Grace Catalog 2008

hplc applications
food & beverage
Carbohydrates
1 2
Sugar Alcohols
3 4
1. iso-Erythritol
2. Xylitol
3. Mannitol
4. Maltitol
9352
0 5 10 15 20 25 30 35 40 45 Min.
0 2 4 6 8 10 12 Min.
4
5 6 4
7
1 2
Column: Alltech ® Prevail Carbohydrate ES, 250 x 4.6mm
Column: Alltech ® Prevail Carbohydrate ES, 5µm, 250 x 4.6mm
(Part No. 35101)
(Part No. 35101)
Mobile Phase: Acetonitrile:Water (70:30)
Mobile Phase: A: Acetonitrile B: Water
Flow Rate: 1.0mL/min
Gradient: Time: 0 60
Detector: ELSD
%B: 17 35
Flow Rate: 1.0mL/min
Detector: ELSD
Imported Ale
Beverage Additives
3
9351
5
9446
1. Fructose
1. Ascorbic Acid
2. Glucose
2. Saccharin
3. Maltose
2
3. Benzoic Acid
4. Maltotriose
3
4. Aspartame
5. Maltopentaose
5. Vanillin
6. Maltohexaose
7. Maltoheptaose
1
1
Wine Analysis - 2000 Sipaska Rosé
2 3 4
1. Fructose
2. Sorbitol
3. Glucose
4. Maltose
9474
0 5 10 15 20 25 30 35 40 45 50 Min.
Column: Alltech ® Prevail Carbohydrate ES, 5µm, 250 x 4.6mm
(Part No. 35101)
Mobile Phase: A: Acetonitrile B: Water
Gradient: Time: 0 50
%B: 20 35
Flow Rate: 1.0mL/min
Detector: ELSD
0 1 2 3 Min.
Column: Alltech ® Prevail C18, 3µm, 50 x 2.1mm (Part No. 43818)
Mobile Phase: Acetonitrile:0.05M Ammonium Acetate, pH5.4, (20:80)
Flow Rate: 0.3mL/min
Detector: UV at 230
Wine Analysis by Ion Exclusion
5411
Partial Digest: Complex Polysaccharide
from Plant Cell Wall
0
3 5
2
1
4
6
7
10
8 9
10 20 30 40 50 Min.
1. Citric Acid
2. Tartaric Acid
3. Glucose
4. Malic Acid
5. Fructose
6. Acetic Acid
7. Glycerol
8. Lactic Acid
9. Methanol
10. Ethanol
Column: Alltech ® IOA-1000, 9mm, 300 x 7.8mm (Part No. 9646)
Mobile Phase: 0.005N Sulfuric Acid
Flow Rate: 0.3mL/min
Column Temp: 60°C
Detector: RI
Partial Digest: Complex Polysaccharide from Plant Cell Wall
0 15 30 45 60 75 90 Min.
Columns: VYDAC 218TP54 C18, 5 µm, 4.6 mm i.d. x 250mm
Flow: Column: 1.0 mL/min Vydac ® C18, 5µm, 250 x 4.6mm (Part No. 218TP54)
Gradient: Linear, 7 7 to to 10% Methanol MeOH over over 60 min., 60 min., then to 25%
MeOH then at 90 to min 25% Methanol at 90 min
Detection: Flow Rate: Evaporative 1.0mL/min light-scattering detection (ELSD)
Detector: ELSD
420
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