autosampler Tray 

Adapter for Use of 8x30mm Vials in a 12x32mm 98374* — 

Tray, 24/pkg 

Kits —Vials and Caps for Use with Waters ® 717 or Alliance ® Autosamplers 

8x30mm Shell Vials, 8mm Clear Starburst 

98100 98360 

Snap Plugs 

Vials and Caps for Use with Waters ® 717 or Alliance ® Autosamplers 

8x30mm Shell Vials, Clear Glass 97029 98037 

8x30mm Polypropylene Shell Vial, 900µL 99184 99192 

8x30mm Polypropylene LV Shell Vial, 500µL 99178 99182 

8mm Clear Starburst Snap Plugs 88613 98865 

Vials and 10/425 Caps for Waters ® Alliance ® Autosamplers 

12x32mm Clear Wide Mouth Vials 98133 98433 

12x32mm Amber Wide Mouth Vials 98135 98435 

Black Polypropylene Caps, PTFE/Silicone Septum, 98144 98152 

60mil 

*Correct quantity specified in description. 

vials 


361


vials 

15x45mm Screw Thread Vials 

• Compatible with Waters ® WISP 

48 position, 717, Breeze and 

Shimadzu ® autosamplers 

• Use for waste/wash vials for other 

autosamplers 

• 13/425 open top caps and septa 


and vial 


Silanized 

With White 

Marking Spot 


Marking Spots 


Vials 

Clear 98110 98207 981101 98241 98247 97084 97088 

Amber 98120 98484 981201 98261 98267 97097 97117 


3.1mL Glass High Recovery Vial 72018 — — — — — — 

1.2mL Polypropylene Limited Volume Vial 70149 70198 — — — — — 

2.5mL Polypropylene Vial 98091 98491 — — — — — 

300µL Plastic Vial with Glass Insert 98251 98253 — — — — — 

Preassembled Kits: 


Clear Vials Open-Hole Caps PTFE Septa—10mil 98000 98002 — — — — — 

Amber Vials Open-Hole Caps PTFE Septa—10mil 98004 98006 — — — — — 

Clear Vials Open-Hole Caps PTFE/Silicone Septa—10/50mil 98008 98012 72676 — — — — 

Amber Vials Open-Hole Caps PTFE/Silicone Septa—10/50mil 98014 98016 72680 — — — — 

Clear Vials Open-Hole Caps PTFE/Silicone/PTFE Septa 98549 98551 — — — — — 

Amber Vials Open-Hole Caps PTFE/Silicone/PTFE Septa 98618 98621 — — — — — 

Clear Vials Open-Hole Caps PTFE/Butyl Septa—40mil 98623 98626 — — — — — 

Amber Vials Open-Hole Caps PTFE/Butyl Septa—40mil 98628 98630 — — — — — 

Clear Vials Open-Hole Caps TOP Hat Septa—65mil 98633 98632 — — — — — 


250µL Flange Step Glass Insert 70205 70215 — 70205 70215 70205 70215 

275µL 7x44mm Polypropylene LV Insert 99165 99180 — 99165 99180 99165 99180 

300µL 6x38mm Glass Insert, Requires Metal Spring 98118 98218 — 98118 98218 98118 98218 

300µL 6x38mm Polypropylene Insert, Requires Metal Spring 98071 98271 — 98071 98271 98071 98271 

Metal Springs for 300µL Inserts 98121 98221 — 98121 98221 98121 98221 

300µL Self Centering Glass Top Spring 98056 98256 — 98056 98256 98056 98256 

300µL Self Centering Polyethylene Top Spring 92104 98204 — 92104 98204 92104 98204 

300µL Self Centering Glass Bottom Spring 98199 98299 — 98199 98299 98199 98299 

300µL Self Centering Polyethylene Bottom Spring 98193 98293 — 98193 98293 98193 98293 

300µL Silanized Glass Insert — — 981188 — — — — 

350µL Flange Step Glass Insert 98942 98944 — 98942 98944 98942 98944 

500µL 8x43mm Glass Flat Bottom Insert 98581 98583 — 98581 98583 98581 98583 

Preassembled 13/425 


with Septa 

Black Cap Bonded Interseal PTFE/Silicone Septa 70420 70424 — 70420 70424 70420 70424 

Black Cap PTFE/Silicone Septa with Slit—60mil 97017 97027 — 97017 97027 97017 97027 

Black Cap PTFE/Silicone Septa—65mil 98610 98612 — 98610 98612 98610 98612 


Black Cap PTFE/Silicone TOP Hat Septa 98075 98475 — 98075 98475 98075 98475 

Clear Cap TOP Seal Closure with Molded Septa—10mil 98070 98480 — 98070 98480 98070 98480 

Solid 13/425 TOP Caps with Septa 

Solid Green Phenolic Cap with PTFE Septa 98209 98476 — 98209 98476 98209 98476 

Solid White Cap with Interseal PTFE/Silicone Bonded Septa 99031 — — 99031 — 99031 — 

Solid Black Cap with PTFE/F217 Septa 98430 98432 — 98430 98432 98430 98432 

Solid Black Cap 98614 98616 — 98614 98616 98614 98616 

Unlined 13/425 Caps 

Black Cap 73090 73092 — 73090 73092 73090 73092 

Red Cap 98454 98466 — 98454 98466 98454 98466 

Blue Cap 98456 98468 — 98456 98468 98456 98468 

Green Cap 98458 98470 — 98458 98470 98458 98470 

Yellow Cap 98460 98472 — 98460 98472 98460 98472 

White Cap 98462 98474 — 98462 98474 98462 98474 


PTFE—10mil 73094 73096 — 73094 73096 73094 73096 


PTFE/Butyl Rubber—5/55mil 98367 98369 — 98367 98369 98367 98369 

Red PTFE/Silicone—5/55mil 98846 98848 — 98846 98848 98846 98848 

PTFE/White Silicone—10/50mil 98123 98450 — 98123 98450 98123 98450 

PTFE/White Silicone—10/90mil 98219 95248 — 98219 95248 98219 95248 


Black Fluoroelastomer Polymer—30mil 98606 98608 — 98606 98608 98606 98608 

4716 

15x45mm Screw Thread Specifications 

45mm 

15mm 



13/425 Screw Thread Caps 



15x45mm Crimp Style Vials 

• Compatible with Waters ® WISP 

48 position, 717 and Shimadzu ® 

autosamplers 

• Use for waste/wash vials for other 

autosamplers 


15x45mm Crimp Style Vial Specifications 

45mm 

Neck Style: 

Snap Ring 

Neck Style: 

Snap Seal 



13mm Aluminum Seals 

45mm 


Marking Spots 

100/pk 1000/pk 100/pk 1000/pk 

Snap Seal Vials, 4mL 

Clear 98040 98240 95545 95546 

Amber 98097 98297 95547 95548 

Snap Ring Limited Volume Vials 

300µL Plastic Vial with Glass Insert 98154 98156 — — 

2.5mL Polypropylene Vial 98054 98254 — — 


Snap Seal Vials Caps with Septa 

Clear Vials 13mm Snap TOP Caps PTFE Septa—10mil 98390 98392 — — 

Clear Vials 13mm Snap TOP Caps PTFE/Silicone Septa 98874 98876 — — 

Amber Vials 13mm Snap TOP Caps PTFE/Silicone Septa 98870 98872 — — 

Clear Vials 13mm Snap TOP Caps PTFE/Butyl Septa 98636 98638 — — 

Amber Vials 13mm Snap TOP Caps PTFE/Butyl Septa 98640 98644 — — 

Clear Vials 13mm Snap TOP Caps PTFE/Silicone/PTFE Septa 98646 98648 — — 


Snap Seal Vials Seals with Septa 

Clear Vials Caps PTFE/Silicone Septa 98132 98269 — — 

Clear Vials Caps PTFE/Butyl Septa 98145 97266 — — 

Clear Vials Caps PTFE/Silicone/PTFE Septa 98147 97273 — — 


250µL Flange Step Glass Insert 70205 70215 70205 70215 

275µL 7x44mm Polypropylene LV Insert 99165 99180 99165 99180 

300µL 6x38mm Glass Insert, Requires Metal Spring 98118 98218 98118 98218 

300µL 6x38mm Polypropylene Insert, Requires Metal Spring 98071 98271 98071 98271 

Metal Springs for 300µL Inserts 98121 98221 98121 98221 

300µL Self Centering Glass Top Spring 98056 98256 98056 98256 

300µL Self Centering Polyethylene Top Spring 92104 98204 92104 98204 

300µL Self Centering Glass Bottom Spring 98199 98299 98199 98299 

300µL Self Centering Polyethylene Bottom Spring 98193 98293 98193 98293 

350µL Flange Step Glass Insert 98942 98944 98942 98944 

500µL 8x43mm Glass Flat Bottom Insert 98581 98583 98581 98583 


with Septa 

Clear Cap PTFE Septa—10mil 98042 98242 98042 98242 

Clear Cap PTFE/Silicone Septa 98044 98244 98044 98244 

Clear Cap PTFE/Silicone/PTFE Septa 98046 98246 98046 98246 

Clear Cap PTFE/Butyl Rubber Septa 98048 98248 98048 98248 

Clear Cap PTFE/Silicone Septa with Starburst 98586 98588 98586 98588 

Clear Cap PTFE/Silicone/PTFE Septa with Starburst 98593 98594 98593 98594 

13mm Aluminum Seals 

with Septa 

Silver Seal PTFE Septa—10mil 98101 98103 98101 98103 

Silver Seal PTFE/Silicone Septa 98283 98289 98283 98289 

Silver Seal White PTFE/Butyl Septa 98776 98206 98776 98206 

Silver Seal PTFE/Silicone/PTFE Septa 98596 98598 98596 98598 

Gold Seal Pharma-Fix Septa 6687 98208 6687 98208 

13mm Aluminum Seals, Unlined 

Silver Seal, Center Tear Off 98778 66410 98778 66410 

Red Seal, Center Tear Off 98780 — 98780 — 

Blue Seal, Center Tear Off 98783 — 98783 — 

Green Seal, Center Tear Off 98785 — 98785 — 

Gold Seal, Center Tear Off 98787 — 98787 — 

Silver Seal, Tear-Away 98789 66416 98789 66416 

13mm Septa for Aluminum Seals 

White PTFE—10mil 98177 — 98177 — 

PTFE/Silicone—10/50mil 98791 95253 98791 95253 

PTFE/Silicone—10/90mil 98793 95254 98793 95254 

PTFE/Silicone/PTFE—2/36/2mil 98180 98182 98180 98182 

PTFE/Butyl Rubber—40mil 98173 98175 98173 98175 

4717 

15mm 

15mm 

vials 


363



• Crimp style vials, made of 

borosilicate glass 

• New screw thread headspace vial 

• Five finish styles available 

• Choose from three vial sizes, 

6, 10, or 20mL 

For reproducible results, it’s very important 

that the headspace vials and caps form a 

tight seal. In headspace analysis, the sample 

is sealed into the vial and heated until the 

concentration in the gas phase (headspace) 

is in equilibrium with the concentration in the 

liquid phase. An aliquot from the headspace 

is then analyzed. 

Shimadzu ® HSS-2B 

30x60mm 

27mL 

4726 

Vials for Tekmar ® 

4725 

Flat Top, 

Flat Bottom 

20mL 


4725 

Flat Top, 

Round Bottom, 

Long Neck, 20mL 

4725 

Bevel Top, 

Flat Bottom 

20mL 

4725 

Bevel Top, 

Round Bottom 

20mL 

100/pk 

Part No. 

1000/pk 

Part No. 

Description 

Flat Top, Flat Bottom, Clear Glass, Long Neck 

22.5x75.5mm, 20mL Vials 6636 66361 

22.5x46mm, 10mL Vials 6655 66551 

Flat Top, Round Bottom, Clear Glass 

22.5x75.5mm, 20mL Vials for CTC PAL, Long Neck 97188 97190 

22.5x75.5mm, 20mL Vials, for SPME** 99097 99099 

Bevel Top, Flat Bottom, Clear Glass 

23x75mm, 20mL Vials 99136 99138 

23x46mm, 10mL Vials 98792 98794 

22x38mm, 6mL Vials 99132 99134 

Bevel Top, Round Bottom 

23x75.5mm, 20mL Vials, Clear 98788 98790 

23x75.5mm, 20mL Vials, Amber 99077 99093 

22.5x46mm, 10mL Vials, Clear 98938 98676 

22x38.25mm, 5mL Vials, Clear 99056 99058 

Shimadzu ® Headspace Vials 

30x60mm, 27mL, Clear Vials 98093 98546 

Tekmar ® Headspace Vials 

18x50mm, 9mL, Clear Vials, 125/pk 98098* 98548 

18x65mm, 12mL, Clear Vials, 125/pk 98104* 98877 

23x75.5mm, 20mL, Clear Vials 95128 95137 

Screw Thread Headspace Vials for CTC PAL*** 

20mL Clear 99065 99069 

20mL Amber 99068 99074 

10mL Clear 99070 99072 

10mL Amber 99078 99075 

*Correct quantity specified in description. 

**SPME vial has thicker glass top so cap with thinner septum will crimp tightly. 

***New units only. Older models have weak magnets. 

6755 

Screw Top, 

Flat Bottom, 

20mL 

Cross Reference of Headspace Vials to Autosamplers 

Instrument manufacturers recommend use of various vial shapes for best 

results with their analytical systems so we offer all of the vial shapes available. 

vials 

18x50mm 

9mL 

18x65mm 

12mL 

4727 

4725 

Bevel Top, 

Round Bottom 

20mL 

Headspace Vials** and Corresponding Autosamplers 

Recommended For Autosampler Part No. Vial Description 

PerkinElmer ® HS6 99056 5mL Bevel Top/Bound Bottom 

Carlo Erba HS250/HS800; CTC500; 

98938 10mL Bevel Top/Round Bottom 

Fisons HS500/HS800; Chrompack 9020/25 

PerkinElmer ® HS40/HS100/101 98788 20mL Bevel Top/Round Bottom 

Tekmar ® 7000 Series 95128 20mL Bevel Top/Round Bottom 

CTC PAL 97188 20mL Flat Top/Round Bottom 

Carlo Erba HS850; Dani HS86.50/HS39.5; 6655,6636 10mL, 20mL Flat Top/Flat Bottom 

Fisons HS850; Hewlett-Packard HS 7694 

SPME 99097 20mL, Magnetic Cap 

Shimadzu ® 98093 27mL, 30x60mm 

CTC PAL, Gerstel 99065/99070 

99065/99070 

Screw Thread Magnetic Cap 

Screw Thread Magnetic Cap 

**This list is only a guide to the most popular vial and autosampler combinations. Many instruments will 

allow use of other combinations of flat or beveled tops with flat or rounded bottoms. If a particular type of 

vial is required for your instrument, the information should be provided by your autosampler or instrument 

manufacturer. 



20mm Seals and Septa for Headspace Vials 

• Increased selection of septa in aluminum, pressure release (PR), or magnetic seals 

• Now available in convenience kits with headspace vials and pressure release caps 

20mm Polyethylene Seal 

Low cost, polyethylene seal for 

rinse vials or low pressure analysis. 

No crimping is required. 

Types of Aluminum Seals 

Center Hole 

Center Tear-Off 

Magnetic Caps 

Types of Septa 

4998 

4999 5001 

5002 5003 

5000 

5005 

Aluminum Faced/Silicone 

Stoppers 

5mm Center Hole 

5007 

PTFE Faced/Butyl Molded Septum 

(Pharma-Fix Septum) 

Pressure Release (PR) 

Tear-Away 

4999 

8mm Center Hole 

5006 

Silicone or PTFE/Silicone 

20mm Seals and Septa for Headspace Vials 


Polyethylene Seal 

with PTFE/Silicone Septum—5/45mil 100 95586 1000 95576 

Aluminum Seals, Open Center Hole with Septa 

Seal, PTFE/Butyl Rubber Septum 100 98801 500 98552 

Seal, PTFE/Silicone Septum— 100 95756 1000 95758 

10/50mil 

Seal, PTFE/Silicone Septum—125mil 100 95587 1000 95577 

Seal, PTFE/Chlorobutyl Septum 100 95584 1000 95578 

Seal, Aluminum/Silicone Septum 100 98687 1000 98689 

Seal, Black Butyl Rubber Septum 100 98683 1000 98685 

Center Tear-Off Seal, Butyl Septum 72 98841 10x72 98550 

Specialty Seals with Septa 

Seal with Pharma-Fix Septum 100 6689 1000 98898 

PR Seal, PTFE/Silicone Septum— 100 95581 1000 95579 

118mil 

PR Seal, PTFE/Gray Butyl Septum— 100 95590 1000 95580 

118mil 

PR Seal, Aluminum/Silicone 

100 98691 1000 98693 

Septum—118mil 

Magnetic*, 5mm Center, PTFE/ 100 95594 1000 95588 

Silicone Septum—118mil 

Magnetic*, 8mm Center, PTFE/ 100 95139 1000 95143 

Silicone Septum—118mil, 

Magnetic*, 5mm Center, PTFE/Gray 100 95592 1000 95582 

Butyl Septum—118mil 

Magnetic*, 8mm Center, PTFE/Gray 100 95156 1000 95189 

Butyl Septum—118mil 

Magnetic Seal for SPME Vial, PTFE/ 100 99102 1000 99106 

Silicone Septum 1.5mm 

Magnetic Seal for SPME, 

100 99104 1000 99107 

Fluoroelastomer Polymer Septum, 

1.0mm 

Aluminum Seals without Septa 

Standard Seals, 10mm Center Hole 144 66381 1000 89075 

Tear-Away Seals, Silver 144 66501 10x144 98805 

Silver, Center Tear-Off Seal 144 66440 10x144 98803 

Red, Center Tear-Off Seal 144 66441 10x144 66418 

Blue, Center Tear-Off Seal 144 66442 10x144 66419 

Green, Center Tear-Off Seal 144 66443 10x144 66425 

Gold, Center Tear-Off Seal 144 66444 10x144 66426 

Magnetic Tinplate Seal*, 5mm Center 100 98532 1000 98534 

Septa for 20mm Aluminum Seals 

Red Butyl Septum 100 98695 1000 98697 

Red Silicone Septum 72 98536 10x72 98538 

PTFE/Silicone Septum—10/90mil 72 95313 10x72 98809 

PTFE/Gray Butyl Septum 72 98540 10x72 98542 

Aluminum Faced/Silicone—60mil 72 98665 10x72 98667 

Stoppers for 20mm Crimp Top Vials 

Natural Rubber Stoppers 72 95304 1000 95255 

Butyl Rubber Stoppers 72 95305 1000 95256 

Hycar Rubber Stoppers 72 6683 10x72 98813 

Magnetic Screw Thread Caps with Septa for Screw Thread Headspace Vials 

White PTFE/Blue Silicone Septum, 100 99071 1000 99081 

1.3mm 

PTFE/ Red Butyl Septum, 1.6mm 100 99073 1000 99083 

Blue PTFE/White Silicone Septum, 100 99076 1000 99091 

1.5mm 

Fluoroelastomer Polymer, 1.0mm 100 99079 1000 99086 

*Magnetic Seals are used with CTC and Carlo Erba autosamplers. 

vials 

4766 


365


Specialty Autosampler Vials 

8x40mm 

7x32mm 

4718 

Shimadzu ® 

ASI-5000 

4722 

8x30mm 

7x40mm 

DuPont ® 

Vials 

4719 

4720 

Specialty Autosampler Vials 


8x40mm WISP Crimp Style Vials (Use 8mm Closures Below) 

1.0mL Clear Vial 125 98799 1000 98035 

1.0mL Clear Taper Vial 500 97269 1000 98496 

8x30mm Crimp Top Vials (Use 8mm Closures Below) 

0.5mL Clear, Flat Bottom 200 98819 1000 98823 

0.5mL Amber, Tapered Bottom — — 500 97260 

Sleeve for Tapered Vial (98183) 50 98146 — — 


500µL Amber, Tapered Bottom — — 500 97264 

700µL Amber, Round Bottom 125 98403 500 98411 

Sleeve for 7x32mm Vials 40 98415 — — 


700µL Clear, Tapered Bottom 125 98148 1000 98150 

700µL Amber, Tapered Bottom 200 98894 1000 95171 

800µL Clear, Flat Bottom 200 98043 1000 98045 

800µL Amber, Flat Bottom 200 98896 1000 95170 

6x32mm Crimp Top Vials (not shown) (Use 8mm Closures Below) 

6x32mm Tapered, 0.2mL 100 94470 1000 94472 

6x32mm Round, 0.3mL 100 94450 1000 94463 

Black Support Sleeve for 

20 94455 — — 

HP1090A 

White Support Sleeve for HP1050, 20 94460 — — 

7673A 

Miscellaneous Vials, Clear Glass (not shown) (Use 11mm Seals) 

12x40mm, Use 11mm Seal 100 98273 1000 98570 

10x32, 0.9mL, Use 11mm Seal 500 98226 5x200 98572 

Support Sleeve for 98226 100 98217 — — 

Alcott Micromeritics Vials (not shown) 

8x35mm, Clear Shell Vial with 200 98903 1000 50303400 

Plugs, 1mL 

Shimadzu ® Autosampler Vials and Tubes 

Vial, 12x96mm, 5mL, 10/425 100 98187 1000 98564 

Threads 

Tube, 12x96mm, 5mL, No Cap 100 98375 1000 98379 

Required 

DuPont ® Autosampler Vials (Use 13/425 Screw Cap) 

13x65mm, 4mL, 13/425 Threads 125 98821 8x125 98566 

13x100mm, 8mL, 13/425 Threads 125 98250 8x125 98568 

4mL 

vials 

5mL 

Vial 

8mm Closures 

5mL 

Tube 

4723 4724 

8mL 

Aluminum Seals 

with Septa 

4743 4744 

Polypropylene 

Snap Seal 

8mm Closures 


Aluminum Seals with Septum 

Silver Seal with PTFE/Butyl Rubber 100 94831 1000 95172 

Septum 

Silver Seal with PTFE/Silicone Septum 100 94832 1000 94833 

Silver Seal with PTFE/Silicone/PTFE 100 94251 1000 94253 

Septum 

Silver Seal with Black Fluoroelastomer 100 94834 1000 94835 

Polymer Septum—50mil 

Polypropylene Snap Seal 100 83122 1000 83130 



Silanized Glassware 

4711 

Silanized Vials 


12x32mm Vials 

Clear, Standard Mouth, 4mm i.d., 8/425 Thread 100 951911 

Amber, Standard Mouth, 4mm i.d., 8/425 Thread 100 951941 

Clear, Wide Mouth, 6mm i.d., 10/425 Thread 100 981331 

Amber, Wide Mouth, 6mm i.d., 10/425 Thread 100 981351 

Clear, R.A.M. Robotic, 6mm i.d., 9mm Thread 100 953861 

Amber, R.A.M. Robotic, 6mm i.d., 9mm Thread 100 953881 

8x40mm Shell Vials 

Clear 100 886101 

15x45mm Vials 

Clear, 13/425 Thread 100 981101 

Amber, 13/425 Thread 100 981201 

more info 

See individual product pages for a complete listing of closure 

options. 

tech tip 

Use of silanized glassware can often improve the separations 

of polar compounds such as antibodies, amines, pesticides, 

phenols, and proteins. Pharmaceutical compounds in solution and 

metabolites are especially sensitive and often absorb to the walls 

of an untreated glass container destroying any quantitative value 

the solution might have. 

Our HMDS, high temperature silanization process eliminates 

active sites and prevents sample loss or degradation. 

Silanized Inserts and Specialty Vials 


Limited Volume Inserts for Vials 

100µL for 12x32 Standard Mouth 100 952018 

250µL for 12x32 Wide Mouth 100 983228 

250µL with PE Bottom Spring for Wide Mouth 100 99060 

100µL for 1mL WISP 96 Style Vials, 8x40 100 989008 

300µL for 15x45 WISP Style Vials 100 981188 

Silanized Culture Tubes 

13x100mm Culture Tubes 1000 985911 




Special Silanized Vials 

300µL Crimp Top Microvials, 8mm Seals with 

PTFE/Butyl Septa 

100 944508 

more info 

Convenience kits simplify ordering with one part number to 

remember. Kits provide both vials and caps for a perfect fit 

every time. 

best 

value 

4932 

Silanized Vial Kits 


12x32mm (2mL) Silanized Screw Cap Vial kits 

Clear, Standard Mouth Vials, 8/425 Caps, 

100 72666 

PTFE/Silicone Septa 

Amber, Standard Mouth Vials, 8/425 Caps, 

100 72670 


Clear, Wide Mouth Vials, 10/425 Caps, 

100 981338 


Amber, Wide Mouth Vials, 10/425 Caps, 

100 981358 


Clear, R.A.M. Robotic Vials, 9mm Caps, 

100 953868 


Amber, R.A.M. Robotic Vials, 9mm Caps, 

100 895388 


1mL WISP Style 96 Silanized Vial Kit 

Clear Vials with Clear Snap Plugs 100 982108 

15x45mm (4mL) Silanized Screw Thread Cap Vial Kits 

Clear Vials, 13/425 Caps, PTFE/Silicone Septa 100 72676 

Amber Vials, 13/425 Caps, PTFE/Silicone Septa 100 72680 

vials 

4741 


Custom Silanization Service 

Have we missed a vial or tube 

size that you need? Call to 

inquire about custom silanization 

for any vial or tube size that you 

need for your application, C-8500. 


367


Screw Thread Vials and Storage Bottles 

Screw Cap Vials 

Boston Round Screw Cap Bottles 

4745 

Screw Cap Vials and Bottles for Sample Storage 

Glass 

Color 

Neck 

Finish 

Dia x Ht 

(mm) Qty. Part No. Qty. Part No. 

Volume 

Screw Cap Vials 

2mL Clear 8/425 12x32 100 95191 1000 95192 

2mL Amber 8/425 12x32 100 95194 1000 95195 

2mL Clear 10/425 12x32 100 98133 1000 98433 

2mL Amber 10/425 12x32 100 98135 1000 98435 

4mL Clear 13/425 15x45 100 98110 1000 98207 

4mL Amber 13/425 15x45 100 98120 1000 98484 

8mL Clear 15/425 17x60 144 98862* 200 988621 

8mL Amber 15/425 17x60 144 98864* 200 988641 

14–16mL Clear 18/400 21x70 144 98866* 200 988661 

14–16mL Amber 18/400 21x70 144 98186* — — 

24mL Clear 20/400 23x85 144 98868* 200 988681 

40mL Clear 24/400 28x95 100 98912 1000 98914 

Boston Round Screw Cap Bottles 

30mL–1oz Clear 20/400 31x79 12 9529 — — 

30mL–1oz Amber 20/400 31x79 12 9536 — — 

60mL–2oz Clear 20/400 39x94 12 9531 — — 

60mL–2oz Amber 20/400 39x94 12 9537 — — 

125mL–4oz Clear 22/400 48x112 12 9523 — — 

125mL–4oz Amber 22/400 48x112 12 9538 — — 

250mL–8oz Clear 24/400 60x136 12 9535 — — 

250mL–8oz Amber 24/400 60x136 12 9539 — — 

480mL–16oz Amber 28/400 75x168 12 95391 — — 

960mL–32oz Clear 33/400 94x206 12 95392 — — 

*Includes vials and caps with white styrene butadiene rubber septa. 

4746 

PTFE Septum Vials 

• Usable to 250°C 

• Ideal for alkaline reactions 

• Useful for silylation, acylation, alkylation, and 

hydrolysis reactions 

• Do not use as a pressure vessel 

Sampling Devices 

Volume Qty. Part No. 

PTFE Mini-Vials (Supplied with Caps and Septa)** 

5mL PFA Mini-Vials, 2.54x3.5cm 10 97050 

7mL PFA Mini-Vials, 2x3.4cm 10 97070 

15mL PFA Mini-Vials 10 97150 

30mL PFA Mini-Vials 10 97300 

23mm PTFE/Silicone Septum, Fit 5, 7mL 30 95363 

31mm PTFE/Silicone Septum, Fit 15, 30mL 30 95373 

24mm Mininert ® Valve, Fit 5, 7mL 12 95326 

**Vials are made of PTFE, a perfluoroalkoxy copolymer with properties similar to PTFE 

and FEP. 


Refer to pages 380–381 for racks 

and storage containers. 

vials 

more info 

For Screw Thread Closures see page 369. 

4951 



Screw Thread Closures 

Open-Hole Screw Thread Caps, Unlined 

Open-Hole Screw Thread Caps, Unlined 

Neck Finish Description Qty. Part No. 

Open-Hole Screw Caps, Unlined, Polypropylene 

8/425 Black Open-Hole Cap 100 73044 











Septa for Open-Hole Caps 

4991 

Screw Thread Caps with Molded Septa 

• One-piece “Top Seal” closure saves time 

• 0.010" molded septum allows easy needle 

penetration 

• Polypropylene construction for solvent 

resistance 

Top Seal Closures with Septa—10mil 


Top Seal Closures with Molded Polypropylene Septa—10mil 

8/425 Clear Cap with Polypropylene Septum 100 98060 






Solid Top Screw Caps with Septa 

• Convenient for sample storage 

4989 

4990 

Septa for Open—Hole Caps 


Septa for Open—Hole Screw Thread Caps 

8/425 PTFE/Silicone Septum—5/55mil 100 73048 



















Open-Hole Screw Thread Caps with Septa 

Polypropylene Screw Thread Caps with Septa 


Open-Hole Screw Caps with PTFE/Silicone Septa— 60mil 

8/425 Black Cap with PTFE/Silicone Septum 100 98061 



Open-Hole Screw Caps with PTFE/Silicone/PTFE Septa— 40mil 

8/425 Black Cap with PTFE/Silicone/PTFE Septum 100 98701 



4683 

4761 

Solid Screw Thread Caps and Septa 


Solid Screw Thread Caps with Interseal PTFE/Silicone Bonded Septa 

13/425 White Cap with PTFE Septum 100 99031 






Phenolic Solid Screw Thread Caps with PTFE/F217* Septa 

15/425 Green Cap with PTFE Septum 50 95269 






Polypropylene Solid Screw Thread Caps with PTFE Septa 

8/425 Black Cap with PTFE Septum 100 98063 






Polypropylene Solid Threaded Cap with Polyethylene-Cone Septa 

13/425 Black Cap with Poly-Cone Septum 100 95247 








Polypropylene Solid Screw Thread Caps, Unlined 

8/425 Black Cap 100 98105 

10/425 Black Cap 100 98162 

13/425 Black Cap 100 98614 

*F217—Polyethylene foam material. 

vials 


369


Crimp Top Vials and Storage Bottles 

Crimp Top Containers Provide Positive 

Seal and Serve as Ideal Storage Vessels 

• Borosilicate glass containers 

• Supplied without seals 

Serum bottles are made of borosilicate glass 

that has a higher coefficient of expansion than 

autosampler vials. They have less resistance to 

sudden temperature changes. 

4750 

Crimp Top Vials and Bottles 

Glass 

Color 

Neck 

Finish 

Dia x Ht 


Volume 

Crimp Top Vials and Bottles 

0.3mL Clear 11mm 12x32 100 95240 1000 — 

0.3mL Amber 11mm 12x32 100 95268 1000 — 

0.5mL Clear 8mm 8x30 200 98819 1000 98823 

0.8mL Clear 8mm 7x40 200 98043 1000 98045 

0.8mL Amber 8mm 7x40 200 98896 1000 95170 

1mL Clear 8mm 8x40 125 98799 1000 98035 

1.5mL Clear 11mm 12x32 100 72009 1000 — 

2mL Clear 11mm 12x32 100 66002 1000 66003 

2mL Amber 11mm 12x32 100 95221 1000 95222 

2mL Clear 11mm 12x40 100 98273 1000 98570 

4mL Clear 13mm 15x45 100 98040 1000 98240 

4mL Amber 13mm 15x45 100 98097 1000 98297 

6mL Clear 20mm 22x38.5 100 99056 1000 99058 

9mL Clear 20mm 18x50 125 98098 1000 98548 

10mL Clear 20mm 22.5x46 100 6655 1000 66551 

12mL Clear 20mm 18x65 125 98104 1000 98877 

20mL Clear 20mm 22.5x75.5 100 6636 1000 66361 

27mL Clear 20mm 30x60 100 98093 1000 98546 

Crimp Top Serum Bottles 

4765 

Crimp Top Bottles and Stoppers 

Glass 

Color 

Neck 

Finish 

Dia x Ht 


Volume 

Crimp Top Vials and Bottles, Wheaton 400 

2mL Clear 13mm 15x40 288 66021 — — 

5mL Clear 20mm 23x47 288 66041 — — 

10mL Clear 20mm 25x54 288 66036 — — 

30mL Clear 20mm 37x63 288 66038 — — 

50mL Clear 20mm 43x73 288 66040 — — 

100mL Clear 20mm 52x95 144 66042 — — 

Stoppers for Serum Bottles 

11mm Natural Rubber Stoppers 100 6626 1000 66261 


13mm Butyl Rubber Stoppers 72 95245 1000 95252 


20mm Butyl Rubber Stoppers 72 95305 720 98811 

vials 

Stoppers 

more info 

For Aluminum Seals and Septa see page 371. 

more info 

For more information on Crimpers and Decappers, 

see page 383. 

4766 

Crimpers and Decappers 

Volume Description Qty. Part No. 

Adjustable Hand Crimpers for Crimp Top Vials 

8mm Hand Crimper* ea 666008 

11mm Hand Crimper ea 60041 



Hand Decappers for Crimp Top Vials 

8mm Hand Decapper ea 98709 




Plier Decappers for Crimp Top Vials 

8mm Plier Decapper ea 6662 




*Not adjustable. 



Aluminum Seals and Septa 

Aluminum Seals with Septa 

• One-piece design saves time by eliminating the process 

of inserting septa into the closures 

• For maximum sample protection, the seal should be 

replaced before storing the sample 

• Available in a wide assortment of colors 

Aluminum Seals, Unlined 

• Also a vailable in a wide assortment of colors. 

Call for additional colors. 

4999 

5003 

Aluminum Seals with Septa 

Description Size Qty. Part No. 

Seal with PTFE Septum—10mil 

Silver Seal 11mm 100 98013 

1000 98113 

13mm 100 98101 

1000 98103 

20mm* 100 99103 

1000 99105 

Seal with PTFE/Butyl Septum 


1000 95172 

11mm 100 73070 

1000 73071 

13mm 100 98776 

1000 98206 

20mm 100 98801 

500 98552 

Red Seal 11mm 100 98051 

Blue Seal 11mm 100 98053 

Green Seal 11mm 100 98057 

Gold Seal 11mm 100 98059 

Seal with PTFE/Silicone —10/50mil 


1000 94833 

11mm 100 98740 

1000 94867 

13mm 100 98283 

1000 98289 

20mm 100 95756 

1000 95758 

Seal with PTFE/Silicone/PTFE 


1000 94253 

11mm 100 94855 

1000 94865 

13mm 100 98596 

1000 98598 

Seal with PP Septum—10mil 


Seal with Pharma-Fix Septum 


20mm 100 6689 

Seal with Black Fluoroelastomer Polymer Septum 


1000 94835 

11mm 100 94852 

1000 94862 

*Not suitable for headspace seals. 

4754 

Aluminum Seals, Unlined 


Standard Seals 

Silver—Open Center 11mm 100 98746 

1000 66405 

Silver—Center Tear-Off 13mm 100 98778 

1000 66410 

Silver—Center Tear-Off 20mm 144 66440 

1440 98803 

Tear-Away 

Silver 13mm 100 98789 

1000 66416 

20mm 144 66501 

1440 98805 

Magnetic Tinplate 

Silver 20mm 100 98532 

Septa for Aluminum Seals 

• Refer to the septum compatibility 

guide on page 349 to choose 

the septum best suited for your 

application 

Septa for Aluminum Seals 


White PTFE Septum—10mil 11mm 100 98007 

13mm 100 98177 

PTFE/Silicone Septum— 60mil 11mm 100 98009 

1000 98019 

13mm 100 98791 

1000 95253 

PTFE/Silicone Septum—100mil 13mm 100 98793 

20mm 72 95313 

PTFE/Silicone/PTFE Septum— 40mil 11mm 100 98010 

1000 98020 

13mm 100 98180 

1000 98182 

PTFE/Butyl Rubber Septum— 40mil 11mm 100 98398 

1000 98400 

13mm 100 98173 

1000 98175 

Red Butyl Septum 20mm 100 98695 

Red Silicone Septum 20mm 72 98536 

1000 99108 

PTFE/Gray Butyl Septum 20mm 72 98540 

Aluminum/Silicone Septum— 60mil 20mm 72 98665 

more info 

For a complete listing of 20mm Aluminum Seals and Septa 

see page 365. 

4756 

vials 


371


Shell Vials and Kits 

Shell Vials with Polyethylene Conical 

Starburst † Snap Plug Style Closures 

• Thicker glass walls for safer handling 

• Special safety plug eliminates sore fingers when pressing 

plugs into shell vials 

15x45mm 


12x32mm 

Conical Snap Plug 

with pull tab and 

starburst 

4938 

8x40mm 

Conical Snap 

Plug with 

starburst 

Shell Vial Kits and Replacement Caps 

100/pk 

Part No. 

1000/pk 

Part No. 

Description 

8x40mm (1mL) Kit Includes: Shell Vials, Clear Conical Snap Plugs 

Clear Vials, 200/pk 88631** 98834 

Amber Vials, 200/pk 88612** 98838 

Polypropylene Vials, 200/pk 88626** 98224 

Limited Volume Shell Vials (use Conical Plugs, not supplied with vials) 

700µL Polypropylene, 8x40mm 98990 98992 

8mm Replacement Polyethylene Conical Starburst Snap Plugs † 

Clear Plug 98994 98996 

Red Plug 99003 99013 

Blue Plug 99005 99015 


Clear Vials 98840 98843 

Amber Vials 98845 98847 

Polypropylene Vials 98849 98851 

Polypropylene Vials for Dionex 91450 91455 


Clear Plug 98980 98989 

Red Plug 99033 99053 

Blue Plug 99035 99055 


Clear Vials 98853 98857 

Amber Vials 98859 98863 

Polypropylene Vials 98867 98869 

Limited Volume Shell Vials (use Conical Plugs, not supplied with vials) 

15x45mm Polypropylene, 3mL 98972 98974 


Clear Plug 98976 98978 

Red Plug 99023 99043 

Blue Plug 99025 99045 

Green Plug 99027 99047 


Safety Plug for Inserting Plugs into Any Size Shell Vial 

Multi-Plug Safety Sleeve, 5/pk 96431** — 

† 

With Starburst for easier needle penetration. 

**Correct quantity specified in description. 

4939 

vials 

8x40mm 

15x45mm 

TITESEAL ® Disposable Glass Shell Vials 

and Polyethylene Plug Style Closures 

• Maximum temperature use of 80°C for stopper 

TITESEAL ® Disposable Shell Vial Kits 

200/pk 

Part No. 

1000/pk 

Part No. 

Description 

Unassembled Disposable Vial Kits 

8x30mm (0.75mL), Clear Vials and Plugs 98824 73101 

8x43mm (1mL), Clear Vials and Plugs 98578 73146 



Replacement Plugs for TITESEAL ® Disposable Vials 

Plugs for 8mm Vials, 500/pk 73108** — 



**Correct quantity specified in description. 

4940 

15x45mm 12x31mm 8x43mm 8x30mm 



Polypropylene Vials for Ion Chromatography 

Polypropylene vials eliminate interferences associated with glass 

surfaces. 

tech tip 

Polypropylene containers 

Use of polypropylene containers is important to avoid the 

possibilities of interference from metallic ions that can be leached 

from glass containers under certain sampling conditions. 


Looking to further eliminate interferences in your samples 

for IC? 

See our IC SPE devices on pages 304 and 310. 

6521 

Polypropylene Vials and Caps 

100/pk 

Part No. 

1000/pk 

Part No. 

Description 

12x32mm Standard Mouth Screw Thread Vials, 4mm i.d. 

600µL Polypropylene Vial, 8/425 Thread, 500/pk — 89073* 

100µL Polypropylene Vial with 8/425 Thread 12962 12963 


12x32mm Wide Mouth Screw Thread Vials, 6mm i.d. 

Kit—Includes Polypropylene Vial (98099), 

88538 — 

Red 10/425 Cap and 10mil PTFE Septum 



10/425 Black Cap with PTFE/Butyl Septum 97274 97216 


12x32mm Robotic LV Vials, 6mm i.d. 

1.5mL Clear Polypropylene Vial 99098 99140 

1.5mL Amber Polypropylene Vial 99109 99142 

100µL Polypropylene R.A.M. Vial 90273 90271 

9mm Blue R.A.M. Cap, PTFE/Silicone Septum 95360 95359 

12x32mm Crimp Style Vials, use 11mm Seal 

750µL Polypropylene Vial 98050 98215 

600µL Polypropylene Vial, 500/pk — 88363* 




11mm Aluminum Seal with PTFE/Butyl Septum 73070 73071 

11mm Aluminum Seal with PTFE/Silicone Septum 98740 94867 

11mm Poly Crimp Seal with PTFE/Butyl Septum 95232 95332 

11mm Poly Crimp Seal with PTFE/Silicone Septum 95234 95334 

8x30 and 8x40mm Shell Vials 

8x30mm Shell Vial, 900µL 99184 99192 

8x30mm Limited Volume Vial, 500µL 99178 99182 

8x40mm Polypropylene Shell Vial, 1mL 88625 98827 

8mm Polyethylene Starburst Snap Plug 88613 98865 

700µL LVI Polypropylene vial 98990 98992 

8mm Polyethylene Conical Plug 98994 98996 

15x45mm Vials 

2.5mL Polypropylene Vial, 13/425 Thread 98091 98491 

1.2mL Polypropylene Vial, 13/425 Thread 70149 70198 


3mL Polypropylene LVI Shell Vial 98972 98974 

13mm Clear Conical Plug 98976 98978 

2.5mL Crimp Style 98054 98254 

13mm Snap TOP Cap, PTFE/Silicone Septum 98044 98244 

*Actual quantity listed in description. 

For Polypropylene Limited Volume Inserts see: 

more info 

For 4mm i.d. Standard Mouth Vials, see page 361. 

more info 

For 6mm i.d. Wide Mouth Vials, see page 361. 

vials 

more info 

For 8x40mm Shell Vials, see page 354. 

4748 

more info 

For 15x45mm Vials, see page 362. 

Limited volume inserts provide greater access to small sample 

volumes. 


373


Finneran Versa-Vials 

• Secure seal for sample integrity 

• Vase-like neck allows pick up with 

autosampler’s robotic arm 

• Selection of closures improves sample 

compatibility 

Easy use of pipet 

tips with 9.5mm i.d. 

opening. 

A new generation of 12x32mm shell vials to upgrade and automate your shell vial sampling. 

6460 

Soft press-in plugs 

eliminate breakage 

when capping. 

6461 

Versa-Vials and Plug Closures 

100/pk 

Part No. 

1000/pk 

Part No. 

Description 

Versa-Vials, 12x32mm, 1.8mm 

Clear Glass 99063 99163 

Amber Glass 99064 99164 

Clear Glass with Marking Spot 99066 99166 

Amber Glass with Marking Spot 99067 99167 

Limited Volume Inserts with Flanged Top 

250µL Glass, LVI 99095 99195 

250µL Polypropylene LVI 99089 99191 

350µL Polypropylene Flat Bottom 99183 99189 

Plug Closures 

PTFE/Silicone Plug with Slit* 99080 99082 

PTFE/Silicone Plug 99085 99087 

PTFE/Gray Chlorobutyl Plug 99090 99092 

Polyethylene Plug with Conical Starburst 99094 99096 

*Prevents bent needles. 

6461 

Tubes and Vials for Robotics 

vials 

Tubes and Vials for Robotics 


Culture Tubes, Borosilicate Glass 

10x75mm Tubes, 5mL 250 98829 1000 98590 

12x75mm Tubes, 6mL 250 98585 1000 98587 

13x100mm Tubes, 10mL 250 98589 1000 98591 

16x100mm Tubes, 15mL 250 98593 1000 98595 

20x150mm Tubes, 23mL 125 98597 500 98599 

25x150mm Tubes, 60mL 125 98601 500 98603 

Glass Autosampler Vials, Caps Sold Separately 

12x32mm Clear, Crimp Top, 11mm 100 66002 1000 66003 

12x32mm Amber, Crimp Top, 11mm 100 95221 1000 95222 

15x45mm Clear, 13/425 Screw Thread 100 98110 1000 98207 

15x45mm Amber, 13/425 Screw Thread 100 98120 1000 98484 

Scintillation Vials (Supplied with White Urea Caps with Cork Foil Liners) 

20mL Glass Scintillation Vials, 28x57 100 98613 500 98615 

20mL Polyethylene Scintillation Vials 100 98617 500 98619 

0522 

more info 

See also R.A.M. Vial page 355. 



Mini-Vials, Maxi-Vials, and Accessories 

Mini-Vials and Maxi-Vials 

• Heavy wall, clear 

borosilicate glass 

• Tapered cone for 

maximum recovery 

• Mini-vial starter kit 

contains 2 each of 

the 0.3, 1.0, 3.0 and 

5.0mL clear vials 

Mini-Vials and Accessories 


Clear Mini-Vials and Maxi-Vials (Supplied with Screw Caps) 

0.1mL Mini-Vials, 13/425 12 95001 

0.3mL Mini-Vials, 13/425 12 95003 

1.0mL Mini-Vials, 13/425 12 95010 

2.0mL Mini-Vials, 20/400 12 95020 

3.0mL Mini-Vials, 20/400 12 95030 

5.0mL Mini-Vials, 20/400 12 95050 

10.0mL Maxi-Vials, 20/400 12 95100 

Mini-Vial Starter Kit 

2 ea of 0.3, 1.0, 3.0 & 5.0mL Clear Vials ea 9511 

Caps 

13/425 Replacement Phenolic Caps 48 95090 

20/400 Replacement Phenolic Caps 48 95053 

Crimp Top Micro-Reaction Vials 

• Reusable, crimp-top mini-vial 

• Use 20mm aluminum seals 

• Cone-shaped interior for sample retrieval 

4757 


• PTFE is only sample contact 

• Replace septum without exposing 

sample 

• Maximum pressure = 120psig 

• Use 20–26 gauge needles 

• Do not autoclave 

Mininert ® Valves and Accessories 



13mm Mininert ® Valve for 13/425 Neck ea 9544 

12 95246 

15mm Mininert ® Valve for 15/425 Neck 12 95266 


20mm Mininert ® Valve for 20/400 Neck ea 9546 

12 95306 


Other Mininert ® Valves 

20mm Crimp Top Mininert ® Valve 12 66806 

Standard Taper Valve, 14/20 ea 621102 

Standard Taper Valve, 19/22 ea 621103 

1/4-28 Male to Mininert ® ea 631201 

1/4-28 Female to Mininert ® ea 631203 

1/4-28 Male to 1/4-28 Male ea 631205 

1/4-28 Female to 1/4-28 Female ea 631206 

1/8" Male NPT to Mininert ® ea 631202 

1/8" Female NPT to Mininert ® ea 631204 

Mininert ® Accessories 

Mininert ® Septum Inserter ea 644850 

Replacement Mininert ® Septa 12 9548 

50 644350 

4760 

Crimp Top Micro-Reaction Vials 


0.3mL Vials, 20mm Septa and Seals 5 9494 



20mm Standard Aluminum Seals 144 66440 

20mm Tear-Away Seals 144 66501 

Liners for 20mm Micro-Reaction Vials 12 9493 

Microflasks 

• Ideal for extraction of trihalomethanes 

(EPA Method 501.2) 

• Use screw-caps or 20mm 

Mininert ® valves 

• Do not autoclave 

4758 

4759 

Magnetic Stirring Bars 

• PTFE-coated, octagonals with 

a molded pivot ring 

Magnetic Stirring Bars for Vials 


Spin Vanes, for 0.3–1mL Mini-Vials 6 903061 

Spin Vanes, for 2–10mL Mini-Vials 6 903063 

Stirring Bars, 1.5x8mm 6 9670 

Stirring Bars, 3x12.7mm 6 9672 

Stirring Bars, 8x16mm 6 9674 

Stirring Bars, 8x25.4mm 6 9676 



PTFE Coated Stirring Retriever*, 9.5x300mm ea 9682 

*Polyethylene. 

4960 

vials 

Microflasks 


10mL Microflask 12 9551 

25mL Microflask 12 9553 

Open-Hole Screw Caps, 20/400 72 95301 

PTFE/Silicone Septa for Screw Caps 72 95302 

PTFE/Silicone Septa, 10/90 for Caps 72 95303 


375


EPA Sample Containers 

Variety of storage bottle sizes and styles in glass or 

polyethylene. Available unwashed or cleaned to meet 

requirements for VOA analyses 


Working on an EPA method? 

Refer to pages 232–234 for our 

Guide to Capillary Columns for 

Environmental Analysis. 

Septa Bottles 

4944 

Wide Mouth Glass Jars 

5318 

Polyethylene (HDPE) Cylinders 

4945 

EPA Sample Containers 

Volume, Description, 

Neck Finish (Cap Size) 

Dimensions 

Ht x Dia Qty. 

Unwashed 

Part No. 

Washed 

Part No. 

Septa Bottles — Supplied with Open Top Poly Caps with 


8oz (250mL), Amber Septa 5-11/16" x 2-3/8" 12 89159 89158 

Bottle, 24/414 

4oz (125mL), Clear Tall 4" x 1-7/8" 24 89099 89079 

Septa Jar, 48/400 

Wide Mouth Glass Jars — Supplied with Poly Caps with PTFE Septa 

4oz (125mL), Clear Tall 4" x 1-7/8" 24 89198 89169 

Glass Jar, 48/400 

4oz (125mL), Clear Short 2-1/8" x 2-3/8" 24 89309 89308 


4oz (125mL), Amber Glass 3-13/16" x 2" 24 89332 89320 

Jar, 38/400 



8oz (250mL), Amber Glass 4-3/4" x 2-1/2" 24 89359 89351 

Jar, 45/400 



16oz (500mL), Amber 5-7/8" x 3-1/8" 12 89360 89353 


32oz (1.0L), Clear Tall 6-1/2" x 3-5/8" 12 89367 89354 


32oz (.95L), Amber Glass 6-1/2" x 3-5/8" 12 89368 89355 

Jar, 53/400 

High Density Polyethylene (HDPE) Cylinders —With Poly Caps and 

Polyfoam Septa 

8oz (250mL), HDPE Round 6-1/8" x 2" 24 89372 89369 

Cylinder, 24/410 

16oz (500mL), HDPE 7-3/8" x 2-1/2" 24 89373 89370 

Round Cylinder, 28/410 

32oz (1.0L), HDPE Round 

Cylinder, 28/410 

9-3/8" x 3" 12 89374 89371 





6534 

vials 



EPA Vials for Volatile Organic Analyses 

• Available washed* or unwashed in 20, 40, and 60mL vial 

sizes and in 12x32 and 15x45mm autosampler vial sizes 

• Washed* EPA vials are supplied with a certificate of 

analysis—equivalent to I-CHEM Series 3 

• Washed vials are recommended for the analysis of 

volatile organics 

• All vials are borosilicate glass 

• Three septum choices for maximum versatility—0.125" 

for EPA/VOA Samples, 0.125" TOP Hat Septum Locks 

Securely Into Cap, 0.06" for autosampler use (purchase 

separately) 

• All are supplied as kits with vial and cap specified 

*Washed to Protocol B specifications. 

Cleaning Protocol B 

1. Wash bottles, septa, and caps in laboratory grade, biodegradable, 

non-phosphate detergent. 

2. Rinse three times with tap water. 

3. Rinse three times with ASTM Type one deionized water. 

4. Oven dry. 

5. Assemble in organic-free environment. 

tech tip 

Advantages of TOP Hat Closure 

• Molded TOP* Hat PTFE/Silicone septum locks into its unique 

cap opening 

• Prevents septa falling out of caps 

• Available for washed or unwashed vials in all sizes 

Cross-Section Cap and Septum 

125 mil Septum 

*Time Oriented Products. 

TOP Hat Septum locks securely 

into the black cap. 

6296 

EPA/VOA Vial Kits 

Description 

Qty. 

Unwashed 

Part No. Qty. 

Washed 

Part No. 

20mL Vials 

Clear Vials, White Caps, 

72 89515 72 89517 


Amber Vials, White Caps, 

72 89516 72 89518 


Clear Vials, Solid White Cap, PTFE — — 72 89325 

Septum 

Clear Vials, Black Caps, 0.125" TOP 72 98641 72 98643 

Hat PTFE/Silicone Septa* 

40mL Vials 


72 89525 72 89527 


Amber Vials, White Caps, 

72 89526 72 89528 


Clear Vials, Solid White Cap PTFE — — 72 89326 

Septum 

Amber Vials, Solid White Cap, 

— — 72 89199 

PTFE Septum 

Clear Vials, Black Caps, TOP Hat 72 98649 72 98651 

PTFE/Silicone Septa* 

60mL Vials 


72 89535 72 89537 


Clear Vials, Solid White Caps with — — 24 89327 

PTFE Septa 

Clear Vials, Black Caps, TOP Hat 24 98657 24 98659 

PTFE/Silicone Septa*, 24/pk 

12x32mm (2mL) Vials with Cap and Septum 

Clear Screw thread (8/425) Vial, Cap 100 98625 100 98627 

with PTFE/Silicone/TOP Hat Septum 

15x45mm (2mL) Vials with Cap and Septum 

Clear Screw thread (13/425) Vial, Cap 100 98633 100 98635 

with PTFE/Silicone/TOP Hat Septum 

24/414 Replacement Caps with Septa for EPA Vials, Unwashed 

White Caps with 0.060" 

100 89519 — — 


White Caps with 0.060" 

1000 89520 — — 


Black Caps, TOP Hat 

100 98085 — — 


Black Caps, TOP Hat 

1000 98079 — — 


White Cap Only 100 89226 — — 

0.125" Septum for EPA Vials 100 89277 — — 

*Patented TOP Hat Closure with 125mil septum meets EPA Requirements. 

vials 


377


I-CHEM Vials 

• 20, 40, and 60mL I-CHEM vials 

for EPA volatile organic analyses 

• All vials are borosilicate glass 

• Choice of closure/liner for 

maximum versatility—0.06 for 

autosampler use, 0.125 for 

EPA/VOA samples 

I-CHEM Sample 

Containers 

Septa Bottles and Jars 

• For analysis of volatile organics 

Wide Mouth Glass Jars 

• For semi-volatile, PCBs/pesticide, metals, 

and cyanide analysis 

4944 

4943 

I-Chem EPA/VOA Vials—Series 2** 


Processed* I-CHEM Vials, Assembled with Caps and Septa 

20mL Vials, Processed 

Clear Vials, 24/414 Open-Hole Caps, 0.125" PTFE/Silicone Septum 72 98702 

Clear Vials, 24/414 Solid Caps, PTFE Septum 72 98704 



Amber Vials, 24/414 Open-Hole Caps, 0.125" PTFE/Silicone Septum 72 98708 




Amber Vials, 24/414 Solid Caps, PTFE Septum 72 98716 




Unwashed I-CHEM Vials, Assembled with Caps and Septa 

20mL Vials, Unwashed 

Clear Vials, Open-Hole Caps, 0.125" PTFE/Silicone Septum 72 98682 




Amber Vials, Open-Hole Caps, 0.125" PTFE/Silicone Septum 72 98688 




Amber Vials, 24/414 Solid Caps, PTFE Septum 72 98696 




*Processed vials are recommended for use in analysis of volatile organics. 

**Certificate of analysis is available by lot number. Request after purchase. 

Replacement Caps and Septa for I-CHEM Vials 


Caps Only, 24/414, Open Top without Septum 24 92414 

Septa Only, 0.125" PTFE/Silicone 24 92416 

vials 

Polyethylene (HDPE) Round Cylinders 

• For metals and cyanide analysis 

5318 

4945 

I-CHEM Sample Containers 

Neck Finish 

(Cap Size) 

Dimensions 

Ht x Dia Qty. Part No. 

Volume 

Description 

Septa Bottles — Supplied with Open Top Poly Caps with PTFE/Silicone Septa 

8oz (250mL) Amber Septa Bottle 24/414 5-11/16" x 2-3/8" 12 98720 

4oz (125mL) Clear Tall Septa Jar 48/400 4" x 1-7/8" 12 98722 

Wide Mouth Glass Jars — Supplied with Poly Caps with PTFE Septa 

4oz (125mL) Clear Tall Glass Jar 48/400 4" x 1-7/8" 12 98718 

4oz (125mL) Clear Short Glass Jar 58/400 2-1/8" x 2-3/8" 24 98732 

4oz (125mL) Amber Glass Jar 38/400 3-13/16" x 2" 12 98734 

8oz (250mL) Clear Tall Glass Jar 58/400 4-13/16" x 2-1/4" 12 98698 


8oz (250mL) Amber Glass Jar 45/400 4-3/4" x 2-1/2" 12 98700 


16oz (500mL) Amber Glass Jar 53/400 5-7/8" x 3-1/8" 12 98738 

32oz (1.0L) Clear Tall Glass Jar 89/400 6-1/2" x 3-5/8" 12 98399 

32oz (.95L) Amber Glass Jar 53/400 6-1/2" x 3-5/8" 12 98730 

High Density Polyethylene (HDPE) Cylinders —with Poly Caps and Polyfoam Septa 

8oz (250mL) HDPE Round Cylinder 24/410 6-1/8" x 2" 24 98724 

16oz (500mL) HDPE Round Cylinder 28/410 7-3/8" x 2-1/2" 24 98726 

32oz (1.0L) HDPE Round Cylinder 28/410 9-3/8" x 3" 12 98728 



96-Well Products 

Glass Inserts for 96-Well Plates 

for Waters ® Alliance ® HT System* Ritter Style Plate 

• Push-point tip design allows sample access to 10µL 

• Snug fit allows use with shakers and vortexers 

• Use molded covers or 8mm snap plugs for inserts 

• Ideal solution for reactive chemistry applications 

96-Deep Well 

Microplates and 

Cover Mats 

• Standard 8x12 format 

• Borosilicate glass plates 

• Polypropylene square or round wells 

3488 4348 

Microplates and Accessories 

Description, Well Capacity, Well Volume Qty. Part No. 

All-Glass 96-Well, Class A Borosilicate Plates 

Glass, Flat Bottom, 500µL 1 91504 

96-Well Square Deep Microplates and Caps 

Polypropylene, Square Deep Well, 2mL 100 80910 

Glass inserts eliminate chemical resistance problems associated with 

polypropylene 96-well plates for chromatography analysis. 

Components for 96-Well Ritter Style System 


Borosilicate Glass Inserts for Square Well Plates 

0.7mL Glass Tapered Insert 100 91501 

1.0mL Glass Tapered Insert, Flat Tip 100 91508 

Covers for 1mL Inserts in Plate, Molded Round Well 

PTFE/Blue Silicone 5 91496 

Convenience Kits: Plate with Inserts Inserted 

Plate with 0.7mL Insert 1 91497 

Plate with 1.0mL Insert 1 91505 

Plate with 1mL Flat Tip Insert 1 91509 

*Ritter style plate designed for use in Waters ® Alliance ® HT (2790/2795) system. Inserts 

also work in equivalent deep well 2mL plates. 

96-Well µLPLATES and Inserts 

• In Polypropylene or PTFE 

• Piercible non-coring covers 

• Choice of shallow, medium, or deep wells 

• Chimney well design prevents sample crossover 

Components for µLPlate System 


Microliter 96-Well Plates 

Deep-Well Plates, Polypropylene 

2.0mL, Square Well, V-Bottom Plates 1 70098 

Shallow and Medium-Well Plates, Polypropylene 

500µL, Medium, Round Well, Round Bottom Plates 1 70089 

Shallow-Well Plates, PTFE 

450µL, Round Well, Round Bottom Plates 1 70208 

Borosilicate Glass Inserts for µLPLATES 

For Medium Well Plates* 

350µL Inserts for 12x32mm Vials 100 70048 

350µL Silanized Inserts for 12x32mm Vials 100 70063 

*Use 350µL insert to transfer directly from 96-well plate to 12x32mm robotic 

autosampler vials. 

6778 

Bio-Tech Sealing Systems 

Molded Liners for Round or Square 96-Well Plates 

• Superior resealability, resists coring 

and tearing 

• Tight seal prevents cross 

contamination and 

sample evaporation 

• Autoclavable and excellent 

chemical compatibility 

• Now available in clear pre-slit 

design 

Molded Liners for 96-Well Plates 


Gray PTFE-Lined Silicone 

Round well 5 91458 

Square well 5 91464 

Clear PTFE-Lined Silicone 

Round well 5 8619141 

Round well, Pre-slit 5 8619145 

Square well 5 8619142 

Square well, Pre-slit 5 8619146 

Sealing Films for Round or 

Square Well Plates 

• Superior quality films with uniform, 

consistently applied adhesive 

• Adheres to polypropylene, polystyrene, 

or polycarbonate plates 

• Choices offer temperature and 

chemical resistance 

• Fits all 96-well, 384-well, and 

1536-well plates 

SealPlate 

Blue PTFE 

new 

Sealing Films for 96-Well Plates 

Description Qty Part No. 

Blue PTFE Film, Adhesive-Free Over 96-Well Area 

5mil Thickness 100 91490 

SealPlate for ELISA, EIA, and Similar Assays 

2mil Polyester Film, Non-pierce 100 91487 

Aluminum Foil Film for Light Sensitive Samples 

Pierceable, for Robotics 100 91488 

7441 

6681 

6683 

vials 


379


Racks and Storage Containers 

Polypropylene Vial Storage Racks 

• Choice of sizes to fit 

8 to 28mm o.d. vials 


• Stackable 

4951 

Polypropylene Vial Storage Racks 

Fits Vial 

Diameter 

Holds 

(#Vials) Description Qty. Part No. 

8mm 96 185mm x 127mm x 33mm ea 94838 

8mm 96 185mm x 127mm x 33mm 5 94839 

12mm 50 194mm x 100mm x 23mm ea 94874 

12mm 50 194mm x 100mm x 23mm 5 94875 

15mm 48 270mm x 97mm x 30mm ea 94877 

15mm 48 270mm x 97mm x 30mm 5 94878 

15mm 90 327mm x 172mm x 30mm ea 94879 

15mm 90 327mm x 172mm x 30mm 5 94880 

28mm 50 325mm x 171mm x 30mm/EPA ea 94881 

28mm 50 325mm x 171mm x 30mm/EPA 5 94882 

Polypropylene Rack with Handle 

• Stackable—empty or full 


3432 

Ampul-store 

• Eliminates need to relabel or transfer 

open 2mL ampule contents 

• Minimizes sample odors in the lab 

To use: Break off the ampule top. Place 

the ampule in the Ampul-store and attach 

cap. Inject through the open top screw 

cap/stopper to remove sample. 

ampul-store 


2mL Silanized Amber Ampules 36 72690 

Ampul-store for 2mL Ampules 5 98932 

The Ampule Cracker 

Slip the Ampule Cracker over the top at the 

prescored position, snap it, and discard 

both the ampule top and the safety collar to 

prevent contamination. 

Ampule Cracker 


Ampule Cracker for 1–4mL Ampules 100 01111 

4957 

4958 

Polypropylene with Handle 

Fits Vial 

Diameter 

Holds 


10–33 72 9-3/8" x 4-3/8" x 3-1/2" ea 2035 

13–16 40 10" x 4-3/8" x 3-1/2" ea 2037 

16–20 40 10" x 4-3/8" x 3-1/2" ea 2039 

21–25 40 10-5/8" x 5" x 5" ea 2041 

26–30 24 11-3/4" x 5" x 3-3/4" ea 2043 

Stretch-it—PTFE Sealing Tape 

• Provides a chemically resistant 

cover for reactions in progress 

• Eliminates deteriorated foil and 

paraffin film covers 

• 108ft per roll 

3426 

Metal Storage Cabinet 

• 9-drawer metal cabinet provides 

clean organized vial storage 

• Dimensions: 11" H x 17" W x 11" D 


Stretch-it-PTFE Sealing Tape 


1/2" Wide ea 3241 

3/4" Wide ea 3243 

1" Wide ea 3245 

3" Wide ea 3247 

4" Wide ea 3248 

5" Wide ea 3249 

4952 

vials 

metal storage cabinet 


9-Drawer Metal Storage Cabinet ea 98934 

Plastic Storage Boxes 

• Convenient accessible storage of 

small parts 

• Available with 1, 9, or 18 

compartments 

5772 

Plastic Storage Boxes 


1 Compartment, 9" x 5" x 2" ea 98928 

9 Compartment, 8 of (1-3/8" x 1-5/8") 

ea 6264 

1 of (1-1/8" x 3-1/4") 

18 Compartment, 12 of (2" x 2") 6 of (2" x 4-3/16") ea 6266 



Racks and Storage Containers 

The Polypropylene Snap Rack 

• Stores vials and caps together 

• Stackable 

Polypropylene Gridded Boxes in Neon Colors 

• Hold either 50 or 100 

12x32mm vials 

• Available in blue, green, 

orange, pink, yellow, and 

clear for colorful storage 

and identification 

Polypropylene Snap Rack 


8mm Snap Rack Tray, Holds 96 Vials ea 95076 

12mm Snap Rack Tray, Holds 50 Vials ea 95086 

VialTainer Storage Box 

• Clear polystyrene box 

• Designed for use with 

autosampler vials 

4955 


Raised Edges Provide Non-Slip, Stackable Storage with Writing Panel on 

Lid Front for i.d. 

Alpha-Numeric Code on Grids for Easy Sample i.d. 

Hinged Lid with Secure Snap Clasp 

Rated to -90°C, Ideal for Freezer or Room Storage 

Dimensions: 100 Grid—2.1" H x 6.0" W x 5.5" L 

(53mm H x 152mm W x 140mm L) 

50 Grid—2.1" H x 3" W x 5.5" L 

(53mm H x 76mm W x 140mm L) 

3697 

VialTainer Storage Box 

Fits Vial 

Diameter 

Freezer Racks 

Holds 


12mm 72 Small, 189x94x62mm 4 2049 

15mm 40 Large, 189x94x62mm 4 2051 

• Hold 80 microcentrifuge tubes 


• Polypropylene racks 

Freezer Racks 


Racks, 1 of ea: Red, Blue, Orange, Green, Yellow 5 7363 

3428 

3421 

Neon Storage Boxes 

Fits Vial 

Diameter 

Holds 


To Hold 100 Vials 

12mm 100 Clear 5 95129 

12mm 100 Blue Neon 5 95131 

12mm 100 Green Neon 5 95132 

12mm 100 Orange Neon 5 95133 

12mm 100 Pink Neon 5 95134 

12mm 100 Yellow Neon 5 95135 

12mm 100 Assorted, 1 of ea/5 Colors 5 95005 

To Hold 50 Vials 

12mm 50 Clear 5 95029 

12mm 50 Blue Neon 5 95106 

12mm 50 Green Neon 5 95027 

12mm 50 Orange Neon 5 95028 

12mm 50 Pink Neon 5 95089 

12mm 50 Yellow Neon 5 95108 

12mm 50 Assorted, 1 of ea/5 Colors 5 95008 

Floating Racks 

• Polyfoam racks hold multiple 

tube sizes 

• Ideal for water baths 

Multi-Use Vial Rack System 

• Increased spacing around vials for easy sample access 

• PETG trays snap easily into 

the polypropylene frame 

• Holder and trays sold 

separately 

vials 

Floating Racks 


Small, Hold 0.5 or 0.25mL Tubes 3 7320 

Medium, Hold 0.5 and 1.5mL Tubes 3 7322 

Large, Hold 15 and 50mL Tubes 3 7324 

Assorted, 1 each of Small, Medium, and Large 3 7318 

3413 

Multi-Use Vial Rack System 

Fits Vial 

Diameter 

Holds 


— — Universal Tray Holders 5 98600 

5mm 25 Insert Trays, 98x105mm 5 98671 





4954 


381


Sampling Handling Accessories 

Block Heaters 

• Use to control reaction 

temperatures 

• Use solid block as hot plate or 

drill to fit vials 

The block heater has separate high 

and low thermostats and has an 

on-off switch and pilot light. The low setting is for ambient to 

60°C, while the high setting covers the 50 to 130°C range. 

The heater measures 3" H x 5" W x 8" L (7.6cm H x 

12.7cm W x 20.3cm L). 

All of the blocks listed are 2" H x 2-15/16" W x 3-3/4" L 

(5.1cm H x 7.5cm W x 9.5cm L) and are interchangeable. 

Wells are 1.75" (44cm) deep with rounded bottoms. 

Block Heaters 


Temp-Block*, 110V ea 9707 

Temp-Block*, 220V ea 97071 

Heating Block, 20x13.5mm Holes ea 9708 




Heating Block, Solid ea 9711S 

Thermometer, -20 to 150°C ea 9711T 

*Order Temp-block and Heating Blocks separately. 


Looking for gas sampling 

bags and other gas 

sampling accessories? 


4961 

Vacuum Bottles with Septum Ports 

• Prepare gaseous calibration blends 

• Easy sampling with a syringe 

• Measure volatiles in packaging materials 

• Prepare coated packings 

• Wide-mouth accessibility 

Vacuum Bottles with Septum Ports 


170cc, 2" Mouth, 2" Diameter, 5.5" Height ea 8089 

390cc, 2" Mouth, 2" Diameter, 6.5" Height ea 8090 

770cc, 3" Mouth, 3" Diameter, 10" Height ea 8091 



Replacement Parts for Vacuum Bottles 

Septa, Red Silicone, 6mm o.d. x 8mm 12/pk 7000 

Septa-Out (Removal Tool) ea 6412 

Replacement O-rings, 2" ea 8087 

Replacement O-rings, 3" ea 8088 

Diazomethane Generators 

Diazomethane is used to methylate acidic 

compounds for analysis by gas chromatography. 

It offers the advantages of clean and quantitative 

reactions but it is highly toxic and explosive. 

Instructions for use are included with each unit. 

Note: Always use appropriate safety precautions 

when handling and preparing diazomethane and 

its solutions. 

4965 

6647 

4664 

Diazomethane Generators 


Diazomethane Generators (Milli-Mole Size) ea 9559 

Replacement PTFE/Silicone Septa 72 95242 

4963 





vials 




Adjustable Hand Crimper 

• Adjustable block corrects for differences in septum 

thicknesses 

The Adjustable Crimper has an adjustable pressure block 

in the crimper head that allows crimping of liners with a 

thickness range of 0.25 to 3.0mm. The unit looks like the 

standard hand crimper but it is supplied with an allen wrench 

and special instructions for making adjustments. 

Adjustable Crimper Instructions 

Adjustment Method: 

1. Hold crimper with jaws up, 

notice hex socket screw. 

2. Insert hex wrench into 

screw socket. 

3. For looser septa, turn screw 

clockwise 1/8" turn. For tighter 

septa, turn screw counter 

clockwise 1/8" turn. 

4953 

The crimper has an adjustment feature which will allow it to be 

used with a variety of septa, discs, stoppers or lined caps. The 

user may adjust the stroke or movement for each application 

as needed. 

4948 

Hand Crimper 

• Permits minor handle pressure adjustments 

• Works best for seals with 40–50mil liners 

tech tip 

What’s special about the adjustable hand crimpers? 

The new design has an adjustable pressure block that corrects 

for differences in septum thickness and provides a tighter seal. 

Crimping is accomplished by placing the crimper head over the 

aluminum seal and squeezing the handle. 

4947 

Hand Decapper 

• Removes crimped seals from vials 

4329 

Plier Decapper 

• Inexpensive tool to remove sealed caps 

3309 

4949 


Volume Description Qty Part No. 

Adjustable Hand Crimpers for Crimp Top Vials 




Hand Crimpers for Crimp Top Vials 





Hand Decappers for Crimp Top Vials 





Plier Decappers for Crimp Top Vials 





vials 


383


Color-Coded PEEK Tubing 

• Chemically inert and biocompatible 

• High strength polymer replaces stainless steel 

• Easy to cut with razor blade or tubing cutters 

PEEK tubing is the ideal metal-free tubing. Its mechanical 

strength, chemical resistance, and biocompatibility make 

it appropriate for a wide variety of high pressure LC 

applications. Tubing is color-coded to indicate i.d. 

PEEK Tubing Specifications 

Material: 

PEEK 


Maximum Pressure: Varies by Size, See Price Block 

Typical Use: 


5535 

Striped PEEK Tubing 

• No dye contacts fluid path 

Striped PEEK Tubing 

Maximum 

Pressure 

(psig) 

Striped 

Part No. 

Color o.d. i.d. 

Length 

Red 1/16" 0.005" 7,000 10' (3.05m) 35714 

50' (15.25m) 35715 

Yellow 1/16" 0.007" 7,000 10' (3.05m) 35712 

50' (15.25m) 35713 

Blue 1/16" 0.010" 5,000 10' (3.05m) 35702 

50' (15.25m) 35703 

Orange 1/16" 0.020" 5,000 10' (3.05m) 35708 

50' (15.25m) 35709 

Green 1/16" 0.030" 4,000 10' (3.05m) 35710 

50' (15.25m) 35711 

Grey 1/16" 0.040" 3,000 10' (3.05m) 35705 

50' (15.25m) 35707 

Black 1/16" 0.055" 1,000 10' (3.05m) 35741 

50' (15.25m) 35742 

0753A 

Solid Color PEEK Tubing 

• Entire tube is colored for faster 

identification 

Solid Color PEEK Tubing 

Maximum 

Pressure 

(psig) 

Solid 

Part No. 


Length 

Red 1/16" 0.005" 7,000 10' (3.05m) 35720 

50' (15.25m) 35721 

Yellow 1/16" 0.007" 7,000 10' (3.05m) 35722 

50' (15.25m) 35723 

Blue 1/16" 0.010" 5,000 10' (3.05m) 35728 

50' (15.25m) 35729 

Orange 1/16" 0.020" 5,000 10' (3.05m) 35726 

50' (15.25m) 35727 

Green 1/16" 0.030" 4,000 10' (3.05m) 35724 

50' (15.25m) 35725 

Natural 0.071" 0.030" 4,000 10' (3.05m) 35776 

50' (15.25m) 35778 

1/8" 0.062" 5,000 10' (3.05m) 35716 

50' (15.25m) 35717 

1/8" 0.080" 3,000 10' (3.05m) 35718 

50' (15.25m) 35719 

0753B 








See page 391 for the 

Clean-cut tubing cutter 

designed to make clean 

square cuts on PEEK tubing. 

tubing 

6673 

5377 



Flex-Connect PEEK Tubing 

• Self-adjusting length 

• Will not bend or kink 

• PEEK construction is 

biocompatible and inert 

Flex-Connect is ideal for 

modular LC systems, giving 

you the freedom to move 

components without breaking 

connections. The coiled format 

springs back to keep excess 

tubing out of your way. Each piece has 6" of straight tubing 

at each end and comes with two 1-piece Hex-Head Fittings. 

Tubing is color-coded to indicate i.d. 

5527 

Flex-Connect Tubing stretches to make connections easier. 

Custom Flex-Connect Tubing 

Description 

Part No. 

Custom Flex-Connect Tubing* C-35800 

*Please specify the following when ordering: color coding (solid or striped), i.d. 

(0.005"–0.030"), and extended length (cm, 800cm max.). 

6264 

Striped Flex-Connect Tubing 

• No dye contacts fluid path 

Striped Flex-Connect PEEK Tubing 


Max. Pressure 

(psig) Size Range* 

Striped 

Part No. 

Red 1/16" 0.005" 7,000 1–5cm 35851 

2–10cm 35852 

3–15cm 35853 

5–25cm 35854 

10–50cm 35855 

15–75cm 35856 

Yellow 1/16" 0.007" 7,000 1–5cm 35861 

2–10cm 35862 

3–15cm 35863 

5–25cm 35864 

10–50cm 35865 

15–75cm 35866 

Blue 1/16" 0.010" 5,000 1–5cm 35871 

2–10cm 35872 

3–15cm 35873 

5–25cm 35874 

10–50cm 35875 

15–75cm 35876 

Orange 1/16" 0.020" 4,000 1–5cm 35881 

2–10cm 35882 

3–15cm 35883 

5–25cm 35884 

10–50cm 35885 

15–75cm 35886 

Green 1/16" 0.030" 4,000 1–5cm 35891 

2–10cm 35892 

3–15cm 35893 

5–25cm 35894 

10–50cm 35895 

15–75cm 35896 

*Size range indicates where coil is completely retracted and pulled to a comfortable 

distance. Excludes 6" straight piece on each end. 

0753A 

Solid Color Flex-Connect Tubing 

• Entire tube is colored for fast 

identification 

Solid Color Flex-Connect PEEK Tubing 


Max. Pressure 

(psig) Size Range* 

Solid 

Part No. 

Red 1/16" 0.005" 7,000 1–5cm 35801 

2–10cm 35802 

3–15cm 35803 

5–25cm 35804 

10–50cm 35805 

15–75cm 35806 

Yellow 1/16" 0.007" 7,000 1–5cm 35811 

2–10cm 35812 

3–15cm 35813 

5–25cm 35814 

10–50cm 35815 

15–75cm 35816 

Blue 1/16" 0.010" 5,000 1–5cm 35821 

2–10cm 35822 

3–15cm 35823 

5–25cm 35824 

10–50cm 35825 

15–75cm 35826 

Orange 1/16" 0.020" 4,000 1–5cm 35831 

2–10cm 35832 

3–15cm 35833 

5–25cm 35834 

10–50cm 35835 

15–75cm 35836 

Green 1/16" 0.030" 4,000 1–5cm 35841 

2–10cm 35842 

3–15cm 35843 

5–25cm 35844 

10–50cm 35845 

15–75cm 35846 

Natural 1/8" 0.062" 2,500 5–50cm 35807 

10–100cm 35808 

20–200cm 35809 

5–50cm 35827 

10–100cm 35828 

20–200cm 35829 

*Size range indicates where coil is completely retracted and pulled to a comfortable 

distance. Excludes 6" straight piece on each end. 

0753B 

tubing 




Hi-EFF Tubing 

Hi-EFF grade stainless steel tubing is especially tempered 

for easy bending and is washed with acetone to remove any 

residual materials. Type 316 is recommended for plumbing 

LC systems. 


This Stainless Steel Tubing is the same high quality as 

the Hi-EFF grade, but has not been washed with acetone. 

Type 316 is recommended for LC use. 

5562 


Looking for tubing cutters? 


Hi-EFF and Standard Stainless Steel Tubing Specifications 

Material: 



Maximum Pressure: Varies according to i.d. 

Typical Use: 



10ft (3m)* 

Part No. 

25ft (7.5m)* 

Part No. 

50ft (15m)* Coil 

Part No. 

200ft Coil 

Part No. 

o.d. 

i.d. 

Hi-EFF Type 316 Stainless Steel 1/8" (3.18mm) 0.085" (2.16mm) 5141338 5141342 30109 — 

1/4" (6.35mm) 0.210" (5.33mm) 5141337 5141343 30309 — 

Type 304 Stainless Steel 1/8" (3.18mm) 0.085" (2.16mm) 5141336 5141344 30106 — 

1/4" (6.35mm) 0.210" (5.33mm) 5141335 5141345 30306 — 

Standard Type 316 Stainless Steel 1/32" (0.79mm) 0.007" (0.18mm) 5141387 5141320 81951 — 

1/16" (1.59mm) 0.004" (0.10mm) 5141386 5141321 30212 — 

0.007" (0.18mm) 5141385 5141322 30142 — 

0.010" (0.25mm) 5141384 5141323 3005 30052 

0.020" (0.51mm) 5141383 5141324 3002 300220 

0.030" (0.76mm) 5141382 5141325 3000 300020 

0.040" (1.02mm) 5141381 5141326 3003 — 

0.050" (1.27mm) 5141380 5141327 3004 — 

Type 304 Stainless Steel 1/8" (3.18mm) 0.085 (2.16mm) 5141346 5141410 3010 30108 

1/16" (4.76mm) 0.147" (3.73mm) 5141332 5141413 3020 — 

1/4" (6.35mm) 0.210" (5.33mm) 5141333 5141412 3030 — 

1/8" (9.53mm) 0.305" (7.75mm) 5141334 5141411 3032 † — 

*Meters based on closest estimate; feet being exact. †40' coil. 

tubing 

EDM-Cut Stainless Steel Tubing 

• Highly polished, burr-free ends 

• Ultra-low volume connections 

• Ultrasonically cleaned and passivated for maximum inertness 

• Cut using an Electronic Discharge Machine (EDM) 

• Color-coded caps identify internal diameter 

EDM-Cut Stainless Steel Tubing Specifications 

Material: 

316 Grade Stainless Steel 



Typical Use: 


EDM Cut 

0743 0744 

File Cut 

Abrasive Cut 

0745 

EDM-Cut Stainless Steel Tubing 

Cap Color i.d. (10/pk) Length Part No. 

Red 0.005" 4cm 97001 

5cm 97006 

10cm 97011 

20cm 97016 

30cm 97021 

Black 0.007" 5cm 97026 

10cm 97031 

20cm 97036 

30cm 97041 

Blue 0.010" 5cm 97046 

10cm 97056 

20cm 97061 

30cm 97066 

Green 0.020" 5cm 97076 

10cm 97081 

20cm 97086 

30cm 97091 

EDM-Cut Stainless Steel Tubing Kits 

6263 

EDM-Cut Stainless Steel Tubing Kits 

Description Tubing i.d. Part No. 

Standard Tubing Kit—Includes 10 pieces 5cm and 10cm tubing 0.005" 97132 

0.007" 97134 

0.010" 97136 

0.020" 97138 

0.030" 97141 

Deluxe Tubing Kit—10 pieces—5cm x 0.010, 10cm x 0.010, 5cm x 0.020, 10cm x 0.020 

5 pieces—2cm x 0.010, 2cm x 0.020 

2 pieces—5cm x 0.030, 10cm x 0.030, 20cm x 0.030 

Varies 97131 

386 



Low-Pressure Tubing 

Tefzel ® Tubing 

• Higher pressure rating 

compared to PTFE 

tubing 

Color-Coded 

PTFE Tubing 

• Color-coded for easy 

identification of i.d. 

5530 

5532 

Tefzel ® Tubing Specifications 

Material: Tefzel ® 



Typical Use: 

Varies by Size, See Price Block 


Tefzel ® Tubing 

o.d. i.d. Length psig Part No. 

1/16" 0.007" 10' 4000 35681 

1/16" 0.007" 50' 4000 35682 

1/16" 0.010" 10' 3600 35685 

1/16" 0.010" 50' 3600 35686 

1/16" 0.020" 10' 3200 35699 

1/16" 0.020" 50' 3200 35672 

1/16" 0.030" 10' 2500 35701 

1/16" 0.030" 50' 2500 35673 

Color-Coded PTFE Tubing Specifications 

Material: 

PTFE 



Typical Use: 



Color-Coded PTFE Tubing 

Color o.d. i.d. Length psig Part No. 

Blue 1/16" 0.010" 10' 1500 35660 

1/16" 0.010" 50' 1500 35661 

Orange 1/16" 0.020" 10' 1200 35662 

1/16" 0.020" 50' 1200 35663 

Green 1/16" 0.030" 10' 900 35664 

1/16" 0.030" 50' 900 35665 

PTFE Tubing 

• Maximum inertness 

PTFE Tubing Specifications 

Material: 

PTFE 



Typical Use: 


Low Pressure Plumbing 

PTFE Tubing 

o.d. i.d. Length psig Part No. 

1/16" 0.007" 10' 1600 35677 

1/16" 0.007" 50' 1600 35678 

1/16" 0.010" 10' 1500 20064 

1/16" 0.010" 50' 1500 35669 

1/16" 0.020" 10' 1200 20033 

1/16" 0.020" 50' 1200 35668 

1/16" 0.030" 10' 900 20031 

1/16" 0.030" 50' 900 35670 

1/16" 0.040" 10' 600 20106 

1/16" 0.040" 50' 600 3132 

1/8" 0.063" 10' 900 20063 

1/8" 0.063" 50' 900 35671 

1/8" 0.100" 10' 300 20096 

1/8" 0.100" 50' 300 3134 

0.160" 0.125" 10' 300 35667 

0.160" 0.125" 50' 300 35674 

1/4" 0.125" 50' 900 3136 

5531 

Low Permeation 

Tubing 

• Eliminates regassing 

without sacrificing 

inertness 

• Flexible, convenient, 

and versatile 

• Easy to install and use 

This low-pressure tubing has a double wall to prevent mobile 

phase regassing. The inert inner PTFE wall carries the mobile 

phase. The non-wetted outer wall is made from a translucent, 

flexible polymer with extremely low gas permeability. This low 

permeation tubing cuts regassing rates to negligible levels, 

while preserving PTFE’s chemical resistance, visibility, and 

handling properties. Use with low permeation fittings kit. 

Low Permeation Tubing Specifications 

Material: 

PTFE, Proprietary Non-Wetted Outer Layer 



Typical Use: 

900psig 


Low Permeation Tubing 

o.d. i.d. Length Part No. 

1/8" 1.5mm 10' 47100 

1/8" 1.5mm 50' 47102 

Low Permeation Fittings Kit 

Description 

Part No. 

1/4-28 Low Permeation Fittings Kit* 47120 

*Includes 10 nuts, 10 ferrules, 10 inserts. 

5533 

tubing 




AT Steel—Activity Tested Steel Tubing 

Delivers the Strength of Stainless Steel and the Inertness of Deactivated Fused Silica 

• Flexible, and strong 

• Maximum temperature limit of 340°C–350°C 

A combination of chemical vapor deposition techniques and silicone chemistries transform durable 

stainless steel tubing into a chromatographically inert material. AT Steel activity tested steel tubing 

is suitable for sample loops, transfer lines, capillary, and packed GC columns. 

AT Steel may be cut with standard tubing cutters or high speed wheel cutters and can be rinsed with 

common solvents to remove particulates and contamination that have built up during use. To ensure a 

truly inert pathway, use AT Steel treated fittings. Sold separately below. 

5105 

AT Steel Tubing 

i.d. 6ft Length Part No. 25ft Length Part No. 50ft Length Part No. 100ft Length Part No. 


0.021" 11085 11086 11087 11088 


0.010" 11060 11061 11062 11063 

0.020" 11064 11065 11066 11067 

0.030" 11068 11069 11070 11071 

0.040" 11072 11073 11074 — 


0.085" 11076 11077 11078 — 


0.210" 11092 11093 — — 

AT Steel Unions 

Description 

Part No. 

1/8"–1/16" Reducing Union 11080 

1/16" Union 11081 

1/8" Union 11082 

AT Steel Tees 

Description 

Part No. 

1/16" Tee 11083 

1/8" Tee 11084 

Fittings are Parker-Hannifin ® A-Lok ® fittings with two-piece ferrules that have been 

chemically modified for improved inertness. 

Straight Stainless Steel Tubing 

• Type 304 Stainless Steel 

• 6ft or 10ft lengths 

Straight Stainless Steel Tubing 

o.d. i.d. Length* Qty. Part No. 

1/8" (3.18mm) 0.085" (2.16mm) 6ft 10 30106ST 

1/8" (3.18mm) 0.085" (2.16mm) 10ft 10 301010ST 

1/4" (6.35mm) 0.210" (5.33mm) 6ft 10 30306ST 

1/4" (6.35mm) 0.210" (5.33mm) 10ft 10 303010ST 

*10ft lengths must ship via motor freight. 

5097 

PTFE-Coated Stainless Steel Tubing 

• Sturdy SS tube with 0.001" thick 

PTFE coating 

• Must be preconditioned at 250°C for 

three hours 

PTFE-Coated Stainless Steel Tubing 


1/8" (3.18mm) 0.085" (2.16mm) per ft* 31421 

1/8" (3.18mm) 0.085" (2.16mm) 50ft 3142 

1/4" (6.35mm) 0.210" (5.33mm) per ft* 31441 

1/4" (6.35mm) 0.210" (5.33mm) 50ft 3144 

*Minimum tubing order is 3ft. 

5103 

tubing 

tech tip 

Choosing a tubing material for GC analysis. 

A number of options are available for packed column tubing. The most inert material is glass, which should be used for active compounds. 

Glass-lined tubing and AT Steel provide the inert surface of glass combined with the mechanical strength of a metal column. 

Metal tubing provides an economical and rugged column for suitable application. Passivated nickel tubing can frequently be used with 

active compounds such as phenols and amines. Stainless steel tubing is recommended for hydrocarbon, fixed gas, and solvent analyses 

where column inertness is less of a concern. 

PTFE tubing is extremely inert, but due to temperature limitations and poor column efficiency, PTFE is generally only recommended for 

the analysis of corrosive gases which are too reactive for glass. 

388 



Glass-Lined Stainless Steel 

Tubing (GLT) 

• Flow lines for corrosives 

• GC/MS interfaces 

PTFE-Lined Stainless Steel Tubing 

• Ideal for use with reactive chemicals and 

for trace quantities of water 

• Order PTFE ferrules to grip SS and PTFE 

portions of tubing below 

• Capillary column connections 5101 5102 

Glass-Lined Stainless Steel Tubing 


1/16" (1.59mm) 0.3mm 180cm 3137 

1/16" (1.59mm) 0.5mm 180cm 3164 

1/16" (1.59mm) 0.7mm 180cm 3139 

1/8" (3.18mm) 0.75mm 180cm 3166 

1/8" (3.18mm) 1.0mm 180cm 3168 

1/8" (3.18mm) 1.5mm 180cm 3170 

1/8" (3.18mm) 1.8mm 180cm 3141 

1/4" (6.35mm) 4.0mm 180cm 3149 • Maximum 

Inertness 

Metal Tubing 

Aluminum Tubing 

• Economical choice for GC gas lines 

Aluminum Tubing 


1/8" (3.18mm) 0.065" (1.65mm) 50ft 3090 

3/16" (4.76mm) 0.128" (3.25mm) 50ft 3100 

1/4" (6.35mm) 0.190" (4.83mm) 50ft 3110 

3/8" (9.53mm) 0.315" (8.00mm) 50ft 3120 

Copper Tubing 

• Economical choice for GC gas lines 

5106 

PTFE-Lined Stainless Steel Tubing 

o.d. i.d. Length Qty. Part No. 

1/8" (3.18mm) 0.070" (1.78mm) 6ft 1 3152 

1/8" (3.18mm) 0.070" (1.78mm) 10ft 1 3154 

1/4" (6.35mm) 0.180" (4.57mm) 6ft 1 3156 

Polymeric Tubing 

PFA (Perfluoroalkoxy) Tubing 

PFA FEP PTFE Polypropylene 

PFA (Perfluoroalkoxy) Tubing 


1/16" (1.59mm) 0.030" (0.76mm) 25ft 45734 

1/8" (3.18mm) 0.062" (1.57mm) 25ft 45735 

1/4" (6.35mm) 0.156" (3.96mm) 25ft 45736 

FEP (Fluorinated Ethylene Polypropylene) 

Tubing 

FEP (Fluorinated Ethylene Polypropylene) Tubing 


1/16" (1.59mm) 0.030" (0.76mm) 25ft 45739 

1/8" (3.18mm) 0.062" (1.57mm) 25ft 45740 

1/4" (6.35mm) 0.156" (3.96mm) 25ft 45741 

5104a 

Copper Tubing 


1/8" (3.18mm) 0.065" (1.65mm) 50ft 3040 

3/16" (4.76mm) 0.128" (3.25mm) 50ft 30509 

1/4" (6.35mm) 0.190" (4.83mm) 50ft 30609 

3/8" (9.53mm) 0.315" (8.00mm) 50ft 30709 

Nickel Tubing 

• Pure Nickel 200, more inert than 

stainless steel 

• Acid washed and rinsed for 

additional inertness 

Nickel Tubing 

o.d. i.d. Part No. 

50-Foot Coil 

1/16" (1.59mm) 0.010" (0.25mm) 3083 

1/16" (1.59mm) 0.020" (0.51mm) 3131 

1/16" (1.59mm) 0.040" (1.02mm) 3085 

1/8" (3.18mm) 0.083" (2.11mm) 3080 

1/4" (6.35mm) 0.210" (5.33mm) 3081 

200-Foot Coil 

1/16" (1.59mm) 0.010" (0.25mm) 30832 

1/16" (1.59mm) 0.020" (0.51mm) 31312 

5100 

5099 

Polypropylene/Polyethylene Tubing 

Polypropylene/polyethylene Tubing 

o.d. i.d. Type Length Part No. 

1/8" (3.18mm) 0.080" (2.03mm) PP 50ft 3203 

1/8" (3.18mm) 0.080" (2.03mm) PP 100ft 3201 

1/4" (6.35mm) 0.125" (3.17mm) PE 50ft 3207 

1/4" (6.35mm) 0.125" (3.17mm) PE 100ft 3205 


Need fittings? 

See pages 282–285 for a complete selection of 

GC fittings. 

tubing 



PEEKsil Tubing 

• Inert fused silica bonded to PEEK 

• Square cut and polished tubing ends 

for zero dead volume connections 

• Ideal for plumbing micro, capillary 

and nano LC systems 

SGE’s PEEKsil tubing maintains the 

easy handling and convenience of PEEK 

tubing, but offers the tight i.d. tolerances 

that can only be offered with fused silica. 

Each PEEKsil tubing end is cut to an exact 90° angle 

and polished smooth to ensure a true zero dead volume 

connection. Tubing is color-coded to identify i.d. It is the ideal 

choice for plumbing capillary and micro LC systems where 

conventional 1/16" or 1/32" fittings are used. 

6388 


• Tight size tolerances for 

maximum reproducibility 

• Easy to cut to desired 

length 

High quality fused silica tubing 

is commonly used to plumb 

capillary LC systems due to 

the size availability and extremely tight tolerances. All tubing 

below has 360µm outer diameter. 

Fused Silica Capillary Tubing Specifications 

Material: 

Polyimide-Coated Fused Silica 

Maximum Temperature: 350ºC 


Typical Use: 

Limited by connection system 

Plumbing Capillary LC Systems 

6625 

tubing 

PEEKsil Tubing Specifications 

Material: 

Fused Silica-Lined PEEK 


Typical Use: 

Plumbing Capillary LC Systems 

Maximum Pressure: 15,000psig 

PEEKsil Tubing, 1/16 o.d. 

10cm 

5/pk 

Part No. 

15cm 

5/pk 

Part No. 

Length 

20cm 

5/pk 

Part No. 

50cm 

2/pk 

Part No. 

Color i.d. (mm) 

Orange 0.025 226 227 228 229 

Natural 0.050 252 254 253 250 

Black 0.075 291 292 293 294 

Red 0.100 302 304 303 300 

Purple 0.150 231 232 233 234 

Yellow 0.175 352 354 353 350 

Blue 0.200 403 406 404 401 

Grey 0.300 416 417 418 419 

6389 


i.d. 

(mm) 

1 meter 

Part No. 

15 meter 

Part No. 

0.025 991100 — 

0.050 602035 — 

0.100 602036 — 

0.180 19603 602014* 

0.250 602600 602615 

0.320 603600 603615 

0.450 19602 — 

*Length for 0.180mm is 10 meter. 

Micro-Tubing 

• Consistent square cut for 

low volume connections 

Use micro-tubing for 

connecting fused silica 

capillaries to ensure proper 

low volume connections with 

your micro LC applications. 

Micro Tubing 

Color Reduction Part No. 

Red 1/16" to 127µm o.d. 3109278 

Yellow 1/16" to 178µm o.d. 3109280 

Natural 1/16" to 229µm o.d. 3109282 

Blue 1/16" to 280µm o.d. 3109283 

Orange 1/16" to 330µm o.d. 3109284 

Red 1/16" to 360µm o.d. 3109299 

Green 1/16" to 380µm o.d. 3109285 

Black 1/16" to 457µm o.d. 3109286 

Natural 1/16" to 533µm o.d. 3109287 

new 

7418 

7419 

390 



Tubing Cutters 

Rotary Tubing Cutter 

Heavy-duty Tubing Cutter 

• Inexpensive, well-built tool 

• Cuts 1/8" to 1-1/8" o.d. tubing 

• Works with metal tubing 5113 

Plier-type Tubing Cutters 

best 

seller 

• Cuts 1/32" to 1/8" o.d. tubing 

burr-free 

• Leaves tubing i.d. open 

• Works with metal and glass-lined tubing 

• Cuts 1/16" o.d. tubing 

• Ideal for cutting tubing in tight places 

• Cuts metal or plastic tubing 

5110 

Tubing Accessories 

Flex-wrap Tubing 

• Holds parallel tubing 

or wiring together 

• Ideal for gradient pumps 

or purging-gas lines 


100% PTFE 

Flex-Wrap Tubing 

1/8"–1/2" 

Part No. 

3/16"–1" 

Part No. 

1/4"–2" 

Part No. 

Color 

Blue 35913 35923 35933 

Orange 35914 35924 35934 

Black 35916 35926 35936 

White 35917 35927 35937 

2357 

Ceramic Fused Silica Cutter 

• Cleanly cuts fused silica tubing 

• Small enough to keep handy in 

your pocket 

• Just score and snap 

3306 

Cable Turtles 

• Neat, easy way to organize messy cables 

• Patented “Turtle” stores and conceals excess cords 

Electric Tubing Cutter 

• Cut 1/16", 1/8", and 1/4" o.d. tubings 

with internal diameters as small 

as 0.008" 

• Requires no lubricant or cutting fluid 

• Assures square and burr-free cuts 

Clean-cut Tubing Cutter 

• Use with PTFE, Tefzel ® , 

Polyolefin, PEEK 

• Make clean, square cuts without 

deforming inner diameters 

• Cuts 1/32" to 1/8" o.d. 

Plastic Tubing Cutter 

• Ideal for PEEK, Tefzel ® , and other 

plastic tubing 

• Cuts any size up to 1/4" o.d. 

Tubing Cutters 


Rotary Tubing Cutter ea 3165 

Replacement Cutting Wheels 3 3167 

Heavy-Duty Tubing Cutter ea 13995 

Replacement Cutting Wheels 3 13981 

Plier-Type Tubing Cutter ea 3169 

Ceramic Fused Silica Cutter 5 3194 

Electric Tubing Cutter 110–120/220–240VAC, 50/60Hz ea 10-0200 


Dressing Tool for 1/8" and 1/4" o.d. Tubing ea 10-0118 

Replacement Cutting Wheels 3 10-0124 

Replacement Dressing Tool for 1/16" o.d. Tubing ea 10-0116 

Clean-Cut Tubing Cutter ea 35902 

Replacement Blade ea 35903 

Plastic Tubing Cutter ea 3206 

Replacement Blade ea 3214 

4502 

6281 

5377 

5376 

Flexible rubber cup opens to let you coil extra cables, cords, 

tubing, or wires. Small Cable Turtle will holds up to 1 meter 

of standard appliance cord, and 3 meters of telephone wire. 

Large Cable Turtle holds up to 1 meter of thick instrument 

cord, 2 meters of standard appliance cord, or 6 meters of 

telephone wire. 

Cable Turtles 


Small 

Red 5/pk 5274 

Blue 5/pk 5276 

Black 5/pk 5278 

Grey 5/pk 5280 

Large 

Red 4/pk 5282 

Blue 4/pk 5284 

Black 4/pk 5286 

Grey 4/pk 5288 

4174 

tubing 



Organizers 

HPLC Columns and 

Accessories Organizers 

• Holds 10 HPLC columns 

• 17 compartments 

• PVC construction 

• 12" H x 24" W x 6" D 

(30.48cm H x 60.96cm W x 15.24cm D) 

HPLC Columns Organizers 

• 30 HPLC columns 

• Wall-mounts with two screws 

• PVC construction with acrylic 

doors 

• 17-3/8" H x 15" W x 2-7/8" D 

(44.18cm H x 38.1cm W x 

7.3cm D) 

Plastic Box with Removable 

Dividers 

• 13 removable dividers 

• Ideal for fittings and bore-cut 

tubing 

5507 

best 

seller 

5508 

GC Column and 

Accessory Organizers 

• 5-Column Organizer: 

13" H x 10.5" W x 14" L 

(33cm H x 26.5cm W x 35.5cm L) 

• 10-Column Organizer: 

12.75" H x 10.5" W x 25" L 

(32cm H x 26.5cm W x 63.5cm L) 

Organizer Box 

• 9-compartment organizer 

includes eight 1-3/8" x 1-5/8" 

(3.5cm x 4.13cm) slots and 

one 1-1/8" x 3-1/4" (2.8cm x 

8.2cm) slot 

• 18-compartment organizer 

includes twelve 2" x 2" (5cm x 

5cm) slots and six 2" x 4-3/16" 

(5cm x 10.6cm) slots 

5688 

5687 

5772 

Lab Organizer with Adjustable Bins 

• 14 adjustable dividers, 4 shelves 

• PVC construction 

• 20" H x 24" W x 10.5" D 

(50.8cm H x 60.9cm W x 26.7cm D) 

6348 

Organizers 

Description 

Part No. 

HPLC Supplies Organizer 5238 

HPLC Column Storage Unit 5240 

Plastic Storage Box with Removable Dividers 803880 

Lab Organizer with Adjustable Bins 5242 

GC Column and Accessory Organizers 

GC Column Organizer, 5-Column 6390 

GC Column Organizer, 10-Column 6392 

Organizer Box 

9-Compartment Box 6264 

18-Compartment Box 6266 

5509 

tools & organizers 


Looking for vial organizers 

and storage racks? 


3428 4954 

392 



Tools 

Magnifiers 

• For inspecting capillary column ends 

9-Way Wrench 

• For use with gas cylinders 

5178 

20X Magnifier 

Deluxe Torch Kit 

5325 2156 

Lighted Magnifying Glass 

• Ideal for sealing the ends of 

capillary columns 

• Kit contains the torch, six Micronox 

cylinders, three butane cylinders, six silver 

soldering rods, flux tube, two flame tips, 

one butane tip, one spark lighter, and 

complete instructions 

5181 

Miniature Files, Fine Cut 

• Set includes six differentshaped, 

5-1/2" long files 

• Made of hard chrome steel 

suitable for smoothing and 

deburring 

Miniature Reamers 

• Set includes six different sizes: 

0.028"/0.018", 0.040"/0.026", 

0.048"/0.032", 0.080"/0.050", 

0.085"/0.053", 0.104"/0.062" 

6279 

5324 

Capillary Tool Kit 

• Kit includes diamond scriber, 

two open end wrenches, PTFEcoated 

forceps, ruler, ferrule 

pick, 10' high temperature string, 

20X magnifier, and pipe cleaner 

23-Piece Tool Kit in Zippered Case 

• Kit includes seven screwdrivers, adjustable wrench, two 

pliers, wire stripper, knife, alignment 

tool, stainless rule, hex key set, 

scissors, two flexible files, burnisher, 

miniature soldering iron, solder aid, 

coil of solder, and desoldering braid 

Adjustable Wrench 

• Chrome-Plated 

Open-End Wrench 

• Chrome-Plated 

Handy-Lok Fitting Wrench 

• Ideal for connections on 

injection valves 5641 

5179 

5323 

5322 

5321 

Flame Checker 

• Pivoting mirror head fogs 

when flame is lit 

Tools 


Magnifier, 20X ea 3163 

Magnifier, Lighted (includes battery) ea 3196 

Deluxe Torch Kit 14606 

Micronox Cylinders 6/pk 14602 

Butane Cylinders 6/pk 14603 

Piercing Knob, Butane ea 14604 

Piercing Knob, Micronox ea 14605 

Capillary Tool Kit ea 16879 

23-Piece Tool Kit and Case ea 16533 

Adjustable Wrench 

4" Long—1/2" Capacity ea 16549 



Open-End Wrench 

1/4" x 5/16"—Fits 1/16" Fitting Nuts ea 1998 

7/16" x 1/2"—Fits 1/8" and 3/16" Fitting Nuts ea 1999 

9/16" x 5/8"—Fits 1/4" and 3/8" Fitting Nuts ea 2000 

Handy-Lok Fitting Wrench ea 35761 

9-Way Cylinder Wrench ea 412933 

Miniature Files, Fine Cut 6/pk 7237 

Miniature Reamers 6/pk 7330 

Flame Checker ea 7047 

5715 

tools and organizers 


Applications 

HPLC Applications 395–453 

Pharmaceuticals 395–410 

Nutraceuticals/Vitamins 411–418 

Food & Beverage 419–428 

Life Science 429–443 

Environmental 444–447 

Miscellaneous 448–453 

IC Applications 454–461 

Anions 454–458 

Cations 459–460 

Surfactants 461 

GC Applications 462–493 

Drugs 462–467 

Environmental 468–476 

Foods, Flavors & Fragrances 476–481 

Petroleum & Petrochemical 481–485 

Solvents & Chemicals 485–493 

SPE Applications 494–502 

Pharmaceuticals 494 

Nutraceuticals 495–496 

Food & Beverage 497 

Life Science 498 

Environmental 499–502

hplc applications 

Analgesics 

Anti-Inflammatories 

Ibuprofen (U.S.P.) 

1 

2 

3 

4 

1. Naproxen 

2. Acemetacin 

3. Indomethacin 

4. p-Xylene 

8156 10702 

1 

2 


2. Ibuprofen 

pharmaceuticals 

0 2 4 6 8 10 Min. 

Column: Alltech ® Platinum C18, 5µm, 150 x 4.6mm 

(Part No. 32043) 

Mobile Phase: 0.02M KH2PO4:Methanol:Acetonitrile: 

pH4.5 (40:50:10) 



0 

2.5 5 7.5 10 12.5 15 17.5 20 Min. 

Column: GraceSmart C18, 5µm, 150 x 4.6mm 

(Part No. 5138812) 

Mobile Phase: Phosphoric Acid in Water, pH2.5:Acetonitrile (65:35) 




Analgesics 

10124 

1 

Analgesics 

9385 

1 

2 

3 

4 

6 

1. Aspirin 


3. Naproxen 

4. Fenoprofen 

5. Ibuprofen 

6. Diclofenac 

2 

3 4 

1. Aspirin 


3. Naproxen 

4. Fenoprofen 

5 

0 2 

4 6 8 10 Min. 

Column: Alltech ® Alltima C8, 5µm, 150 x 4.6mm (Part No. 88072) 

Mobile Phase: A: 0.05M K2HPO4, pH3.3 B: Methanol 

C: Acetonitrile 


%B: 10 10 30 

%C: 10 10 25 



0 2 4 6 8 10 12 14 Min. 

Column: Alltech ® Prevail Amide, 5µm, 150 x 4.6mm 

(Part No. 88660) 

Mobile Phase: 0.02M K2HPO4, pH7.0:Acetonitrile (75:25) 



2 

Basic Drugs 

Naproxen Enantiomers 

10374 8769 

1 

3 

1. Procainamide 



4. Caffeine 

CH 3 

CHCOH 

4 

H 3 CO 

O 

0 3 6 9 12 15 Min. 6 8 10 12 Min. 

Column: Alltech ® Alltima HP C8, 5µm, 150 x 4.6mm 

Column: Astec Chirobiotic ® V, 250 x 4.6mm (Part No. 11024) 

(Part No. 87751) 

Mobile Phase: 0.1% Triethylammonium Acetate (TEAA), pH7.0: 

Mobile Phase: 0.05M Phosphate Buffer pH3.0:Methanol (80:20) 

Tetrahydrofuran (90:10) 






View our complete searchable chromatogram database at www.discoverysciences.com/chromdb/ 

ww.discoverysciences.com 

395



Analgesics 

1 

2 

0 2 4 6 8 10 Min. 

2 

3 

3 

Analgesics 

1. Solvent front 

2. Acetaminophen (Paracetamol) 

3. Codeine phosphate 

Column: Jones Genesis ® C18, 4µm, 100 x 4.6mm 

(Part No. FM10960E) 

Mobile Phase: Acetonitrile:25mM KH2PO4, pH3.0 (10:90) 



Steroids 

Steroids 

1 




9392 

mAU 

2000 

1750 

1500 

1250 

1000 

750 

500 

250 

0 

0 

1 

Paracetamol Effervescent Tablets on 

Chinese Pharmacopeia Assay 

1 2 3 4 5 6 7 8 9 10 Min. 




2 

3 4 

5 

Corticosteroids 

6 

7 

1. Paracetamol 

10770 



GraceSmart C18 

Column: GraceSmart C18, 5µm, 250 x 4.6mm (Part No. 5138810) 

Mobile Phase: Phosphate Buffer:Methanol (80:20) 



10391 

1. Deoxycorticosterone 

Acetate 


3. Cortisone Acetate 

4. Cortisone 

5. Prednisone 


7. Prednisolone 

0 1 Min. 

Column: Alltech ® Prevail C18, 3µm, 53 x 7mm Rocket 

(Part No. 99279) 

Mobile Phase: Water:Methanol (10:90) 


Detector: UV at 254 

0 

1 2 3 4 5 6 7 8 9 10 11 12 13 Min. 

Column: Alltech ® Alltima HP CN, 5µm, 150 x 4.6mm 

(Part No. 87769) 

Mobile Phase: Hexane:Isopropanol (93.7:6.3) 



3 

Hydrocortisone Acetate Cream 

9463 

Estropipate Sample 

10423 

1 

1. Methyl Paraben 

2. Propyl Paraben 


1. Estrone Sulfate 

2. Piperazine 

1 

2 

2 

0 1 2 3 4 5 Min. 

Column: Alltech ® Prevail C18, 5µm, 150 x 4.6mm (Part No. 99208) 




0 1 2 3 4 5 6 7 8 9 10 11 12 13 Min. 

Column: Alltech ® Alltima CN, 5µm, 250 x 4.6mm (Part No. 88189) 

Mobile Phase: 0.15% Nitric Acid:Acetonitrile (5:95) 



396 



Antidepressants 

Tricyclic Amines 

Alprazolam and Triazolam by the USP XXII Method 

1 

2 

3 

1. Fluphenazine HCl 

2. Chlorpromazine HCl 

3. Phenothiazine HCl 

9105 

1 

2 

1. Triazolam 

2. Alprazolam 

7368 


0 1 

2 

3 

2 

4 5 6 7 8 Min. 

Column: Alltech ® Alltima CN, 3µm, 150 x 4.6mm 

(Part No. 81189) 

Mobile Phase: 0.01M KH2PO4, pH2.6:Acetonitrile (50:50) 



3 

Basic Drugs 




10353 

0 2 4 6 8 10 12 14 16 18 20 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

1 

2 

Alltech ® Alltima Silica, 5µm, 300 x 4.6mm (Custom) 

Acetonitrile:Chloroform:i-Butyl Alcohol:Water:Glacial 

Acetic Acid (85:8:5:2:0.05) 

2.0mL/min 

UV at 254nm 

Tricyclic Antidepressants 

4 


2. Nortryptyline 



10373 

1 

3 

0 1 2 3 4 5 6 7 8 9 Min. 

0 

2 4 6 8 10 12 14 16 18 20 22 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

1. Chlordiazepoxide Degradant 

2. Nitrazepam 

3. Norchlordiazepoxide 

4. Nordiazepam 

3 

2 

5 

4 

1 

Alltech ® Alltima HP C18 Amide, 5µm, 150 x 4.6mm 

(Part No. 87733) 

0.025M Potassium Phosphate Buffer, pH7.0:Methanol 

(15:85) 

1mL/min 

UV at 254nm 

Benzodiazepines and Metabolites 

6 

8691 

5. Chlordiazepoxide 

6. Chlordiazepoxide Degradant 

7. Diazepam 

7 


(Part No. 87751) 

Mobile Phase: A: 0.025M Potassium Phosphate Buffer, pH7.0 

B: Methanol 


%B: 60 75 75 



1 

Universal High Speed Gradient 

2 

3 

4 

5 

8957 



3. Propranolol 



0 2 

4 6 Min. 

Column: Alltech ® Platinum EPS C18, 3µm, 53 x 7mm Rocket 

(Part No. 50573) 

Mobile Phase: 0.05M Ammonium Acetate, pH5.5:Acetonitrile (65:35) 



0 1 2 3 4 5 6 Min. 

Column: Alltech ® Alltima C18, 3µm, 53 x 7mm Rocket 

(Part No. 50605) 

Mobile Phase: A: 0.1% Trifluoroacetic Acid in Water 

B: 0.1% Trifluoroacetic Acid in Acetonitrile 


%B: 10 90 






397




1 

2 

3 

4 

Propanolol 

0 1 2 3 4 5 6 7 8 Min. 

10719 

1. Uracil 

2. Propanolol 


4. Dipropylphthalate 

Column: Grace ® VisionHT C18, 1.5µm, 50 x 2.0mm 

(Part No. 5139605) 

Mobile Phase: 20mM Potassium Phosphate, pH2.5:Methanol (40:60) 




1 

2 

3 

4 

Propanolol 

0 1 2 3 4 5 6 7 8 Min. 

1. Uracil 

2. Propanolol 


4. Dipropylphthalate 

10720 

Column: Grace ® VisionHT C18-P, 1.5µm, 50 x 2.0mm 

(Part No. 5139606) 





1 

2 

3 

Basic Drugs 

Basic Drugs 

4 

5 

6 

1. Amiloride 


3. Chloramphenicol 



6. Trifluoperazine 

Barbiturates 

2 

1 

3 

4 

5 

Barbiturate Mix 

1. Barbital 


3. Talbutal 

4. Amobarbital 

5. Mephobarbital 

10375 

0 1 2 3 4 5 6 7 Min. 

Column: 

GENESIS Jones FM15960E Genesis (C18, ® C18, 4 4µm, 4.6 150 mm i.d. x 4.6mm x 150 mm) 

Flow: 

2.0 mL/min (Part No. FM15960E) 

Mobile Eluent: Phase: Isocratic, Acetonitrile:50mM 35% ACN in mM KH2PO4, 2 POpH2.4 4 , 2.4 (35:65) 

Temp: 40 °C 


Detection: Absorbance, 254 nm 


Peaks: 1. Amiloride, 2. Propranolol, 3. Chloramphenicol, 

4. Carbamazepine, 5. Trimipramine, 6. Trifluoperazine 

0 

2 4 6 8 10 12 14 16 18 20 Min. 


(Part No. 87751) 

Mobile Phase: 0.05M Ammonium Acetate:Acetonitrile (78:22) 





10604 8684 

1 

2 

3 

4 

5 

6 7 


2. Butabarbital 

3. Butalbital 

4. Pentobarbital 



7. Secobarbital 

1 

2 

3 

4 5 6 

7 

8 

1. Barbital 

2. Butalbarbital 

3. Aprobarbital 

4. Talbutal 





0 2 4 6 8 10 12 14 16 18 Min. 

0 1 

2 3 4 Min. 

Column: Alltech ® Alltima HP C18 HiLoad, 5µm, 250 x 4.6mm 

(Part No. 87698) 





(Part No. 50575) 

Mobile Phase: 0.01M Sodium Acetate, pH4.0:Acetonitrile (75:25) 



398 



Barbiturates 

2 

4 

1 3 

5 


1. Primidone 

2. Metharbital 



5. Phenytoin 

10164 



3 

4 

2 

1 

1. Ethotoin 



4. Diazepam 

9394 


0 2 

4 6 8 10 12 Min. 0 2 

4 6 8 Min. 

Column: ZirChrom ® DiamondBond-C18, 3µm, 100 x 4.6mm 

(Part No. DB01-1046) 

Mobile Phase: 0.05M Ammonium Phosphate, pH7.0:Tetrahydrofuran 

(75:25) 



Column: Alltech ® Prevail Phenyl, 5µm, 150 x 4.6mm 

(Part No. 99239) 



%B: 25 60 





10348 10349 

1 

2 

1. Ethotoin 

2. Phenytoin 



3 4 0 2 4 6 8 10 12 14 16 18 20 Min. 

1 

2 

3 

4 

1. Ethotoin 

2. Phenytoin 



0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min. 

Column: Alltech ® Alltima HP EPS C18, 5µm, 150 x 4.6mm 

(Part No. 87715) 





(Part No. 87697) 


Anti-Anginal Drugs 



Anti-Anginals 



2 

3 

1. Uracil 


3. Phenytoin 

1 

2 

Anti-Anginal Drugs 

3 

4 

1. Atenolol 

2. Metoprolol 


4. Diltiazem 

1 

0 1 2 3 4 5 Min. 

0 1 2 3 4 5 Min. 

Column: Jones Genesis ® Phenyl, 4µm, 50 x 4.6mm 

Column: GENESIS FM5980E (Phenyl, 4 µm, 4.6 mm i.d. x 50 mm) 


Flow: 

1.0 mL/min 

Mobile Eluent: Phase: Isocratic, Methanol:Water 30% MeOH in (30:70) water 

Flow Detection: Rate: Absorbance, 1.0mL/min 210 nm 

Detector: 

Peaks: 1. Uracil, UV at 2. 210nm Carbamazepine, 3. Phenytoin 



Column: Column: GENESIS Jones Genesis FM10960E 

® C18, 4µm, 100 x 4.6mm 

(C18, (Part 4 No. µm, FM10960E) 

4.6 mm i.d. x 100 mm) 

Mobile Flow: Phase: 1.0 Acetonitrile:50mM mL/min 

KH2PO4, pH3.0 (40:60) 

Flow Eluent: Rate: Isocratic, 1.0mL/min 40% ACN in 50 mM KH 2 PO 4 , pH 3.0 

Detector: Detection: Absorbance, UV at 254nm 254 nm 

Peaks: 1. Atenolol, 2. Metoprolol, 3. Propranolol, 4. Diltiazem 


399




1 

2 

3 

4 


5 



3. Cyclizine 

4. Chlorcyclizine 

5. Meclizine 


7232 4 

10163 

1 

2 

3 

5 



3. Cyclizine 

4. Pyrrobutamine Phosphate 

5. Meclizine 

0 5 10 15 20 25 Min. 

0 1 

2 

3 

4 

5 

6 Min. 


Mobile Phase: A: 0.05M Potassium Phosphate, pH3.2 



%B: 25 25 70 70 



Column: ZirChrom ® PS, 3µm, 50 x 4.6mm (Part No. ZR09-0546) 

Mobile Phase: A: Acetonitrile 

B: 25mM Hydrochloric Acid 


%B: 95 85 80 40 





Wide-pore reversed-phase columns can have advantages for smallmolecule 

separations. Wide-pore Here a reversed-phase Vydac ® 218TP columns 300Å can C18 have advantages column separated 

for 

a mixture of antihistamines small-molecule in the separations. same time Here as a VYDAC a 90Å 218TP C18 300 column, Å C18 but 

with less organic solvent column in separated the gradient a mixture and of antihistamines better resolution. in the same time 

as a 90 Å C18 column, but with less organic solvent in the gradient 

Column: 

and better resolution. 

Vydac ® C18, 300Å, 5µm, 100 x 4.6mm 

(Part No. 218TP5410) 



Gradient (A): Time: 0 10 

%B: 5 35 

Gradient (B): Time: 0 10 

%B: 10 40 



(a) 

(b) 

1 

2 

1 

2 

3 

3 

4 

4 

5 

5 

VYDAC ® 218TP5410 

300 Å C18 

0 2 4 6 8 10 Min. 

6 

7 

VYDAC ® 201TP5410 

90 Å C18 

6,7 

1. Pheniramine 

2. Doxylamine 

3. Methapyraline 

4. Chlorpheniramine 

5. Orphenadrine 

6. Diphenylpyraline 

7. Promethazine 

1 

2 

OTC Antihistamine and Excipients 

3 

4 5 


2. PEG, Gelatin 

3. Sorbitan 

4. Glycerol 

5. Sorbitol 

10275 

Columns: VYDAC 218TP5410 (300 Å) C18, 5 µm, 4.6 mm i.d. x 100mm 

Flow: 

2.5 mL/min 

Eluent: Estrogens A = 0.1% (v/v) TFA in H 2 O B = 0.1% (v/v) TFA in ACN 

Gradient: a) 5% to 35% B in 10 Estrogens 

min b) 10% to 40% B in 10 min 


Peaks: 1) pheniramine; 2) doxylamine; 3) methapyraline; 

4) chlorpheniramine; Estrogens 5) orphenadrine; 6) diphenylpyraline; 

7) promethazine 

1. Estriol 

2. 17α-Ethylestradiol 

3. Estrone 

4. Diethylstilbestrol 

5. Hexestrol 

6. Product of Diethylstilbestrol 

Decomposition 

1 

2 

3 

4 

5 

6 

400 

0 1 2 3 4 5 6 7 8 9 10 11 Min. 

Column: Alltech ® Prevail Carbohydrate ES, 5µm, 250 x 4.6mm 

(Part No. 35101) 

Mobile Phase: Acetonitrile:0.1% Ammonium Carbonate, pH6.5 (80:20) 




0 1 2 3 4 5 6 7 8 9 Min. 

Column: Vydac ® Denali ® , 5µm, 150 x 4.6mm (Part No. 238DE5415) 

Column: DENALI 238DE5415 (5 µm, 4.6 mm i.d. x 150 mm) 

Mobile Phase: A: Acetonitrile:Water (20:80) 

Flow: 

1.0 mL/min 

Eluent: A = B: 20% Acetonitrile:Water ACN in water (95:5) 

Gradient: B = Time: 95% ACN in 0 water 8 

Gradient: Linear, %B: 40% to 40 90% 90 B over 8 min 

Flow Temp: Rate: Ambient, 1.0mL/min 22 °C 

Detector: 

Detection: Absorbance, UV at 280nm 

nm 

Sample: 20 µL containing 150 ppm of each analyte 

Peaks: 1. Estriol, 2. 17a-Ethylestradiol, 3. Estrone, 

4. Diethylstilbestrol, 5. Hexestrol, 

6. product of Diethylstilbestrol decomposition.


Anesthetics 

1 + 2 

3 

Basic Pharmaceuticals 

1. Procaine 

2. Lidocaine 

3. Tetracaine 

1 2 

Basic Pharmaceuticals 

10737 10738 

3 

1. Procaine 

2. Lidocaine 

3. Tetracaine 


0 0.5 1 1.5 2 2.5 3 Min. 

Column: Grace ® VisionHT C18, 1.5μm, 50 x 2.0mm 

(Part No. 5139605) 




0 0.5 1 1.5 2 2.5 3 Min. 

Column: Grace ® VisionHT C18-P, 1.5μm, 50 x 2.0mm 

(Part No. 5139606) 




Anesthetic Mix 

Anesthetics 

Anesthetics 

4 

10118 

3 

1 

2 

3 

5 

1. p-Aminobenzoic Acid 

2. Benzocaine 

3. Tetracaine 

4. Dibucaine 

5. Butacaine 

1 

2 

4 

5 

6 

1. Procaine 

2. Benzocaine 


4. Lidocaine 

5. Tetracaine 

6. Dibucaine 

0 

2 4 6 8 10 12 Min. 

3 

0 10 20 30 40 50 60 Min. 

Column: Alltech ® Alltima C8, 5µm, 150 x 4.6mm (Part No. 88072) Column: Vydac ® Denali ® , 5µm, 150 x 4.6mm (Part No. 238DE5415) 

Mobile Phase: A: Water B: Methanol C: Acetonitrile 

Mobile Phase: Methanol:50mM K2HPO4 in Water, pH7.0 (60:40) 


Column: Flow Rate: DENALI 238DE5415 1.0mL/min (5 µm, 4.6 mm i.d. x 150 mm) 

%B: 15 45 

Flow: 

Detector: 

1.0 mL/min 

UV at 220nm 

Eluent: Isocratic, 60:40 MeOH:H 2 O containing 

%C: 15 35 

50 mM K 2 HPO 4 , pH 7 


Temp: 30 °C 



Peaks: 1. Procaine, 2. Benzocaine, 3. Mepivacaine, 

4. Lidocaine, 5. Tetracaine, 6. Dibucaine 

Opiates 

Opiates 

10165 

Opiates 

1 

2 

4 

1. Naloxone 

2. Codeine 

3. Ethylmorphine 

4. Oxycodone 

1 

3 

Opiates 

1. Hydromorphone 

2. Oxycodone 

3. Codeine 

4. Ethylmorphine 

5. Nalorphine 

2 

4 

5 

0 1 2 3 4 5 Min. 0 5 10 15 20 25 Min. 

Column: ZirChrom ® DiamondBond-C18, 3µm, 100 x 4.6mm Column: Vydac ® Denali ® , 5µm, 250 x 4.6mm (Part No. 238DE54) 

(Part No. DB01-1046) 

Mobile Column: Phase: DENALI Methanol:20mM 238DE54 (5 µm, K2HPO4 4.6 mm in i.d. Water, x 250 mm) pH7.0 (50:50) 

Mobile Phase: 0.02M Ammonium Phosphate, pH11.0: 

Flow: Rate: 1.0 1.0mL/min 

Tetrahydrofuran (73.5:26.5) 

Detector: 

Eluent: Isocratic, 

UV at 

50:50 

254 

MeOH:H 2 O containing 

20 mM K 2 HPO 4 , pH 7 


Temp: Ambient at 22 °C 



Sample: 10 µL containing 10 µg of each analyte 


Peaks: 1.Hydromorphone, 2. Oxycodone, 3. Codeine, 


4. Ethylmorphine, 5. Nalorphine ww.discoverysciences.com 

401


Anti-Hypertensives 

Antihypertensives 

Basic Drugs 


1 2 

3 

1. Metoprolol 

2. Oxprenolol 


9395 

1 

2 

3 

4 

5 

6 

1. Labetalol 

2. Acebutolol 

3. Oxprenolol 

4. Lidocaine 

5. Alprenolol 


10161 

0 1 2 Min. 

Column: Alltech ® Prevail C18, 3µm, 53 x 7mm (Part No. 99279) 




0 1 2 3 4 5 Min. 

Column: ZirChrom ® PBD, 3µm, 150 x 4.6mm (Part No. ZR03-1546) 

Mobile Phase: 0.01M Triethylamine, 0.05M Tetramethylammonium 

Hydroxide, pH12.2:Acetonitrile (65:35) 



Calcium Channel Blockers 

1 

10356 

Drug Mix 

10354 

2 

3 

4 

1. Diltiazem 

2. Verapamil 

3. Nicardipine 

4. Nifedipine 

2 

3 

1. Codeine 

2. Strychnine 

3. Quinine 

4. Quinidine 

1 

4 

0 1 2 3 4 5 6 7 8 9 10 Min. 


(Part No. 87769) 

Mobile Phase 0.02M KH2PO4, pH3.0:Acetonitrile (70:30) 



0 2 4 6 8 10 12 14 16 18 20 22 24 Min. 

Column: Alltech ® Alltima HP C18 Amide, 5µm, 150 x 4.6mm 

(Part No. 87733) 

Mobile Phase: 0.025M Potassium Phosphate, pH2.8:Methanol (90:10) 



Sedatives 

2 

1 

3 

4 

5 

0 2 4 6 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Sedatives/Hypnotics 

1. Barbital 


3. Butalbital 

4. Oxazepam 

5. Triazolam 

8160 

Alltech ® Platinum C18, 5µm, 150 x 4.6mm 

(Part No. 32043) 

0.05M Ammonium Dihydrogen Orthophosphate, pH4.5: 

Methanol:Acetonitrile (40:50:10) 

1.0mL/min 

UV at 230nm 

mAU 

Statins 

180 

160 

140 

120 

100 

80 

60 

40 

20 

0 

0 

Simvastatin Tablets (U.S.P.) 






2 4 6 8 10 12 14 16 Min. 

10708 


Mobile Phase: 0.39% Sodium Phosphate Monobasic, pH4.5: 




402 



Anti-Asthmatics 

Methylxanthines 

1 2 

3 

4 

5 

8384 8679 


1. Xanthine 



3. 8-Chlorotheophylline 


4. Caffeine 

4. Caffeine 

5. 7-(2-Chloroethyl)theophylline 1 

5. 8-Chlorotheophylline 

6. 7-(2-Chloroethyl)theophylline 

2 

3 4 5 

Theophylline & Metabolites 

6 


0 2 4 6 8 10 Min. 

Column: Alltech ® Platinum EPS C18, 100Å, 5µm, 150 x 4.6mm 

(Part No. 32214) 

Mobile Phase: Water:Acetonitrile:Methanol:Glacial Acetic Acid (60:10:30:1) 



0 1 

2 3 Min. 


(Part No. 50575) 

Mobile Phase: 0.010M Sodium Acetate, pH4.0:Methanol (70:30) 



Amphetamines 

1 2 

3 

MDA and MDMA Analogs 

4 

1. 2,3-MDA 

2. 3,4-MDA 

3. 2,3-MDMA 

4. 3,4-MDMA 

10427 

1 

2 

MDA & MDMA Analogs 

1. 2,3-MDA 

2. 3,4-MDA 

3. 2,3-MDMA 

4. 3,4-MDMA 

10014 

3 4 

0 1 2 3 4 5 6 7 8 9 Min. 

Column: Alltech ® Alltima HP Silica, 3µm, 150 x 4.6mm 

(Part No. 87786) 

Mobile Phase: 1%Ammonium Hydroxide in Methanol:Acetonitrile (75:25) 




0 1 2 3 4 Min. 

Column: Alltech ® Alltima Silica, 3µm, 100 x 4.6mm 

(Part No. 81404) 

Mobile Phase: 1%Ammonium Hydroxide in Methanol:Acetonitrile (25:75) 1) Doxorubucine 


2) Daunorubicine 


Antiseptics 

0 1 

Highly Polar Cosmetic Ingredients 

2 

1 

2 

3 4 5 Min. 

1. Allantoin 

2. Bronopol 

Column: ZirChrom ® CARB, 3µm, 100 x 4.6mm 

(Part No. ZR01-1046) 




10166 

Chemotherapeutics 

Rubicines - Chemotherapeutics 

1 

2 

1. Doxorubucine 

2. Daunorubicine 

0 5 10 15 20 Min. 

Column: Grom Sil 120 ODS-3 CP, 5μm, 125 x 3mm 

(Part No. 5113604) 

Mobile Phase: A: 25mM Sodium Phosphate, pH2.2 B: Acetonitrile 


%B: 0 60 60 


Detector: Fluorescence at 480nm (exc.), 560nm (em.) 

Rubicines - Chemotherapeut 

Column phase: GROM-SIL 1 

Column size: 125 x 3mm 

Eluent A: 25 mM Na-phosph 

B: ACN 

Gradient: 0-60% B (0-12 mi 

Flow rate: 0.45 ml/min 

Pressure: 19 MPa 


Detection (Fluor): 480 nm (e 

Injection: 100 µl (100 ng/ml 




403



Diuretics 

2 

1 

4 

3 

10 

7 

5 6 8 9 

12 

11 

13 

14 

15 

Diuretics and Uricosuric Agents 

1. Amiloride HCl Hydrate 

2. Acetazolamide 

3. Hydrochlorothiazide 

4. Triamterene 

5. Chlorthalidone 

6. Furosemide 

7. Hydroflumethiazide 

8. Clopamide 

9. Ethacrynic Acid 

10. Trichloromethiazide 

11. Probenecid 

12. Indapamide 

13. Bumetanide 

14. Bendroflumethiazide 

15. Spironolactone 

10246 

0 

5 10 15 20 25 30 Min. 


Mobile Phase: A: 0.1% Trifluoroacetic Acid in 0.025M Ammonium Acetate B: 0.1% Trifluoroacetic Acid in Acetonitrile 


%B: 25 65 

11 



10 

1 

2 3 

4 

5 

6 

7 

8 

9 

12 

Doping Agents 

1. Amiloride HCl Hydrate 



4. Chlorthalidone 


6. Clopamide 


8. Indapamide 


10. Bumetanide 

11. Probenecid 


10262 

0 

1 2 3 4 5 6 7 8 9 10 11 12 Min. 

7 




%B: 25 95 95 


3 

5 6 6. Trichloromethiazide 

7. Indapamide 


8 

Diuretics — High Throughput 



10265 

1 

9 

3. Triamterene 


5. Clopamide 

8. Bumetanide 


2 4 

0 

1 2 3 4 Min. 

Column: Alltech ® Alltima C18, 3µm, 53 x 7mm Rocket (Part No. 50605) 



%B: 20 95 



404 



Antibiotics 



1 

2 

3 4 

5 

1. Sulfanilamide 

2. Cefaclor 

3. Cefatrizine 

4. Thiamphenicol 

5. Cefotaxime 

8711 8764 

3 

1. Oxytetracycline 

Dihydrate 

1 

2. Tetracycline HCl 

2 

3. Meclocycline 

Sulfosalicylate Salt 


0 2 

4 6 8 10 Min. 

Column: Alltech ® Platinum EPS C18, 5µm, 150 x 4.6mm 

(Part No. 32043) 




0 2 4 6 8 10 Min. 


Mobile Phase: A: 0.05M KH2PO4, pH3.0 B: Acetonitrile 


%B: 15 40 



Aminoglycoside Antibiotics 


2 

1 

3 

4 

1. Streptomycin 

2. Amikacin 

3. Tobramycin 

4. Neomycin 

9210 

1 

2 

3 

6 

4 

5 

9 

7 8 

11 

10 

1. Neomycin 

2. Amoxicillin 

3. Vancomycin 

4. Cefatrizine 

5. Metampicillin 

6. Cefotaxime 

7. Unknown 

8. Bacitracin 

9. Pipericillin 

10. Bacampicillin 

11. Oxacillin 

9396 

0 1 

2 

3 

4 

5 6 7 8 9 Min. 

0 2 

4 6 8 10 12 14 16 18 20 22 Min. 


Mobile Phase: 0.3% Pentafluoropropionic Acid in 43.7mM Ammonium 

Formate, pH2.7:0.3% Pentafluoropropionic Acid in 

Methanol (45:55) 






%B: 10 40 40 



Tylosin and Monensin 

Sulfa Drugs 

10319 10351 

1 

1. Tylosin (400ppm) 

2. Monensin (180ppm) 

1 

1. Sulfanilamide 



4. Sulfamethizole 

2 

2 3 4 

0 1 2 3 4 5 Min. 

Column: Alltech ® Prevail Select C18, 5µm, 150 x 4.6mm 

(Part No. 99300) 

Mobile Phase: Ammonium Hydroxide in Water, pH9.0:Acetonitrile (5:95) 



0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min. 


(Part No. 87697) 

Mobile Phase: 0.1% Phosphoric Acid:Acetonitrile (85:15) 






405

mAU 



Antibiotics 

1 

2 

Antibiotics via HILIC 

0 5 10 15 20 25 30 35 40 45 Min. 

3 

1. Amikacin 

2. Tobramycin 

3. Neomycin 

10626 

1 2 3 45 6 7 

8 

Column: Alltech ® Alltima HP HILIC, 5µm, 150 x 1) 4.6mm Bacampiccillin Column: Grace ® VisionHT C18-P, 1.5µm, 50 x 2.0mm 

(Part No. 86464) 

2) Methicillin 

(Part No. 5139606) 

Mobile Phase: Acetonitrile:Methanol:Water (87:11:2) 3) Benzylpenicillin Mobile Phase: A: 0.1% Formic Acid B: Methanol 


4) Phenoxymethylpenicillin Gradient: Time: 0 1.4 


5) Phen-oxyethylpenicillin 

%B: 20 100 

6) Phenoxypropylpenicillin Flow Rate: 0.4mL/min 

7) Cloxacillin Detector: UV at 280nm 

Penicillin Antibiotics 

8) Propicillin 

9) Proicillin 

10) Dicloxacillin 

Analysis of Vancomycin 

20 

15 

10 

5 

0 

Sulfa Drugs 

1. Sulfadiazine 

2. Sulfathiazole 



5. Sulfamethoxazole 

6. Sulfaisoxazole 

7. Sulfaquinoxilone 

8. Sulfamethoxine 

0 1 2 3 4 5 Min. 

2 

1. Bacampicillin 

1. N-Dimethylvancomycin 

2. Methicillin Penicillin Antibiotics 

2 

2. Vancomycin1) N-Dimethylvancomycin 

1 3 

3. Benzylpenicillin 

10 

2) Vancomycin 

4 

4. Phenoxymethylpenicillin 

7 

5. Phen-oxyethylpenicillin 

6. Phenoxypropylpenicillin 

Column phase: GROM-SIL ODS-7 pH, 4 µm 

Column phase: GROM-SIL 100 

Column size: 150 x 4.0 mm 

5 6 7. Cloxacillin 

8 9 Column size: 250 x 4 mm 

8. Propicillin Eluent 50 mM NaH2PO4 pH 2.3 

9. Proicillin 

Eluent : 0.1% H PO / ACN = 15 


10. Dicloxacillin 

1 





Temperature: ambient 

Detection (UV): 220 nm 

0 3 6 9 12 15 18 21 24 27 30 33 Min. 

0 2 4 6 8 10 12 14 16 18 20 22 Min. Detection (UV): 210 nm 

Injection: 8 µl (~ 0.13 mg/ml of each) 

Column: Grom Sil ODS-7 pH, 4μm, 150 x 4mm 

Column: Grom Sil 100 ODS-0 AB, 5μm, 250 x 4mm 

Injection: 10 µl (10 µg/ml) 

Mobile Phase: 50mM Monosodium Phosphate, pH2.3 

Mobile Phase: 0.1% Phosphoric Acid:Acetonitrile (15:85) 





10724 

0 2 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Erythromycin 

8630 

1 

1. Erythromycin 

4 6 8 10 Min. 

Alltech ® Macrosphere 300 C4, 7µm, 250 x 4.6mm 

(Part No. 71039) 

Acetonitrile: 0.2M Ammonium Acetate in Water, 

pH4.5 (95:5) 

1.0mL/min 

ELSD 

mAU 

6000000 

5500000 

5000000 

4500000 

4000000 

3500000 

3000000 

2500000 

2000000 

1500000 

1000000 

500000 

-500000 0 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Amoxicillin (U.S.P.) 

0 1 2 3 4 5 6 7 Min. 






GraceSmart C18, 5µm, 250 x 4.6mm 

(Part No. 5138810) 

0.05N Monobasic Potassium Phosphate, pH5.0: 


1.5mL/min 

UV at 230nm 

10697 

406 



Bacitracin 

Bacitracin 

0 1 2 3 4 5 6 7 8 Min. 

0 1 

Spectinomycin 

1 

1. Spectinomycin 

2 3 4 5 6 7 8 9 Min. 

10680 


Column: 

GENESIS Jones FM15960E Genesis ® (C18, C18, 4 4µm, 4.6150 mm x i.d. 4.6mm x 150 mm) 

Flow: 

1.0 mL/min (Part No. FM15960E) 

Mobile Eluent: Phase: 31% Acetonitrile:0.1% ACN/0.1% TFA in water Trifluoroacetic Acid (31:69) 

Flow Detection: Rate: Absorbance, 1.0mL/min 214 nm 


Column: Alltech ® Alltima HP C18 Amide, 3µm, 50 x 4.6mm 

(Part No. 87829) 

Mobile Phase: 10mM Ammonium Carbonate:Acetonitrile (98:2) 



mAU 

100 

80 

60 

40 

Clarithromycin on 


1. Clarithromycin 

10760 

mAU 

500 

400 

300 

200 

Clindamycin Hydrochloride on 


1. Clindamycin 

10768 

20 

100 

0 

0 

2 4 6 8 10 12 14 16 18 Min. 








Mobile Phase: Phosphate Buffer (Dissolve 9.11g Potassium Dihydrogen 

Phosphate in 1000mL Water, add 2mL Triethylamine, adjust 

with Phosphoric Acid to pH of 5.5):Acetonitrile (60:40) 



0 

0 2 4 6 8 10 12 14 Min. 








Mobile Phase: Methanol:Ammonium Dihydrogen Phosphate Solution 

(Dissolve 2.88g Ammonium Dihydrogen Phosphate in 

1000mL Water, adjust with Phosphoric Acid to pH3.0) 

300:210 



mAU 

1400 

1200 

1000 

800 

600 

400 

200 

0 

0 

Ceftriaxone Sodium on 


1. Ceftriaxone 

5 10 15 20 25 Min. 




10776 





Mobile Phase: 0.02mol/L n-Octylamine:Acetonitrile (73:27) 



mAU 

700 

600 

500 

400 

300 

200 

100 

0 

0 

Cefradine on 


1. Ceftriaxone 

5 10 15 20 25 30 35 40 45 50 Min. 




10767 





Mobile Phase: Water:Methanol:3.86% Sodium Acetate:4% Acetic Acid 

(1564:400:30:6) 






407



Bronchodilators 



3 

4 

1 

5 

1. Uracil 

2. Salbutamol 

3. Terbutaline 


5. Pseudoephedrine 

1 

2 

3 

Analysis of Broncholytics - 

Xanthines 

4 

5 



3. β-Hydroxyethyltheophylline 

4. 7-β-Hydroxypropyltheophyline 

5. Caffeine 

2 

0 1 2 3 4 Min. 

Column: Jones Genesis ® Phenyl, 4µm, 150 x 4.6mm 

Column: GENESIS (Part FM15980E No. FM5980E) (Phenyl, 4 µm, 4.6 mm i.d. x 150 mm) 

Flow: Mobile Phase: 2.0 mL/min Acetonitrile:25mM KH2PO4, pH3.0 (10:90) 

Eluent: Flow Rate: Isocratic, 2.0mL/min 10% ACN in 25 mM KH 2 PO 4 , pH3.0 

Detection: Detector: Absorbance, UV at 220nm 

Peaks: 1. Uracil, 2. Salbutamol, 3. Terbutaline, 4. Theophylline, 


Xanthines 

2 

1 

3 

4 

5 

6 7 

0 2 4 6 8 10 Min. 

1. Uracil 


3. Impurity 

4. Sodium Benzoate 


6. b-Ethoxytheophylline 

7. Caffeine 

Xanthines 

0 3 6 9 12 Min. 

Column: Grom Sil 120 ODS-4 HE, 5μm, 250 x 4mm 

(Part No. 5113626) 

Mobile Phase: 0.4% Sodium Acetate:Acetonitrile (85:15) 



Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Jones Genesis ® C18, 4µm, 150 x 4.6mm 


Acetonitrile:50mM Aqueous Ammonium Acetate, pH4.7 

(12:88) 

1.0mL/min 

UV at 254nm 

Column: GENESIS FM15960E (C18, 4 µm, 4.6 mm i.d. x 150 mm) 

Flow: 

1.0 mL/min 

Eluent: Antifungal Isocratic, 12% ACN in 50 mM NH 4 OAc, pH 4.7 


Lotrimin ® Topical Assay 

Peaks: 1. Uracil, 2. Theobromine, 3. Impurity, 4. Sodium Benzoate, 

5. Theophylline, 6. b-Ethoxytheophylline, 7. Caffeine 

1 

2 

0 2 4 6 8 10 12 14 16 18 20 22 24 Min. 

1. PEG 

2. Clotrimazole 

10447 


Mobile Phase: A: Water to pH7.0 with Acetic Acid B: Methanol 


%B: 30 50 100 100 30 



mAU 

400 

350 

300 

250 

200 

150 

100 

50 

0 

Griseofulvin Assay on Chinese Pharmacopeia 

0 

1. Griseofulvin 

2.5 5 7.5 10 12.5 15 17.5 20 22.5 Min. 




10772 





Mobile Phase: 0.05mol/L Potassium Dihydrogen Phosphate: 

Acetonitrile:Methanol (57:38:5) 



408 



Taxols 

Taxol ® and Related Taxanes in Yew 

Taxanes 

1. 10-Deacetylbaccatin III 


3. 7-Xylosyl-10-deacetyltaxol B 

4. Taxinine M 

5. 7-Xylosyl-10-deacetyltaxol 

6. 7-Xylosyl-10-deacetyltaxol C 

1 

2 

4,5 6 

3 

7 

8 9 

10 11 

7. 10-Deacetyltaxol 

8. 7-Xylosyltaxol 

9. Cephalomannine (Taxol ® B) 

10. 7-Epi-10-deacetyltaxol 

11. Taxol ® 


13. 7-Epitaxol 

12 13 

9221 

1. 10-Deacetylbaccatin III 


3. 10-Deacetyl-7-xylosyltaxol B 

4. Taxinine M 

5. 10-Deacetyl-7-xylosyltaxol 

6. 10-Deacetyl-7-xylosyltaxol C 

7. 10-Deacetyltaxol 

8. 7-Xylosyltaxol 

1 

2 

3 4 

a 

5 6 8 10 11 

7 9 

Taxanes 

12 13 

9. Cephalomannine 

10. 10-Deacetyl-7-epitaxol 

11. Taxol ® 


13. 7-Epitaxol 

b 

Peaks: 

a. 3’-Fluorophenyl-10-deacetyltaxol 

b. 3’-Fluorophenyltaxol 


1. 10-deacetylbaccatin III 

2. baccatin III 

3. 10-deacetyl-7-xylosyltaxol 

4. taxinine M 

5. 10-deacetyl-7-xylosyltaxol, 

6. 10-deacetyl-7-xylosyltaxol 

7. 10-deacetyltaxol 

8. 7-xylosyltaxol 

9. cephalomannine 

10. 10-deacetyl-7-epitaxol, 

11. paclitaxel 

12. taxol C 

13. 7-epitaxol 

a. 3’-fluorophenyl-10-deacety 

b. 3’-fluorophenyltaxol 

0 1 

2 

3 

4 

5 6 

7 

8 Min. 0 10 20 30 40 50 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Alltech ® Alltima Phenyl 3µm, 53 x 7mm Rocket 

(Part No. 81174) 

0.05% Trifluoroacetic Acid:Methanol:Acetonitrile 

(50:19:31) 

2.5mL/min 

UV at 230nm 

Columns: VYDAC 218MS54 C18, 5 µm, 4.6 mm i.d. x 250mm 

Column: Flow: 

1.0 Vydac mL/min 

® C18, 5µm, 250 x 4.6mm (Part No. 218MS54) 

Mobile Eluent: Phase: A A: = 50 50mM NaOAc, Sodium pH Acetate, 6.8 pH6.8 B: Acetonitrile 

Gradient: B Time: = acetonitrile 0 2 32 42 42 

Gradient: Hold 34% B for 2 min, then linear to 44% B over 

%B: 34 34 44 60 100 

30 min, then linear to 60% B over 10 min, 

Flow Rate: then 1.0mL/min linear to 100% B in 10 min 

Detector: Detection: Absorbance, UV at 254nm 

nm 

Preparative Paclitaxel Purification 

Antiviral 

Aciclovir Assay on 

Chinese Pharmacopeia 

10764 

mAU 

3000 

2000 

1000 

0 

10 grams of 633N C18 slurried with 20ml of Isopropanol 

• ?Slurry used to pack a 10 mm i.d.x200mm length column 

• ?80/20 Methanol/Water used to elute the target compound 

?Fraction analyzed with a Grace Denali® C18 analytical column 

0 

Low Pressure Preparative Chromatography with Grace 

Davisil 

® 

633N C18 (10g) 10 mm i.d.x200mm Column 

20.0 

100% NeOH (v/v): 0.0% 

100% H2O (v/v): 100.0% 

80.0 

50.0 

50.0 

80.0 

20.0 

10 20 30 40 50 

100.0 

0.0 

45ml of Paclitaxel 

Fraction Collected 

60 70 80 Min. 

mAU 

450 

400 

350 

300 

250 

200 

150 

100 

50 

0 

0 

1. Aciclovir 

2 4 6 8 10 Min. 







Silica: Grace ® Davisil ® C18, 50µm (Part No. 5134095) 

Column: 10mm i.d. x 200mm Length 

Sample: 10g of powdered yew bark extract 

Mobile Phase: See Chromatogram 



Mobile Phase: Methanol:Water (10:90) 






409



mAU 

Calcium Channel Blockers 

140 

120 

100 

80 

60 

40 

20 

0 

0 

Flunarizine Hydrochloride Capsules on 


2 4 6 8 10 12 Min. 




10761 


Mobile Phase: Methanol:Phosphate Buffer (Dissolve 1.36g Potassium 

Dihydrogen Phosphate in 1000mL Water, add 4mL 

Triethylamine, adjust with Phosphoric Acid to pH3.5) 

(75:25) 



mAU 

250 

1. Flunarizine 200 

1. Nimodipine 




150 

100 

50 

0 

0 

Nimodipine Tablets on 


2 4 6 8 10 12 Min. 




10763 





Mobile Phase: Methanol:Acetonitrile:Water (35:38:27) 



mAU 

Beta Blockers 

30 

25 

20 

15 

10 

5 

0 

0 

1. Atenolol 

5 10 15 20 25 30 35 Min. 

Atenolol Assay on Chinese Pharmacopeia 




10777 





Mobile Phase: 700mL Phosphate Buffer (6.8g Potassium Dihydrogen 

Phosphate in 1000mL Water, adjust pH3.0 with 

Phosphoric Acid), add Methanol 300mL and Sodium 

1-Octanesulfonate 1.30g 



Histamine Receptor Antagonists 

Famotidine Tablets on 


mAU 

700 

600 

500 

400 

300 

200 

100 

0 

0 

2 4 6 8 10 12 14 16 18 20 Min. 




10779 


Mobile Phase: Sodium 1-Heptanesulfate solvent (2.0g Sodium 

1-Heptanesulfate in 900mL Water, adjust to pH 3.9 

with Acetic Acid):Acetonitrile:Methanol (25:6:1) 



250 

1. Famotidine 1. Naloxone 

200 




Narcotic Analgesic Antagonists 

Naloxone Hydrochloride Injection 

on Chinese Pharmacopeia Assay 

300 

mAU 

150 

100 

50 

0 

0 

2.5 5 7.5 10 12.5 15 17.5 20 22.5 25 Min. 




10778 





Mobile Phase: Sodium 1-octanesulfonate solvent (1.36g Sodium 

1-octanesulfonate, 1.0g Sodium Chloride in 580mL 

Water):Methanol:Phosphoric Acid (580:420:1) 



410 



0 

Ginseng 

1 

2 

Ginsenosides in Korean Ginseng 

3 

4 

5 

6 

7 8 10 

9 

1. Rh2 

2. Rh1 

3. Rg2 

4. Rg3 

5. Rg1 

6. Rf 

11 

7. Re 

8. Rd 

9. Rc 

10. Rb2 

11. Rb1 

20 40 60 Min. 

10142 


(Part No. 35101) 

Mobile Phase: A: Acetonitrile:Water:Isopropyl Alcohol (80:5:15) 

B: Acetonitrile:Water:Isopropyl Alcohol (67:21:12) 

Gradient: Time: 0 28 35 45 50 51 57 

%B: 25 85 80 75 90 100 25 



600000 

500000 

400000 

300000 

200000 

100000 

0 

-100000 

40 

12 

50 60 70 80 90 100 Min. 




Ginseneoside 

3 

1. Rg1 

2. Re 

3. Rb1 

10765 






Gradient: Time: 0 35 55 70 100 

%B: 19 19 29 29 40 



nutraceuticals | vitamins 

Ginkgo Biloba 

Folium Ginkgo 

Terpene Lactones High Throughput 

10742 

10288 

2 

1 

3 

4 

1. Ginkgolide C 

2. Bilobalide 

3. Ginkgolide A 

4. Ginkgolide B 

1. Bilobalide 

2. Ginkgolide J 




1 2 3 

4 5 

0 2.5 5 7.5 10 12.5 15 17.5 20 22.5 25 Min. 

0 

1 2 3 4 5 6 Min. 


Mobile Phase: Methanol:Tetrahydrofuran:Water (25:10:65) 




(Part No. 50605) 


B: 0.1% Trifluoroacetic Acid in Methanol 


%B: 10 45 



Ginkgo Biloba Flavonol Glycoside Standards 

Ginkgo Biloba Terpene Lactone Standards 

1 

4 

10239 10287 

2 

3 

1. Quercetin 

2. Kaempferol 

3. Isorhamnetin 

1 

23 

5 

1. Bilobalide 





0 

2 4 6 8 10 12 14 16 18 20 Min. 


(Part No. 32064) 

Mobile Phase: A: 0.05% Trifluoroacetic Acid in Water:Methanol (95:5) 



%B: 10 90 



0 

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min. 

Column: Alltech ® Alltima C18, 3µm, 100 x 4.6mm 

(Part No. 813812) 




%B: 10 55 






411



1 

Liquid Ginkgo Biloba Extract Prep 

3 

2 

0 5 10 15 20 25 30 35 40 45 Min. 

4 

5 

1. Bilobalide 





10315 

Column: Alltech ® Alltima C18, 5µm, 150 x 10mm (Part No. 81102) 




%B: 22 32 



Flavonoids 

1 

Polyphenolic Flavonoids 

0 2 4 6 8 10 12 14 16 18 20 22 24 26 Min. 

2 

3 

4 

5 

6 

1. Myricetin 

2. Quercetin 

3. Naringenin 

4. Hesperetin 

5. Apigenin 

6. Kaempferol 

9397 




%B: 20 40 40 



1 

2 

3 

4 

Flavonoid Mix 

1. Myricetin 

2. Quercetin 

3. Apigenin 

4. Kaempferol 

Flavonoid Mix 

9271 8887 

2 

1. Naringin 

1 

2. Neohesperidin 

3. Myricetin 

4. Quercetin 

5. Naringenin 

6. Kaempferol 

3 

5 6 

4 

0 1 2 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

3 4 5 Min. 0 5 

10 15 Min. 

Alltech ® Alltima C18 3µm, 53 x 7mm Rocket 

(Part No. 50605) 

0.1% Trifluoroacetic Acid in Water: 

0.1% Trifluoroacetic Acid in Acetonitrile (65:35) 

2.5mL/min 

ELSD 


Mobile Phase: A: 0.05M K2HPO4, pH2.5 B: Acetonitrile 


%B: 20 40 



St. John’s Wort 

Echinacea 

1 

Hypericins 

1. Pseudohypericin 

2. Hypericin 

9107 9110 

3 

1 

Phenolic Acids 

1. Protocatechuic Acid 

2. Chlorogenic Acid 

3. Caffeic Acid 

2 

2 

0 1 

2 

3 Min. 


(Part No. 50605) 

Mobile Phase: 0.2% Phosphoric Acid:Methanol (5:95) 


Detector: Vis at 585nm 

0 1 

2 Min. 


(Part No. 50605) 

Mobile Phase: 0.01M K2HPO4, pH2.6:Acetonitrile (80:20) 



412 



Chinese Pharmacopeia 

Fructus Liquidambaris 

Fructus Kochiae 

10743 

10744 

1. Liguidanbaric Acid 1. Momordin lc 

0 2 4 6 8 10 12 14 16 18 20 Min. 0 1 2 3 4 5 6 7 8 9 10 Min. 

Column: GraceSmart C18, 5µm, 250 x 4.6mm (Part No. 5138810) Column: GraceSmart C18, 5µm, 250 x 4.6mm (Part No. 5138810) 

Mobile Phase: Methanol:Water:Acetic Acid (87:13:0.1) 

Mobile Phase: Methanol:Water:Acetic Acid (85:15:0.2) 






Bulbus Fritillariae Thunbergii 

10745 

Rhizoma Anemarrhenae 

10746 

1 

1. Peimine 

2. Peininine 

1. Sarsasapogenin 

2 

0 2 4 6 8 10 12 14 Min. 


Mobile Phase: Acetonitrile:Water:Diethylamine (70:30:0.3) 



0 2 4 6 8 10 12 Min. 





Radix Astragali 

10747 

1. Astragaloside IV 

0 2 4 6 8 10 12 14 16 Min. 


(Part No. 5138810) 







413



mAU 

Puerarin 

500 

400 

300 

200 

100 

0 

0 5 10 15 20 25 30 Min. 




Puerarin 

1. Puerarin 

10773 





Mobile Phase: Methanol:0.1% Citric Acid (25:75) 



mAU 

Tanshinone II 

50 

40 

30 

20 

10 

0 

0 

5 10 15 20 25 30 35 40 45 Min. 




Tanshinone II 

1. Tanshinone II 

10769 








Green Tea 

Green Tea Extract - Analytical 

Green Tea Extract - Preparative 

10139 

10140 

3 

1. Caffeine 

2. Epicatechin 

3. Epigallocatechin Gallate 

1. Caffeine 



3 

1 

2 

1 2 

0 2 

4 6 8 10 12 14 16 18 20 Min. 

0 2 

4 6 8 10 12 14 16 18 


Mobile Phase: 0.05% Trifluoroacetic Acid:Acetonitrile (87:13) 







1 

2 

3 

4 

5 

6 

Catechins 

7 8 

8825 

1. Gallic Acid 

2. Epigallocatechin 

3. Catechin 



6. Caffeine 

7. Gallocatechin 

8. Epicatechin Gallate 

Catechins and Gallic Acid in Green Tea 

Catechins and Gallic Acid in Green Tea 

6 

1. Gallic 1. Acid Gallic Acid 

2. Gallocatechin 2. Gallocatechin 

1 



4. Catechin 

4. Catechin 



8 6. Epigallocatechin Gallate 


7. Gallocatechin Gallate 

7. Gallocatechin Gallate 



9. Catechin Gallate 

9. Catechin Gallate 

2 4 5 7 9 

3 

414 

0 1 

2 


3 

Column: Alltech ® Platinum EPS C18, 

1.5µm, 33 x 7mm Rocket (Part No. 50577) 




4 

5 Min. 0 2 4 6 8 10 12 14 16 18 20 22 Min. 


Mobile Column: Phase: DENALI A: Acetonitrile:25mM 238DE5415 (5 µm, 4.6 Phosphoric mm i.d. x 150 Acid mm) (10:90) 

Flow: 

1.0 B: mL/min Acetonitrile:25mM Phosphoric Acid (90:10) 

Eluent: A = 10:90 (v/v) ACN:25 mM phosphoric acid 


B = 90:10 (v/v) ACN:25 mM phosphoric acid 

B%: 0 0 15 

Gradient: Hold 100% A for 3 min, then 0 to 15% B over 19 min 

Flow Temp: Rate: 25 °C 1.0mL/min 

Detector: Detection: Absorbance, UV at 280nm 

nm 

Sample: 10 µL of green tea extract


Vitamins 

1 

2 3 

5 6 

4 

7 

8 

9 

0 5 10 15 20 25 30 Min. 

1 

Water- and Fat-Soluble Vitamins 

2 3 

4 

5 

6 

7 

10 

12 14 

11 13 15 

9545 

1. Thiamine 





6. Riboflavin 

7. Cyanocobalamin 

8. Biotin 

9. Vitamin A 

10. Δ-Tocopherol 



13. Vitamin D2 

14. Vitamin D3 

15. Vitamin K 


Mobile Phase: A: Water pH3.2 w/Formic Acid 

B: Acetonitrile:Methanol (83:17) 

Gradient: Time: 0 3 10 10.1 30 

%B: 0 0 45 100 100 



Fat-Soluble Vitamins and Tocopherols 

9390 

1. Vitamin A 



4. α-Tocopherol (Vitamin E) 

5. Vitamin D2 

6. Vitamin D3 

7. Vitamin K 

1 

2 

3 

4 


1 

5 

2 

6 

7 





5. Folic Acid 


7. Riboflavin 

0 2 4 6 8 10 12 14 Min. 


Mobile Phase: A: 0.1M Ammonium Dihydrogen Orthophosphate 

Buffer, pH4.5 



B%: 5 5 40 40 



Fat-Soluble Vitamins 

1. Vitamin A (100ng on column) 

2. Vitamin E (160ng on column) 

3. Vitamin K1 (130ng on column) 

3 

10733 

10279 


0 2 4 6 8 10 12 14 Min. 


Mobile Phase: Acetonitrile:Methanol (75:25) 



0 1 2 3 4 5 6 7 8 9 10 Min. 


Mobile Phase: Methanol:Chloroform (90:10) 



1 

2 

3 

4 

5 

6 


from Multi-Vitamin Tablet 

7 

1. Thiamine 


3. Unknown 


5. Pyridoxine 



9456 

1 

2 

3 

4 


5 6 

7 





5. Folic Acid 

6. Riboflavin 


8154 

0 2 4 6 8 10 12 14 Min. 





0 2 4 6 8 10 12 14 16 Min. 


(Part No. 32043) 

Mobile Phase: A: 0.1M Ammonium Phosphate, pH4.5 



B%: 5 5 40 40 






415


1) a-Tocopherol 

2) b-Tocopherol 

3) g-Tocopherol 

4) d-Tocopherol 


Vitamins 

1 

2 

3 

4 

Analysis of Tocopherols 

1. a-Tocopherol 

2. b-Tocopherol 


4. d-Tocopherol 

Analysis of Tocopherols 

Column phase: GROM-SIL 120 Amino-2 PA, 

5 µm 

Column size: 250 x 4 mm 

Vitamin E 

Eluent: hexane / ethyl acetate = 

70 / 30 

4 


2 



Detection (UV): 295 nm, 0.08 AUFS 1 

Injection: 7 µl (250 µg/ml, each) 

6 

3 

5 

7 

Vitamin E 

8 


2. a-Tocotrienol 

3. b-Tocopherol 


5. b-Tocotrienol 

6. g-Tocotrienol 

7. d-Tocotrienol 

8. d-Tocotrienol 

0 10 20 30 Min. 0 5 10 15 20 25 Min. 

Column: Grom Sil 120 Amino-2 PA, 5µm, 250 x 4mm 

Column: Jones Genesis ® Si, 3µm, 250 x 4.6mm 

(Part No. 5113466) 

Column: GENESIS FM25961E (Part No. FM25961E) 

(Si, 3 µm, 4.6 mm i.d. x 250 mm) 

Mobile Phase: Hexane:Ethyl Acetate (70:30) 

Flow: Mobile Phase: 1.5 mL/min Hexane:1,4-Dioxane (96:4) 


Eluent: Flow Rate: Isocratic, 96:4 1.5mL/min Hexane/1,4-Dioxane 


Detection: Detector: Fluorescence, Fluorescence (294 nm excitation, 294nm 326 (exc.), nm emission) 326nm (em.) 

Peaks: 1. a-tocopherol, 2. a-tocotrienol, 3. b-tocopherol, 

4. g-tocopherol, 5. b-tocotrienol, 6. g-tocotrienol, 

Vitamin E Soft Gel Tablet 

Determination of Retinol, a-Tocopherol, 

3 

Determination 7. d-tocotrienol, of Retinol, α-Tocopherol, 8. d-tocotrienoland β-Carotene in Serum 

2 

9458 

and b-Carotene in Serum 



325 nm 295 nm 450 nm 

1. All-trans-Retinol 


7 

2. Tocol 



1 

5. Lutein 

4 6. Zeaxanthin 

5 

2 

9 

7. Cryptoxanthin 

1 

3 6 8 

8. a-Carotene 

10 9. All-trans-b-Carotene 

10. cis-b-Carotene 

0 3 6 9 12 15 Min. 

0 4 8 12 16 20 24 28 32 Min. 

Column: Alltech ® Prevail C18, 5µm, 150 x 4.6mm (Part No. 99208) Column: 

Column: VYDAC Vydac 201TP54 C4, (C18, 5µm, 5 µm, 2504.6 x 4.6mm mm i.d. (Part x 250 No. mm) 201TP54) 


Mobile Flow: Phase: 1.5 mL/min A: Buffer:Methanol:n-Butanol (15:75:10) 


Eluent: A = 15:75:10 B: Buffer:Methanol:n-Butanol buffer/methanol/n-butanol(2:88:10) 


B = 2:88:10 Buffer:Aqueous buffer/methanol/n-butanol 

0.02M Ammonium Acetate, pH3.5 

Gradient: buffer Time: = aqueous 00.023 M NH18 4 OAc, 32pH 3.5 

Gradient: 100% %B: A for 3 min, 0 then 0 0 to 100 100% 100B over 15 min, 

Figure 2. Rechromatography 

then hold 100% 

of 

B 

Flow Rate: 1.5mL/minβ-isomer-enriched tocopherol 

Detection: Absorbance, programmed wavelength 

from Figure Detector: 1. Shaded area Absorbance, indicates programmed collected wavelength fractions 

Figure 1. First step separation of tocopherol β-isomer on 

Davisil LC60Å 20-45mm Column. Shaded area indicates 

Preparative Purification Peaks: of Tocopheral 

1. All-trans-retinol (vitamin A), 2. Tocol, 3. g-Tocopherol, 4. a-Tocopherol (vitamin E), 5. L 

collected fraction 

7. Cryptoxanthin, 8. a-Carotene, 9. All-trans-b-carotene, 10. Cis-b-carotene 

First Step Separation of Tocopherol b-Isomer 

Rechromatography of b-Isomer-Enriched Tocopherol 

0 20 40 60 80 100 120 140 160 Min. 

Shaded area indicates collected fraction. 

Sample is 250g tocopherol isomer mixture in 1000mL hexane. 

Sample: 250g tocopherol isomer mixture in 1000ml hexane 

Silica: 

Column: 200 x 

Davisil 

500mm Davisil ® LC60Å ® LC60A 

20-45µm, 

20-45mm 

(Part No. 5055349) 

Column: Mobile Phase: hexane/2.5% 200 x THF 500mm 

Flow Rate: 600ml/min (115cm/hr) 

Mobile Phase: Hexane:2.5% Tetrahydrofuran 


0 20 40 60 80 100 120 Min. 

Shaded area indicates collected fractions. 

Sample is pooled fraction from first step. 

Silica: Davisil ® LC60Å 20-45µm, (Part No. 5055349) 

Column: 50 x 500mm 


Sample: Pooled fraction from run showed in Figure 1 

Column: 50 x 500mm Davisil LC60Å 20-45mm 

Flow Rate: 50ml/min (155cm/hr) 

416 



1 

2 

b–Carotenes 

3 

4 5 

0 5 10 15 20 Min. 

Column: 

Eluent: 

b–Carotenes 

a-carotene 

cis-a-carotene 

1. 2. cis-a-Caroteneb-carotene 

3. b-Carotene 13-cis-b-carotene 

4. 13-cis-b-Carotene 

5. 9-cis-b-Carotene 

9-cis-b-carotene 

VYDAC 201TP54 (C4, 5 µm, 4.6 mm i.d. x 250 mm) 

Isocratic, 94:6 MeOH/chloroform 

Column: Vydac ® C4, 5µm, 250 x 4.6mm (Part No. 201TP54) 

Mobile Phase: Methanol:Chloroform (94:6) 

mAU 

100 

80 

60 

40 

20 

0 

0 

Coenzyme Q10 

1. Coenzyme Q10 

5 10 15 20 25 30 35 40 45 Min. 




10771 





Mobile Phase: Methanol:Ethanol (50:50) 




Devil’s Claw 

1 

Iridoid Compounds 

0 1 2 3 4 5 6 Min. 


(Part No. 50605) 

Mobile Phase: 0.05% Trifluoroacetic Acid:Methanol:Dioxane (75:23:2) 



2 

9191 

1. 8-para-Coumaroyl Harpagoside 

2. Harpagoside 

Saponins 

HPLC Analysis of Saikosides 

1) Saikosaponin c 

2) Saikosaponin f 

3) Saikosaponin a 

4) Saikosaponin b2 

5) Saikosaponin b1 

6) Saikosaponin d 

3 

HPLC Analysis of Saikosides 

1. Saikosaponin c 

2. Saikosaponin f 

3. Saikosaponin a 

4. Saikosaponin Column b2 phase: GROM-SIL 120 O 

1 

5. Saikosaponin Column b1 size: 250 x 4 mm 

6. Saikosaponin d 

Eluent: H2O / MeOH / ACN = 45 / 

4 


2 

6 


5 



Injection: 5 µl (~30 µg of each) 

0 4 8 12 16 20 24 28 Min. 

Column: Grom Sil 120 ODS-3 CP, 5μm, 250 x 4mm 

Mobile Phase: Water:Methanol:Acetonitrile (45:15:40) 






417


Goldenseal Root 

1 

Alkaloids 

2 

9124 

Black Cohosh 

Black Cohosh Extract 

9496 


0 1 

1. Hydrastine 

2. Berberine 

2 Min. 


(Part No. 50605) 

Mobile Phase: 0.02M KH2PO4, pH2.6:Methanol (52:48) 



1 

2 

3 

1. Cimiracemoside A 

2. Actein 

3. 27-deoxyactein 

0 10 20 30 40 50 60 Min. 


Mobile Phase: A: 1% Formic Acid B: Acetonitrile 


%B: 20 100 100 20 20 



Glucosamine 

Glucosamine 

Evening Primrose 

Triglycerides 

1 

1 

1 

UV 

ELSD 

RI 

1. Glucosamine 

10412 

10413 

10411 

1 

2 

3 

4 5 6 7 

8 

1. LGG 

2. LGL 

3. LLL 

4. OGL 

5. PGL 

6. OLL 

7. OGO + PLL 

8. OOO 

10047 

0 1 2 3 4 5 Min. 

0 10 20 30 40 Min. 


(Part No. 35102) 







%B: 30 50 



418 



Carbohydrates 

1 

Corn Syrup Oligomers 

2 

3 

4 5 6 

7 8 

1. Dextrose 

2. Maltose 







0 1 2 3 4 5 6 7 8 Min. 

Column: Alltech ® Prevail Carbohydrate ES, 

5µm, 53 x 7mm Rocket (Part No. 35104) 



%B: 32 50 



9420 

1 

2 

3 

Corn Syrup Solids 

1. Dextrose 

2. Maltose 




4 5 

6 

13 

7 

8 

101112 

9 

9354 




9. Maltononaose 

10. Maltodecaose 

11. Maltoundecaose 

12. Maltododecaose 

13. Maltotridecaose 

0 5 10 15 20 25 Min. 


(Part No. 35101) 



%B: 35 50 



food & beverage 

Mono-, Di-, Tri- Saccharides 

14 

15 

11 

2 

7 

12 

3 

9 10 13 

4 1618 

5 6 

1 8 

17 19 

20 

21 

22 

1. iso-Erythritol 

2. Fructose 

3. Sorbitol 

4. Mannitol 

5. Glucose 

6. Inositol 

7. Sucrose 

8. Maltitol 

9. Maltose 

10009 

10. Raffinose 

11. DP3 

12. DP4 

13. DP5 

14. DP6 

15. DP7 

16. DP8 

17. DP9 

18. DP10 

19. DP11 

20. DP12 

21. DP13 

22. DP14 

1 

2 

Sugars 

1. Fructose 

2. Glucose 

3. Sucrose 

3 

10277 

0 10 20 30 40 50 60 Min. 


250 x 4.6mm (Part No. 35101) 

Mobile Phase: A: Acetonitrile B: 0.04% Ammonium Hydroxide in Water 


%B: 17 27 45 55 



1 2 3 

Beer Standards 

Unmodified Mobile Phase 

4 

5 6 

7 

1. Fructose 

2. Glucose 

3. Sucrose 

4. Maltose 




0 1 2 3 4 Min. 

Column: Alltech ® Prevail Carbohydrate ES, 

5µm, 53 x 7mm Rocket (Part No. 35104) 





Beer Standards 

1 Modified Mobile Phase 

9350 2 

9360 

1. Fructose 

2. Glucose 

3 

3. Sucrose 

4. Maltose 

4 


5 6 7 



0 5 10 15 20 25 30 35 40 Min. 

Column: Alltech ® Prevail Carbohydrate ES, 250 x 4.6mm 

(Part No. 35101) 



%B: 20 35 



0 2 

4 

6 

8 10 12 14 16 18 Min. 


(Part No. 35101) 

Mobile Phase: A: Acetonitrile:Acetone (75:25) 

B: Water 


%B: 24 50 






419



Carbohydrates 

1 2 

Sugar Alcohols 

3 4 

1. iso-Erythritol 

2. Xylitol 

3. Mannitol 

4. Maltitol 

9352 

0 5 10 15 20 25 30 35 40 45 Min. 

0 2 4 6 8 10 12 Min. 

4 

5 6 4 

7 

1 2 



(Part No. 35101) 

(Part No. 35101) 






%B: 17 35 



Imported Ale 

Beverage Additives 

3 

9351 

5 

9446 

1. Fructose 


2. Glucose 

2. Saccharin 

3. Maltose 

2 

3. Benzoic Acid 


3 

4. Aspartame 


5. Vanillin 



1 

1 

Wine Analysis - 2000 Sipaska Rosé 

2 3 4 

1. Fructose 

2. Sorbitol 

3. Glucose 

4. Maltose 

9474 

0 5 10 15 20 25 30 35 40 45 50 Min. 


(Part No. 35101) 



%B: 20 35 



0 1 2 3 Min. 


Mobile Phase: Acetonitrile:0.05M Ammonium Acetate, pH5.4, (20:80) 



Wine Analysis by Ion Exclusion 

5411 

Partial Digest: Complex Polysaccharide 

from Plant Cell Wall 

0 

3 5 

2 

1 

4 

6 

7 

10 

8 9 

10 20 30 40 50 Min. 



3. Glucose 

4. Malic Acid 

5. Fructose 


7. Glycerol 


9. Methanol 

10. Ethanol 

Column: Alltech ® IOA-1000, 9mm, 300 x 7.8mm (Part No. 9646) 




Detector: RI 

Partial Digest: Complex Polysaccharide from Plant Cell Wall 

0 15 30 45 60 75 90 Min. 

Columns: VYDAC 218TP54 C18, 5 µm, 4.6 mm i.d. x 250mm 

Flow: Column: 1.0 mL/min Vydac ® C18, 5µm, 250 x 4.6mm (Part No. 218TP54) 

Gradient: Linear, 7 7 to to 10% Methanol MeOH over over 60 min., 60 min., then to 25% 

MeOH then at 90 to min 25% Methanol at 90 min 

Detection: Flow Rate: Evaporative 1.0mL/min light-scattering detection (ELSD) 


420 



3 

1 

2 4 

Conquistador ® Tequila 

0 2 4 6 8 10 12 14 16 18 Min. 

5 

1. Fructose 

2. Mannitol 

3. Glucose 

4. Sucrose 

5. Unknown 

10585 


(Part No. 35101) 




1 

2 

3 4 

Splenda ® Sweetener 

0 5 10 15 20 25 30 35 40 Min. 

1. Sucralose 

2. Glucose 

3. Maltose 


10531 


(Part No. 35101) 



%B: 20 20 30 30 50 




1 

2 

3 

4 

6 

5 

7 

Pepsi One ® Cola 

8 

1. Sucralose 

2. Unknown 1 

3. Fructose 

4. Glucose 

5. Unknown 2 

6. Sucrose 

7. Maltose 


10530 

1 

2 

Infant Formula 

1. Sucrose 

2. Lactose 

10529 

0 5 10 15 20 25 30 35 40 Min. 


(Part No. 35101) 



%B: 20 20 30 30 50 



0 5 10 15 20 25 30 35 40 Min. 


(Part No. 35101) 



%B: 20 20 30 30 50 



Flavor Sample Solution 

Chromatogram courtesy of Charles Stewart, Firmenich Inc. 

10579 

Spiked Urine 

Chromatogram courtesy of Rosemary Rose, 

Monash University, Dept. of Gastroenterology 

10584 

1 

2 

3 

1. Fructose 

2. Glucose 

3. Sucrose 

1 

2 3 

1. Sucralose 

2. Rhamnose 

3. Cellobiose 

0 5 10 15 20 25 Min. 


(Part No. 35101) 



%B: 20 29 



0 1 2 3 4 5 6 7 8 9 Min. 


(Part No. 35102) 







421


Carbohydrates 


10557 

Cough Syrup 

10556 


1 

2 

3 

4 

5 6 

0 5 10 15 20 25 30 35 40 Min. 

1. Sucralose 

2. Fructose 

3. Sorbitol 

4. Glucose 

5. Sucrose 

6. Maltose 


(Part No. 35101) 



%B: 15 15 25 25 50 



1 

2 

3 

4 

0 5 10 15 20 25 30 35 40 Min. 

1. Fructose 

2. Glucose 

3. Sucrose 

4. Maltose 


(Part No. 35101) 



%B: 20 20 30 30 50 



1 

2 

3 

Tobacco 

0 5 10 15 20 25 30 35 40 Min. 

4 

1. Fructose 

2. Glucose 

3. Sucrose 

4. Unknown 

10526 

1 

2 

3 

Kellogg's Frosted Flakes ® Cereal 

Regular vs. 1/3 less sugar 

4 5 

1 2 4 

5 

0 5 10 15 20 25 30 35 40 Min. 

3 

Regular 

1. Fructose 10527 

2. Glucose 

3. Sucrose 

4. Maltose 

5. Oligosaccharides 

1/3 Less Sugar 

10528 


(Part No. 35101) 



%B: 20 20 30 30 50 




(Part No. 35101) 



%B: 20 20 30 30 50 



Rosenta Analysis 

0 5 10 15 20 25 30 35 40 Min. 

Column: Grom Sil 100 ODS-2 FE, 5μm, 250 x 4mm 



%B: 25 100 



422 



Organic Acids 

1 

8 

2 

4 6 7 

3 5 

9 10 

11 

0 1 2 3 4 5 6 7 8 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Organic Acid Mix 




4. Malic Acid 



7. Diglycolic Acid 





Alltech ® Prevail Organic Acid, 5µm, 150 x 4.6mm 

(Part No. 88640) 

0.025M KH2PO4, pH2.5 

1.5mL/min 

UV at 210nm 

9384 

1 

2 

3 

4 

0 2 4 6 8 10 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Rye Bread Extract 

5 


2. Malic Acid 




Alltech ® Prevail Organic Acid, 5µm, 150 x 4.6mm 

(Part No. 88640) 

0.025M KH2PO4, pH2.5 

1.0mL/min 

UV at 210nm 

9484 


1 

2 

3 

4 

Underivatized Fatty Acids 

5 

6 

1. Lauric Acid 

2. Myristic Acid 

3. Palmitic Acid 

4. Stearic Acid 

5. Arachidic Acid 

6. Behenic Acid 

9539 

5 

3 

1 

2 4 6 

7 

Organic Acid Mix 

8244 


2. Malic Acid 


4. Diglycolic Acid 




0 2 4 6 8 10 12 14 16 18 20 Min. 

Column: Alltech ® Prevail Organic Acid, 5µm, 150 x 4.6mm 

(Part No. 88640) 




3 

2 

4 

6 

1 

5 

8 

7 

Short-Chain Aromatic Carboxylic Acids 

9 

10 

11 

12 

7067 



3. Shikimic Acid 



6. Homoprotocatechuic Acid 

7. Gallic Acid 

8. Protocatechuic Acid 

9. Gentisic Acid 

10. p-Hydroxybenzoic Acid 


12. Salicylic Acid 

0 10 20 30 Min. 

Column: Alltech ® OA-2000, 6.5µm, 100 x 6.5mm (Part No. 9048) 





0 2 4 6 8 10 Min. 


(Part No. 32420) 




0 

1 Fatty Acids Using UV and ELSD in Tandem 

4 


2 


3. Capric Acid 

5 


3 

5. Linolenic Acid 

6 

3 


4 7 7 

5 6 8 

8 9 10 

UV 

9 

10 

ELSD 

2 4 6 8 10 12 14 16 18 Min. 

7. Linoleic Acid 


9. Oleic Acid 


10430 


(Part No. 87686) 




%B: 80 100 100 





423


Organic Acids 

Organic Acids 

10705 

3 

4 

Long Chain Fatty Acids 

9459 


1 

2 3 

0 1 2 3 4 5 6 7 Min. 

4 

5 


2. Malic Acid 


4.Maleic Acid 


1 

2 

5 

0 1 2 3 4 Min. 

1. Capric Acid 









Iso-a-Acids in Beer 

Iso-a-Acids in Beer 

Column: Alltech ® Prevail C18, 3µm, 53 x 7mm Rocket 

(Part No. 99279) 




Oils / Lipids 

6 

Olive Oil 

9515 

1 2 

3 

4 5 6 7 

1. Iso-a-Acid 

2. Iso-a-Acid 2 

3. Iso-a-Acid 3 

4. Tetra-hydro iso-a-Acid 1 




1 2 

3 

4 

5 

7 

1. LLO 

2. LLP 

3. OOL 

4. POL 

5. PPL 

6. OOO 

7. OOP 

8. PPO 

9. OOS 

0 5 10 15 20 25 Min. 

Column: Jones Genesis ® C18, 4µm, 150 x 4.6mm + 10mm guard 

(Part No. FM15960E + FH1960-2) 

Column: GENESIS FM15960E + FH1960-2 

Mobile Phase: (C18, 4 Methanol: µm, 4.6 mm 0.1% i.d. Phosphoric x 150 mm + 10 Acid, mm 0.5mM guard) EDTA (76:24) 

Flow: Flow Rate: 1.0 mL/min 1.0mL/min 

Eluent: Detector: Isocratic, UV 76% at 260nm methanol in 

0.1% phosphoric acid, 0.5 mM EDTA 


Peaks: 1. Iso-a-acid 1, 2. Iso-a-acid 2, 3. Iso-a-acid 3, 

4. Tetra-hydro iso-a-acid Olive 1, 5. Oil Tetra-hydro iso-a-acid 2, 

6. Tetra-hydro iso-a-acid 3, 7. Tetra-hydro iso-a-acid 4 

8 9 

10561 

14 

1. LLO 

2. OOLn 

3. PLnO 

4 

4. LOO 

11 

5. PoOO 

6. PLO 

7. PoOP 

8. OOO 

9. POO+SOL 

10. POP 

5 10 

11. SOO 

6 

7 12 

1 2 12. POS+SLS 

3 

13. PPS 

13 

14. C17 

0 2 4 6 8 10 12 14 16 Min. 


(Part No. 81387) 

Mobile Phase: A: Dichloromethane B: Acetonitrile 


%B: 70 55 70 70 



Acid Whey 

3 

1. LACCER 

2. PA 

3. PE 

4. PI 

5. PS 

6. PC 

4 

5 

7. SM1 

2 

8. SM2 

6 

8 9 

1 

7 

8 

10576 

0 5 10 15 20 25 30 Min. 

Column: Alltech ® Alltima HP C18 Hi-Load, 3µm, 150 x 3.0mm 

(Part No. 87610) 



%B: 30 51 70 70 30 



0 5 10 15 20 Min. 

Column: Alltech ® Prevail Silica, 3µm, 150 x 3.0mm (Part No. 99341) 

Mobile Phase: A: Chloroform; B: Methanol; C: Triethylamine buffer pH3 


%B: 12 60 12 

%C: 0.5 12 0.5 



424 



Oils / Lipids 

2 


9517 


10581 

1 

3 

0 2 4 6 8 10 12 14 16 Min. 

1 

2 

3 

4 

5 7 

6 

8 




%B: 70 55 70 70 



4 5 6 7 

8 

1. LLL 

2. LLO 

3. LLP 

4. OOL 

5. POL 

6. PPL 

7. OOO 

8. OOP 

9. PPO 

10. OOS 

Evening Primrose Oil 

1. LGG 

2. LGL 

3. LLL 

4. OGL 

5. PGL 

6. OLL 

7. OGO + PLL 

8. OOO 

10047 

0 5 10 15 20 25 30 35 Min. 

1 

4 

1 

5 6 

2 

3 

4 

5 7 8 

9 

6 

10 

1. LLL 

2. LLO 

3. LLP 

4. OOL 

5. POL 

6. PPL 

7. OOO 

8. OOP 

9. PPO 

10. OOS 

Column: Alltima HP C18 Hi-Load, 5µm, 250 x 4.6mm 

(Part No. 87698) 



%B: 30 51 70 70 30 



Walnut Oil 

2 

1. Trilaurin (LaLaLa) 

9522 

2. Trilinolein (LLL) 

3. 1,2-Dilinoleyl-3-oleyl-rac-glycerol (LLO) 

4. 1,2-Dilinoleyl-3-palmitin-rac-glycerol (LLP) 

5. 1-Palmitin-2-olein-3-Linolein rac-glycerol (POL) 

3 

6. 1,2-Dipalmytyl-3-linolein-rac glycerol (PPL) 


9 10 0 2 4 6 8 10 12 14 16 Min. 

0 10 20 30 40 Min. 




%B: 30 50 



1 

3 

4 

5 6 7 

Corn Oil 

14 

1. C11 

2. LLLn 

3. LLL 

4. LLO 

5. PLL 

6. LOO 

7. PLO 

10566 

8. PLP 

9. OOO 

10. SLO 

11. POO 

12. POP 

13. SOO/PPP 

14 C17 




%B: 70 55 70 70 



Lecithin, 0.5mg/mL 

5 

2 

10562 

1. PA 

2. PE 

3. PI 

4. LPE 

5. PC 

6. LPC 

3 

2 

9 1011 12 

8 

13 

1 

4 

6 

0 5 10 15 20 25 30 35 Min. 


(Part No. 87610) 



%B: 30 51 70 70 30 



0 5 

Column: 

10 15 20 Min. 

Alltech ® Prevail Silica, 3µm, 150 x 3.0mm 

(Part No. 99341) 

Mobile Phase: A: Chloroform 

B: Methanol 

C: Triethylamine buffer pH3 


%B: 12 60 12 

%C: 0.5 12 0.5 






425



Oils / Lipids 

5 Palm Kernel Olein Fraction 

2 

1 

7 

3 4 

89 10 11 12 17 

14 15 16 

6 

13 

0 5 10 15 20 25 30 Min. 


150 x 3.0mm (Part No. 87610) 



%B: 30 51 70 70 30 



Coconut Oil 

2 

1 

4 5 3 

6 

7 

9 

8 10 11 12 

0 5 10 15 20 25 30 35 Min. 

1 

2 

3 

4 

5 6 7 8 9 

13 

1. CCLa 

2. C11 

3. CLaLa 

4. CCM 

5. LaLaLa 

6. CLaM 

7. LaLaM 

8. LaLaO 

9. LaMM 

1. CCLa 

2. C11 

3. CLaLa 

4. CCM 

5. LaLaLa 

6. CLaM 

10573 

10. LaMO 

11. LaMP/MMM 

12. MMO/LaPO 

13. MMP/LaPP/MPO 

14. PLP 

15. POO 

16. POP 

17. C17 

10564 

7. LaLaM 

8. LaLaO 

9. LaMM 

10. LaMO 

11. LaMP/MMM 


13. C17 


(Part No. 87610) 



%B: 30 51 70 70 30 



Sunflower Oil 

1. LLL 

2. LLO 

3. PLL 

4. LOO 

5. LLS 

6. PLO 

7. OOO 

8. SLO 

9. POO 

10567 

0 5 10 15 20 25 30 Min. 


150 x 3.0mm (Part No. 87610) 



%B: 30 51 70 70 30 



Cocoa Butter 

9 

0 5 10 15 20 25 30 35 Min. 

1 

1 

2 

3 

6 

23 4 5 7 8 

Butter 

11 

13 

10 12 

4 5 67 

8 9 10 

11 

14 

1. C13 

2. PLO 

3. PLP 

4. POO 

5. PLS 

6. POP 

7. SOO/PPP 

8. SLS 

9. POS 

10. PPS 

11. SOS 

12. PSS 

13. SOA 

14. C19 

10563 

10565 


(Part No. 87610) 



%B: 30 51 70 70 30 



Refined Sunflower Oil 

1. C11 

2. LLL 

3. LLO 

4. PLL 

5. LOO 

6. LLS 

7. PLO 

8. OOO 

9. SLO 

10. POO 

11. C17 

10569 

0 5 10 15 20 25 30 35 Min. 


150 x 3.0mm (Part No. 87610) 



%B: 30 51 70 70 30 



0 5 10 15 20 25 30 Min. 


150 x 3.0mm (Part No. 87610) 



%B: 30 51 70 70 30 



426 



Oils / Lipids 

1 

23 4 

Palm Oil 

11 

10 

9 

5 678 

12 

13 

1415 

0 5 10 15 20 25 30 35 Min. 


150 x 3.0mm (Part No. 87610) 



%B: 30 51 70 70 30 



1 

2 

3 

4 

5 

6 

7 

8 

9 

10 1112 

15 

16 

14 17 

13 18 

0 5 10 15 20 25 30 35 Min. 


(Part No. 87610) 



%B: 30 51 70 70 30 



1 

2 

19 

16 

Polar Lipid Standards 

6 

3 

4 

Soybean Oil 

5 

7 

8 

1. C13 

2. OLL 

3. PLL 

4. LOO 

5. PLO 

6. PLP 

7. MOP 

8. OOO 

1. C11 

2. LLnLn 

3. LLLn 

4. LLL 

5. OLnL 

6. PLLn 

7. LLO 

8. OOLn 

9. PLL 

10. LOO 

1. GLUCCER 

2. LACCER 

3. PE 

4. PI 

5. PS 

6. PC 

7. SM1 

8. SM2 

10571 

9. POO 

10. PLS 

11. POP 

12. SOO/PPP 

13. POS 

14. PPS 

15. SOS 

16. C19 

10570 

11. LLS 

12. PLO 

13. PLP 

14. OOO 

15. SLO 

16. POO 

17. POP 

18. SOO/PPP 

19. C17 

10575 

0 5 10 15 20 Min. 


Mobile Phase: A: Chloroform B: Methanol C: Triethylamine buffer pH3 


%B: 12 60 12 

%C: 0.5 12 0.5 



2 

1 3 4 

5 

6 

7 

8 

10 

9 11 12 

0 5 10 15 20 25 30 Min. 

Column: A Alltech ® lltima HP C18 Hi-Load, 3µm, 

150 x 3.0mm (Part No. 87610) 



%B: 30 51 70 70 30 



1 

2 

3 

4 

5 

13 

0 5 10 15 20 Min. 


Mobile Phase: A: Chloroform B: Methanol; C: Triethylamine buffer pH3 


%B: 12 60 12 

%C: 0.5 12 0.5 



1 

Palm Kernel Stearin Fraction 

2 

3 

4 

5 

6 

Butter 

6 

7 8 

9 10 

1. CCLa 

2. C11 

3. CLaLa 

4. CMM 

5. LaLaLa 

6. LaLaM 

7. LaLaO 

Gouda Cheese 

10572 

8. LaMM 

9. LaMO 

10. LaMP/MMM 

11. MMO/LaPO 


13. C17 

1. PE 

2. PI 

3. PS 

4. PC 

5. SM1 

6. SM2 

1. LACCER 

2. U 

3. PA 

4. PE 

5. PI 

6. PS 

7. PC 

8. SM1 

9. SM2 

10. LPC 

10578 

10577 

0 5 10 15 20 Min. 


Mobile Phase: A: Chloroform B: Methanol C: Triethylamine buffer pH3 


%B: 12 60 12 

%C: 0.5 12 0.5 







427


1) Limonene 

1) p-Hydroxybenzoic acid 

2) Methylhydroxybenzoic acid 

3) Propylhydroxybenzoic acid 


Oils / Lipids 

Limonene in Grapefruit Oil 

Column phase: GROM-SIL 80 ODS-2 FE, 5 µm 


Eluent A: H2O / MeOH = 50 / 50 

B: ACN 

Gradient: 0 - 100% B (0-20 min) 



Temperature: RT 

1 

Detection (UV): 254 nm, 1.0 AUFS 

Injection: 2 µl oil 

1) Sorbic acid 

0 5 10 15 20 25 Min. 2) Benzoic acid 0 1 2 3 4 Min. 

Column: Grom Sil 80 ODS-2 FE, 5μm, 2503) x Saccharin 4mm 

Column: 

Mobile Phase: A: Water:Methanol (50:50) B: Acetonitrile 


%B: 0 100 



Sweeteners in beverages Detector: UV at 254nm 


1 

2 

0 3 6 9 12 15 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Aflatoxins 

3 

Column phase: 

GROM-SIL 100 ODS-2 FE, 5 µm 

Column size: 

1. Sorbic 125 Acid x 3 mm 

2. Benzoic Eluent: Acid 

12 mM TBAH, 0.2 M Na-phosphate, 

pH 3.6 / H20 / ACN = 32 / 60 / 8 

Flow rate: 

0.4 ml/min 

Pressure: 

12 MPa 

Temperature: 

RT 

Detection (UV): 

230 nm 

Injection: 

Grom Sil 100 ODS-2 FE, 5μm, 125 x 3mm 

20 µl (50 µg/ml) 

12mM Tetrabutylammonium Hydroxide, 0.2M Sodium 

Phosphate, pH3.6:Water:Acetonitrile (32:60:8) 

0.4mL/min 

UV at 230nm 

1 

2 

3 

4 

1. Limonene 

Sweeteners in Beverages 

Aflatoxins 

3. Saccharin 

1. Aflatoxin B1 

2. Aflatoxin G1 

3. Aflatoxin B2 

4. Aflatoxin G2 

Additives 

1 2 3 

Limonene in Grapefruit Oil 

10437 

Analysis of Food Preservatives 

Column phase: 

GROM-SIL 120 ODS-3 CP, 5 µm 

1. p-Hydroxybenzoic Column size: Acid 

2. Methylhydroxybenzoic 125 x 4 mm Acid 

3. Propylhydroxybenzoic Eluent: 

Acid 

H20 / ACN = 50 / 50 (v/v) 

Flow rate: 

1.5 ml/min 

Pressure: 

15 MPa 

Temperature: 

RT 

Detection (UV): 

254 nm, 0.1 AUFS 

Injection: 

10 µl (~ 4 µg each) 1) Benzoic acid 

Grom Sil 120 ODS-3 CP, 5μm, 125 

2) 

x 

Caffeine 

4mm 

(Part No. 5115479) 

3) Aspartame 


1 

2 

0 5 10 15 Min. 

3 

Analysis of Food Preservatives 

Aspartame in Soft Drinks 


2. Caffeine 

3. Aspartame 

Analysis of Aspartame in Soft Drinks 

Column phase: GROM-SIL 120 Phenyl-1 

5 µm 


Eluent: 50 mM KH2PO4 / ACN / 

MeOH = 84 / 3 / 13 




Detection (UV): 214 nm, 0.64 AUFS 

Injection: 10 µl 

1) G2a 

2) B2a 

3) G2 

4) B2 

3 4 

Column: Grom Sil 120 Phenyl-1 FE, 5μm, 150 x 4mm 

Mobile Phase: 50mM KH2PO4:Acetonitrile:Methanol Analysis (84:3:13) of Aflatoxins by HPLC 

1 2 



Aflatoxins 

1. G2a 

2. B2a 

3. G2 

4. B2 

Column phase: 

GROM-SIL 100 ODS-0 AB, 5 µm 

Column size: 

125 x 4mm 

Eluent: 

acetic acid / MeOH / H2O = 2 / 2 / 8 

Flow rate: 

0.6 ml/min 

Pressure: 

7 MPa 

Temperature: 

RT 

Detection (Fluor.): 

360 nm (exc.), 425 nm (em.) 

Injection: 10 µl (100 µg/ml) 

0 5 10 15 Min. 

Column: Alltech ® Alltima HP Silica, 5µm, 150 x 4.6mm 

(Part No. 87795) 

Mobile Phase: Methylene Chloride:Methanol:Water (99.35:0.5:0.15) 



0 2 4 6 Min. 

Column: Grom Sil 100 ODS-0 AB, 5μm, 125 x 4mm 

Mobile Phase: Acetic Acid:Methanol:Water (2:2:8) 


Detector: Fluoresence at 360nm (exc.), 425nm (em.) 

428 



Proteins & Peptides/Reversed-Phase 

1 

4 5 8 

7 

2 3 

6 

PTH Amino Acids 

PTH Amino Acids 

9 

10 

0 2 4 6 8 10 12 14 Min. 

11 

12 

13 

1416 

17 

15 

1. His 

2. Cys Acid 

3. Arg 

4. Asp 

5. Ser 

6. Gln 

7. Thr 

8. Gly 

9. Cys 

10. Ala 

11. Tyr 

12. Met 

13. Pro 

14. Trp 

15. Phe 

16. Ile 

17. Leu 


Mobile Phase: A: Acetonitrile:25mM Potassium Phosphate, pH3.3 (20:80) 

B: Acetonitrile:25mM Potassium Phosphate, pH3.3 (80:20) 



life science 

Intensity (cps) 

Column: DENALI 238DE5415 (5 µm, 4.6 mm i.d. x 150 mm) 

Flow: 

1.0 mL/min 

Eluent: A = 20:80 ACN:25 mM potassium phosphate, pH 3.3 

B = 80:20 ACN:25 mM potassium phosphate, pH 3.3 

0 to 75% B over T17 15 min 

Ambient (23 °C) 26.4 

Absorbance, 254 nm T18-20 

31.5 T26 

5 µL of PTH amino acid standard T13 39.4 mixture 

32.8 

T5 

1) His, 2) Cys acid, 3) Arg, 4) Asp, 5) Ser, 6) Gln, 54.4 

7) Thr, 8) Gly, 9) Cys, 10) Ala, 11) Tyr, 12) Met, 

T8 

13) Pro, 17.414) Trp, 15) Phe, 16) Ile, 17) Leu 

29.6 

33.2 

7.4 

T20 T11 

19.3 25.6 

33.7 

6.0e4 Gradient: 

5.5e4 Temp: 

5.0e4 Detection: 

4.5e4 Sample: 

4.0e4 Peaks: 

3.5e4 

3.0e4 

2.5e4 

2.0e4 

1.5e4 

1.0e4 

5000.0 

T7 

7.7 16.6 

TIC of Tryptic Digest of Green Fluorescent Protein (GFP): 150pmol Load on 1mm i.d. 

22.8 

38.7 

40.1 

5 10 15 20 25 30 35 40 45 50 55 60 65 70 Min. 

T9 

55.8 

57.2 T29 

60.2 64.7 

Column: 

Vydac ® , 250 x 1mm (Part No. 238EV51) 

Mobile Phase: A: Acetonitrile:0.05% Trifluoroacetic Acid (2:98) 

B: Acetonitrile:0.05% Trifluoroacetic Acid (90:10) 


%B: 5 65 

Flow Rate: 50μL/min 

Detector: ms: ABI QSTAR 

Injection Volume: 25μL GFP digest 

Mass Spectrum of Tryptic Digest of GFP 

Intensity (counts) 

6.5 

6.0 

5.5 

5.0 

4.5 

4.0 

3.5 

3.0 

2.5 

2.0 

1.5 

1.0 

T10 

790.361 

T5 

T26 

1220.139 

1492.071 

T8 

826.410 

T18-20 

1012.464 

T24 

507.293 

T17 

T9 

T13 

T11 

1050.523 

1208.645 

804.370 

T7 

655.384 

T20 

474.329 982.501 

827.416 

T19 

523.221 1041.482 T4 

579.315 1119.553 

847.384 

1312.674 

429.090 

752.342 1511.078 

0.5 

0.0 

400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 

m/z 




429


life science 


Protein Mix 

1 

2 

3 

4 

5 

6 

1. Ribonuclease A 

2. Cytochrome C 

3. Lysozyme Impurity 

4. Lysozyme 

5. Myoglobin Impurity 

6. Myoglobin 

0 5 10 15 Min. 

Column: Alltech ® ProSphere C4, 300Å, 5µm, 150 x 4.6mm 

(Part No. 33989) 

Mobile Phase: A: 0.15% Trifluoroacetic Acid 

B: 0.13% Trifluoroacetic Acid in Acetonitrile:Water (95:5) 


%B: 30 60 



1 

Enzymes 

2 

8503 9068 

1. α-Chymotrypsinogen 

2. Carbonic Anhydrase 

0 2 4 6 8 10 12 14 Min. 


(Part No. 33989) 




%B: 40 55 



Insulins 

Bradykinins 

1 

3 

1. Bovine Insulin 

2. Bovine Insulin Impurity 

3. Porcine Insulin 

4. Porcine Insulin Impurity 

9082 

1 

2 

1. des-Pro 2 -bradykinin 

2. Bradykinin 

9062 

2 

4 

0 2 

4 6 8 10 12 Min. 0 


(Part No. 32969) 




%B: 28 38 



2 4 6 8 10 12 14 Min. 


(Part No. 32969) 




%B: 15 25 



430 




Bioactive Peptides 

1 

2 

3 

4 

5 


2. Bradykinin 

3. Leucine Enkephalin 

4. Physalaemin 

5. Substance P 

2 

Peptides 

8502 

3 4 9066 

5 

1. GLY-TYR 

1 

2. VAL-TYR-VAL 




0 2 4 6 8 10 12 14 Min. 


(Part No. 32969) 




%B: 20 50 



0 2 4 6 8 10 12 14 Min. 


(Part No. 32969) 




%B: 10 30 



life science 

2 

4 

3 

Peptide Mix 

1. Oxytocin 



4. Eledosin 

10199 

β-Lactoglobulin A Tryptic Digest 

9092 

1 

0 2 4 6 8 10 12 14 16 Min. 0 5 10 15 20 25 30 35 40 45 Min. 

Column: Alltech ® ProSphere C18, 100Å, 5µm, 250 x 4.6mm Column: Alltech ® ProSphere C18, 300Å, 5µm, 150 x 4.6mm 

(Part No. 35087) 

(Part No. 32969) 







%B: 20 50 

%B: 5 30 





Monoclonal Antibody Tryptic Digest 

10213 

0 20 40 60 80 100 120 140 160 Min. 

Column: Alltech ® ProSphere C18 100Å, 5µm, 250 x 4.6mm 

(Part No. 35087) 




%B: 0 45 






431


life science 


Peptide Mix on 300Å 

1 

2 3 

4 




1 



5 6. Substance P 

6 

0 2 4 6 8 10 12 14 16 18 20 Min. 

10363 


(Part No. 32969) 




%B: 20 40 



Phosphopeptides and Fibrinopeptide 

2 

3 

0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 Min. 

4 

1. Monophosphopeptide 

(FQpSEEQQTEDELQDK) 

2. [Glu 1 ]-Fibrinopeptide B 

(EGVNDNEEGFFSAR) 

3. Tetraphosphopeptide 

(RELEELNVPGEIVEpSLpSpSpSEESITR) 

4. Contaminant(s) 

Column: Vydac ® ProZap C18, 2.1 x 10mm 

HPLC System: HPG binary pump 



Gradient: Time: 0.0 1.0 1.1 1.3 1.4 

%B: 12 60 80 80 12 



1 


5 

6 

3 

2 

4 







10369 

BSA Tryptic Digest 

10582 

0 2 4 6 8 10 12 14 16 Min. 

Column: Alltech ® Alltima HP C18, 190Å, 5µm, 250 x 4.6mm 

(Part No. 87679) 




%B: 20 40 



0 5 10 15 20 25 30 35 40 45 50 55 Min. 

Column: Vydac ® 218MS C18, 5µm, 150 x 0.075mm 

(Part No. 218MS5.07515) 

Mobile Phase: A: Water with 0.1% Formic Acid:Acetonitrile (95:5) 

B: Water with 0.1% Formic Acid:Acetonitrile (20:80) 


%B: 0 0 50 100 

Flow Rate: 0.25µL/min 


1 3 

2 

4 

5 

6 

10370 







11111 

234 

Amino Acids by HILIC 

61 

51 

17 

10582 


2. Leucine 

3. Serine 

4. Glycine 

6. Lysine 

7. Arginine 

0 2 4 6 8 10 12 14 16 Min. 


(Part No. 35091) 




%B: 20 40 



0 1 2 3 4 5 6 7 8 9 10 11 Min. 

Column: Alltech ® Alltima HP HILIC, 150 x 4.6mm 

Mobile Phase: Acetonitrile:10mM Ammonium Acetate with 

0.2% Acetic Acid (70:30) 



432 




LC-MS of Proteins 

3 

2 

Column: Vydac ® ProZap C18, 2.1 x 10mm 

Mobile Phase: A: 0.2% Formic Acid, 0.01% Trifluoroacetic Acid in Acetonitrile:Water (5:95) 

B: 0.2% Formic Acid, 0.01% Trifluoroacetic Acid in Acetonitrile:Water (80:20) 

2 


4 

%B: 20 90 90 20 


Detector: AB/MDS Sciex Q TRAP ® MS. EMS scan 400-1700 1 amu; 

scan rate = 1000 amu/s; ion spray voltage = 5000 V; LIT fill time = 50 ms; 

1 

Q3 entry barrier = 8V; declustering potential = 20; collision energy 

3 

= 10 eV; 

curtain gas = 50; ion source gas 1 = 45; ion source gas 2 = 70; 

temperature (TEM) = 350°C. 

Left Chromatogram Injection Amount: 50 ng each 

0 1 2 3 4 5 Min. Right Chromatogram Injection Amount: 8µg each, 0 except 1 1.5µg 2for growth 3 hormone 4 5 Min. 

life science 

1. Bovine Cytochrome C, M =12222 (ave.) 

2. Horse Apomyoglobin, M = 16941 (ave.) 

3. Bovine Carbonic Anhydrase II, M = 28998 (ave.) 

4. Chicken Ovalbumin, M = 44455 (ave.) 

Peak 1 - Peak Bovine 1 - Cytochrome Bovine Cytochrome C C 

Peak 2 - Peak Horse 2 Apomyoglobin 

- Horse Apomyoglobin 

941.0 941.0 

+19+18+17+16 +15 +19+18+17+16 +14 +13 +15 +12 +14 +11 +13 +12 +10 +11 +9 +10 +9 

892.6 892.6 

+27+26+25+24+23+22+21+20 848.1 +27+26+25+24+23+22+21+20 +19 +18 848.1+17 +16 +19 +18 +15 +17 +14 +16 +13 +15 +14 +12 +13 +11 +12 +11 

942.2 942.2 

2.4 

2.0 

2.4 

2.0 

815.9 

765.1 

815.9 

874.0 

765.1 

874.0 

5 

4 

5 

4 

808.0 

808.0 

997.2 

997.2 

1.6 1.6 

1019.2 1019.2 

720.2 720.2 

1.2 1.2 

1112.1 1112.1 

0.8 0.8 

447.0 447.0 1223.0 1223.0 

680.4 680.4 1358.4 1358.4 

483.0 483.0 

0.4 0.4 644.7 644.7 

417.0 541.9 417.0 719.0 541.9 719.0 945.8 945.8 

674.0 740.5 674.0 856.7 740.5 996.2 856.7 1120.2 996.2 1120.2 

1528.3 1528.3 

569.8 707.569.8 707.5 888.6 888.6 1232.4 1232.4 

0 0 

400 400 600 600 800 800 1000 1000 1200 1200 1400 1400 1600 1600 

m/z m/z 

1059.7 1059.7 

3 3 

771.3 771.3 

2 2 

737.9 737.9 

1130.3 1304.0 1130.3 1304.0 

1210.7 1210.7 

1 1 

1412.6 1412.6 

447.0 447.0 707.0 707.0 

895.21132.8 

1132.8 1322.6 1322.6 

679.1 679.1 895.2 

425.0 425.0 740.1 805.0 740.1 805.0 1000.9 1000.9 

1216.9 1541.0 1541.0 

519.0 519.0 1216.9 

541.0 715.6 541.0 715.6 960.7 1090.7 960.7 1090.7 1694.9 1694.9 

1011.8 1011.8 1245.9 1411.61245.9 1411.6 

1187.8 1187.8 1578.6 1578.6 

400 400 600 600 800 800 1000 1000 1200 1200 1400 1400 1600 1600 

m/z m/z 

2.0 

1.8 

1.6 

1.4 

1.2 

1.0 

0.8 

447.0 

2.0 

1.8 

1.6 

1.4 

1.2 

1.0 

0.8 

425.1 

425.1 

0.6 0.6 

0.4 

0.2 

Peak 3 - Peak Bovine 3 - Carbonic Bovine Carbonic Anhydrase Anhydrase II II 

807.2 

907.1 

907.1 

+52 +51 +50 +49 +48 +47 +46 +45 +44 +43 +42 +41 +40 +39+38+37+36+35+34+33+32+31+30+29+28 +52 +51 +50 +49 +48 +47 +46 +45 +44 +43 +42 +41 +40 +39+38+37+36+35+34+33+32+31+30+29+28 +27 +26 +25 +24 +27 +23 +26 +22 +25 +21 +24 +23 +20 +22 +19 +21 +20 +19 

879.2 

879.2 

852.9 

852.9 

935.4 

935.4 

881.1 1037.1 881.1 1037.1 

966.6 

966.6 

1000.0 

1000.0 

447.0 

828.5 

807.2 968.5 968.5 828.5 

1116.4 

1116.4 

764.1 

764.1 

1161.2 

1161.2 

893.4 

893.4 

1266.8 

1266.8 

744.1 784.9 744.1 784.9 

1319.3 

1319.3 

416.9 416.9 765.9 871.5 948.1 765.9 871.5 948.1 1209.4 

1209.4 

1038.3 

1038.3 

505.0 

505.0 

1451.1 

1451.1 

482.90.4 

482.9 726.1 799.2 912.0726.1955.7 

799.2 912.01060.1 955.7 1160.3 1060.1 1160.3 

978.6 

978.6 

1381.9 

1381.9 

467.1 593.2 467.1 689.3 593.2 689.3 

691.8 691.8 930.7 1052.5 930.7 1131.7 1323.4 1052.5 1131.7 1323.41527.6 

1527.6 

525.90.2 

525.9 

992.3 992.3 1353.0 

1353.0 

418.7 590.8418.7 

630.3 590.8 630.3 776.6 776.6 1452.4 1612.2 1452.4 1612.2 

1231.8 1330.0 1231.8 1330.01477.2 

1477.2 

1363.2 

1363.2 

400 400 600 600 800 800 1000 1000 1200 1200 1400 1400 1600 1600 

m/z m/z 

4 

3 

2 

1 

0 

615.9 

4 

3 

2 

621.0 678.8 729.9621.0 

678.8 882.8 1184.7 1471.9 

1184.7 1471.9 

729.9 1643.9 

882.8 1643.9 

1313.2 

1313.2 

1355.0 1355.0 1464.9 1527.0 1464.9 1527.0 

736.9 635.8 774.1 1248.2 

736.9 895.8 774.1 1248.2 1480.2 

895.8 1480.2 

635.8 1655.9 

1655.9 

1 

Peak 4 - Peak Chicken 4 - Chicken Ovalbumin Ovalbumin 

940.7 

940.7 

+35 +34 +33 +32 +35 +31 +34 +30 +33 +29 

1012.2 

+32 +31 +30 +29 

1012.2 

961.8 

961.8 

997.7 1048.0 997.71163.3 

1048.0 1163.3 

1054.7 1077.2 1140.3 1054.7 1077.2 1140.3 1348.3 

1348.3 

955.4 

955.4 1235.6 1235.6 1482.8 1521.6 1482.8 1521.6 

615.9 919.3 919.3 1005.1 1131.4 1005.1 1131.4 1387.9 

1387.9 

967.5 967.5 1229.1 

1229.1 1434.1 

1434.1 1639.3 

1639.3 

908.3 1088.1 908.3 1117.8 1088.1 1117.8 1257.0 1396.0 1257.0 1396.0 

872.4 

945.8 872.4 

945.8 1200.3 1200.3 1592.7 

1592.7 

897.6 897.6 1114.9 1114.9 1282.0 1351.1 1282.0 1351.1 1515.4 

1515.4 

700 

0 

700 900 9001100 11001300 13001500 15001700 

1700 

m/z m/z 

1520.3 1637.8 1520.3 1637.8 

669.8 753.0 669.8 

694.5 1365.2 1502.3 

843.5 694.5 1365.2 1502.31610.7 

753.0 843.5 1610.7 

1569.6 

1569.6 

1664.3 

1664.3 




433



LC-MS of Proteins 

life science 

1 

2 

0 1 2 3 4 5 Min. 

1. Human Insulin, M = 5803 (ave.) 

2. Human Serum Albumin, M = 66520 (ave.) 

3. Human Growth Hormone, M = 22123 (ave.) 

3 

Column: Vydac. ® ProZap C18, 2.1 x 10mm 

Mobile Phase: A: 0.2% Formic Acid, 0.01% Trifluoroacetic Acid in Acetonitrile:Water (5:95) 

B: 0.2% Formic Acid, 0.01% Trifluoroacetic Acid in Acetonitrile:Water (80:20) 


%B: 20 90 90 20 


Detector: AB/MDS Sciex Q TRAP ® MS. EMS scan 400-1700 amu; 

scan rate = 1000 amu/s; ion spray voltage = 5000 V; LIT fill time = 50 ms; 

Q3 entry barrier = 8V; declustering potential = 20; collision energy = 10 eV; 

curtain gas = 50; ion source gas 1 = 45; ion source gas 2 = 70; 

temperature (TEM) = 350°C. 

Left Chromatogram Injection Amount: 50 ng each 

Right Chromatogram Injection Amount: 8µg each, except 1.5µg for growth hormone 


1.0 

0.8 

0.6 

0.4 

0.2 


1.0 

0.8 

0.6 

0.4 

0.2 

Peak 1 -Human Peak 1 -Human Insulin Insulin 

Peak 2 Peak - Human 2 - Human Serum Serum Albumin Albumin 

1161.4 

1451.6 

+7 1161.4 +6 +5 1451.6 

+7 +6 +5 

968.1 

968.1 

1453.1 

1474.5 

827.6 

1453.1 

1262.3 

1158.0 1180.4 

446.0 651.8 969.8 1040.1 

608.8 1474.5 

827.6 755.6 1465.1 

943.6 1340.1 1500.6 

817.9 1685.4 

1158.0 1180.4 

446.0 608.8 651.8 

400 755.6600 817.9 943.6 969.8 

800 

1040.1 

10001262.3 

1340.1 12001465.1 

1500.6 1400 1685.4 1600 

400 600 800 1000 1200 1400 1600 

m/z 

m/z 


2.0 

2.0 

1.6 

1.2 

0.8 

0.4 

1473.4 

+59+58 +57 +56 +55 +54 +53 +52 +51 +50 +49 1473.4 +48 +47 +46 +45 +44 +43 +42 +41 

+56 +55 +54 +53 +52 +51 +50 +49 +48 +47 +46 +45 +44 +43 +42 +41 

+59+58 +57 

1542.8 

1542.8 

1478.8 

1387.2 

1387.2 

1478.8 

1408.8 

1408.8 1446.9 

1.6 

1446.9 1620.6 

1331.9 

1415.8 

1620.6 

1513.0 

1331.9 1415.8 1360.0 

1444.9 1513.0 

1360.0 1305.0 

1.2 

1305.0 

1444.9 1356.5 

1547.8 

1281.2 1356.5 

1393.9 1547.8 

1389.7 

1475.7 

1281.2 1393.9 

1509.8 

1389.7 1329.2 1420.9 

1255.1 

1621.8 

1420.9 1399.6 

1475.7 1509.8 

1255.1 

1452.2 

1329.2 

1335.1 

1621.8 

0.8 

1282.7 1399.6 

1552.8 

1256.1 

1492.8 1585.0 

1335.1 

1452.2 

1403.5 1464.8 

1282.7 1552.8 

1256.1 

1363.5 1492.8 1585.0 

1529.4 

1323.6 1403.5 1464.8 

1582.6 1646.6 

1213.7 

1423.9 

1240.21363.5 1529.4 

1489.3 

1317.5 1537.4 

1582.6 1323.6 1646.6 

1213.7 

1266.7 

1423.9 

1240.2 1189.2 1489.3 1505.8 

1265.1 1599.8 1657.5 

1317.5 

0.4 

1189.2 1168.5 1266.7 1265.1 1270.7 

1537.4 

1319.3 

1376.3 1576.4 

1505.8 

1599.8 1657.5 

1220.2 

1642.9 1694.3 

1130.4 

1319.3 1376.3 1576.4 

1150.7 1168.5 1143.7 1220.2 

1642.9 

1567.8 

1087.5 

1270.7 1694.3 

1016.5 1678.7 

1130.4 1150.7 1567.8 

1087.5 1143.7 

1016.5 1678.7 


1100 1200 1300 1400 1500 1600 1700 

1100 1200 1300 1400 1500 1600 1700 

m/z 

m/z 


2.0 

1.8 

1.6 


1.4 

1.2 

1.0 

0.8 

0.6 

0.4 

0.2 

2.0 

1.8 

1.6 

1.4 

1.2 

1.0 

0.8 

0.6 

0.4 

447.0 

447.0 483 

418.9 

483.0 

418.9 

0.2 504.9 524 

524.9 6 

400 

400 

Peak 3 - Human Growth Hormone 

2.0 

1474.5 

+26+25+24 +23 +22 +21 +20 +19 +18 +17 +16 +15 

+43 +42 +41 

.8 

1620.6 

7.8 

1621.8 

2.8 1585.0 

1582.6 1646.6 

1599.8 1657.5 

6.4 

1642.9 1694.3 

1567.8 

1678.7 

1600 1700 


1.8 

1.6 

1.4 

1.2 

1.0 

0.8 

0.6 

0.4 

0.2 

1054.4 

1107.4 

1165.4 

1164.6 

1229.1 

1302.1 

1382.5 

1579.7 

483.0 

447.0 

1006.3 

1231.3 

1108.6 1388.7 

962.6 

418.9 504.9 664.2 

1307.3 

964.8 1136.3 

1201.1 

922.4 

714.9 

1374.0 

524.9 1587.4 

885.6 

602.9 629.7 1493.8 

1248.4 1396.8 

989.5 1127.3 1604.4 

1032.9 

822.3 1528.7 

400 600 800 1000 1200 1400 1600 

m/z 

434 




Human Growth Hormone (hGH) Assay on 300Å C4 Columns 

2 

2 

1 

1 

1 

1 2 

0 20 40 60Min. 

1. Desamido hGH 

2. hGH 

2 

VYDAC ® MS C4 Column 




Columns: Vydac ® MS C4 (Part No. 214MS54), Competitor 1 C4, Competitor 2 C4, and Competitor 3 C3 

(all 300Å, 250 x 4.6mm packed with 5µm except 4µm for Competitor 1) 

Mobile Phase: 50mM Tris, pH7.5:n-Propanol (71:29) 



life science 

1 

2 4 

4 

1 

4 

3 

2 

4 

1 3 

4 

2 

1 

3 

2 

1 2 

10 20 30 40 50 60 65 Min. 

Transmembrane Protein p14 on 300Å C4 Columns 

VYDAC ® MS C4 Column 








4. Triton X (Surfactant) 

Columns: Vydac ® MS C4 (Part No. 214MS54), Competitor 1 C4, Competitor 2 C3, Competitor 3 C4, and Competitor 4 C4 

(all 300Å, 250 x 4.6mm packed with 5µm except 4µm for Competitor 1) 

Mobile Phase: A: 0.1% Trifluoroacetic Acid in Water B: 0.085% Trifluoroacetic Acid in Acetonitrile 

Gradient Vydac ® , Comp. 1, & Comp. 2: Time: 0 20 25 45 

%B: 20 60 80 80 

Gradient Comp. 3 & Comp. 4: Time: 0 20 25 45 50 80 

%B: 20 60 80 80 90 90 



Tryptic Digest of Fetuin on 300Å C18 Columns 

VYDAC ® MS C18 

Column 


Column 


Column 

0 20 40 60 80 Min. 

Columns: Vydac ® MS C18 (Part No. 238MS5415), Competitor 1 C18, and Competitor 2 C18 (all 300Å, 5µm, 150 x 4.6mm) 

Mobile Phase: A: 0.1% Trifluoroacetic Acid in Water B: 0.085% Trifluoroacetic Acid in Acetonitrile 


%B: 4 4 40 90 90 






435


life science 



7 

6 

5 

4 

3 

2 

1 

Base Peak Chromatogram of 

Tryptic Digest of BSA 

0 

0 5 15 25 35 45 55 65 75 85 95 Min. 

Column: Vydac ® Everest ® C18, 300Å, 5µm, 300µm x 150mm 

(Part No. 238EV5.315) 

Mobile Phase: A: 0.1% Formic Acid in Water 

B: 0.1% Formic Acid in Acetonitrile 


%B: 4 4 40 90 90 

Flow Rate: 5µL/min 

Column Temp: 22ºC 

Peptide Load: 0.1µL injection of 5.8µg/µL (580ng or 9pmol) 

MS Detector: AB/MDS Sciex Q-Trap. 


Standard 


7 

4 6 5 

1 2 3 

0 5 10 15 20 25 30 35 Min. 

8 

11 

10 


Column: Mobile Phase: VYDAC A: 218TP54 Acetonitrile:0.1% (C18, 5 µm, Trifluoroacetic 4.6 mm i.d. x 250 Acid mm) (25:75) 

Flow: 

1.2 mL/min B: Acetonitrile:0.1% Trifluoroacetic Acid (58:42) 

Temp: Gradient: 50 °CTime: 0 33 

Eluent: A = 25% %B: ACN/0.1% 0 100 TFA in water 

Flow Rate: B = 58% 1.2mL/min ACN/0.1% TFA in water 

Gradient: 0-100% B in 33 min 

Detector: UV 

Peaks: 1. apoC-IIIa; 2. apoC-IIIb; 3. apoC-IIa; 4. apoC-IIIc; 

5. apoC-I; 6. apoC-IIb; 7. apoA-Ia; 8. apoA-Ib; 

9. apoA-IIc; 10. apoA-IIa; and 11. apoA-IIb. 

The standard was human insulin. 

9 

1. ApoC-IIIa 

2. ApoC-IIIb 

3. ApoC-IIa 

4. ApoC-IIIc 

5. ApoC-I 

6. ApoC-IIb 

7. ApoA-Ia 

8. ApoA-Ib 

9. ApoA-IIc 

10. ApoA-IIa 

11. ApoA-IIb 


1 


2 

6 

4 5 

3 

7 

8 

1. chicken 

2. bovine 

1. 3. ovine Chicken 

2. 4. rabbit Bovine 

3. 5. human Ovine 

4. 6. porcine Rabbit 

5. 

7. rat 

Human 

I 

6. 

8. rat 

Porcine 

II 

7. Rat I 

8. Rat II 

Ribosomal Proteins 

Ribosomal Proteins 

0 5 10 15 20 25 Min. 


Mobile Phase: A: 0.1% Trifluoroacetic Acid 

Column: VYDAC B: 214TP54 Acetonitrile (C4, 5 µm, 4.6 mm i.d. x 250 mm) 

Gradient: 27 to 30% Time: ACN 0 with 25 0.1% TFA over 25 min 

%B: 27 30 

Detector: UV 

0 60 120 180 240 300 360 Min. 


Column: 

Mobile Phase: 

VYDAC 

A: 

214TP54 

0.1% 

(C4, 

Trifluoroacetic 

5 µm, 4.6 mm 

Acid 

i.d. x 250 mm) 

Eluent: 10-38% B: isopropanol Isopropanol with 0.1% TFA over 355 min 


%B: 10 38 

Detector: UV 

Tryptic Tryptic Digest of Digest Bovine Serum of Bovine Albumin Serum Albumin 



436 

0 10 20 30 40 50 60 70 Min. 

Column: Vydac ® Everest ® C18, 5µm, 150 x 4.6mm 

Columns: EVEREST (Part 238EV5415 No. 238EV5415) and 

Mobile Phase: four commercial A: 0.1% Trifluoroacetic 300 Å Acid in Water 

reversed-phase B: 0.085% columns Trifluoroacetic Acid in Acetonitrile 

Gradient: (all C18, Time: 5 µm, 4.6 0 mm i.d. 5 x 150 80 mm) 90 100 

Flow: 1.0 mL/min 

%B: 4 4 40 90 90 

Eluent: A = 0.1% v/v TFA in water 

Flow Rate: B = 0.085% 1.0mL/min v/v TFA in ACN 

Gradient: Detector: 4% B for UV 5 at min, 215nm 4-40% B in 75 min, 

40-90% B in 10 min, hold 90% B 

for 10 min 

Detector: www.discoverysciences.com 

Absorbance, 215 nm 

Temp: 22 °C 

Sample: 30 µL of a 5.8 µg/µL digest 

0 10 20 30 40 50 60 70 80 Min. 

Column: Vydac ® Everest ® C18, 5µm, 150 x 4.6mm 

Columns: EVEREST (Part 238EV5415 No. 238EV5415) and 

Mobile Phase: four commercial A: 0.1% Trifluoroacetic 300 Å Acid in Water 

reversed-phase columns 


(all C18, 5 µm, 4.6 mm i.d. x 150 mm) 



Eluent: A = 0.1% %B: v/v TFA 4 in water 4 40 90 90 

Flow Rate: B = 0.085% 1.0mL/min v/v TFA in ACN 

Detector: 

Gradient: 4% B for UV 5 at min, 215nm 4-40% B in 75 min, 

40-90% B in 10 min, hold 90% B 

for 10 min 

Detector: Absorbance, 215 nm 

Temp: 22 °C 

Sample: 30 µL of a 6 µg/µL digest



Peptides on 238MS C18 Column with Various Modifiers 

Peptides on 238MS C18 Column 

with Various Modifiers 

In order to demonstrate the effects of mobile phase modifiers and 

modifier concentrations, a mixture of six peptides was separated on 

a monomeric C18 MS column under identical gradient conditions 

with a variety of ion-pair reagents. Significant differences in selectivity 

arise depending on the concentrations and identities of the ions 

present in the mobile phase. Perfluorinated Acidic modifiers even 

at very low levels (0.005% w/v) produce increased retention. For 

this particular mixture, the best spread of peaks was obtained with 

heptafluorobutyric Acid (HFBA) at 0.02% (w/v). However, this might 

be expected to differ for other peptides. 

Column: 

Gradient: 

Flow Rate: 

Detector: 

Sample: 

Vydac ® Monomeric C18, 5µm, 250 x 4.6mm 

(Part No. 238MS54) 

10 to 40% Acetonitrile over 30 min for all 

chromatograms with constant concentration 

of modifier as indicated (w/v) 

1.0mL/min 

UV at 220nm 

Oxytocin, Bradykinin, Angiotensin II, Eledoisinrelated 

Peptide, Neurotensin, and Angiotensin I 

5 mM HOAc 

5 mM Formic Acid 

0.02% TFA 

0.005% TFA 

life science 

0.005% TFA + 0.1% HOAc 

0.005% TFA + 0.1% Formic Acid 

0.02% HFBA 

0.005% HFBA 

0.005% HFBA + 0.1% HOAc 

0 5 10 15 20 25 Min. 




437



Peptides 

Fast Protein Analysis 

5 

life science 

1 

2 

3 

4 

1. GY (238 Da) 

2. VYV (379 Da) 

3. Met Enkephalin (YGGFM, 573 Da) 

4. Leu Enkephalin (YGGFL, 555 Da) 

5. Angiotensin II (DRVYIHPF, 1045 Da) 

1 

2 

3 

4 

5 

6 

7 

1. Ribonuclease (37ng) 

2. Bovine Insulin (37ng) 

3. Cytochrome C (37ng) 

4. Lysozyme (4ng) 

5. Bovine Serum Albumin (37ng) 

6. Carbonic Anhydrase (37ng) 

7. Ovalbumin (37ng) 

0 0.2 0.4 0.6 0.8 1.0 1.2 Min. 

Column: Vydac ® ProZap C18, 500Å, 1.5µm, 10 x 2.1mm 


B: 0.085% Trifluoroacetic Acid:Acetonitrile (20:80) 

Gradient: Time: 0 0.1 0.7 1.1 1.2 

%B: 4 15 20 50 4 



0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Min. 

Column: Vydac ® ProZAP C18, 1.5µm, 10 x 2.1mm 




%B: 23 75 75 23 



Antibodies in Sheep Serum on ProZap 

Synthetic Peptides-10 Injections 

1 

1. Albumin 

2. IgG 

1. Contaminant Peptides/Impurities 

2. RGAGGLGLGK-amide (883 Da) 

3. Ac-RGAGGLGLGK-amide (926 Da) 

4. Ac-RGVGGLGLGK-amide (954 Da) 

5. Ac-RGVVGLGLGK-amide (996 Da) 

2 3 4 5 

1 

2 

0 1 2 3 4 5 6 Min. 



B: 0.085% Trifluoroacetic Acid:n-Propanol (10:90) 


%B: 5 75 75 5 



0 0.2 0.4 0.6 0.8 1.0 Min. 



B: 0.085% Trifluoroacetic Acid:Acetonitrile (20:80) 

Gradient: Time: 0 0.1 0.7 1.1 1.2 

%B: 4 15 20 50 4 



438 



Purification of Trityl-On Oligonucleotides 


Human Growth Hormone 

Human Growth Hormone 

3 

1. di-Desamido HGH 

1. di-desamido HGH 

2. mono-Desamido 2. mono-desamido HGH HGH 

3. Human Growth 3. human Hormone growth (HGH) hormone (HGH) 

Purification of Trityl-On Oligonucleotides 

36-mer 

1 

2 

0 7 14 21 28 35 42 Min. 


Mobile Column: Phase: VYDAC Isopropanol:0.01M 214ATP54 (C4, 5 µm, Tris 4.6 mm buffer, i.d. pH7.5 x 250 mm) (29:71) 

Flow Eluent: Rate: 29% isopropanol/71% 1.0mL/min 0.01 M Tris buffer, pH 7.5 

Detector: UV 

Separation of Protein / Peptide Mixture 

on Vydac 219TP Diphenyl Column 

Separation of Protein / Peptide Mixture on 

Vydac ® 219TP Diphenyl Column 

1 

3 

2 

4 

2 

5 

2 6 

7 

8 

1. Horse Cytochrome C 

Tryptic Digest Fragments 

2. Whale Myoglobin CNBr 

Digest Fragments 

3. Ribonuclease 

4. Cytochrome 

5. Lysozyme 

6. BSA 

7. Myoglobin 

8. Ovalbumin 

0 5 10 15 20 Min. 

Column: Vydac ® C4, 5µm, 250 x 10mm (Part No. 214TP510) 

Mobile Phase: A: 0.1M Triethylammonium Acetate, pH7.0 B: Acetonitrile 

Gradient: Column: VYDAC Time: 214TP510 0 14(C4, 5 µm, 10 mm i.d. x 250 mm) 

Gradient: 16 

B%: 

to 23% 

16 

ACN 

23 

in 0.1 M triethylammonium acetate, 

pH 7.0, over 14 minutes 

Flow Comparison Rate: of 5.0mL/min 

Polypeptide Separations on 

Detector: C18, C4 and Diphenyl UV Reversed-Phase Columns 

218TP 

(C18) 

214TP 

(C4) 

219TP 

(diphenyl) 

Comparison of Polypeptide Separations on 

C18, C4 and Diphenyl Reversed-Phase Columns 

1 

1 

1 

2 

2 

2 

1. H 

2. TH6 

life science 

1. H 

2. TH6 

0 10 20 30 40 50 60 70 Min. 

Column: Vydac ® Diphenyl, 5µm, 250 x 4.6mm 

Columns: VYDAC (Part 219TP54 No. (diphenyl, 219TP54) 5 µm, 4.6 mm i.d. x 250 mm) 

Gradient: Mobile Phase: 0-70% A: ACN 0.1% with Trifluoroacetic 0.1% TFA in 67.5 Acid min 

Sample: 1. horse B: cytochrome Acetonitrilec tryptic digest fragments; 

Gradient: 2. whale Time: myoglobin 0 CNBr 67.5 digest fragments; 

3. ribonuclease; 4. cytochrome c; 5. lysozyme; 6. BSA; 

B%: 0 70 

IgG 7. Purification myoglobin; from and 8. Bovine ovalbumin Serum 


Detector: UV 

IgG Purification from Bovine Serum 

2 

1. Serum Load Passthrough 

2. IgG 1. Serum Load Passthrough 

2. IgG 

0 5 10 15 20 25 30 Min. 

Column: Vydac ® C18, C4 and Diphenyl, all 5µm, 150 x 4.6mm 

(Part Nos. 218TP5415, 214TP5415, 219TP5415) 

Columns: Mobile Phase: VYDAC A: 218TP5415 0.1% Trifluoroacetic (C18), 214TP5415 Acid (C4) and 

219TP5415 B: Acetonitrile (diphenyl), all 5 µm, 4.6 mm i.d. x 150 mm 

Gradient: 10-90% ACN with 0.1% TFA over 30 min 


Sample: 18-residue helical peptide (1) and six-helix templateassembled 

B%: synthetic 10 90 protein (2) 

Sample: 18-Residue Helical Peptide (1) and 

Six-helix Template- assembled Synthetic Protein (2) 


Detector: UV 

Analysis of a Lipid Peptide 

Analysis of a Lipid Peptide 

1 

0 5 10 15 20 25 30 35 40 45 Min. 

0 10 20 30 40 50 60 Min. 

Column: Vydac ® Venture ® A RAVE Recombinant Protein A, 

Column: Vydac ® Diphenyl, 5µm, 250 x 4.6mm 

15-20µm, 50 x 4.6mm (Part No. 1520450) 

(Part No. 219TP54) 

Mobile Column: Phase: VENTURE A: 20mM A RAVE1520450 

Phosphate, pH7.4, 150mM Sodium Chloride Mobile Column: Phase: VYDAC A: 0.1% 219TP54 Trifluoroacetic (diphenyl, 5 µm, Acid 4.6 mm i.d. x 250 mm) 

(Recombinant Protein A, 15-20 µm, 4.6 mm i.d. x 50 mm) 

B: 0.1 M Sodium Citrate, pH3.2 


B: n-Propanol:Acetonitrile:0.1% Trifluoroacetic Acid (50:50) 

Flow: 

1.38 mL/min (linear velocity = 500 cm/hr) 

Temp: 45 °C 

Gradient: Equilibrate with A for 3 min, load sample for 9 min, 


Eluent: A = 20 mM phosphate, pH 7.4, 150 mM NaCl 

Eluent: A = 0.1% TFA (v/v) in water 

B = 0.1 wash M sodium with A citrate, for 12 pH min, 3.2elute with B for 3 min, 

B = 50:50 %B: n-propanol:ACN, 25 70 0.1% TFA (v/v) 

Gradient: Equilibrate re-equilibrate with A for with 3 min, A for load 6 sample min for 9 min, wash 

Flow Gradient: Rate: 25 to 1.5mL/min 

70% B over 45 minutes 

Flow Rate: with 1.38mL/min A for 12 min, elute (linear with velocity B for 3 = min, 500cm/hr) re-equilibrate 

Detector: UV 

Detector: with UV A for at 6280nm 

min 


more 

Sample: 

applications 

Commercial bovine serum diluted 1:20 with A and filtered 

View our complete through searchable a 0.45 chromatogram µm filter. Injection database volume at www.discoverysciences.com/chromdb/ 

= 12.4 mL. 


439



Purification of Synthetic Peptide, GnRH Antagonist 

Separation of Xenotropic Murine Leukemia Virus from 

Target Protein 

life science 

0 10 20 30 40 50 60 Min. 

Column: Vydac ® C18 , 15–20µm, 50mm i.d. x 30cm 

(Part No. 218TP15205030) 

Mobile Phase: Acetonitrile:Water/TEAP (Gradient) 

Sample: 1.2g of synthesis mixture 

300 mAU 

200 

100 

Column: 

Mobile Phase: 

XMuLV Virus 

was detected 

in this peak 

Ethanol Gradient 

XMuLV Virus 

was below the 

limit of detection 

in this peak 

0 0 50 100 150 200mL 

Vydac ® C4 , 20-30µm (Part No. 214TPB2030) 

Ethanol gradient 

Preparative Chromatography of Porcine Insulin 

Analyses of Starting Material and Pooled 

Fractions from Preparative Column 

Analyses 

A 280 

A) 26mg 

Starting 

material 

6x scale 

Fraction Collection 

from Run C 

B) 41.5mg 

Fraction 

1 

Fractions 

2-16 

Fractions 

17-26 

Pure pool 

(fract 2 -16) 

6x scale 

C) 60mg 

0 25 50 75 100 Min. 

40 50 60 70 80 90 Min. 

Column: 

Vydac ® C4, 10-15µm, 10 x 250mm (Part No. 214TP101510) 

A) Sample: 26mg partially purified porcine insulin in 30mL of 20% 

Acetonitrile/0.1% Trifluoroacetic acid, pumped on at 8mL/min 

Elution: 4mL/min 

Gradient: 20-35% Acetonitrile in 0.1% Trifluoroacetic acid over 90 min 

B) Sample: 41.5mg in 42mL of 20% Acetonitrile/0.1% Trifluoroacetic 

acid, pumped on at 8mL/min 


Gradient: 20-27% Acetonitrile in 35 min, hold 27% for 10 min 

followed by 27-28% in 20 min, 28%-35% in 10 min, and 

35-95% in 5 min, all in 0.1% Trifluoroacetic acid. 

C) Sample: 60mg in 60mL of 20% Acetonitrile/0.1% Trifluoroacetic 

acid, pumped on at 8mL/min 


Gradient: 20-27% Acetonitrile in 35 min, hold 27% for 10 min 

followed by 27-28.5% in 30 min, hold 28.5% for 20 min 

Impure pool 

(fract 1 & 

17-26) 

6x scale 

0 5 10 15 Min. 

Column: Vydac ® C4, 5µm, 4.6 x 150mm 

(Part No. 214TP5415) 

Samples: Starting material and pooled fractions 

of preparative run C, as indicated. 

Detection: UV at 215nm 

Elution: 

1.5mL/min 

Gradient: 30-33% Acetonitrile in 0.1% 

Trifluoroacetic acid over 15 min 

440 



1) Cystine 

2) Cysteine 

3) Methionine 


Amino Acids 

mPEG-SBA 

1 

10636 

1. n-Hydroxysuccinimidyl Ester 

of Methoxy Poly(ethylene 

glycol) Butanoic Acid 

1 

2 

Sulfur-Containing Native Amino Sulfur-Containing Acids Native Amino Acids 

9413 

3 

1. Cystine 

Column 2. Cysteine phase: GROM-SIL 120 ODS-4 HE, 5 µ 

Column 3. Methionine size: 250 x 4 mm 

Eluent: water 





Injection: 20 µl (50 ppm) 

life science 

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min. 

Column: Alltech ® ProSphere 300, 5µm, 150 x 4.6mm 

(Part No. 35560) 



%B: 30 30 80 



0 3 6 9 Min. 

Column: Grom Sil 120 ODS-4 HE, 5μm, 250 x 4mm 

(Part No. 5113626) 




1 

2 

3 

Thyroid Chemicals 

1. dl-Tyrosine 

2. 3-Iodo-Tyrosine 

3. 3,5- Diiodo-d-Tyrosine 

4 5 

8282 

4. dl-Thyroxine 

5. 3,3,5-Triiodo-l-Thyronine 

Phenylalanine Enantiomers 

O 

8770 

OH 

NH 2 

0 2 4 6 8 10 12 Min. 


(Part No. 32214) 




4 6 8 10 12 Min. 

Column: Astec Chirobiotic ® T, 250 x 4.6mm (Part No. 12053) 

Mobile Phase: Ethanol:Water (80:20) 



Tryptophan Derivatives 

Tryptophan Derivatives 

1 

2 

3 

1. 5-Hydroxytryptophan 

2. 5-Hydroxytryptamine 

3. Tryptophan 

1 

4 

3 

2 

5 

6 

7 

Amino Acids without Derivatization 

8 9 

10 

11 

13 

12 

14 

16 

15 

17 

1. Gly 

2. Ser/Asn 

3. Asp 

4. Gln 

5. Ala/Thr 

6. Glu 

7. Cys/Lys 

8. His 

9. Pro 

10. Arg 

11. Val 

12. Met 

13. Tyr 

14. Ile 

15. Leu 

16. Phe 

17. Trp 

0 2 4 6 8 10 12 14 16 18 20 22 Min. 

0 5 10 15 20 25 Min. 

Column: Vydac ® C18, 5µm, 250 x 4.6mm (Part No. 201SP54) Column: Alltech ® Prevail C18, 5µm, 250 x 4.6mm (Part No. 99210) 

Mobile Column: Phase: VYDAC A: 201SP54 50mM KH2PO4, (C18, 5 µm, pH4.59 4.6 mm i.d. x 250mm) 

Mobile Phase: A: 5mM Heptafluorobutyric Acid, 0.7% Trifluoroacetic Acid 

Flow: 

1.5 mL/min B: 90% Acetonitrile 


Gradient: Eluent: A = 50 Time: mM KH 2 PO 0 4 , pH 3 4.59 10 15 


B = 90% %B: ACN 1 1 15 35 

%B: 0 0 15 35 

Flow Gradient: Rate: Hold 1.5mL/min 

1% B for 3 min, then linear to 15% B over 7 min, 


Detector: 

then linear 

UV at 

to 

254nm 

35% B over 5 min 



Peaks: 1. 5-hydroxytryptophan, 2. 5-hydroxytryptamine, 


3. tryptophan 



441


life science 

Nucleic Acids 

1 

2 3 

4 

5 

Nucleosides 

1. Cytidine 

2. Uridine 

3. Xanthosine 

4. Guanosine 

5. Adenosine 

0 2 4 6 8 10 12 Min. 


(Part No. 87715) 

Mobile Phase: A: 0.03M Potassium Phosphate, pH3.2 



%B: 5 5 30 



10347 

1 

2 

Nucleosides 

0 5 10 15 20 Min. 

3 

4 

5 

1. Cytidine 

2. Uridine 

3. Xanthosine 

4. Guanosine 

5. Adenosine 


(Part No. 32246) 



%B: 5 5 30 



8245 

Nucleosides 

9410 

1 

Nucleic Acid Bases 

9411 

1 

2 

5 

1. Cytidine 

2. Uridine 

3. Xanthosine 

4. Guanosine 

5. Adenosine 

2 3 4 

5 

1. Cytosine 

2. Uracil 

3. Guanine 

4. Adenine 

5. Thymine 

3 

4 

0 2 4 6 8 Min. 





0 2 4 6 8 Min. 





442 



Miscellaneous 

Phospholipids 

10314 

1 

2 

3 

4 

1. 1,2-Phosphatidyl-ethanolamine 

2. Phosphatidyl-dl-glycerol 

3. Phosphatidylinositol 

4. 1,2-Phosphatidyl-l-serine 

5. Phosphatidylcholine 

6. Sphingomyelin 

5 6 


Mobile Phase: A: Isopropanol B: Hexane C: Water 


%B: 40 40 40 

%C: 2 8 8 



life science 

0 3 

6 9 12 15 18 21 24 Min. 

Monoaromatics 

Aromatic Acids 

2 

3 

7 

4 

5 6 

9 10 

1. Uracil 

2. Benzamide 

3. Benzyl Alcohol 

4. Phenol 

5. Methylbenzoate 

6. Acetophenone 

7. N,N-Diethyl-m-toluamide 

8. Toluene 

9. Benzophenone 

10. Biphenyl 

8982 

1 

3 

2 

4 5 

1. dl-4-Hydroxy-3-methoxy-mandelic Acid 

2. Homovanillic Acid 

3. Vanillic Acid 



7373 

1 

8 

0 1 

2 

3 

4 Min. 

0 1 2 3 4 5 6 7 8 9 10 11 Min. 


(Part No. 50573) 








1 

2 3 

4 

Benzyl Acids 

5 

9538 

1. 3-Amino-4-hydroxybenzoic Acid 

2. 4-Hydroxybenzoic Acid 


4. 4-Phenylbutyric Acid 

5. 5-Phenylbutyric Acid 

1 

2 3 

4 

5 

6 

7 

8 

10 

9 

11 

Alcohols 

12 

1. Glycerol 

2. Methanol 

3. Ethanol 

4. Isopropanol 

5. tert-Butanol 

6. Propanol 

7. sec-Butanol 

8. Isobutanol 

9. tert-Pentanol 

10. Butanol 

11. Isopentanol 

12. Pentanol 

5440 

0 2 4 6 8 10 12 14 Min. 

Column: Alltech ® Prevail Organic Acid, 5µm, 150 x 4.6mm 

(Part No. 88640) 




0 4 8 12 16 20 24 28 Min. 

Column: Alltech ® OA-1000, 9µm, 300 x 6.5mm (Part No. 9046) 


Flow Rate: 0.8ml/min 


Detector: RI 




443


Pesticides 

1 

Organophosphates 

3 

10346 

1. Methyl Parathion 

2. Dichlorvos 

3. Ethoprophos 

4. Azinphos 

1 

Organochlorines 

1. Methoxychlor 

2. Dieldrin 

3. Heptachlor 

4. 4,4-DDT 

8437 

environmental 

0 4 

2 

8 12 16 20 24 Min. 


(Part No. 87715) 




4 

0 3 

2 

3 

6 9 12 Min. 


(Part No. 32043) 

Mobile Phase: 0.025M K2HPO4, pH7.0:Methanol (25:75) 



4 

1 

2 

3 

Triazine Herbicides 

4 5 

6 

7 

1. Simazine 

2. Simetryn 

3. Prometon 

4. Atrazine 

5. Ametryn 

6. Prometryn 

7. Terbutryn 

8671 

1 

2 

3 

Herbicides 

4 

1. Fenuron 

2. Monuron 

3. Diuron 

4. Linuron 

10355 

0 1 2 3 4 5 Min. 

Column: Alltech ® Platinum EPS C18, 

1.5µm, 33 x 7mm Rocket (Part No. 50575) 




0 

1 2 3 4 5 6 7 8 Min. 


(Part No. 87769) 




Carbamate and Urea Pesticides 

10739 

1 

Bipyridylium Herbicides 

8439 

1 

2 

4 

3 

5 

6 

7 

1. Fenuron 

2. Monuron 

3. Fluometuron 

4. Diuron 

5. Methiocarb 

6. Linuron 

7. Neburon 

2 

1. Diquat 

2. Paraquat 

0 2 4 6 8 10 12 14 16 18 20 Min. 


(Part No. 5138812) 

Mobile Phase: A: 0.025M KH2PO4, pH3.2 



%B: 30 60 



0 2 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

4 6 8 Min. 

Alltech ® Platinum Silica 100Å 3µm, 

30mm x 4.6mm (Custom) 

0.01M Tetramethylammonium Hydroxide and 

0.15M Ammonium Sulfate in Water, pH3.0 with 

5M Sulfuric Acid 

0.5mL/min 

UV at 310nm until 3 minutes 

UV at 260nm after 3 minutes 

444 



1 

Organochlorine Pesticides 

0 2 4 6 8 10 12 14 Min. 


(Part No. 5138812) 

Mobile Phase: Methanol:0.025M K2HPO4, pH7.0 (75:25) 



2 

10740 


2. 4,4-DDT 

1 

2 

3 4 5 

Triazine Pesticides 

6 7 89 

0 1 2 3 4 5 6 7 8 9 Min. 

Column: Vydac ® Denali ® 238DE C18, 3µm, 100 x 4.6mm 

(Part No. 238DE3410) 

Mobile Phase: A: 25mM Potassium Phosphate, pH6.4 



%B: 40 40 63 63 



10580 

1. Simazine 

2. Atraton 

3. Simetryn 

4. Atrazine 

5. Prometon 

6. Ametryn 

7. Propazine 

8. Prometryn 

9. Terbutryn 

environmental 

2 

1 

3 

Analysis of Pesticides 

5 

4 6 

78 9 

12 14 

13 

15 

11 

10 

0 3 6 9 12 Min. 

1. Methomyl 

2. Oxamyl 

3. Fenurone 

4. Monurone 

5. Carburone 

6. Propoxur 

7. Carbaryl 

8. Fluometurone 

9. Diurone 

10. Propham 

11. Sidurone 

12. Linurone 

13. Chlorpropham 

14. Barban 

15. Neburone 

1 

2 

3 

Atrazine Herbicides 

Atrazine Herbicides 

0 2 4 6 8 10 Min. 

4 

1. Atazine Desisopropyl 

2. Atrazine Desethyl 

3. Simazine 

4. Propazine 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Grom Sil 100 Octyl-4 FE, 3μm, 150 x 4mm 

A: Water B: Acetonitrile 

1.5mL/min 

UV at 240nm 

Column: GENESIS Jones FM15965E Genesis (CN, ® CN, 4 µm, 4μm, 4.6150 mm i.d. x 4.6mm x 150 mm) 

Flow: 

1.0 mL/min 


Eluent: Isocratic, 15% ACN/0.05% TCA in water (pH 3.0) 

Detection: 

Mobile Phase: 

Absorbance, 

Acetonitrile:0.05% 

254 nm 

Trichloroacetic Acid in Water, pH3.0 

Peaks: 1. Atazine (15:85) desisopropyl, 2. Atrazine desethyl, 3. Simazine, 

Flow Rate: 4. Propazine 1.0mL/min 


Separation of Chlorophenoxyacid Herbicide Mixtures 

Separation of Chlorophenoxy Acid Herbicide Mixtures 

1 

Benzenes 

Benzenes 

10713 

2 

3 4 8 

5 6 7 

1. picloram 

2. chloramben 

3. dicamba 

4. bentazon 

5. 2,4-D 

6. dichlorprop 

7. 2,4,5-TP 

8. acifluorphen 

1. Picloram 

2. Chloramben 

3. Dicamba 

4. Bentazon 

5. 2,4-D 

6. Dichlorprop 

7. 2,4,5-TP 

8. Acifluorphen 

1 

2 

3 

4 

1. Thiourea 

2. Toluene 


4. Butylbenzene 

0 1 2 3 4 5 Min. 

0 0.5 1 1.5 2 Min. 

Column: Vydac ® Denali ® , 3μm, 100 x 4mm (Part No. 238DE3410) Column: Grace ® VisionHT C18, 1.5µm, 50 x 2.0mm 

Mobile Phase: A: 0.5% Phosphoric Acid Buffer, pH1.8; B: Acetonitrile 

(Part No. 5139605) 

Gradient: Column: DENALI Time: 238DE3410 0 5(3 µm, 4.6 mm i.d. x 100 mm) 

Mobile Phase: 20mM Potassium Phosphate, pH2.5:Acetonitrile (45:55) 

Flow: 

1.0 mL/min 

%B: 50 90 


Mixture A Eluent: A = 0.5% phosphoric acid buffer, pH 1.8; B = ACN 

Flow Rate: 

Gradient: 

1.0mL/min 

50 to 90% B over 5 min 


Detector: 

Temp: 23 °CUV at 225nm 


more Samples: applications 2 µL herbicide mixtures, 0.3 µg/mL each compound 



445


PAHs 

Polyaromatic Hydrocarbons 

9400 

environmental 

4 6 11 7 

12 

5 

14 

16 

13 

8 

9 

1 3 

15 

17 

2 

10 

0 2 4 6 8 10 12 14 16 18 20 Min. 

1. Benzene 



4. Fluorene 



7. Anthracene 


9. Pyrene 


11. Chrysene 




15. Dibenz[a,h]anthracene 

16. Benzo[g,h,i]perylene 

17. Indeno[1,2,3,c,d]pyrene 




%B: 72 72 100 



Beyond the EPA 16 Priority-Pollutant PAHs 

1 

2 

3 4 5 

6 

7 

8 

9 

10 

18 

1112 13 1415 16 17 

19 

20 

23 

22 

21 

0 4 8 12 16 20 24 28 32 Min. 






6. Fluorene 


8. Anthracene 


10. Pyrene 

11. Benzo[c]phenanthrene 

12. Cyclopenta[c,d]pyrene 


14. Chrysene 

15. Benzo[b]naptho[2,1-d]thiopen 

16. 7,12-Dimethylbenz[a]anthracene 

17. Benzo[e]pyrene 



20. Benz[a]pyrene 







%B: 50 100 



2. Acenapthylene 


4. Fluorene 


6. Anthracene 

12 3 

4 

5 

Fast Polyaromatic Hydrocarbons 


8. Pyrene 

9. Benzo[a]anthracene 

10. Chrysene 



6 

7 8 

9 

10 

11 

13 16 

12 15 

14 

10757 


14. Dibenzo[a,h]anthracene 



0 1 2 3 4 5 6 Min. 

Column: Alltech ® Alltima HP C18 HiLoad, 3µm, 53 x 7mm 

(Part No. 87690) 


Gradient: Time: 0 0.1 5 

%B: 40 40 100 



0 

Nitroaromatics 

1 

Nitroaromatics (Normal-Phase) 

2 

3 

4 

1 2 3 4 5 6 7 8 Min. 


(Part No. 87769) 

Mobile Phase: Hexane:Isopropanol (85:15) 



10358 

1. Nitrobenzene 

2. 2,6-Dinitrotoluene 

3. 1,3-Dinitrobenzene 


446 



Water Pollutants 

Semipolar & Highly Hydrophilic Waste Water Contaminants 

0 

1 

2 

3 

4 

5 

6 7 8 

9 

10 

1112 

13 

14 

16 

15 17 

10 20 30 40 50 Min. 


(Part No. 32246) 

Mobile Phase: A: 0.03M Monosodium Phosphate, pH2.5 

B: Acetonitrile:Methanol:Water (40:50:10) 

Gradient: Time: 0 3 13 20 25 30 40 45 55 

%B: 5 5 10 10 20 40 75 80 80 



18 

19 

8447 

1. 4-Amino-5-hydroxy-2,7-naphthalene-disulfonic Acid 

2. Hydroquinone 

3. Benzylamine 

4. 3'-Aminoacetophenone 

5. Phenol 

6. 4-Hydroxyphenylacetic Acid 

7. 1-Naphthalene Sulfonic Acid 

8. 2,4-Dihydroxybenzoic Acid 

9. 1-Naphthalene Sulfonic Acid Degradant 

10. 4-Hydroxy-3-methoxybenzoic Acid 

11. Phenylacetic Acid 

12. m-Cresol 

13. 4-Hydroxycinnamic Acid 

14. trans-Cinnamaldehyde 

15. trans-Cinnamaldehyde Degradant 

16. trans-Cinnamaldehyde Degradant 

17. 2-Naphthalenethiol Degradant 

18. 2-Naphthalenethiol 

19. 2-Naphthalenethiol Degradant 

environmental 

Explosives 

Nitroaromatic and Nitroamine Explosives 

1 

2 

3 

4 

5 

6 

7 

1. HMX 

2. RDX 

3. TNB 

4. DNB 

5. Tetryl 

6. NB 

7. TNT 

8. 4-A-DNT 

9. 2,6DNT 

10. 2,4DNT 

11. 2-NT 

12. 3-NT 

13. 4-NT 

8 9 10 11 12 

13 

8279 

1. HMX 

2. RDX 

3. 1,3,5-TNB 

1 2 

3 

Explosive Analysis, EPA Method 8330 

4 

5 

4. 1,3-DNB 

5. Tetryl 

6. Nitrobenzene (NB) 

7. 2,4,6-TNT 

6 

7 

8 9 1011 

12 13 14 

10511 

8. 4-Amino-2,6-Dinitrotoluene 

(4-AM-DNT) 

9. 2-Amino-4,6-Dinitrotoluene 

(2-Am-DNT) 

10. 2,6-DNT 

11. 2,4-DNT 

12. 2-NT 

13. 4-NT 

14. 3-NT 

0 2 4 6 8 10 12 14 16 18 20 22 Min. 


(Part No. 32064) 




0 5 10 15 20 25 30 35 40 Min. 

Column: Alltech ® Adsorbosphere UHS C18, 5µm, 150 x 4.6mm 

(Part No. 288118) 




Phenols 

1 

2 

Phenols 

3 4 5 

10707 

1. Phenol 





Separation of EPA 604 Phenols Mixture 

1 

2 

3 

4 

7 

5 

6 8 

9 

10 11 

1. Phenol 






7. 4-Chloro-3-Methylphenol 


9. 2-Methyl-4,6-Dinitrophenol 


11. Pentachlorophenol 

0 2 4 6 8 10 12 14 Min. 


(Part No. 5138810) 

Mobile Phase: A: 1% Glacial Acetic Acid in Water 

B: 1% Glacial Acetic Acid in Methanol 


%B: 45 45 60 80 



0 1 2 3 4 5 6 7 8 9 10 11 12 13 Min. 


Mobile Phase: A: 1% Acetic Acid in Water 

B: 1% Acetic Acid in Acetonitrile 


%B: 35 63 95 95 






447


Fullerenes 

“Bucky Balls” 

Buckminsterfullerenes 

2 

7210 

Buckminsterfullerenes 

1. C70 

2. C60 

1 

C 60 C 70 

C 76 C84 

miscellaneous 

0 30 60 90 120 150 180 Min. 

0 5 10 15 20 Min. 

Column: Jordi DVB, 500Å, 500 x 10mm (6) (Part No. 100567) 1) trans-1-C62(COOEt)4 

Column: Vydac ® C4, 5μm, 250 x 10mm (Part No. 201TP510) 

Mobile Phase: Trichlorobenzene 

2) trans-2-C62(COOEt)4 

Mobile Column: Phase: VYDAC Acetonitrile:Toluene 201TP510 (C4, 5 µm, (50:50) 10 mm i.d. x 250 mm) 



Flow Eluent: Rate: Isocratic, 5mL/min 1:1 ACN/toluene 


Flow: 5 mL/min 


Detector: RI 

5) e-C62(COOEt)4 

Cooking Oil 

Regioisomeric Fullerene Derivatives Regioisomeric Fullerene Derivatives 

5 Used Cooking Oil 

7215 

1 

2 

3 

4 

5 

1. trans-1-C62(COOEt)4 




5. e-C62(COOEt)4 

Column phase: GROM-SIL 100 Norm Ph-1 ST, 5 µm 


Eluent: toluene 




Detection (UV): 340 nm, 0.084 

6 

AUFS 

Injection: 5 µl (50 µg/ml) 3 

1 2 7 8 

1. Polymerized 

2. Polymerized 

3. Triglycerides 

4. MW 1800-3000 

5. Triglycerides MW 930 

6. Diglycerides MW 620 

7. Monoglycerides MW 400 

8. Free Fatty Acids MW 300 

0 1 2 3 4 5 6 7 8 9 Min. 

Column: Grom Sil 100 Norm Ph-1 ST, 5μm, 250 x 4mm 

Mobile Phase: Toluene 



0 10 20 30 40 Min. 

Column: Jordi DVB, 10 3 Å, 500Å, 500mm x 10mm (2) 

(Part No.s 100571, 100567) 

Mobile Phase: Tetrahydrofuran 



Surfactants 

Microbore Analysis of Glucopyranosides 

1. n-octyl Glucopyranoside (50ng on column) 

2. n-decyl Glucopyranoside (50ng on column) 

3. n-dodecyl Glucopyranoside (50ng on column) 

10310 

1 

2 

3 

0 1 

2 

3 

4 5 6 7 8 Min. 





448 



Polymers 

Polyurethane Curatives 

7472 

Polystyrene 

7213 

1 

2 

1. Polytetramethylene Glycol 

2. Ethecure 300 

1 

2 3 4 5 6 7 

8 

9 

10 

1. 8.42M 

2. 1.3M 

3. 791K 

4. 354K 

5. 98.9K 

6. 43K 

7. 10.1K 

8. 2.6K 

9. 530K 

10. 120K 

70 100 130 160 Min. 

Column: Jordi DVB, 500Å, 500mm x 10mm (6) 

(Part No. 100567) 

Mobile Phase: Chloroform 



Detector: RI 

0 5 10 15 20 25 30 Min. 

Column: Jordi DVB, Mixed Bed Linear, 500mm x 10mm 

(Part No. 100583) 

Mobile Phase: Tetrahydrofuran 



Detector: RI 

miscellaneous 

1 

2 

9 

8 

3 4 567 

10 1112 13 

14 15 

16 

17 

18 

19 

20 

2122 

Polyethylene and Hydrocarbon Standards 

2425 

23 

26 2728 29 

0 30 60 90 120 150 180 Min. 

30 

31 

32 

1. PE-32000 MW 

2. PE-2155 MW 

3. C58 

4. C56 

5. C54 

6. C52 

7. C50 

8. C48 

9. C46 

10. C44 

11. C42 

12. C40 

13. C38 

14. C36 

15. C34 

16. C32 

17. C30 

18. C28 

19. C26 

20. C24 

21. C22 

22. C20 

23. C18 

24. C16 

25. C14 

26. C12 

27. C10 

28. C9 

29. C8 

30. C7 

31. C6 

32. C5 

7220 

Column: Jordi DVB, 500Å, 500mm x 10mm (6) 

(Part No. 100567) 

Mobile Phase: Trichlorobenzene 



Detector: RI 

Tween 80 

Dimethicone 

7809 

8636 

0 5 10 15 20 25 30 Min. 0 4 8 12 16 20 Min. 


(Part No. 88056) 





Mobile Phase: A: Acetonitrile B: Chloroform 


%B: 25 75 






449


Polymers 

Polyethylene Glycol (MW=600) 

8442 

Phenones/Fast Gradient Column Formats 

1 

2 3 

Phenone Homologs 

4 5 

7 

6 

8 

9 

9467 

1. Uracil 



4. Propiophenone 

5. Butyrophenone 



8. Heptanophenone 

9. Octanophenone 

miscellaneous 

0 5 10 15 20 25 30 35 Min. 

Column: Alltech ® Adsorbosphere C8, 5µm, 250 x 4.6mm 

(Part No. 287112) 

Mobile Phase: A: Methanol B: Water 


%B: 60 20 



1 

2 3 

4 5 

6 

0 1 2 3 Min. 

Columns: Alltech ® Alltima C18, 3µm, 10 x 2.1mm Expedite 

(Part No. 43836) 




%B: 5 100 



1 

2 


3 

7 

4 5 

8 

9 


6 

7 

8 

9 

9471 

1. Uracil 









9460 

1. Uracil 









0 1 2 3 Min. 


(Part No. 43451) 




%B: 5 100 



0 1 2 3 Min. 


(Part No. 43837) 




%B: 5 100 



1 

1 

2 

2 3 


4 5 

7 

6 

3 

4 5 

8 9 


6 

7 

8 

9 

9468 

1. Uracil 









9462 

1. Uracil 









0 1 2 3 Min. 

Column: Alltech ® Prevail C18, 3µm, 20 x 2.1mm, Expedite 

(Part No. 43827) 




%B: 0 100 100 



0 1 2 3 Min. 

Column: Alltech ® Alltima C18, 3µm, 20 x 2.1mm Expedite 

(Part No. 43803) 




%B: 5 100 



450 



Biofuels 

Triacylglycerols in Canola Oil 

6 

10749 

Triacylglycerols in Red Palm Oil 

7 8 

10750 

1 

2 

3 4 5 

0 5 10 15 20 25 Min. 

1. LLnLn 

2. LLLn 

3. OLnL 

4. LLO 

5. LOO 

6. PLO 

7. OOO 

8. POO 

9. POP 

10. PPP 


(Part No. 87698) 



%B: 30 70 70 30 30 



6 

5 

4 

1 2 3 

0 5 10 15 20 25 Min. 

9 

10 

11 

12 13 

1. PLL 

2. LOO 

3. PLO 

4. PLP 

5. MOP 

6. OOO 

7. POO 

8. POP 

9. SOO 

10. PPP 

11. POS 

12. PPS 

13. SOS 


(Part No. 87698) 



%B: 30 70 70 30 30 



miscellaneous 

4 6 7 9 

1. Mono-acylglycerol 

(MAG) 

2. MAG 

3. MAG 

8 

3 

11 

5 

1 2 10 

0 5 10 15 20 25 30 Min. 

Triacylglycerols in Used Chinese Frying Oil 

3 

10751 

6 

3 

1 

2 

7 

8 

10 

4 

9 

5 

7 8 9 10 

451 

Biodiesel in Spec with EN14105 Method 

4. Fatty Acid Methyl Ester 

(FAME) 

5. MAG 

6. FAME 

7. FAME 

8. MAG 

9. FAME 

10. Di-acylglycerol (DAG) 

11. Tri-acylglycerol (TAG) 

10644 


(Part No. 87698) 



%B: 0 15 70 70 0 



Triacylglycerols in Soy Oil 

10748 

11 12 13 14 

15 

1. LLnLn 

2. LLLn 

3. LLL 

4. OLLn 

5. PLLn 

6. LLO 

7. PLL 

8. LOO 

9. LLS 

10. PLO 

11. PLP 

12. OOO 

13. SLO 

14. POO 

15. POP 

6 

7 

2 10 9 

8 

4 

12 

11 13 

5 14 

1 15 

1. LLnLn 

2. LLLn 

3. LLL 

4. OLLn 

5. PLLn 

6. LLO 

7. PLL 

8. LLO 

9. LLS 

10. PLO 

11. PLP 

12. OOO 

13. SLO 

14. POO 

15. POP 

0 5 10 15 20 25 Min. 


(Part No. 87698) 



%B: 30 70 70 30 30 



0 5 10 15 20 25 Min. 


(Part No. 87698) 



%B: 30 70 70 30 30 





ww.discoverysciences.com


Biofuels 

Triacylglycerols in Lard 

2 

10752 

Fire Retardants 

Brominated Flame Retardants 

10261 

1 

3 

1. PPO 

2. PPP 

3. POS 

4. SSS 

1 2 

1. Hexabromo 

Cyclododecane 

2. Tetrabromo 

Bisphenol A (Allyl Ether) 

miscellaneous 

4 

0 5 10 15 20 25 Min. 


(Part No. 87698) 



%B: 30 70 70 30 30 



0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min. 





Standards 

5 

7 

3 

6 

4 

1 

2 

Standards Mix 

10696 

1. Nicotine 

2. Quinine 



5. 2,4, Dichlorophenoxy Acetic Acid 

6. 2,4, Dichlorophenoxy Propionic Acid 

7. 2,4, Dichlorophenoxy Butyric Acid 

1 

2 

3 

4 

Acids, Bases and Neutrals 

1. Uracil 


3. Phenylvaleric Acid 

4. Toluene 

10695 

0 2 4 6 8 Min. 




Dyes 

1 2 3 

4 

5 

Color Additives - Dyes 

6 

7 

8 

9 10 11 

0 10 20 30 40 50 Min. 

Column: 

1. Tartrazine 

2. Amaranth 

3. Indigo Carmine 

4. New Coccine 

5. Sunset Yellow 

6. Fast Green 

7. Brilliant Blue 

8. Erythosine 

9. Acid Red 

10. Phloxine 

11. Rosé Bengale 

Grom Sil 120 ODS-5 ST, 5μm, 150 x 4mm 

(Part No. 5113642) 

Mobile Phase: A: 10mM Ammonium Phosphate, pH6.0:Methanol (90:10) 

B: 10mM Ammonium Phosphate, pH6.0:Methanol (20:30) 


%B: 0 100 100 

Flow Rate: 

Detector: 

0.8mL/min 

UV at 254nm 

0 2 4 6 8 Min. 




Solvents 

1 

2 

Caprolactams 

Caprolactams 

3 

1. N-Methylol Caprolactam 

2. Caprolactam 

3. N,N-Methylene-bis(Caprolactam) 

4. N,N-oxy-bis(Methylene)--Caprolactam 

0 2 4 6 8 10 12 Min. 

Column: GENESIS Jones FM15960E Genesis (C18, , 4μm, 4 µm, 150 4.6 x mm 4.6mm i.d. x (Part 150 mm) No. FM15960E) 

Flow: Mobile Phase: 1.0 mL/min Acetonitrile:20mM Phosphate, pH6.8 (22:78) 

Eluent: Flow Rate: Isocratic, 1.0mL/min 22% ACN in 20 mM phosphate, pH 6.8 

Detection: Detector: Absorbance, UV at 205nm 

Peaks: 1. N-methylol caprolactam, 2. Caprolactam, 

3. N,N-methylene-bis(caprolactam), 

4. N,N-oxy-bis(methylene)--caprolactam 

4 

452 



Fuels 

Petrochemicals 

Chelators 

Metal Chelators 

1 

8584 

1. Aliphatics 

2. Mono-aromatics 

3. Di-aromatics 

4. Polyaromatics 

2 

1. 2,2’-Dipyridyl 

2. 8-Hydroxyquinoline 

2 3 

1 4 

0 5 10 15 20 25 30 35 40 Min. 

Column: Jones Genesis ® Petro XL, 3µm, 250 x 4.6mm 


Mobile Phase: Hexane 



0 2 

4 6 Min. 


Mobile Phase: 0.025M K2HPO4, pH7.0:Tetrahydrofuran: 

Methanol (50:25:25) 



1) ?-Cyclodextrin 



miscellaneous 

Paraffins 

Cyclodextrins 

Paraffin Analysis 

Paraffin C20-C30 

10429 

1 

2 

3 

Cyclodextrins 

Cyclodextrins 

1. γ-Cyclodextrin 

2. α-Cyclodextrin 

3. β-Cyclodextrin Column phase: GROM-SIL 120 O 


Eluent:: water / methanol = 95 / 5 


Pressure: 8.8 MPa 


Detection: RI 

Injection: 5 µl (20 - 60 µg) 

0 2 4 6 8 10 12 14 16 18 20 22 Min. 


(Part No. 87685) 

Mobile Phase: Acetonitrile:Dichloromethane (60:40) 



0 5 10 15 20 25 Min. 

Column: Grom Sil 120 ODS-5 ST, 5μm, 150 x 4mm 

(Part No. 5113642) 



Detector: RI 




453

ic applications 

anions 

Anions 

1 

2 

3 

Anions in Reagent Grade Water 

by EPA Method 300.1 

4 

5 

6 7 

8 

0 4 8 12 16 20 24 Min. 

Column: Alltech ® Novosep A-2 Anion, 250 x 4mm (Part No. 51410) 

Mobile Phase: 3.6mM Sodium Carbonate 



Detector: Suppressed Conductivity 

9 

10 

1. Fluoride 

2. Chlorite 

3. Bromate 

4. Chloride 

5. Nitrite 

6. Bromide 

7. Chlorate 

8. Nitrate 

9. Phosphate 

10. Sulfate 

10120 

1 

Anions in High Ionic Strength Water by 

EPA Method 300.1 

2 

3 

4 5 

6 7 

8 

9 10 11 

1. Fluoride 

2. Chlorite 

3. Bromate 

4. Chloride 

5. Nitrite-N 

6. Bromide 

7. Chlorate 

8. Dichloroacetate 

9. Nitrate-N 

10. Phosphate-P 

11. Sulfate 

10121 

0 2 4 6 8 10 12 14 16 18 20 22 24 26 Min. 

Column: Alltech ® Novosep A-2 Anion, 250 x 4mm (Part No. 51410) 





Trace Bromate with Post Column Derivatization 

and UV Detection, EPA Method 317.0 

Arsenite and Arsenate 

10123 

9178 

2 

1. Chlorite 

2. Bromate 

3. Nitrite 

1 

2 

1. Arsenite 

2. Arsenate 

3 

1 

0 2 4 6 8 10 12 14 16 18 20 22 24 Min. 

0 5 10 Min. 

Column: Alltech ® Novosep A-2 Anion, 250 x 4mm (Part No. 51410) Column: Alltech ® Allsep Anion, 100 x 4.6mm (Part No. 51207) 


Mobile Phase: 4mM p-Hydroxybenzoic Acid, pH8.5 


with Lithium Hydroxide 



Reagent: o-Dianisidine 



Diet Cola 

1 

Toothpaste 

8458 8464 

1 

1. Aspartame 

2. Chloride 

3. Nitrate 

4. Sulfate 

4 

1. Fluoride 

2. Chloride 

3. Phosphate 

4. Monofluorophosphate 

2 

3 

4 

2 3 

0 2 

4 

6 8 10 Min. 0 4 

8 12 16 Min. 

Column: Alltech ® Allsep Anion, 100 x 4.6mm (Part No. 51207) 

Mobile Phase: 4mM Phthalic Acid, pH4.2 with Lithium Hydroxide 




Mobile Phase: 0.85mM Sodium Bicarbonate/0.9mM Sodium Carbonate 





2 

Anions 

13033 

2 

Bakery Additives 

13040 

1 

3 

4 

5 

7 

1. Fluoride 

2. Chloride 

3. Nitrite 

4. Bromide 

5. Nitrate 

6. Phosphate 

7. Sulfate 

1 

1. Iodate 

2. Bromate 

6 

0 2 4 6 8 10 12 14 16 18 20 22 24 Min. 0 1 2 3 4 5 6 7 8 9 Min. 

Column: Alltech ® Allsep Anion, 100 x 4.6mm (Part No. 51207) Column: Alltech ® Allsep Anion, 100 x 4.6mm (Part No. 51207) 

Mobile Phase: 0.85mM Sodium Bicarbonate/0.9mM Sodium Carbonate Mobile Phase: 0.85mM NaHCO3/ 0.9mM NaHCO3 





anions 

Selenite, Selenate, Arsenate, 

Tungstate and Molybdate 

Selenite, Selenate, Tungstate, Molybdate and 

Seven Common Anions 

1 

2 

1. Fluoride 

2. Chloride 

3. Nitrite 

4. Bromide 

5. Nitrate 

6. Phosphate 

7. Selenite 

8. Sulfate 

9. Arsenate 

10. Selenate 

11. Tungstate 

12. Molybdate 

8610 

1 

2 3 

4 5 

1. Fluoride 

2. Chloride 

3. Nitrite 

4. Bromide 

5. Nitrate 

6. Phosphate 

7. Selenite 

8. Sulfate 

9. Selenate 

10. Tungstate 

11. Molybdate 

9176 

3 

6 7 

8 9 

10 11 

4 5 

6 

7 8 9 10 11 12 

0 10 20 30 40 Min. 0 5 10 15 20 25 Min. 

Column: Alltech ® Allsep Anion, 150 x 4.6mm (Part No. 51209) Column: Alltech ® Allsep A-2, 50 x 4.6mm (Part No. 51221) 

Mobile Phase: 0.85mM Sodium Bicarbonate/0.9mM Sodium Carbonate Mobile Phase: 2.1mM Sodium Bicarbonate/1.6mM Sodium Carbonate 





Anions with Phthalic Acid Mobile Phase 

2 

8432 

1 

4 

Oxalate in Urine 

8451 

1 

3 

4 5 

6 

1. Fluoride 

2. Chloride 

3. Nitrite 

4. Bromide 

5. Nitrate 

6. Sulfate 

3 

1. Chloride 

2. Nitrate 

3. Phosphate 

4. Sulfate 

5. Oxalate 

2 

5 

0 2 

4 6 8 10 Min. 0 4 


Mobile Phase: 4mM Phthalic Acid, pH4.2 with Lithium Hydroxide 




8 12 16 20 Min. 








455


Anions 

1 2 

Common Anions Rapid Analysis 

1. Fluoride 

2. Chloride 

3. Nitrite 

4. Bromide 

5. Nitrate 

6. Phosphate 

7. Sulfate 

9174 

1 

2 3 4 

5 

6 


1. Borate 

2. Silicate 

3. Formate 

4. Sulfide 

5. Chloride 

6. Cyanide 

5697 

3 

4 

5 

6 

7 

anions 

0 2 4 6 8 10 Min. 

Column: Alltech ® Allsep A-2, 50 x 4.6mm (Part No. 51221) 




0 9 18 Min. 

Column: Alltech ® Anion/R, 10µm, 250 x 4.1mm (Part No. 269029) 

Mobile Phase: 4mM Sodium Hydroxide/0.1mM Sodium Benzoate 



1 

Organic and Inorganic Anions 


5 

3 

4 

2 

6 

7 

8 

9 

10 

11 

1. Fluoride 

2. Acetate 

3. Formate 

4. Chlorite 

5. Chloride 

6. Nitrite 

7. Bromide 

8. Chlorate 

9. Nitrate 

10. Phosphate 

11. Sulfate 

8747 

1 

2 3 4 

5 

1. Fluoride 

2. Acetate 

3. Formate 

4. Chlorite 

5. Chloride 

13041 

0 6 12 18 24 Min. 0 1 2 3 4 5 6 7 8 9 Min. 

Column: Alltech ® Allsep A-2 Anion, 100 x 4.6mm (Part No. 51219) Column: Alltech ® Allsep A-2 Anion, 100 x 4.6mm (Part No. 51219) 

Mobile Phase: 2.1mM Sodium Bicarbonate/1.6mM Sodium Carbonate Mobile Phase: 2.1mM Sodium Bicarbonate/1.6mM Sodium Carbonate 





Selenite, Selenate, and Other Anions 

Separation of Sulfite and Sulfate 

8611 6316 

1 

2 3 4 5 6 

7 

8 

9 

1. Fluoride 

2. Chloride 

3. Nitrite 

4. Bromide 

5. Nitrate 

6. Selenite 

7. Phosphate 

8. Sulfate 

9. Selenate 

1 

2 

3 4 5 6 

7 

8 

9 

1. Water 

2. Fluoride 

3. Chloride 

4. Nitrite 

5. Bromide 

6. Nitrate 

7. Phosphate 

8. Sulfite 

9. Sulfate 

0 4 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

8 12 16 Min. 

Alltech ® Sarasep AN1, 9µm, 250 x 4.6mm 

(Part No. 38140) 

0.75mM Sodium Bicarbonate, 2.2mM Sodium Carbonate 

1.0mL/min 


0 2 4 6 8 10 12 14 16 Min. 

Column: Alltech ® ICSep AN1, 9µm, 250 x 4.6mm 

(Part No. 38140) 

Mobile Phase: 1.8mM Sodium Bicarbonate, 1.8mM Sodium Carbonate 





3 

Anions with pHBA Mobile Phase 

8750 

2 

7 

Drinking Water 

8954 

1 

2 

4 

5 6 

7 8 

1. Fluoride 

2. Carbonate 

3. Chloride 

4. Nitrite 

5. Bromide 

6. Nitrate 

7. Phosphate 

8. Sulfate 

9. System Peak 

1 

1. Fluoride 

2. Chloride 

3. Bicarbonate 

4. Nitrite 

5. Nitrate 

6. Phosphate 

7. Sulfate 

9 

0 5 10 15 20 25 30 Min. 

Column: Alltech ® Allsep A-2 Anion, 100 x 4.6mm (Part No. 51219) 


with Lithium Hydroxide 



4 6 3 

5 

0 6 12 18 24 Min. 





anions 

1 

Chloride and Bromide (3500:1) 

Nickel Plating Bath Solution 

9172 9173 

2 

1. Chloride (3500ppm) 

2. Bromide (1ppm) 

1. Fluoride 

2. Unknown 

3. Chloride 

4. System 

5. Sulfate 

2 

3 

5 

1 

4 

0 2 4 6 8 10 12 Min. 





0 2 4 6 8 10 12 14 16 Min. 





Formate, Oxalate, Iodide, and Seven Common Anions 

3 4 

EDTA 

1 

9175 8284 

1 3 

1. Fluoride 

6. Nitrate 

1. Nitrite 

2. Formate 

7. Phosphate 

2 

2. EDTA 

3. Chloride 

8. Sulfate 

3. Nitrate 

4. Nitrite 

9. Oxalate 

4. Bromide 

4 

5. Bromide 

10. Iodide 

2 

5 6 8 

9 

7 

10 

0 2 4 6 8 10 12 14 16 Min. 

Column: Alltech ® Allsep A-2, 50 x 4.6mm (Part No. 51221) 

Mobile Phase: 2.8mM Sodium Bicarbonate:2.2mM Sodium Carbonate: 

2% Acetonitrile 



0 4 8 12 16 20 24 Min. 

Column: Alltech ® Anion/S, 100 x 4.6mm (Part No. 269012) 

Mobile Phase: 1.5mM Sulfuric Acid 






457


Anions 

3 

Anion Standards 

6159 

Haloacetic Acids 

13038 

2 

4 

1 

5 

7 

6 

8 

1. Fluoride 

2. Bicarbonate 

3. Chloride 

4. Nitrite 

5. Bromide 

6. Nitrate 

7. Phosphate 

8. Sulfate 

1 

2 

1. Chloro-difluoro Acetic Acid 

2. Difluoro Acetic Acid 

anions 

0 9 18 Min. 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min. 

Column: Alltech ® Anion/R, 10µm, 250 x 4.1mm (Part No. 269029) 


with Lithium Hydroixde 



Column: Alltech ® OA-1000, 9µm, 300 x 6.5mm (Part No. 9046) 

Mobile Phase: 1.0% Phosphoric Acid 



Detector: Conductivity (Non-Suppressed) 

1 


13037 

2 

3 



3. Malic Acid 




4 5 

6 

6 8 10 12 14 16 18 20 22 24 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Alltech ® Anion Exclusion, 10µm, 300 x 7.5mm 

(Part No. 269062) 

1.5mM Sulfuric Acid 

0.6mL/min 

Conductivity 



Cations 

Rapid Analysis of Monovalent 

and Divalent Cations 

13013 

Morpholine and Group I & II Cations 

8632 

2 

3 

4 

1 

5 

6 

1. Lithium 

2. Sodium 

3. Ammonium 

4. Potassium 

5. Magnesium 

6. Calcium 

2 3 4 5 6 

1 

7 

1. Lithium 

2. Sodium 

3. Ammonium 

4. Potassium 

5. Morpholine 

6. Magnesium 

7. Calcium 

0 1 

2 

3 

4 

5 

6 

7 8 9 Min. 

0 4 

8 12 16 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Alltech ® Universal Cation HR, 3µm, 53 x 7.0mm 

(Part No. 23109) 


2.5mL/min 

Conductivity 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Alltech ® Universal Cation, 7µm, 100 x 4.6mm 

(Part No. 27106) 


1.0mL/min 

Conductivity 

cations 

3 

2 

1 

5 

4 

6 

Rubidium and Barium 

7 

8 

1. Lithium 

2. Sodium 

3. Ammonium 

4. Potassium 

5. Rubidium 

6. Magnesium 

7. Calcium 

8. Barium 

6292 

1 

4 

2 

3 

5 

Ethanolamines 

1. Monoethanolamine 

2. Diethanolamine 

3. Triethanolamine 

4. Magnesium 

5. Calcium 

6315 

0 10 20 30 40 Min. 

0 10 20 30 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

1 


(Part No. 27106) 

5mM Hydrochloric Acid 

1.0mL/min 

Conductivity 

Trace Level Ammonium in Brine 

4 

8462 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 


(Part No. 27106) 

5mM Hydrochloric Acid 

1.0mL/min 

Conductivity 

Metal-Cyano Complexes 

5358 

3 

5 

1. Sodium 

2. Ammonium 

3. Potassium 

4. Magnesium 

5. Calcium 

1 

1. Fe(CN)6 4- 

2. Ag(CN)2 - 

3. Fe(CN)6 3- 

4. Ni(CN)4 2- 

5. Au(CN)2 - 

2 3 4 

5 

2 

0 5 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

10 15 20 Min. 


(Part No. 27106) 

2.5mM Oxalic Acid 

1.0mL/min 

Conductivity 

0 10 20 30 40 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 

Alltech ® Transition Metal C18, 7µm, 250 x 4.6mm 

(Part No. 269067) 

5mM Tetrabutylammonium Sulfate 

(Preformulated Alltech ® Low UV IPC A, Part No. 185149) 

in 30% Acetonitrile 

0.7mL/min 

UV at 214nm 




459


Cations 

1 

Cations in Coffee Extract 

Alkali Metals 

1 

6303 5204 

1. Potassium 

2. Magnesium 

3. Calcium 

2 

3 

1. Lithium 

2. Sodium 

3. Ammonium 

4. Potassium 

5. Cesium 

2 

4 5 

3 

cations 

0 13 26 Min. 

Column: Alltech ® Universal Cation, 7µm, 100 x 4.6mm 

(Part No. 27106) 

Mobile Phase: 5mM Hydrochloric Acid 



0 1 2 3 4 5 Min. 

Column: Hamilton ® PRP-X200, 10µm, 150 x 4.1mm 

(Part No. 79441) 

Mobile Phase: 4mM Nitric Acid in 30% Methanol 



12 

3 

4 

5 6 7 

8 

Metal Ions 

9 

1. Lithium 

2. Sodium 

3. Ammonium 

4. Potassium 

5. Nickel 

6. Zinc 

7. Cobalt 

8. Magnesium 

9. Calcium 

1 

2 34 

5 

6 

Transition Metals 

10318 13042 

7 

8 

1. Copper 

2. Sodium 

3. Ammonium 

4. Potassium 

5. Zinc 

6. Lead 

7. Magnesium 

8. Calcium 

0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 Min. 


(Part No. 27106) 




0 2 4 6 8 10 12 14 16 18 20 Min. 


(Part No. 27106) 




Cesium and Strontium 

13035 

12 3 

4 

5 

6 

7 

8 

1. Lithium 

2. Sodium 

3. Ammonium 

4. Potassium 

5. Cesium 

6. Magnesium 

7. Calcium 

8. Strontium 

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min. 

Column: 

Mobile Phase: 

Flow Rate: 

Detector: 


(Part No. 27106) 


1.0mL/min 

Conductivity 



Surfactants 

1 

2 

Short and Long Chain Cationic Surfactants 

3 

4 5 

6 

7 

2 

Toothpaste 

10317 8425 

1. Tetramethyl Ammonium 

1 

1. Unidentified 

2. Tetraethyl Ammonium 

2. Dodecyl Sulfate (C12) 

3. Tetrapropyl Ammonium 

4. Tetrabutyl Ammonium 

5. Dodecyltrimethyl Ammonium 

6. Cetyltrimethyl Ammonium 

7. Myristyltrimethyl Ammonium 

0 2 4 6 8 10 12 14 16 18 20 22 Min. 

0 2 

4 6 8 10 12 14 16 18 20 Min. 

Column: Alltech ® Surfactant/R Column, 7µm, 150 x 4.6mm 

(Part No. 25101) 

Mobile Phase: A: 2mM Nonafluoropentanoic Acid 



%B: 30 30 35 80 



Column: Alltech ® Surfactant/R, 7µm,150 x 4.6mm 

(Part No. 25101) 

Mobile Phase: A: 10mM Lithium Hydroxide 

B: Water:Acetonitrile:Methanol (20:60:20) 


%B: 3 3 20 50 



surfactants 

Alkyl Sulfates and Alkanesulfonates 

Alkyl Sulfates 

8428 

1 

5998 

1 2 

3 

4 

5 

6 

7 

8 

9 

1. C6 Sulfate 

2. C6 Sulfonate 



5. C8 Sulfate 


7. C10 Sulfate 


9. C12 Sulfate 

2 

3 

1. Octyl Sulfate 

2. Decyl Sulfate 

3. Dodecyl Sulfate 

0 4 

8 12 16 20 Min. 

Column: Alltech ® Surfactant/R, 150 x 4.6mm (Part No. 25101) 

Mobile Phase: A: 10mM Lithium Hydroxide 

B: Water:Acetonitrile:Methanol (20:60:20) 


%B: 3 3 20 50 



0 6 12 18 24 30 Min. 

Column: Alltech ® Surfactant C8, 5µm, 250 x 4.6mm 

(Part No. 269072) 

Mobile Phase: 0.02g/L Ammonium Acetate in 40% Methanol 






461

gc applications 

Abused Drugs 

2 

1 

3 

4 

Amphetamines 

1. Amphetamine 

2. Methamphetamine 

3. Propylamphetamine 

4. Ephedrine 

Club Drugs 

1013 1. Methamphetamine 

5. 2,3-MDMA 

2. 4-Methoxyamphetamine 

6. 3,4-MDMA 

2637 

3. 2,3-MDA 

7. 4-Bromo-2,5-dimethoxy- 

4. 3,4-MDA 

phenethylamine 

 

 

8. Ketamine 

9. Phencyclidine 

10. Cocaine 

11. Flunitazepam 

 

 

 

 

 

drugs 

0 1 2 3 4 Min. 

Column: Econo-Cap EC-1, 15m x 0.53mm x 1.20µm 

(Part No. 19650) 

Temp: 130°C 

Carrier Gas: Helium at 8.7mL/min 

Detector: FID 

1 

4 

Drug Mixture 

8 

0042 

1. Methylenedioxymethamphetamine 

(MDMA) 

2. Benzocaine 

3. Meperidine 

3 

2 

4. Phencyclidine (PCP) 

10 

5. Caffeine 

6. Procaine 

7 

13 


5 

8. Methadone 

6 

9. Atropine 

10. Cocaine 

9 

11 12 11. Oxazepam 

12. Codeine 

13. Diazepam 

 

 

Column: Heliflex ® AT-5ms, 15m x 0.25mm x 0.25μm 

(Part No. 15801) 

Temp: 




Drug Mixture 

1 

2 

3 

4 

5 

6 

7 

2483 

1. Caffeine 

2. Glutethimide 

3. Lidocaine 

4. Primary Methadone Metabolite 

5. Methadone 

6. Cocaine 


0 4 8 12 16 Min. 

Column: Heliflex ® Drug Two, 30m x 0.25mm x 0.25µm 

(Part No. 13746) 

Temp: 

230°C to 250°C at 5°C/min 


Detector: FID 

Horse Racing Drug Mixture 

0 

4 8 12 16 20 24 Min. 


(Part No. 19647) 

Temp: 210°C 


Detector: FID 

 

2597 

 

 

 

 

 

 



3. Caffeine 



6. Pentazocine 

7. Phenytoin 

8. Diazepam 

9. Nordiazepam 

10. Boldenone 

 

 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 15897) 

100°C (2 min hold) to 300°C at 15°C/min, then to 

320°C at 20°C/min 


FID 

462 



Ephedra Standards via MSTFA 

 

 

 

 

 

1. Methylephedrine 

2. Norpseudoephedrine 

3. Norephedrine 

4. Ephedrine 


6. Synephrine 

MDMA & MDA Analogs 

2663 2562 

1 

2 3 4 

1. 2,3-MDA 

2. 3,4-MDA 

3. 2,3-MDMA 

4. 3,4-MDMA 

 

 

0 

1 2 3 4 5 6 Min. 


(Part No. 15807) 

Temp: 





(Part No. 15801) 

Temp: 160°C 


Detector: FID 

Meperidine and Metabolite 

Nicotine and Metabolite 

2 

1027 

1 

1030 

1 

1. Meperidine 

2. Normeperidine 

2 

1. Nicotine 

2. Cotinine 

drugs 

0 1234 567 Min. 


(Part No. 19647) 

Temp: 240°C 


Detector: FID 

0 2 4 6 8 10 Min. 


(Part No. 19652) 

Temp: 180°C 


Detector: FID 

Drug Screening 

 

1. Methoxyflurane 

2. Trimethadione 



 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

5. α,α-Dimethyl-β-methylsuccinimide 


7. Propylamphetamine 

 

 

 

 

 

 

 

 

 

 

8. 1,3-Dimethylbarbituric Acid 

9. Nicotine 

10. Ethinamate 


12. Phendimetrazine 

13. Barbital 

14. Methyprylon 




18. Phenacetin 



Column: 

Temp: 

Carrier Gas: 

Detector: 

21. Meperidine 


23. Ethotoin 

24. Benzphetamine 

25. Hexobarbital 

26. Lidocaine 


28. Doxylamine 



31. Brompheniramine 

32. Cocaine 

33. Imipramine 

Econo-Cap EC-5, 30m x 0.25mm x 0.25µm 

(Part No. 19647) 

100°C to 300°C at 10°C/min 


FID 

1841 




463


Abused Drugs 

2 

1 

3 

Opiates 

1. 6-Acetylcodeine 

2. 6-Monoacetylmorphine 

3. Diacetylmorphine (Heroin) 

Stimulant Drug Mixture 

1026 

 

 

2581 


 


3. Methylphenidate 

4. Caffeine 

5. Cocaine 

 

0 

4 8 12 16 Min. 


(Part No. 19647) 

Temp: 260°C 


Detector: FID 

 


(Part No. 15897) 

Temp: 



Detector: FID 

Stimulants 

Underivatized Basic Drugs 

drugs 

1 

2 

3 

4 

5 



3. Methylphenidate 

4. Caffeine 

5. Cocaine 

1031 



3. Nicotine 

2 3 

1 

4 

5 

6 

7 

8 

4. Ephedrine 

5. Benzocaine 

6. Benzphetamine 

1011 

12 

9 

13 

1415 

18 

16 

17 

7. Phencyclidine 

8. Doxylamine 

9. Procaine 

10. Methadone 

11. Imipramine 

12. Cocaine 


14. Scopolamine 

15. Codeine 

16. Norcodeine 

17. Morphine 

18. Heroin 

1870 

0 

4 8 12 16 20 Min. 

0 2 4 6 8 10 12 14 16 18 20 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 19652) 



FID 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 13857) 



FID 

Nutraceuticals 

Sterols in Saw Palmetto 

1. Campesterol 

2. Stigmasterol 

3. Sitosterol 

1 2 

3 

2580 

1 

2 

3 

Kava-Kava Lactones 

4 

5 

6 

1. Dihydrokavain 

2. Kavain 

3. Desmethoxyyangonin 

4. Dihydromethysticin 

5. Yangonin 

6. Methysticin 

2559 

0 3 6 9 12 15 18 21 24 27 Min. 0 2 4 6 8 10 12 Min. 



(Part No. 15891) 

(Part No. 15807) 

Temp: 


Temp: 

250°C to 280°C at 5°C/min 




Detector: FID 

464 



Organic Volatile Impurities 

12 

3 

4 

5 

6 

7 

USP 467 — Trace Volatiles 

8 


2. Impurity 


4. Chloroform 

5. Benzene 



8. Dimethyl Sulfoxide 

1797 

1 

2 

3 

4 

5 6 

USP 467 — Method V 



3. Chloroform 

4. Benzene 



1866 

0 3 6 9 12 15 18 Min. 


(Part No. 16859) 

Temp: 




0 4 8 12 16 20 Min. 


(Part No. 16889) 

Temp: 40°C 


Detector: FID 

Pharmaceutical Solvents (Class 2) Composite Mix 

0 

1 2 

Column: 

Temp: 

Carrier: 

Detector: 

3 

4 

5 

6 

7 

8 

9 

10 

11 

13 

12 

17 

18 

14 

15 

16 

19 

20 

21 

22 

24 23 

25,26 

5 10 15 20 25 30 Min. 


(Part No. 15809) 

40°C (8 min hold) to 105°C at 5°C/min, then to 225°C at 20°C/min 

Helium at 1.05mL/min (27cm/sec) 

FID 

27 

28 

29 

30 

1. Methanol 

2. Acetonitrile 

3. Dichloromethane 

4. Nitromethane 

5. trans-1,2-Dichoroethene 

6. Hexane 

7. cis-1,2-Dichloroethene 

8. Chloroform 

9. 2-Methoxyethanol 

10. 1,2-Dimethoxyethane 

11. Cyclohexane 

12. Formamide 

13. Ethylene Glycol 

14. 1,1,2-Trichloroethene 


16. 2-Ethoxyethanol 

17. Methylcyclohexane 

18. Pyridine 

19. Toluene 

20. N,N-Dimethylformamide 

21. Methylbutyl Ketone 


23. N,N-Dimethylacetamide 


25. m-Xylene 

26. p-Xylene 

27. o-Xylene 

28. N-Methylpyrrolidone 

29. Tetralin 

30. Sulfolane 

2566 

drugs 

Blood Alcohols 

Blood Alcohol Test Mix 

1 

3 

1016 

Pharmaceuticals 

Sedatives 

1033 

2 

4 

5 

1. Acetaldehyde 

2. Acetone 

3. Methanol 

4. 2-Propanol 

5. Ethanol 

 

 

1. Alprazolam 

2. Triazolam 

0 1 2 3 4 5 Min. 

Column: Econo-Cap EC-Wax , 30m x 0.32mm x 0.25µm 

(Part No. 19654) 

Temp: 50°C 


Detector: FID 

 


(Part No. 19647) 

Temp: 280°C 


Detector: FID 




465


Pharmaceuticals 

1 2 

Depressants 

3 

4 

5 

1. Glutethimide 

2. Methaqualone 

3. Diazepam 

4. Chlorpromazine 

5. Flurazepam 

2560 

1 

2 

3 

4 


5 6 



3. Normethsuximide 


5. PEMA 

6. MPEMA 

2568 

0 

2 4 6 8 10 12 14 Min. 


(Part No. 15807) 

Temp: 270°C 


Detector: FID 

0 1 2 3 4 5 6 Min. 


(Part No. 15801) 

Temp: 



Detector: FID 



2187 

4 

1005 

drugs 

1 

3 

4 

2 

6 

5 

7 

8 



3. Doxepin (cis-Doxepin) 

4. Imipramine 

5. Doxepin (trans-Doxepin) 


7. Maprotyline 

8. Clomipramine 

1 

2 

3 

5 

6 

7 


2. Doxylamine 





7. Promethazine 

0 

4 8 12 16 20 Min. 0 2 4 6 8 Min. 

Column: Econo-Cap AT-20, 30m x 0.32mm x 0.25µm 

(Part No. 19677) 

Temp: 235°C 

Carrier Gas: Helium at 22 cm/sec 

Detector: FID 



(Part No. 19650) 

Temp: 210°C 


Detector: FID 

Hypnotics 

2567 

4 

1009 

1 

3 

2 

4 5 6 


2. Doxylamine 





2 

3 

5 

1. Trichloroethanol 

2. Allobarbital 




1 

0 

1 2 3 4 5 Min. 

0 2 4 6 8 10 12 14 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 15801) 



FID 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 19651) 

160°C to 220°C at 10°C/min 


FID 

466 



Fast GC Drug Analysis 

Fluoxetine and Metabolite 

 

 

 

 


2. α-Methyl-α-propylsuccinimide 


4. Barbital 


6. Phensuximide 

 

 

7. N-Normethsuximide 


9. Ethotoin 


11. 2-Ethyl-2-phenylmalonamide 


13. Methyl PEMA 

2262 1786 

 

1. Norfluoxetine 

2. Fluoxetine 

 

 


(Part No. 14454) 

Temp: 240°C 


Detector: FID 

 


(Part No. 13640) 

Temp: 200°C 


Detector: FID 

Sedatives 

1 

2 

3 

9 

10 

11 

6 

4 

5 7 8 

12 

13 

14 

15 

1. Ethchlorvynol 

2. Ethinamate 

3. Methyprylon 


5. Butalbital 



8. Meprobamate 

9. Prazepam 

10. Hexobarbital 

11. Alphenal 


13. Halazepam 

14. Chlordiazepoxide 

15. Triazolam 

1003 

drugs 

0 2 4 6 8 10 12 14 16 18 20 22 24 26 Min. 

Nitrazepam and ANB Metabolite 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 19651) 

160°C to 220°C at 10°C/min 


FID 

 

1. Nitrazepam 

2. 2-Amino-5-nitrobenzophenone 

2592 

 

 

 


(Part No. 15897) 

Temp: 280°C 


Detector: FID 




467


Steroids 

1 

2 3 

4 

5 

6 

7 

Steroids, TMS Derivatives 

11 

10 

12 

0 2 4 6 8 10 12 14 Min. 

8 

9 

1. Androsterone 

2. Estrone 

3. Estradiol 



6. 5-α-Cholestane 

(underivatized) 

7. Estriol 

8. Coprostanol 

9. Cholesterol 

10. Campesterol 

11. Stigmasterol 

12. β-Sitosterol 


(Part No. 19651) 

Temp: 290°C 


Detector: FID 

1008 

1 

2 3 

4 

5 

Anabolic Steroids 

1. Nandrolone 


3. Norethandrolone 

4. Clostebol 

5. Fluoxymesterone 

0 2 4 6 8 10 Min. 


(Part No. 19647) 

Temp: 300°C 


Detector: FID 

1019 

environmental 

Chlorinated Disinfection 

EPA Method 551: Chlorinated Disinfection 

 

Byproducts by NPD 

2280 

 

1. Trichloroacetonitrile 

2. Dichloroacetonitrile 

3. Bromochloroacetonitrile 

4. Dibromoacetonitrile 

 

 


(Part No. 13658) 

Temp: 



Detector: NPD 

Haloacetic Acids 

EPA Method 552.1: Haloacetic Acids 

1. Monochloroacetic Acid 

2. Monobromoacetic Acid 

3. Dalapon 

4. Dichloroacetic Acid 

5. Trichloroacetic Acid 

6. Bromochloroacetic Acid 

7. Dibromoacetic Acid 

0 

Column: 

Temp: 

Carrier Gas: 

Detector: 

1 

4 

2 

3 

2 4 6 8 10 12 14 16 18 Min. 


(Part No. 13690) 



ECD 

5 

6 

7 

2105 

Pesticides 

1 2 3 

4 

Carbamates 

1. Naled 

2. Metacil 

3. Zectran 

4. Carbaryl 

1232 

Organophosphorus Pesticides 

1. TEPP 

14. Carbophenothion 

2. Sulfotepp 

15. Phosmet 

3. Monocrotophos 16. EPN 

4. Dimethoate 17. Leptophos 

5. Dioxathion 

18. Azinphos-ethyl 

6. Terbufos 

2 

7. Phosphamidon 

4 

8. Malathion 

6 

9. Parathion 

10. cis-Chlorfenvinphos 

3 

11. trans-Chlorfenvinphos 

12. Ethion 

1 

13. Famphur 

5 

7 

8 9 

10 

11 

16 

12 

14 

13 15 17 18 

2469 

0 5 Min. 

Column: Heliflex ® AT-Pesticide, 20m x 0.53mm x 0.60µm 

(Part No. 16846) 

Temp: 200°C 


Detector: ECD 

Column: 

Temp: 

Carrier Gas: 

Detector: 

4 8 12 16 20 24 28 32 36 40 Min. 


(Part No. 15807) 



MSD 

468 



 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Chlorinated and Organophosphorus Pesticides 

 

 

 

1. TEPP 

2. Monocrotophos 

3. α-BHC 

4. Sulfotep 

5. Dimethoate 

6. β-BHC 

7. γ-BHC 

8. δ-BHC 

9. Heptachlor 

10. Aldrin 

11. Malathion 

12. Parathion 

Column: 

Temp: 

Carrier Gas: 

Detector: 



15. Dieldrin 

16. p,p'-DDE 

17. Endrin 


19. p,p'-DDD 



22. p,p'-DDT 

23. EPN 



(Part No. 13658) 



NPD 

2315 


2466 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1. Etridiazole 

2. Chloroneb 

3. Propachlor 

4. Trifluralin 

5. α-BHC 

6. β-BHC 

7. Pentachloronitro-benzene 

8. γ-BHC 

9. Diclone 

10. Chlorothalonil 

11. δ-BHC 

12. Alachlor 

13. Heptachlor 

14. Aldrin 

15. Dacathal 


17. γ-Chlordane 

18. α-Chlordane 


20. trans-Nonachlor 

21. p,p’-DDE 

22. Dieldrin 

23. Perthane 

24. Nitrofen 

25. Endrin 

26. Chloropropylate 


28. p,p’-DDD 


30. p,p’-DDT 


32. Captafol 

33. Endrin Ketone 


35. Kelthane 

36. Mirex 

37. cis-Permethrin 


(Part No. 15807) 

Temp: 

80°C (1 min hold) to 100°C at 15°C/min, to 160°C at 

10°C/min, to 285°C at 5°C/min 


Detector: MSD at 300°C 

environmental 

 

Pesticide Mix 

 


 

 

 

 

 

1. Diazinon 

2. Dimethoate 

3. Lorsban 

4. Malathion 

5. Parathion 

6. Ethion 

1853 

 

 

 

 

 

 

 

 

 

 

 

 

1. α-BHC 

2. γ-BHC 

3. β-BHC 

4. δ-BHC 

1569 

5. Heptachlor 

6. Aldrin 



9. p,p'-DDE 

10. Dieldrin 

11. Endrin 

12. p,p’-DDD 


14. p,p’-DDT 



 

 

 


(Part No. 13969) 

Temp: 200°C 

Carrier Gas: Helium at 5mL/min (37.3cm/sec) 

Detector: ECD 

 


(Part No. 13662) 

Temp: 

190°C to 240°C at 3°C/min 


Detector: ECD 




469


Pesticides 

EPA Method 508 — Chlorinated Pesticides 

EPA Method 505 — Organohalide Pesticides 

(Chlordane) 1552 7 10 

8 11 

1612 

9 1. Hexachlorocyclopentadiene 

12 

2. Simazine 

3. Atrazine 

1 

5 

4 

2 3 6 

13 

14 

4. Hexachlorobenzene 

5. Lindane 

6. Alachlor 

7. Heptachlor 

8. Aldrin 




12. Dieldrin 

13. Endrin 


 

0 4 8 12 16 20 24 28 32 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 19646) 

60°C to 300°C at 4°C/min 


ECD 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 19660) 

180°C to 260°C at 4°C/min 


ECD 

EPA Method 507, Nitrogen and Phosphorus Containing Pesticides by GC/NPD 

environmental 

1 

2 

4 

3 

5 

6 

7 

8 9 16 

15 

11 10 

17 

12 

0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 Min. 

14 

13 18 

23 

19 

20 22 

21 24 

25 

1. Dichlorvos 

2. EPTC 

3. Vernolate 

4. Pebulate 

5. Tebuthiuron 

6. Molinate 

7. Ethoprop 

8. Atraton 

9. Simazine 

10. Atrazine 

11. Propazine 

12. Terbufos 

Column: 

Temp: 

Carrier Gas: 

Detector: 

13. Pronamid 

14. Disulfoton 

15. Alachlor 

16. Ametryn 

17. Terbutryn 

18. Metolachlor 

19. MGK 264 (Peak 1) 

20. MGK 264 (Peak 2) 

21. Merphos (Peak 1) 

22. Butylate 

23. Napropamide 

24. Merphos (Peak 2) 

25. Fenarimol 

1551 


(Part No. 19646) 



NPD 

EPA Method 508 — Chlorinated Pesticides in Water 

1 

2 

3 

4 

5 

0 4 8 12 16 20 24 28 32 36 40 44 48 52 Min. 

6 

7 

8 

12 14 

9 

15 

11 13 16 

10 

20 

17 

18 

19 

21 

22 

23 

1. Propachlor 

2. Trifluralin 

3. α-HCH 


5. β-HCH 

6. γ-HCH 

7. δ-HCH 

8. Heptachlor 

9. Aldrin 

10. DCPA 



Column: 

Temp: 

Carrier Gas: 

Detector: 


14. Dieldrin 

15. p,p'-DDE 

16. Endrin 

17. p,p'-DDD 



20. p,p'-DDT 


22. cis-Permethrin 

23. trans-Permethrin 

1550 


(Part No. 19646) 



ECD 

470 



 

Semivolatiles 

Column: 

 

 

 

 

 

 

 

 

Temp: 

Carrier Gas: 

Detector: 

1. Phenol 





Phenols 

6. 4-Chloro-3-Methylphenol 




10. 2-Methyl-4,6-Dinitrophenol 


 


(Part No. 13656) 



FID 

Phthalate and Adipate Esters 

2286 

 

 

 

 




4. Hexachloroethane 

 

 

 

Base/Neutral Mix 

 

5. Nitrobenzene 

2207 

6. Isophorone 

7. 1,2,4-Trichlorobenzene 

8. Hexachlorobutadiene 

9. Hexachlorocyclopentadiene 

10. 2-Chloronaphthalene 



13. Azobenzene 


 


(Part No. 13658) 

Temp: 



Detector: FID 

EPA Method 606 - Phthalates 

0 

1 

2 

3 

4 

5 

6 7 

5 10 15 20 25 30 35 40 45 Min. 

2104 

1. Dimethyl Phthalate 

2. Diethyl Phthalate 

3. Di-n-Butyl Phthalate 

4. Butylbenzyl Phthalate 

5. Di(2-ethylhexyl) Adipate 

6. Di(2-ethylhexyl) Phthalate 

7. Di-n-Octyl Phthalate 


(Part No. 13640) 

Temp: 




0 

Column: 

1 

2 

3 

4 8 12 16 20 24 28 Min. 

Temp: 

Carrier Gas: 

Detector: 

4 

5 

6 

1. Dimethylphthalate (20ng) 

2. Diethylphthalate (20ng) 

3. Di-n-butylphthalate (20ng) 

4. Butylbenzyl Phthalate (20ng) 

5. Bis(2-ethylhexyl)phthalate (20ng) 

6. Di-n-octylphthalate (20ng) 


(Part No. 13569) 



FID 

2583 

environmental 

EPA SW846 Method 8091 

Nitroaromatics and Cyclic Ketones 

EPA Method 612: Chlorinated Hydrocarbons 

2114 

1840 

1. Isophorone 


1 2 2. Nitrobenzene 

5. 1,3-Dinitrobenzene 

3. 1,4-Napthoquinone 6. 2,4-Dinitrotoluene 

3 4 6 5 

1 23 4 

5 

6 

7 

8 

9 




4. Hexachloroethane 



7. Hexachlorocyclopentadiene 



0 4 8 12 16 20 Min. 


(Part No. 13795) 

Temp: 


Carrier Gas: Helium at 44.5cm/sec 

Detector: FID 

0 2 4 6 8 10 12 14 16 18 Min. 


(Part No. 13970) 

Temp: 



Detector: FID 




471


Semivolatiles 

EPA Method 610: Polynuclear Aromatic Hydrocarbons 

Fast PAH Analysis 

 

 




4. Fluorene 

 

 

 

 

 

 

 

 

5. Phenanthrene 2208 

6. Anthracene 


8. Pyrene 


10. Chrysene 




14. Indeno[1,2,3-c,d]pyrene 



 

 




4. Fluorene 


6. Anthracene 

 

 

 

 

 

 


2284 

8. Pyrene 


10. Chrysene 







 



(Part No. 13658) 

(Part No. 14438) 

Temp: 


Temp: 

100°C to 340°C at 20°C/min 


Carrier Gas: Helium at 0.1mL/min (25 cm/sec) 

Detector: FID 

Detector: FID 

environmental 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

PAHs 

Column: 

Temp: 

Carrier Gas: 

Detector: 




4. Fluorene 


6. Anthracene 


8. Pyrene 


10. Chrysene 








(Part No. 15807) 

80°C (1 min hold) to 160°C at 25°C/min, 

to 300°C at 3°C min, 

to 325°C at 25°C/min 


FID 

2463 

Phenols Mix on AT-1ms 

2621 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1. Phenol 


3. o-Cresol 

4. m & p-Cresol 





9. 4-Chloro-3-methylphenol 

10. 2,4,6-trichlorophenol 




14. 2,3,4,6-Tetrachlorophenol 

15. 2-Methyl-4,6-dinitrophenol 



(Part No. 15897) 

Temp: 

60°C (0.5 min hold) to 235°C at 10°C/min 

Carrier: 

Helium at 1mL/min (28cm/sec) 


472 



Volatiles 

5,6 

Aromatic Solvents 

1 

2 

4 

3 

7 

8 

9 

10 

11 

1. Benzene 

2. Toluene 



5. m-Xylene 

6. p-Xylene 

7. o-Xylene 




11. Napthalene 


2588 

12 

0 

2 4 6 8 10 12 14 16 18 20 22 24 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 13659) 



FID 

1 7 

OSHA Method 07: Organic Vapors 

2589 

0 

3 

2 

5 

6 

4 

8 

9 

11,12 

10 

13 

14 

15 

2 4 6 8 10 12 14 16 18 20 22 Min. 

16 

17 

18 

19 

20 

Column: 

Temp: 

Carrier Gas: 

Detector: 

1. Pentane 

2. Hexane 

3. Isopropyl Ether 

4. Isooctane 

5. Heptane 


7. Carbon Disulfide 


9. trans-1,2-Dichloroethene 

10. Ethyl Acetate 

11. tert-Butyl Alcohol 

12. Isopropyl Acetate 

13. Propyl Acetate 


15. Isobutyl Acetate 


17. Butyl Acetate 

18. 2-Butanol 

19. Cumene 


Heliflex ® AT-Wax, 30m x 0.32mm x 1.00µm 

(Part No. 137382) 



FID 

environmental 

1 3 

2 

7 

5 

6 9 

8 

10,11 

16 

12 13 

15 

14 

17 

18 

19 

OSHA Method 07: Organic Vapors 

20 

1. Pentane 

2. tert-Butyl Alcohol 


4. trans-1,2-Dichloroethene 

5. Hexane 

6. Isopropyl Ether 



9. 2-Butanol 


11. Isopropyl Acetate 

12. Isooctane 

13. Heptane 







20. Cumene 

2590 

0 

4 

2 4 6 8 10 12 14 16 18 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 13659) 



FID 




473


Volatiles 

4 

5 

3 

12 

6 

7 

EPA Method 601 — 

EPA Method 602 — 

1 7 

Purgeable Halocarbons 1132 2 3 4 

5 Purgeable Aromatics 

1138 

1. Methyl Chloride 


3. Dichlorofluoromethane 

4. Methyl Bromide 

5. Ethyl Chloride 

6. Trichlorofluoromethane 


6 

1. Benzene 

2. Toluene 






0 2 4 6 8 10 Min. 


(Part No. 16843) 

Temp: 35°C 


Detector: FID 

0 3 6 9 12 15 Min. 

Column: Heliflex ® AT-WAX, 15m x 0.53mm x 5.00µm 

(Part No. 16883) 

Temp: 



Detector: FID 

Halocarbons 

8 

1133 

environmental 

1. 1,1-Dichloroethane 

2. Chloroform 


4. 1,1,1-Trichloroethane 


6. 1,2-Dichloropropane 

7. Bromodichloromethane 

+ Trichloroethene 

8. 2-Chloroethyl Vinyl Ether 

9. trans-1,3-Dichloropropene 

10. cis-1,3-Dichloropropene 

Column: 

Temp: 

Carrier Gas: 

Detector: 


12. Dibromochloromethane 



15. Bromoform 

16. 1,1,2,2-Tetrachloroethane 

17. m-Dichlorobenzene 

18. p-Dichlorobenzene 

19. o-Dichlorobenzene 


(Part No. 16843) 

40°C (5 min hold) to 200°C/min at 5°C/min 


FID 

1 

2 

3 4 

5 

6 7 9 

11 

10 

0 5 10 15 20 25 30 Min. 

12 

13 

14 

15 

16 

17 19 18 

12 

18 

Priority Pollutant Analysis 

1234 

21 23 

9 

13 

17 

22 

15 

11 

10 12 16 

14 

5 7 

20 

3 

19 

4 6 

8 

0 4 8 12 16 20 24 28 Min. 

1. Methane 

2. Chloromethane 

3. Chloroethane 


5. Bromochloromethane 

6. Chloroform 







Column: 

Temp: 

Carrier Gas: 

Detector: 





17. Chlorodibromomethane 


19. Bromoform 






(Part No. 16889) 



FID 

474 



EPA Method 624 — Purge & Trap Volatiles 

1660 

1. Chloromethane 


3. Bromomethane 

4. Chloroethane 

5. Trichlorofluoromethane 



8. trans-1,2-Dichloroethane 


10. Chloroform 



13. Benzene 






19. Toluene 


21. Tetrachloroethene + 1,1,2-Trichloroethane 

22. Dibromochloromethane 



25. Bromoform 





 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Column: Heliflex ® AT-624, 60m x 0.53mm x 3.00µm, (Part No. 13800) 

Temp: 



Sample: 200ppb in 5mL H20 

Trap: 

Purge 11 min at 35mL/min, Desorb at 275°C for 4 min 

Detector: MSD 

 

 

 

environmental 

NIOSH 1450 — Acetates 

TCLP Volatiles Mix 

2 

1 

3 4 56 

7 

8 9 10 


2. tert-Butyl acetate 


4. sec-Butyl acetate 

5. Ethyl Acrylate 



8. Isoamyl Acetate 

9. Amyl Acetate 

10. 2-Ethoxyethyl Acetate 

2591 

1 

2 

3 

4 

5 

6 

7 

8 

9 

10 


2. 1,1-Dichloroethene 

3. 2-Butanone 

4. Chloroform 


6. Benzene + 1,2-Dichloroethene 




10. 1, 4-Dichlorobenzene 

2064 

0 

2 4 6 8 10 12 14 16 18 Min. 

0 

3 6 9 12 15 18 21 24 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 137382) 



FID 

Column: 

Temp: 

Carrier Gas: 

Detector: 

Heliflex ® AT-502.2, 30m x 0.53mm x 3.00µm 

(Part No. 13792) 



FID 




475


Volatiles 

EPA Method 602 — Purgeable Aromatics 

 

 

 

 

 

 

1233 

1. Benzene 

2. Toluene 






UST 

 

 

1. n-Octane 

2. n-Decane 

3. n-Dodecane 


TRPH Standard (Florida) 

5. n-Hexadecane 

6. n-Octadecane 

7. n-Eicosane 

8. n-Docosane 

 

 

 

 

2622 

9. n-Tetracosane 

10. n-Hexacosane 

11. n-Octacosane 

12. n-Triacontane 

13. n-Dotriacontane 

14. n-Tetratriacontane 

15. n-Hexatriacontane 

16. n-Octatriacontane 

17. n-Tetracontane 

 


(Part No. 13756) 

Temp: 



Detector: FID 

 

Column: Heliflex ® AT-1ht, 15m x 0.25mm x 0.10μm 

(Part No. 16367) 

Temp: 


Carrier: 

Helium at 1mL/min (33cm/sec) 



1 

2 

3 


4 5 

6 







3 

4 5 Short Chain Free Fatty Acids 

2528 1808 

2 


1 6 

7 


3. Isobutyric Acid 

8 

4. n-Butyric Acid 

5. Isovaleric Acid 

6. n-Valeric Acid 



foods, flavors, & fragrances 

0 

 

 

 

2 4 6 8 10 Min. 

Column: Heliflex ® AT-AquaWax-DA, 30m x 0.25mm x 0.25µm 

(Part No. 14537) 

Temp: 180°C 


Detector: FID 

 

C1-C3 Acids (Aqueous) 

1. Water 




1072 

0 3 6 9 Min. 


(Part No. 19684) 

Temp: 150°C 


Detector: FID 

1 2 

3 

4 

5 

Low Molecular Weight Acids 

6 

7 

8 




4. Isobutyric Acid 


6. Isovaleric Acid 



1801 

 

Column: 6ft x 2mm i.d., Glass 

Packing: Porapak ® QS, 80/100 (Part No. 2719) 

Temp: 175°C 


Detector: TCD 

0 4 8 12 16 20 Min. 

Column: 2m x 2mm i.d., Glass 

Packing: 4% Carbowax ® 20M on Carbograph 1-DA, 

80/120 (Part No. 17271) 

Temp: 180°C 

Carrier Gas: Nitrogen at 20mL/min 

Detector: FID 

476 



Alcohols 

The Glenlivet ® Scotch 

2525 

3 

6,7 

Red Wine Spiked with Off Flavor 

2539 

2 

1 

4 

5 

8 9 


2. Methanol 

3. Ethanol 

4. 1-Propanol 

5. Isobutanol 

6. 2-Methyl-1-butanol 


8. 4-Ethylguaiacol 

9. 4-Ethylphenol 

0 5 10 15 20 25 30 35 40 Min. 

Column: Heliflex ® AT-AquaWax, 60m x 0.25mm x 0.25µm 

(Part No. 12447) 

Temp: 


200°C at 5°C/min 


Detector: FID 

 

 

 

Column: 

 

 

 

 

Temp: 

Carrier Gas: 

Detector: 

 

 

 

 

 

Scotch Whiskey 

 

 

 



3. Methanol 

4. Ethanol 

5. n-Propanol 

6. Isobutanol 

7. Amyl Alcohol 

8. Isoamyl Alcohol 


Econo-Cap EC-Wax, 15m x 0.53mm x 1.20µm 

(Part No. 19653) 

40°C (3 min hold) to 150°C at 25°C.min 

Helium at 3mL/minute 

FID 

Scotch Whiskey 


2. Methanol 

3. Ethanol 


5. n-Propanol 

6. Isobutanol 

7. Pentanol 

8. Isopentanol 


2035 

1818 

0 

1 

2 

3 

5 10 15 20 25 30 35 40 45 50 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 

4 

5 7 8 9 

6 

10 

11 

12 

13 

Heliflex ® AT-AquaWax-DA, 60m x 0.25mm x 0.25µm 

(Part No. 14547) 



FID 

1. Methanol 

2. Ethanol 

3. 2-Propanol 

4. 1-Propanol 

5. 2-Methyl-2-propanol 

6. 2-Butanol 


14 15 

16 

0 2 4 6 8 10 Min. 

C1-C5 Alcohols 

8. 1-Butanol 


10. 2,2-Dimethyl-1-propanol 


12. 3-Pentanol 





Column: 6ft x 2mm i.d., Glass, 0.2% Carbowax ® 1500 

Packing: Carbograph 2, 60/80 (Part No. 1725) 

Temp: 130°C 


Detector: FID 

Irish Whiskey 

1815 

2524 


 


Packing: Carbograph 1 AW 20, 80/120 

Temp: 



Detector: FID 

 


(Part No. 12447) 

Temp: 


200°C at 5°C/min 






477


Amino Acids 

N(O,S)-Heptafluorobutyl-isobutyl Esters of Amino Acids from Soy Protein Hydrolysate 

1241 

 

 

 

 

 

 

 

 

 

 

 

 

 

1. Alanine 

2. Glycine 

3. Valine 

4. Threonine 

5. Serine 

6. Leucine 

7. Isoleucine 

8. Proline 

9. Cystine 





14. Lysine 

15. Tyrosine 

16. Arginine 

17. Histidine 

18. Tryptophan 

 

 

 

 

 

 

Column: 

Temp: 

Carrier Gas: 

Detector: 

Heliflex ® AT-Amino Acid, 25m x 0.53mm x 1.20µm 

(Part No. 16864) 



FID 


Antioxidants 

0 1 

Column: 

Temp: 

Carrier Gas: 

Detector: 

Phenolic Antioxidants 

1 

3 

2 

4 

1. BHA 

2. BHT 

3. TBHQ 

4. 3,5-Di-tert-butyl-4- 

Hydroxybenzyl Alcohol 

5. THBP 

6. Propyl Gallate 

2 3 4 5 6 Min. 


(Part No. 13656) 



FID 

5 

6 

2264 

Essential Oils 

2 

1 

3 

4 

56 

7 

8 

10 

9 11 

12 

13 

14 

0 5 10 15 20 25 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 

Aroma Test Mix 

1. trans-2-Hexenal 

2. p-Cymene 

3. cis-3-Hexenol 

4. trans-2-Hexenol 

5. Ethyl Octanoate 

6. 1-Octene-3-ol 

7. Benzaldehyde 


(Part No. 972510) 



FID 

1304 

8. Pentadecane 

9. Linalool 


11. Carvone 

12. Hexadecane 

13. Methyl Salicylate 


478 



Essential Oils 

1 2 3 4 

5 

Citrus Reference Mix Peppermint Oil 

1311 2529 

6 

7 8 

9 

10 

1. α-Pinene 

2. Camphene 

3. β-Pinene 

4. p-Cymene 

5. Limonene 

6. Nonanal + Linalool 

7. Terpen-4-ol 

8. γ-Terpineol 

9. Neryl Acetate 

10. Geranyl Acetate 

0 2 4 6 8 10 Min. 


(Part No. 935110) 

Temp: 



Detector: FID 

0 5 10 15 20 25 30 35 40 Min. 


(Part No. 12437) 

Temp: 



Detector: FID 

 

FAMEs 

1 

2 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

4 5 

3 

C14:0-C24:0 Fatty Acid Methyl Esters 

6 

7 

9 

8 

10 

1317 

1. Methyl Myristate (C14:0) 

2. Methyl Palmitate (C16:0) 

3. Methyl Stearate (C18:0) 

4. Methyl Oleate (C18:1 cis) 

5. Methyl Linoleate (C18:2 cis,cis) 

6. Methyl Linolenate (C18:3 cis,cis,cis) 

7. Methyl Arachidate (C20:0) 

8. Methyl Behenate (C22:0) 

9. Methyl Erucate (C22:1 cis) 

10. Methyl Lignocerate (C24:0) 

Lemon Oil 

Column: 

Temp: 

Carrier Gas: 

Detector: 

 

1. α-Thujene 

2. α-Pinene 

3. Camphene 

4. Sabinene 

5. β-Pinene 

6. 6-Methyl-5-hepten-2-one 

7. Myrcene 

8. Octanol 

9. α-Phellandrene 

10. 3-Carene 

11. α-Terpinene 

12. p-Cymene 

13. Limonene 

14. γ-Terpinene 

 

 

 

 

 

2288 

15. Terpindene 

16. Linalool 

17. Nonanal 

18. Citronellal 

19. Terpinen-4-ol 

20. α-Terpineol 

21. Neral 

22. Gerinal 

23. Neryl Acetate 

24. Geranyl Acetate 

25. β-Caryophyllene 

26. trans-α-Bergamotene 

27. β-Bisabolene 


(Part No. 13656) 



FID 

Canola Oil 

2333 

1. Methyl Myristate (C14:0) 

2. Methyl Palmitate (C16:0) 

3. Methyl Palmitoleate (C16:1 cis) 

4. Methyl Stearate (C18:0) 

5. Methyl Oleate (C18:1 cis) 

6. Methyl Linoleate (C18:2 cis,cis) 

7. Methyl Linolenate 

(C18:3 cis,cis,cis) 

8. Methyl Arachidate (C20:0) 


0 4 8 12 16 Min. 

Column: Heliflex ® AT-Wax, 25m x 0.25mm x 0.20µm 

Column: Econo-Cap EC-Wax, 30m x 0.25mm x 0.25µm 

(Part No. 9925252) 

(Part No. 19655) 

Temp: 

180°C to 240°C at 5°C/min 

Temp: 


Carrier Gas: Hydrogen at 0.8mL/min 


Detector: FID 

Detector: FID 




479


FAMEs 

2 

3 

5 

6 

7 

8 

13 

9 12 

10 11 

15 

16 

17 

PUFA (Animal Source) 

1. C14:0 

2. C16:0 

3. C16:1 ω7 

4. C18:0 

5. C18:1 ω9 

6. C18:1 ω7 

7. C18:2 ω6 

8. C18:3 ω3 

9. C20:1 ω11 

10. C20:1 ω9 

11. C20:2 ω6 

12. C20:3 ω6 

13. C20:4 ω6 

14. C20:5 ω3 

15. C22:4 ω6 

16. C22:5 ω3 

17. C22:6 ω3 

1845 

1 

14 

4 

0 5 10 15 20 25 30 35 40 45 50 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 136462) 

160°C to 250°C at 2°C/min 


FID 


1 3 5 6 8 12 19 20 

 

 

2 

4 

7 

23 

9 1011 

18 

17 

22 

16 

14 

13 21 

15 

0 5 10 15 20 25 30 35 40 45 50 Min. 

Corn Oil 

PUFA (Marine Source) 

Column: 

Temp: 

Carrier Gas: 

Detector: 

1. C14:0 

2. C16:0 

3. C16:1 ω7 

4. C16:4 ω1 

5. C18:1 ω9 

6. C18:1 ω7 

7. C18:1 ω5 

8. C18:2 ω6 

9. C18:3 ω3 

10. C18:4 ω3 

11. C20:1 ω11 

12. C20:1 ω9 

13. C20:1 ω7 

14. C20:2 ω6 

15. C20:3 ω6 

16. C20:4 ω6 


(Part No. 136462) 

160°C to 250°C at 2°C/min 


FID 

Safflower Oil 

1580 1 23 4 

2517 

1844 

17. C20:3 ω3 

18. C20:4 ω3 

19. C22:1 ω11 

20. C22:1 ω9 

21. C21:5 ω3 

22. C22:5 ω3 

23. C22:6 ω3 

 

1. Methyl Palmitate [C16:0] 

2. Methyl Stearate [C18:0] 

3. Methyl Oleate [C18:1 cis] 

4. Methyl Linoleate [C18:2 cis,cis] 

5. Methyl Linolenate [C18:3 cis,cis,cis] 

1. C16:0 Methyl Ester 




 

 

 

0 

3 6 9 12 15 Min. 


(Part No. 19654) 

Temp: 220°C 


Detector: FID 

Column: Heliflex ® AT-FAME, 30m x 0.25mm x 0.25µm 

(Part No. 12436) 

Temp: 220°C 


Detector: FID 

480 



FAMEs 

1 

3 

4 5 

Sesame Oil 


2. Methyl Palmitoleate [C16:1] 





1575 

 

 

 

 

Olive Oil 


2. Methyl Palmitoleate [C16:1] 





1012 

2 

6 

 

 

0 2 4 6 8 10 Min. 


(Part No. 19655) 

Temp: 220°C 


Detector: FID 

 


(Part No. 19654) 

Temp: 220°C 


Detector: FID 

Sugars 

 

TMS Sugars 

Wax 

Carnuba Wax 

1118 2625 

 

 

 

 

1. l-Arabinose 

2. d-Xylose 

3. β-d-Fructose 

4. α-d-Fructose 

5. β-d-Glucose 

6. d-Galactose 

 


Packing: 3% OV-101 on Chromosorb ® W-HP, 80/100 

(Part No. 12019) 

Temp: 

160°C to 250°C at 10°C/min 


Detector: FID 

Aromatics 

 

 

BTEX 

 

 

 

 

1810 

1. Benzene 

2. Toluene 


4. p-Xylene 

5. m-Xylene 

6. o-Xylene 

 

 


(Part No. 16367) 

Temp: 




 

 

 

 

 

Aromatics and MTBE 

 

 

1. MTBE 

2. Methanol 

3. Benzene 

4. Toluene 


6. p-Xylene 

7. m-Xylene 

8. o-Xylene 

9. Styrene 

1939 


 


(Part No. 19659) 

Temp: 


Carrier Gas: Helium at 2.5mL/min (21.9 cm/sec) 


 

Column: 

Temp: 

Carrier Gas: 

Detector: 

 


(Part No. 19654) 



FID 




481


Aromatics 

1 

Substituted Benzenes 

3 

1365 

2 

Substituted Benzenes 

10 

1368 

2 

4 

5 6 

1. Toluene 


3. p-Xylene 

4. m-Xylene 

5. Isopropylbenzene 

6. o-Xylene 

1 

4 

3 5 

78 9 

6 

1. Benzene 

2. Toluene 


4. p-Xylene 

5. m-Xylene 

6. o-Xylene 



9. 1,3,5-Trimethylbenzene 


0 3 6 9 12 15 Min. 


(Part No. 972560) 

Temp: 70°C 


Detector: FID 

0 2 4 6 8 10 12 Min. 


(Part No. 9951302) 

Temp: 


Carrier Gas: Hydrogen at 6mL/min 

Detector: FID 

Sulfur Gases 

6 

5 

2 

3 

Sulfur Gases in Air 

1. Hydrogen Sulfide 

2. Carbonyl Sulfide 

3. Sulfur Dioxide 

4. Methyl Mercaptan 

5. Methyl Sulfide 

6. Dimethyl Disulfide 

1067 

Fuel 

 

 

 

 

 

 

Diesel Fuel 

1. n-Decane 

2. n-Dodecane 




6. n-Eicosane 

7. n-Docosane 


2623 

1 

4 

 

 

petroleum & petrochemical 

0 1 2 3 4 5 6 7 Min. 

Column: 30" x 1/8" o.d., PTFE (Part No. 9781) 

Packing: 18" Super Q, 80/100 (Part No. 2735) 

Temp: 



Detector: FPD 

 

 

 

 

 

 

 

Jet Fuel A 

1. n-Nonane (C9) 

2. n-Decane (C10) 

3. n-Undecane (C11) 

4. n-Dodecane(C12) 

5. n-Tridecane (C13) 

6. n-Tetradecane (C14) 

7. n-Pentadecane (C15) 

8. n-Hexadecane (C16) 

 

 


(Part No. 16367) 

Temp: 


Carrier Gas: Helium at 1mL/min (33cm/sec) 


Kerosene 

2627 2629 

 

1. n-Nonane (C9) 

2. n-Decane (C10) 

 

3. n-Undecane (C11) 

 

4. n-Dodecane(C12) 

5. n-Tridecane (C13) 

6. n-Tetradecane (C14) 

7. n-Pentadecane (C15) 

 

8. n-Hexadecane (C16) 

 

 

 

 


(Part No. 16368) 

Temp: 




 

Column: Heliflex ® AT-1ht, 30m x 0.25mm x .10µm 

(Part No. 16368) 

Temp: 




482 



Fuel 

Texas Crude Oil 

 

 

1. n-Decane 


22. n-Nonacosane 

 

2. n-Undecane 

11. Phytane 

23. n-Triacontane 

3. n-Dodecane 

12. n-Nonadecane 

24. n-Hentriacontane 

 


13. n-Eicosane 

25. n-Dotriacontane 

 


14. n-Heneicosane 

26. n-Tritriacontane 

 


15. n-Docosane 

27. n-Tetratriacontane 

 


16. n-Tricosane 

28. n-Pentatriacontane 

8. n-Heptadecane 


29. n-Hexatriacontane 

9. Pristane 

18. n-Pentacosane 

30. n-Heptatriacontane 

 

19. n-Hexacosane 

31. n-Octatriacontane 

20. n-Heptacosane 

32. n-Nonatriacontane 

21. n-Octacosane 

33. n-Tetracontane 

 

 

 

 

 

 

Column: Heliflex ® AT-1ht, 15m x 0.25mm x 0.10µm 

 

 

(Part No. 16367) 

Temp: 




2626 

Unleaded Gasoline 

234 678 14 

9 

10 

1 

5 

11 

12 

13 

15 

16 

18 

17 

0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 Min. 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

19 

 

21 

20 

22 

 

 

 

 

 

23 

1. Isobutane 

2. n-Butane 

3. Isopentane 

4. Pentane 

5. 2,3-Dimethylbutane 

24 

25 

1. n-Butane 

2. Isobutane 

3. n-Pentane 

4. 2-Methylpentane 


6. n-Hexane 

7. Methylcyclopentane 

8. 2,4-Dimethylpentane 

9. Benzene 

Column: 

Unleaded Gasoline 

Temp: 

Carrier Gas: 

Detector: 



8. Hexane 

9. 2,4-Dimethylpentane 

10. Benzene 

11. 2-Methylhexane 


13. n-Heptane 

14. Toluene 




12. n-Heptane 

13. Toluene 


15. m-Xylene 

16. p-Xylene 

17. 2-Methyloctane 

18. o-Xylene 

16. m-Xylene 

17. p-Xylene 

18. o-Xylene 

19. 1-Methyl-3-Ethylbenzene 







Heliflex ® AT-Petro, 100m x 0.25mm x 0.50µm 

(Part No. 13948) 



FID 

19. n-Nonane 



22. n-Decane 

23. n-Undecane 



26. Dimethylnaphthalenes 


(Part No. 13639) 

Temp: 



Split Ratio: 100:1 

Detector: FID 

1859 

2041 





483


Gases 

C1-C2 Hydrocarbons 

1451 

1 2 3 

4 

5 

6 

C1-C5 Paraffins 

1728 

1 

2 

3 

1. Air 

2. Methane 

3. Carbon Dioxide 

4. Ethylene 

5. Ethane 

6. Acetylene 

1. Methane (0.1894%) 

2. Ethane (0.0965%) 

3. Propane (0.0989%) 

4. Isobutane (0.1019%) 

5. Butane (0.1019%) 

6. n-Pentane (0.2002%) 

4 

5 6 

0 2 4 6 8 Min. 

Column: 5ft x 1/8" o.d., SS (Custom Part No. C-5000*) 

Packing: HayeSep ® T, 80/100 (Part No. 2813) 

Temp: 32°C 


Detector: TCD at 180°C 

*Custom column. Must specifiy dimensions, packing and GC. 

0 3 6 9 12 Min. 

Column: 10ft x 1/8" o.d., SS (Part No. 28301PC) 

Packing: HayeSep ® D, 100/120 (Part No. 2830) 

Temp: 

120°C to 200°C at 20°C/min 


Detector: FID 

 

 

 

C1-C2 Hydrocarbons 

1. Methane (1%) 

2. Acetylene (1%) 

3. Ethylene (1%) 

4. Ethane (1%) 

1727 

2 

1 

3 

4 5 6 

7 8 9 

Hydrocarbons, Sulfur Gases 

10 11 

1. Air 

2. Methane 


4. Acetylene 

5. Ethylene 

6. Ethane 

7. Water 



10. Propylene 

11. Propane 

1772 


 



Temp: 80°C 


Detector: FID 

 

 

 

 

 

 

 

 

 

Hydrocarbons 

 

1. Propane 

2. Propylene 

3. Isobutane 

4. 1-Butene 

5. n-Butane 

6. Isobutylene 

7. cis-2-Butene 

8. trans-2-Butene 

9. 1,3-Butadiene 

10. Isopentane 

11. n-Pentane 

1862 

0 2 4 6 8 Min. 


Packing: HayeSep ® Dip, 100/120 (Part No. 2833) 

Temp: 100°C 


Detector: TCD 

1 2 5 

6 

3 

7 

4 

8 

9 

10 

Hydrocarbons, Sulfur Gases 

11 

1. Air 

2. Methane 


4. Ethylene 

5. Acetylene 

6. Ethane 



9. Propylene 

10. Propane 

11. Propadiene 

1738 

 

 


Packing: 0.19% Picric Acid on Carbograph 2, 80/100 

(Part No. 1736) 

Temp: 50°C 


Detector: TCD 

0 2 4 6 Min. 


Packing: HayeSep ® Q, 80/100 (Part No. 2801) 

Temp: 90°C 



484 



Gases 

Scott Mix 216 

1726 

 

 

Gas Mixture 

1452 

1 

2 

3 

4 

5 6 7 

1. Nitrogen (Balance) 

2. Carbon Monoxide (1%) 


4. Carbon Dioxide (1%) 

5. Acetylene (1%) 

6. Ethylene (1%) 

7. Ethane (1%) 

 

 

 

 

 

1. Nitrogen 

2. Carbon Monoxide 

3. Methane 


5. Acetylene 

6. Ethylene 

7. Water 

8. Ethane 

 

0 11 Min. 

Column: 20ft x 1/8" o.d., Nickel (Part No. 27082PC) 


Temp: 




 


Packing: Hayesep ® DB, 100/120 (Part No. 2836) 

Temp: 120°C 


Detector: TCD 

Alcohols 

 

 

 

 

 

Blood Test Mix 


2. Acetone 

3. Methanol 

4. Isopropanol 

5. Ethanol 

6. n-Propanol 

Bourbon 

1244 4 7 

2797 

6 



3. Methanol 

4. Ethanol 

5. n-Propanol 

6. Isobutanol 

7. Amyl Alcohol + 

2 

8 

Isoamyl Alcohol 


5 

1 3 

 

Column: 

Temp: 

Carrier Gas: 

Detector: 

1 

3 

2 

5 

4 6 

8 

7 


(Part No. 9951302) 



FID 

10 

9 

11 

12 

13 

0 1 2 3 4 5 6 7 8 9 Min. 

14 

17 

18 

15 

16 

19 


0 2 4 6 8 10 12 14 16 18 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 

1. n-Pentane (solvent) 

2. Methanol 

3. Ethanol 


5. 2-Butanol 

6. 1-Propanol 


8. 2-Propen-1-ol 

9. 1-Butanol 

10. 4-Methyl-2-pentanol 


(Part No. 137382) 

50°C (1 min hold) to 90°C (0 min hold) at 10°C/min, 

then to 225°C (6 min hold) at 20°C/min 


FID 



13. 2-Ethyl-1-butanol 

14. 1-Hexanol 

15. 2-Octanol 

16. 1-Heptanol 

17. 2-Ethyl-1-hexanol 

18. 1-Octanol 

19. 1-Decanol 


(Part No. 19653) 

Temp: 50°C (2 min hold), 20°C/min to 220°C 


Detector: FID 

1002 

solvents & chemicals 




485


Alcohols 



 

 

 

 

 

 

 

1007 

1. Methanol 

2. 2-Propanol 

3. 1-Propanol 

4. 2-Butanol 


6. 1-Butanol 


8. 1-Pentanol 


10. 1-Hexanol 

11. Cyclohexanol 

12. 3-Methylcyclohexanol 


1. Methanol 

2. Ethanol 

3. 2-Propanol 

1 

2 

4 5 

3 6 

8 

7 9 

10 

1112 

13 14 

4. 2-Methyl-2- 

propanol 

5. 1-Propanol 

15 

16 

17 18 

6. 2-Butanol 



9. 1-Butanol 

10. 2,2-Dimethyl-1-propanol 


12. 2-Pentanol + 3-Pentanol 





17. 1-Hexanol 


1255 

 

Column: 

Temp: 

Carrier Gas: 

Detector: 

1 

3 

2 


(Part No. 19651) 

40°C to 120°C at 10°C/min 

Helium at 1.09mL/min (26.7cm/sec) 

FID 

78 

5 6 

4 


9 

1. Methanol 

2. Ethanol 

3. 1-Propanol 


5. 1-Butanol 


7. 1-Pentanol 


9. 1-Hexanol 

2596 

0 1 2 3 4 5 6 7 8 9 10 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 

1 

4 

3 

2 

5 

6 


(Part No. 16843) 



FID 

7 8 

Aromatic Alcohols 

1249 

1. α,α-Dimethylbenzyl Alcohol 

2. dl-α-Methylbenzyl Alcohol 


4. 2-Phenylethyl Alcohol 

5. 3-Phenyl-1-propanol 

6. Cinnamyl Alcohol 

7. Phenyl-1,2-ethanediol 

8. Benzhydrol 


0 

2 4 6 8 10 12 14 16 18 20 Min. 0 2 4 6 8 10 12 14 Min. 

Column: Heliflex ® AT-Wax, 30m x 0.32mm x 1.00μm 

(Part No. 137382) 

Temp: 




4 5 C1-C6 Alcohols 

6 7 8 9 

2 3 

1 

1. Methanol 

2. Ethanol 

3. 2-Propanol 

4. 1-Propanol 


6. 1-Butanol 


8. 1-Pentanol 

9. 1-Hexanol 

2598 

Column: 

Temp: 

Carrier Gas: 

Detector: 

Amines 

1 

2 3 

4 

* 

5 

* 

6 


(Part No. 9951102) 

120°C to 215°C at 10°C/min 


FID 

Aliphatic Amines 

7 

8 

9 10 11 

1. Methylamine 

2. Dimethylamine 

3. Ethylamine 

4. Trimethylamine 

5. Isopropylamine 

6. n-Propylamine 

7. t-Butylamine 

8. Diethylamine 

9. sec-Butylamine 

10. Isobutylamine 

11. n-Butylamine 

* Impurity 

1814 

0 1 2 3 4 5 6 7 8 9 10 Min. 


(Part No. 13659) 

Temp: 

40°C to 120°C at 10°C/min 



0 2 4 6 8 10 Min. 


Packing: 4% Carbowax ® 20M + 0.8% KOH on Carbograph 1, 

60/80 (Part No. 1735) 

Temp: 



Detector: FID 

486 



Amines 

Polyamines 

2248 

2 

4 

Ethylamines 

1095 

 

 

1. Putrecine 

2. Cadaverine 

1 

3 

5 

1. Ammonia 

2. Ethylamine 

3. Ethanol 

4. Diethylamine 

5. Triethylamine 

 

Column: Heliflex ® AT-CAM, 30m x 0.32mm x 0.25µm 

(Part No. 13024) 

Temp: 140°C 


Detector: FID 

0 2 4 6 8 10 Min. 


Packing: 28% AT-223 + 4% KOH on Gas Chrom R, 80/100 

(Part No. 49259) 

Temp: 130°C 


Detector: TCD 

Gases 

Ammonia 

Sulfur Compounds 

2 3 

1770 

 

1800 

 

1. Air 

2. Ammonia (35%) 

3. Water (65%) 

 




4. Methyl Mercaptan 

1 

 

0 1 2 3 Min. 


Packing: HayeSep ® P, 60/80 (Part No. 2803) 

Temp: 80°C 


Detector: TCD 

1 

2 3 

Carbon Dioxide and Nitrous Oxide 

1. Air 


3. Nitrous Oxide 

1484 

 


Packing: Carbograph 1SC, 40/60 (Part No. 1734) 

Temp: 35°C 


Detector: FPD 


0 2 Min. 


Packing: Chromosorb ® 101, 80/100 (Part No. 2406) 

Temp: 45°C 


Detector: TCD 





487


Gases 

Permanent Gases 

CTR I - Air, CO, CH4, CO2, O2, N2 

1 

2 

3 

4 

5 

1. Hydrogen 

2. Oxygen 

3. Nitrogen 

4. Methane 


2134 

1 3 

2 

4 

5 

1. Air and Carbon Monoxide 

(Inner Column) 

2. Methane (Inner Column) 

3. Carbon Dioxide (Inner Column) 

4. Oxygen (Outer Column) 


6. Methane (Outer Column) 

7. Carbon Monoxide (Outer Column) 

6 7 

1059 

0 1 2 3 4 5 Min. 


Packing: Molecular Sieve 13X, 80/100 (Part No. 5638) 

Temp: 35°C 


Detector: TCD 

0 1 2 3 4 5 6 7 8 9 Min. 

Column: CTR I (Part No. 8700) 


Inner Column: 6ft x 1/8" Packed with Porous Polymer Mixture 

Temp: 

Ambient 


Detector: TCD 

1 2 3 

N2, CO2, N2O, HS CO2, COS, SO2 

1771 

 

1478 

5 


2. Carbon Dioxide (2%) 

3. Nitrous Oxide (3%) 

4. Water (0.5%) 

5. Hydrogen Sulfide (3%) 

 

 

1. Air, Carbon Monoxide 


3. Water 



4 

 

0 7 Min. 

 




Temp: 80°C 


Detector: TCD 

1 

2 

3 

4 

CH4, CO2, N2O 

1. Air 

2. Methane 



1712 


Packing: Porapak ® Q, 80/100 (Part No. 2701) 

Temp: 75°C 


Detector: TCD 

1 

2 

Hydrogen/Air 

3 

1. Hydrogen 

2. Nitrogen 

3. Oxygen 

4. Argon 

2460 

4 

0 1 2 Min. 

0 1 

2 

3 

4 5 6 Min. 


Packing: Carbosphere, 80/100 (Part No. 5682) 

Temp: 200°C 


Detector: TCD 



Temp: 25°C 


Detector: TCD 

*Custom column. Must specify dimensions, packing, and GC. 

488 



Gases 

1 

5 

MAPP Gas 

Nitric Oxide 

2021 1 

2014 

2 3 

4 

6 7 8 

1. Air 

2. Methane 


4. Ethane 

5. Propylene 

6. Propane 

7. Propadiene 

8. Methyl Acetylene 

2 

3 


2. Argon 

3. Nitric Oxide (0.58%) 

0 2 4 6 8 Min. 


Packing: HayeSep ® R, 100/120 (Part No. 2808) 

Temp: 80°C 


Detector: TCD 


0 2 4 6 8 Min. 


Packing: HayeSep ® DB, 100/120 (Part No. 2836) 

Temp: 22°C 



1 

Permanent Gases 

1450 

2 

1 

4 

Reactive Gas Mixture 

1447 

3 

2 

4 

5 

1. Nitrogen 

2. Oxygen 


4. Methane 


3 

5 

6 

7 

8 

1. Air 

2. Methane 



5. Ammonia 

6. Ethane 



0 3 6 9 12 15 18 Min. 

0 5 10 15 20 Min. 

Column: 20ft x 1/8" o.d., Ni (Part No. 27082PC) 


Temp: 25°C 


Detector: TCD 

1 

2 


Packing: HayeSep ® Q, 80/100 (Part No. 2801) 

Temp: 50°C 


Detector: TCD 

Room Air Scott Mix 234 

 

1706 

1. Nitrogen (78%) 

2. Oxygen (21%) 

3. Argon (0.94%) 

1. Helium (Balance) 

2. Hydrogen (4%) 

3. Oxygen (4%) 


2020 


3 

 

 

0 2 4 Min. 

 

 


Packing: Hayesep ® DB, 100/120 (Part No. 2836) 

Temp: 

-66°C 


Detector: TCD 


Packing: HayeSep ® DB, 100/120 (Part No. 2836) 

Temp: 22°C 


Detector: TCD 




489


Glycols/Diols 

Diols 

1 

EG and Diols 

1 2 

1225 

2 

1275 

3 

4 5 6 7 

1. 1,2-Butanediol 

2. 1,2-Propanediol 





7. 1,5-Pentanediol 

3 

4 



3. 1,3 Butanediol 


0 2 4 6 8 10 Min. 


(Part No. 9925252) 

Temp: 150°C 

Carrier Gas: Hydrogen at 0.9mL/min 

Detector: FID 

0 1 2 3 4 5 6 7 Min. 


(Part No. 9951102) 

Temp: 110°C 


Detector: FID 

1 

Ethylene Glycol/2-Chloroethanol 

1839 

1 

2 

3 

5 

Glycol Amines 

1266 

2 3 

1. Water 

2. 2-Chloroethanol 


4 

1. Ethanolamine 


3. Diethylene Glycol 

4. Diethanolamine 

5. Triethylene Glycol 


0 1 2 3 4 Min. 


(Part No. 19684) 

Temp: 120°C 


Detector: FID 

1 

2 

3 4 

5 

6 

Glycol-Diol Mix 

7 

8 







7. Diethylene Glycol 

8. Glycerol 

1087 

0 2.5 5 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 

Solvents 

1 2 3 4 5 6 


(Part No. 9951102) 

130°C to 180°C at 10°C/min 


FID 

7 

8 

9 10 

11 

Aromatics 

12 

1. Benzene 

2. Toluene 


4. p-Xylene 

5. m-Xylene 

6. o-Xylene 







2586 

0 1 2 3 4 Min. 

0 

2 4 6 8 10 1214 16 18 20 22 24 min. 


Packing: Chromosorb ® 101, 100/120 (Part No. 2407) 

Temp: 210°C 


Detector: FID 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 137382) 



FID 

490 



Solvents 

Volatile Organics 

4 

Glycol Ethers 

1878 

1337 

1 

2 

3 4 5 

6 

7 8 

9 10 


2. Chloroform 



5. Benzene 

6. Toluene 





1 

2 

3 

5 

1. Cellosolve Acetate 

2. Methyl Carbitol ® 

3. Carbitol 

4. n-Decane 

5. Butyl Carbitol 

0 4 8 12 16 20 24 28 32 36 40 Min. 


Packing: 1% AT-1000 on Carbograph 1, 60/80 (Part No. 1733) 

Temp: 



Detector: FID 

0 1 2 3 4 5 Min. 


(Part No. 935110) 

Temp: 



Detector: FID 

 

 

 

 

 

 

 

 

 

Aromatics 

 

1. Benzene 

2. Toluene 



5. m-Xylene 

6. p-Xylene 

7. o-Xylene 






2587 

10 

3 

4 

6 

2 78 

5 

1 

9 

Impurities in Methyl Ethyl Ketone 

1. Methanol 

2. Ethanol 

3. 2-Propanol 


5. n-Propanol 

6. 2-Butanol 

7. Acetone 

8. 1-Octene 


10. Methyl Ethyl Ketone 

1221 

 

 

0 5 10 15 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 

1 


(Part No. 13641) 



FID 

Ethylene Glycol, Ethylene Oxide and 

2-Chloroethanol in DMF 

2 3 

4 

5 

6 

1812 


2. Methanol 

3. N,N-Dimethylformamide 

(solvent) 

4. 2-Chloroethanol 

5. Impurity in Solvent 


Column: 

Temp: 

Carrier Gas: 

Detector: 

1 

2 

3 

4 

5 

6 

7 

8 


(Part No. 985130) 

80°C (1 min hold) to 170°C at 7°C/minute 


FID 

Halogenated Aromatics 

1. 2-Bromobutane 

2. 1-Bromobutane 


4. 1,4-Dichlorobutane 

5. 1-Bromohexane 

6. Bromocyclohexane 

7. o-Chlorotoluene 

8. Benzyl Chloride 

1345 


0 2 4 6 8 12 14 16 Min. 

0 1 2 3 Min. 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 19655) 



FID 


(Part No. 935110) 

Temp: 60°C 


Detector: FID 




491


Solvents 

2 

3 

5 

1 10 

7 14 

12 

6 8 13 

9 

4 11 

19 

18 

15 16 

17 

0 3 6 9 12 15 Min. 

23 

20 

27 

26 

21 

22 

25 

24 

28 

29 

30 

31 

Industrial Solvent Mix 

1. Pentane 

2. 2,2,4-Trimethylpentane 


4. Methyl Formate 

5. Acetone 

6. Tetrahydrofuran 


8. 2-Butanone 

9. 2-Propanol 

10. Benzene 

Column: 

Temp: 

Carrier Gas: 

Detector: 


12. 4-Methyl-2-pentanone 

13. 2-Butanol 

14. Toluene 



17. 2-Nitropropane 


19. 1,4-Xylene 


21. 1-Butanol 

22. Nitromethane 



25. 2-Ethoxyethanol 



28. 2-Ethoxyethyl Acetate 

29. 1-Hexanol 

30. 2-Butoxyethanol 



(Part No. 19654) 



FID 

1015 


1. Benzene 

2. Trifluoromethylbenzene 

3. Toluene 

1 

2 

3 

11 12 

14 

10 13 

15 

16 

17 

18 

56 

7 8 19 

4 9 

21 

25 

20 22 27 

23 

24 26 

28 30 

29 

0 2 4 6 8 10 12 14 16 18 20 22 Min. 

1 

2 3 4 

5 

7 

6 

13 14 

11 12 15 

8 9 10 

16 

0 4 8 12 16 20 Min. 



6. 1,3-Xylene 


2. Acetone 



5. Methyl Ethyl Ketone 


7. Benzene 


Substituted Aromatics 

7. 1,2-Xylene 


9. Bromobenzene 

10. 1-Chloro-2-methylbenzene 

11. 1-Chloro-4-methylbenzene 


13. 2-Methyl-2-phenylpropane 



16. 1-Isopropyl-4-methylbenzene 

17. α-Chlorotoluene 


Column: 

Temp: 

Carrier Gas: 

Detector: 

NIOSH Solvents 

9. Chloroform 

10. Tetrachloroethylene 

11. Toluene 

12. p-Dioxane + Ethylene dichloride 

13. p-Xylene 

14. m-Xylene 

15. o-Xylene 


19. 1-Bromo-4-methylbenzene 

20. 4-Chlorostyrene 

21. 1-Bromo-4-chlorobenzene 

22. 1,2,4,5-Tetramethylbenzene 

23. 2,4-Dichlorotoluene 

24. 1-Iodo-4-methylbenzene 

25. Tetrahydronaphthalene 




29. 1,2,4,5-Tetrachlorobenzene 



(Part No. 13959) 



FID 

Column: 20ft x 1/8" o.d., SS (Part No. C-5000*) 

Packing: 10% AT-1000 on Chromosorb ® W-AW, 80/100 

(Part No. 12001) 

Temp: 

60°C (2 min hold) to 160°C at 5°C/minute 


Detector: FID 


1838 

1177 

492 



Test Mixes 

 

 

 

 

 

AT-1ms Test Chromatogram 

 

 

 



3. 1,7-Heptanediol 







 

2578 

0 5 10 15 20 25 30 Min. 

1 

2 

3 

4 

5 

6 

7 

8 

9 

Grob Mix 

10 

11 

12 

1045 

1. n-Decane 

2. n-Undecane 

3. Nonanal 


5. 1-Octanol 

6. Methyl Decanoate 


8. Methyl Undecanoate 

9. Methyl Dodecanoate 

10. 2,6-Dimethylaniline 



Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 15897) 


Helium at 0.65mL/min (23.1cm/sec) 

FID 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 19655) 



FID 

Pyrolysis 

 

Pyrolysis - ABS Plastic 

1. 1,3-Butadiene 

2. Acrylonitrile 

3. Benzene 

4. Toluene 

5. Vinyl Cyclohexene 

6. Styrene 

7. α-Methylstyrene 

8. Benzenepropanenitrile 

9. 1-Benzyl Pyrrole 

2417 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Column: 

Temp: 

Carrier Gas: 

Detector: 

Pyrolysis - Isoprene Rubber 


(Part No. 13638) 



MSD 

1. Butene 

2. 1,3-Pentadiene 

3. Toluene 

4. p-Xylene 

5. Styrene 

6. 1-Ethyl-4-methylbenzene 

7. Limonene 


2415 


 

 

 

 

 

Column: 

Temp: 

Carrier Gas: 

Detector: 


(Part No. 13638) 



MSD 




493

spe applications 

Analgesics 

SPE Extraction of Salicylic and Acetylsalicylic Acids from Synthetic Urine 

10642 

Procedure using GracePure C18-Max, 100mg: 

Sample Treatment – Spike 2mL synthetic urine with 100ppm salicylic acid and 100ppm 

acetylsalicylic acid. 

Conditioning – Rinse device with 3mL methanol followed by 3mL DI water. 

Sample Application – Apply 2mL spike urine. 

Wash – Wash with 2mL 50mM phosphate buffer monobasic, pH 2. 

Elution – Elute with 2mL methanol:water (50:50). 

1 

2 



Column: Alltima HP C18 HiLoad, 5µm, 250 x 4.6mm 

(Part No. 87698) 

Mobile Phase: 5mM Sodium Acetate, pH5.2:Methanol (80:20) 



0 2 4 6 8 10 12 14 16 

18 Min. 


Sedatives 

Procedure using GracePure C18-Aq, 500mg: 

Sample Treatment – Spike 500µL plasma with 10µL of a 0.1mg/mL standard solution. 

Combine with 100µL of 0.1M sodium carbonate buffer. Vortex and centrifuge for 

10 minutes. 

Conditioning – Rinse device 2mL water. 

Sample Application – Apply entire sample. 

Wash – Wash with 2mL water and then 50µL methanol. 

Elution – Elute with 600µL methanol. 

Column: Alltima HP C18, 5μm, 250 x 4.6mm (Part No. 87680) 



%B: 30 80 



Benzodiazepines from Human Plasma 

1 

2 3 

1 2 

3 

0 2 4 6 8 10 12 14 16 18 20 Min. 

4 

4 

1. Oxazepam 

2. Clonazepam 

3. Temazepam 

4. Diazepam 

Before SPE 

Clean-up 

After SPE 

Clean-up 

10606 

10608 

494 



Diuretics 


Sample Treatment – Spike synthetic urine with 8 diuretics to a concentration of 

1.25µg/mL each. 

Conditioning – Rinse device 5mL methanol, followed by 5mL water. 

Sample Application – Apply 15mL spiked urine sample at 1mL/min. 

Wash – Wash with 5mL water. 

Elution – Elute with 2mL methanol. Evaporate solvent and reconstitute in 250µL 

HPLC mobile phase. 

Diuretics from Urine 

1 

2 

3 

4 

5 

6 

7 

8 

10289 

10290 




4. Clopamide 


6. Indapamide 



Column: Alltima C18, 3μm, 100 x 4.6mm (Part No. 81382) 

Mobile Phase: A: 25mM Ammonium Acetate, 0.1%Trifluoroacetic Acid 

B: Acetonitrile, 0.1%Trifluoroacetic Acid 


%B: 20 90 



After SPE 

Concentration 

Before SPE 

Concentration 

0 1 2 3 4 5 6 7 8 9 10 Min. 

St. John’s Wort 

Procedure Using GracePure C18-Max, 1000mg: 

Sample Treatment – Pulverize 300mg St. John’s Wort powder into five, 3mL aliquots 

of methanol:water (80:20). Combine and filter extracts to produce 15mL filtrate. Dilute 

to 30mL with water. 

Conditioning – Rinse device with 5mL methanol followed by 5mL deionized water. 

Sample Application – Apply 2mL filtrate to the top of the SPE device and draw through 

the C18 packed bed. 

Wash* – Wash device with 3mL of deionized water. 

Elution – Elute with 2mL methanol. 

*Repeat load and wash steps consecutively until filtrate is consumed. 

Column: Alltima C18, 3µm 53 x 7mm Rocket (Part No. 50605) 

Mobile Phase: Methanol:0.2% Phosphoric Acid (95:5) 


Detector: VIS at 585nm 

Hypericins from St. John’s Wort 

1 

0 1 

2 

2 

9107 

1. Pseudohypericin 

2. Hypericin 

3 Min. 

nutraceuticals 

Chamomile 

Antioxidants from Chamomile 

9133 


Sample Treatment – Pulverize 1g commercial chamomile tea grounds into 

6mL dioxane:methanol (50:50). Filter extract and dilute to 20mL with water. 

Conditioning – Rinse device with 5mL methanol followed by 5mL water. 

Sample Application* – Apply 1mL filtrate. 

Wash* – Wash with 1mL water. 

Elution – Elute first with 2mL methanol:water (50:50). Elute second, fraction 

and use for this analysis, with 2mL methanol. 

*Before elution step, repeat load and wash steps until filtrate is consumed. 

1 

1. Apigenin-7-Glucoside 


Mobile Phase: Acetonitrile:20mM K2HPO4, pH7.3 (65:35) 


Detector: UV 340nm 

Inj. Vol.: 5µL 

0 1 

2 3 Min. 




495


Echinacea 

Phenolic Acids from Echinacea 

9110 


Sample Treatment – Pulverize 300mg echinacea powder into five, 3mL aliquots of 

methanol. Combine and filter extracts to produce 15mL filtrate. Dilute to 30mL with 

water. 

Conditioning – Rinse device with 5mL methanol followed by 5mL deionized water. 

Sample Application – Apply 2mL filtrate to the top of the SPE device and draw through 

the C18 packed bed. 

Wash* – Wash device with 2mL of deionized water. 

Elution – Elute with 5mL of methanol:water (50:50). 

*Repeat load and wash steps consecutively until filtrate is consumed. 

1 

3 

1. Echinicoside 

2. Chlorogenic Acid 

3. Caffeic Acid 


Mobile Phase: Acetonitrile:10mM K2HPO4, pH2.6 (20:80) 



0 1 

2 

2 Min. 

Kava Kava 

Sedatives from Kava Kava 

9125 

nutraceuticals 


Sample Treatment – Pulverize 300mg commercial kava kava root powder into four, 2mL 

aliquots of methanol:water (80:20). Combine and filter extracts to produce 8mL filtrate. 

Dilute to 10mL with water. 






Column: Alltima C18, 3µm, 53 x 7mm Rocket (Part No. 50605) 

Mobile Phase: Acetonitrile:Isopropyl Alcohol:0.2% Acetic Acid (17:23:60) 




0 1 

1 

3 

4 

2 

2 

3 

5 

1. Methysticin 

2. Yangonin 

3. Kavain 

4. Dihydrokavain 

5. Dihydromethysticin 

4 

5 

Dong Quai 

Vasodilators from Dong Quai 

9132 


Sample Treatment – Pulverize 300mg commercial kava kava root powder into four, 2mL 

aliquots of methanol:water (80:20). Combine and filter extracts to produce 8mL filtrate. 

Dilute to 10mL with water. 






1 

1. Z-ligustilide 


Mobile Phase: Methanol:0.2% Acetic Acid (65:35) 




0 1 

2 3 4 5 Min. 

496 



Additives 

Procedure using GracePure Anion-X, 500mg: 

Sample Treatment – Use 8mL fruit punch and adjust pH to 10 using potassium 

hydroxide. 

Conditioning – Rinse device 10mL water. 

Sample Application – Apply 8mL pH adjusted fruit punch sample. 

Wash – Wash with 20mL water. 

Elution – Elute with 1mL 1.0N hydrochloric Acid followed by 1mL methanol. 

Preservatives from Fruit Punch 

1 

1. Sorbic Acid 


2576 

Column: Heliflex ® AT AquaWax-DA, 30m x 0.25mm x 0.25μm 


Temp: 

200°C (5 min hold) to 230°C (4 min hold) at 5°C/min 



0 

2 4 6 8 10 12 14 Min. 

2 

Carbohydrates 


Sample Treatment – Add 0.167mg/mL each of carbendazim, thiabendazolem, 

triadimenol, triadimefon, flusilazole, diniconazole into 

1mL water. Combine with 1mL Beaujolais red wine. 


Sample Application – Apply 2mL red wine mixture. 

Wash – No wash. 


Column: Alltima HP C18 Amide, 5μm, 250 x 4.6mm 

(Part No. 87734) 



%B: 15 45 45 15 



Fungicides from Red Wine 

1 

2 

3 

Before SPE 

Clean-up 

0 5 10 15 20 25 30 35 40 45 50 55 60 65 Min. 

6 

1. Carbendazim 

2. Thiabendazole 

3. Triadimenol 

4. Triadimefon 

5. Flusilazole 

6. Diniconazole 

10637 

10638 

4 5 

After SPE 

Clean-up 





497


Proteins & Peptides/Reversed Phase 

Prevail SPE Protein Desalting, Cytochrome C and Ribonuclease A Mix in 10mM Sodium Chloride 

Procedure using Prevail C18, 500mg: 

Sample Treatment – Cytochrome-C 1mg/mL, Ribonuclease-a 1mg/mL, Blank = water, 

Salt = 10mM NaCl. 

Conditioning – 1 tube volume of methanol (do not dry). 2 tube volumes of 0.025% 

ammonium hydroxide. Dry tube via vacuum. 

Sample Application – Pipette 1mL of sample (either proteins in water blank or proteins 

in salt solution) onto top of sorbent bed in SPE tube. Run vacuum @ approximately 

-10 kPa, loading sample in a tight band at top of sorbent column, continue to apply 

vacuum (@ -10 kPa) until tube is dry. Larger (2mL +) volumes can be de-salted and 

concentrated. Liquid collected will contain the NaCl 

Elution – Pipette 500µL 0.4% TFA and 500µL Acetonitrile containing 0.4% TFA onto top 

of sorbent bed in spe tube. Run vacuum @ approximately -10 kPa, eluting sample from 

sorbent column, continue to apply vacuum until tube is dry. 

Column: ProSphere 300 C4-300, 5µm, 150 x 4.6mm 

(Part No. 33989) 

Mobile Phase: A: 0.2% Trifluoroacetic Acid B: Acetonitrile 


%B: 25 70 



1 

2 

0 1 2 3 4 5 6 7 8 9 Min. 

3 

1. Chloride 

2. Ribonuclease A 

3. Cytochrome C 

10688 

Protein Extraction of Ribonuclease and Myoglobin 

Procedure using Vydac ® SPE: 

A 3mL SPE cartridge was conditioned with 1mL of Acetonitrile followed by 1mL of 

5% Acetonitrile/0.1% Trifluoroacetic Acid. Ribonuclease and myglobin (100mg each) 

were then loaded in 30% Acetonitrile/0.1% Trifluoroacetic Acid . The cartridge was 

washed with 1mL of 5% Acetonitrile/0.1% Trifluoroacetic Acid to remove weakly bound 

compounds, then 1mL of 30% Acetonitrile/0.1% Trifluoroacetic Acid followed by 1mL of 

60% Acetonitrile/0.1% Trifluoroacetic Acid. HPLC analysis of the 5% Acetonitrile wash 

(A) revealed only a small amount of ribonuclease. Most of the ribonuclease eluted in the 

30% Acetonitrile wash (B). The myoglobin eluted almost entirely in the 60% Acetonitrile 

wash (C). 

Solid Phase Extraction Example 

A. 5% ACN 

1 

2 

1. Ribonuclease 

1. ribonuclease 

2. Myoglobin 

2. myoglobin 

life science 


Mobile Phase: A: 0.1% Trifluoroacetic Acid B: Acetonitrile 


%B: 15 70 

B. 30% ACN 

C. 60% ACN 

0 2 4 6 8 10 Min. 

A 3 mL SPE cartridge was conditioned with 1 mL of ACN followed by 1 mL of 5% 

ACN/0.1% TFA. Ribonuclease and myglobin (100 mg each) were then loaded in 30% 

ACN/0.1% TFA . The cartridge was washed with 1 mL of 5% ACN/0.1% TFA to remove 

weakly bound compounds, then 1 mL of 30% ACN/0.1% TFA followed by 1 mL of 60% 

ACN/0.1% TFA. HPLC analysis of the 5% ACN wash (A) revealed only a small amount 

of ribonuclease. Most of the ribonuclease eluted in the 30% ACN wash (B). The 

myoglobin eluted almost entirely in the 60% ACN wash (C). 

Column: 

Gradient: 

VYDAC 214TP5405 (C4, 5 µm, 4.6 mm i.d. x 50 mm) 

15 to 70% ACN over 10 min, with 0.1% TFA 

498 



Pesticides 


Sample Treatment – Homogenize 5 grams green bell pepper with 25mL methanol, spike 

with 0.5μg each of 16 chlorinated pesticides. Filter with 75mL filter column (Part No. 

210775). Dilute to 200mL with DI water and shake. 

Conditioning – 2 x 5mL methanol, then 5mL DI water. 

Sample Application – Attach 75mL reservoir (Part No. 210575) with a syringe adapter 

(Part No. 210705) and apply entire sample. 

Wash – No wash, apply vacuum for 5 min. 

Elution – Elute chlorinated pesticides with 2 x 1mL n-hexane/CH2Cl2 (70:30), 

evaporated this to dryness and reconstituted with 1mL n-hexane. 

Chlorinated Pesticides in Vegetables 

1. a-BHC 

2. b-BHC 

3. g-BHC 

4. d-BHC 

5. Heptachlor 

6. Aldrin 



9. p,p’-DDE 

7 8 

5 

1 23 4 6 9 10 1215 

11 16 13 

2780 

10. Dieldrin 

2789 

11. Endrin 


13. p,p’-DDD 


15. p,p’-DDT 


14 

After SPE 

Clean-up 

Column: AT 5ms, 0.25µm, 30m x 0.25mm (Part No. 15807) 

Temp: 

160°C (0 min hold) at 10°C/min, then to 275°C at 5°C/min 


Detector: ECD at 310°C 

Before SPE 

Clean-up 

0 5 10 15 20 25 30 35 Min. 


Sample Treatment – Weighed out 5 grams dry soil, spike with 1μg each of 16 

chlorinated pesticides, add added 25mL methanol and homogenize. Filter with 75mL 

filter column (Part No. 210775). Dilute to 200mL with DI water and shake. 

Conditioning – 2 x 5mL methanol, then 5mL DI water. 


(Part No. 210705) and apply entire sample. 


Elution – Elute chlorinated pesticides with 2 x 1mL n-hexane/CH2Cl2 (70:30), evaporate 

to dryness and reconstitute with 1mL iso-octane. 

Chlorinated Pesticides in Soil 

1. a-BHC 

2. b-BHC 

3. g-BHC 

4. d-BHC 

5. Heptachlor 

6. Aldrin 



9. p,p’-DDE 

10. Dieldrin 

1 2 

3 

4 

2779 

11. Endrin 

2788 


13. p,p’-DDD 


15. p,p’-DDT 


7 8 

5 

9 11 12 

6 10 

13 1415+16 After SPE 

After SPE 

Clean-up 


Temp: 

160°C (0 min hold) at 10°C/min, then to 275°C at 5°C/min 



Before SPE 

Clean-up 

0 5 10 15 20 25 30 35 Min. 

PCBs in Transformer Oil 

Procedure using GracePure Florisil ® , 1000mg: 

Sample Treatment – Dissolve 0.25grams of transformer oil spiked with Aroclor 1254 (at 

a concentration of 50mg/kg) in 20mL n-hexane. 

Conditioning – 2 x 5mL n-hexane, making sure the cartridge does not dry. 


(Part No. 210705) and run sample through conditioned Florisil ® tube, aspirating all the 

solution from the tube. Evaporate the volume down to 4mL and analyzed by GC/ECD. 


Elution – No elution, collect sample load. 

1. Aroclor 1254 

2784 

environmental 


Temp: 




Clean-up 

0 5 10 15 20 25 30 35 40 Min. 




499


Pesticides 

Procedure using GracePure C18-Fast, 1000mg: 

Sample Treatment – Weigh out 2.5 grams salmon, spike with 2.5μg Aroclor 1242, add 

25mL acetonitrile, homogenize for 30 seconds. Filter with 75mL filter tube (Part No. 

210775). Rinse salmon again with 5mL acetonitrile. Bring up to 100mL with deionized 

water. 

Conditioning – Rinse with 6mL hexanes, 6mL acetone, 2 x 6mL methanol, 2 x 6mL 

water. 

Sample Application – Attach 75mL reservoir, apply entire sample. 

Wash – 2 x 5mL water. Dry columns for 10 minutes at full vacuum. 

Elution – Elute PCBs with 2 x 3mL 3% toluene/97% n-hexane. Evaporate 

solvent and reconstitute in 1mL n-hexane. 

PCBs in Salmon 

1. Aroclor 1242 

After SPE 

Clean-up 

2781 

2787 


Temp: 




0 5 10 15 20 25 30 35 40 Min. 

Before SPE 

Clean-up 


Conditioning – Rinse device with 5mL of methanol followed by 5mL deionized water. 

Sample Application – Pass 100mL–500mL (containing 1% methanol) of water sample 

through the device at 20mL/minute. 

Wash – Wash device with 10mL of deionized water then 10mL of methanol:deionized 

water (20:80). Remove excess by passing air through the device for two minutes. 

Elution – Elute with 2mL of hexane:ethyl acetate (70:30). Pass extract through 2g–3g 

sodium sulfate to remove residual water. 

Column: AT Pesticide 20m x 0.53mm x 0.60µm Capillary GC 

Column (Part No. 16846) 

Temp: 210°C 

Carrier Gas: Helium, 35cm/sec 

Detector: ECD 

Chlorinated Pesticides from Water 

3 

1 2 

5 

4 

7 

6 8 9 

10 12 

11 

13 

14 

18 

15 

16 17 

0 9 18 Min. 

1668 

Recovery (as % Standard) 

1. a-BHC (104%) 

2. Lindane (109%) 

3. β-BHC (106%) 

4. Heptachlor (92%) 

5. δ-BHC (110%) 

6. Aldrin (98%) 

7. Heptachlor Epoxide (107%) 

8. α-Endosulfan (105%) 

9. p,p'-DDE (79%) 

10. Dieldrin (102%) 

11. Endrin (104%) 

12. p,p'-DDD (92%) 

13. β-Endosulfan (103%) 

14. p,p'-DDT 

15. Endrin Aldehyde (102%) 

16. Endosulfan Sulfate (103%) 

17. Methoxychlor (99%) 

18. Endrin Ketone (104%) 

environmental 


Sample Treatment – Spike 500mL tap water with 125µL carbamate solution for final 

concentration of 25ppb. 

Conditioning – Rinse with 3mL acetonitrile:water (80:20) followed by 3mL water. Dry 

with vacuum. 

Sample Application – Apply 500µL sample. 

Wash – 2 x 3mL water. 

Elution – Elute with 4 x 1mL acetonitrile:water (80:20) 

Column: Platinum EPS C18, 5μm, 250 x 4.6mm (Part No. 32246) 

Mobile Phase: A: DI water B: Acetonitrile 


%B: 25 25 50 50 25 



Carbamate Pesticides from Water 

1. Aldicarb sulfoxide 

2. Methomyl 

3. 3-Hydroxycarbofuran 

4. Propoxur 

5. Carbaryl 

6. Methiocarb 

2 3 

1 

0 5 10 15 20 25 Min. 

4 

5 

6 

After SPE 

Concentration 

Before SPE 

Concentration 

10599 

10600 

500 



PAHs 

Procedure using GracePure C18-Max: 

Sample Treatment – 500mL of water and add 75mL of 2-propanol, 

spiked with 20uL of PAH mixture at 2000ug/mL of each PAH, which corresponds to 

40ug/500mL water, or 80ppb of each PAH. 

Conditioning – 2 x 5mL of methanol, then 5mL of 85% water/ 

15% 2-propanol. 

Sample Application – Attach 75mL reservoir (Part No. 210575) with a 

syringe adapter (Part No. 210705) and apply entire sample. Dry 

columns for 30 minutes at full vacuum. 


Elution – Elute PAHs with 3 x 1mL CH2Cl2 and adjusted the volume 

to 1mL by evaporating under a gentle nitrogen stream. 

PAHs in Water 

1 

2 3 

4 




4. Fluorene 


6. Anthracene 


8. Pyrene 

5 6 

7 8 

9. Benzo(a)anthracene 

10. Chrysene 

11. Benzo(b)fluoranthene 

12. Benzo(k)fluoranthene 

13. Benzo(a)pyrene 

14. Indeno(1,2,3-cd)pyrene 

15. Dibenzo(a,h)anthracene 

16. Benzo(ghi)perylene 

14 15 

16 

11 

9 

10 12 13 

2771 

2772 


Temp: 


then to 300°C (0 min hold) at 3°C/min, then to 325°C 

(3 min hold) at 25°C/min 



After SPE 

Clean-up 

Before SPE 

Clean-up 

0 5 10 15 20 25 30 35 40 45 50 Min. 

PAHs in Toasted Oat Cereal 

Procedure using GracePure C18-Max: 

Sample Treatment – 500mL of water and add 75mL of 2-propanol, spiked with 20uL of 

PAH mixture at 2000ug/mL of each PAH, which corresponds to 40ug/500mL water, or 

80ppb of each PAH. 

Conditioning – 2 x 5mL of methanol, then 5mL of 85% water/15% 2-propanol. 


(Part No. 210705) and apply entire sample. Dry columns for 30 minutes at full vacuum. 

Wash – 2 x 500µL of 85% water/15% isopropyl alcohol. Vacuum 10 min. 

Elution – Elute PAHs with 3 x 1mL CH2Cl2 and adjusted the volume to 1mL by 

evaporating under a gentle nitrogen stream. 




4. Fluorene 


6. Anthracene 


8. Pyrene 

1 

8 


10. Chrysene 







16 

15 

14 

2775 

2791 

2 3 

4 

5 6 7 

11 13 

9 12 

10 


Temp: 






Before SPE 

Clean-up 

0 5 10 15 20 25 30 35 40 45 50 Min. 

After SPE 

Clean-up 

environmental 




501


PAHs 

PAHs in Cooking Oil 

2776 

2792 

Procedure using DVB Extract-Clean, 500mg/4mL: 

Sample Treatment – Weigh out 1 grams canola oil, spike with 20μg of each PAH, add 

10mL n-hexane. 

Conditioning – 10mL toluene, applied vacuum until toluene removed, then 5mL n-hexane. 

Sample Application – Aspirate the entire canola oil/hexane/PAH solution through the tube. 

Wash – 5mL n-hexane. 

Elution – Elute PAHs with 4 X 2.5mL toluene and adjusted the sample volume to 0.5mL 

(500μL) by evaporating under dry nitrogen stream and gentle heat. 




4. Fluorene 


6. Anthracene 


8. Pyrene 


10. Chrysene 







15 16 

14 


Temp: 






1 

8 

2 

3 4 56 7 

9 10 1112 13 

After SPE 

Concentration 

Before SPE 

Concentration 

0 5 10 15 20 25 30 35 40 45 50 55 Min. 

environmental 

Nitroaromatics 


Sample Treatment – Spike 100g soil with 7.5µg/g of each analyte. Combine soil with 

1000mL deionized water, shake for 10 minutes, and filter. 


Sample Application – Aspirate 1000mL water sample through SPE at flow rate 1- 

5mL/min. 

Wash – No wash. Air dry for 15 seconds. 

Elution – Elute with three 1mL aliquots of methanol:water (50:50). Air dry 

for 15 seconds between each elution. 

Column: Adsorbosphere UHS C18, 5µm, 150m x 4.6mm 

(Part No. 288118) 




Nitroaromatics and Naphthols from Soil 

1 

Before SPE 

Concentration 

0 5 10 15 20 25 30 35 Min. 

2 

3 

4 

5 

6 


2. 1-Naphthol 

3. 2-Naphthol 

4. 2-Nitrotoluene 



After SPE 

Concentration 

10597 

10598 

502

Grace Catalog 2008

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