Grace Catalog 2008

0
857
1714
2571
3428
4285
5142
6000
6856
7713
8570
9427
10284
11141
11998
12855
13712
14569
15426
16283
17140
analytical hplc
hplc columns | small molecule
Grace ® VisionHT Ultra High-Pressure Columns
See Separations with Speed and Clarity
• Ultra-fast separations with superior efficiency, sensitivity, and resolution
• Exceptional stability for long column lifetimes
• Comprehensive sub 2μm stationary phase offering
• 12,000psig pressure rating compatible with all ultra high-pressure LC systems
Grace ® VisionHT columns offer a new level of performance in HPLC. The powerful combination of
high-strength 1.5µm media with ultra-low volume hardware, delivers new clarity to your separations
and maintains exceptional column lifetime. Complex samples resolve 95% faster with 4x greater
sensitivity when compared to traditional 2.1 x 150mm, 5µm columns. And with a wide variety of
phases available, the possibilities are endless.
2500 mAU
2000
1500
1000
500
2500 mAU
0
0
2000
0
0
2
1500
1
3
1000
4
500
1 2
2
3
2 4 6 8 10 12 14 Min.
Increase Speed by 95% and Gain 4x Better Sensitivity
Column: VisionHT C18, 1.5µm,
2.0 x 50mm
Flow Rate: 0.8mL/min
4
2500 mAU
2000
1500
1000
4 6 8 10 12 14 Min.
Column: Traditional C18, 5µm, 2.1 x 150mm
Flow Rate: 0.2mL/min
500
0
1
2
3
4
Impurities
not seen on
traditional
5µm columns.
0 0.5 1 1.5 2 Min.
1. Thiourea
2. Toluene
3. Propylbenzene
4. Butylbenzene
System: Agilent 1200
Mobile Phase: (45:55) 20mM Potassium Phosphate,
pH 2.5:Acetonitrile
Detector: UV at 254nm
Temperature: Ambient
Efficiency and Retention Times Remain Constant after Exposure to 12,000psig and 2000 Injections
Plates/Meter (000)
300
250
200
150
100
50
Column Stability Comparison, 50 x 2mm
VisionHT Column 1
VisionHT Column 2
VisionHT Column 3
VisionHT Column 4
Column Volumes
VisionHT Column 5
VisionHT Column 6
VisionHT Column 7
Competitor Sub 2µm Column
Competitor
Sub 2µm Column
1
2+3
1
2+3
4
4 6
5
5
0 2 4 6 8 Min.
6
1. Uracil
2. Pyridine
3. Phenol
4. Methyl Alanine
5. 4-Butyl Benzoic Acid
6. Toluene
Initial
FINAL
(After 2000 injections)
0 2 4 6 8 Min.
Column: 2.0 x 50mm
Mobile Phase: 50mM KH2PO4, pH 7.0:CH3CN (60:40)
Flow Rate: 1.0mL/min
High-pressure competitor sub 2µm column lost efficiency at routine
12,000psig pressures. VisionHT columns remain stable.
Before and after chromatograms show a constant level of
performance after 2000 injections.
34
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