Grace Catalog 2008

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prep | flash | tlc GraceAlpha Media A New Silica Generation new • Increased loading capacity • High resolution • High efficiency DAC packing • Produced in facility complaint to IPEC-PQG GMP guidelines • Ultimately reproducible • Mechanically strong GraceAlpha Silica Specifications Pore Size: 120Å Pore Volume: 0.95mL/g Surface Area: 325m 2 /g Chemical Purity:
prep | flash | tlc Vydac ® Media 300Å RP Protein Purification Pioneer Pioneered by Grace and produced by a unique process based on purified oranosilicate starting materials, Vydac ® silicas are well defined, high purity, totally porous separation media that meet the most demanding needs of preparative and process scale users. Proven in over two decades of applications for protein, peptide, and nucleic acid separations, Vydac ® 300Å reversed-phase media have excellent selectivity and reproducibility. Bulk Vydac ® adsorbents incorporate bonded phase chemistries identical to those in Vydac ® brand analytical and prep HPLC columns, thereby assuring economical method development and reliable, dependable scale-up for preparative and process chromatographic applications. Vydac ® TP 300A Silica Specifications Pore Size: 300Å Pore Volume: 0.6mL/g Surface Area: 90m 2 /g Particle Shape: Spheroidal Vydac ® 300Å Media MS TP Butylsilane Octylsilane Octadecylsilane Butylsilane Octylsilane Octadecylsilane Octadecylsilane Diphenylsilane Bonded Chemistry C4 214MS C8 208MS C18 218MS C4 214TP C8 208TP C18 218TP C18 monomeric 238TP 219TP Silica 101TP 10–15µm Particles 214MSB1015 208MSB1015 218MSB1015 214TPB1015 208TPB1015 218TPB1015 238TPB1015 219TPB1015 101TPB1015 15–20µm Particles 214MSB1520 208MSB1520 218MSB1520 214TPB1520 208TPB1520 218TPB1520 238TPB1520 219TPB1520 101TPB1520 20–30µm Particles 214MSB2030 208MSB2030 218MSB2030 214TPB2030 208TPB2030 218TPB2030 238TPB2030 219TPB2030 101TPB2030 Available in 10g increments. tech tip Vydac ® MS vs. Vydac ® TP Both phases are based on the same high purity 300Å silica. However, MS undergoes an additional treatment prior to bonding that reduces the residual polar activity of the silica surface. For many applications this results in improved resolution and an increase in protein recovery. prep | media Two-Stage Purification of a Synthetic 135-mer Although generally recommended for oligonucleotides up to 75 bases, Vydac ® 214TP columns have been used to purify much longer synthetic oligonucleotides. Here a 135-mer is purified by two stages of chromatography on a 214TP column—the first with the 5'-dimethoxytrityl protecting group still attached, causing strong retention, and the second after removal of the trityl group. Column: Vydac ® 214TP1010 C4, 10µm,10 x 250mm Flow Rate: 5mL/min Mobile Phase: A: 0.1 M Triethylammonium Acetate, pH 7.0 B: Acetonitrile Gradient: Crude: 0 to 60% B from 5 to 40min Deprotected: 0 to 20% B from 5 to 25min Detector: UV at 260nm Data courtesy of Joseph Kosmoski and Dr. Michael Smerdon, Dept. of Biochemistry and Biophysics, Washington State University, Pullman, WA, USA related products Looking for Vydac ® analytical columns? See pages 84–95. www.discoverysciences.com 177
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Grace Catalog 2008
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analytical hplc Evaporative Light S
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analytical hplc Alltech ® 3300 ELS
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analytical hplc ELSD Performance Be
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analytical hplc Alltech ® ELSD 800
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analytical hplc LC/MS Nitrogen Gene
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analytical hplc Autosamplers Alltec
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analytical hplc Column Heaters Allt
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analytical hplc HPLC Pumps Alltech
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analytical hplc HPLC Pumps Alltech
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analytical hplc Data Systems EZChro
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analytical hplc Analytical HPLC Col
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analytical hplc Grace (Jones) Genes
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analytical hplc Columns for Small M
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analytical hplc Grace ® HPLC Hardw
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analytical hplc System Type 2 Low V
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analytical hplc Grace ® VisionHT
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analytical hplc Grace ® VisionHT
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analytical hplc Alltech ® Alltima
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analytical hplc Alltech ® Prevail
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analytical hplc Alltech ® Prevail
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analytical hplc Reverse Elution Ord
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analytical hplc GraceSmart Columns
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analytical hplc Alltech ® Brava C
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analytical hplc Alltech ® Apollo
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analytical hplc Alltech ® Adsorbos
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analytical hplc Grom Sil Columns 0
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analytical hplc Grom Amino Acid Co
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analytical hplc Jones Genesis ® Co
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analytical hplc Alltech ® OPA Amin
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analytical hplc Vydac ® Venture ®
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analytical hplc Vydac ® 201TP (con
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analytical hplc Astec Chirobiotic
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analytical hplc Jordi Columns Norma
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analytical hplc Macherey-Nagel Nucl
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analytical hplc Polymer Labs Column
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analytical hplc Shodex ® ODP2 HP C
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analytical hplc ZirChrom ® Columns
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analytical hplc Vydac ® MS Introdu
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analytical hplc Vydac ® MS Columns
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analytical hplc Vydac ® Everest ®
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analytical hplc Vydac ® 214TP C4 V
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analytical hplc Vydac ® ProZap C1
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analytical hplc Vydac ® Venture ®
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analytical hplc Alltech ® ProSpher
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analytical hplc Alltech ® Anion Co
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analytical hplc Alltech ® Anion Co
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analytical hplc Hamilton ® Anion,
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analytical hplc Alltech ® Surfacta
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analytical hplc Empty HPLC Hardware
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analytical hplc Stainless Steel Fri
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analytical hplc Prefilters Direct-C
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analytical hplc Two-Piece High-Pres
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analytical hplc High-Pressure Adapt
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analytical hplc High- and Low-Press
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analytical hplc Low-Pressure Adapte
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analytical hplc Fitting and Accesso
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analytical hplc Mobile Phase Inlet
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Page 147 and 148: Prep | Flash | TLC Preparative Chro
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Page 153 and 154: prep | flash | tlc GraceAlpha Sili
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Page 157 and 158: prep | flash | tlc Vydac ® MS 300
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Page 167 and 168: prep | flash | tlc Axial SFC Column
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Page 173 and 174: prep | flash | tlc Multipacker ® P
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Page 179 and 180: prep | flash | tlc Davisil ® Media
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Page 183 and 184: prep | flash | tlc Pumps, Injectors
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Page 187 and 188: prep | flash | tlc GraceResolv Sil
Page 189 and 190: prep | flash | tlc TLC Plates Davis
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gas chromatography Heliflex ® AT
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gas chromatography Heliflex ® USP
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gas chromatography Heliflex ® AT
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gas chromatography Alltech Column G
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gas chromatography Astec Chiraldex
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gas chromatography SGE ® BPX Capil
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gas chromatography Alltech ® Custo
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gas chromatography Alltech ® GC Pa
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gas chromatography Alltech ® GC Pa
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gas chromatography Alltech ® Popul
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gas chromatography Pretested GC Pac
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gas chromatography Custom Coated Pa
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gas chromatography GC Supports Chro
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gas chromatography GC Packings Sili
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gas chromatography Graphitized Carb
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gas chromatography GC Packings Haye
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gas chromatography Capillary Connec
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gas chromatography Capillary Access
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gas chromatography Packing Accessor
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gas chromatography Flowmeters Aalbo
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gas chromatography Gas Leak Detecti
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gas chromatography Physical Charact
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gas chromatography Oxygen Traps Pro
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gas chromatography Alltech ® Gas P
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gas chromatography Purifier Accesso
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gas chromatography Gas Sampling Bag
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gas chromatography Gas Sampling Acc
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gas chromatography GC Ferrules (con
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gas chromatography Parker Fittings
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gas chromatography Swagelok ® Fitt
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gas chromatography Deactivated SGE
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gas chromatography Septa Introducti
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gas chromatography Septa General Pu
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gas chromatography Flow Control Val
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General Chromatography SPE . . . .
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general chromatography SPE Introduc
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general chromatography SPE Introduc
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general chromatography Alltech ® S
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general chromatography Alltech ® E
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general chromatography Alltech ® E
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general chromatography Alltech ® M
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general chromatography Alltech ® M
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general chromatography Alltech ® 9
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general chromatography GracePure S
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general chromatography Manifold Acc
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general chromatography Filtration I
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general chromatography Syringe Filt
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general chromatography 2mL Centrifu
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general chromatography Recommended
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general chromatography Individual S
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general chromatography Acylation Ac
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general chromatography Gas Standard
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general chromatography Special Gas
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general chromatography Hydrocarbons
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general chromatography Alltech ® I
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general chromatography Hamilton ®
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general chromatography Vici ® Prec
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general chromatography SGE ® Syrin
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general chromatography Popper Glass
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general chromatography Vial Introdu
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general chromatography 15x45mm 4mL
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general chromatography Vials by Aut
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general chromatography 12x32mm R.A.
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general chromatography 12x32mm Scre
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general chromatography 12x32mm Crim
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general chromatography Limited Volu
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general chromatography 15x45mm Crim
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general chromatography 20mm Seals a
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general chromatography Silanized Gl
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general chromatography Screw Thread
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general chromatography Aluminum Sea
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general chromatography Polypropylen
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general chromatography Mini-Vials,
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general chromatography EPA Vials fo
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general chromatography 96-Well Prod
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general chromatography Racks and St
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general chromatography Crimpers and
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general chromatography Flex-Connect
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general chromatography Low-Pressure
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general chromatography Glass-Lined
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general chromatography Tubing Cutte
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general chromatography Tools Magnif
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hplc applications Analgesics Anti-I
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hplc applications Antidepressants T
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hplc applications Barbiturates 2 4
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hplc applications Anesthetics 1 + 2
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hplc applications Anti-Asthmatics M
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hplc applications Antibiotics Antib
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hplc applications Bacitracin Bacitr
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hplc applications Taxols Taxol ® a
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hplc applications 0 Ginseng 1 2 Gin
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hplc applications Chinese Pharmacop
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hplc applications Vitamins 1 2 3 5
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hplc applications 1 2 b-Carotenes 3
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hplc applications Carbohydrates 1 C
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hplc applications 3 1 2 4 Conquista
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hplc applications Organic Acids 1 8
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hplc applications Oils / Lipids 2 S
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hplc applications Oils / Lipids 1 2
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hplc applications Proteins & Peptid
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hplc applications Purification of T
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hplc applications 1) Cystine 2) Cys
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hplc applications Miscellaneous Pho
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hplc applications 1 Organochlorine
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hplc applications Water Pollutants
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hplc applications Polymers Polyuret
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hplc applications Biofuels Triacylg
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hplc applications Fuels Petrochemic
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ic applications 2 Anions 13033 2 Ba
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ic applications 3 Anions with pHBA
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ic applications Cations Rapid Analy
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ic applications Surfactants 1 2 Sho
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gc applications Ephedra Standards v
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gc applications Organic Volatile Im
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gc applications Fast GC Drug Analys
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gc applications
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gc applications Semivolatiles Colu
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gc applications Volatiles 5,6 Aroma
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gc applications EPA Method 624 —
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gc applications Alcohols The Glenli
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gc applications Essential Oils 1 2
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gc applications FAMEs 1 3 4 5 Sesam
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gc applications Fuel Texas Crude Oi
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gc applications Gases Scott Mix 216
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gc applications Amines Polyamines 2
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gc applications Gases 1 5 MAPP Gas
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gc applications Solvents Volatile O
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gc applications Test Mixes AT-
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spe applications Diuretics Procedur
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spe applications Additives Procedur
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spe applications Pesticides Procedu
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spe applications PAHs Procedure usi
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