Grace Catalog 2008

hplc applications
Miscellaneous
Phospholipids
10314
1
2
3
4
1. 1,2-Phosphatidyl-ethanolamine
2. Phosphatidyl-dl-glycerol
3. Phosphatidylinositol
4. 1,2-Phosphatidyl-l-serine
5. Phosphatidylcholine
6. Sphingomyelin
5 6
Column: Alltech ® Prevail Silica, 3µm, 150 x 4.6mm (Part No. 99275)
Mobile Phase: A: Isopropanol B: Hexane C: Water
Gradient: Time: 0 7 15
%B: 40 40 40
%C: 2 8 8
Flow Rate: 1.0mL/min
Detector: ELSD
life science
0 3
6 9 12 15 18 21 24 Min.
Monoaromatics
Aromatic Acids
2
3
7
4
5 6
9 10
1. Uracil
2. Benzamide
3. Benzyl Alcohol
4. Phenol
5. Methylbenzoate
6. Acetophenone
7. N,N-Diethyl-m-toluamide
8. Toluene
9. Benzophenone
10. Biphenyl
8982
1
3
2
4 5
1. dl-4-Hydroxy-3-methoxy-mandelic Acid
2. Homovanillic Acid
3. Vanillic Acid
4. Salicylic Acid
5. Benzoic Acid
7373
1
8
0 1
2
3
4 Min.
0 1 2 3 4 5 6 7 8 9 10 11 Min.
Column: Alltech ® Platinum EPS C18, 3µm, 53 x 7mm Rocket
(Part No. 50573)
Mobile Phase: 0.05M KH2PO4, pH3.0:Methanol (50:50)
Flow Rate: 2.3mL/min
Detector: UV at 254nm
Column: Alltech ® Alltima C18, 5µm, 150 x 4.6mm (Part No. 88052)
Mobile Phase: 0.05M KH2PO4, pH3.2:Methanol (60:40)
Flow Rate: 1.0mL/min
Detector: UV at 254nm
1
2 3
4
Benzyl Acids
5
9538
1. 3-Amino-4-hydroxybenzoic Acid
2. 4-Hydroxybenzoic Acid
3. Benzoic Acid
4. 4-Phenylbutyric Acid
5. 5-Phenylbutyric Acid
1
2 3
4
5
6
7
8
10
9
11
Alcohols
12
1. Glycerol
2. Methanol
3. Ethanol
4. Isopropanol
5. tert-Butanol
6. Propanol
7. sec-Butanol
8. Isobutanol
9. tert-Pentanol
10. Butanol
11. Isopentanol
12. Pentanol
5440
0 2 4 6 8 10 12 14 Min.
Column: Alltech ® Prevail Organic Acid, 5µm, 150 x 4.6mm
(Part No. 88640)
Mobile Phase: 0.025M KH2PO4, pH2.5:Acetonitrile (60:40)
Flow Rate: 1.0mL/min
Detector: UV at 254nm
0 4 8 12 16 20 24 28 Min.
Column: Alltech ® OA-1000, 9µm, 300 x 6.5mm (Part No. 9046)
Mobile Phase: 0.01N Sulfuric Acid
Flow Rate: 0.8ml/min
Column Temp: 65°C
Detector: RI
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