Grace Catalog 2008

analytical hplc
hplc instruments
ELSD Advantages Over Other LC Detectors
ELSD improves baseline stability and detection sensitivity
compared to refractive index (RI).
Solvent
Front
Sugar Alcohols
1
1
2
3
2 3
ELSD
RI
10627
10635
1. Xylitol (200ppm)
2. Sorbitol (200ppm)
3. Dulcitol (200ppm)
ELSD shows what may be missing from your UV
chromatogram.
Plant Oil Extract
0 10 20 30 40 50 60
ELSD
10656
10657
UV at 254nm
70 Min.
0 2 4 6 8 10 12 14 16
18 Min.
Column: Alltech ® Prevail Carbohydrate ES, 5µm,
4.6 x 250mm (Part No. 35101)
Mobile Phase: Acetonitrile:Water (79:21)
Flow Rate: 1.0mL/min
Column Temp: 30°C
Column: Alltech ® Alltima C18, 3μm, 2.1 x 150mm
(Part No. 43852)
Mobile Phase: A: 0.1% Formic Acid B: Methanol
Gradient: Time: 0 40
%B: 60 100
Flow Rate: 0.25mL/min
Column Temp: 30°C
Use ELSD in parallel with UV/Vis and MS for maximum
structural and concentration information.
1
Steroid Hormone Formulation
1. Povidone (8 µg)
2. Chlormadinone Acetate (1.5 µg)
3. Mestranol (3 µg)
2
2
3
2
ELSD
1
3
MS
1
3
UV at 254nm
0 2 4 6 8 10 12 14 16 18 20 Min.
10639
10641
10640
Column: Vydac ® Denali ® 238DE C18, 3µm, 2.1 x 50mm
(Part No. 238DE3205)
Mobile Phase: 0.2% Formic Acid in Acetonitrile:Water (55:45)
Flow Rate: 0.2mL/min
Column Temp: Ambient
ELSD obtains a more accurate representation of sample
mass than UV/Vis.
1. Mephobarbital (500ppm)
2. Secobarbital (500ppm)
1:1 Mixture
UV at 230nm
0 2 4 6 8 10 12 14 16 18 20 Min.
1
2
1
2
ELSD
10616
10617
Column: Alltech ® Alltima HP C18 HL, 5µm, 4.6 x 250mm
(Part No. 87698)
Mobile Phase: Acetonitrile:Water (30:70)
Flow Rate: 1.0mL/min
Column Temp: 45°C
technical assistance
Contact Tech Support: Phone: 1.800.255.8324 (North America)
Email: contact.alltech@grace.com
Online: www.discoverysciences.com
more applications
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chromatogram database visit
www.discoverysciences.com/chromdb/
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