Grace Catalog 2008

mAU
hplc applications
pharmaceuticals
Antibiotics
1
2
Antibiotics via HILIC
0 5 10 15 20 25 30 35 40 45 Min.
3
1. Amikacin
2. Tobramycin
3. Neomycin
10626
1 2 3 45 6 7
8
Column: Alltech ® Alltima HP HILIC, 5µm, 150 x 1) 4.6mm Bacampiccillin Column: Grace ® VisionHT C18-P, 1.5µm, 50 x 2.0mm
(Part No. 86464)
2) Methicillin
(Part No. 5139606)
Mobile Phase: Acetonitrile:Methanol:Water (87:11:2) 3) Benzylpenicillin Mobile Phase: A: 0.1% Formic Acid B: Methanol
Flow Rate: 1.0mL/min
4) Phenoxymethylpenicillin Gradient: Time: 0 1.4
Detector: ELSD
5) Phen-oxyethylpenicillin
%B: 20 100
6) Phenoxypropylpenicillin Flow Rate: 0.4mL/min
7) Cloxacillin Detector: UV at 280nm
Penicillin Antibiotics
8) Propicillin
9) Proicillin
10) Dicloxacillin
Analysis of Vancomycin
20
15
10
5
0
Sulfa Drugs
1. Sulfadiazine
2. Sulfathiazole
3. Sulfamerazine
4. Sulfamethazine
5. Sulfamethoxazole
6. Sulfaisoxazole
7. Sulfaquinoxilone
8. Sulfamethoxine
0 1 2 3 4 5 Min.
2
1. Bacampicillin
1. N-Dimethylvancomycin
2. Methicillin Penicillin Antibiotics
2
2. Vancomycin1) N-Dimethylvancomycin
1 3
3. Benzylpenicillin
10
2) Vancomycin
4
4. Phenoxymethylpenicillin
7
5. Phen-oxyethylpenicillin
6. Phenoxypropylpenicillin
Column phase: GROM-SIL ODS-7 pH, 4 µm
Column phase: GROM-SIL 100
Column size: 150 x 4.0 mm
5 6 7. Cloxacillin
8 9 Column size: 250 x 4 mm
8. Propicillin Eluent 50 mM NaH2PO4 pH 2.3
9. Proicillin
Eluent : 0.1% H PO / ACN = 15
Flow rate: 0.75 ml/min
10. Dicloxacillin
1
Flow rate: 1.0 ml/min
Pressure: 17 MPa
Pressure: 13 MPa
Temperature: 37°C
Temperature: ambient
Detection (UV): 220 nm
0 3 6 9 12 15 18 21 24 27 30 33 Min.
0 2 4 6 8 10 12 14 16 18 20 22 Min. Detection (UV): 210 nm
Injection: 8 µl (~ 0.13 mg/ml of each)
Column: Grom Sil ODS-7 pH, 4μm, 150 x 4mm
Column: Grom Sil 100 ODS-0 AB, 5μm, 250 x 4mm
Injection: 10 µl (10 µg/ml)
Mobile Phase: 50mM Monosodium Phosphate, pH2.3
Mobile Phase: 0.1% Phosphoric Acid:Acetonitrile (15:85)
Flow Rate: 0.75mL/min
Flow Rate: 1.0mL/min
Detector: UV at 220nm
Detector: UV at 210nm
10724
0 2
Column:
Mobile Phase:
Flow Rate:
Detector:
Erythromycin
8630
1
1. Erythromycin
4 6 8 10 Min.
Alltech ® Macrosphere 300 C4, 7µm, 250 x 4.6mm
(Part No. 71039)
Acetonitrile: 0.2M Ammonium Acetate in Water,
pH4.5 (95:5)
1.0mL/min
ELSD
mAU
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
-500000 0
Column:
Mobile Phase:
Flow Rate:
Detector:
Amoxicillin (U.S.P.)
0 1 2 3 4 5 6 7 Min.
GraceSmart C18 Column
Competitor 1 C18
Competitor 2 C18
Competitor 3 C18
Competitor 4 C18
GraceSmart C18, 5µm, 250 x 4.6mm
(Part No. 5138810)
0.05N Monobasic Potassium Phosphate, pH5.0:
Acetonitrile (96:4)
1.5mL/min
UV at 230nm
10697
406
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